Vox Sanguinis最新文献_第9页

Is it useful to wash stored red blood cells in cardiopulmonary bypass priming fluid for neonatal cardiac surgery? A single-centre retrospective study. 在新生儿心脏手术心肺旁路引流液中洗涤储存的红细胞是否有用？一项单中心回顾性研究。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2024-10-01 Epub Date: 2024-09-02 DOI: 10.1111/vox.13716

He Wang, Yu Jin, Peng Gao, Jia Liu, Wenting Wang, Peiyao Zhang, Jinping Liu

{"title":"Is it useful to wash stored red blood cells in cardiopulmonary bypass priming fluid for neonatal cardiac surgery? A single-centre retrospective study.","authors":"He Wang, Yu Jin, Peng Gao, Jia Liu, Wenting Wang, Peiyao Zhang, Jinping Liu","doi":"10.1111/vox.13716","DOIUrl":"10.1111/vox.13716","url":null,"abstract":"Background and objectives: Neonatal cardiac surgery requires careful consideration of cardiopulmonary bypass (CPB) priming fluid composition due to small blood volume and immature physiology. This study investigated the impact of allogeneic stored red blood cells (RBCs) processed using an autotransfusion system in CPB priming fluid for neonates.Materials and methods: We compared perioperative parameters, inflammatory mediators, coagulation indicators, vasoactive-inotropic score (VIS) and clinical outcomes between neonates receiving unwashed (n = 56) and washed (n = 45) RBCs in CPB priming fluid. Regression models were used to assess the independent association between RBC washing and patient outcomes.Results: The autotransfusion system improved stored RBC quality. The washed group showed higher peak haematocrit (p < 0.01) and haemoglobin levels (p = 0.04) during CPB, an increased oxygen delivery index during rewarming (p < 0.05) and lower postoperative lactate levels and VIS (p < 0.05). Inflammatory (IL-6, IL-8 and IL-10) and coagulation parameters (D-dimer, fibrinogen and fibrin degradation product) fluctuated compared with baseline but did not significantly differ between groups. The washed group had a lower incidence of hyperlactacidaemia and delayed sternal closure at CPB weaning.Conclusions: Adding washed allogeneic stored RBCs to neonatal CPB priming fluid reduced postoperative lactate elevation and VIS without early improvement in the inflammatory and coagulation systems.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"1072-1081"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose-dependent inactivation of Plasmodium falciparum in red blood cell concentrates by treatment with short-wavelength ultraviolet light. 用短波紫外线处理红细胞浓缩液中的恶性疟原虫的剂量依赖性灭活。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2024-10-01 Epub Date: 2024-07-24 DOI: 10.1111/vox.13714

Swantje Fischer, Susann Zilkenat, Mona Rosse, Torsten J Schulze, Axel Seltsam, Wiebke Handke, Bernd Lepenies, Ute Gravemann

{"title":"Dose-dependent inactivation of Plasmodium falciparum in red blood cell concentrates by treatment with short-wavelength ultraviolet light.","authors":"Swantje Fischer, Susann Zilkenat, Mona Rosse, Torsten J Schulze, Axel Seltsam, Wiebke Handke, Bernd Lepenies, Ute Gravemann","doi":"10.1111/vox.13714","DOIUrl":"10.1111/vox.13714","url":null,"abstract":"Background and objectives: Plasmodium species are naturally transmitted by Anopheles mosquitos. The parasite infects red blood cells (RBCs) and can be transfused with blood products. In non-endemic areas, the main risk of infection arises from travellers coming back and people immigrating from malaria-endemic regions. Endemic countries face a permanent risk of infection from transfusion-transmitted malaria (TTM). TTM may cause life-threatening complications in patients dependent on blood donations. This study aimed to investigate the efficacy of Plasmodium falciparum inactivation in RBC units by treatment with short-wavelength ultraviolet C (UVC) light in the absence of photochemical additives.Materials and methods: RBC units were spiked with P. falciparum to a parasite density of 0.1%-1% and irradiated with up to 4.5 J/cm2 UVC. The parasite density of UVC-treated dilution series and untreated controls were compared over 3 weeks after irradiation.Results: The lowest dose of 1.5 J/cm2 UVC led to a 3.1 log reduction in parasite load compared with the untreated control. The inactivation capacity was dose-dependent. Strikingly, 4.5 J/cm2 led to ≥5.3 log unit reduction, which was equivalent to a complete inactivation in two out of three experiments.Conclusion: Pathogen reduction with UVC light was previously shown to be effective for different bacteria and viruses, but the inactivation of parasites in RBC concentrates was not addressed until now. The present study provides evidence for significant inactivation of P. falciparum-infected RBCs by UVC light.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"1082-1089"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoantibodies to ADAMTS13 in human immunodeficiency virus‐associated thrombotic thrombocytopenic purpura 人类免疫缺陷病毒相关血栓性血小板减少性紫癜中的 ADAMTS13 自身抗体

