Vox Sanguinis最新文献_第3页

Assessing reagents and techniques for identifying RhCE variants in routine serological testing. 评估常规血清学检测中识别RhCE变异的试剂和技术。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1111/vox.13792

Tatiane Aparecida de Paula Vendrame, Carine Prisco Arnoni, Giovana Faria Devides, Nayara Moraes Silva, Afonso José Pereira Cortez, Flavia Roche Moreira Latini, Lilian Castilho

{"title":"Assessing reagents and techniques for identifying RhCE variants in routine serological testing.","authors":"Tatiane Aparecida de Paula Vendrame, Carine Prisco Arnoni, Giovana Faria Devides, Nayara Moraes Silva, Afonso José Pereira Cortez, Flavia Roche Moreira Latini, Lilian Castilho","doi":"10.1111/vox.13792","DOIUrl":"10.1111/vox.13792","url":null,"abstract":"Background and objectives: Identifying RhCE variants is essential to prevent alloimmunization and manage complex cases. Unfortunately, these variants are often only detected after antibody formation, as they may go unnoticed in serological tests. This study aimed to assess monoclonal antisera using various methodologies to define the reactivity patterns of some variants by variable expression of RhCE antigens.Materials and methods: Samples were chosen based on atypical reactivity on routine RhCE typing of donors, screening of Afro-descendant donors using tube testing and patient samples with antibodies against their own antigens. All 53 samples were tested using tube, gel and microplate tests with five antisera. Antigen expression was assessed by flow cytometry, and RhCE variants were molecularly classified.Results: Tube test screening of African descent donors proved more effective in identifying a broad range of weak or partial antigens, particularly when using anti-e composed of MS-21, MS-16, MS63 clones and anti-c from the MS8011531019 clone. Automated instrument phenotyping successfully identified samples with RHCE*ceJAL allele, while most other variants were detected as positive (4+), similar to gel test, which intensified most reactions. When comparing methods and antisera for detecting variant e antigens, tube test identified a higher percentage of weak samples (63%-77%) compared with microplate (35%) and gel tests (14%).Conclusion: The results highlight the critical role of tube test in serological routines and the need to select clones capable of identifying RhCE variants. Detecting reduced RhCE antigen expression during routine serological testing can guide further molecular investigations and help prevent Rh alloimmunization.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"301-309"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removing upper age restrictions for returning donors and increasing the new donor upper age: Novel adverse event findings using a comprehensive donor vigilance system in Australia. 取消回国捐献者的年龄上限限制，提高新捐献者的年龄上限：澳大利亚利用全面的捐献者警戒系统发现的新的不良事件。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2024-11-25 DOI: 10.1111/vox.13776

Joanna Speedy, Gabrielle Josling, Veronica C Hoad

{"title":"Removing upper age restrictions for returning donors and increasing the new donor upper age: Novel adverse event findings using a comprehensive donor vigilance system in Australia.","authors":"Joanna Speedy, Gabrielle Josling, Veronica C Hoad","doi":"10.1111/vox.13776","DOIUrl":"10.1111/vox.13776","url":null,"abstract":"Background and objectives: In July 2019, Australia removed the upper age limit for returning donors (previously 80 years) and increased the upper age for new donors from 70 to 75 years. This study assessed the safety of our new policy for our newly eligible upper age donors (NEUADs).Materials and methods: For the period, 14 July 2019 to 30 June 2023, the relative risk (RR) of individual adverse events in NEUADs was compared with younger cohorts.Results: There were 4529 NEUADs who made 8000 donations. The overall rates of vasovagal reactions (VVRs) were significantly lower in the NEUAD cohort. However, compared with younger donors; returning NEUADs had a significantly higher rate of loss of consciousness (RR 2.62; 95% confidence interval [CI]: 1.41-4.86) and new NEUADs had a significantly higher rate of offsite VVRs (RR 1.60; 95% CI: 1.08-2.37). Both new and returning NEUADs had significantly higher rates of VVR requiring outside medical care (RR 2.48; 95% CI: 1.28-4.79 and RR 4.45; 95% CI: 2.00-9.91 respectively).Conclusion: Our findings support historical findings that overall VVR rates decline with age but suggest a higher risk of more serious VVRs.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"315-319"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipaemic plasma: An objective non-invasive photometric method to classify plasma turbidity and its association with red cell haemolysis. 血脂血浆：一种客观的无创光度法分类血浆浊度及其与红细胞溶血的关系。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2024-12-02 DOI: 10.1111/vox.13778

Lara A E de Laleijne-Liefting, Ido J Bontekoe, Johan W Lagerberg, Thomas R L Klei

