Veterinary parasitology最新文献

{"title":"Smart farming with AI: Enhancing anemia detection in small ruminants","authors":"Sadia Farhana","doi":"10.1016/j.vetpar.2025.110627","DOIUrl":"10.1016/j.vetpar.2025.110627","url":null,"abstract":"","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110627"},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in silico studies of GST-inhibitory and larvicidal activities of alkaloids against Rhipicephalus microplus","authors":"Amanda Ponce Morais Cerqueira ,&nbsp;Matheus da Cunha Santos ,&nbsp;Gabriel Lima Soares Rezende ,&nbsp;Manoelito Coelho dos Santos Junior ,&nbsp;Mariana Borges Botura","doi":"10.1016/j.vetpar.2025.110628","DOIUrl":"10.1016/j.vetpar.2025.110628","url":null,"abstract":"<div><div>The resistance of <em>Rhipicephalus microplus</em> to the main commercial acaricides has stimulated research for new antiparasitic agents, including natural products as an alternative to eco-friendly control. Among the main classes of secondary metabolites of plants stand out the alkaloids, which are characterized by a variety of biological activities such as enzymatic inhibition. The enzyme Glutathione-<em>S</em>-transferase (GST) is an important catalyst of xenobiotic detox reactions in arthropods and represents a promising target in the acaricide development. This work aimed to associate <em>in silico</em> and <em>in vitro</em> assays for the identification of alkaloids with inhibitory action against GST from <em>R. microplus</em> (RmGST). The model of RmGST was designed using the SWISS-MODEL online server and validated by molecular dynamics. The molecular affinity of alkaloids with RmGST was assessed using the DOCK 6.9 program and the best molecules were employed in <em>in vitro</em> assays. The <em>in vitro</em> assays performed were enzymatic inhibition and larval immersion tests. Molecular dynamics was run with GROMACS for alkaloid selected from <em>in silico</em> and <em>in vitro</em> analyses. The RmGST model shown to be sterically and energetically acceptable. In molecular dynamics simulations, the 3D structure remains stable with Root Mean Square Deviation = 4.35 Å and Root Mean Square Fluctuation = 2.21 Å. All the 20 alkaloids tested in molecular docking analyses show molecular affinity to the RmGST. Colchicine and papaverine show the best GridScocre: −38.09 and −36.09 kcal/mol, respectively. In <em>in vitro</em> enzymatic evaluation, colchicine exhibited more activity (81.73 %, 0.5 mg/mL) in comparison to papaverine (27.8 %, 0.5 mg/mL). However, these alkaloids did not interfere <em>in vitro</em> with the viability of <em>R. microplus</em> larvae. The colchicine contributed to the stability of the RmGST model in molecular dynamics simulations and presented hydrophobic and hydrogen interactions with residues from the orthosteric site. The association of <em>in silico</em> and <em>in vitro</em> techniques allowed the identification of two potential RmGST inhibitors (colchinine and papaverine) capable of interacting in the active site of the enzyme. RmGST inhibitors can be used in association with acaricides for a more effective control of <em>R. microplus</em> reducing parasite resistance.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110628"},"PeriodicalIF":2.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic in vitro effects of albendazole and minocycline on the viability of hydatid cyst protoscoleces","authors":"Nafiseh Hajiheidari ,&nbsp;Hossein Amini Khoei ,&nbsp;Kobra Mokhtarian ,&nbsp;Ebrahim Saedi Dezaki","doi":"10.1016/j.vetpar.2025.110624","DOIUrl":"10.1016/j.vetpar.2025.110624","url":null,"abstract":"<div><div>Hydatid disease poses a major zoonotic threat, affecting human health and livestock. Although albendazole is the standard treatment, it may cause adverse effects at high serum levels. This study investigated the protoscolicidal efficacy of minocycline alone and in combination with albendazole, compared to albendazole monotherapy, on hydatid cyst protoscoleces from sheep liver. Protoscoleces were aseptically aspirated from the sheep livers containing hydatid cysts. A total of 30,000 protoscoleces were suspended in 300 µl of normal saline and exposed to various concentrations