Vox Sanguinis最新文献

{"title":"Isolation and analysis of residual leucocytes from leucoreduced red blood cell units.","authors":"Rena Hirani, Melinda M Dean, David O Irving","doi":"10.1111/vox.13775","DOIUrl":"10.1111/vox.13775","url":null,"abstract":"<p><strong>Background and objectives: </strong>Leucoreduction is used to remove donor leucocytes during red blood cell (RBC) manufacture. However, not all are removed, and long-term survival of donor leucocytes, termed transfusion-associated microchimerism (TAM), has been shown to occur in some patients following RBC transfusion. The mechanism of TAM occurrence is unknown. One hypothesis is that viable donor haematopoietic cells remain within RBC units that could engraft. However, the analysis of cells remaining within leucoreduced RBC units has been minimal. This study aimed to isolate and analyse any residual leucocytes recovered from leucoreduced RBC units.</p><p><strong>Materials and methods: </strong>Leucoreduced RBC units were analysed on Day 1 (n = 4) and Day 42 (n = 4) post collection. Residual leucocytes were isolated using the EasySep™ RBC Depletion Reagent. Cell type analysis was conducted by flow cytometry using a leucocount reagent, a viability marker (7-amino-actinomycin D [7AAD]) and specific antibodies to CD45 and CD34. A representative 'pre-filter' sample was also obtained at the time of whole-blood donation to ensure expected cell counts across the donor samples.</p><p><strong>Results: </strong>Analysis of the pre-filter sample showed that CD45+/CD34+ cells accounted for 0.02%-0.07% of all leucocytes. Up to 253,850 residual leucocytes were isolated across both storage timepoints, and of these, up to 48 cells were CD45+/CD34+/7AAD-.</p><p><strong>Conclusion: </strong>Viable CD45+/CD34+ cells were isolated from leucoreduced RBC units, indicating the potential for donor progenitor cells to be present during transfusion. Further characterization of these residual cells is required to explain how TAM may occur in some patients following RBC transfusion.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"310-314"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal assessment of erythrogram parameters in response to granulocytapheresis frequency: A sex-based analysis.","authors":"Abdullah Alswied, Leonard N Chen, Kamille Aisha West-Mitchell","doi":"10.1111/vox.13788","DOIUrl":"10.1111/vox.13788","url":null,"abstract":"<p><strong>Background and objectives: </strong>Granulocyte transfusion supports patients with severe neutropenia. Maintaining a pool of eligible donors and optimizing donation frequency are essential for ensuring an adequate supply while safeguarding donor well-being. This study investigates the impact of donation frequency on erythrogram parameters, focusing on sex-specific differences.</p><p><strong>Study design and methods: </strong>We conducted a retrospective analysis of 343 successive granulocyte collections from 65 apheresis donors over 11 years (2012-2023). Donors were categorized by sex, and erythrogram parameters were analysed in relation to donation frequency and intervals.</p><p><strong>Results: </strong>Frequent donations within a short inter-donation interval (≥3 in 14 days) affected subsequent pre-donation haemoglobin levels. Each additional donation within 14 days led to a decrease of 0.81 g/dL in haemoglobin (p = 0.017). A significant interaction between sex and donations within 14 days (β = 0.76, p = 0.018) indicated that frequent donations had a more pronounced negative effect on haemoglobin levels in female donors. The proportion of donations meeting the pre-donation haemoglobin eligibility criteria declined with each successive donation within 14 days (100% at first, 85.8% at second, 25% at third). Female donors showed a significant haemoglobin reduction over three donations within 14 days (13.4-11.6 g/dL, p = 0.005) compared to males (14.4 -14 g/dL, p = 0.95).</p><p><strong>Conclusion: </strong>Short inter-donation intervals have a more pronounced negative effect on pre-donation haemoglobin levels in female donors, underscoring the need for individualized donation guidelines to ensure donor safety.