Virology Journal最新文献

{"title":"Correction: Characterization of the epidemiology, susceptibility genes and clinical features of viral infections among children with inborn immune errors: a retrospective study.","authors":"Haiqiao Zhang, Wenjie Wang, Qinhua Zhou, Jia Hou, Wenjing Ying, Xiaoying Hui, Jinqiao Sun, Lipin Liu, Luyao Liu, Chenhao Wang, Hai Zhang, Bijun Sun, Xiaochuan Wang","doi":"10.1186/s12985-025-02808-5","DOIUrl":"https://doi.org/10.1186/s12985-025-02808-5","url":null,"abstract":"","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"200"},"PeriodicalIF":4.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the genetic diversity of the 3' untranslated region (UTR) of Dengue virus.","authors":"Alessandra Lo Presti, Arnold Knijn, Enrico Palermo, Alessandra D'Auria, Miriana Quaranta, Stefano Pascarella, Federica Frasca, Carolina Scagnolari","doi":"10.1186/s12985-025-02800-z","DOIUrl":"10.1186/s12985-025-02800-z","url":null,"abstract":"<p><strong>Background: </strong>Dengue virus (DENV), with its four antigenically distinct serotypes, is the etiological agent of dengue fever, which is endemic in tropical and subtropical regions but has recently spread to previously non-endemic areas such as Europe. Given the growing body of evidence suggesting that sequence diversification in the 3' UTR of DENV contributes to its epidemiological fitness and host adaptation, we conducted phylogenetic and genetic diversity analyses on four DENV 3' UTR datasets (DENV-1, DENV-2, DENV-3 and DENV-4).</p><p><strong>Methods: </strong>A maximum likelihood phylogenetic analysis was performed using IQ-TREE, and the average evolutionary divergence was estimated using MEGA X. Maximum likelihood analysis combined with genetic distance calculations provided insight into the evolutionary dynamics of the DENV 3' UTR.</p><p><strong>Results: </strong>A higher number of supported internal clusters/clades were found in the DENV-2 and DENV-1 3' UTR trees, probably indicating strains with similar evolutionary histories. In terms of cluster composition, apart from a general mixing of DENV 3' UTR sequences from different sites and years, the majority of supported internal clusters were composed of sequences aggregated according to their location. Genetic distances showed that the DENV-1 3' UTR has a higher variability (5%) compared to DENV-2 (3%), DENV-3 and DENV-4 (2%). The average length of the 3' UTR, as estimated from our datasets, showed that it was longest in DENV-2, followed by DENV-3, DENV-1 and DENV-4.</p><p><strong>Conclusions: </strong>In conclusion, this study provided a comprehensive analysis of 3' UTR evolution and phylogenies in all four DENV serotypes, suggesting that this viral genomic sequence is subject to genetic variability and length changes.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"197"},"PeriodicalIF":4.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells.","authors":"Hengshan Zhu, Chuang Lou, Ping Liu","doi":"10.1186/s12985-025-02824-5","DOIUrl":"10.1186/s12985-025-02824-5","url":null,"abstract":"","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"196"},"PeriodicalIF":4.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human mastadenovirus pneumonia in two immunocompetent patients.","authors":"Salome Hosch, Baptiste Hamelin, Jasmin D Haslbauer, Christopher M Field, Albert Heim, Lucas Boeck, Spasenija Savic Prince, Tanja A Gradistanac, Anita E Gander, Peter M Keller, Karoline Leuzinger, Kirsten D Mertz","doi":"10.1186/s12985-025-02823-6","DOIUrl":"10.1186/s12985-025-02823-6","url":null,"abstract":"<p><strong>Background: </strong>Human adenoviruses (hAdV) are common pathogens associated with acute respiratory tract infections. Mastadenovirus blackbeardi (hAdV-B) has been linked to severe pneumonia and disseminated disease, primarily in immunocompromised patients. The histopathological and clinical features of adenoviral pneumonia remain poorly characterized.</p><p><strong>Case presentation: </strong>We report two cases of hAdV-B pneumonia in immunocompetent female patients with a history of smoking. Both patients are of Caucasian origin and reside in Switzerland. They presented with persistent respiratory symptoms, pulmonary infiltrates on CT scans, and necrotizing granulomatous inflammation in lung tissue. After excluding other pathogens, metagenomic whole-genome sequencing identified hAdV-B in both cases. Both patients recovered fully following wedge resection of the affected lung tissue, without the need for additional therapy.</p><p><strong>Conclusions: </strong>These two cases demonstrated a remarkably similar clinical and histopathological profile, characterized by chronic granulomatous lung inflammation. The findings suggest a prolonged inflammatory response leading to persistent tissue damage. Our observations highlight the potential of hAdV-B to cause chronic pneumonia even in otherwise healthy individuals.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"198"},"PeriodicalIF":4.