两种化学型刺荆挥发油的化学成分、杀螨活性及对微小棘头虫的乙酰胆碱酯酶抑制活性

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-10-02 DOI:10.1016/j.vetpar.2025.110620

Antônia Fernanda Lopes da Silva , Júlia Karla de Albuquerque Melo Xavier , Marcos Bispo Pinheiro Camara , Cáritas de Jesus Silva Mendonça , Livio Martins Costa-Júnior , Wesley Douglas Ribeiro , Caio Pavão Tavares , Eloisa Helena de Aguiar Andrade , José Guilherme Soares Maia , Wallyson André dos Santos Bezerra , Alexandra Martins Santos Soares , Cláudia Quintino da Rocha

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引用次数: 0

摘要

微头鼻虫（Boophilus microplus）是一种影响热带和亚热带地区牲畜的外寄生虫，在世界范围内造成重大经济损失。在这种情况下，基于天然产品的有效控制替代品至关重要。本研究评价了两种化学型龙葵精油（EOEs-1和EOEs-2）的化学成分、杀螨活性、急性毒性和乙酰胆碱酯酶（AChe）抑制作用。气相色谱-质谱（GC-MS）分析显示，两种化学型均富含单萜烯烃（64.6 %）。eoe -1的主要成分为月桂烯（60.4 %）和柠檬醛（neral: 11.0 %;gerial: 15.1 %），而eoe -2的主要成分为月桂烯（59.3 %）、香叶醇（20.0 %）和芳樟醇（14.1 %）。采用幼虫浸渍试验（LIT）测定了分离物柠檬醛、月桂烯、香叶醇和芳樟醇对小夜蛾幼虫的杀螨活性。EOEs-1具有最强的杀螨活性（LC₅0为1.798 mg/mL），柠檬醛具有相似的效力（LC50=1.639 mg/mL），而EOEs-2的LC₅0为2.376 mg/mL。此外，这两种精油都能抑制微褐霉的乙酰胆碱酯酶（AChE），其中EOEs-1表现出最强的活性，有望成为控制微褐霉体外寄生虫的候选药物。

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Chemical composition, acaricidal activity, and acetylcholinesterase inhibitory activity of two chemotypes of Eugenia stictopetala essential oil against Rhipicephalus (Boophilus) microplus

Rhipicephalus (Boophilus) microplus, an ectoparasite affecting livestock in tropical and subtropical regions, causes significant economic losses worldwide. In this context, effective control alternatives based on natural products are essential. This study evaluated the chemical composition, acaricidal activity, acute toxicity, and acetylcholinesterase (AChe) inhibition of essential oils (EO) from two chemotypes of Eugenia stictopetala (EOEs-1 and EOEs-2). Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed that both chemotypes were rich in monoterpene hydrocarbons (64.6 %). EOEs-1 was characterized mainly by myrcene (60.4 %) and citral (neral: 11.0 %; geranial: 15.1 %), whereas EOEs-2 contained myrcene (59.3 %), geraniol (20.0 %), and linalool (14.1 %) as its major constituents. Acaricidal activity was assessed by Larval Immersion Test (LIT) against R. microplus larvae, including the isolade compounds citral, myrcene, geraniol, and linalool. EOEs-1 exhibited the strongest acaricidal activity (LC₅₀ of 1.798 mg/mL), with citral exhibiting similar potency (LC₅₀=1.639 mg/mL), while EOEs-2 showed an LC₅₀ of 2.376 mg/mL. Additionally, both essential oils were capable of inhibiting the native acetylcholinesterase (AChE) of R. microplus, with EOEs-1 showing the strongest activity and emerging as a promising candidate for the control of this ectoparasite.

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.