Vox Sanguinis最新文献

{"title":"Lipaemic plasma: An objective non-invasive photometric method to classify plasma turbidity and its association with red cell haemolysis.","authors":"Lara A E de Laleijne-Liefting, Ido J Bontekoe, Johan W Lagerberg, Thomas R L Klei","doi":"10.1111/vox.13778","DOIUrl":"10.1111/vox.13778","url":null,"abstract":"<p><strong>Background and objectives: </strong>Plasma components are visually inspected, and non-transparent, turbid units are rejected for transfusion and fractionation. Additionally, in case a plasma component is deemed lipaemic, there is conflicting data on the accompanying red cell concentrate (RCC) in vitro quality. As visual inspection of plasma turbidity is a subjective method, we aimed to devise an objective measurement using a quick, non-invasive, table-top spectrophotometry-based method. Using this method, the correlation between spectrophotometric data and its predictive value on haemolysis of the accompanying RCC during storage was assessed.</p><p><strong>Materials and methods: </strong>A total of 365 plasma units were visually inspected for turbidity and analysed for light reflection parameters (L*, a* and b*) and triglyceride (TG) levels. Leukoreduced RCCs in saline-adenine-glucose-mannitol (SAGM), prepared from the accompanying lipaemic whole blood, were stored for up to 6 weeks and analysed for quality parameters.</p><p><strong>Results: </strong>The light reflection L* value was the most discriminating between clear and turbid/lipaemic plasma. Also, a correlation was found between TG levels and L* values (R² = 0.703). Plasma with TG ≥ 2.5 mmol/L showed an L* value >50 with >90% specificity and sensitivity. RCC from donations with a plasma L* value ≥68 showed significantly higher haemolysis levels (p < 0.05) during storage.</p><p><strong>Conclusion: </strong>The non-invasive photometric analysis of plasma turbidity correlated both with visual inspection and plasma TG levels. Measurement of L* values of plasma may be helpful in identifying donations with high TG levels and higher risk for increased haemolysis during RCC storage.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"277-283"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oropouche virus in Brazil: Assessing the risks and challenges for transfusion medicine.","authors":"Lucca Rocha Policastro, Debora Glenda Lima de La-Roque, Rodrigo Tocantins Calado, Dimas Tadeu Covas, Luiz Carlos Alcantara, Marta Giovanetti, Simone Kashima","doi":"10.1111/vox.13784","DOIUrl":"10.1111/vox.13784","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"256-259"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between blood test results and vasovagal reactions: An intra-individual comparative retrospective analysis of blood donor data in Japan.","authors":"Taeko Chijiiwa, Akie Hirata, Tasuku Okui, Junko Iwasaki, Naoki Nakashima, Midori Kumagawa","doi":"10.1111/vox.13787","DOIUrl":"10.1111/vox.13787","url":null,"abstract":"<p><strong>Background and objectives: </strong>To determine whether intra-individual differences in pre-donation blood test results were associated with vasovagal reactions (VVRs).</p><p><strong>Materials and methods: </strong>The study included donors who voluntarily donated 400 mL of whole blood at least twice during a 5-year blood collection period of the Japanese Red Cross, including both donations with and without a VVR. A conditional logistic regression analysis by age group and sex was conducted, using each donor as a stratum, together with an analysis of deviance to test the significance of including an interaction term between age group and blood data in the regression model.</p><p><strong>Results: </strong>A total of 1873 donors were included in the analysis. Haemoglobin, haematocrit, total protein and albumin values prior to donation were higher when a VVR was observed than when it was not for each age group and sex. The regression analysis showed significant positive associations between each of these blood parameters and VVR in all combinations of age groups and sex. A particularly strong positive association between haemoglobin and VVRs is seen in women aged ≥50 years (odds ratio, OR [95% confidence interval, CI]: 1.844 [1.457, 2.333]).</p><p><strong>Conclusion: </strong>Haemoglobin, haematocrit, total protein and albumin levels within individual donors were significantly higher at donation with a VVR. This was most notable in women aged ≥50 years. Comparing the pre-donation haemoglobin value with past values in the same donor at the donation site would help raise awareness of the risk of VVRs.