Virology Journal最新文献

{"title":"Clinical symptoms and molecular epidemiologic characteristics of varicella patients among children and adults in Ganzhou, China.","authors":"Ting Zeng, Chao-Xian Lian, Xiao-Yi Zhang, Ping-Qing Liu, Jian Ao, Gang-Feng Zhou, Xiao-Dong Chen, Dan-Dan Huang, Dian-Gui Hu, Xin Chen","doi":"10.1186/s12985-025-02661-6","DOIUrl":"10.1186/s12985-025-02661-6","url":null,"abstract":"<p><strong>Background: </strong>Varicella-zoster virus (VZV) is highly transmissible; however, there are limited studies in China.</p><p><strong>Methods: </strong>The clinical symptoms, disease progression, and laboratory test results of varicella patients among children and adults diagnosed at Ganzhou Fifth People's Hospital from August 2021 to December 2022 were analysed retrospectively. Genetic polymorphisms in the open reading frame (ORF) 22 and ORF62 fragments of VZV isolates were analysed using molecular epidemiological methods.</p><p><strong>Results: </strong>Thirty-nine varicella patients were included in this study, 26 of them were children and 13 of them were adults. The incidence of discomfort and complications was significantly greater in adults than in children (P < 0.05). Four adults developed severe disease, one of whom died, with no cases of severe disease or death among children. The 32 VZV clinical isolates were all Clade 2 wild-type strains. Four variant isolates from children had eight base mutations, five of which were missense; two variant isolates from adults had four base mutations, all of which were missense.</p><p><strong>Conclusions: </strong>The risk of developing severe disease or even death after VZV infection in adults was greater than that in children. There is an urgent need for more studies focusing on the differences in the pathogenicity of VZV in different age groups.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"44"},"PeriodicalIF":4.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11844084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the link between parvovirus B19 and encephalitis: a systematic review and comprehensive meta-analysis of molecular and serological evidence.","authors":"Kashmi Sharma, Rekha Khandia, Rohan Shrivastava, Ram K Nema, Somesh Mishra, Rupinder K Kanwar, Ashwin A Raut, Amit Agrawal, Vandana Gupta, Megha K Pandey","doi":"10.1186/s12985-025-02630-z","DOIUrl":"10.1186/s12985-025-02630-z","url":null,"abstract":"<p><p>Encephalitis, a severe brain inflammation, can arise due to various infectious agents, including viruses like Parvovirus B19 (B19V). Previously linked to mild neonatal and young one's illnesses and some haematological diseases, recent evidence associates B19V with encephalitis, with no clear prevalence and mechanisms in place. This systematic review and meta-analysis aim to determine the prevalence of B19V in cases of encephalitis, exploring variations associated with diagnostic approaches, and identifying gaps in existing research to enhance clinical comprehension and diagnostic methods. An extensive search (1994-2024) was performed through PubMed, Scopus, ScienceDirect, and Cochrane databases for research and epidemiological investigations related to B19V in cases of encephalitis. Inclusion criteria focused on studies that verified B19V using molecular (PCR, NGS) or serological (IgM/IgG) techniques in cerebrospinal fluid or serum. Data analysis was done to pool the prevalence data of included studies using a random-effects model. Heterogeneity was evaluated using I² statistics. Sensitivity and meta-regression analyses were conducted to evaluate variability and the effects of moderators. A total of fourteen studies involving 3,135 encephalitis patients resulted in a combined prevalence of 3% (95% CI: 2-4%). Studies using PCR indicated a greater prevalence (3%) in comparison to ELISA (1%) and NGS (2%). A moderate level of heterogeneity (I² = 57.4%) was attributed to the variability in diagnostic methods and geographic distribution. Sensitivity analyses validated strong estimates, while meta-regression revealed country as a key moderator accounting for heterogeneity. Publication bias was modest. The research indicates that B19V may be involved in certain encephalitis instances, with an overall prevalence of 3%. The differences observed in the studies emphasize the need for standardized diagnostic procedures and more extensive multicentric epidemiological research.