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in HPV-positive cervical cancer prevalence: a retrospective study from 2013 to 2020.","authors":"Xiaohui Yang, Shiyuan Qi, Liyan Dai, Qingjian Ye, Xiaomao Li","doi":"10.1186/s12985-025-02830-7","DOIUrl":"10.1186/s12985-025-02830-7","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer ranks as the fourth most common cancer among women globally, mainly linked to persistent high-risk HPV infection. In China, significant challenges persist, notably the low rates of HPV vaccination and a substantial case burden. This study retrospectively examined HPV-related cervical cancer data from a tertiary hospital spanning the years 2013 to 2020, to evaluate annual and age-specific incidence trends and offer insights for prevention and treatment strategies.</p><p><strong>Methods: </strong>The retrospective analysis encompassed patients who utilized the gynecological outpatient and inpatient services at the Third Affiliated Hospital of Sun Yat-sen University from 2013 to 2020. HPV genotyping, covering 21 subtypes, was conducted using hybrid capture-based assays. The assessment of cervical biopsy disease etiology was performed by a senior pathologist.</p><p><strong>Results: </strong>During the study period, a total of 9,194 cases of HPV-positive individuals were identified, among which 479 cases (5.21%) were diagnosed with cervical cancer. From 2013 to 2020, there was a statistically significant decline in the overall incidence of HPV-positive cervical cancer (Z=-4.061, P < 0.001), as well as in the incidence of squamous cell carcinoma (Z=-4.595, P < 0.001). However, the incidence of adenocarcinoma did not exhibit a significant change (Z = 0.118, P = 0.906). Regarding age distribution, a significant decrease in cervical cancer incidence was observed in patients aged 36 to 64 years (Z=-2.658, P = 0.008). In contrast, the incidence remained relatively stable in patients aged 65 years and older (Z = 0.071, P = 0.943). The incidence of squamous cell carcinoma increased with age, peaking at 27.06% in individuals over 65 years.</p><p><strong>Conclusion: </strong>The decline in HPV-positive cervical cancer cases highlights the success of China's screening and post-2016 vaccination efforts. However, rising HPV infections in younger women and ongoing cancer risks in older groups emphasized the need to broaden adolescent vaccinations, sexual health education, and lifelong screening.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"199"},"PeriodicalIF":4.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monkeypox virus and type 1 diabetes: a molecular insight into inflammatory signaling and β-cell autoimmunity.","authors":"Yahya A Almutawif, Haydar M Al-Kuraishy, Ali I Al-Gareeb, Ali K Albuhadily, Hamza M A Eid, Athanasios Alexiou, Marios Papadakis, Mohammed E Abo-El Fetoh, Gaber El-Saber Batiha","doi":"10.1186/s12985-025-02822-7","DOIUrl":"10.1186/s12985-025-02822-7","url":null,"abstract":"<p><p>The current worldwide pandemic of monkeypox (MPXV) elicited apprehensions over its possible long-term health ramifications. Recent data indicate a potential association between MPXV infection and the onset of autoimmune disorders, such as type 1 diabetes (T1D). This article analyzes the possible methods for MPXV infection to induce autoimmune responses and damage pancreatic β-cells. We examine from published articles the possible MPXV-induced immune dysregulation and inflammation in the development of T1D. Utilizing insights from other diabetogenic viruses, such as enterovirus and SARS-CoV-2. Currently, the association between MPXV and the development of T1D is scarce. Therefore, additional studies are essential for establishing a conclusive causal link between MPXV and T1D.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"195"},"PeriodicalIF":4.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of cytomegalovirus in glioblastoma development: promoter or culprit?","authors":"Xiaoxin Wu, Zhengyu Zhang, Liang Zhang, David J Daniels","doi":"10.1186/s12985-025-02826-3","DOIUrl":"10.1186/s12985-025-02826-3","url":null,"abstract":"<p><p>Glioblastoma is the most common primary malignant tumor of the central nervous system, with a median survival of less than two years. While the etiology of glioblastoma is unclear, viral infection has emerged as a potential contributing factor. Cytomegalovirus (CMV) was first reported to be associated with glioblastoma in 2002. Since then, many studies have detected CMV in glioblastoma tissues suggesting it may plays a role in the glioblastoma progression. While there is no direct evidence confirmings CMV as an oncogenic virus, studies have demonstrated that CMV promotes glioblastoma development in cell and animal models, with several CMV-related genes implicated in tumorigenesis. Importantly, adjuvant CMV antiviral therapy has been proven to improve glioblastoma patient survival. This review focuses on clinical studies regarding the relationship between CMV and glioblastoma, the mechanism of CMV in tumorigenesis, advances in animal models of CMV-induced glioblastoma, and key directions for future investigations.