Vox Sanguinis最新文献

{"title":"Plasma exchange in Susac syndrome: Unlocking treatment for a rare disease.","authors":"Yamac Akgun","doi":"10.1111/vox.70017","DOIUrl":"https://doi.org/10.1111/vox.70017","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet transfusion stated practices among neonatal and paediatric veno-arterial extracorporeal membrane oxygenation providers: A survey.","authors":"Trupti Ingle, Brian Simms, Jesse Bain, Melania M Bembea, Jill M Cholette, Madhuradhar Chegondi, Eva Cheung, Robert Niebler, Caroline Ozment, Matthew Paden, Ofer Schiller, Marianne E Nellis, Oliver Karam","doi":"10.1111/vox.70018","DOIUrl":"https://doi.org/10.1111/vox.70018","url":null,"abstract":"<p><strong>Background and objectives: </strong>Extracorporeal membrane oxygenation (ECMO) provides cardiopulmonary support to over 4000 neonates and children annually worldwide. Although potentially lifesaving, ECMO carries bleeding and thromboembolic risks, often managed with platelet transfusions to maintain specific thresholds. Platelet transfusions themselves carry many risks. This survey aimed to describe stated prophylactic platelet transfusion practices among paediatric veno-arterial (VA)-ECMO providers and identify factors influencing transfusion decisions.</p><p><strong>Materials and methods: </strong>This is a cross-sectional electronic survey of paediatric ECMO providers from 10 centres evaluating platelet transfusion thresholds based on six patient scenarios (non-bleeding, minimally bleeding and resolved bleeding in neonates and children). Descriptive statistics were used for analysis.</p><p><strong>Results: </strong>The survey response rate was 56% (114 of 204). Paediatric intensivists comprised 66% of respondents. The median pre-transfusion platelet count varied across VA-ECMO scenarios, with a threshold of 50 × 10⁹/L (interquartile range [IQR] 45-75) for non-bleeding children and 70 × 10⁹/L (IQR 50-85) for non-bleeding neonates. The threshold for minimally bleeding children, minimally bleeding neonates and resolved bleeding in children was 75 × 10⁹/L (IQR 50-100). The threshold for resolved bleeding in neonates was 80 × 10⁹/L (IQR 50-100). There was significant heterogeneity between and within sites (p < 0.001). Uncertainty about the level of evidence was high (59%), with clinical judgement being the most influential factor in transfusion decisions (85%).</p><p><strong>Conclusion: </strong>Prophylactic platelet transfusion practices in paediatric ECMO vary widely, highlighting uncertainty and the need for clinical trials to improve patient outcomes.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of an A_weakB phenotype caused by significant ABO gene deletion in a Chinese woman.","authors":"Tian Gao, Feiyu Jiang, Shoufang Xu, Yingying Zhang, Weiwei Zheng, Xinhui Wang, Zhiwei Liu","doi":"10.1111/vox.70008","DOIUrl":"https://doi.org/10.1111/vox.70008","url":null,"abstract":"<p><strong>Background and objectives: </strong>The A_weakB is a rare phenotype in the ABO blood group system. Here, we first report a novel ABO mutation discovered in a Chinese woman with an A_weakB. Third-generation sequencing was employed to investigate the molecular mechanisms underlying A_weakB. By correctly identifying the phenotype, it was useful for increasing the safety of blood transfusion.</p><p><strong>Materials and methods: </strong>ABO blood group was identified by the standard ABO serological test and polymerase chain reaction with sequence-specific primers (PCR-SSP). To analyse the ABO gene sequence, single-molecule real-time (SMRT) sequencing was performed to obtain full-length sequencing of the ABO gene.</p><p><strong>Results: </strong>The Chinese individual was serologically identified as A_weakB subtype, and SMRT sequencing analysis revealed that there were large fragment deletion mutations in the promoter (c.1-1326_1-1321del, c.1-1010_1-975del, c.1-952_1-1del) and Exon 1 region (c.1_28del).</p><p><strong>Conclusion: </strong>We report for the first time that large fragment deletions represent a novel molecular basis for the A_weakB. These deletions may potentially influence the expression of the A antigen.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of instant messaging applications in Indian blood banking.","authors":"Satyam Arora, Kriti Batni, Seema Dua, Anupa Pokhrel","doi":"10.1111/vox.70010","DOIUrl":"https://doi.org/10.1111/vox.70010","url":null,"abstract":"<p><strong>Background and objectives: </strong>Instant messaging applications (IMAs) have revolutionized the means of communication. Like various disciplines in medicine, transfusion medicine and blood banking have also utilized these applications for connecting with donors for recruitment, routine blood centre work and clinical consulting. We aim to study the pattern of usage of IMAs by the Indian blood bank community.