Virology Journal最新文献

{"title":"Increase of VEGF and Fibronectin expression and ultrastructural alterations of intercellular junctions in a swab negative patient after SARS-COV-2 infection.","authors":"Carolina Simioni, Juana Maria Sanz, Roberta Gafà, Giovanna Cenacchi, Savino Occhionorelli, Angelina Passaro, Luca Maria Neri","doi":"10.1186/s12985-025-02701-1","DOIUrl":"10.1186/s12985-025-02701-1","url":null,"abstract":"<p><strong>Background: </strong>SARS-CoV-2 infection has been responsible of COrona VIrus Disease (COVID-19) pandemia and can cause a variety of symptoms including gastrointestinal disorders, abdominal pain and liver injury. The host receptor for SARS-CoV-2, ACE2, is expressed in gut and SARS-CoV-2 infection could induce vascular damage and immune system dysregulation, creating an inflammatory and hypercoagulable state, as widely described at the lung level.</p><p><strong>Case presentation: </strong>This work presents the case of a middle-aged Caucasian man admitted to the Hospital Emergency Department from the University Hospital of Ferrara (Italy), complaining of pain in the upper and middle region of the abdomen. The patient tested negative to the nose-oropharyngeal swab for SARS-CoV-2 four weeks after recovering from viral infection. The patient required resection of a segment of ileum and an ulcer of the bowel wall was recognized and sampled. Previous published results had confirmed the presence of the SARS-CoV-2 nucleocapsid protein, an increased human leukocyte antigen (HLA-G) and an altered morphology of microvilli in the ulcerated ileum of the patient when compared to the non-ulcerated ileum. The present study sought to deepen the consequences of SARS-CoV-2 infection. To this end, we evaluated the expression and co-expression of Vascular Endothelial Growth Factor (VEGF) and Fibronectin by immunohistochemical techniques. VEGF immunohistochemical expression was higher in the ulcer than in the control ileum sample and the non-ulcerated ileum areas and co-expressed with the SPIKE protein. Fibronectin staining was lower in control sample than in non-ulcerated and ulcerated ileum. Electron microscopy analysis showed alterations of the integrity of the intestinal barrier in the ulcerated area when compared to the non-ulcerated ileum or to the control sample.</p><p><strong>Conclusions: </strong>Although the patient was tested negative to nose-oropharyngeal swab for SARS-CoV-2, the SPIKE protein was detected in his terminal ileum, especially in the ulcerated areas. The presence of the viral protein was also associated with an increase of VEGF and Fibronectin. In addition to vascular changes, the SARS-CoV-2 infection altered the junctional apparatus among epithelial cells, making the tissue even more fragile and thus susceptible to the entry of pathogens and the development of further infections.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"82"},"PeriodicalIF":4.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and validation of autophagy-related genes in sepsis based on bioinformatics studies.","authors":"Dong-Po Wei, Wei-Wei Jiang, Chang-Xing Chen, Zi-Yang Chen, Fang-Qing Zhou, Yu Zhang, Jian Lu","doi":"10.1186/s12985-025-02683-0","DOIUrl":"https://doi.org/10.1186/s12985-025-02683-0","url":null,"abstract":"<p><p>We identified 14 key genes associated with mitochondrial autophagy in sepsis through differential analysis of the dataset and then analysed the identified genes for functional enrichment. The analysis of key genes and deeper analysis of key genes by molecular typing, Weighted Gene Correlation Network Analysis (WGCNA) and ceRNA were also carried out. We have also validated these key genes with clinical data. Finally, sepsis diagnostic models are constructed by combining key genes with machine learning methods. In addition, we discuss the importance of the immune system in sepsis and its relationship with signature genes, which opens up new directions for studying the role of the immune system in sepsis. Overall, our study adds new ideas to the diagnosis and treatment of sepsis.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"81"},"PeriodicalIF":4.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleos(t)ide analogs continuation is not associated with a lower risk of HBsAg seroreversion following PEG-IFN-induced HBsAg loss.","authors":"Na Gao, Haishi Wu, Bin Li, Huiying Yu, Lili Wu, Jing Zhang, Nan Zhang, Bingliang Lin, Qiyi Zhao, Zhiliang Gao","doi":"10.1186/s12985-025-02700-2","DOIUrl":"10.1186/s12985-025-02700-2","url":null,"abstract":"<p><strong>Background/aims: </strong>It is unclear whether nucleos(t)ide analogs (NUCs) continuation provides clinical benefits following HBsAg seroclearance with pegylated interferon (PEG-IFN)-based therapy. This study aims to investigate the role of NUCs continuation in HBsAg seroreversion.