银纳米粒子在寄主-寄生虫体内生物积累和迁移的超微结构表征

IF 2.2 2区 农林科学 Q2 PARASITOLOGY
Fuad Rzayev , Eldar Gasimov , Ali Nasirov , Sarvinaz Hajiyeva , Mehri Seyidbeyli , Aladdin Eyvazov , Gunay Rzayeva
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引用次数: 0

摘要

在发展纳米技术的时代,研究纳米粒子在生态系统各个组成部分中的生物积累和迁移,以及它们在活生物体中引起的不同程度的病理是很重要的。本研究利用光镜和电镜观察了纳米颗粒在宿主和寄生虫体内的生物积累和迁移,重点研究了对家鹅机体造成严重危害的异线虫。用浓度为100 μg/ml(总容积为10 ml)的银纳米粒子(Ag NPs)给药于染有异速弓形虫的禽类。用组织学方法和电子显微镜对寄主的肠道、肝脏和骨骼肌进行了检查。结果表明,AgNPs在游离状态下的大小为9.03 ~ 23.82 nm(13.88 ± 0.48 nm),在寄生虫体内可达14 nm,在鸟类中不超过13 nm。纳米颗粒在寄生虫体内生物积累,引起病理改变。观察到AgNPs通过寄生虫的表皮组织迁移到假体腔器官。在AgNPs的作用下,鸟类的肠、肝和骨骼肌的结构元件发生了各种病理变化。纳米颗粒进入位于鹅肠粘膜下层血管腔内的红细胞细胞质,随后迁移到肝脏和横切肌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrastructural characterization of bioaccumulation and migration of Ag nanoparticles in host-parasite organisms
In an era of developing nanotechnologies, studying the bioaccumulation and migration of nanoparticles in various components of the ecosystem, and the varying degrees of pathology they cause in living organisms - is important. In the present study, the bioaccumulation and migration of nanoparticles in both the host and parasite were examined by light and electron microscopy, focusing on the nematode Heterakis dispar, which causes serious damage to the organism of the domestic goose. Silver nanoparticles (Ag NPs) were administered to birds infected with H. dispar at a concentration of 100 μg/ml (total volume 10 ml). The parasites, as well as intestine, liver, and skeletal striated muscle of the host, were examined by histological methods and electron microscopy. It was found that the sizes of AgNPs at the free state were ranging from 9.03 to 23.82 nm (13.88 ± 0.48 nm), while in the parasite organism they were up to 14 nm, and in birds they did not exceed 13 nm. Nanoparticles bioaccumulated in the parasite, causing pathological changes. AgNPs were observed to migrate through the integumentary tissue of the parasite into the pseudocoelomic cavity organs. Various pathological changes occurred in the structural elements of the intestine, liver, and skeletal striated muscle of birds due to the action of AgNPs. Nanoparticles entered the cytoplasm of erythrocytes located in the lumen of the vessels in the submucosal layer of the goose intestine and subsequently migrated to the liver and striated muscle.
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来源期刊
Veterinary parasitology
Veterinary parasitology 农林科学-寄生虫学
CiteScore
5.30
自引率
7.70%
发文量
126
审稿时长
36 days
期刊介绍: The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.
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