Viral immunology最新文献

{"title":"Vitamin D and Zinc in SARS-CoV-2 Infection: Immunomodulatory Mechanisms and Clinical Evidence.","authors":"Md Ekhlas Uddin, Md Asaduzzaman, Tasnim Ahmad, Sohel Ahmed, Sukalyan Kumar Kundu, Mohammad Mehedi Hasan Khan, Mohammad Mosaddequl Islam, Md Mosharraf Hossen, Md Ramjan Sheikh, Md Mominul Islam Sheikh","doi":"10.1177/08828245261426982","DOIUrl":"10.1177/08828245261426982","url":null,"abstract":"<p><p>The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in approximately 778 million reported cases and over 7 million deaths worldwide as of August 2025 (WHO COVID-19 Dashboard), predominantly due to variable acute and chronic lung infections accompanied by inflammatory responses within the pulmonary tract and vasculature. Despite ongoing research, no definitive cure has been identified. Preventive measures, including vaccines and monoclonal antibody-based interventions, have been developed to protect vulnerable populations, and hundreds of therapeutic candidates have been evaluated worldwide. Complementing these strategies, vitamin D and zinc (Zn) supplementation have emerged as promising, accessible adjunctive strategies due to their immunomodulatory and anti-inflammatory properties. This review synthesizes current experimental, clinical, and epidemiological evidence on the roles of vitamin D and Zn in modulating immune responses relevant to SARS-CoV-2 infection. Available data suggest that adequate vitamin D and Zn status may support immune function, reduce excessive inflammation, and potentially mitigate disease severity, particularly in deficient individuals. However, clinical trial outcomes remain heterogeneous. Overall, vitamin D and Zn supplementation may be considered supportive, adjunctive preventive measures. Further well-designed randomized controlled trials are required to define their optimal use in COVID-19 prevention and management.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"97-112"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147391123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and Evaluation of Anti-Sera Against Severe Acute Respiratory Syndrome Coronavirus 2 Variants Using a Syrian Hamster Model.","authors":"Yong Jun Choi, Bo Min An, Sang Won O, Jeong-Hyun Nam, Eun Ju Lee, Il-Hwan Kim, Jee Eun Rhee, Eun-Jin Kim","doi":"10.1177/08828245261424872","DOIUrl":"10.1177/08828245261424872","url":null,"abstract":"<p><p>The continuous emergence of novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants continues to influence the global coronavirus 2019 (COVID-19) pandemic. Even vaccinated individuals or those with prior infections may experience reinfection, depending on the immune evasion capacity of the circulating variants. Therefore, analyses of variant characteristics and immune escape are critical for informing public health policy. However, as COVID-19 has been downgraded in terms of infectious disease classification, the availability of patient sera has become increasingly limited, restricting the timely immunological analyses of emerging variants. To address this challenge, we established an antiserum production and neutralization assay system using a hamster model and demonstrated the utility of this animal serum-based approach. In this study, antisera against 10 circulating SARS-CoV-2 variants were generated in golden Syrian hamsters, and they showed high titer of humoral and neutralizing antibody using enzyme-linked immunosorbent assay and plaque reduction neutralization test (PRNT) assay. In addition, we compared serum from an unvaccinated patient (<i>n</i> = 1) infected with the BA.5 variant with serum from a BA.5-infected hamster (<i>n</i> = 1). Although the number of comparable samples was limited, the PRNT₅₀ titer patterns in serum from an unvaccinated individual infected with BA.5 and in hamster antisera were similar. These findings indicate that antisera generated in the hamster model can provide timely immunogenicity assessments of newly emerging SARS-CoV-2 variants, thereby contributing to the rapid characterization of variant immune escape and the generation of essential data for future public health preparedness.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"113-117"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of a Baculovirus-Expressed VP1-Based Immunoassay for BK Polyomavirus Serology and Preliminary Seroepidemiological Applications.","authors":"Negar Hemmati, Bahman Abedi Kiasari, Mohammedreza Hemmati","doi":"10.1177/08828245261424899","DOIUrl":"10.1177/08828245261424899","url":null,"abstract":"<p><p>BK polyomavirus (BKPyV) is highly prevalent and clinically relevant in immunocompromised populations, underscoring the need for reliable serological tools to support epidemiological monitoring and clinical follow-up. This study aimed to generate a recombinant VP1-based enzyme-linked immunosorbent assay (ELISA) using a baculovirus-Sf9 insect cell system and to explore its application in a human serum cohort. Recombinant VP1 was produced by baculovirus-mediated expression, purified by nickel-nitrilotriacetic acid affinity chromatography, and used to establish an indirect IgG ELISA. A plasmid-based transient expression approach in Sf9 cells was evaluated only as a