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"42"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11837615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of SARS-CoV-2 in nasopharyngeal swab samples from COVID-19 patients in Riyadh, Saudi Arabia: a PCR-based study.","authors":"Rania Ali El Hadi Mohamed, Shikha Fahad Alojayan, Albandary Nasser Alsaloom, Sheka Yagub Aloyouni, Khawlah Aldilaijan, Sarah Abdullah Ababtain","doi":"10.1186/s12985-025-02655-4","DOIUrl":"10.1186/s12985-025-02655-4","url":null,"abstract":"<p><p>This study involved laboratory experiments using conventional PCR to detect the RNA-dependent RNA polymerase protein (RdRp) and Envelope (E) genes in Forty-Seven nasopharyngeal swab samples from COVID-19 patients in Riyadh, Saudi Arabia. Gel electrophoresis results showed amplification of the RdRp gene in 85.1% of the samples and the E gene in 89.4%, confirming the widespread presence of these viral genes. The presence of bands in positive controls indicated the specificity of the primers whilst no bands were detected in the negative controls, indicating the absence of contamination. The study also included data collection from databases to explore the demographic and clinical characteristics of COVID-19 patients. The male to female infection ratio was 363:63, significantly favoring males (P ≤ 0.05). Fever was present in 81.46% of patients (P ≤ 0.05). A significant portion (60.56%) had not contacted positive cases or traveled outside Saudi Arabia (P ≤ 0.05). The Saudi to non-Saudi ratio among patients was 24.65-75.35% (P ≤ 0.05). Age distribution showed 62.21% of patients were under 50 years old (P ≤ 0.05). ICU admission was required for 12.21% of patients (P ≤ 0.05). Co-morbidities were present in 27.46% of patients (P ≤ 0.05). The mortality rate was low, with a deceased to alive ratio of 1:141 (P ≤ 0.05). Gel electrophoresis revealed that 85.1% of samples showed amplification for the RdRp gene, and 89.4% for the E gene, confirming the widespread presence of these viral genes among the samples tested.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"43"},"PeriodicalIF":4.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11837285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic analysis of human papillomavirus in cervical exfoliated cells from women in Zigong.","authors":"Xiaoyang Ma, Chuan Wu, Tao Wu, Xiaolin Yu, Lixing Song","doi":"10.1186/s12985-025-02652-7","DOIUrl":"10.1186/s12985-025-02652-7","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the human papillomavirus (HPV) infection status among women in Zigong from January 2016 to August 2024 and provides a comprehensive statistical analysis of HPV infection characteristics. The findings aim to enhance cervical cancer screening, inform vaccination strategies, and improve HPV infection prevention measures.</p><p><strong>Methods: </strong>We conducted a retrospective analysis on 48,474 female patients who visited the gynecology department of Zigong Fourth People's Hospital from January 2016 to August 2024. Cervical exfoliated cell samples were collected from the patients, and the genotypes of 10 low-risk HPV (LR-HPV) and 17 high-risk HPV (HR-HPV) were detected by flow fluorescent hybridization technique. The study explored HPV infection rates, genotype distribution, number of infections, type of infections, and age distribution. The chi-squared (χ²) test was employed to compare infection statuses between groups.</p><p><strong>Results: </strong>Among the 48,474 patients, 9749 tested positive for HPV, with an overall infection rate of 20.11%. The HPV infection rate increased gradually from 2016 to 2024 (P < 0.001). The infection rates of single, double, triple, and ≥ quadruple infections were 15.11%, 3.54%, 1.00%, and 0.46%, respectively. The infection rates were 4.41% for LR-HPV-only, 13.13% for HR-HPV-only, and 2.57% for mixed LR and HR-HPV. HR-HPV primarily consisted of HPV types 52, 16, 53, and 58, with infection rates of 3.94%, 2.71%, 2.43%, and 2.42%, respectively. LR-HPV primarily consisted of types 61 and 81, with infection rates of 1.64% and 1.49%, respectively. A significant age correlation in HPV infection was observed (P < 0.001), with two distinct peaks in infection rates.</p><p><strong>Conclusions: </strong>The HPV infection rate among women visiting the gynecology department in Zigong is high, predominantly involving HPV types 52, 16, 53, and 58. Therefore, strengthening HPV screening efforts and focusing on standardized genotype screening is crucial. Additionally, selecting HPV vaccines targeting prevalent genotypes and actively conducting HPV prevention and control work can reduce the incidence of HPV-related cervical cancer and other HPV-related diseases.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"40"},"PeriodicalIF":4.