分裂的红细胞单位含有确定的细胞外K+水平，通过洗涤程序提高。

IF 1.8 4区医学 Q3 HEMATOLOGY

Vox Sanguinis Pub Date : 2025-02-17 DOI:10.1111/vox.70004

Fabiola Hoppe, Jacqueline Maier, Holger Kirsten, Martin Federbusch, Reinhard Henschler

{"title":"分裂的红细胞单位含有确定的细胞外K+水平，通过洗涤程序提高。","authors":"Fabiola Hoppe, Jacqueline Maier, Holger Kirsten, Martin Federbusch, Reinhard Henschler","doi":"10.1111/vox.70004","DOIUrl":null,"url":null,"abstract":"Background and objectives: We should control free K+ during massive transfusion (>80 mL/kg) of red blood cells (RBCs) in small children. To manage this, several national and international guidelines recommend using RBCs stored only up to 7 days. We tested a washing step for RBCs in saline-adenine-glucose-mannitol (SAGM) with or without irradiation to reduce supernatant K+ levels, improve quality and potentially extend the shelf life of stored RBCs.Materials and methods: RBCs of 240-330 mL were prepared from whole blood donations, then split into halves and stored in SAGM solution at 4 ± 2°C for 21 days. RBCs were split and washed on Days 1 and 8, and some were gamma-irradiated on Day 8. Glucose, lactate, lactate dehydrogenase (LDH), adenosine triphosphate (ATP), K+ and haemolysis were determined over 21 days.Results: After washing on Day 1, only glucose and lactate improved, whereas after washing on Day 8, LDH and K+ also improved. Irradiation resulted in accelerated K+ accumulation and increased haemolysis. Mean extracellular K+ concentrations were 21.2 ± 1.03 mM after irradiation on Day 8 versus 1.12 ± 0.05 mM after irradiation plus wash on Day 8, and 38.80 ± 2.13 mM on Day 10 after irradiation on Day 8 and 16.6 ± 0.05 mM on Day 10 after irradiation plus wash on Day 8.Conclusion: K+ concentrations remained <25 mM within 8 days of storage. We recommend irradiation by Day 8 at the latest for neonatal transfusion. The shelf life may be extended by another 48 h if the RBCs are also washed.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Split red blood cell units contain defined extracellular K+ levels, which are improved by a washing procedure.\",\"authors\":\"Fabiola Hoppe, Jacqueline Maier, Holger Kirsten, Martin Federbusch, Reinhard Henschler\",\"doi\":\"10.1111/vox.70004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background and objectives: We should control free K+ during massive transfusion (>80 mL/kg) of red blood cells (RBCs) in small children. To manage this, several national and international guidelines recommend using RBCs stored only up to 7 days. We tested a washing step for RBCs in saline-adenine-glucose-mannitol (SAGM) with or without irradiation to reduce supernatant K+ levels, improve quality and potentially extend the shelf life of stored RBCs.Materials and methods: RBCs of 240-330 mL were prepared from whole blood donations, then split into halves and stored in SAGM solution at 4 ± 2°C for 21 days. RBCs were split and washed on Days 1 and 8, and some were gamma-irradiated on Day 8. Glucose, lactate, lactate dehydrogenase (LDH), adenosine triphosphate (ATP), K+ and haemolysis were determined over 21 days.Results: After washing on Day 1, only glucose and lactate improved, whereas after washing on Day 8, LDH and K+ also improved. Irradiation resulted in accelerated K+ accumulation and increased haemolysis. Mean extracellular K+ concentrations were 21.2 ± 1.03 mM after irradiation on Day 8 versus 1.12 ± 0.05 mM after irradiation plus wash on Day 8, and 38.80 ± 2.13 mM on Day 10 after irradiation on Day 8 and 16.6 ± 0.05 mM on Day 10 after irradiation plus wash on Day 8.Conclusion: K+ concentrations remained <25 mM within 8 days of storage. We recommend irradiation by Day 8 at the latest for neonatal transfusion. The shelf life may be extended by another 48 h if the RBCs are also washed.\",\"PeriodicalId\":23631,\"journal\":{\"name\":\"Vox Sanguinis\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-02-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vox Sanguinis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/vox.70004\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vox Sanguinis","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/vox.70004","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