</p><p><strong>Materials and methods: </strong>This was a descriptive cross-sectional survey (27 questions) of doctors and technicians working in the blood centres. The target was to collect at least 100 responses from blood bank staff. The survey was open from 28 March 2024 to 28 May 2024.</p><p><strong>Results: </strong>One hundred and thirty-nine out of 575 individuals dealing with blood banking responded to the survey (24% response rate). The responders were mainly doctors (85.6%), young professionals (<40 years; 53.2%) and males (53.9%). WhatsApp® on smartphones seems to be one of the most preferred ways (~95% responders) of IMAs among the Indian blood bank community. IMAs were mainly used to communicate with the blood centre staff (83%) as well as clinical counter colleagues (68.5%). More than 50% of our responders were members of more than five chat groups dealing with blood bank. IMAs were also used to disseminate knowledge, such as sharing recent guidelines (81%) and research articles (69%).</p><p><strong>Conclusion: </strong>Our study demonstrates that the blood bank community has embraced the internet-based IMA (mainly WhatsApp) and uses it mainly on their mobile devices.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence chatbots in transfusion medicine: A cross-sectional study.","authors":"Prateek Srivastava, Ashish Tewari, Arwa Z Al-Riyami","doi":"10.1111/vox.70009","DOIUrl":"https://doi.org/10.1111/vox.70009","url":null,"abstract":"<p><strong>Background and objectives: </strong>The recent rise of artificial intelligence (AI) chatbots has attracted many users worldwide. However, expert evaluation is essential before relying on them for transfusion medicine (TM)-related information. This study aims to evaluate the performance of AI chatbots for accuracy, correctness, completeness and safety.</p><p><strong>Materials and methods: </strong>Six AI chatbots (ChatGPT 4, ChatGPT 4-o, Gemini Advanced, Copilot, Anthropic Claude 3.5 Sonnet, Meta AI) were tested using TM-related prompts at two time points, 30 days apart. Their responses were assessed by four TM experts. Evaluators' scores underwent inter-rater reliability testing. Responses from Day 30 were compared with those from Day 1 to evaluate consistency and potential evolution over time.</p><p><strong>Results: </strong>All six chatbots exhibited some level of inconsistency and varying degrees of evolution in their responses over 30 days. None provided entirely correct, complete or safe answers to all questions. Among the chatbots tested, ChatGPT 4-o and Anthropic Claude 3.5 Sonnet demonstrated the highest accuracy and consistency, while Microsoft Copilot and Google Gemini Advanced showed the greatest evolution in their responses. As a limitation, the 30-day period may be too short for a precise assessment of chatbot evolution.</p><p><strong>Conclusion: </strong>At the time of the conduct of this study, none of the AI chatbots provided fully reliable, complete or safe responses to all TM-related prompts. However, ChatGPT 4-o and Anthropic Claude 3.5 Sonnet show the highest promise for future integration into TM practices. Given their variability and ongoing development, AI chatbots should not yet be relied upon as authoritative sources in TM without expert validation.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of transfusing older red blood cells on patient outcomes in critical illness: A retrospective cohort study.","authors":"Nchafatso G Obonyo, Declan P Sela, Nicole White, Matthew Tunbridge, Beatrice Sim, Reema H Rachakonda, Louise E See Hoe, Gianluigi Li Bassi, Jonathon P Fanning, John-Paul Tung, Jacky Y Suen, John F Fraser","doi":"10.1111/vox.70007","DOIUrl":"https://doi.org/10.1111/vox.70007","url":null,"abstract":"<p><strong>Background and objectives: </strong>Randomized controlled trials have demonstrated morbidity and mortality in critically ill patients are unaffected by transfusing fresh (<7 days old) packed red blood cells (pRBCs); however, there is limited evidence regarding transfusion with pRBCs nearing expiry (35-42 days). The aim of this study was to investigate the effects of transfusing pRBCs close to the end of shelf life (≥35 days) on clinical outcomes in critically ill patients.