IF 2.7 4区医学

Vox Sanguinis Pub Date : 2024-09-19 DOI: 10.1111/vox.13738

Muriel Meiring, Mmakgabu Khemisi, Susan Louw, Palanisamy Krishnan

{"title":"Autoantibodies to ADAMTS13 in human immunodeficiency virus‐associated thrombotic thrombocytopenic purpura","authors":"Muriel Meiring, Mmakgabu Khemisi, Susan Louw, Palanisamy Krishnan","doi":"10.1111/vox.13738","DOIUrl":"https://doi.org/10.1111/vox.13738","url":null,"abstract":"Background and ObjectivesThrombotic thrombocytopenic purpura (TTP) is a potentially fatal thrombotic microangiopathic disorder that can result from human immunodeficiency virus (HIV) infection. The pathogenesis involves a deficiency of the von Willebrand factor (vWF) cleaving protease ADAMTS13 (a disintegrin and metalloprotease with thrombospondin motifs member 13) and the presence of anti‐ADAMTS13 autoantibodies. However, there is insufficient information regarding the epitope specificity and reactivity of these autoantibodies. This study aimed to perform epitope‐mapping analysis to provide novel insights into the specific epitopes on ADAMTS13 domains affected by autoantibodies.Materials and MethodsThe study analysed 59 frozen citrate plasma samples from HIV‐associated TTP patients in South Africa, measuring ADAMTS13 activity using Technozyme® ADAMTS13 activity test, total immunoglobulin (Ig) M and IgA antibodies levels using ELISA kit and purifying IgG antibodies using NAb™ Protein G spin columns. A synthetic ADAMTS13 peptide library was used for epitope mapping.ResultsOverall, 90% of samples showed anti‐ADAMTS13 IgG autoantibodies, with 64% of these antibodies being inhibitory, as revealed by mixing studies. Samples with ADAMTS13 antigen levels below 5% showed high anti‐ADAMTS13 IgG autoantibody titres (≥50 IU/mL), whereas those with 5%–10% levels had low autoantibody titres (<50 IU/mL).The metalloprotease, cysteine‐rich and spacer domains were 100% involved in binding anti‐ADAMTS13 IgG antibodies, with 58% of samples containing antibodies binding to the C‐terminal part of the ADAMTS13 disintegrin‐like domain, indicating different pathogenic mechanisms.ConclusionThe metalloprotease, cysteine‐rich and spacer domains are the primary targets for anti‐ADAMTS13 IgG autoantibodies in patients with HIV‐associated TTP. These findings suggest potential effects on the proteolytic activity of ADAMTS13, highlighting the complex nature of the pathogenic mechanisms involved.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":"199 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142249877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extending the post‐thaw shelf‐life of cryoprecipitate when stored at refrigerated temperatures 在冷藏条件下储存低温沉淀可延长其解冻后的保质期

IF 2.7 4区医学

Vox Sanguinis Pub Date : 2024-09-19 DOI: 10.1111/vox.13736

Kelly M. Winter, Rachel G. Webb, Eugenia Mazur, Peta M. Dennington, Denese C. Marks