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"260-267"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Forum on Genotyping of Blood Antigens in Donors: Summary.","authors":"Jennie Haw, Kelly Holloway, Gwen Clarke, Galit Eliyahu Nuni, Liora Muncher, James Daly, Jacqui Martin, Klaus Rieneck, Morten H Dziegiel, Cinzia Paccapelo, Luisa Ronzoni, Diarmaid O'Donghaile, Helen Ryan, Celina Montemayor Garcia, Cheuk-Kwong Lee, Wai-Chiu Tsoi, Hironori Kobayashi, Hiroyuki Igarashi, Tuulia Palukka, Katri Haimila, Nuria Nogues, Cecilia Gonzalez-Santesteban, Aaron J Gottschalk, Åsa Hellberg, Martin L Olsson, Thierry Peyrard, Ute Jentsch, Ai Leen Ang, Chew Yen Phang, Lorna Wall, Dhana Gounder, Nancy Dunbar","doi":"10.1111/vox.13790","DOIUrl":"10.1111/vox.13790","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"326-332"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and analysis of residual leucocytes from leucoreduced red blood cell units.","authors":"Rena Hirani, Melinda M Dean, David O Irving","doi":"10.1111/vox.13775","DOIUrl":"10.1111/vox.13775","url":null,"abstract":"<p><strong>Background and objectives: </strong>Leucoreduction is used to remove donor leucocytes during red blood cell (RBC) manufacture. However, not all are removed, and long-term survival of donor leucocytes, termed transfusion-associated microchimerism (TAM), has been shown to occur in some patients following RBC transfusion. The mechanism of TAM occurrence is unknown. One hypothesis is that viable donor haematopoietic cells remain within RBC units that could engraft. However, the analysis of cells remaining within leucoreduced RBC units has been minimal. This study aimed to isolate and analyse any residual leucocytes recovered from leucoreduced RBC units.</p><p><strong>Materials and methods: </strong>Leucoreduced RBC units were analysed on Day 1 (n = 4) and Day 42 (n = 4) post collection. Residual leucocytes were isolated using the EasySep™ RBC Depletion Reagent. Cell type analysis was conducted by flow cytometry using a leucocount reagent, a viability marker (7-amino-actinomycin D [7AAD]) and specific antibodies to CD45 and CD34. A representative 'pre-filter' sample was also obtained at the time of whole-blood donation to ensure expected cell counts across the donor samples.</p><p><strong>Results: </strong>Analysis of the pre-filter sample showed that CD45+/CD34+ cells accounted for 0.02%-0.07% of all leucocytes. Up to 253,850 residual leucocytes were isolated across both storage timepoints, and of these, up to 48 cells were CD45+/CD34+/7AAD-.</p><p><strong>Conclusion: </strong>Viable CD45+/CD34+ cells were isolated from leucoreduced RBC units, indicating the potential for donor progenitor cells to be present during transfusion. Further characterization of these residual cells is required to explain how TAM may occur in some patients following RBC transfusion.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"310-314"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal assessment of erythrogram parameters in response to granulocytapheresis frequency: A sex-based analysis.","authors":"Abdullah Alswied, Leonard N Chen, Kamille Aisha West-Mitchell","doi":"10.1111/vox.13788","DOIUrl":"10.1111/vox.13788","url":null,"abstract":"<p><strong>Background and objectives: </strong>Granulocyte transfusion supports patients with severe neutropenia. Maintaining a pool of eligible donors and optimizing donation frequency are essential for ensuring an adequate supply while safeguarding donor well-being. This study investigates the impact of donation frequency on erythrogram parameters, focusing on sex-specific differences.</p><p><strong>Study design and methods: </strong>We conducted a retrospective analysis of 343 successive granulocyte collections from 65 apheresis donors over 11 years (2012-2023). Donors were categorized by sex, and erythrogram parameters were analysed in relation to donation frequency and intervals.</p><p><strong>Results: </strong>Frequent donations within a short inter-donation interval (≥3 in 14 days) affected subsequent pre-donation haemoglobin levels. Each additional donation within 14 days led to a decrease of 0.81 g/dL in haemoglobin (p = 0.017). A significant interaction between sex and donations within 14 days (β = 0.76, p = 0.018) indicated that frequent donations had a more pronounced negative effect on haemoglobin levels in female donors. The proportion of donations meeting the pre-donation haemoglobin eligibility criteria declined with each successive donation within 14 days (100% at first, 85.8% at second, 25% at third). Female donors showed a significant haemoglobin reduction over three donations within 14 days (13.4-11.6 g/dL, p = 0.005) compared to males (14.4 -14 g/dL, p = 0.95).