Vox Sanguinis最新文献

{"title":"Determination of CD177 (human neutrophil antigen 2) polymorphisms using nanopore sequencing.","authors":"Kirstine Kløve-Mogensen, Thure Mors Haunstrup, Anne-Louise Fjordside Bilde, Rudi Steffensen","doi":"10.1111/vox.70020","DOIUrl":"https://doi.org/10.1111/vox.70020","url":null,"abstract":"<p><strong>Background and objectives: </strong>Human neutrophil antigen 2 (HNA-2), encoded by the CD177 gene, is considered one of the most important neutrophil antigens in human medicine, but molecular testing of CD177 is complicated and therefore not a standard procedure for investigating CD177 expression. CD177 expression can vary from 0% to 100%, and to date, the molecular basis for altered or non-expressed genes has not been determined. Reliance on phenotyping and crossmatching to investigate these neutropenic clinical cases is inconvenient for patients and demands substantial resources within the laboratory. The purpose of this study was therefore to test a new molecular testing approach based on long-read nanopore sequencing.</p><p><strong>Materials and methods: </strong>DNA from 44 Danish blood donors with different levels of CD177 expression, 22 of whom were found to be CD177 null, was selected as test samples. All the DNA was sequenced for the first eight exons and the beginning of exon 9 of CD177.</p><p><strong>Results: </strong>All incidences of CD177 null cases could be associated with the known variant c.787A>T;p.K263X (rs20182172), and a correlation was observed between c.787A>T heterozygosity and a reduced expression of CD177, which is consistent with previously published findings. The c.1291G>A;p.G431R (rs78718189) variant was found to be linked to the atypical expression of CD177. The nanopore assay revealed a total of 14 variants in 7 exons in the 44 tested samples.</p><p><strong>Conclusion: </strong>On the basis of these observations, we conclude that long-read nanopore sequencing can be a reliable tool for the routine laboratory molecular testing of CD177.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Forum on the Use of Irradiated Blood in Patients With Haematological Malignancy: Summary.","authors":"Xiao-Yin Zhang, Michael Murphy, Graham P Collins, Vernon J Louw, Gamuchirai Y Tadzimirwa, Satyam Arora, Nita Radhakrishnan, Carlos Gonzalez, Ryan A Metcalf, Erica Swenson, Maria A Nuñez, Edgardo Saa, Arwa Z Al-Riyami, Ali Tabatabaey, Yulia Lin, Aditya Tedjaseputra, Erica M Wood, Alexandra Pedraza, Cristina Sanz, Corentin Streel, Véronique Deneys, Amalia G Bravo, Karla Maldonado, Luiz Amorim, Thais Ferraz, Yoshihiko Tani, Naoko Goto, Fanny Delettre, Pierre Tiberghien, Jaap Jan Zwaginga, Theodora Foukaneli, Paul Kerr, Samclide Mbikayi Mutindu, Alphonse Mosolo Nganzele, Richard Schäfer, Nancy Dunbar","doi":"10.1111/vox.70015","DOIUrl":"https://doi.org/10.1111/vox.70015","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction in cord blood graft potency due to processing delay can be prevented by trehalose supplementation.","authors":"Suria Jahan, Harinad B Maganti, Jaina Patel, Roya Pasha, Emily Doxtator, Javed K Manesia, Nicolas Pineault","doi":"10.1111/vox.70012","DOIUrl":"https://doi.org/10.1111/vox.70012","url":null,"abstract":"<p><strong>Background and objectives: </strong>Maximizing the potency of cord blood (CB) units (CBU) is a goal of all CB banks. Low transplant cell dose in CB transplantation is one of several contributors to the slower engraftment. Processing delays could also be at play because CBU can be stored at room temperature (RT) for up to 48 h before cryopreservation. We hypothesized that prolonged storage at RT reduces the potency and engraftment activities of CBU due to the loss of haematopoietic stem cells (HSCs) and progenitor cells.</p><p><strong>Materials and methods: </strong>CBU were split in half, with one half processed shortly after collection and the other half after 40 h of storage at RT. Viabilities and potencies were compared post thaw, and engraftment activity was tested in xenotransplants. Bioinformatic analysis was carried out to identify pathways modulated by prolonged storage at RT.