{"title":"Lipaemic plasma: An objective non-invasive photometric method to classify plasma turbidity and its association with red cell haemolysis.","authors":"Lara A E de Laleijne-Liefting, Ido J Bontekoe, Johan W Lagerberg, Thomas R L Klei","doi":"10.1111/vox.13778","DOIUrl":"10.1111/vox.13778","url":null,"abstract":"Background and objectives: Plasma components are visually inspected, and non-transparent, turbid units are rejected for transfusion and fractionation. Additionally, in case a plasma component is deemed lipaemic, there is conflicting data on the accompanying red cell concentrate (RCC) in vitro quality. As visual inspection of plasma turbidity is a subjective method, we aimed to devise an objective measurement using a quick, non-invasive, table-top spectrophotometry-based method. Using this method, the correlation between spectrophotometric data and its predictive value on haemolysis of the accompanying RCC during storage was assessed.Materials and methods: A total of 365 plasma units were visually inspected for turbidity and analysed for light reflection parameters (L*, a* and b*) and triglyceride (TG) levels. Leukoreduced RCCs in saline-adenine-glucose-mannitol (SAGM), prepared from the accompanying lipaemic whole blood, were stored for up to 6 weeks and analysed for quality parameters.Results: The light reflection L* value was the most discriminating between clear and turbid/lipaemic plasma. Also, a correlation was found between TG levels and L* values (R2 = 0.703). Plasma with TG ≥ 2.5 mmol/L showed an L* value >50 with >90% specificity and sensitivity. RCC from donations with a plasma L* value ≥68 showed significantly higher haemolysis levels (p < 0.05) during storage.Conclusion: The non-invasive photometric analysis of plasma turbidity correlated both with visual inspection and plasma TG levels. Measurement of L* values of plasma may be helpful in identifying donations with high TG levels and higher risk for increased haemolysis during RCC storage.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"277-283"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restless legs syndrome among blood donors: A systematic review and meta-analysis. 献血者中的不宁腿综合征：一项系统回顾和荟萃分析。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2024-12-10 DOI: 10.1111/vox.13780

Alain M Ngoma, Paulin B Mutombo, Mahmoud Mosli, Magot D Omokoko, Kenneth E Nollet, Hitoshi Ohto

{"title":"Restless legs syndrome among blood donors: A systematic review and meta-analysis.","authors":"Alain M Ngoma, Paulin B Mutombo, Mahmoud Mosli, Magot D Omokoko, Kenneth E Nollet, Hitoshi Ohto","doi":"10.1111/vox.13780","DOIUrl":"10.1111/vox.13780","url":null,"abstract":"Background and objectives: Restless legs syndrome (RLS), with adverse health outcomes, has been linked to blood donation, but evidence published thus far has not been rigorously analysed. This systematic review aggregates existing evidence on RLS among blood donors and identifies associated factors worthy of further investigation.Materials and methods: MEDLINE and EMBASE were searched for articles published through 16 December 2023. Eleven studies from eight countries were selected from 142 publications. The pooled prevalence of RLS was calculated using a random-effects model, with heterogeneity assessed by the Cochran Q and I2 statistics. Meta-regression and sensitivity analyses explored sources of heterogeneity and the robustness of findings.Results: Eleven studies, involving 20,255 blood donors, were included. The pooled prevalence of RLS among blood donors was 10.30% (95% confidence interval [CI]: 5.54%-16.30%), which was significantly higher than in the general adult population (3.0%, 95% CI: 1.4%-3.8%). Meta-regression identified the year of study and geographical region as significant sources of heterogeneity. From the five studies that used logistic regression analyses, female sex and older age stand out as associated factors. No publication bias was detected, and sensitivity analysis confirmed the robustness of results.Conclusion: Our findings suggest a high burden of RLS among blood donors, underscoring the need for further research with standardized criteria, appropriate design and analytical methodologies to better understand the impact of RLS on individual donors and the global blood supply.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"246-255"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oropouche virus in Brazil: Assessing the risks and challenges for transfusion medicine. 巴西的Oropouche病毒：评估输血医学的风险和挑战。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2024-12-19 DOI: 10.1111/vox.13784

Lucca Rocha Policastro, Debora Glenda Lima de La-Roque, Rodrigo Tocantins Calado, Dimas Tadeu Covas, Luiz Carlos Alcantara, Marta Giovanetti, Simone Kashima

引用次数: 0

The relationship between blood test results and vasovagal reactions: An intra-individual comparative retrospective analysis of blood donor data in Japan. 血液检查结果与血管迷走神经反应之间的关系：日本献血者数据的个体内比较回顾性分析。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2025-01-02 DOI: 10.1111/vox.13787