</p><p><strong>Methods: </strong>Patients who experienced serum HBsAg loss after PEG-IFN-based therapy were enrolled and followed up for 96 weeks. Propensity score matching (PSM) was performed using a 1:1 ratio to adjust for the associated factors. A multivariate logistic regression analysis was used to determine the factors associated with HBsAg seroreversion.</p><p><strong>Results: </strong>In total, 220 patients with HBsAg seroclearance were divided into NUCs (n = 54) and non-NUCs (n = 166) consolidation therapy groups. At week 96, the HBsAg seroreversion (12/54 vs. 31/166, P = 0.709) and virological relapse (2/54 vs. 10/166, P = 0.759) rates were similar in the NUCs and non-NUCs groups. After PSM, HBsAg seroreversion (12/53 vs. 13/53; P = 1.000) and virological relapse (2/53 vs. 4/53; P = 0.674) rates were not significantly different between the two groups. Serum hepatitis B surface antibody titer (odds ratio, 0.388; 95% confidence interval, 0.245-0.616; P < 0.001) was found to be associated with HBsAg seroreversion, while NUCs continuation was not related to HBsAg seroreversion.</p><p><strong>Conclusions: </strong>NUCs continuation is not associated with a lower risk of HBsAg seroreversion in patients with serum HBsAg loss following PEG-IFN-based therapy.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"80"},"PeriodicalIF":4.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of Nanopore sequencing provides access to high quality genomic data for multi-component CRESS-DNA plant viruses.","authors":"Daniel H Otron, Denis Filloux, Andy Brousse, Murielle Hoareau, Babbitha Fenelon, Cécile Hoareau, Emmanuel Fernandez, Fidèle Tiendrébéogo, Jean-Michel Lett, Justin S Pita, Philippe Roumagnac, Pierre Lefeuvre","doi":"10.1186/s12985-025-02694-x","DOIUrl":"10.1186/s12985-025-02694-x","url":null,"abstract":"<p><strong>Background: </strong>Faced with the recrudescence of viral CRESS-DNA plant diseases, the availability of efficient and cost-effective tools for routine diagnosis and genomic characterisation is vital. As these viruses possess circular single-strand DNA genomes, they have been routinely characterised using rolling circle amplification (RCA) coupled with Sanger sequencing. However, while providing the basis of our knowledge of the diverse CRESS-DNA viruses, this approach is laboratory-intensive, time-consuming and ultimately ineffective faced with co-infection or viruses with multiple genomic components, two common characteristics of these viruses. Whereas alternatives have proved effective in some applications, there is a strong need for next-generation sequencing methods suitable for small-scale projects that can routinely produce high quality sequences comparable to the gold standard Sanger sequencing.</p><p><strong>Results: </strong>Here, we present an RCA sequencing diagnostic technique using the latest Oxford Nanopore Technology flongle flow cells. Originally, using the tandem-repeat nature of RCA products, we were able to improve the quality of each viral read and assemble high-quality genomic components. The effectiveness of the method was demonstrated on two plant samples, one infected with the bipartite begomovirus African cassava mosaic virus (ACMV) and the other infected with the nanovirus faba bean necrotic stunt virus (FBNSV), a virus with eight genomic segments. This method allow us to recover all genomic components of both viruses. The assembled genomes of ACMV and FBNSV shared 100% nucleotide identity with those obtained with Sanger sequencing. Additionally, our experiments demonstrated that for similar-sized components, the number of reads was proportional to the segment frequencies measured using qPCR.</p><p><strong>Conclusion: </strong>In this study, we demonstrated an accessible and effective Nanopore-based method for high-quality genomic characterisation of CRESS-DNA viruses, comparable to Sanger sequencing. Face with of increasing challenges posed by viral CRESS-DNA plant diseases, integrating this approach into routine workflows could pave the way for more proactive responses to viral epidemics.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"78"},"PeriodicalIF":4.