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"194"},"PeriodicalIF":4.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplex detection of respiratory RNA viruses without amplification based on CRISPR-Cas13a immunochromatographic test strips.","authors":"Tao Wang, Wenqian Jiang, Zhiqing Huang, Zhitao Yuan, Zhiwei Chen, Jun Lin","doi":"10.1186/s12985-025-02765-z","DOIUrl":"10.1186/s12985-025-02765-z","url":null,"abstract":"<p><p>Acute respiratory infections, caused by RNA viruses like respiratory syncytial virus, influenza, rhinovirus, and coronavirus, are major global health threats. Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) is the gold standard for detecting these viruses but is time-consuming, complex, and requires specialized equipment. There is a need for rapid, convenient, and multi-target detection methods to improve disease prevention and control. This study developed a multi-target immunochromatographic detection method using LbuCas13a protein and \"band elimination\" test strips for detecting SARS-CoV-2 and influenza virus. The method's performance was evaluated by testing known 5 positive and 4 negative samples for SARS-CoV-2 and comparing results with fluorescent PCR and colloidal gold methods. Detection sensitivity was quantified using digital PCR and qPCR. The immunochromatographic test strips showed 100% concordance with fluorescent PCR and colloidal gold methods in initial clinical SARS-CoV-2 detection. Subsequently, we used dual-target immunochromatographic test strips to detect 9 SARS-CoV-2 positive samples and 9 H3N2 positive samples. However, false negatives were observed in dual-target detection of SARS-CoV-2 and H3N2 samples, likely due to low sample concentration or sample degradation. The method had a minimum detection limit of 381.75 copies/µL, as determined by digital PCR and qPCR. The developed multi-target immunochromatographic detection method offers a rapid, low-cost, and simple approach for detecting both SARS-CoV-2 and influenza viruses. With high sensitivity, specificity, and reliability, this method holds promise as a practical tool for RNA virus diagnosis and improving public health response to respiratory infections.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"192"},"PeriodicalIF":4.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: a critically ill patient with aggressive NK-cell leukemia receiving emergency chemotherapy in the ICU.","authors":"Yuqing Zeng, Yuanyuan Yang, Jian Huang, Haitao Liu, Lina Quan, Xiuwen Lan","doi":"10.1186/s12985-025-02798-4","DOIUrl":"10.1186/s12985-025-02798-4","url":null,"abstract":"","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"193"},"PeriodicalIF":4.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lamin A/C promotes HBV transcription by modulating histone modification associated with cccDNA minichromosome in an HBx-dependent manner.","authors":"Linshan Jiang, Lanlang Peng, Hong Chen, Fangli Liao, Liping Yang, Yuanyuan Ye, Gaoli Zhang, Hua Sun, Qin Hu, Weixian Chen","doi":"10.1186/s12985-025-02820-9","DOIUrl":"10.1186/s12985-025-02820-9","url":null,"abstract":"<p><strong>Background: </strong>Chronic hepatitis B virus (HBV) infection remains a serious public health challenge, and primary treatment strategies are unable to cure HBV infection due to the persistence of HBV covalently closed circular DNA (cccDNA) in the nuclei of cells. The HBV X protein (HBx) plays a crucial role in regulating cccDNA transcription. Therefore, targeting HBx to identify host proteins that regulate cccDNA transcription could represent a curative approach.</p><p><strong>Methods: </strong>Here, we used co-immunoprecipitation (co-IP) and mass spectrometry (MS) to identify proteins that interact with HBx. The candidate proteins Lamin A and C (Lamin A/C) were knocked down by RNA interference, and Lamin A/C was overexpressed via lentiviral vectors in HBV-infected cell cultures. Chromatin immunoprecipitation (ChIP) followed by quantitative PCR (ChIP-qPCR) was used to investigate protein-DNA interactions.</p><p><strong>Results: </strong>A mechanistic study revealed that Lamin A/C bound to HBx, resulting in reduced degradation of HBx by the 26S proteasome. Moreover, Lamin A/C regulated HBV cccDNA transcription in vitro, which was associated with HBx. In addition, Lamin A/C knockdown resulted in transcriptional silencing of the HBV cccDNA but not in HBV-HBx-deficient (∆HBx)-infected cells. Importantly, experimental knockdown of Lamin A/C reduced the level of active histone modifications (H3K4me3 and H3k27Ac) bound to cccDNA and increased the level of inhibited histone modifications (H3K9me3 and H3K27me3) bound to cccDNA, which was suppressed in the HBV-∆HBx system.</p><p><strong>Conclusion: </strong>Our findings reveal that Lamin A/C acts as a new host factor for HBV cccDNA through an epigenetic regulatory mechanism that can interact with HBx and prevent its degradation.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"189"},"PeriodicalIF":4.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}