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"268-276"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Split red blood cell units contain defined extracellular K⁺ levels, which are improved by a washing procedure.","authors":"Fabiola Hoppe, Jacqueline Maier, Holger Kirsten, Martin Federbusch, Reinhard Henschler","doi":"10.1111/vox.70004","DOIUrl":"https://doi.org/10.1111/vox.70004","url":null,"abstract":"<p><strong>Background and objectives: </strong>We should control free K⁺ during massive transfusion (>80 mL/kg) of red blood cells (RBCs) in small children. To manage this, several national and international guidelines recommend using RBCs stored only up to 7 days. We tested a washing step for RBCs in saline-adenine-glucose-mannitol (SAGM) with or without irradiation to reduce supernatant K⁺ levels, improve quality and potentially extend the shelf life of stored RBCs.</p><p><strong>Materials and methods: </strong>RBCs of 240-330 mL were prepared from whole blood donations, then split into halves and stored in SAGM solution at 4 ± 2°C for 21 days. RBCs were split and washed on Days 1 and 8, and some were gamma-irradiated on Day 8. Glucose, lactate, lactate dehydrogenase (LDH), adenosine triphosphate (ATP), K⁺ and haemolysis were determined over 21 days.</p><p><strong>Results: </strong>After washing on Day 1, only glucose and lactate improved, whereas after washing on Day 8, LDH and K⁺ also improved. Irradiation resulted in accelerated K⁺ accumulation and increased haemolysis. Mean extracellular K⁺ concentrations were 21.2 ± 1.03 mM after irradiation on Day 8 versus 1.12 ± 0.05 mM after irradiation plus wash on Day 8, and 38.80 ± 2.13 mM on Day 10 after irradiation on Day 8 and 16.6 ± 0.05 mM on Day 10 after irradiation plus wash on Day 8.</p><p><strong>Conclusion: </strong>K⁺ concentrations remained <25 mM within 8 days of storage. We recommend irradiation by Day 8 at the latest for neonatal transfusion. The shelf life may be extended by another 48 h if the RBCs are also washed.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of pregnant women for foetal neonatal alloimmune thrombocytopenia: A cost-utility analysis.","authors":"Thijs W de Vos, Ilonka Tersteeg, Enrico Lopriore, Dick Oepkes, Leendert Porcelijn, C Ellen van der Schoot, E Joanne T Verweij, Dian Winkelhorst, Masja de Haas, M Elske van den Akker-van Marle","doi":"10.1111/vox.13779","DOIUrl":"10.1111/vox.13779","url":null,"abstract":"<p><strong>Background and objectives: </strong>Foetal and neonatal alloimmune thrombocytopenia (FNAIT) results from maternal platelet-directed antibodies and can result in severe intracranial haemorrhage (ICH) in foetuses and newborns. Screening for human platelet antigen-1a (HPA-1a)-directed antibodies during pregnancy could allow timely intervention with antenatal treatment and prevent ICH. We assessed the cost effectiveness of HPA-1a typing and anti-HPA-1a-screening as part of the prenatal screening programme.</p><p><strong>Materials and methods: </strong>Different HPA-1a screening scenarios were tested in a decision analysis model and assessed for diagnostic, treatment, intervention and lifetime costs and prevention effects compared to the current situation without screening in the Netherlands. Model parameters were based on available data, literature and expert opinions. One-way sensitivity analysis and probabilistic sensitivity analysis were performed.</p><p><strong>Results: </strong>Adding screening for anti-HPA-1a antibodies to the current antenatal screening programme of the Netherlands will lead to an additional cost of €4.7 million per year and a gain of 226 quality-adjusted life years (QALYs) per year, indicating an incremental cost-effectiveness ratio (ICER) of €20,782 per QALY gained. One-way sensitivity analysis showed that the uncertainty around the incidence of ICH, lifetime costs of disabled children and the probability of having antibody quantitation >3.0 IU/mL at 20 weeks had the highest effect on the ICER.</p><p><strong>Conclusion: </strong>Antenatal anti-HPA-1a screening might be cost effective. To obtain more knowledge and thereby to improve risk stratification, a pilot screening programme is warranted.