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virome diversity and potential sharing of wild mammals in a biodiversity hotspot, Yunnan, China.","authors":"Yongman Guo, Chao Su, Hanwei Liang, Xueqi Jiang, Ruifu Yang, Junbin Ye, Thomas R Gillespie, Zihou Gao, Lei Xu","doi":"10.1186/s12985-025-02702-0","DOIUrl":"10.1186/s12985-025-02702-0","url":null,"abstract":"<p><strong>Background: </strong>Small mammals, including rodents, shrews and moonrats are widespread and serve as natural reservoirs for many viral pathogens. However, the composition and distribution of wild animal viromes remain poorly understood. At least 10,000 virus species have the ability to infect humans, but the vast majority are circulating silently in wild mammals. Understanding the virome profiles of these wild animals is crucial for outbreak preparedness, particularly in regions with high mammalian diversity.</p><p><strong>Methods: </strong>In this study, we enriched and extracted viral RNA from fecal samples of 459 wild mammals, representing 16 species, in the Xishuangbanna Dai Autonomous Prefecture of China, a recognized biodiversity hotspot in China. We then performed next-generation sequencing and comprehensive virome analyses across these different animal species.</p><p><strong>Results: </strong>We identified 5,346 nearly complete contigs annotated to 64 viral families, with 45 viral families identified in rodents and 46 viral families in shrews and moonrats, showing significant variation in viral diversity across different host species. Among these, 28 viral families were shared across species, including 11 identified viruses that were potential zoonotic pathogens. Additionally, numerous unidentified viral contigs containing the RdRp-gene showing close evolutionary relationships with viral families known to cause infections in animals. Importantly, several viruses detected in these animals, belonging to the family Hepeviridae, Flaviviridae, Astroviridae, Picornaviridae, and Picobirnaviridae, exhibited > 70% nucleotide sequence identity to viruses known to cause diseases in other wildlife species, domestic animals or even humans.</p><p><strong>Conclusions: </strong>These findings significantly increase our knowledge of viral diversity and potential viral transmission within rodents and other sympatric small mammals in an emerging disease hotspot, shedding light on the need for continued surveillance of these small mammal populations.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"79"},"PeriodicalIF":4.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemokines simultaneously bind SARS-CoV-2 nucleocapsid protein RNA-binding and dimerization domains.","authors":"Alberto Domingo López-Muñoz, Jonathan W Yewdell","doi":"10.1186/s12985-025-02658-1","DOIUrl":"10.1186/s12985-025-02658-1","url":null,"abstract":"<p><p>Viruses express chemokine (CHK)-binding proteins to interfere with the host CHK network and thereby modulate leukocyte migration. SARS-CoV-2 Nucleocapsid (N) protein binds a subset of human CHKs with high affinity, inhibiting their chemoattractant properties. Here, we report that both N's RNA-binding and dimerization domains participate individually in CHK binding. CHKs typically possess independent sites for binding glycosaminoglycans (GAG) and their receptor proteins. We show that the interaction with the N protein occurs through the CHK GAG-binding site, pointing the way to developing compounds that block this interaction for potential anti-coronavirus therapeutics.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"77"},"PeriodicalIF":4.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burden and characteristics of Respiratory Syncytial Virus-associated respiratory tract infections in adult patients in the winter season 2023/2024 at the conservative emergency department of the university hospital in Dresden.","authors":"J Ronczka, S von Bonin, A Laubner, K Hochauf-Stange, M Rank, M Kolditz","doi":"10.1186/s12985-025-02692-z","DOIUrl":"10.1186/s12985-025-02692-z","url":null,"abstract":"<p><strong>Introduction: </strong>The burden of Respiratory Syncytial Virus (RSV) associated adult emergency department visits in comparison to other respiratory viruses like Influenza and SARS-CoV-2 remains less studied.</p><p><strong>Methods: </strong>We performed a prospective observational study to describe prevalence, severity and risk factors of RSV infection, proven by polymerase chain reaction from nasopharyngeal or pharyngeal swabs, in consecutive adult patients presenting to the emergency ward of the University Hospital Dresden with a working diagnosis of acute respiratory tract infection during the winter season between October 1st 2023 and April 15th 2024.</p><p><strong>Results: </strong>1764 adults (56.3% male) between 18 and 101 years old (median age 69 years) were included in the analysis. 