feasibility experiment and did not yield sufficient VP1 for assay development. Analytical performance was assessed using a polymerase chain reaction (PCR)-defined serum panel (<i>n</i> = 30), and the assay was subsequently applied to 149 sera with unknown BKPyV status. Baculovirus-mediated expression generated high-purity VP1 with an average yield of approximately 1.55 mg/mL from a 10 mL culture. PCR-confirmed positive sera showed markedly higher optical density values than PCR-negative sera, with very good analytical discrimination in this small validation set (area under the curve = 0.996). In the cohort analysis, overall seroreactivity was 89.7%, antibody reactivity increased with age, and higher ELISA signals were observed in renal transplant and hemodialysis patients than in healthy individuals. Detectable reactivity in young children may reflect early exposure or passive maternal antibodies. These findings indicate that insect cell-derived BKPyV VP1 is a suitable antigen for serological assays and a practical platform for preliminary seroepidemiological applications, which require confirmation in larger independent validation cohorts.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"118-128"},"PeriodicalIF":1.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147285369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Immunomodulatory Roles of Extracellular Vesicles in the Pathogenesis of Virus-Related Cancers.","authors":"Sima Heidarzadeh-Asl, Fatah Kashanchi, Reza Jafari","doi":"10.1177/08828245251413728","DOIUrl":"10.1177/08828245251413728","url":null,"abstract":"<p><p>Since their discovery in the 1980s, extracellular vesicles (EVs) have garnered immense interest. These vesicles facilitate cell-to-cell communication and transfer physiologically active molecules such as proteins, lipids, and RNAs to target cells. Oncogenic viruses encode genes that enable viral replication and allow host cells to produce viral proteins and complexes. These viral components represent potential candidates for developing antiviral therapies or vaccines. Exosomes, a type of EV, can enhance immune responses by delivering immunostimulatory molecules and inhibiting viral replication. However, oncoviruses can also exploit the diverse immunoregulatory functions of exosomes to promote disease progression. This review focuses on the dual role of EVs produced during viral infections, examining how they can either enhance or suppress host immunity. We provide an overview of the function of exosomes in oncogenic virus infections, with a particular emphasis on their immunosuppressive and immunomodulatory potential. We also highlight challenges in harnessing these vesicles for advanced cancer theranostics and preventive strategies.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":"39 2","pages":"43-63"},"PeriodicalIF":1.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147310269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of IFN-γ and IFN-γ Receptor-1 Genes Polymorphisms with Hepatitis C Virus Infection Chronicity and Severity in Pakistani Patients.","authors":"Khair Rafiq, Sanaullah Khan, Muhammad Bar Khan, Muhammad Fawad Khan, Muhammad Yaqoob, Muhammad Adnan","doi":"10.1177/08828245251415180","DOIUrl":"10.1177/08828245251415180","url":null,"abstract":"<p><strong>Background: </strong>Hepatitis C is a chronic liver disease caused by infection with the hepatitis C virus (HCV), representing a major concern for global public health. The interferon gamma and interferon gamma receptor-1 (IFN-γR1) genes play a significant role in viral infections. This study aims to investigate the association between polymorphisms in the IFN-γ and IFN-γR1 genes and the chronicity of HCV infection.</p><p><strong>Methodology: </strong>This study included 310 participants, comprising 150 chronic Hepatitis C (CHC) patients and 160 healthy controls. Nucleic acids were isolated, and PCR was used for HCV detection. The intron-1 of IFN-γ and promoter-56 of IFN-γR1 genes were amplified through PCR and sequenced through Sanger's method.</p><p><strong>Results: </strong>The frequency of TT, AT, and AA genotypes of the IFN-γ at +874 in CHC patients was (20%), (57%), and (23%), while in healthy control, it was (46%), (38%), and (16%), respectively (<i>p</i> < 0.0001). In non-cirrhotic patients, they were (25.6%), (41.1%), and (33.3%); in cirrhotic patients, they were (26.3%), (39.5%), and (34.2%), while in HCC patients, they were (18.2%), (50%), and (31.8%). The AA genotype shows significant association with chronic liver complications (<i>p</i> = 0.017). The frequency of TT, TC, and CC genotypes of the IFN-γR1 gene at -56 was (21%), (43%), and (36%) in CHC patients, while in healthy individuals (38%), (21%), and (41%), (<i>p</i> < 0.0001). In non-cirrhotic patients, they were (30%), (49%), and (21%); in cirrhotic patients, they were (32%), (44%), and (24%), while in HCC patients, they were (22%), (46%), and (32%) (<i>p</i> = 0.027). The TC genotype is significantly associated with an increased risk of developing liver cirrhosis and HCC in CHC patients compared to healthy controls.