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11831773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term inhibition of Hepatitis B virus gene expression by a primary microrna expressing ancestral adeno-associated viral vector.","authors":"Njabulo Ziphezinhle Mnyandu, Shonisani Wendy Limani, Abdullah Ely, Reubina Wadee, Patrick Arbuthnot, Mohube Betty Maepa","doi":"10.1186/s12985-025-02662-5","DOIUrl":"10.1186/s12985-025-02662-5","url":null,"abstract":"<p><p>Current treatments for chronic infection with the hepatitis B virus (HBV) rarely cure carriers from the disease. Previously reported use of serotype 8 adeno-associated viral (AAV8) vectors to deliver expression cassettes encoding anti-HBV artificial primary microRNAs (apri-miRs) has shown promise in preclinical studies. A recently designed synthetic ancestral AAV (Anc80L65) with high liver transduction efficiency is a promising new addition to the anti-HBV vector toolbox. This study engineered Anc80L65 to express HBx-targeting apri-miRs. Single dose administration of the vectors to cultured cells and HBV transgenic mice effected reductions of secreted HBV surface antigen (HBsAg). Circulating HBV particles and HBV core antigen (HBcAg) were also significantly diminished in mice receiving the anti-HBV apri-miR-expressing ancestral AAVs. Downregulation of HBV biomarkers occurred over a period of 12 months. Absence of inflammatory responses or liver toxicity indicated that the vectors had a good safety profile. These data suggest that a single dose of apri-miR-expressing Anc80L65 is safe and capable of mediating durable suppression of HBV gene expression. Targeting HBx, which is required for transcriptional activity of covalently closed circular DNA of HBV, makes this Anc80L65-derived vector a promising candidate for functional cure from chronic HBV infection.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"41"},"PeriodicalIF":4.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A surrogate in vitro experimental model for off-label drug repurposing: inhibitory effect of montelukast on bovine respiratory syncytial virus replication.","authors":"Hanne Nur Kurucay, Zafer Yazici, Vahide Bayrakal, Bahadir Muftuoglu, Emre Ozan, Cuneyt Tamer, Seda Gozel, Gerald Barry, Mahir Igde, Semra Okur-Gumusova, Harun Albayrak, Ahmed Eisa Elhag, Huseyin Baskin","doi":"10.1186/s12985-025-02647-4","DOIUrl":"10.1186/s12985-025-02647-4","url":null,"abstract":"<p><strong>Background: </strong>Repurposing off-label drugs during epidemics or pandemics with unknown/known pathogens, particularly when their side effects and complications are already known, can be a strategic approach, as seen during the COVID-19 pandemic. Developing surrogate in vitro experimental models (passage-to-passage), which mimic epidemic/pandemic-like transmission (human-to-human), may enhance this repurposing process. This study evaluates montelukast sodium (MLS), a US FDA-approved leukotriene receptor antagonist for asthma, to explore its potential repurposing antiviral effects against bovine respiratory syncytial virus (BRSV), which has basic similarities to human respiratory syncytial virus (HRSV) as both belong to the Pneumoviridae family.</p><p><strong>Methods: </strong>An in vitro serial passage model was developed using MDBK cells infected with a local wild-type strain of BRSV (43TR2018). The cytotoxicity of MLS was assessed via the trypan blue exclusion method, identifying non-toxic concentrations. The impact of MLS on viral spread and infectivity was measured through TCID50 values over 10 passages. Viral loads were confirmed by nested RT-PCR and quantified using qPCR, while apoptosis, necrosis, and nitric oxide production were evaluated through staining and nitrite assays. Data were analyzed using ANOVA and Tukey's test (p < 0.05).</p><p><strong>Results: </strong>Control cells exhibited 97.16% viability, with 10 µM and 20 µM MLS concentrations maintaining viabilities of 89.2% and 87.3%, respectively. Viral titers significantly decreased at higher concentrations of MLS (up to 99.94% inhibition). Apoptosis rates decreased in MLS-treated cells, and live cell percentages improved, especially at 20 µM. Nitric oxide levels showed no significant differences across groups.</p><p><strong>Conclusion: </strong>MLS demonstrated a dose-dependent antiviral effect against BRSV, achieving 99% viral inhibition properties in MDBK cells. These promising results warrant further investigation into the antiviral mechanisms of MLS.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"38"},"PeriodicalIF":4.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of thrombotic thrombocytopenic purpura induced by acute hepatitis E and successfully controlled by lymphoplasmapheresis plus rituximab.","authors":"Binfeng Yu, Yongfen Zhu, Zhihua Jin, Fei Han, Fangfang Lv","doi":"10.1186/s12985-025-02649-2","DOIUrl":"10.1186/s12985-025-02649-2","url":null,"abstract":"<p><strong>Background: </strong>Thrombocytopenia is a common extrahepatic manifestation of hepatitis E virus (HEV) infection and is usually transient and self-limited. Thrombotic thrombocytopenic purpura (TTP) is a rare and lethal blood disorder characterized by microangiopathic hemolytic anemia, severe thrombocytopenia, and organ involvement. The link between HEV infection and TTP is still unclear.