Dean Pym, Oleg Grinberg, Amanda J Davies, Jessica O Williams, Christine Saunders, Chloë E George, Philip E James
{"title":"储存用于新生儿输血的血小板浓缩物中氧可用性的增强与躁动无关：来自直接血氧测定和菲克扩散模型的证据。","authors":"Dean Pym, Oleg Grinberg, Amanda J Davies, Jessica O Williams, Christine Saunders, Chloë E George, Philip E James","doi":"10.1111/vox.70101","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>Platelet transfusions are essential for mitigating the bleeding risk of neonatal patients with thrombocytopenia. As neonatal patients have a small blood volume, adult therapeutic dose platelet units are split into reduced-volume storage containers to maximize the use of the donated product and reduce donor exposure. The shelf-life of platelets stored in reduced-volume containers, however, is limited to 5 days. Agitation in platelet concentrate (PC) storage is thought to promote gaseous exchange by maintaining a gradient of O<sub>2</sub> across the bag film; however, recent studies have shown that agitation-induced shear promotes the progressive decline of platelet quality over storage.</p><p><strong>Materials and methods: </strong>Electron paramagnetic resonance oximetry and Fickian diffusion modelling of O<sub>2</sub> were used to investigate the differences in O<sub>2</sub> availability, by assessing the O<sub>2</sub> concentration, oxygen consumption rate (OCR), influx of O<sub>2</sub>, total PC OCR and O<sub>2</sub> distribution in PCs stored under routine conditions in neonatal (Macopharma, VQE605B) versus adult (Haemonetics, ATSBC1ESE) PC storage containers. The influence of agitation on neonatal PC storage was evaluated.</p><p><strong>Results: </strong>Results indicate neonatal PCs experience significantly higher O<sub>2</sub> availability compared to adult PCs and can withstand greater insult to their ambient O<sub>2</sub> concentration. Adjusting the agitation frequency of neonatal PCs stored from 20 to 400 rpm had no detrimental effect on O<sub>2</sub> availability, compared to storage at 60 rpm.</p><p><strong>Conclusion: </strong>Neonatal PCs can maintain higher O<sub>2</sub> availability and tolerate reduced agitation without compromising oxygenation; therefore, reduced agitation strategies may be a feasible option to minimize shear during storage without compromising O<sub>2</sub> availability.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced O<sub>2</sub> availability in platelet concentrates stored for neonatal transfusion is independent of agitation: Evidence from direct oximetry and Fickian diffusion modelling.\",\"authors\":\"Dean Pym, Oleg Grinberg, Amanda J Davies, Jessica O Williams, Christine Saunders, Chloë E George, Philip E James\",\"doi\":\"10.1111/vox.70101\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>Platelet transfusions are essential for mitigating the bleeding risk of neonatal patients with thrombocytopenia. As neonatal patients have a small blood volume, adult therapeutic dose platelet units are split into reduced-volume storage containers to maximize the use of the donated product and reduce donor exposure. The shelf-life of platelets stored in reduced-volume containers, however, is limited to 5 days. Agitation in platelet concentrate (PC) storage is thought to promote gaseous exchange by maintaining a gradient of O<sub>2</sub> across the bag film; however, recent studies have shown that agitation-induced shear promotes the progressive decline of platelet quality over storage.</p><p><strong>Materials and methods: </strong>Electron paramagnetic resonance oximetry and Fickian diffusion modelling of O<sub>2</sub> were used to investigate the differences in O<sub>2</sub> availability, by assessing the O<sub>2</sub> concentration, oxygen consumption rate (OCR), influx of O<sub>2</sub>, total PC OCR and O<sub>2</sub> distribution in PCs stored under routine conditions in neonatal (Macopharma, VQE605B) versus adult (Haemonetics, ATSBC1ESE) PC storage containers. The influence of agitation on neonatal PC storage was evaluated.</p><p><strong>Results: </strong>Results indicate neonatal PCs experience significantly higher O<sub>2</sub> availability compared to adult PCs and can withstand greater insult to their ambient O<sub>2</sub> concentration. Adjusting the agitation frequency of neonatal PCs stored from 20 to 400 rpm had no detrimental effect on O<sub>2</sub> availability, compared to storage at 60 rpm.</p><p><strong>Conclusion: </strong>Neonatal PCs can maintain higher O<sub>2</sub> availability and tolerate reduced agitation without compromising oxygenation; therefore, reduced agitation strategies may be a feasible option to minimize shear during storage without compromising O<sub>2</sub> availability.</p>\",\"PeriodicalId\":23631,\"journal\":{\"name\":\"Vox Sanguinis\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vox Sanguinis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/vox.70101\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vox Sanguinis","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/vox.70101","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
Enhanced O2 availability in platelet concentrates stored for neonatal transfusion is independent of agitation: Evidence from direct oximetry and Fickian diffusion modelling.