</p><p><strong>Conclusion: </strong>The current study shows that the AA genotype of the IFN-γ and TC genotype of the IFN-γR1 genes are associated with increased susceptibility and HCV chronicity. While the TT genotype of the IFN-γ and the CC genotype of the IFN-γR1 may confer a protective effect.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":"39 2","pages":"72-80"},"PeriodicalIF":1.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147310200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Insights into Dengue Infection with Preliminary Emphasis on Serotype Distribution and Risk Factors in Karbi Anglong, Assam: A Cross-Sectional Study.","authors":"Tilenda Teronpi, Minerva Sarmah, Pallabi Sargiary, Arunjyoti Sarmah, Dipak Kumar Das, Longmindar Timung, Simanta Kalita","doi":"10.1177/08828245251415383","DOIUrl":"10.1177/08828245251415383","url":null,"abstract":"<p><p>Dengue is a viral illness primarily transmitted by <i>Aedes aegypti</i> and <i>Aedes albopictus</i>, which thrive in tropical and densely populated urban environments. Its rising global incidence has been linked to factors such as climate change, inadequate sanitation, and expanding urbanization. A dengue outbreak in Diphu, Assam, from 2022 to 2024 prompted this study, which aims to identify the circulating serotypes and assess associated risk factors. The study involved 7,805 symptomatic patients from Diphu Medical College and Integrated Disease Surveillance Program field visits. Patients with malaria or other viral infections were excluded. After obtaining informed consent, participants provided sociodemographic details and underwent NS1 antigen and IgM ELISA tests. RT-PCR was performed on all positive samples for dengue serotype identification. Among the suspected cases, 50.22% tested positive for dengue, of which 45.39% were acute and 4.83% were recent infections. Adults (56.12%), males (56.58%), tribal groups (56.37%), rural residents (50.88%), and individuals with lower education (50.0%) had higher infection rates. Preliminary investigation of 49 representative RT-PCR positive samples exhibited that DENV2 was the predominant serotype (57.1%), followed by DENV2&3 coinfections. Logistic regression showed increased risk associated with adult age, tribal ethnicity, rural residence, and coinfection, while higher literacy lowered the odds. The study highlights a high prevalence of dengue in Karbi Anglong and underscores the need for early diagnosis, community awareness, surveillance, and strengthened vector control measures to reduce the disease burden.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":"39 2","pages":"81-91"},"PeriodicalIF":1.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147310241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balancing Immunity and Disease: The Dual Role of Nuclear Factor-κB in Cellular Transformation and Viral Pathogenesis.","authors":"Salma Aktar, Saiful Amin","doi":"10.1177/08828245251415173","DOIUrl":"10.1177/08828245251415173","url":null,"abstract":"<p><p>Nuclear factor-kappa B (NF)-κB is recognized as the principal mediator of immunity due to its diverse roles in immune responses. The activation of NF-κB pathway induces the expression of multiple genes containing the consensus sequence that contributes not only to immune response against immediate early infection, but also to gene expression of viruses soon after infection, it engages at considerable steps during oncogenesis, and the adjustment of programmed cell death. Prolonged NF-κB activation is interlinked with acute inflammatory responses without directly driving cancer; however, the pathway also controls the expression of genes that contribute to hallmarks of cancer such as avoiding apoptosis, sustaining proliferation, and enabling replicative immortality.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":"39 2","pages":"64-71"},"PeriodicalIF":1.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147310213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics and Influencing Factors of Viral Respiratory Infection in Patients Undergoing Immunosuppressive Therapy.","authors":"Ming Chen, Na Li, Haifeng Yun, Guoxing Zhang, Rui Liu","doi":"10.1177/08828245251407696","DOIUrl":"10.1177/08828245251407696","url":null,"abstract":"<p><p>The clinical features of viral respiratory infections in individuals with rheumatic immune diseases, renal diseases, hematological diseases, and healthy individuals were analyzed. Additionally, the impact of immunosuppressive therapy on the clinical manifestations of viral respiratory infections was explored. Patients attending the outpatient clinic of the Department of Rheumatology and Immunology at Suzhou Hospital of Traditional Chinese Medicine from October 1, 2023, to May 1, 2024, as well as healthy individuals undergoing physical examinations, were selected for the study. Data were collected through questionnaires, including information on viral respiratory infections, vaccination status, clinical symptoms, and medication use. A total of 425 questionnaires were collected. There were significant differences among groups in recovery time, disease classification, vaccination rates, and specific clinical symptoms (<i>p</i> < 0.05). Immunosuppressive therapy significantly reduced the incidence of runny nose (<i>p</i> = 0.046) and chills (<i>p</i> < 0.001) but was associated with a higher