Viral immunology最新文献_第4页

Role of the SARS-CoV-2 Virus in Brain Cells. SARS-CoV-2 病毒在脑细胞中的作用

IF 2.2 4区医学

Viral immunology Pub Date : 2024-03-01 Epub Date: 2024-02-05 DOI: 10.1089/vim.2023.0116

Ali Azargoonjahromi

引用次数: 0

Evaluation of Adjuvant Effect of Cytosine-Guanosine-Oligodeoxynucleotide in Meat-Type Chickens Coadministered In Ovo with Herpesvirus of Turkey Vaccine. 评估胞嘧啶-鸟苷-寡脱氧核苷酸在肉用型鸡体内与火鸡疱疹病毒疫苗联合接种时的佐剂效应

IF 2.2 4区医学

Viral immunology Pub Date : 2024-03-01 Epub Date: 2024-02-01 DOI: 10.1089/vim.2023.0125

Allison C Boone, Raveendra R Kulkarni, Aneg L Cortes, Carissa Gaghan, Javid Mohammed, Tarsicio Villalobos, Javier Esandi, Isabel M Gimeno

{"title":"Evaluation of Adjuvant Effect of Cytosine-Guanosine-Oligodeoxynucleotide in Meat-Type Chickens Coadministered In Ovo with Herpesvirus of Turkey Vaccine.","authors":"Allison C Boone, Raveendra R Kulkarni, Aneg L Cortes, Carissa Gaghan, Javid Mohammed, Tarsicio Villalobos, Javier Esandi, Isabel M Gimeno","doi":"10.1089/vim.2023.0125","DOIUrl":"10.1089/vim.2023.0125","url":null,"abstract":"Herpesvirus of turkey (HVT) increases activation of T cells in 1-day-old chickens when administered in ovo. This study evaluated whether adding cytosine-guanosine oligodeoxynucleotides (CpG ODNs) to the HVT vaccine could enhance the adjuvant effect of HVT. We used a CpG ODN dose of 10 μg per egg. The experimental groups were (1) diluent-only control (sham), (2) HVT, (3) HVT+CpG ODN, (4) HVT+non-CpG ODN, (5) CpG ODN, and (6) non-CpG ODN control. Cellular response evaluation included measuring the frequencies of macrophages (KUL01+MHC-II+), gamma delta T cells (γδTCR+MHC-II+), CD4+, and CD8+ T cell subsets, including double-positive (DP) cells. In addition, CD4+ and CD8+ T cell activation was evaluated by measuring the cellular expression of major histocompatibility complex class II (MHC-II), CD44 or CD28 costimulatory molecules. An adjuvant effect was considered when HVT+CpG ODN, but not HVT+non CpG ODN, or CpG ODN, or non-CpG ODN, induced significantly increased effects on any of the immune parameters examined when compared with HVT. The findings showed that (1) HVT vaccination induced significantly higher frequencies of γδ+MHC-II+ and CD4+CD28+ T cells when compared with sham chickens. Frequencies of DP and CD4+CD28+ T cells in HVT-administered birds were significantly higher than those observed in the non-CpG ODN group. (2) Groups receiving HVT+CpG ODN or CpG ODN alone were found to have significantly increased frequencies of activated CD4+ and CD8+ T cells, when compared with HVT. Our results show that CpG ODN administration in ovo with or without HVT significantly increased frequencies of activated CD4+ and CD8+ T cells.","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"89-100"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139672764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Series of Articles About T Cells and Vaccines. 关于 T 细胞和疫苗的系列文章。

IF 2.2 4区医学

Viral immunology Pub Date : 2024-03-01 DOI: 10.1089/vim.2024.0020

Rodney S Russell

引用次数: 0

Epstein-Barr Virus BRLF1 Induces PD-L1 Expression in Nasopharyngeal Carcinoma Cells. 爱泼斯坦-巴氏病毒 BRLF1 可诱导鼻咽癌细胞表达 PD-L1。

