Veterinary parasitology, regional studies and reports最新文献

{"title":"The first morphological and molecular characterization of wing louse (Lipeurus caponis) from ornamental and indigenous chickens of West-Coast India","authors":"Shirish Dadarao Narnaware,&nbsp;Nibedita Nayak,&nbsp;Prasastha Vemula,&nbsp;Susitha Rajkumar,&nbsp;Amiya Ranjan Sahu","doi":"10.1016/j.vprsr.2025.101307","DOIUrl":"10.1016/j.vprsr.2025.101307","url":null,"abstract":"<div><div>Lice infestation in poultry presents a substantial challenge to poultry farmers, with adverse implications for bird health, welfare, and productivity. In this study, the outbreak of wing louse infestation was recorded in ornamental and improved indigenous chicken varieties of an organized poultry farm located in the west-coast of India during the winter season. The lice were found to be attached to the underside of the wings between the feather barbs on the primary or secondary wing feathers. In addition to the active lice population, white clumps of lice's eggs (nits) were found attached at the base of feathers in the breast and thigh region. The affected birds showed signs of feather loss, irritation, itching, annoyance, restlessness, scratching, and feather plucking. Morphologically, the adult lice were dark brown colored, wingless, and elongated with dorso-ventrally flattened bodies. The female lice were significantly larger than the males in body length, post-antennal head width, thorax width, abdominal width, and abdominal length. The body structure resembled <em>Lipeurus caponis</em> when observed under a stereo microscope. For molecular characterization, the total genomic DNA extracted from whole lice was used to amplify the mitochondrial COI (Cytochrome Oxidase Subunit I) gene using universal primers. The PCR product obtained was sequenced, and the gene sequence was submitted to GenBank. Based on the phylogenetic analysis of the COI gene, of the present study's sequences were clustered with <em>L. caponis</em> hosted by Indian peacocks of China and Jungle fowl of the UK. This is the first record of the COI sequence of <em>L. caponis</em> from India.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101307"},"PeriodicalIF":1.4,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceptions and interventions concerning coccidiosis in Dutch sheep farms","authors":"Mark van der Heijden ,&nbsp;Snorre Stuen ,&nbsp;Inge Santman-Berends ,&nbsp;Carlijn ter Bogt-Kappert ,&nbsp;Caroline Verduijn ,&nbsp;Piet Vellema","doi":"10.1016/j.vprsr.2025.101306","DOIUrl":"10.1016/j.vprsr.2025.101306","url":null,"abstract":"<div><div>Coccidiosis in sheep is a protozoan disease of young lambs that is not yet fully understood, and its control still largely relies on the use of coccidiostats, despite development of resistance. It is hypothesized that in the Netherlands a proper diagnosis is not always made before treatment is initiated, possibly leading to an overuse of coccidiostats. The aims of this study are to investigate the perceptions and interventions of Dutch sheep farmers and their veterinarians regarding coccidiosis, with special attention to treatment and the use of anticoccidial drugs. An online survey was completed by 114 sheep farmers and 57 veterinary practitioners. The surveyed sheep farmers appeared to be genuinely interested in coccidiosis, with three-quarters considering it an important problem in their flock. Seventy per cent of the sheep farmers and 84 % of the veterinarians reported diarrhoea as the most important symptom of coccidiosis. Almost three-quarters of sheep farmers conducted faecal examination during the two preceding years, 87 % per cent have performed treatments, and half of them used coccidiostats annually. There was a wide variety of treatment approaches. Standard treatment of lambs without any clinical indication, or before 4 weeks of age, and treatment of adult sheep were reported by 26 %, 13 %, and 13 % of responding farmers, respectively, and the latter two are considered as incorrect treatments. In contrast, veterinarians estimated that, on average, coccidiostats are prescribed on 30 % of sheep farms in their practice, with treatment primarily based on clinical signs and faecal examination results. Potential