Taeko Chijiiwa, Akie Hirata, Tasuku Okui, Junko Iwasaki, Naoki Nakashima, Midori Kumagawa

{"title":"The relationship between blood test results and vasovagal reactions: An intra-individual comparative retrospective analysis of blood donor data in Japan.","authors":"Taeko Chijiiwa, Akie Hirata, Tasuku Okui, Junko Iwasaki, Naoki Nakashima, Midori Kumagawa","doi":"10.1111/vox.13787","DOIUrl":"10.1111/vox.13787","url":null,"abstract":"Background and objectives: To determine whether intra-individual differences in pre-donation blood test results were associated with vasovagal reactions (VVRs).Materials and methods: The study included donors who voluntarily donated 400 mL of whole blood at least twice during a 5-year blood collection period of the Japanese Red Cross, including both donations with and without a VVR. A conditional logistic regression analysis by age group and sex was conducted, using each donor as a stratum, together with an analysis of deviance to test the significance of including an interaction term between age group and blood data in the regression model.Results: A total of 1873 donors were included in the analysis. Haemoglobin, haematocrit, total protein and albumin values prior to donation were higher when a VVR was observed than when it was not for each age group and sex. The regression analysis showed significant positive associations between each of these blood parameters and VVR in all combinations of age groups and sex. A particularly strong positive association between haemoglobin and VVRs is seen in women aged ≥50 years (odds ratio, OR [95% confidence interval, CI]: 1.844 [1.457, 2.333]).Conclusion: Haemoglobin, haematocrit, total protein and albumin levels within individual donors were significantly higher at donation with a VVR. This was most notable in women aged ≥50 years. Comparing the pre-donation haemoglobin value with past values in the same donor at the donation site would help raise awareness of the risk of VVRs.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"260-267"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and analysis of residual leucocytes from leucoreduced red blood cell units. 白细胞还原红细胞单位中残留白细胞的分离与分析。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2024-11-25 DOI: 10.1111/vox.13775

Rena Hirani, Melinda M Dean, David O Irving

{"title":"Isolation and analysis of residual leucocytes from leucoreduced red blood cell units.","authors":"Rena Hirani, Melinda M Dean, David O Irving","doi":"10.1111/vox.13775","DOIUrl":"10.1111/vox.13775","url":null,"abstract":"Background and objectives: Leucoreduction is used to remove donor leucocytes during red blood cell (RBC) manufacture. However, not all are removed, and long-term survival of donor leucocytes, termed transfusion-associated microchimerism (TAM), has been shown to occur in some patients following RBC transfusion. The mechanism of TAM occurrence is unknown. One hypothesis is that viable donor haematopoietic cells remain within RBC units that could engraft. However, the analysis of cells remaining within leucoreduced RBC units has been minimal. This study aimed to isolate and analyse any residual leucocytes recovered from leucoreduced RBC units.Materials and methods: Leucoreduced RBC units were analysed on Day 1 (n = 4) and Day 42 (n = 4) post collection. Residual leucocytes were isolated using the EasySep™ RBC Depletion Reagent. Cell type analysis was conducted by flow cytometry using a leucocount reagent, a viability marker (7-amino-actinomycin D [7AAD]) and specific antibodies to CD45 and CD34. A representative 'pre-filter' sample was also obtained at the time of whole-blood donation to ensure expected cell counts across the donor samples.Results: Analysis of the pre-filter sample showed that CD45+/CD34+ cells accounted for 0.02%-0.07% of all leucocytes. Up to 253,850 residual leucocytes were isolated across both storage timepoints, and of these, up to 48 cells were CD45+/CD34+/7AAD-.Conclusion: Viable CD45+/CD34+ cells were isolated from leucoreduced RBC units, indicating the potential for donor progenitor cells to be present during transfusion. Further characterization of these residual cells is required to explain how TAM may occur in some patients following RBC transfusion.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"310-314"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

International Forum on Genotyping of Blood Antigens in Donors: Summary. 献血者血液抗原基因分型国际论坛：综述。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1111/vox.13790

Jennie Haw, Kelly Holloway, Gwen Clarke, Galit Eliyahu Nuni, Liora Muncher, James Daly, Jacqui Martin, Klaus Rieneck, Morten H Dziegiel, Cinzia Paccapelo, Luisa Ronzoni, Diarmaid O'Donghaile, Helen Ryan, Celina Montemayor Garcia, Cheuk-Kwong Lee, Wai-Chiu Tsoi, Hironori Kobayashi, Hiroyuki Igarashi, Tuulia Palukka, Katri Haimila, Nuria Nogues, Cecilia Gonzalez-Santesteban, Aaron J Gottschalk, Åsa Hellberg, Martin L Olsson, Thierry Peyrard, Ute Jentsch, Ai Leen Ang, Chew Yen Phang, Lorna Wall, Dhana Gounder, Nancy Dunbar

