Mitochondrial genetic stability of Echinococcus granulosus s.s. across life stages and hosts in an experimental infection model.

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-10-01 Epub Date: 2025-08-08 DOI:10.1016/j.vetpar.2025.110576

Figen Celik, Muhammet Uslug, Sami Simsek

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Abstract

Cystic echinococcosis, caused by Echinococcus granulosus sensu stricto (G1/G3), is a major zoonosis with a complex transmission cycle. This study aimed to evaluate the mitochondrial genetic stability of E. granulosus s.s. across different life stages and host species using a controlled experimental infection model. To achieve this, mitochondrial genetic variation was analyzed separately in protoscoleces (naturally infected sheep), adult worms (experimentally infected dogs), and hydatid cysts (experimentally infected lambs), to assess within- and between-group genetic stability. Mitochondrial gene regions CO1 (875 bp) and NADH1 (1009 bp) were amplified and sequenced. Phylogenetic, haplotype, and neutrality analyses revealed that all isolates clustered within a single monophyletic group. While CO1 showed moderate haplotype (Hd = 0.730) and low nucleotide diversity (π = 0.00267), NADH1 displayed higher haplotypic and nucleotide diversity (Hd = 0.983; π = 0.00876). Significantly negative Fu's Fs values for both markers suggested a recent demographic expansion, potentially driven by clonal amplification under low evolutionary pressure. Despite the presence of several haplotypes, no host- or tissue-specific genetic differentiation was observed. These findings demonstrate the genetic continuity of E. granulosus s.s. throughout its life cycle and confirm the suitability of mitochondrial markers for molecular tracking and epidemiological studies in endemic regions.

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颗粒棘球绦虫在不同生命阶段和宿主间线粒体遗传稳定性的实验感染模型

囊性棘球蚴病是由严格感细粒棘球蚴（G1/G3）引起的一种主要的人畜共患病，其传播周期复杂。本研究旨在通过控制实验感染模型，评估颗粒棘球蚴在不同生命阶段和不同宿主物种间的线粒体遗传稳定性。为此，分别分析了原头节（自然感染的绵羊）、成虫（实验感染的狗）和包虫囊（实验感染的羔羊）的线粒体遗传变异，以评估组内和组间遗传稳定性。线粒体基因区域CO1（875 bp）和NADH1（1009 bp）扩增并测序。系统发育、单倍型和中性分析显示，所有分离株都聚集在一个单系群内。CO1表现为中等单倍型（Hd = 0.730）和低核苷酸多样性（π = 0.00267）， NADH1表现为较高的单倍型和核苷酸多样性(Hd = 0.983；π = 0.00876)。两种标记的Fu's f值均为显著负，表明最近的人口扩张，可能是在低进化压力下克隆扩增所致。尽管存在几个单倍型，但没有观察到宿主或组织特异性的遗传分化。这些发现证明了颗粒棘球蚴在其整个生命周期中的遗传连续性，并证实了线粒体标记在流行区分子跟踪和流行病学研究中的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.