of minocycline (10, 25, 50, 75, 125, 250, and 500 µg/ml) and albendazole (31.25, 62.5, 125, 250, 500, 1000, and 2000 µg/ml) for 10, 30, and 60 min, and subsequently from 1 h up to 43 h of incubation, in triplicate. The viability of the protoscoleces was assessed using 0.1 % eosin staining. The combination of Minocycline and Albendazole significantly reduced protoscoleces viability (P &lt; 0.05). All the treatment groups demonstrated progressive reductions in cell viability over time. Notably, combination therapy reduced viability to zero at 22 h, wherase Albendazole and Minocycline alone reached 0 % and 1.3 % viability, respectively, at 43 h. The combination of minocycline and albendazole produced significantly, partially, stronger scolicidal activity than either monotherapy, achieving complete protoscolex mortality within ∼22 h compared with 43 h for albendazole and incomplete killing with minocycline alone (P &lt; 0.05). These findings suggest that this drug combination may enhance treatment efficacy for hydatid disease and warrants further in vivo evaluation.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110624"},"PeriodicalIF":2.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timing is everything – Choosing the right time to screen the herd for neosporosis","authors":"Sharon Tirosh-Levy ,&nbsp;Elena Blinder ,&nbsp;Jacob Joost Doekes ,&nbsp;Monica L. Mazuz","doi":"10.1016/j.vetpar.2025.110622","DOIUrl":"10.1016/j.vetpar.2025.110622","url":null,"abstract":"<div><div>Neosporosis is a major cause of abortions in cattle, leading to economic losses. As no effective treatment or vaccine is currently available, the only strategy to reduce the impact of neosporosis in endemic herds is implementation of control programs, based on serological surveillance. This study aimed to determine the optimal timing for serological testing. Sera were collected from 132 heifers at three farms at three months intervals, between the ages 5 and 28 months. <em>Neospora</em> serological status and antibody titers were evaluated by IFAT, and by ELISA on the first sampling. The agreement between ELISA and IFAT results was 89.9 % (Kappa=0.798). Overall <em>Neospora</em> seroprevalence ranged between 36 % and 66 % on different dates. The antibody titers of almost all heifers fluctuated over time. Of 91 heifers that were sampled on 5 occasions, 67 % maintained their serological status throughout the study. Most cases of negative heifers that become positive in one or more tests (19 of 21 heifers) were transient, while only three remained persistently infected in all further tests. Seropositivity was significantly lower in lactating cows than in pregnant cows or heifers. The results of the first sampling date were representative of the overall status on all dates, with an agreement of 94.5 % (Kappa=0.889). The results demonstrate how fluctuations in antibody titers may affect the sensitivity of serological surveillance. Sampling young heifers, prior to their first insemination may be advisable, allowing future discrimination between what appear to be new infection, which is mostly transient, and congenital or chronic infections, which are usually life-long. This information may assist in the implementation of effective control measures in the herd.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110622"},"PeriodicalIF":2.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition, acaricidal activity, and acetylcholinesterase inhibitory activity of two chemotypes of Eugenia stictopetala essential oil against Rhipicephalus (Boophilus) microplus","authors":"Antônia Fernanda Lopes da Silva ,&nbsp;Júlia Karla de Albuquerque Melo Xavier ,&nbsp;Marcos Bispo Pinheiro Camara ,&nbsp;Cáritas de Jesus Silva Mendonça ,&nbsp;Livio Martins Costa-Júnior ,&nbsp;Wesley Douglas Ribeiro ,&nbsp;Caio Pavão Tavares ,&nbsp;Eloisa Helena de Aguiar Andrade ,&nbsp;José Guilherme Soares Maia ,&nbsp;Wallyson André dos Santos Bezerra ,&nbsp;Alexandra Martins Santos Soares ,&nbsp;Cláudia Quintino da Rocha","doi":"10.1016/j.vetpar.2025.110620","DOIUrl":"10.1016/j.vetpar.2025.110620","url":null,"abstract":"<div><div><em>Rhipicephalus (Boophilus) microplus</em>, an ectoparasite affecting livestock in tropical and subtropical regions, causes significant economic losses worldwide. In this context, effective control alternatives based on natural products are essential. This study evaluated the chemical composition, acaricidal activity, acute toxicity, and acetylcholinesterase (AChe) inhibition of essential oils (EO) from two chemotypes of <em>Eugenia stictopetala</em> (EOEs-1 and EOEs-2). Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed that both chemotypes were rich in monoterpene hydrocarbons (64.6 %). EOEs-1 was characterized mainly by myrcene (60.4 %) and citral (neral: 11.0 %; geranial: 15.1 %), whereas EOEs-2 contained myrcene (59.3 %), geraniol (20.0 %), and linalool (14.1 %) as its major constituents. Acaricidal activity was assessed by Larval Immersion Test (LIT) against <em>R. microplus</em> larvae, including the isolade compounds citral, myrcene, geraniol, and linalool. EOEs-1 exhibited the strongest acaricidal activity (LC₅₀ of 1.798 mg/mL), with citral exhibiting similar potency (LC₅₀=1.639 mg/mL), while EOEs-2 showed an LC₅₀ of 2.376 mg/mL. Additionally, both essential oils were capable of inhibiting the native acetylcholinesterase (AChE) of <em>R. microplus</em>, with EOEs-1 showing the strongest activity and emerging as a promising candidate for the control of this ectoparasite.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110620"},"PeriodicalIF":2.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological and molecular tools for environmental surveillance of canine echinococcosis: Steps toward a sustainable diagnostic algorithm","authors":"Héctor Gabriel Avila ,&nbsp;Lorena Evelina Lazzarini ,&nbsp;Luciano Ritossa ,&nbsp;Vilma Disalvo ,&nbsp;Verónica Roxana Flores ,&nbsp;Erio curto ,&nbsp;Fabián Zanini ,&nbsp;Gustavo Pedro Viozzi ,&nbsp;María Victoria Periago ,&nbsp;Nora Beatriz PIerangeli","doi":"10.1016/j.vetpar.2025.110623","DOIUrl":"10.1016/j.vetpar.2025.110623","url":null,"abstract":"<div><div>Canine echinococcosis (CaEc) surveillance has evolved from necropsy and arecoline purgation to the detection of coproantigens (cELISA) and genomic copro-DNA (cPCR and cLAMP). Each technique has advantages and disadvantages regarding biosafety, ethics, and costs. In Argentina, there is no consensus on CaEc surveillance tools or their suitability for low- and medium-complexity laboratories. The aim of this work was to compare the performance of techniques with different targets for CaEc surveillance, including cELISA, nested cPCR, and two cLAMPEGSL (2.0 and 3.0). Environmental canine fecal samples (n = 127) from endemic areas were analyzed using the four methods. Overall, Positive and Negative Percent Agreement (OPA, PPA, NPA) were evaluated. Sensitivity and specificity of each technique, and general prevalence were estimated using a Bayesian latent class model (BLCA). Both cLAMPEGSL3.0 and cPCR techniques showed higher OPA and NPA values than the cELISA, a validated method with very high NPV. Sensitivity estimates for each technique were: cELISA: 78.8 % (95 % CI: 56–94 %); cPCR 87.9 % (66–98 %); cLAMPEGSL2.0 65.6 % (29–96 %) and cLAMPEGSL3.0 86.3 % (50–99 %). While specificities estimates were: cELISA 55.7 % (46–66 %); cPCR 64.7 % (55–74 %); cLAMPEGSL2.0 57.9 % (47–68 %) and cLAMPEGSL3.0 62.4 % (52–73 %). The estimated general prevalence of CaEc was 13.1 % (9–18 %). This is the first study conducted in Argentina to compare the performance of four techniques with different targets for CaEc surveillance. Sensitivity and specificity of each technique, and general prevalence were estimated using a Bayesian Latent Class Analysis (BLCA) model. Using a BLCA model, both cPCR and cLAMPEGSL3.0 showed the best estimated sensitivity and specificity values. These results provide control programs with molecular tools suitable for use in medium- and low-complexity laboratories.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"340 ","pages":"Article 110623"},"PeriodicalIF":2.