obstacles to a correct diagnosis included practical difficulties in collecting faecal samples, and additional costs. Remarkably, questionable treatments were common, including treatments without a history of coccidiosis, treatments of lambs younger than one month of age and treatments of adult sheep. Although correct dosage of coccidiostats is crucial, 43 % of veterinarians advised dosing based on the heaviest animals, while in most cases weight was visually estimated by both farmer and veterinarian. Unfortunately, treatments were frequently applied without a proper diagnosis, and the applied dosage was often not correct, which may lead to unnecessary and incorrect use of coccidiostats resulting in an increased risk of resistance development. Since veterinarians are regarded as the most important advisors, they play a key role in the solution. By supporting veterinarians, preventive measures and more prudent use of coccidiostats can be promoted.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101306"},"PeriodicalIF":1.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution to an efficient diagnosis of Fasciola hepatica infection in naturally infected cattle","authors":"Natã Medeiros Godinho ,&nbsp;Camila Zomer Spíndola ,&nbsp;Márcia Sangaletti Lavina ,&nbsp;Andreas Lazaros Chryssafidis ,&nbsp;Anderson Barbosa de Moura","doi":"10.1016/j.vprsr.2025.101301","DOIUrl":"10.1016/j.vprsr.2025.101301","url":null,"abstract":"<div><div>The control of fascioliasis caused by <em>Fasciola hepatica</em> depends on early detection and assessment of infection severity. A definitive diagnosis is the post-mortem recovery of flukes in the hepatic ducts and gallbladder, which is not feasible for herd monitoring. Coproparasitological techniques based on egg detection in feces are commonly used. This study evaluated the accuracy of the Dennis, Stone, and Swanson (DSS), quatro tamises (QT), and Gordon and Whitlock modified (GWM) techniques using parasitized livers as the gold standard. Of 784 carcasses, 105 (13.39 %) were parasitized by <em>F. hepatica</em>. The sensitivity was 43.8 % for DSS, 86.6 % for QT, and 7.6 % for GWM. In comparison with the gold standard, agreement on the Kappa scale was weak for GWM (0.10), moderate for DSS (0.45), and excellent for QT (0.84). Additionally, when comparing the techniques with each other, modest agreement was observed between QT and GWM (0.20), and moderate agreement between DSS and GWM (0.57) and DSS and QT (0.48). Parasite intensity did not influence (<em>P</em> &gt; 0.05) the sensitivity of DSS and QT. These findings indicate that QT is the most sensitive and reliable coproparasitological technique for diagnosing <em>F. hepatica</em> infection in naturally infected cattle.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101301"},"PeriodicalIF":1.4,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, geospatial distribution, and risk factors for bovine cysticercosis across diverse states of Brazil","authors":"Fabio Martins Guerra Nunes Dias ,&nbsp;Laura Lúcia dos Santos Oliveira ,&nbsp;Fredson Vieira e Silva ,&nbsp;André Guimarães Maciel e Silva ,&nbsp;José Bento Sterman Ferraz ,&nbsp;Guilherme Jordão de Magalhães Rosa","doi":"10.1016/j.vprsr.2025.101305","DOIUrl":"10.1016/j.vprsr.2025.101305","url":null,"abstract":"<div><div>This study examined the prevalence and spatial distribution of bovine cysticercosis in Brazil between 2018 and 2020, focusing on its associations with factors such as the human development index (HDI), population density, cattle weight, age, and sex. The analysis included data from approximately 22 million bovine carcasses from 10 of Brazil's 26 states, revealing an overall carcass prevalence of 0.09 %. Although this prevalence is considered low, it varies significantly across different states and microregions. The geospatial study of bovine cysticercosis identified clusters with high prevalence (high-high) and outliers (high-low) in Brazilian microregions. The study revealed significant associations between HDI, population density, and cattle age and the prevalence of cysticercosis; however, the models lacked robustness. In Brazilian microregions, these findings emphasize the need for targeted interventions and intensified surveillance in higher-risk areas, reinforcing regional variations and supporting the development of more effective public policies and health interventions.