Vox Sanguinis最新文献

{"title":"Development of a simplified platelet cryopreservation method: An in vitro investigation of reducing the DMSO concentration to allow administration without its pre-transfusion removal.","authors":"Lacey Johnson, Pearl Lei, Christopher Roan, Denese C Marks","doi":"10.1111/vox.13789","DOIUrl":"10.1111/vox.13789","url":null,"abstract":"<p><strong>Background and objectives: </strong>The most widely used method of platelet cryopreservation requires the addition of 5%-6% dimethylsulphoxide (DMSO), followed by its pre-freeze removal via centrifugation, to minimize toxicity. However, this adds complexity to the pre-freeze and post-thaw processing. Accordingly, the aim of this study was to simplify platelet cryopreservation by reducing the DMSO concentration and omitting the requirement for pre-transfusion removal.</p><p><strong>Materials and methods: </strong>Apheresis platelets were cryopreserved at -80°C according to standard blood-banking methods using 5.5% DMSO, with centrifugation, pre-freeze removal of DMSO and reconstitution in plasma following thawing (standard). In parallel, doses of DMSO (0%, 1.5%, 3%, 5.5%) were tested without centrifugation and reconstitution (no-wash). In vitro platelet quality was assessed by flow cytometry, aggregation, viscoelastic testing (thromboelastography [TEG]) and clot retraction.</p><p><strong>Results: </strong>Many in vitro platelet quality parameters showed DMSO dose dependency using the no-wash protocol (recovery, annexin-V, TEG maximum amplitude [MA]). Platelets frozen using the no-wash method with 3% DMSO showed a higher abundance of GPIbα (3% DMSO no-wash median fluorescence intensity [MFI]: 228 ± 16; standard MFI: 184 ± 16; p = 0.0016) and less degranulation (reduced P-selectin-positive platelets and concentration of supernatant P-selectin) than platelets frozen using the standard method. All functional properties measured were comparable to those of platelets frozen using the standard method.</p><p><strong>Conclusion: </strong>This study shows that improvements in cryopreserved platelet quality parameters can be obtained by removing the centrifugation processes (standard vs. 5.5% DMSO no-wash). A reduction in DMSO to 3% supports quality parameters, and if shown to be clinically acceptable, this cryopreservation method could improve platelet accessibility, as it is simpler and cheaper than the standard method.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":"120 3","pages":"284-292"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Forum on Genotyping of Blood Antigens in Donors: Responses.","authors":"Jennie Haw, Kelly Holloway, Gwen Clarke, Galit Eliyahu Nuni, Liora Muncher, James Daly, Jacqui Martin, Klaus Rieneck, Morten H Dziegiel, Cinzia Paccapelo, Luisa Ronzoni, Diarmaid O'Donghaile, Helen Ryan, Celina Montemayor Garcia, Cheuk-Kwong Lee, Wai-Chiu Tsoi, Hironori Kobayashi, Hiroyuki Igarashi, Tuulia Palukka, Katri Haimila, Nuria Nogues, Cecilia Gonzalez-Santesteban, Aaron J Gottschalk, Åsa Hellberg, Martin L Olsson, Thierry Peyrard, Ute Jentsch, Ai Leen Ang, Chew Yen Phang, Lorna Wall, Dhana Gounder, Nancy Dunbar","doi":"10.1111/vox.13791","DOIUrl":"10.1111/vox.13791","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"333-348"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing reagents and techniques for identifying RhCE variants in routine serological testing.","authors":"Tatiane Aparecida de Paula Vendrame, Carine Prisco Arnoni, Giovana Faria Devides, Nayara Moraes Silva, Afonso José Pereira Cortez, Flavia Roche Moreira Latini, Lilian Castilho","doi":"10.1111/vox.13792","DOIUrl":"10.1111/vox.13792","url":null,"abstract":"<p><strong>Background and objectives: </strong>Identifying RhCE variants is essential to prevent alloimmunization and manage complex cases. Unfortunately, these variants are often only detected after antibody formation, as they may go unnoticed in serological tests. This study aimed to assess monoclonal antisera using various methodologies to define the reactivity patterns of some variants by variable expression of RhCE antigens.