477 patients (27.1%) tested positive for viral infection; 284 (16.2%) with SARS-CoV-2 (median age 79 years), 147 (8.4%) with Influenza A or B (median age 56 years) and 38 (2.2%) with RSV A or B (median age 79 years). In 8 patients (0.5%) a co-infection with two viruses was detected. In the RSV cohort any oxygen support was significantly higher (63.2%) compared to the Influenza (34.0%, p < 0.001) and SARS-CoV-2 (41.5%, p = 0.012) cohorts. In-hospital mortality was considerable especially for RSV with 7.9% compared to Influenza (2.7%, p = 0.138) and SARS-CoV-2 (5.6%, p = 0.580).</p><p><strong>Conclusion: </strong>RSV was less frequent in adults presenting to the emergency department during the 2023/24 season compared to SARS-CoV-2 and Influenza, but patients needed a higher level of respiratory support. Also, in-hospital mortality was considerable, making RSV-infections a relevant pathogen in adult patients presenting with respiratory tract infection to an emergency department.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"76"},"PeriodicalIF":4.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic analysis and transmission networks highlight the role of older adults in the transmission of HIV-1 in northern Zhejiang, China.","authors":"Wanjun Chen, Qin Fan, Wei Cheng, Jiafeng Zhang, Lin He, Jun Jiang, Xiaoqi Liu, Xiaojuan Zhu, Hui Xing, Yi Feng, Ping Zhong, Xiaohong Pan, Chengliang Chai","doi":"10.1186/s12985-025-02681-2","DOIUrl":"10.1186/s12985-025-02681-2","url":null,"abstract":"<p><strong>Background: </strong>In China, human immunodeficiency virus (HIV) poses a significant challenge to older heterosexual adults. This study explored the transmission dynamics of HIV infection among older adults in northern Zhejiang Province, China using phylogenetic analysis and transmission networks.</p><p><strong>Methods: </strong>HIV pol sequences without any antiretroviral therapy were obtained from newly diagnosed HIV-positive patients in Huzhou between 2017 and 2022. Pairwise genetic distances between sequences were calculated using HIV Trace based on the Tamura-Nei 93 method. The transmission network was constructed using Cytoscape v3.9.1. The effective reproductive number (Re) of each large cluster was estimated using the birth-death skyline model in BEAST v2.4.2. Multivariate logistic regression was performed to identify factors associated with clustering using R v4.4.3 software.</p><p><strong>Results: </strong>A total of 931 HIV pol sequences were successfully obtained, of which CRF07_BC (51.7%, 481/931) and CRF01_AE (27.5%, 256/931) were the predominant subtypes. The proportion of CRF07_BC increased from 43.5% in 2017 to 59.8% in 2022, whereas that of CRF01_AE decreased from 33.3% in 2017 to 19.5% in 2022. In total, 448 individuals formed 110 putative transmission networks with a clustering rate of 48.1%, ranging from 2 to 83 sequences per network. Four large clusters were identified, with a higher proportion of individuals aged ≥ 50 years (49.0%) compared to that in small/ medium clusters (35.5%) and non-clustered cases (26.1%). Multivariable logistic regression showed that clustering was associated with age ≥ 50 years (adjusted odds ratio [aOR] = 2.125, 95% confidence interval [CI]: 1.251-3.632), registered households in Huzhou (aOR: 1.677, 95% CI: 1.252-2.249), and CRF07_BC subtype (aOR: 2.119, 95% CI: 1.542-2.924). Only one of the four large transmission clusters had a Re > 1, with a high proportion (63.0%, 29/46) of male older adults exposed through commercial sexual contact.</p><p><strong>Conclusions: </strong>The subtype of CRF07_BC was the predominant subtype locally, showing an increasing trend over time. Molecular transmission network analysis and multivariate logistic regression revealed that older adults play a key role in local HIV-1 transmission. Public health services should target this key population to curb the spread of HIV-1.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"75"},"PeriodicalIF":4.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered LY6E and TRIM6 expression in PBMCs correlated with HBsAg clearance and response to Peg-IFN-α treatment in HBeAg-negative chronic hepatitis B patients.","authors":"Yiru Shan, Hao Pang, Yao Tang, Na Yang, Rui Wang, Fan Yang, Bo Qin","doi":"10.1186/s12985-025-02689-8","DOIUrl":"10.1186/s12985-025-02689-8","url":null,"abstract":"<p><strong>Background: </strong>Pegylated interferon alpha (Peg-IFN-α) has the potential to eradicate hepatitis B surface antigen (HBsAg). This study aimed to investigate whether the expression levels of lymphocyte antigen 6 family member E (LY6E) and tripartite motif-containing protein 6 (TRIM6) mRNAs in peripheral blood mononuclear cells (PBMCs) of hepatitis B e antigen (HBeAg)-negative chronic hepatitis B virus (HBV) patients is associated with the response to Peg-IFN-α treatment and HBsAg clearance.