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial genetic stability of Echinococcus granulosus s.s. across life stages and hosts in an experimental infection model.","authors":"Figen Celik, Muhammet Uslug, Sami Simsek","doi":"10.1016/j.vetpar.2025.110576","DOIUrl":"10.1016/j.vetpar.2025.110576","url":null,"abstract":"<p><p>Cystic echinococcosis, caused by Echinococcus granulosus sensu stricto (G1/G3), is a major zoonosis with a complex transmission cycle. This study aimed to evaluate the mitochondrial genetic stability of E. granulosus s.s. across different life stages and host species using a controlled experimental infection model. To achieve this, mitochondrial genetic variation was analyzed separately in protoscoleces (naturally infected sheep), adult worms (experimentally infected dogs), and hydatid cysts (experimentally infected lambs), to assess within- and between-group genetic stability. Mitochondrial gene regions CO1 (875 bp) and NADH1 (1009 bp) were amplified and sequenced. Phylogenetic, haplotype, and neutrality analyses revealed that all isolates clustered within a single monophyletic group. While CO1 showed moderate haplotype (Hd = 0.730) and low nucleotide diversity (π = 0.00267), NADH1 displayed higher haplotypic and nucleotide diversity (Hd = 0.983; π = 0.00876). Significantly negative Fu's Fs values for both markers suggested a recent demographic expansion, potentially driven by clonal amplification under low evolutionary pressure. Despite the presence of several haplotypes, no host- or tissue-specific genetic differentiation was observed. These findings demonstrate the genetic continuity of E. granulosus s.s. throughout its life cycle and confirm the suitability of mitochondrial markers for molecular tracking and epidemiological studies in endemic regions.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"110576"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of Glutathione-S-transferase levels as marker of hepatic damage in chronic fasciolosis in cattle.","authors":"Al-Hassan Mohammed Mostafa, Gehan Mohammed Sayed, Ali Ali Hassan Al-Ezzi, Alaa Eldin Kamal","doi":"10.1016/j.vetpar.2025.110577","DOIUrl":"10.1016/j.vetpar.2025.110577","url":null,"abstract":"<p><p>There are various parasite pathogens that infect cattle, buffaloes, sheep, and goats, with fasciolosis being one of the most common. This article established a glutathione s-transferase (GST) evaluation for Fasciola spp infection and explored its application value as diagnostic tool for assess the hepatic damage, linking it with histopathological findings and the lesion score for the degree of infection with Fasciola spp. Thirty-two animals of cattle species were assigned. The investigation gathered fecal samples for sedimentation counts, blood samples for serum (GST quantification), and two tissue samples from a fasciolosis-infected liver (one in formalin for histopathological examination and the other for homogenate preparation for GST estimation). The animals were divided into four groups (8 each): Severely infected group (SG), Moderate infected group (MoG), Mild infected group (MiG), and non-infected group (C-ve). In sedimentation, SG showed a significantly higher fecal egg count but lower serum and homogenate GST values compared to other groups, while serum and homogenate GST values were lower in SG and MoG than in other groups. MiG group had higher values than C-ve, MoG and SG groups, respectively. Furthermore, pathological lesion scores were gradually increased from low to high in groups viz. (MiG, MoG, and SG, respectively). Hepatic fasciolosis is still a big economic problem in the veterinary field. GST could assess hepatic damage in the case of chronic fasciolosis.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"110577"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of electrochemical LAMP-MB signal evaluation using screen-printed graphene electrodes for quantitative detection of paramphistome egg DNA in faeces sample.","authors":"Sirapat Nak-On, Thanawan Tejangkura, Weena Siangproh, Thapana Chontananarth","doi":"10.1016/j.vetpar.2025.110570","DOIUrl":"10.1016/j.vetpar.2025.110570","url":null,"abstract":"<p><p>LAMP is a highly sensitive technique and is a useful and applicable tool for DNA detection. This study presents and compares alternative evaluations of the PAR-LAMP for paramphistome DNA detection using electrochemical signal measurements of methylene blue (MB) on screen-printed