</p><p><strong>Results: </strong>Prolonged storage at RT reduced the viability of CB cells and potency of grafts based on in vitro and in vivo assays. Transcriptomic analyses revealed that paracrine factors released in CBU induce several changes in gene expression programmes in CD34+ cells. These included the activation of the senescence gene CDKN1B and down-regulation of cell cycle and autophagy genes. Supplementation of CBU with trehalose prevented the loss in cell viability and potency. The latter was associated with the restoration of autophagy and suppression of the senescence gene CDKN1B.</p><p><strong>Conclusion: </strong>These results stress the importance of rapid processing of CBU and support further investigations on trehalose as a natural supplement to protect CBU' potency during storage.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation of red cell concentrates in non-DEHP plasticized containers compared with standard DEHP plasticized containers: A quasi-randomized observational multicentre study.","authors":"Christie Vermeulen, Nan van Geloven, Bart Mertens, Saskia E Spelmink, Martin R Schipperus, Mart P Janssen, Jean-Louis H Kerkhoffs, Gijs den Besten, Annegeet G van den Bos, Irene M L W Körver-Keularts, Daan van de Kerkhof, Harriët J H Klinkspoor, Karen M K De Vooght, Adriaan J van Gammeren, Henk Russcher, Josephine W M Heijnen, Dirk de Korte, Pieter F van der Meer, Thomas R L Klei","doi":"10.1111/vox.70019","DOIUrl":"https://doi.org/10.1111/vox.70019","url":null,"abstract":"<p><strong>Background and objectives: </strong>The use of di-ethyl-hexyl-phthalate (DEHP) in medical devices will be banned in the European Union from 1 July 2030, onwards. It is therefore important to evaluate the performance of non-DEHP blood collection system alternatives. Previously, we reported that red cell concentrates (RCCs) in phosphate-adenine-glucose-guanosine-saline-mannitol (PAGGSM) stored in non-DEHP blood containers did not result in an increased transfusion reaction rate (TRR) as compared with saline-adenine-glucose-mannitol (SAGM)/DEHP-stored RCC. The current study aimed to extend the dataset and confirm previous findings.</p><p><strong>Materials and methods: </strong>A quasi-randomized observational study was conducted to compare the number and type of transfusion reactions. A 95% credibility interval for the TRR odds ratio (OR) was calculated.</p><p><strong>Results: </strong>A total of 7507 BTHC/PAGGSM and 25,371 DEHP/SAGM RCCs were transfused, yielding a TRR of 0.35% (0.22%-0.49%) and 0.20% (0.15%-0.26%), respectively, with an OR of 1.76 (1.10-2.81). Pooling data of the current and previous study resulted in an OR of 1.36 (0.90-2.08). Restricting the pooled data to imputability categories 'possible', 'probable' and 'definite' yielded an OR of 1.09 (0.66-1.81).</p><p><strong>Conclusion: </strong>An increased TRR was observed for BTHC/PAGGSM in the new dataset, though it was still consistent with those of DEHP when aggregating data from both studies.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Removal of deferrals for variant Creutzfeldt-Jakob disease risk: Impact on new and previously deferred donors.","authors":"Mindy Goldman, David McKee, Shane Smith, Sheila F O'Brien","doi":"10.1111/vox.70021","DOIUrl":"https://doi.org/10.1111/vox.70021","url":null,"abstract":"<p><strong>Background and objectives: </strong>Concern over variant Creutzfeldt-Jakob disease (vCJD) led to the deferral of donors who had resided in the United Kingdom since January 1980. This deferral was implemented in 1999 and subsequently modified to include other countries. Some deferrals were removed in February 2022; deferrals for the United Kingdom, Ireland and France were removed on 22 November 2023. In this study, we describe efforts made to encourage donation from newly eligible people and the resulting donation gain.</p><p><strong>Materials and methods: </strong>Actions targeted individual donors deferred after 1 January 2012. Marketing included website, social media and general advertising. Staff asked first-time donors if the criteria change had motivated their donation. Deferred and returning donor data were determined from our donor database.</p><p><strong>Results: </strong>In the 12 months post-implementation, 12.8% of first-time donors surveyed were newly eligible (n = 8667) and 7.8% of vCJD risk deferred donors returned (n = 5159). Eighty-five percent of deferrals occurred pre-2017; the return rate was 6.5% in this group. The highest return rate (24%) occurred in donors deferred after 2020.