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"178-187"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of wrong blood in tube events at a hospital-based blood centre in a tertiary care referral hospital: A perspective from a lower middle-income country.","authors":"Aparna Krishna, Hem Chandra Pandey, Poonam Coshic, Rakesh Kumar, Romesh Jain","doi":"10.1111/vox.13767","DOIUrl":"10.1111/vox.13767","url":null,"abstract":"<p><strong>Background and objectives: </strong>Wrong blood in tube (WBIT) continues to be a preventable cause of unintended harm to the patient. The literature describing extent of the problem, its consequences and factors leading to WBIT from the perspective of lower middle-income countries (LMICs) is limited. The present study describes WBIT and its outcome in a hospital-based blood centre from an LMIC.</p><p><strong>Materials and methods: </strong>WBIT events occurring during the study period were analysed to identify the root cause. In addition, they were analysed according to discipline, department and time of sample draw. Root causes were divided and compared with standard operating procedure (SOP) for sample collection for blood requests. All WBIT events were followed and their outcomes analysed.</p><p><strong>Results: </strong>WBIT events occurred at a rate of 4.8/10,000 blood requests, with a higher rate in urgent requests (5.2/10,000 requests). The average rate of WBIT was higher in surgical disciplines compared to medical and acute care services (6.58 vs. 4.43 vs. 3/10,000 requests). The highest rate of WBIT was observed when requests were received during 8:00 PM-2:00 AM (p = 0.02). Deviations from SOP with contribution from human and organizational elements were identified as the root cause. The consequences ranged from delay in providing blood to acute haemolytic transfusion reactions.</p><p><strong>Conclusion: </strong>We found that WBITs occurred at a rate comparable to that reported from developed countries. Use of software and automation may reduce the rate of WBIT but not eliminate it completely. Strict adherence to SOPs and continuous training of phlebotomy staff would help reduce it to a minimum. Blood centres need to develop specific strategies with respect to their root causes.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"155-162"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and efficacy of a novel mini-pool intravenous immunoglobulin therapy in children with primary immunodeficiency.","authors":"Alshaimaa M Selim, Taghreed M Kamal, Madeen Adel A Abdou, Eman NasrEldin, Nada O Abdelhameed, Mariam E Abdallah, Naglaa S Osman, Maha Atwa, Magdy El-Ekiaby","doi":"10.1111/vox.13769","DOIUrl":"10.1111/vox.13769","url":null,"abstract":"<p><strong>Background and objectives: </strong>Intravenous polyvalent immunoglobulins (IVIG) for prophylaxis in patients with primary immunodeficiency disorders (PIDs) exposes them to life-threatening infections and debilitating diseases. To improve access to IVIG in lower middle-income countries, the WHO recommends a stepwise approach for the local production of purified and virus-inactivated plasma immunoglobulins by national blood transfusion services using new technologies and medical devices. One new technology relies on single-use sterile medical devices for the purification of plasma immunoglobulin G (IgG), as well as lipid-enveloped virus inactivation from mini-pools of recovered plasma separated from whole blood (mini-pool IVIG [MP-IVIG]). This study aimed to compare the safety and efficacy of MP-IVIG to standard IVIG (STD-IVIG).</p><p><strong>Materials and methods: </strong>In this prospective crossover clinical study, we investigated the safety and efficacy of MP-IVIG for STD-IVIG preparations as a replacement therapy in a cohort of 21 paediatric patients with PID.</p><p><strong>Results: </strong>Both MP-IVIG and STD-IVIG were effective in reducing the frequency of severe bacterial infections and hospital admissions in patients with PID. Mild side effects have been observed in seven patients (6.2%) with PID who received MP-IVIG and five patients (5.3%) who received STD-IVIG. No moderate or severe side effects or haemolytic transfusion reactions were reported. The mortality rates were also comparable and were not related to the study products.</p><p><strong>Conclusion: </strong>MP-IVIG presented no safety issues and was as effective as STD-IVIG in IgG replacement in patients with PID. Due to the small numbers, the results have to be addressed with caution.