</p><p><strong>Materials and methods: </strong>A retrospective observational analysis of data obtained from centralized electronic medical records (2007-2013), sourced from all public and licensed private hospitals in Queensland, Australia, with intensive care units. Multivariate logistic and linear regressions were used to analyse association between transfusion with pRBCs nearing expiry, and in-hospital mortality, hospital length of stay (HLOS) and rate of discharge home. Comparisons are presented as odds ratios (ORs) with 95% confidence intervals (CIs).</p><p><strong>Results: </strong>The study included 10,350 critically ill adult patients transfused ≥1 unit of non-irradiated pRBCs (64,594 pRBCs units transfused). Receiving at least 1-unit pRBCs ≥ 35 days old was associated with increased mortality (OR 1.21 [95% CI 1.06-1.38]; p = 0.005), decreased discharge to usual residence (OR 0.81 [95% CI 0.73-0.89]; p < 0.0001) and increased hospital LOS (estimate 2.55 [95% CI 1.60-3.49]; p < 0.0001). There was also association with increased sepsis (OR 1.27 [95% CI 1.13-1.42]; p < 0.0001) and delirium (OR 1.25 [95% CI 1.06-1.49]; p = 0.01).</p><p><strong>Conclusion: </strong>Transfusion of ≥1-unit pRBCs ≥ 35 days old was associated with higher morbidity and mortality in critically ill patients.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a simplified platelet cryopreservation method: An in vitro investigation of reducing the DMSO concentration to allow administration without its pre-transfusion removal.","authors":"Lacey Johnson, Pearl Lei, Christopher Roan, Denese C Marks","doi":"10.1111/vox.13789","DOIUrl":"https://doi.org/10.1111/vox.13789","url":null,"abstract":"<p><strong>Background and objectives: </strong>The most widely used method of platelet cryopreservation requires the addition of 5%-6% dimethylsulphoxide (DMSO), followed by its pre-freeze removal via centrifugation, to minimize toxicity. However, this adds complexity to the pre-freeze and post-thaw processing. Accordingly, the aim of this study was to simplify platelet cryopreservation by reducing the DMSO concentration and omitting the requirement for pre-transfusion removal.</p><p><strong>Materials and methods: </strong>Apheresis platelets were cryopreserved at -80°C according to standard blood-banking methods using 5.5% DMSO, with centrifugation, pre-freeze removal of DMSO and reconstitution in plasma following thawing (standard). In parallel, doses of DMSO (0%, 1.5%, 3%, 5.5%) were tested without centrifugation and reconstitution (no-wash). In vitro platelet quality was assessed by flow cytometry, aggregation, viscoelastic testing (thromboelastography [TEG]) and clot retraction.</p><p><strong>Results: </strong>Many in vitro platelet quality parameters showed DMSO dose dependency using the no-wash protocol (recovery, annexin-V, TEG maximum amplitude [MA]). Platelets frozen using the no-wash method with 3% DMSO showed a higher abundance of GPIbα (3% DMSO no-wash median fluorescence intensity [MFI]: 228 ± 16; standard MFI: 184 ± 16; p = 0.0016) and less degranulation (reduced P-selectin-positive platelets and concentration of supernatant P-selectin) than platelets frozen using the standard method. All functional properties measured were comparable to those of platelets frozen using the standard method.</p><p><strong>Conclusion: </strong>This study shows that improvements in cryopreserved platelet quality parameters can be obtained by removing the centrifugation processes (standard vs. 5.5% DMSO no-wash). A reduction in DMSO to 3% supports quality parameters, and if shown to be clinically acceptable, this cryopreservation method could improve platelet accessibility, as it is simpler and cheaper than the standard method.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":"120 3","pages":"284-292"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forty-eight-hour cold-stored whole blood in paediatric cardiac surgery: Implications for haemostasis and blood donor exposures.","authors":"Amy L Kiskaddon, Jennifer Andrews, Cassandra D Josephson, Michael T Kuntz, Dominique Tran, Jennifer Jones, Vyas Kartha, Nhue L Do","doi":"10.1111/vox.13786","DOIUrl":"10.1111/vox.13786","url":null,"abstract":"<p><strong>Background and objectives: </strong>Cold-stored whole blood (CS-WB) in paediatric cardiac surgery is making a resurgence, given its identified benefits compared to conventional blood component therapy (CT).</p><p><strong>Study design and methods: </strong>A single-centre retrospective study was conducted from January 2018 to October 2018 by including children <18 years of age undergoing cardiac surgery requiring cardiopulmonary bypass. ABO-compatible CS-WB from non-directed random donors was leukoreduced with platelet-sparing filters and compared with CT.