</p><p><strong>Conclusion: </strong>Removal of the vCJD deferrals had a major positive impact. The greatest gain was in new donors who had previously self-deferred. Despite intensive efforts, only one-quarter of recently deferred donors returned.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing iron depletion in regular platelet apheresis donors: A comparison of reticulocyte haemoglobin and serum ferritin.","authors":"Parintorn Nakdee, Thanatphak Warindpong, Sutasinee Virat, Parichart Permpikul, Janejira Kittivorapart","doi":"10.1111/vox.70023","DOIUrl":"https://doi.org/10.1111/vox.70023","url":null,"abstract":"<p><strong>Background and objectives: </strong>Frequent platelet apheresis donations can lead to iron depletion. Serum ferritin is traditionally measured to assess iron status in donors. Reticulocyte haemoglobin (RET-He) has emerged as a rapid, convenient and cost-effective alternative. This study aimed to determine the prevalence of iron depletion among regular platelet apheresis donors and to evaluate the sensitivity and specificity of RET-He compared to serum ferritin in diagnosing iron depletion.</p><p><strong>Materials and methods: </strong>This cross-sectional study involved 100 healthy, regular platelet apheresis donors aged 18-60 years who donated at least once a month. RET-He, serum ferritin and complete blood count results were analysed. The sensitivity and specificity of RET-He were calculated against serum ferritin.</p><p><strong>Results: </strong>Of the 100 donors, 13.00% (13 out of 100 donors) had serum ferritin levels <15 ng/mL, indicating iron depletion. RET-He showed a sensitivity of 76.92% (95% confidence interval [95% CI]: 46.19%-94.96%) and specificity of 44.83% (95% CI: 34.15%-55.87%) in detecting iron depletion. Significant differences in RET-He levels, donation frequency, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, white blood cell count and red cell distribution width were detected between the iron-depleted and non-iron-depleted groups (p = 0.045, 0.032, 0.053, 0.026, 0.069 and 0.027, respectively).</p><p><strong>Conclusion: </strong>Frequent platelet donations increase the risk of iron depletion; therefore, iron supplementation is recommended for regular donors. While RET-He testing is convenient, cost effective and fast, it cannot replace serum ferritin testing because of insufficient sensitivity and specificity.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facilitators of and barriers to blood donation among voluntary non-remunerated blood donors in sub-Saharan Africa: A scoping review.","authors":"Austrida Gondwe, Effie Chipeta, Mina C Hosseinipour, Bridon Mbaya, Adamson S Muula, Victor Mwapasa, Patani Mhango, Princess Kaira, Tiyamike Nthani, Sydney Puerto-Meredith, Natasha Nsamala, Emmanuel Singogo","doi":"10.1111/vox.70013","DOIUrl":"https://doi.org/10.1111/vox.70013","url":null,"abstract":"<p><strong>Background and objectives: </strong>In many countries, including Africa, the widespread gap between national blood requirements and actual blood supplies contributes to unnecessary deaths. This scoping review explores common facilitators and barriers to blood donation in sub-Saharan Africa (SSA) and strategies that are used to increase and maintain repeat blood donation.</p><p><strong>Materials and methods: </strong>We conducted a scoping review of facilitators and barriers to blood donation in SSA. We searched PubMed, Ovid MEDLINE, Ovid EMBASE and COCHRANE to identify relevant papers. Of the 2225 articles screened by title, abstract and papers published after 2010, 37 were included in the final full-text screening. Article quality for inclusion was assessed on the basis of a predefined eligibility and inclusion checklist. We analysed all papers that reported barriers and facilitators to blood donation in Africa.</p><p><strong>Results: </strong>The review included 11 studies. The common facilitators for blood donation reported were altruism, the opportunity for disease testing, friendly recruitment strategies and future easy access to blood at hospitals when needed. We identified the following barriers: lack of knowledge, awareness of blood donation and place of donation, fear, ineffective incentives, bad service experiences and religious and cultural beliefs.</p><p><strong>Conclusion: </strong>Findings of this review can be used to propose better strategies for improving blood donation in SSA. Strategies that encourage blood donation can be leveraged and implemented, while those that discourage blood donation can be adapted to better achieve an adequate supply.