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"140-148"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Forum on Donor- and Recipient-triggered Lookback for Traditional Transfusion-transmitted Infections: Summary.","authors":"Ryanne Lieshout-Krikke, Veronica Hoad, Sze Sze Chua, Grace Kam, Masahiro Satake, Ikuo Hino, Susan L Stramer, Jamel A Groves, Virginie de La Taille, Syria Laperche, Anthea Cheng, Kathryn Goodison, Wai-Chiu Tsoi, Cheuk-Kwong Lee, Daniele Prati, Ilaria Pati, Steven J Drews, Mark Bigham, Georg Gratz, Christof Jungbauer, Richard Charlewood, Meredith Smith, Niamh O'Flaherty, Aoife Raftery, Salvador Oyonarte, Knut Gubbe, Juergen Luhm, Solomuzi Ngcobo, Ed Slot, Katy Davison, Su Brailsford, Nancy Dunbar","doi":"10.1111/vox.13763","DOIUrl":"10.1111/vox.13763","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"197-206"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Forum on Donor- and Recipient-triggered Lookback for Traditional Transfusion-transmitted Infections: Responses.","authors":"Ryanne Lieshout-Krikke, Veronica Hoad, Sze Sze Chua, Grace Kam, Masahiro Satake, Ikuo Hino, Susan L Stramer, Jamel A Groves, Virginie de La Taille, Syria Laperche, Anthea Cheng, Kathryn Goodison, Wai-Chiu Tsoi, Cheuk-Kwong Lee, Daniele Prati, Ilaria Pati, Steven J Drews, Mark Bigham, Georg Gratz, Christof Jungbauer, Richard Charlewood, Meredith Smith, Niamh O' Flaherty, Aoife Raftery, Salvador Oyonarte, Knut Gubbe, Juergen Luhm, Solomuzi Ngcobo, Ed Slot, Katy Davison, Su Brailsford, Nancy Dunbar","doi":"10.1111/vox.13764","DOIUrl":"10.1111/vox.13764","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"207-238"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consistent supply of global plasma for global patients.","authors":"Michelle Fransen, Toby Simon, James Knowles, Joshua Penrod","doi":"10.1111/vox.13771","DOIUrl":"10.1111/vox.13771","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"239-240"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-world performance of a clinical droplet digital polymerase chain reaction assay for non-invasive foetal blood group and platelet antigen genotyping of alloimmunized pregnant women with antibodies directed against RhD, RhE, Rhc, RhC, K1, HPA-1a or HPA-5b: A 1-year experience.","authors":"Camilla Calandrini, Onno J H M Verhagen, Ahmed Tissoudali, Christa H E Homburg, Jessica Vessies, Mark Brussee, Erik H van Beers, C Ellen van der Schoot, Masja de Haas","doi":"10.1111/vox.13777","DOIUrl":"10.1111/vox.13777","url":null,"abstract":"<p><strong>Background and objectives: </strong>To test the performance of a new droplet digital polymerase chain reaction (ddPCR) non-invasive foetal blood group and platelet antigen genotyping assay in the setting of a Dutch reference laboratory for foetal blood group and platelet antigen genotyping. Our population comprised 229 consecutive alloimmunized pregnant women who presented between April 2022 and March 2023 with 250 requests for non-invasive foetal RHD, RHE, RHc, RHC, K1, HPA-1a or HPA-5b blood group and platelet antigen genotyping.</p><p><strong>Materials and methods: </strong>Samples were genotyped for blood group and platelet antigen alleles along with methylated RASSF1a (mRASSF1a) and sex-determining region of Y (SRY) and DYS14 as positive foetal controls. Negative blood group and platelet antigen results were issued only when foetal controls were positive; otherwise, such samples were classified as inconclusive.</p><p><strong>Results: </strong>The assay achieved a success rate of 98.4% (246 of 250) because one case was lost to follow-up, one case was solved with quantitative polymerase chain reaction (qPCR) and one case precluded foetal typing due to RHD variant mothers. Only 10 cases needed a second sample and one case a third for a valid final result. We identified 116 maternal-foetal blood group and platelet antigen incompatibilities.</p><p><strong>Conclusion: </strong>Clinical non-invasive foetal blood group and platelet antigen typing of alloimmunized pregnant women via ddPCR is successful and represents an improvement over qPCR because of the addition of a foetal control and because ddPCR circumvents potential interference from maternal cell-free DNA (cfDNA) background for foetal HPA-1 and K1.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"170-177"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}