Development of an indirect enzyme-linked immunosorbent assay based on nucleocapsid protein for the detection of swine acute diarrhea syndrome coronavirus antibody.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2025-06-20 DOI:10.1186/s12985-025-02834-3

Yu-Jeong Jang, Nam Phuong Le, Eun-Song Lee, Min-Chae Kim, Tae-Kyung Chang, Jung-Eun Park

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引用次数: 0

Abstract

Background: Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a porcine enteric coronavirus that induces watery diarrhea in pigs, causing substantial economic losses in the swine industry. While the molecular and serological epidemiology of SADS-CoV in China has been extensively studied, comprehensive epidemiological studies assessing its prevalence outside China are lacking.

Results: In this study, an indirect enzyme-linked immunosorbent assay based on the SADS-CoV N protein (N-iELISA) was developed to evaluate the seroprevalence of SADS-CoV in Korean pig herds. The optimal conditions for N-iELISA were determined through checkerboard titration of serum samples verified via western blotting. The assay showed sufficient specificity and reproducibility, with a cutoff value of 0.484. A total of 540 field samples collected from pig herds across nine provinces in Korea were subsequently tested using the N-iELISA. The findings revealed an overall seroprevalence of SADS-CoV in Korea of 8.70%.

Conclusions: These results indicate that the N-iELISA is a reliable tool for seroepidemiological studies of SADS-CoV and suggest that the seroprevalence of SADS-CoV in the Korean pig population is relatively low.

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基于核衣壳蛋白间接酶联免疫吸附法检测猪急性腹泻综合征冠状病毒抗体的建立。

背景：猪急性腹泻综合征冠状病毒（SADS-CoV）是一种引起猪水样腹泻的猪肠道冠状病毒，给养猪业造成了巨大的经济损失。虽然对SADS-CoV在中国的分子和血清学流行病学进行了广泛的研究，但缺乏评估其在中国以外流行情况的全面流行病学研究。结果：本研究建立了基于SADS-CoV N蛋白的间接酶联免疫吸附法（N- ielisa），用于评价韩国猪群SADS-CoV血清阳性率。血清样品经western blotting验证，通过棋盘滴定法确定N-iELISA的最佳条件。该方法具有足够的特异性和重复性，截止值为0.484。随后使用N-iELISA对从韩国9个道的猪群中收集的540个现场样本进行了检测。结果显示，韩国SADS-CoV的总体血清阳性率为8.70%。结论：N-iELISA是一种可靠的SADS-CoV血清流行病学研究工具，在韩国猪群中SADS-CoV血清流行率相对较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.