</p><p><strong>Case presentation: </strong>A 74-year-old female was referred to our hospital with complaints of fever, fatigue, nausea and jaundice for 10 days. Liver dysfunction, positive IgM and IgG of HEV, and HEV-RNA viremia prompted the diagnosis of acute hepatitis E, which was followed by a dramatic decline in the platelet count. The presence of schistocytes in the peripheral blood smear, along with decreased ADAMTS13 activity, strongly suggested a diagnosis of TTP. Combination therapy, including 2 courses of lymphoplasmapheresis (LPE), 4 courses of therapeutic plasma exchange, glucocorticoids and rituximab, was applied and contributed to the recovery of platelet. No recurrence of TTP was observed during the follow-up period. To date, this is first patient who developed the initial episode of TTP during the course of HEV viremia. In the meanwhile, LPE was used for the first time in the treatment of HEV-associated TTP.</p><p><strong>Conclusions: </strong>This case highlights the necessity of ruling out TTP in hepatitis E patients with newly developed and severe thrombocytopenia and the values of LPE plus rituximab in treating such patients.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"39"},"PeriodicalIF":4.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse effects of coronavirus-defective viral genomes on the synthesis of IFNβ and ISG15 mRNAs and coronavirus replication.","authors":"Hsuan-Wei Hsu, Li-Kang Chang, Chun-Chun Yang, Ching-Hung Lin, Yu Teng, Pei-Chi Hsu, Cheng-Yao Yang, Hung-Yi Wu","doi":"10.1186/s12985-025-02654-5","DOIUrl":"10.1186/s12985-025-02654-5","url":null,"abstract":"<p><strong>Background: </strong>The mechanism by which coronavirus-defective viral genomes (DVGs) affect coronavirus and host cells during infection remains unclear. A variety of DVGs with different RNA structures can be synthesized from coronavirus-infected cells, and these DVGs can also encode proteins. Consequently, in the present study, we first dissected the effects of individual DVGs on the synthesis of IFNβ and ISG15 mRNAs at the RNA, protein and combined levels, and then examined whether different coronavirus-DVGs have different effects on the synthesis of IFNβ and ISG15 mRNAs and coronavirus replication both individually and collectively under different infection conditions.</p><p><strong>Methods: </strong>To dissect the effects of individual DVGs on the synthesis of IFNβ and ISG15 mRNAs at the RNA, protein and combined levels, DVG 2.2 and DVG 5.1, which were previously identified in coronavirus-infected cells, and their mutants were constructed followed by transfection. Western blot and RT‒qPCR were used to detect the synthesis of protein and to quantify the synthesis of IFNβ and ISG15 mRNAs, respectively. To examined whether different coronavirus-DVGs have different effects on the synthesis of IFNβ and ISG15 mRNAs and coronavirus replication both individually and collectively under different infection conditions, different naturally occurring DVGs were selected and constructed followed by transfection after or before coronavirus infection and by RT‒qPCR and hemagglutination assay.</p><p><strong>Results: </strong>These results suggested that (i) coronavirus-DVGs at the RNA, protein and combined levels have different effects on the synthesis of IFNβ and ISG15 mRNAs, (ii) coronavirus-DVGs can inhibit coronavirus replication at least partly through interferon signaling and (iii) different DVGs have different effects on the synthesis of IFNβ and ISG15 mRNAs and coronavirus replication both individually and collectively under different infection conditions.</p><p><strong>Conclusions: </strong>Coronavirus replication can be regulated by diverse coronavirus-derived DVGs at least partly through innate immunity. Such regulation may contribute to the pathogenesis of coronavirus. The DVG populations in coronavirus-infected cells with the ability to inhibit coronavirus replication are expected to be potential resources for the identification of antivirals at the level of RNA, protein or in combination, and the methods used in the current study can be used as a platform for this purpose.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"37"},"PeriodicalIF":4.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected HBsAg decrease after nucleoside analogues retreatment among HBeAg positive postpartum women: a pilot study.","authors":"Qiao Tang, Chunrui Wang, Hu Li, Zhiwei Chen, Li Zhang, Jing Zhang, Xiaoqing Liu, Yunling Xue, Yue Qiu, Mingli Peng, Yi Zeng, Peng Hu","doi":"10.1186/s12985-025-02632-x","DOIUrl":"10.1186/s12985-025-02632-x","url":null,"abstract":"<p><strong>Background: </strong>Mother-to-child transmission (MTCT) is one of the main routes of transmission of HBV, and previous studies focused on the efficacy and safety of nucleoside analogues (NAs) in preventing MTCT. There are limited data on virologic changes of chronic hepatitis B (CHB) patients after discontinuing treatment postpartum and the efficacy of retreatment.