Virology Journal最新文献

{"title":"Human papillomavirus infection in males: implications for fertility, assisted reproductive outcomes, and vaccination strategies.","authors":"Xian-Hua Sun, Yu-Hua Lan, Nan Wang","doi":"10.1186/s12985-025-02833-4","DOIUrl":"10.1186/s12985-025-02833-4","url":null,"abstract":"<p><p>Human papillomavirus (HPV), a prevalent sexually transmitted infection, is increasingly recognized as a critical determinant of male reproductive dysfunction. This comprehensive review consolidates evidence on HPV-mediated mechanisms impairing semen quality, inducing sperm DNA fragmentation, and facilitating paternal-fetal transmission, which collectively contributes to spontaneous abortion and assisted reproductive technology (ART) failure. While debates persist on the direct causal role of HPV in infertility, emerging data highlight the efficacy of male vaccination in reducing the seminal viral load, improving pregnancy rates, and lowering miscarriage risk. We propose integrating HPV screening into male fertility assessments and advocating gender-neutral vaccination programs to increase ART success and mitigate vertical transmission. Future research should delineate HPV-induced epigenetic alterations and validate novel sperm preparation techniques for optimizing reproductive outcomes.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"209"},"PeriodicalIF":4.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12205511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot one-step detection platform for severe fever with thrombocytopenia syndrome virus via the CRISPR/Cas12a detection system.","authors":"Jiwei Shu, Qilong Tan, Zhe Huang, Tongjie Zhang, Ling Ye, Shuqin Fu, Zhilei Mao","doi":"10.1186/s12985-025-02772-0","DOIUrl":"10.1186/s12985-025-02772-0","url":null,"abstract":"<p><p>Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne virus that primarily causes SFTS. Although a common testing route is available, a timely, conventional and accurate method for SFTSV detection is urgently needed. In the present study, we established a platform that combines the recombinase polymerase amplification (RPA) assay with the clustered regularly interspaced short palindromic repeats-CRISPR associated proteins (CRISPR/Cas) 12a technique in one step in one pot. The procedure can be completed within 45 min at a constant temperature without a sophisticated instrument. This method targets the S gene of SFTSV, with a detection limit (LoD) of 11.7 copies per reaction and high specificity, without cross reactivity with other pathogens. Furthermore, across 46 test samples, this method achieved 89.13% consistency with the PCR method (41/46). Together, the reaction system developed in the present study provides not only a novel method for SFTSV detection but also an alternative method for detecting RNA viruses.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"203"},"PeriodicalIF":4.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of four commercial analyzers for the serological screening of hepatitis A.","authors":"Junhyup Song, Jiyeon Kim, Sinyoung Kim, Younhee Park","doi":"10.1186/s12985-025-02770-2","DOIUrl":"10.1186/s12985-025-02770-2","url":null,"abstract":"<p><strong>Background: </strong>Serological assays for hepatitis A virus (HAV) play a crucial role in diagnosing acute infections and monitoring disease transmission. Given their widespread use in clinical laboratories, discrepancies among different immunoassay analyzers may have significant clinical implications. This study aimed to assess the quantitative and qualitative agreement between anti-HAV total immunoglobulin (or IgG) and IgM results across four fully automated immunoassay systems.</p><p><strong>Methods: </strong>A total of 280 and 223 clinical serum samples were tested for anti-HAV total immunoglobulin (or IgG) and IgM, respectively, using four immunoanalyzers: Vitros ECiQ (Ortho), Atellica IM 1600 (Siemens), Alinity i (Abbott), and Cobas e801 (Roche). Quantitative correlations and qualitative agreements were assessed, and cases with discordant anti-HAV IgM results were further investigated using available clinical data.</p><p><strong>Results: </strong>While the total immunoglobulin (or IgG) assay demonstrated a strong correlation across all platforms, substantial discrepancies were observed in the IgM results, particularly between the Vitros ECiQ and the other three analyzers. Although the other three platforms yielded concordant results, the clinical review indicated that in 4 out of 6 cases (66.6%), the Vitros ECiQ results aligned more closely with the clinical presentations.</p><p><strong>Conclusions: </strong>This study highlights inter-assay variability in anti-HAV IgM detection and underscores the need for improved harmonization across platforms. Future studies incorporating a sufficient number of molecularly confirmed acute hepatitis A cases are warranted to clarify the causes of false results and minimize the potential clinical impact of inaccurate testing.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"207"},"PeriodicalIF":4.