IF 2.2 4区医学

Viral immunology Pub Date : 2024-03-01 DOI: 10.1089/vim.2023.0118

Chung-Chun Wu, Mei-Shu Chen, Ting-Ying Lee, Tze-Sing Huang, Der-Yang Cho, Jen-Yang Chen

{"title":"Epstein-Barr Virus BRLF1 Induces PD-L1 Expression in Nasopharyngeal Carcinoma Cells.","authors":"Chung-Chun Wu, Mei-Shu Chen, Ting-Ying Lee, Tze-Sing Huang, Der-Yang Cho, Jen-Yang Chen","doi":"10.1089/vim.2023.0118","DOIUrl":"10.1089/vim.2023.0118","url":null,"abstract":"Nasopharyngeal carcinoma (NPC) is a specific human malignancy with unique geographic distribution and genetic backgrounds. Although early treatment with radio-chemotherapy has been proven effective for NPC therapy, its therapeutic efficacy substantially diminishes in the late stages of this malignancy. In the tumor microenvironment of NPC, PD-L1 has been demonstrated as a critical factor in impairing T cell activation. As an etiological role for NPC development, it is found that Epstein-Barr virus (EBV) latent proteins upregulated PD-L1 expression. However, whether EBV lytic protein affects PD-L1 expression remains unclear. In this study, through monitoring the mRNA expression pattern of lytic genes and PD-L1 in EBV-positive NPC cell line NA, EBV immediately-early gene BRLF1(Rta) was found to have the potential for PD-L1 activation. Furthermore, we identified that Rta expression enhanced PD-L1 expression in mRNA and protein levels through quantitative real-time polymerase chain reaction and western blotting analysis. The luciferase reporter assay revealed that Rta expression enhanced PD-L1 promoter activity. We also demonstrated that Rta-induced PD-L1 expressions could impair interleukin 2 secretion of T cells, and this mechanism may be through ERK activation. These results displayed the importance of EBV Rta in PD-L1 expression in NPC and may give an alternative target for NPC therapy.","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":"37 2","pages":"115-123"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140159134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Progress on the Weak Immune Response to the COVID-19 Vaccine in Patients with Type 2 Diabetes. 关于 2 型糖尿病患者对 COVID-19 疫苗的弱免疫反应的研究进展。

IF 2.2 4区医学

Viral immunology Pub Date : 2024-03-01 DOI: 10.1089/vim.2023.0097

Shiqi Yuan, Wenwen He, Bin Liu, Zhuoran Liu

引用次数: 0

Analysis of Serum Anti-HBs Levels and HBsAg/HBeAg Markers in Children and Adolescents: A Cross-Sectional Study. 儿童和青少年血清抗-HBs 水平和 HBsAg/HBeAg 标记物分析：一项横断面研究

IF 2.2 4区医学

Viral immunology Pub Date : 2024-03-01 Epub Date: 2024-03-06 DOI: 10.1089/vim.2023.0121

Zhili Niu, Pingan Zhang

{"title":"Analysis of Serum Anti-HBs Levels and HBsAg/HBeAg Markers in Children and Adolescents: A Cross-Sectional Study.","authors":"Zhili Niu, Pingan Zhang","doi":"10.1089/vim.2023.0121","DOIUrl":"10.1089/vim.2023.0121","url":null,"abstract":"Hepatitis B virus (HBV) is a global public health concern, and China continues to face a high burden of HBV cases. Vaccination plays a critical role in controlling and eradicating HBV. However, studies have shown that some individuals may experience waning immunity over time, highlighting the importance of enhanced immunization strategies. This study aimed to investigate the relationship between age, gender, and anti-HBs antibody levels, as well as the prevalence of serum hepatitis B surface antigen (HBsAg)/HBV e antigen (HBeAg) positivity. This retrospective study included 43,609 pediatric patients who visited the outpatient department between January 2013 and December 2022. Serum biomarkers (HBsAg, anti-HBs, HBeAg, anti-HBe, and anti-HBc) were measured using Roche Cobas 8000. There is a significant difference in anti-HBs titer between genders and across different age groups (p < 0.05). The serological markers HBsAg/HBeAg exhibited the highest positivity rate in the age group of 15-18 years. The findings demonstrate a gradual decrease in anti-HBs levels following HBV vaccination. The prevalence of serum markers HBsAg/HBeAg is higher among adolescents aged 15-18 years, which should be a matter of concern and attention.","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"107-114"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular Immune Responses Against γ-Inactivated Antigen in the Recovered Cases of Kyasanur Forest Disease. 京沙努尔森林病康复病例中针对γ-活化抗原的细胞免疫反应