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101305"},"PeriodicalIF":1.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importation of the exotic tick Amblyomma geoemydae into Sweden via illegally introduced turtles (Geoemyda spengleri) from Thailand","authors":"Giulio Grandi ,&nbsp;Anna Omazic ,&nbsp;Cesar Guevara ,&nbsp;Anton de Jong ,&nbsp;Lidia Chitimia-Dobler","doi":"10.1016/j.vprsr.2025.101304","DOIUrl":"10.1016/j.vprsr.2025.101304","url":null,"abstract":"<div><div>Illegal importation of animals as pets might be followed by the introduction of exotic tick species, potential vectors of pathogens that can have an impact on wildlife and/or domestic animals and on humans. Therefore, permanent measures of surveillance and control on imported reptiles are emphasized. In February 2024, black-breasted leaf turtles (<em>Geoemyda spengleri</em>) (<em>n</em> = 31) were illegally imported into Sweden from Thailand. The turtles were sent by the Swedish Customs Criminal Department West in Gothenburg to a public aquarium and terrarium (Tropicarium, Kolmården, Östergötland county) to be kept in quarantine. At the Tropicarium the turtles were found to be infested by ticks. All ticks (<em>n</em> = 3) found were removed and stored in ethanol. The finding (including photos of the ticks) was reported to the Swedish Veterinary Agency (SVA, Uppsala) using the Report Tick Tool, a surveillance system developed by the SVA itself. The three collected ticks were identified based on morphology and genetics as <em>Amblyomma geoemydae</em> nymphs. This discovery demonstrates that SVA's surveillance tool can function as an early warning system for newly introduced tick species and to promptly identify exotic tick species which can carry viruses, bacteria, and/or parasites that are not currently present in the country.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101304"},"PeriodicalIF":1.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular evidence of Aelurostrongylus abstrusus in domestic cats from Africa and a synopsis of its occurrence across the continent","authors":"Ioana Bianca Mitrea ,&nbsp;Petr Cibulka ,&nbsp;Angela Monica Ionică ,&nbsp;Nonzuzo Audrey Ndlovu ,&nbsp;Norman Leo Mukarati ,&nbsp;Thokozani Hove ,&nbsp;Noureddine Mechouk ,&nbsp;Mihai Sorin Cernea ,&nbsp;Andrei Daniel Mihalca","doi":"10.1016/j.vprsr.2025.101302","DOIUrl":"10.1016/j.vprsr.2025.101302","url":null,"abstract":"<div><div>Domestic cats (<em>Felis catus</em>) can be infected with a variety of cardio-pulmonary nematodes. Although <em>Aelurostrongylus abstrusus</em> is widely distributed globally, reports from Africa are scarce and refer mainly to wild felids. Moreover, some of these reports are considered as misidentifications. The only report in domestic cats in Africa is more than 20 years old and originated from Kenya. The aim of this study was to evaluate the presence of <em>A. abstrusus</em> in domestic cats from Harare, Zimbabwe morphologically on light microscopy and confirmation by molecular means.</div><div>A total of 57 domestic cats were included in this study, and fecal samples were analyzed using the Baermann technique. The detected larvae were collected, morphologically identified, and subsequently confirmed through molecular analysis by targeting three genes.</div><div>One sample out of 57 tested positive for first-stage larvae (L1) and was identified as <em>Aelurostrongylus abstrusus</em> based on morphological characteristics. Molecular analysis subsequently confirmed the species identity.</div><div>The present study is the first molecular confirmation of <em>Aelurostrongylus abstrusus</em> in domestic cats from Africa and the first case in domestic cats from southern Africa. Future studies are recommended to further investigate its distribution and epidemiology.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101302"},"PeriodicalIF":1.