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus.","authors":"Jianzhong Wang, Jicheng Qiu, Mengmeng Wang, Xiaojie Wu, Xiaoguang Li, Heng Zhang","doi":"10.1186/s12985-025-02815-6","DOIUrl":"10.1186/s12985-025-02815-6","url":null,"abstract":"<p><p>Equine infectious anemia virus (EIAV) poses a significant global threat to the equine industry, resulting in considerable economic losses and compromised horse health. Given the limited treatment options available, early detection and prevention strategies are essential to combat this viral infection. In this study, we introduce a novel colloidal gold immunochromatographic (ICLF) strip for the rapid and accurate detection of EIAV antibodies. The strip incorporates a unique fusion protein, p26-gp90, designed to enhance both the specificity and sensitivity of the detection method for EIAV antibodies. Through rigorous method validation, our strip has exhibited superior performance compared to existing detection methods. It demonstrates high sensitivity, improved specificity, and excellent stability, enabling reliable and long-term storage. Additionally, the strip allows for fast and precise analysis of clinical samples, making it an invaluable tool for the early detection and prevention of EIAV. The availability of such an accurate and efficient detection method will greatly enhance our ability to combat EIAV by enabling the early identification of infected horses and facilitating effective preventive measures.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"205"},"PeriodicalIF":4.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of convalescent plasma therapy in improving survival in non-immunized COVID-19 patients.","authors":"Tania Portella Costa, Dayanne Mozaner Bordin, Mateus Nóbrega Aoki, Lucas Blanes","doi":"10.1186/s12985-025-02778-8","DOIUrl":"10.1186/s12985-025-02778-8","url":null,"abstract":"<p><strong>Purpose: </strong>Convalescent plasma (CP), obtained from individuals who have recovered from COVID-19, has been widely explored as a potential therapeutic option, particularly in the absence of vaccines and monoclonal antibody treatments. This study aimed to evaluate the effectiveness of CP therapy in improving survival among non-immunized COVID-19 patients hospitalized in Brazil.</p><p><strong>Methods: </strong>This retrospective unicentric cohort study was conducted at a private hospital in Campo Largo, Paraná, Brazil, from July 2020 to February 2021. A total of 245 hospitalized COVID-19 patients were included, confirmed by RT-qPCR or antigen testing. Patients were divided into two groups: those receiving CP alongside standard treatment (n=100) and those receiving standard treatment alone (n=145). Survival outcomes were assessed using Kaplan-Meier analysis and Cox regression, while inflammatory responses were evaluated through C-reactive protein (CRP) measurements.</p><p><strong>Results: </strong>Patients treated with CP had a significantly higher survival rate (91%) compared to the control group (82.8%) (P=0.0363). The survival benefit persisted throughout the follow-up period, with a 2.25-fold lower risk of death in the CP group after adjusting for age (P=0.0480). However, no significant differences in CRP levels were observed between groups at discharge, suggesting that CP's benefits may be mediated through immune modulation rather than direct anti-inflammatory effects.</p><p><strong>Conclusions: </strong>Our findings indicate that CP therapy significantly improves survival in non-immunized COVID-19 patients, reinforcing its potential role in settings with limited access to advanced treatments. Future studies should explore CP's mechanisms of action and its integration into broader therapeutic strategies.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"206"},"PeriodicalIF":4.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating enterovirus diversity among symptomatic patients in Hungary during and after easing the COVID-19 lockdown.","authors":"Nóra Deézsi-Magyar, Gyula Zsidei, Norbert Kiss, Bereniké Novák, Marianna Mezősi-Csaplár, Katalin Réka Tarcsai, Adrienne Lukács, Erzsébet Barcsay, Katalin Szomor, Mária Takács","doi":"10.1186/s12985-025-02835-2","DOIUrl":"10.1186/s12985-025-02835-2","url":null,"abstract":"<p><strong>Background: </strong>The COVID-19 pandemic led to widespread public health interventions that significantly affected the transmission of various pathogens, including enteroviruses (EVs). EVs exhibit considerable genetic diversity and can cause clinical manifestations ranging from mild illnesses to severe diseases. Our present study aimed to evaluate the diversity of circulating EV types in Hungary and assess the impact of lockdown measures on EV prevalence based on testing clinical samples obtained from symptomatic patients.</p><p><strong>Methods: </strong>As part of the routine enterovirus diagnosis, we conducted quantitative reverse transcription polymerase chain reaction (RT-qPCR) on clinical samples obtained from patients presenting with symptoms corresponding to EV infection. Positive samples were then subjected to virus isolation in cell culture and next-generation sequencing (NGS). Phylogenetic analysis was performed to place the newly generated sequences within the global diversity of EV strains for comparison.</p><p><strong>Results: </strong>During this period, an overall number of 125 patients tested positive for EVs, mostly children under the age of 15 years. The most common symptoms were fever, hand-foot-mouth disease, encephalitis, and meningitis. The temporal distribution of EV-positive cases showed strong seasonality, with peaks in the summer and autumn months. The lowest number of confirmed cases occurred during the lockdown years, attributed to limited sample collection and reduced personal contacts. However, following the easing of restrictions, the number of cases significantly increased, with the highest incidence observed in 2022. The distribution of EV genotypes shifted notably after easing the lockdowns. While only coxsackievirus (CV)A6 was detected during 2021, a broader range of genotypes emerged afterwards, including CVA10, CVA16, echovirus E9, and E11.