</p><p><strong>Methods: </strong>A retrospective-prospective real-world pilot cohort study on pregnant women with CHB was conducted. Biochemical and virological characteristics (HBsAg, HBeAg and HBV DNA) in patients received NAs treatment pre-pregnancy (n = 24), patients discontinued treatment after delivery (n = 88) and retreatment patients (n = 22) were collected during follow-up.</p><p><strong>Results: </strong>The incidences of HBeAg clearance, half decrease of HBsAg, 0.5 lg decrease of HBsAg and HBsAg < 1000 IU/mL in patients discontinuing treatment postpartum were 5.7% (4/70), 10.0% (8/48), 6.3% (3/48) and 1.6% (1/61), respectively. More significantly decreases of HBsAg, HBeAg and HBV DNA were observed in retreatment patients compared to patients received NAs treatment pre-pregnancy. Significantly higher cumulative incidences of half decrease of HBsAg, 0.5 lg decrease of HBsAg and HBsAg < 1000 IU/mL were achieved in retreatment patients compared to patients received NAs treatment pre-pregnancy. Long-term follow-up results indicated that it is safe for HBeAg positive pregnant patients to discontinue treatment after delivery.</p><p><strong>Conclusion: </strong>HBeAg positive patients received NAs treatment during pregnancy and discontinued it postpartum can benefit from NAs retreatment because of unexpected decrease of HBsAg, which may be helpful for achieve the goal of functional cure. Trial registration number ClinicalTrials.gov (No.ChiCTR2100054116).</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"36"},"PeriodicalIF":4.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of hepatitis B virus infection and associated factors among adults intrafamilial household contacts attending antenatal care clinics in the Central Ethiopian region: from pregnant women index cases.","authors":"Yilma Markos Larebo, Abebe Alemu Anshebo, Sujit Kumar Behera, Natarajan Gopalan","doi":"10.1186/s12985-025-02633-w","DOIUrl":"10.1186/s12985-025-02633-w","url":null,"abstract":"<p><strong>Background: </strong>In Ethiopia, hepatitis B virus infections are prevalent and highly endemic. Additionally, there has been a significant increase in hospital admissions, morbidity, and mortality associated with hepatitis B virus infections. This study aimed to assess the prevalence of hepatitis B virus infection and associated factors among adult intrafamilial household contacts of pregnant women index cases attending antenatal care clinics in the central Ethiopian region.</p><p><strong>Methods: </strong>A community-based cross-sectional study was conducted between October 1, 2023, and March 1, 2024. Three hundred eighty-five adult intrafamilial household contacts were randomly selected via lottery methods. A 3 ml venous blood sample was taken from adult intrafamilial household contacts and checked for hepatitis B virus infection through hepatitis B surface antigen. An interviewer-administered questionnaire was used to collect the data. A logistic regression model predicted the relationship between predictor and outcome variables. A p-value of < 0.05 indicated statistical significance.</p><p><strong>Results: </strong>The overall response rate was 96.1%. Two-thirds of the adults of intrafamilial household contacts (n = 229; 61.9%) were aged between 18 and 28 years, with a mean age of 28 years. The prevalence rate of hepatitis B virus infection among adults of intrafamilial household contacts with pregnant women as the index case was 11.6% (95% CI, 8.6 to 15.1). Being male (AOR: 0.09; 95% CI: 0.03, 0.37) and a duration of stay with the index case of less than six months (AOR: 0.30; 95% CI: 0.11, 0.81) were associated with a reduced risk of hepatitis B virus infection. Meanwhile, large family sizes (≥ 7) (AOR: 4.32; 95% CI: 1.34, 13.98), genital discharge (AOR: 3.14; 95% CI: 1.60, 6.15), engagement in unsafe sex (AOR: 2.37; 95% CI: 1.13, 4.97), and a history of mortality due to hepatitis in the family (AOR: 3.03; 95% CI: 1.09, 8.42) were associated with an increased risk of hepatitis B virus infection.</p><p><strong>Conclusion: </strong>This study found that hepatitis B surface antigen seropositivity among adult intrafamilial household contacts with pregnant women index cases in the central Ethiopia region was high at 11.6%. These findings suggest that interventions to prevent HBV infection should prioritize educational campaigns targeting adult intrafamilial household contacts of HBV-positive index cases, focusing on risk factors associated with HBV transmission, prevention, counselling, testing, and vaccination.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"34"},"PeriodicalIF":4.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11823231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}