</p><p><strong>Materials and methods: </strong>Samples were chosen based on atypical reactivity on routine RhCE typing of donors, screening of Afro-descendant donors using tube testing and patient samples with antibodies against their own antigens. All 53 samples were tested using tube, gel and microplate tests with five antisera. Antigen expression was assessed by flow cytometry, and RhCE variants were molecularly classified.</p><p><strong>Results: </strong>Tube test screening of African descent donors proved more effective in identifying a broad range of weak or partial antigens, particularly when using anti-e composed of MS-21, MS-16, MS63 clones and anti-c from the MS8011531019 clone. Automated instrument phenotyping successfully identified samples with RHCE*ceJAL allele, while most other variants were detected as positive (4+), similar to gel test, which intensified most reactions. When comparing methods and antisera for detecting variant e antigens, tube test identified a higher percentage of weak samples (63%-77%) compared with microplate (35%) and gel tests (14%).</p><p><strong>Conclusion: </strong>The results highlight the critical role of tube test in serological routines and the need to select clones capable of identifying RhCE variants. Detecting reduced RhCE antigen expression during routine serological testing can guide further molecular investigations and help prevent Rh alloimmunization.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"301-309"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Removing upper age restrictions for returning donors and increasing the new donor upper age: Novel adverse event findings using a comprehensive donor vigilance system in Australia.","authors":"Joanna Speedy, Gabrielle Josling, Veronica C Hoad","doi":"10.1111/vox.13776","DOIUrl":"10.1111/vox.13776","url":null,"abstract":"<p><strong>Background and objectives: </strong>In July 2019, Australia removed the upper age limit for returning donors (previously 80 years) and increased the upper age for new donors from 70 to 75 years. This study assessed the safety of our new policy for our newly eligible upper age donors (NEUADs).</p><p><strong>Materials and methods: </strong>For the period, 14 July 2019 to 30 June 2023, the relative risk (RR) of individual adverse events in NEUADs was compared with younger cohorts.</p><p><strong>Results: </strong>There were 4529 NEUADs who made 8000 donations. The overall rates of vasovagal reactions (VVRs) were significantly lower in the NEUAD cohort. However, compared with younger donors; returning NEUADs had a significantly higher rate of loss of consciousness (RR 2.62; 95% confidence interval [CI]: 1.41-4.86) and new NEUADs had a significantly higher rate of offsite VVRs (RR 1.60; 95% CI: 1.08-2.37). Both new and returning NEUADs had significantly higher rates of VVR requiring outside medical care (RR 2.48; 95% CI: 1.28-4.79 and RR 4.45; 95% CI: 2.00-9.91 respectively).</p><p><strong>Conclusion: </strong>Our findings support historical findings that overall VVR rates decline with age but suggest a higher risk of more serious VVRs.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"315-319"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restless legs syndrome among blood donors: A systematic review and meta-analysis.","authors":"Alain M Ngoma, Paulin B Mutombo, Mahmoud Mosli, Magot D Omokoko, Kenneth E Nollet, Hitoshi Ohto","doi":"10.1111/vox.13780","DOIUrl":"10.1111/vox.13780","url":null,"abstract":"<p><strong>Background and objectives: </strong>Restless legs syndrome (RLS), with adverse health outcomes, has been linked to blood donation, but evidence published thus far has not been rigorously analysed. This systematic review aggregates existing evidence on RLS among blood donors and identifies associated factors worthy of further investigation.</p><p><strong>Materials and methods: </strong>MEDLINE and EMBASE were searched for articles published through 16 December 2023. Eleven studies from eight countries were selected from 142 publications. The pooled prevalence of RLS was calculated using a random-effects model, with heterogeneity assessed by the Cochran Q and I² statistics. Meta-regression and sensitivity analyses explored sources of heterogeneity and the robustness of findings.