</p><p><strong>Methods: </strong>In this prospective study, HBeAg-negative chronic HBV patients treated with Peg-IFN-α were followed for 48 weeks. The participants were classified into two groups, the virological response (VR) group and nonvirological response (NVR) group, according to the changes in HBV DNA and HBsAg levels observed at week 48 of treatment. Furthermore, these patients were divided into a serological response (SR) group and a nonserological response (NSR) group, depending on whether they exhibited a loss of serum HBsAg or evidence of seroconversion. The expression levels of LY6E and TRIM6 mRNAs in PBMCs were evaluated using real-time quantitative PCR with fluorescence detection. The diagnostic performance of LY6E and TRIM6 was assessed by analyzing the receiver operating characteristic (ROC) curve and calculating the area under the ROC curve (AUC).</p><p><strong>Results: </strong>After the treatment period, the observed VR and SR rates were 44.64% and 28.57%, respectively. Dynamic changes in LY6E and TRIM6 mRNA levels were significantly different between the VR and NVR groups and between the SR and NSR groups. Multivariate analysis revealed that TRIM6 was independently associated with VR at weeks 12 and 24 of Peg-IFN-α therapy and with SR at week 12; in addition, LY6E was independently associated with VR at week 12 and SR at week 24. At week 24, the area under the curve (AUC) for LY6E in the prediction of VR was 0.6942, and the AUC for the prediction of SR was 0.7766; at week 12, TRIM6 had AUCs of 0.7600 for the prediction of VR and 0.8469 for the prediction of SR.</p><p><strong>Conclusions: </strong>LY6E and TRIM6 are important biomarkers for early therapeutic responses to Peg-IFN-α and HBsAg clearance.</p><p><strong>Trial registration: </strong>Registration number: 2023 - 311. Date of registration: 1 October 2023.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"74"},"PeriodicalIF":4.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of human papillomavirus genotypes infections in patients with cervical lesions and cervical cancer in Urumqi, Xinjiang from 2016 to 2023.","authors":"Yan Wang, Reyilanmu Maisaidi, Shihan Zhang, Yibanuer Reheman, Lili Han","doi":"10.1186/s12985-025-02674-1","DOIUrl":"10.1186/s12985-025-02674-1","url":null,"abstract":"<p><strong>Background: </strong>The persistence of high-risk human papillomavirus (HPV) infection is well-established as a key etiological factor in the progression to cervical intraepithelial neoplasia (CIN) and cervical cancer (CC). This study aims to investigate the clinical and epidemiological characteristics associated with HR-HPV infections diagnosed in conjunction with cervical intraepithelial lesions in Urumqi, Xinjiang.</p><p><strong>Methods: </strong>Between 2016 and 2023, we collected clinical data from 4,389 patients with cervical lesions who underwent colposcopic histopathological examination at the People's Hospital of Xinjiang Uygur Autonomous Region. Cervical samples were obtained for HPV DNA genotyping and cytological analysis. Patients presenting with cervical abnormalities or abnormal cytology results subsequently underwent cervical biopsy.</p><p><strong>Results: </strong>The prevalence of HPV infection among 4,389 patients with cervical lesions were found to be 98.95% (4,345/4,389). Specifically, the prevalence of HPV types 16 and 18 were 78.87% (1,314/1,666). The five most common genotypes identified were HPV types 16, 52, 58, 31, and 33, with infection rates of 34.57%, 19.54%, 12.45%, 8.98%, and 7.66%, respectively. Among the patients with cervical lesions, cervical inflammation was observed in 522 individuals (11.90%), while the distribution of cervical intraepithelial neoplasia (CIN) was as follows: CIN I in 644 patients (14.67%), CIN II in 1,067 patients (24.31%), CIN III in 1,041 patients (23.72%), and CC in 1,115 patients (25.40%). The distribution of patients in the CC group was most prevalent among those aged ≥ 60 years (47.99%, 322/671). A high prevalence was also observed in the 30~39 year age group within the CIN III group (29.47%, 275/933). Han and Uygur patients accounted for 85.90% of cervical lesion cases (3,770/4,389). Hui patients were predominantly identified within the CIN II group (34.12%), whereas Uighur patients were most frequently observed in CC group (36.60%) (P < 0.005).</p><p><strong>Conclusions: </strong>Patients with cervical lesions had high HPV prevalence in Urumqi, Xinjiang. The five most prevalent HPV types identified in this population are HPV 16, 52, 58, 31, and 33. Epidemiological studies focusing on high-risk HPV types hold significant clinical implications, particularly in informing and guiding HPV vaccination strategies.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"72"},"PeriodicalIF":4.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}