proportion of ordinary cases, suggesting that it may be associated with increased disease severity (<i>p</i> = 0.012). Multivariate logistic regression analysis showed that the use of glucocorticoids significantly prolonged both recovery time (<i>p</i> = 0.007) and duration of cough (<i>p</i> = 0.044). The rheumatology group experienced shorter recovery time (<i>p</i> = 0.016) and a lower risk of fever (38°C-39°C), whereas the hematology group had an increased risk of high fever (>39°C; <i>p</i> = 0.037). Vaccination significantly increased the likelihood of mild cases (<i>p</i> = 0.041), serving as an important protective factor. Additionally, younger patients and females tended to exhibit relatively more severe clinical manifestations. Significant differences between the groups existed in terms of symptomatology, clinical symptoms, and time to recovery, and the use of immunosuppressive therapy, especially glucocorticoids, may have exacerbated the patient's condition. Vaccination provided a protective effect for patients undergoing immunosuppressive therapy, mitigating the condition to a certain extent.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":"39 1","pages":"26-36"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune and Virological Factors Influencing Human Respiratory Syncytial Virus Circulation and Increased Prevalence During and After the COVID-19 Pandemic.","authors":"Fela Mendlovic, Tanya Plett, Carlos Santiago-Olivares, Guillermina Avila-Ramírez, Ana Flisser, Evelyn Rivera-Toledo","doi":"10.1177/08828245251407618","DOIUrl":"10.1177/08828245251407618","url":null,"abstract":"<p><p>Human respiratory syncytial virus (hRSV) is a leading cause of respiratory infections in infants and older adults. The COVID-19 pandemic disrupted hRSV transmission due to non-pharmaceutical interventions (NPI), resulting in atypical circulation patterns, earlier seasonal peaks, and increased post-pandemic prevalence. Two key factors are proposed to underlie these changes: a reduced specific immune response due to decreased viral exposure and the emergence of novel hRSV variants. These factors contributed to a larger cohort of immunologically naïve children and lower levels of maternally derived antibodies, increasing susceptibility to severe hRSV disease, particularly in infants and children. Additionally, adults experienced waning immunity following prolonged periods of limited hRSV circulation. The post-pandemic resurgence was accompanied by the emergence of novel hRSV variants with altered transmissibility and virulence, such as GB5.0.6a in Europe and B.D.E.1 in China. These variants may reflect mutations driven by the reduced immunity, though further research is needed to assess their pathogenicity. Understanding the interplay between the reduced immunity due to NPI and virological factors is essential for addressing hRSV epidemiology. Enhanced molecular surveillance and immunological monitoring are crucial for guiding vaccination strategies and protecting vulnerable populations against future hRSV outbreaks.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"1-10"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145857153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of SARS-CoV-2-Specific IgM and IgG Antibodies in Serum Samples of Patients with COVID-19.","authors":"Neda Hampaiian, Asghar Tanomand","doi":"10.1177/08828245251407701","DOIUrl":"10.1177/08828245251407701","url":null,"abstract":"<p><strong>Background: </strong>Several lines of evidence have shown high intraindividual and intraindividual variability in serum levels of IgM and IgG antibodies in response to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The present study aimed to assess the levels of SARS-CoV-2-specific IgM and IgG antibodies in serum samples of laboratory-confirmed coronavirus disease 2019 (COVID-19) patients.</p><p><strong>Methods: </strong>The study included 286 patients diagnosed with COVID-19, with 118 males and 168 females. Serum samples were taken from all subjects at two different time points, including 2 and 4 weeks after the onset of symptoms. Serological levels of SARS-CoV-2-specific IgM and IgG was assessed using the enzyme-linked immunosorbent assay technique. The Student's <i>t</i>-test were used to compare the mean levels of SARS-CoV-2-specific IgM and IgG between groups.</p><p><strong>Results: </strong>We found that the mean serum levels of SARS-CoV-2-specific IgM and IgG in COVID-19 patients were increased in 4 weeks after symptom onset compared to 2 weeks earlier, but it was not statistically significant (<i>p</i> > 0.05). There was no significant sex-dependent difference in serological levels of SARS-CoV-2-specific IgM and IgG (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>Our findings suggest that the serum levels of SARS-CoV-2-specific IgM and IgG did not show significant differences depending on sex. Furthermore, serological levels of SARS-CoV-2-specific IgM and IgG did not significantly differ between 2- and 4-weeks following illness onset.</p>","PeriodicalId":23665,"journal":{"name":"Viral immunology","volume":" ","pages":"37-42"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145864720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}