graphene electrodes (SPGEs) among the other LAMP applications. Two LAMP-MB signal evaluations are (i) the dropping LAMP-MB mixture and (ii) MB-DNA probe on SPGEs. These assays revealed a decrease in the current change (∆I) for positive result using square wave voltammetry (SWV). The dropping LAMP-MB mixture evaluation showed a higher fold current change difference (∆∆I/I₀) than the other evaluation and showed that the positive and negative results can be significantly discriminated. The analytical specificity assay revealed that the target paramphistome DNAs were detectable by the dropping LAMP-MB mixture assay, leading to an increase of the ∆∆I, which was significantly higher than the negative LAMP (P < 0.05). For analytical sensitivity, the gradient DNA concentrations of two paramphistomes were used to construct calibration curves and standard linear regression equations, and these revealed the lowest detected DNA compared with the other LAMP applications, including agarose gel electrophoresis and colorimetry. The electrochemical evaluation can detect a paramphistome egg, as well as the contaminated egg in the host's faeces. In addition, the estimated DNA for a paramphistome egg was calculated using a faeces-interfered factor. This is the first known application of electrochemical assay for parasite egg detection and the DNA quantification in faeces. Therefore, the application of the electrochemical LAMP-MB measurement using SPGEs, particularly the dropping LAMP-MB mixture assay, presented an effective diagnostic tool for DNA quantification in faeces as clinical specimens.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"110570"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro maintenance of the avian ectoparasite, Dermanyssus gallinae and its ability to subsequently feed on hen hosts.","authors":"Francesca Nunn, David A Ewing, Kathryn Bartley, Javier Palarea-Albaladejo, Giles Innocent, Eliezer Ramos, Wan Chen, Daniel R G Price, Alasdair J Nisbet","doi":"10.1016/j.vetpar.2025.110571","DOIUrl":"10.1016/j.vetpar.2025.110571","url":null,"abstract":"<p><p>Dermanyssus gallinae is a haematophagous mite species of major concern in the egg industry across the world and there has been a recent surge in studies to find new control methods for this parasite. To provide mites for these experiments, D. gallinae is often raised on hens with the attendant welfare and ethical issues that this entails. Alternatively, mites are collected from infested farm buildings which can lead to variability in mite provenance and quality as well as biosecurity issues. To attempt to overcome these issues in mite supply, we describe a method for maintenance of an in vitro colony of D. gallinae. Mites were maintained, in vitro, for up to 12 weeks and were fed several times per week with goose blood as a food source. The expansion of the colony was monitored weekly and the biomass of mites increased linearly during the initial 8 weeks of culture. To determine the ability of such in vitro-raised mites to feed and thrive if they were exposed to a hen host, mites that had been maintained in this way were used in an \"on-hen mite feeding assay\" to establish any differences in mite feeding rates, fecundity and mortality between in vitro-raised mites and mites freshly collected from a poultry farm. Feeding rate comparisons were significantly-different between experimental repetitions (p < 0.001), demonstrating the repeatability issues associated with different batches of farm-caught mites. Significantly higher feeding rates on hens were observed for one comparison of farm-caught, compared to in vitro-raised, deutonymphs (p = 0.012) and for adult females (p = 0.002); but no significant difference between the mite sources was demonstrated in feeding rates for protonymphs (p = 0.608) or adult females (p = 0.715) in another experiment. Following on-hen feeding, there were no statistically significant differences between experiments, or between in vitro-raised or farm-caught fed mites, for mite mortality in any life stage or for egg laying.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"339 ","pages":"110571"},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}