</p><p><strong>Results: </strong>Eleven studies, involving 20,255 blood donors, were included. The pooled prevalence of RLS among blood donors was 10.30% (95% confidence interval [CI]: 5.54%-16.30%), which was significantly higher than in the general adult population (3.0%, 95% CI: 1.4%-3.8%). Meta-regression identified the year of study and geographical region as significant sources of heterogeneity. From the five studies that used logistic regression analyses, female sex and older age stand out as associated factors. No publication bias was detected, and sensitivity analysis confirmed the robustness of results.</p><p><strong>Conclusion: </strong>Our findings suggest a high burden of RLS among blood donors, underscoring the need for further research with standardized criteria, appropriate design and analytical methodologies to better understand the impact of RLS on individual donors and the global blood supply.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"246-255"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipaemic plasma: An objective non-invasive photometric method to classify plasma turbidity and its association with red cell haemolysis.","authors":"Lara A E de Laleijne-Liefting, Ido J Bontekoe, Johan W Lagerberg, Thomas R L Klei","doi":"10.1111/vox.13778","DOIUrl":"10.1111/vox.13778","url":null,"abstract":"<p><strong>Background and objectives: </strong>Plasma components are visually inspected, and non-transparent, turbid units are rejected for transfusion and fractionation. Additionally, in case a plasma component is deemed lipaemic, there is conflicting data on the accompanying red cell concentrate (RCC) in vitro quality. As visual inspection of plasma turbidity is a subjective method, we aimed to devise an objective measurement using a quick, non-invasive, table-top spectrophotometry-based method. Using this method, the correlation between spectrophotometric data and its predictive value on haemolysis of the accompanying RCC during storage was assessed.</p><p><strong>Materials and methods: </strong>A total of 365 plasma units were visually inspected for turbidity and analysed for light reflection parameters (L*, a* and b*) and triglyceride (TG) levels. Leukoreduced RCCs in saline-adenine-glucose-mannitol (SAGM), prepared from the accompanying lipaemic whole blood, were stored for up to 6 weeks and analysed for quality parameters.</p><p><strong>Results: </strong>The light reflection L* value was the most discriminating between clear and turbid/lipaemic plasma. Also, a correlation was found between TG levels and L* values (R² = 0.703). Plasma with TG ≥ 2.5 mmol/L showed an L* value >50 with >90% specificity and sensitivity. RCC from donations with a plasma L* value ≥68 showed significantly higher haemolysis levels (p < 0.05) during storage.</p><p><strong>Conclusion: </strong>The non-invasive photometric analysis of plasma turbidity correlated both with visual inspection and plasma TG levels. Measurement of L* values of plasma may be helpful in identifying donations with high TG levels and higher risk for increased haemolysis during RCC storage.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"277-283"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oropouche virus in Brazil: Assessing the risks and challenges for transfusion medicine.","authors":"Lucca Rocha Policastro, Debora Glenda Lima de La-Roque, Rodrigo Tocantins Calado, Dimas Tadeu Covas, Luiz Carlos Alcantara, Marta Giovanetti, Simone Kashima","doi":"10.1111/vox.13784","DOIUrl":"10.1111/vox.13784","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"256-259"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between blood test results and vasovagal reactions: An intra-individual comparative retrospective analysis of blood donor data in Japan.","authors":"Taeko Chijiiwa, Akie Hirata, Tasuku Okui, Junko Iwasaki, Naoki Nakashima, Midori Kumagawa","doi":"10.1111/vox.13787","DOIUrl":"10.1111/vox.13787","url":null,"abstract":"<p><strong>Background and objectives: </strong>To determine whether intra-individual differences in pre-donation blood test results were associated with vasovagal reactions (VVRs).