IF 2.2 4区医学

Viral immunology Pub Date : 2024-03-01 Epub Date: 2024-02-05 DOI: 10.1089/vim.2023.0107

Himanshu Kaushal, Ramesh S Kartaskar, Tushar Chiplunkar, Pragya D Yadav, Pradeep Awate, Varsha A Potdar, Mahesh M Khalipe, Chinmay Saraf, Anita M Shete, Rima R Sahay, Shalini Das, Sarwade Apurva Chandrakant, Kalichamy Alagarasu

{"title":"Cellular Immune Responses Against γ-Inactivated Antigen in the Recovered Cases of Kyasanur Forest Disease.","authors":"Himanshu Kaushal, Ramesh S Kartaskar, Tushar Chiplunkar, Pragya D Yadav, Pradeep Awate, Varsha A Potdar, Mahesh M Khalipe, Chinmay Saraf, Anita M Shete, Rima R Sahay, Shalini Das, Sarwade Apurva Chandrakant, Kalichamy Alagarasu","doi":"10.1089/vim.2023.0107","DOIUrl":"10.1089/vim.2023.0107","url":null,"abstract":"Kyasanur Forest Disease Virus (KFDV) is a tick-borne flavivirus that causes life-threatening hemorrhagic fever in humans with case fatality rates of 3-5%. Relatively little is known about the mechanism of its pathogenesis or host immune responses to KFDV infection. Here, we investigated KFDV-specific cellular immune responses in the recovered cases of Kyasanur Forest Disease (KFD). Peripheral blood mononuclear cells of the recovered KFD cases and healthy controls were exposed to γ-inactivated KFDV antigen ex vivo. The proliferation index was determined using an enzyme-linked immunosorbent assay-based lymphoproliferative assay. The frequencies of CD4+ and CD8+ T cells expressing intracellular interferon (IFN)-γ in response to stimulation with γ-inactivated KFDV antigen were determined using flow cytometry. A significant increase in lymphoproliferation and a high frequency of CD4+ and CD8+ T cells secreting IFN-γ against γ-inactivated KFDV antigen were found in the recovered KFD group compared to the healthy control group. In conclusion, the study indicated the generation of cellular immune responses in individuals who recovered from KFD and can be used as indicators of cellular immunity in KFD vaccine studies.","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"101-106"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139693030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arexvy: A Comprehensive Review of the Respiratory Syncytial Virus Vaccine for Revolutionary Protection. Arexvy：全面回顾呼吸道合胞病毒疫苗带来的革命性保护。

IF 2.2 4区医学

Viral immunology Pub Date : 2024-01-01 Epub Date: 2024-02-05 DOI: 10.1089/vim.2023.0093

M Swathi

引用次数: 0

The Role of Long Noncoding RNA Negative Regulator of Interferon Response in the Regulation of Hantaan Virus Infection. 长非编码 RNA 干扰素反应负调控因子在调控汉坦病毒感染中的作用

IF 2.2 4区医学

Viral immunology Pub Date : 2024-01-01 Epub Date: 2024-02-06 DOI: 10.1089/vim.2023.0111

Yan Liang, Jiayi Zhan, Hongyan Shi, Wei Ye, Kaixuan Zhang, Jiayu Li, Wei Wang, Pingzhong Wang, Ying Zhang, Jianqi Lian, Xuyang Zheng