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New registers of Ixodidae ticks on Cervidae from Santa Catarina Plateau, Brazil","authors":"Luisa Barreto Rippel ,&nbsp;Sara Santos de Mello ,&nbsp;Anderson Barbosa de Moura ,&nbsp;Thiago Fernandes Martins ,&nbsp;Andreas Lazaros Chryssafidis","doi":"10.1016/j.vprsr.2025.101299","DOIUrl":"10.1016/j.vprsr.2025.101299","url":null,"abstract":"<div><div>This study aimed to identify ixodid tick species parasitizing wild cervids referred to a wildlife rehabilitation center located in the Santa Catarina Plateau, southern Brazil, a region characterized by its inclusion in the Atlantic Forest biome and its unique ecological features. Five wild cervids of different species were examined for ectoparasites during clinical evaluation. A total of 28 ixodid ticks, representing various developmental stages, were collected using forceps, preserved in 70 % ethanol, and morphologically identified. The most prevalent species was <em>Rhipicephalus microplus</em>, followed by <em>Haemaphysalis juxtakochi</em> and <em>Ixodes fuscipes</em>. The identification of these tick species, some of which are recognized as vectors of zoonotic and veterinary-relevant pathogens, underlines the potential risks associated with tick-borne diseases in fragmented forest areas. These findings reinforce the importance of systematic surveillance of ectoparasites in wildlife populations to support conservation efforts and contribute to public and animal health strategies.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101299"},"PeriodicalIF":1.4,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Year-round serological and molecular survey implies the spread of the re-emerged zoonotic canine Leishmania infantum from mountainous hotspot to the lowland in Beijing","authors":"Yujuan Chen ,&nbsp;Han Tang ,&nbsp;Yonglan Yu","doi":"10.1016/j.vprsr.2025.101300","DOIUrl":"10.1016/j.vprsr.2025.101300","url":null,"abstract":"<div><div>Canine leishmaniasis, caused by <em>Leishmania infantum</em>, poses significant zoonotic risks, particularly in endemic regions. This study conducted a year-round serological and molecular survey across 16 districts in Beijing, China, from March 2021 to February 2022, aiming to investigate the prevalence and distribution of L. <em>infantum</em> among domestic dogs. A total of 575 canine blood samples were analyzed using enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR), with 6.3 % testing positive (i.e., ELISA and/or qPCR positive). Infection rates varied geographically, with the highest positivity rate observed in the mountainous Mentougou District (50.0 %), suggesting a potential hotspot and source of disease spread to adjacent lowland urban areas. Risk factor analysis revealed that infection was significantly associated with age and season, with younger dogs (&lt;6 years) and samples collected during spring showing higher positivity rates (14.1 % and 20.3 %, respectively). Gender and breed were not significant predictors. Phylogenetic analysis of ITS2 sequences confirmed the presence of L. <em>infantum</em>, and microscopic examination further corroborated the findings. These results underscore the importance of combined serological and molecular diagnostics for accurate detection. The observed geographical and seasonal trends suggest an urgent need for enhanced surveillance and targeted control measures to mitigate the public health risks posed by L. <em>infantum</em> in this rapidly urbanizing region. This study provides critical insights into the epidemiology of canine leishmaniasis in Beijing, emphasizing the role of environmental factors and reservoir hosts in disease dynamics.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101300"},"PeriodicalIF":1.4,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted next-generation sequencing reveals hemotropic mycoplasmas, Bartonella spp., and Babesia in shelter dogs from Texas, USA","authors":"Ian Kirimi Daniel ,&nbsp;Maureen A. Kelly ,&nbsp;Emily Orr ,&nbsp;Hassan Hakimi ,&nbsp;Rafael A.N. Ramos ,&nbsp;Meriam N. Saleh ,&nbsp;Jobin J. Kattoor ,&nbsp;Rebecca P. Wilkes ,&nbsp;Guilherme G. Verocai","doi":"10.1016/j.vprsr.2025.101297","DOIUrl":"10.1016/j.vprsr.2025.101297","url":null,"abstract":"<div><div>The screening of vector-borne pathogens (VBPs) in shelter dog populations is crucial for early detection and informs proper management and control efforts. In this study, we used a targeted