</p><p><strong>Conclusion: </strong>Short inter-donation intervals have a more pronounced negative effect on pre-donation haemoglobin levels in female donors, underscoring the need for individualized donation guidelines to ensure donor safety.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"268-276"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influenza and COVID-19 vaccination in Canadian blood donors: A comparison across pre- and post-pandemic periods.","authors":"Sheila F O'Brien, Lori Osmond, Mindy Goldman, Steven J Drews","doi":"10.1111/vox.70006","DOIUrl":"https://doi.org/10.1111/vox.70006","url":null,"abstract":"<p><strong>Background and objectives: </strong>Seasonal vaccinations reduce donor illness and appointment cancellations and ensure plasma products have antibodies to vaccine-directed strains. We aimed to describe donor influenza and COVID-19 vaccination history and compare this with the general population.</p><p><strong>Materials and methods: </strong>Two online donor surveys were carried out in 2021 and 2024. Donors were asked about demographics, influenza (2019/2020, 2020/2021 and 2023/2024 seasons) and COVID-19 (ever and 2023/2024 season) vaccination and reasons for vaccination choices. General population vaccination statistics were extracted from public reports. Percentages of donors receiving vaccination were calculated with 95% confidence intervals. Multiple logistic regression models were fitted with demographics as independent variables.</p><p><strong>Results: </strong>In survey 1, 4582 (30.4% response rate) donors completed a questionnaire; in survey 2, 6376 (21% response rate). More donors under age 65 received the influenza vaccine compared with the general population under age 65 (58% vs. 30% in 2019/2020, 63% vs. 28% in 2023/2024, p < 0.0001) and aged 65+ (81% vs. 70% in 2019/2020, 90% vs. 73% in 2023/2024, p < 0.0001). Fewer donors and the general population received the COVID-19 vaccine in 2023/2024 (under 65 45% vs. 39%; 65+ 76% vs. 67%, p < 0.0001). Most said they were vaccinated to prevent infection and protect others.</p><p><strong>Conclusion: </strong>Seasonal vaccination rates are higher in older donors, consistent with public health recommendations. Blood donors are more likely to be vaccinated against seasonal influenza than the general population, but post-pandemic uptake of the COVID-19 booster vaccine was low, more similar to the general population.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing syphilis cases in Japan: A risk posed to blood services.","authors":"H Suzuki, N H Tsuno, K Kitsukawa, T Miyagi, K Sakamoto, K Matsubayashi, M Satake, K Muroi","doi":"10.1111/vox.70005","DOIUrl":"https://doi.org/10.1111/vox.70005","url":null,"abstract":"<p><strong>Background and objectives: </strong>Syphilis cases surged in Japan, exceeding 10,000 in 2022, primarily through heterosexual transmission. The Japanese Red Cross Blood Services (JRCBS) screens donated blood for syphilis, disqualifying positive donors. This study explores syphilis infection among blood donors to understand its impact on blood collection and supply.</p><p><strong>Materials and methods: </strong>Data from blood donors (2015-2022) at JRCBS were analysed. Treponema pallidum (TP) antibody was tested by chemiluminescent enzyme immunoassay or chemiluminescent immunoassay. A total of 39,199,047 donations were scrutinized. Reactive samples were further tested by rapid plasma reagin (RPR).</p><p><strong>Results: </strong>The number of TP and RPR positives (referred as syphilis positives) in 2022 compared with 2015 increased by 1.7 times in blood donors and 4.9 times in the general population. High syphilis-positive rate in 2022 was mainly observed among male first-time/reactivated donors and females in their 20s and 30s. Although syphilis cases in general population declined during the Corona pandemic, no significant decrease occurred in syphilis-positive donors.</p><p><strong>Conclusion: </strong>Although the increase in syphilis positives among blood donors may be a reflection of the increase in the general population, the rate of increase was less pronounced among blood donors. Although the increase in the syphilis-positive rate may not affect blood product safety, it would have a significant impact on the number of potential blood donors in the future because the increase was more frequently observed in young donors and new donors, and currently, syphilis-positive individuals are permanently deferred from blood donation. The implementation of effective measures to prevent transmission in the general population is required.