背景和目的：在儿童大量输血（红细胞）时，应控制游离K+ (80 mL/kg)。为了解决这一问题，一些国家和国际指南建议使用储存最多7天的红细胞。我们测试了在盐水-腺嘌呤-葡萄糖-甘露醇（SAGM）中对红细胞进行清洗的步骤，有或没有照射，以降低上清K+水平，提高质量，并可能延长储存红细胞的保质期。材料和方法：献血全血制备红细胞240 ~ 330ml，切成两半，在4±2℃的SAGM溶液中保存21天。红细胞在第1天和第8天分裂和清洗，部分红细胞在第8天进行γ照射。21 d内测定葡萄糖、乳酸、乳酸脱氢酶（LDH）、三磷酸腺苷（ATP）、钾离子（K+）和溶血。结果：第1天洗涤后，只有葡萄糖和乳酸有所改善，第8天洗涤后，LDH和K+也有所改善。照射导致K+积累加速和溶血增加。第8天照射后细胞外K+平均浓度分别为21.2±1.03 mM和1.12±0.05 mM，第8天照射后10天为38.80±2.13 mM，第8天照射后10天为16.6±0.05 mM。结论：K+浓度保持不变

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Split red blood cell units contain defined extracellular K⁺ levels, which are improved by a washing procedure.

Background and objectives: We should control free K⁺ during massive transfusion (>80 mL/kg) of red blood cells (RBCs) in small children. To manage this, several national and international guidelines recommend using RBCs stored only up to 7 days. We tested a washing step for RBCs in saline-adenine-glucose-mannitol (SAGM) with or without irradiation to reduce supernatant K⁺ levels, improve quality and potentially extend the shelf life of stored RBCs.

Materials and methods: RBCs of 240-330 mL were prepared from whole blood donations, then split into halves and stored in SAGM solution at 4 ± 2°C for 21 days. RBCs were split and washed on Days 1 and 8, and some were gamma-irradiated on Day 8. Glucose, lactate, lactate dehydrogenase (LDH), adenosine triphosphate (ATP), K⁺ and haemolysis were determined over 21 days.

Results: After washing on Day 1, only glucose and lactate improved, whereas after washing on Day 8, LDH and K⁺ also improved. Irradiation resulted in accelerated K⁺ accumulation and increased haemolysis. Mean extracellular K⁺ concentrations were 21.2 ± 1.03 mM after irradiation on Day 8 versus 1.12 ± 0.05 mM after irradiation plus wash on Day 8, and 38.80 ± 2.13 mM on Day 10 after irradiation on Day 8 and 16.6 ± 0.05 mM on Day 10 after irradiation plus wash on Day 8.

Conclusion: K⁺ concentrations remained <25 mM within 8 days of storage. We recommend irradiation by Day 8 at the latest for neonatal transfusion. The shelf life may be extended by another 48 h if the RBCs are also washed.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Vox Sanguinis 医学-血液学

CiteScore

4.40

自引率

11.10%

发文量

156

审稿时长

6-12 weeks

期刊介绍： Vox Sanguinis reports on important, novel developments in transfusion medicine. Original papers, reviews and international fora are published on all aspects of blood transfusion and tissue transplantation, comprising five main sections: 1) Transfusion - Transmitted Disease and its Prevention: Identification and epidemiology of infectious agents transmissible by blood; Bacterial contamination of blood components; Donor recruitment and selection methods; Pathogen inactivation. 2) Blood Component Collection and Production: Blood collection methods and devices (including apheresis); Plasma fractionation techniques and plasma derivatives; Preparation of labile blood components; Inventory management; Hematopoietic progenitor cell collection and storage; Collection and storage of tissues; Quality management and good manufacturing practice; Automation and information technology. 3) Transfusion Medicine and New Therapies: Transfusion thresholds and audits; Haemovigilance; Clinical trials regarding appropriate haemotherapy; Non-infectious adverse affects of transfusion; Therapeutic apheresis; Support of transplant patients; Gene therapy and immunotherapy. 4) Immunohaematology and Immunogenetics: Autoimmunity in haematology; Alloimmunity of blood; Pre-transfusion testing; Immunodiagnostics; Immunobiology; Complement in immunohaematology; Blood typing reagents; Genetic markers of blood cells and serum proteins: polymorphisms and function; Genetic markers and disease; Parentage testing and forensic immunohaematology. 5) Cellular Therapy: Cell-based therapies; Stem cell sources; Stem cell processing and storage; Stem cell products; Stem cell plasticity; Regenerative medicine with cells; Cellular immunotherapy; Molecular therapy; Gene therapy.