{"title":"Extending the post‐thaw shelf‐life of cryoprecipitate when stored at refrigerated temperatures","authors":"Kelly M. Winter, Rachel G. Webb, Eugenia Mazur, Peta M. Dennington, Denese C. Marks","doi":"10.1111/vox.13736","DOIUrl":"https://doi.org/10.1111/vox.13736","url":null,"abstract":"Background and ObjectivesThe post‐thaw shelf‐life of cryoprecipitate is 6 h, leading to high wastage. Storage of thawed cryoprecipitate at refrigerated temperatures may be feasible to extend the shelf‐life. This study aimed to evaluate the quality of thawed cryoprecipitate stored at 1–6°C for up to 14 days.Materials and MethodsCryoprecipitate (mini‐ and full‐size packs derived from both apheresis and whole blood [WB] collections) was thawed, immediately sampled and then stored at 1–6°C for up to 14 days. Mini‐packs were sampled at 6, 24, 48 and 72 h, day 7 and 14; full‐size cryoprecipitate was sampled on day 3, 5 or 7. Coagulation factors (F) II, V, VIII, IX, X and XIII, von Willebrand factor (VWF) and fibrinogen were measured using a coagulation analyser. Thrombin generation was measured by calibrated automated thrombogram.ResultsFVIII decreased during post‐thaw storage; this was significant after 24 h for WB (p = 0.0002) and apheresis (p < 0.0001). All apheresis and eight of 20 WB cryoprecipitate met the FVIII specification (≥ 70 IU/unit) on day 14 post‐thaw. Fibrinogen remained stable for 48 h, and components met the specification on day 14 post‐thaw. There were no significant differences in VWF (WB p = 0.1292; apheresis p = 0.1507) throughout storage. There were small but significant decreases in thrombin generation lag time, endogenous thrombin potential and time to peak for both WB and apheresis cryoprecipitate.ConclusionWhilst coagulation factors in cryoprecipitate decreased after post‐thaw storage, the thawed cryoprecipitate met the Council of Europe specifications when stored at refrigerated temperatures for 7 days.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":"17 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142249878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to ‘Determining the impact of current Canadian stem cell registry policy on donor availability via dynamic registry simulation’ 通过动态登记模拟确定加拿大干细胞登记处现行政策对捐献者可用性的影响 "的更正

IF 2.7 4区医学

Vox Sanguinis Pub Date : 2024-09-15 DOI: 10.1111/vox.13737

引用次数: 0

Events 活动

IF 2.7 4区医学

Vox Sanguinis Pub Date : 2024-09-11 DOI: 10.1111/vox.13733

引用次数: 0

The prevalence of hepatitis B virus, human T‐lymphotropic virus and human immunodeficiency virus in patients receiving blood transfusions in South Africa 南非输血患者中乙型肝炎病毒、人类 T 淋巴细胞病毒和人体免疫缺陷病毒的流行情况

IF 2.7 4区医学

Vox Sanguinis Pub Date : 2024-09-11 DOI: 10.1111/vox.13735

Reynier J. Willemse, Christa J. Grobler, Edward L. Murphy, Nareg Roubinian, Charl Colemen, Solly Machaba, Marion Vermeulen

{"title":"The prevalence of hepatitis B virus, human T‐lymphotropic virus and human immunodeficiency virus in patients receiving blood transfusions in South Africa","authors":"Reynier J. Willemse, Christa J. Grobler, Edward L. Murphy, Nareg Roubinian, Charl Colemen, Solly Machaba, Marion Vermeulen","doi":"10.1111/vox.13735","DOIUrl":"https://doi.org/10.1111/vox.13735","url":null,"abstract":"Background and ObjectivesSouth Africa has a high prevalence of human immunodeficiency virus (HIV) and hepatitis B virus (HBV) and to a lesser extent human T‐lymphotropic virus (HTLV). Each of these agents is transfusion‐transmissible (TT) but deciding whether to implement preventive screening depends upon knowledge of background prevalence in transfused patients. We determined the prevalence of HIV, HBV and HTLV I/II among blood transfusion recipients in South African hospitals.Materials and MethodsWe obtained identity‐unlinked samples used for blood cross‐matching at 634 South African hospitals served by the South African National Blood Service (SANBS). The ABBOTT Alinity S® Immunochemiluminescent system measured HIV, HBV and HTLV I/II antibodies. Repeatedly reactive samples were confirmed using the Roche Cobas® 8000. Logistic regression was performed to investigate the determinants of associations for HIV, HBV and HTLV infections.ResultsThe overall prevalences of HIV, HBV and HTLV were 37.8%, 7.4% and 0.6%, respectively. The HIV prevalence in blood recipients was twice as high as general population estimates. Public hospital patients had a significantly higher prevalence compared with private hospital patients for HIV and HBV. HIV prevalence was significantly higher in females, and HBV prevalence was significantly higher in males, excluding the unknown gender results.ConclusionPatients receiving blood transfusions in South Africa have high rates of HIV and HBV infection that should be taken into consideration when determining donor screening strategies for other viral infections. Measurable prevalence of HTLV indicates endemicity of this infection in South Africa.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":"43 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive approach to continuous quality improvement of massive transfusion by developing key performance indicators 通过制定关键绩效指标持续改进大规模输血质量的综合方法