</p><p><strong>Materials and methods: </strong>The study included donors who voluntarily donated 400 mL of whole blood at least twice during a 5-year blood collection period of the Japanese Red Cross, including both donations with and without a VVR. A conditional logistic regression analysis by age group and sex was conducted, using each donor as a stratum, together with an analysis of deviance to test the significance of including an interaction term between age group and blood data in the regression model.</p><p><strong>Results: </strong>A total of 1873 donors were included in the analysis. Haemoglobin, haematocrit, total protein and albumin values prior to donation were higher when a VVR was observed than when it was not for each age group and sex. The regression analysis showed significant positive associations between each of these blood parameters and VVR in all combinations of age groups and sex. A particularly strong positive association between haemoglobin and VVRs is seen in women aged ≥50 years (odds ratio, OR [95% confidence interval, CI]: 1.844 [1.457, 2.333]).</p><p><strong>Conclusion: </strong>Haemoglobin, haematocrit, total protein and albumin levels within individual donors were significantly higher at donation with a VVR. This was most notable in women aged ≥50 years. Comparing the pre-donation haemoglobin value with past values in the same donor at the donation site would help raise awareness of the risk of VVRs.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"260-267"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Forum on Genotyping of Blood Antigens in Donors: Summary.","authors":"Jennie Haw, Kelly Holloway, Gwen Clarke, Galit Eliyahu Nuni, Liora Muncher, James Daly, Jacqui Martin, Klaus Rieneck, Morten H Dziegiel, Cinzia Paccapelo, Luisa Ronzoni, Diarmaid O'Donghaile, Helen Ryan, Celina Montemayor Garcia, Cheuk-Kwong Lee, Wai-Chiu Tsoi, Hironori Kobayashi, Hiroyuki Igarashi, Tuulia Palukka, Katri Haimila, Nuria Nogues, Cecilia Gonzalez-Santesteban, Aaron J Gottschalk, Åsa Hellberg, Martin L Olsson, Thierry Peyrard, Ute Jentsch, Ai Leen Ang, Chew Yen Phang, Lorna Wall, Dhana Gounder, Nancy Dunbar","doi":"10.1111/vox.13790","DOIUrl":"10.1111/vox.13790","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"326-332"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and analysis of residual leucocytes from leucoreduced red blood cell units.","authors":"Rena Hirani, Melinda M Dean, David O Irving","doi":"10.1111/vox.13775","DOIUrl":"10.1111/vox.13775","url":null,"abstract":"<p><strong>Background and objectives: </strong>Leucoreduction is used to remove donor leucocytes during red blood cell (RBC) manufacture. However, not all are removed, and long-term survival of donor leucocytes, termed transfusion-associated microchimerism (TAM), has been shown to occur in some patients following RBC transfusion. The mechanism of TAM occurrence is unknown. One hypothesis is that viable donor haematopoietic cells remain within RBC units that could engraft. However, the analysis of cells remaining within leucoreduced RBC units has been minimal. This study aimed to isolate and analyse any residual leucocytes recovered from leucoreduced RBC units.</p><p><strong>Materials and methods: </strong>Leucoreduced RBC units were analysed on Day 1 (n = 4) and Day 42 (n = 4) post collection. Residual leucocytes were isolated using the EasySep™ RBC Depletion Reagent. Cell type analysis was conducted by flow cytometry using a leucocount reagent, a viability marker (7-amino-actinomycin D [7AAD]) and specific antibodies to CD45 and CD34. A representative 'pre-filter' sample was also obtained at the time of whole-blood donation to ensure expected cell counts across the donor samples.</p><p><strong>Results: </strong>Analysis of the pre-filter sample showed that CD45+/CD34+ cells accounted for 0.02%-0.07% of all leucocytes. Up to 253,850 residual leucocytes were isolated across both storage timepoints, and of these, up to 48 cells were CD45+/CD34+/7AAD-.</p><p><strong>Conclusion: </strong>Viable CD45+/CD34+ cells were isolated from leucoreduced RBC units, indicating the potential for donor progenitor cells to be present during transfusion. Further characterization of these residual cells is required to explain how TAM may occur in some patients following RBC transfusion.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"310-314"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}