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A relational approach to understanding the factors influencing new plasma donor retention in Canada.","authors":"Morgan Seeley, Arooba Bari, Kelly Holloway","doi":"10.1111/vox.70002","DOIUrl":"https://doi.org/10.1111/vox.70002","url":null,"abstract":"<p><strong>Background and objectives: </strong>Understanding the experiences and perceptions of new plasma donors is important for developing strategies to retain them.</p><p><strong>Materials and methods: </strong>This qualitative study focuses on new donors' experiences with plasma donation, the factors that influence their interest in donating again and their thoughts about establishing a regular plasma donation routine. We conducted 48 one-on-one semi-structured interviews and used reflexive thematic analysis with a relational approach to donation to analyse these data.</p><p><strong>Results: </strong>For new plasma donors in this study, interest in returning to donate again was facilitated by relational care, where donors were cared for by attentive staff, and felt they could care for others by donating. Their interest in helping others through ongoing donation was influenced by their relationships with people who have benefited from blood products or experienced illnesses they associated with plasma-derived medicines, as well as their sense of social responsibility and community belonging. The most prevalent deterrent to donating again was the experience of feeling unwell during or after donation. The practice of relational care from staff members can mitigate the fallout of the negative experience. Retention of new donors requires flexibility to ensure that donation is easy, convenient and does not negatively impact their health and ability to care for others in their social network.</p><p><strong>Conclusion: </strong>Investigating retention decisions for new plasma donors through the lens of relational care provides insight that can help blood collection agencies develop more effective strategies for retention in non-remunerated settings.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding community perspectives for advancing inclusion of trans and gender-diverse people in blood donation in Australia.","authors":"Rachel Thorpe, Kyle Jensen, Barbara Masser, Peter Bentley, Tarin Dryden, Eleanor Forrest, Andy Kaladelfos, Travis Larcombe, Tanya Pastor, Aaron Saint-James, Shauna Wilson, Bridget Haire","doi":"10.1111/vox.70001","DOIUrl":"https://doi.org/10.1111/vox.70001","url":null,"abstract":"<p><strong>Background and objectives: </strong>Trans and gender-diverse people who wish to donate blood face significant barriers with policies and procedures differing across countries. It remains critical to explore the experiences and perspectives of trans and gender-diverse people in different locations to understand challenges in local systems. We undertook a qualitative study focussed on procedures and processes affecting trans and gender-diverse people in Australia, and their views about needed change.</p><p><strong>Materials and methods: </strong>Stakeholders collaborated with the researchers to refine the focus of and design the research. Semi-structured interviews were conducted with 14 trans and gender-diverse people who were current, past or potential donors. The interviews comprised open-ended questions about donation experiences, knowledge of current policies and procedures and preferences for change. Interviews were analysed using thematic analysis.</p><p><strong>Results: </strong>Participants reported varied and inconsistent donation experiences. When compared with current practice, participants preferred a two-step approach to donor registration that asks for sex reported at birth followed by gender identity. However, they also expressed concern that the two-step approach could deter new donors and stressed the importance of only collecting information relevant to eligibility assessment. Participants were supportive of a gender-neutral approach to assess eligibility to donate.</p><p><strong>Conclusion: </strong>Our study highlights significant barriers and procedural inconsistencies for trans and gender-diverse individuals when (considering) donating blood. We recommend more inclusive practices including clear communication about data use, rigorous staff training on gender diversity, registration processes that respect all gender identities and adopting a gender-neutral approach to donor screening.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}