Innovative diagnostic strategies for Taenia ovis infection: Exploring molecular, gene expression, oxidative stress, and histopathological insights

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-08-05 DOI:10.1016/j.vetpar.2025.110567

Mai A. Salem , Marwa M. Khalifa , Noha M. Bakry , Mohamed S. Kamel , Mawada Mohamed Ali , Ehab A. Fouad , Reem M. Ramadan

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引用次数: 0

Abstract

Taenia ovis is an economically significant cestode affecting sheep. Its larval stage, Cysticercus ovis, causes ovine cysticercosis, leading to substantial economic losses due to carcass condemnation and reduced meat quality. This study utilized a comprehensive approach to investigate the prevalence, phylogenetic characterization, immune response, oxidative stress markers, and histopathological changes associated with T. ovis infection in naturally infected sheep. Among 220 slaughtered sheep examined post-mortem, an overall prevalence of 18.63 % was observed, with infection rates higher in males (20.83 %) than in females (16 %). Seasonal variation demonstrated the highest prevalence in spring (41.46 %), followed by winter (29.27 %), autumn (17.07 %), and summer (12.20 %). Molecular characterization of T. ovis isolates, which is based on the mitochondrial cytochrome c oxidase subunit I gene, revealed a high genetic similarity between Egyptian isolates and those from New Zealand, indicating genetic stability across geographically distinct regions. Immune response profiling showed significant upregulation (P < 0.0001) of cytokines such as interleukin 1β, interleukin 10, transforming growth factor β, interleukin 5, toll-like receptor 7, and gata-binding protein 3, indicating a robust inflammatory and immune-modulatory response. Further analysis of oxidative stress markers revealed marked increases in malondialdehyde and total oxidant status, reflecting heightened oxidative damage, while elevated total antioxidant capacity suggested a compensatory antioxidant response. Histopathological examination confirmed the infection through the observation of granulomatous inflammation, fibrosis, myositis, and calcification in the affected tissues. These findings underscore the pathogenic impact of T. ovis and provide valuable insights into its biology, emphasizing the need for improved diagnostic and control strategies to mitigate its economic and agricultural burdens.

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禽带绦虫感染的创新诊断策略：探索分子，基因表达，氧化应激和组织病理学见解

羊带绦虫是一种影响绵羊的重要经济寄生虫。它的幼虫阶段，即卵巢囊虫，会引起羊囊虫病，由于胴体腐烂和肉质下降，导致巨大的经济损失。本研究利用综合方法调查了自然感染的绵羊中与卵巢绦虫感染相关的患病率、系统发育特征、免疫反应、氧化应激标志物和组织病理学变化。在220只死后检查的屠宰羊中，观察到总患病率为18.63 %，其中雄性感染率（20.83 %）高于雌性感染率（16 %）。春季患病率最高（41.46 %），其次为冬季（29.27 %）、秋季（17.07 %）和夏季（12.20 %）。基于线粒体细胞色素c氧化酶亚基I基因的T. ovis分离物的分子特征显示，埃及分离物与来自新西兰的分离物具有高度的遗传相似性，表明遗传稳定性跨越地理上不同的区域。免疫反应分析显示，白细胞介素1β、白细胞介素10、转化生长因子β、白细胞介素5、toll样受体7和gata结合蛋白3等细胞因子显著上调（P <； 0.0001），表明存在强大的炎症和免疫调节反应。氧化应激标志物的进一步分析显示丙二醛和总氧化状态显著增加，反映了氧化损伤的加剧，而总抗氧化能力的提高表明了代偿性抗氧化反应。组织病理学检查通过观察受累组织肉芽肿性炎症、纤维化、肌炎、钙化证实感染。这些发现强调了T. ovis的致病作用，并为其生物学提供了有价值的见解，强调了改进诊断和控制策略以减轻其经济和农业负担的必要性。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.