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma exchange in Susac syndrome: Unlocking treatment for a rare disease.","authors":"Yamac Akgun","doi":"10.1111/vox.70017","DOIUrl":"https://doi.org/10.1111/vox.70017","url":null,"abstract":"","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet transfusion stated practices among neonatal and paediatric veno-arterial extracorporeal membrane oxygenation providers: A survey.","authors":"Trupti Ingle, Brian Simms, Jesse Bain, Melania M Bembea, Jill M Cholette, Madhuradhar Chegondi, Eva Cheung, Robert Niebler, Caroline Ozment, Matthew Paden, Ofer Schiller, Marianne E Nellis, Oliver Karam","doi":"10.1111/vox.70018","DOIUrl":"https://doi.org/10.1111/vox.70018","url":null,"abstract":"<p><strong>Background and objectives: </strong>Extracorporeal membrane oxygenation (ECMO) provides cardiopulmonary support to over 4000 neonates and children annually worldwide. Although potentially lifesaving, ECMO carries bleeding and thromboembolic risks, often managed with platelet transfusions to maintain specific thresholds. Platelet transfusions themselves carry many risks. This survey aimed to describe stated prophylactic platelet transfusion practices among paediatric veno-arterial (VA)-ECMO providers and identify factors influencing transfusion decisions.</p><p><strong>Materials and methods: </strong>This is a cross-sectional electronic survey of paediatric ECMO providers from 10 centres evaluating platelet transfusion thresholds based on six patient scenarios (non-bleeding, minimally bleeding and resolved bleeding in neonates and children). Descriptive statistics were used for analysis.</p><p><strong>Results: </strong>The survey response rate was 56% (114 of 204). Paediatric intensivists comprised 66% of respondents. The median pre-transfusion platelet count varied across VA-ECMO scenarios, with a threshold of 50 × 10⁹/L (interquartile range [IQR] 45-75) for non-bleeding children and 70 × 10⁹/L (IQR 50-85) for non-bleeding neonates. The threshold for minimally bleeding children, minimally bleeding neonates and resolved bleeding in children was 75 × 10⁹/L (IQR 50-100). The threshold for resolved bleeding in neonates was 80 × 10⁹/L (IQR 50-100). There was significant heterogeneity between and within sites (p < 0.001). Uncertainty about the level of evidence was high (59%), with clinical judgement being the most influential factor in transfusion decisions (85%).</p><p><strong>Conclusion: </strong>Prophylactic platelet transfusion practices in paediatric ECMO vary widely, highlighting uncertainty and the need for clinical trials to improve patient outcomes.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of an A_weakB phenotype caused by significant ABO gene deletion in a Chinese woman.","authors":"Tian Gao, Feiyu Jiang, Shoufang Xu, Yingying Zhang, Weiwei Zheng, Xinhui Wang, Zhiwei Liu","doi":"10.1111/vox.70008","DOIUrl":"https://doi.org/10.1111/vox.70008","url":null,"abstract":"<p><strong>Background and objectives: </strong>The A_weakB is a rare phenotype in the ABO blood group system. Here, we first report a novel ABO mutation discovered in a Chinese woman with an A_weakB. Third-generation sequencing was employed to investigate the molecular mechanisms underlying A_weakB. By correctly identifying the phenotype, it was useful for increasing the safety of blood transfusion.</p><p><strong>Materials and methods: </strong>ABO blood group was identified by the standard ABO serological test and polymerase chain reaction with sequence-specific primers (PCR-SSP). To analyse the ABO gene sequence, single-molecule real-time (SMRT) sequencing was performed to obtain full-length sequencing of the ABO gene.</p><p><strong>Results: </strong>The Chinese individual was serologically identified as A_weakB subtype, and SMRT sequencing analysis revealed that there were large fragment deletion mutations in the promoter (c.1-1326_1-1321del, c.1-1010_1-975del, c.1-952_1-1del) and Exon 1 region (c.1_28del).</p><p><strong>Conclusion: </strong>We report for the first time that large fragment deletions represent a novel molecular basis for the A_weakB. These deletions may potentially influence the expression of the A antigen.</p>","PeriodicalId":23631,"journal":{"name":"Vox Sanguinis","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}