Shwatina Jagnarine, Daniel Guobadia, Tayler A Van Denakker, Emmalene Kyritsis, Tiffany A Thomas, Krystalyn E Hudson
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引用次数: 0
Abstract
Background and objectives: During cold storage, red blood cells (RBCs) undergo progressive biochemical and structural changes, known as the storage lesion, which affects their function and post-transfusion recovery (PTR). While anticoagulants and additive solutions help mitigate these effects, they also influence unit haematocrit. The impact of haematocrit on PTR remains unclear. This study investigates how storage of haematocrit, storage duration and recipient sex influence PTR.
Materials and methods: Using transgenic mice with RBCs expressing fluorescent proteins, leukoreduced RBC units were prepared and stored at 60% or 75% haematocrit for 1, 6 or 11 days. Before transfusion, freshly collected RBCs were added to stored units to facilitate PTR calculations. RBC units were then transfused into female or male recipient mice and PTR was evaluated at multiple time points.
Results: PTR decreased with storage duration. Haematocrit had no significant effect on PTR for RBC units stored up to 6 days. However, after 11 days of storage, a higher haematocrit was associated with reduced PTR. Additionally, female recipients exhibited significantly lower PTR than male recipients, regardless of unit haematocrit.
Conclusion: Haematocrit and recipient sex influence PTR for RBC units stored for a long time. These findings highlight the need to consider both factors when optimizing RBC storage and transfusion strategies.
期刊介绍：
Vox Sanguinis reports on important, novel developments in transfusion medicine. Original papers, reviews and international fora are published on all aspects of blood transfusion and tissue transplantation, comprising five main sections:
1) Transfusion - Transmitted Disease and its Prevention:
Identification and epidemiology of infectious agents transmissible by blood;
Bacterial contamination of blood components;
Donor recruitment and selection methods;
Pathogen inactivation.
2) Blood Component Collection and Production:
Blood collection methods and devices (including apheresis);
Plasma fractionation techniques and plasma derivatives;
Preparation of labile blood components;
Inventory management;
Hematopoietic progenitor cell collection and storage;
Collection and storage of tissues;
Quality management and good manufacturing practice;
Automation and information technology.
3) Transfusion Medicine and New Therapies:
Transfusion thresholds and audits;
Haemovigilance;
Clinical trials regarding appropriate haemotherapy;
Non-infectious adverse affects of transfusion;
Therapeutic apheresis;
Support of transplant patients;
Gene therapy and immunotherapy.
4) Immunohaematology and Immunogenetics:
Autoimmunity in haematology;
Alloimmunity of blood;
Pre-transfusion testing;
Immunodiagnostics;
Immunobiology;
Complement in immunohaematology;
Blood typing reagents;
Genetic markers of blood cells and serum proteins: polymorphisms and function;
Genetic markers and disease;
Parentage testing and forensic immunohaematology.
5) Cellular Therapy:
Cell-based therapies;
Stem cell sources;
Stem cell processing and storage;
Stem cell products;
Stem cell plasticity;
Regenerative medicine with cells;
Cellular immunotherapy;
Molecular therapy;
Gene therapy.
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