IF 2.7 4区医学

Vox Sanguinis Pub Date : 2024-09-10 DOI: 10.1111/vox.13732

Ancy Ninan, Vimal Krishnan, Shamee Shastry, Ganesh Mohan, Deepika Chenna, Deep Madkaiker, Jayaraj Mymbilly Balakrishnan

{"title":"A comprehensive approach to continuous quality improvement of massive transfusion by developing key performance indicators","authors":"Ancy Ninan, Vimal Krishnan, Shamee Shastry, Ganesh Mohan, Deepika Chenna, Deep Madkaiker, Jayaraj Mymbilly Balakrishnan","doi":"10.1111/vox.13732","DOIUrl":"https://doi.org/10.1111/vox.13732","url":null,"abstract":"Background and ObjectivesTo develop key performance indicators (KPI) for use in quality assessment of our institutional goal‐directed massive transfusion (GDMT).Materials and MethodsA team comprising our transfusion and emergency medicine departments carried out a cross‐sectional data analysis of GDMT in adult patients from January 2021 to December 2022. The study was rooted in the Define, Measure, Analyse, Improve, Control (DMAIC) approach. Features of KPIs were (a) importance, (b) scientific soundness and (c) feasibility. Study parameters were defined and analysed using measures of central tendencies and benchmark comparison.ResultsNinety‐two massive transfusion events occurred and 1405 blood components were used. Trauma was the leading cause, followed by postpartum haemorrhage and upper gastrointestinal bleeding. Appropriate GDMT activation was observed only in 43.47% of events. The turnaround time (TAT) was within the benchmark in 85.8% of events with an average of 16 ± 10 min. The average utilization of blood components was 20.5 (interquartile range [IQR] = 11.3) in the appropriate group and 5.5 (IQR = 4.25) in the inappropriate group with a wastage rate of 3.5%. Duration of activation was 6.19 ± 4.59 h, and the adherence to thromboelastography was 66.3%. Overall mortality was 45.65%, and the average duration of hospital stay was 6.1 ± 5.9 days.ConclusionThe KPIs developed were easy to capture, and the analysis provided a comprehensive approach to the quality improvement of the GDMT protocol.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":"18 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New tatt? We're ok with that! Relaxing the tattoo deferral for plasmapheresis donors maintains safety and increases donations. 新纹身？我们可以接受！放宽血浆置换捐献者纹身推迟期的规定可确保安全并增加捐献。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2024-09-01 Epub Date: 2024-06-26 DOI: 10.1111/vox.13704

Claire E Styles, Veronica C Hoad, Robert Harley, John Kaldor, Iain B Gosbell

{"title":"New tatt? We're ok with that! Relaxing the tattoo deferral for plasmapheresis donors maintains safety and increases donations.","authors":"Claire E Styles, Veronica C Hoad, Robert Harley, John Kaldor, Iain B Gosbell","doi":"10.1111/vox.13704","DOIUrl":"10.1111/vox.13704","url":null,"abstract":"Background and objectives: Tattooing is one of the leading donor deferral reasons in Australia. Until September 2020, donors were deferred from all donation types for 4 months after a tattoo. At this time, our guideline changed such that donations of plasma for further manufacture were accepted immediately, provided the tattoo was administered in a licensed or regulated Australian establishment. We examined the effects of this change.Materials and methods: Donors with a tattoo deferral in the 2 years before or after the guideline change were identified and followed up until 3 November 2022. Between the two periods, we compared blood-borne virus (BBV) incidence, donor return, and the number of donors and donations regained after deferral.Results: The incidence of BBV infection in donors after a tattoo deferral was zero in both periods. To exceed a residual risk of 1 in 1 million for hepatitis C virus, 190 donors would need to be infected yearly from a tattoo. Donors returned to donate significantly faster after the change (median return 85 days compared with 278 days). An extra 187 donations per 10,000 person-years of observation were gained, yielding a total of 44,674 additional plasma donations nationally 0-4 months after getting a tattoo.Conclusion: Allowing plasma donations immediately post-tattoo resulted in a substantial donation gain with no adverse safety effect. Lifeblood subsequently reduced the deferral for transfusible component donations to 7 days for tattoos in Australian licensed/regulated establishments.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"927-935"},"PeriodicalIF":1.8,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond PVC-The coming storm? 超越 PVC-暴风雨即将来临？

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2024-09-01 Epub Date: 2024-06-03 DOI: 10.1111/vox.13689

Gillian Grafton, Stephen Thomas

引用次数: 0