</p><p><strong>Results: </strong>Fifty-seven patients (30, 53% CS-WB; 27, 47% CT) were studied. Patient demographics were similar, although CT patients were cooled to a lower intra-operative temperature. Blood product requirements 24 h post operation were less in the CS-WB group (11.1 vs. 26.7 mL/kg, p = 0.048). Twelve (40%) patients in the CS-WB cohort had more than one donor exposure versus 25 (93%) in the CT group (p < 0.001). CT patients compared to CS-WB patients had a greater decrease in pre-operative versus 48-h post-operative haemoglobin, platelets and prothrombin time. Patients who received CT compared to CS-WB had a trend towards higher median (interquartile range [IQR]) chest-tube output (mL/kg/h) in the first 4 h post cardiac intensive care unit (ICU) admission (2.1 [0.8, 3] vs. 1.6 [0.8, 2.2], p = 0.197). There was no difference in antifibrinolytic use, length of stay, sepsis, acute kidney injury or wound infection. Survival to discharge was similar.</p><p><strong>Conclusion: </strong>CS-WB in paediatric cardiac surgery may reduce donor exposure and improve haemostatic balance. Future multi-centre prospective studies are needed to validate these findings and identify patients who would benefit from CS-WB in paediatric cardiac surgery.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"293-300"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engaging with young professionals in transfusion medicine: Insights from a needs assessment survey targeting an international cohort.","authors":"Eunike C McGowan, Antoine Lewin, Jian Ou-Yang, Saikat Mandal, Nour Almozain, Abiy Belay Ambaye, Jannison Karlly Cavalcante Ribeiro, Lilian Antwi Boateng, Carla Luana Dinardo, Praiseldy Langi Sasongko","doi":"10.1111/vox.13783","DOIUrl":"10.1111/vox.13783","url":null,"abstract":"<p><strong>Background and objectives: </strong>Professionals who work or study in transfusion medicine under 40 years of age are considered young professionals (YPs) by the International Society of Blood Transfusion (ISBT). While the ISBT provides opportunities for YPs, their needs have to be assessed to customize initiatives in a way that could potentially improve their engagement. This survey aimed to assess the needs of YPs in transfusion medicine and understand their perspectives on future ISBT initiatives.</p><p><strong>Materials and methods: </strong>Between January and February 2023, a 28-question online survey was accessible through a generalized link across the ISBT network. Skip-logic responses from 352 YPs, including 151 ISBT members and 201 non-members, were analysed. Each question varied in the number of responders and, consequently, the number of responses.</p><p><strong>Results: </strong>Firstly, the most important needs of YPs from the survey were educational opportunities and training programmes, with 70% of respondents indicating for educational content in specific fields of transfusion. Secondly, Transfusion Today published by the ISBT (46.9%) ranked the highest in engagement, while ISBT Academy and Academy funding ranked the lowest (12.8%). ISBT members reported attending ISBT activities or using ISBT resources more often than non-members, although this was not statistically significant. The primary barrier preventing both non-members and ISBT members from engaging in ISBT activities was a lack of awareness.</p><p><strong>Conclusion: </strong>Raising awareness on a regular basis, a customized communication style (e.g., by a representative or different languages) and activities for non-members may be key to improving YP engagement and expanding the ISBT network.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"320-325"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Forum on Genotyping of Blood Antigens in Donors: Responses.","authors":"Jennie Haw, Kelly Holloway, Gwen Clarke, Galit Eliyahu Nuni, Liora Muncher, James Daly, Jacqui Martin, Klaus Rieneck, Morten H Dziegiel, Cinzia Paccapelo, Luisa Ronzoni, Diarmaid O'Donghaile, Helen Ryan, Celina Montemayor Garcia, Cheuk-Kwong Lee, Wai-Chiu Tsoi, Hironori Kobayashi, Hiroyuki Igarashi, Tuulia Palukka, Katri Haimila, Nuria Nogues, Cecilia Gonzalez-Santesteban, Aaron J Gottschalk, Åsa Hellberg, Martin L Olsson, Thierry Peyrard, Ute Jentsch, Ai Leen Ang, Chew Yen Phang, Lorna Wall, Dhana Gounder, Nancy Dunbar","doi":"10.1111/vox.13791","DOIUrl":"10.1111/vox.13791","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"333-348"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}