next-generation sequencing (tNGS) assay to detect VBPs in shelter dogs from Texas. Genomic DNA from whole blood (<em>n</em> = 153) was extracted and subjected to the tNGS assay to search for 15 pathogens. A total of 26.1 % (<em>n</em> = 40/153) of dogs were positive for at least one pathogen, with 80 % (32/40) and 20 % (8/40) samples positive for single and co-infections, respectively. Considering single infections, <em>Mycoplasma haemocanis</em> (13.0 %; <em>n</em> = 20/153) was the most detected pathogen, followed by <em>Bartonella vinsonii</em> subsp. <em>berkhoffii</em> (5.8 %; <em>n</em> = 9/153), and <em>Candidatus</em> Mycoplasma haematoparvum (1.9 %; <em>n</em> = 3/153). Among co-infections, <em>Cand</em>. M. haematoparvum + <em>M. haemocanis</em> (2.6 %; <em>n</em> = 4/153), followed by <em>B. v. berkhoffii</em> + <em>M. haemocanis</em> (1.3 %; <em>n</em> = 2/153), <em>B. v. berkhoffii</em> + <em>Cand</em>. M. haematoparvum (0.7 %; <em>n</em> = 1/153) and <em>B. vulpes</em> + <em>M. haemocanis</em> (0.7 %; n = 1/153). We did not find any association between demographic variables (e.g., age, sex, spayed or neutered status, and breed) and pathogen detection. This study underscores the importance of hemotropic mycoplasma and co-infecting pathogen surveillance in shelter dogs and emphasizes the need for robust and comprehensive assays that allow the simultaneous detection of multiple canine VBPs. Additionally, based on the detection of some pathogens with zoonotic potential, prevention strategies, including comprehensive screenings and targeted biosecurity measures, are recommended for minimizing the risk of exposure to their future owners.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101297"},"PeriodicalIF":1.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the role of Calliphoridae (Insecta: Diptera) as a causative agent of myiasis in sheep with Chrysomya megacephala (Fabricius) as the first documented agent of myiasis in ruminants from Kashmir Himalaya, India","authors":"Tanveer Ahmad Dar,&nbsp;Altaf Hussain Mir","doi":"10.1016/j.vprsr.2025.101298","DOIUrl":"10.1016/j.vprsr.2025.101298","url":null,"abstract":"<div><h3>Purpose</h3><div>Calliphorid flies commonly cause traumatic myiasis in sheep, which refers to the parasitic infestation by fly larvae in living vertebrate tissues and hence poses a great threat to the well-being of vertebrates. In the present study, surveys were carried out in different regions of the Kashmir Himalaya to document the incidence and prevalence caused by myiasis in sheep.</div></div><div><h3>Methods</h3><div>Seasonal surveys were conducted at the selected study locations, especially in the areas with a high density of nomadic herding. The wounds of the sheep were examined for the presence of calliphorid larvae. The fly larvae associated with myiasis were collected from the infested regions with the help of a brush and forceps and preserved in 90 % ethanol for laboratory studies.</div></div><div><h3>Results</h3><div>During the present study, a total of 6441 sheep were screened for the infestation of calliphorids (blowflies) out of which 220 were found harbouring the fly larvae. The ewes (79.09 %) were found more susceptible than males (20.90 %). The incidence and prevalence of myiasis in sheep showed seasonal specificity, with the highest rate in autumn followed by summer, whereas no case of myiasis was recorded in spring and winter. The larvae causing this condition were identified as 3rd instar larvae of <em>Chrysomya megacephala</em> (Fabricius, 1794) <em>and Lucilia sericata</em> (Meigen, 1826). <em>Chrysomya megacephala</em> (Fabricius) has been documented for the first time in India as the causative agent of myiasis in sheep. Predictable mortality rate among the seasons and selected sites was also investigated.</div></div><div><h3>Conclusion</h3><div>The present work is one of the first kind of studies from Kashmir Himalaya compiling information on myiasis-causing flies in sheep, particularly those in the Calliphoridae, and this study will act as a roadmap for further studies on the issue and its management.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101298"},"PeriodicalIF":1.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}