Background and objectives: Platelet transfusions are essential for mitigating the bleeding risk of neonatal patients with thrombocytopenia. As neonatal patients have a small blood volume, adult therapeutic dose platelet units are split into reduced-volume storage containers to maximize the use of the donated product and reduce donor exposure. The shelf-life of platelets stored in reduced-volume containers, however, is limited to 5 days. Agitation in platelet concentrate (PC) storage is thought to promote gaseous exchange by maintaining a gradient of O2 across the bag film; however, recent studies have shown that agitation-induced shear promotes the progressive decline of platelet quality over storage.
Materials and methods: Electron paramagnetic resonance oximetry and Fickian diffusion modelling of O2 were used to investigate the differences in O2 availability, by assessing the O2 concentration, oxygen consumption rate (OCR), influx of O2, total PC OCR and O2 distribution in PCs stored under routine conditions in neonatal (Macopharma, VQE605B) versus adult (Haemonetics, ATSBC1ESE) PC storage containers. The influence of agitation on neonatal PC storage was evaluated.
Results: Results indicate neonatal PCs experience significantly higher O2 availability compared to adult PCs and can withstand greater insult to their ambient O2 concentration. Adjusting the agitation frequency of neonatal PCs stored from 20 to 400 rpm had no detrimental effect on O2 availability, compared to storage at 60 rpm.
Conclusion: Neonatal PCs can maintain higher O2 availability and tolerate reduced agitation without compromising oxygenation; therefore, reduced agitation strategies may be a feasible option to minimize shear during storage without compromising O2 availability.
期刊介绍：
Vox Sanguinis reports on important, novel developments in transfusion medicine. Original papers, reviews and international fora are published on all aspects of blood transfusion and tissue transplantation, comprising five main sections:
1) Transfusion - Transmitted Disease and its Prevention:
Identification and epidemiology of infectious agents transmissible by blood;
Bacterial contamination of blood components;
Donor recruitment and selection methods;
Pathogen inactivation.
2) Blood Component Collection and Production:
Blood collection methods and devices (including apheresis);
Plasma fractionation techniques and plasma derivatives;
Preparation of labile blood components;
Inventory management;
Hematopoietic progenitor cell collection and storage;
Collection and storage of tissues;
Quality management and good manufacturing practice;
Automation and information technology.
3) Transfusion Medicine and New Therapies:
Transfusion thresholds and audits;
Haemovigilance;
Clinical trials regarding appropriate haemotherapy;
Non-infectious adverse affects of transfusion;
Therapeutic apheresis;
Support of transplant patients;
Gene therapy and immunotherapy.
4) Immunohaematology and Immunogenetics:
Autoimmunity in haematology;
Alloimmunity of blood;
Pre-transfusion testing;
Immunodiagnostics;
Immunobiology;
Complement in immunohaematology;
Blood typing reagents;
Genetic markers of blood cells and serum proteins: polymorphisms and function;
Genetic markers and disease;
Parentage testing and forensic immunohaematology.
5) Cellular Therapy:
Cell-based therapies;
Stem cell sources;
Stem cell processing and storage;
Stem cell products;
Stem cell plasticity;
Regenerative medicine with cells;
Cellular immunotherapy;
Molecular therapy;
Gene therapy.
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