引用次数: 0

Longitudinal assessment of erythrogram parameters in response to granulocytapheresis frequency: A sex-based analysis. 红细胞图参数对粒细胞穿刺频率响应的纵向评估：基于性别的分析。

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-03-01 Epub Date: 2025-01-01 DOI: 10.1111/vox.13788

Abdullah Alswied, Leonard N Chen, Kamille Aisha West-Mitchell

{"title":"Longitudinal assessment of erythrogram parameters in response to granulocytapheresis frequency: A sex-based analysis.","authors":"Abdullah Alswied, Leonard N Chen, Kamille Aisha West-Mitchell","doi":"10.1111/vox.13788","DOIUrl":"10.1111/vox.13788","url":null,"abstract":"Background and objectives: Granulocyte transfusion supports patients with severe neutropenia. Maintaining a pool of eligible donors and optimizing donation frequency are essential for ensuring an adequate supply while safeguarding donor well-being. This study investigates the impact of donation frequency on erythrogram parameters, focusing on sex-specific differences.Study design and methods: We conducted a retrospective analysis of 343 successive granulocyte collections from 65 apheresis donors over 11 years (2012-2023). Donors were categorized by sex, and erythrogram parameters were analysed in relation to donation frequency and intervals.Results: Frequent donations within a short inter-donation interval (≥3 in 14 days) affected subsequent pre-donation haemoglobin levels. Each additional donation within 14 days led to a decrease of 0.81 g/dL in haemoglobin (p = 0.017). A significant interaction between sex and donations within 14 days (β = 0.76, p = 0.018) indicated that frequent donations had a more pronounced negative effect on haemoglobin levels in female donors. The proportion of donations meeting the pre-donation haemoglobin eligibility criteria declined with each successive donation within 14 days (100% at first, 85.8% at second, 25% at third). Female donors showed a significant haemoglobin reduction over three donations within 14 days (13.4-11.6 g/dL, p = 0.005) compared to males (14.4 -14 g/dL, p = 0.95).Conclusion: Short inter-donation intervals have a more pronounced negative effect on pre-donation haemoglobin levels in female donors, underscoring the need for individualized donation guidelines to ensure donor safety.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":"268-276"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influenza and COVID-19 vaccination in Canadian blood donors: A comparison across pre- and post-pandemic periods.

IF 1.8 4区医学

Vox Sanguinis Pub Date : 2025-02-27 DOI: 10.1111/vox.70006

Sheila F O'Brien, Lori Osmond, Mindy Goldman, Steven J Drews

{"title":"Influenza and COVID-19 vaccination in Canadian blood donors: A comparison across pre- and post-pandemic periods.","authors":"Sheila F O'Brien, Lori Osmond, Mindy Goldman, Steven J Drews","doi":"10.1111/vox.70006","DOIUrl":"https://doi.org/10.1111/vox.70006","url":null,"abstract":"Background and objectives: Seasonal vaccinations reduce donor illness and appointment cancellations and ensure plasma products have antibodies to vaccine-directed strains. We aimed to describe donor influenza and COVID-19 vaccination history and compare this with the general population.Materials and methods: Two online donor surveys were carried out in 2021 and 2024. Donors were asked about demographics, influenza (2019/2020, 2020/2021 and 2023/2024 seasons) and COVID-19 (ever and 2023/2024 season) vaccination and reasons for vaccination choices. General population vaccination statistics were extracted from public reports. Percentages of donors receiving vaccination were calculated with 95% confidence intervals. Multiple logistic regression models were fitted with demographics as independent variables.Results: In survey 1, 4582 (30.4% response rate) donors completed a questionnaire; in survey 2, 6376 (21% response rate). More donors under age 65 received the influenza vaccine compared with the general population under age 65 (58% vs. 30% in 2019/2020, 63% vs. 28% in 2023/2024, p < 0.0001) and aged 65+ (81% vs. 70% in 2019/2020, 90% vs. 73% in 2023/2024, p < 0.0001). Fewer donors and the general population received the COVID-19 vaccine in 2023/2024 (under 65 45% vs. 39%; 65+ 76% vs. 67%, p < 0.0001). Most said they were vaccinated to prevent infection and protect others.Conclusion: Seasonal vaccination rates are higher in older donors, consistent with public health recommendations. Blood donors are more likely to be vaccinated against seasonal influenza than the general population, but post-pandemic uptake of the COVID-19 booster vaccine was low, more similar to the general population.","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0