{"title":"The Role of Long Noncoding RNA Negative Regulator of Interferon Response in the Regulation of Hantaan Virus Infection.","authors":"Yan Liang, Jiayi Zhan, Hongyan Shi, Wei Ye, Kaixuan Zhang, Jiayu Li, Wei Wang, Pingzhong Wang, Ying Zhang, Jianqi Lian, Xuyang Zheng","doi":"10.1089/vim.2023.0111","DOIUrl":"10.1089/vim.2023.0111","url":null,"abstract":"Hantaan virus (HTNV) is prevalent in Eurasia. It causes hemorrhagic fever with renal syndrome (HFRS). Long noncoding RNAs (lncRNAs) play key roles in regulating innate immunity. Among these, lncRNA negative regulator of interferon response (NRIR) was reported as an inhibitor of several interferon (IFN)-stimulated genes. Our results showed that: NRIR expression was upregulated by HTNV infection in a type I IFN-dependent manner. The expression of NRIR in CD14+ monocytes from HFRS patients in acute phase was significantly higher than that in convalescent phase and healthy controls. HTNV infection in some HTNV-compatible cells was promoted by NRIR. NRIR negatively regulated innate immunity, especially IFITM3 expression. Localized in the nucleus, NRIR bound with HNRNPC, and knockdown of HNRNPC significantly weakened the effect of NRIR in promoting HTNV infection and restored IFITM3 expression. These results indicated that NRIR regulates the innate immune response against HTNV infection possibly through its interaction with HNRNPC and its influence on IFITM3.","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"44-56"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139698425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunogenicity Persistence of a Third-Dose Homologous BBIBP-CorV/CoronaVac Boosting Vaccination: A Prospective Open-Label Study. 第三剂同源 BBIBP-CorV/CoronaVac 增强疫苗的免疫原性持续性：一项前瞻性开放标签研究。

IF 2.2 4区医学

Viral immunology Pub Date : 2024-01-01 Epub Date: 2023-12-18 DOI: 10.1089/vim.2023.0075

Na Ren, Zhihong Wang, Sikang Gao

{"title":"Immunogenicity Persistence of a Third-Dose Homologous BBIBP-CorV/CoronaVac Boosting Vaccination: A Prospective Open-Label Study.","authors":"Na Ren, Zhihong Wang, Sikang Gao","doi":"10.1089/vim.2023.0075","DOIUrl":"10.1089/vim.2023.0075","url":null,"abstract":"The inactivated whole-virion severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine has been widely used in a two-dose schedule, but with insufficient data on the immunogenicity of homologous BBIBP-CorV/CoronaVac boosting vaccination and too little follow-up to assess the duration of the immunogenic response. We prospectively evaluated the immunogenicity of a third-dose BBIBP-CorV/CoronaVac boosting vaccination, with neutralizing titers against wild type and Omicron assessed at the baseline (immediately before the booster dose), and days 14, 28, 98, and 174 post the third-booster. Of 182 volunteers screened, 165 were assessed eligible for enrolment. No moderate/severe adverse events were observed during the term of the study. From the baseline to day 174 post the third booster, neutralizing titers against wild type and Omicron peaked by approximately sixfold increase (up to 811.83 and 33.40, respectively) at day 14 and slowly decreased over time. The geometric mean titers against Omicron were lower than against type with a 19.8-39. Sixfold reduction at all time points. The seropositivity against Omicron at the baseline, days 14, 28, 98, and 174 after the booster dose was 12.6%, 50.0%, 37.8%, 38.6%, and 22.8%, respectively. Data presented herein indicated that the BBIBP-CorV/CoronaVac booster significantly enhances the neutralizing potency against wild-type strain but elicited weaker neutralizing activity to Omicron. Our findings suggest that individuals receiving booster inactivated vaccine remain at risk for Omicron infection, which is crucial to inform ongoing and future vaccination strategies to combat coronavirus disease 2019.","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"16-23"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138810894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0