</p><p><strong>Conclusions: </strong>Next-generation sequencing analysis revealed notable genotypic diversity, providing valuable insights into the evolution of EVs in Hungary and across Europe. These findings underscore the importance of continued surveillance of enterovirus infections, particularly in the context of pandemic recovery, as the shifting EV genotype landscape may impact disease severity and spread, highlighting the need for adaptive public health responses.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"204"},"PeriodicalIF":4.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear keratin 6A upregulates human papillomavirus oncogene expression through TEAD1 interaction.","authors":"Tomoya Miyamura, Seiichiro Mori, Mamiko Onuki, Akihiko Sekizawa, Koji Matsumoto, Iwao Kukimoto","doi":"10.1186/s12985-025-02832-5","DOIUrl":"10.1186/s12985-025-02832-5","url":null,"abstract":"","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"202"},"PeriodicalIF":4.0,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an indirect enzyme-linked immunosorbent assay based on nucleocapsid protein for the detection of swine acute diarrhea syndrome coronavirus antibody.","authors":"Yu-Jeong Jang, Nam Phuong Le, Eun-Song Lee, Min-Chae Kim, Tae-Kyung Chang, Jung-Eun Park","doi":"10.1186/s12985-025-02834-3","DOIUrl":"10.1186/s12985-025-02834-3","url":null,"abstract":"<p><strong>Background: </strong>Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a porcine enteric coronavirus that induces watery diarrhea in pigs, causing substantial economic losses in the swine industry. While the molecular and serological epidemiology of SADS-CoV in China has been extensively studied, comprehensive epidemiological studies assessing its prevalence outside China are lacking.</p><p><strong>Results: </strong>In this study, an indirect enzyme-linked immunosorbent assay based on the SADS-CoV N protein (N-iELISA) was developed to evaluate the seroprevalence of SADS-CoV in Korean pig herds. The optimal conditions for N-iELISA were determined through checkerboard titration of serum samples verified via western blotting. The assay showed sufficient specificity and reproducibility, with a cutoff value of 0.484. A total of 540 field samples collected from pig herds across nine provinces in Korea were subsequently tested using the N-iELISA. The findings revealed an overall seroprevalence of SADS-CoV in Korea of 8.70%.</p><p><strong>Conclusions: </strong>These results indicate that the N-iELISA is a reliable tool for seroepidemiological studies of SADS-CoV and suggest that the seroprevalence of SADS-CoV in the Korean pig population is relatively low.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"201"},"PeriodicalIF":4.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Characterization of the epidemiology, susceptibility genes and clinical features of viral infections among children with inborn immune errors: a retrospective study.","authors":"Haiqiao Zhang, Wenjie Wang, Qinhua Zhou, Jia Hou, Wenjing Ying, Xiaoying Hui, Jinqiao Sun, Lipin Liu, Luyao Liu, Chenhao Wang, Hai Zhang, Bijun Sun, Xiaochuan Wang","doi":"10.1186/s12985-025-02808-5","DOIUrl":"10.1186/s12985-025-02808-5","url":null,"abstract":"","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"200"},"PeriodicalIF":4.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the genetic diversity of the 3' untranslated region (UTR) of Dengue virus.","authors":"Alessandra Lo Presti, Arnold Knijn, Enrico Palermo, Alessandra D'Auria, Miriana Quaranta, Stefano Pascarella, Federica Frasca, Carolina Scagnolari","doi":"10.1186/s12985-025-02800-z","DOIUrl":"10.1186/s12985-025-02800-z","url":null,"abstract":"<p><strong>Background: </strong>Dengue virus (DENV), with its four antigenically distinct serotypes, is the etiological agent of dengue fever, which is endemic in tropical and subtropical regions but has recently spread to previously non-endemic areas such as Europe. Given the growing body of evidence suggesting that sequence diversification in the 3' UTR of DENV contributes to its epidemiological fitness and host adaptation, we conducted phylogenetic and genetic diversity analyses on four DENV 3' UTR datasets (DENV-1, DENV-2, DENV-3 and DENV-4).</p><p><strong>Methods: </strong>A maximum likelihood phylogenetic analysis was performed using IQ-TREE, and the average evolutionary divergence was estimated using MEGA X. Maximum likelihood analysis combined with genetic distance calculations provided insight into the evolutionary dynamics of the DENV 3' UTR.</p><p><strong>Results: </strong>A higher number of supported internal clusters/clades were found in the DENV-2 and DENV-1 3' UTR trees, probably indicating strains with similar evolutionary histories. In terms of cluster composition, apart from a general mixing of DENV 3' UTR sequences from different sites and years, the majority of supported internal clusters were composed of sequences aggregated according to their location. Genetic distances showed that the DENV-1 3' UTR has a higher variability (5%) compared to DENV-2 (3%), DENV-3 and DENV-4 (2%). The average length of the 3' UTR, as estimated from our datasets, showed that it was longest in DENV-2, followed by DENV-3, DENV-1 and DENV-4.</p><p><strong>Conclusions: </strong>In conclusion, this study provided a comprehensive analysis of 3' UTR evolution and phylogenies in all four DENV serotypes, suggesting that this viral genomic sequence is subject to genetic variability and length changes.</p>","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"197"},"PeriodicalIF":4.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12178008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}