Isolation and genetic characterization of parvovirus from Bengal tiger in China.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2025-10-10 DOI:10.1186/s12985-025-02952-y

Ye Wang, Shuyi Han, Yi Li, Guohui Yuan, Yanan Xing, Wanqian Chen, Mengyan Cheng, Hongxuan He

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引用次数: 0

Abstract

Background: Canine parvovirus (CPV) poses an emerging threat to endangered felids, yet its epidemiology and evolutionary dynamics in tigers remain poorly characterized.

Methods: Pathogen detection was performed through necropsy, colloidal gold assays, and PCR on a deceased Bengal tiger, followed by genetic and molecular characterization of the identified CPV.

Results: This study isolated a CPV-2c strain (HNSY-031) from captive Bengal tigers. Genetic analysis revealed its closest relationship to Vietnamese canine parvoviruses, with 100% identity to CPV strains circulating in China and Vietnam since 2020. Molecular characterization identified 12 amino acid mutations within the VP2 protein, including key residues associated with host adaptation and viral evolution.

Conclusions: The isolated CPV-2c strain may have originated from multiple potential sources, including contact with domestic or wild animals or contaminated environments. These findings highlight the necessity of implementing comprehensive biosecurity protocols around wildlife facilities, expanding vaccination programs for animals in surrounding regions, and establishing ongoing surveillance systems to monitor potential sources of pathogen exposure.

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中国孟加拉虎细小病毒的分离及遗传特性分析。

背景：犬细小病毒（Canine parvovirus， CPV）对濒危猫科动物构成了新的威胁，但其在老虎中的流行病学和进化动力学特征尚不清楚。方法：对死亡的孟加拉虎进行尸检、胶体金测定和PCR检测病原体，随后对鉴定的CPV进行遗传和分子鉴定。结果：本研究从圈养孟加拉虎中分离出CPV-2c毒株HNSY-031。遗传分析显示其与越南犬细小病毒的亲缘关系最密切，与2020年以来在中国和越南流行的CPV毒株100%同源。分子鉴定鉴定出VP2蛋白内的12个氨基酸突变，包括与宿主适应和病毒进化相关的关键残基。结论：分离的CPV-2c菌株可能来源于多种潜在来源，包括与家养或野生动物或污染环境的接触。这些发现强调了在野生动物设施周围实施综合生物安全协议、扩大周边地区动物疫苗接种规划以及建立持续监测系统以监测潜在病原体暴露源的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.