Dogs and cats naturally infected with Trypanosoma cruzi in zoonoses surveillance units from coastal regions of Brazil

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-03-27 DOI:10.1016/j.vprsr.2025.101252

Isabella Neves Aires , Amanda Corvino Valim , Laís Pereira da Silva , Bruna dos Santos Leite , Marcos Vinícius Rangel , Bruna Lindolfo da Silva , João Pessoa Araújo Júnior , Antônio Carlos Paes , Virgínia Bodelão Richini Pereira , Simone Baldini Lucheis

{"title":"Dogs and cats naturally infected with Trypanosoma cruzi in zoonoses surveillance units from coastal regions of Brazil","authors":"Isabella Neves Aires , Amanda Corvino Valim , Laís Pereira da Silva , Bruna dos Santos Leite , Marcos Vinícius Rangel , Bruna Lindolfo da Silva , João Pessoa Araújo Júnior , Antônio Carlos Paes , Virgínia Bodelão Richini Pereira , Simone Baldini Lucheis","doi":"10.1016/j.vprsr.2025.101252","DOIUrl":null,"url":null,"abstract":"<div><div>The Trypanosomatidae family includes protozoa of broad medical and veterinary interest that infect various vertebrates, including humans, and use a range of insects as vector agents. The genus <em>Trypanosoma</em> includes <em>Trypanosoma cruzi</em> (<em>T. cruzi</em>), the etiological agent of Chagas disease, which infects humans and animals, including domestic animals like dogs and cats, which can become infected during the blood meal of triatomine vectors. This study aimed to investigate <em>T. cruzi</em> in 161 blood samples from rescued dogs and cats housed in Zoonoses Surveillance Units (ZSUs) in six municipalities in the coastal region of São Paulo, Brazil. The samples were analyzed using the serological technique of Immunofluorescence Antibody Test (IFAT) and real-time Polymerase Chain Reaction (qPCR) using specific primers for <em>T. cruzi</em>. Spatial visualization through maps showing the distribution of positive cases in the different municipalities studied was also performed. In the IFAT test, three (03) samples from dogs from the municipality of Guarujá were positive for <em>T. cruzi</em> (1.86 %). In the qPCR, 51 samples tested positive for <em>T. cruzi</em> (32.48 %), which belongs to animals from all the municipalities sampled. Thus, investigating <em>T. cruzi</em> in domestic animals from ZSUs enables the identification of potential reservoirs within this environment. Implementing control measures for triatomines, such as installing traps, is essential to epidemiological surveillance in the coastal region of the state of São Paulo.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"60 ","pages":"Article 101252"},"PeriodicalIF":1.4000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology, regional studies and reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405939025000607","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The Trypanosomatidae family includes protozoa of broad medical and veterinary interest that infect various vertebrates, including humans, and use a range of insects as vector agents. The genus Trypanosoma includes Trypanosoma cruzi (T. cruzi), the etiological agent of Chagas disease, which infects humans and animals, including domestic animals like dogs and cats, which can become infected during the blood meal of triatomine vectors. This study aimed to investigate T. cruzi in 161 blood samples from rescued dogs and cats housed in Zoonoses Surveillance Units (ZSUs) in six municipalities in the coastal region of São Paulo, Brazil. The samples were analyzed using the serological technique of Immunofluorescence Antibody Test (IFAT) and real-time Polymerase Chain Reaction (qPCR) using specific primers for T. cruzi. Spatial visualization through maps showing the distribution of positive cases in the different municipalities studied was also performed. In the IFAT test, three (03) samples from dogs from the municipality of Guarujá were positive for T. cruzi (1.86 %). In the qPCR, 51 samples tested positive for T. cruzi (32.48 %), which belongs to animals from all the municipalities sampled. Thus, investigating T. cruzi in domestic animals from ZSUs enables the identification of potential reservoirs within this environment. Implementing control measures for triatomines, such as installing traps, is essential to epidemiological surveillance in the coastal region of the state of São Paulo.

Abstract Image

查看原文本刊更多论文

巴西沿海地区人畜共患病监测单位自然感染克氏锥虫的狗和猫

锥虫科包括具有广泛医学和兽医意义的原生动物，它们感染包括人类在内的各种脊椎动物，并利用一系列昆虫作为媒介媒介。锥虫属包括克氏锥虫（T.克氏锥虫），它是恰加斯病的病原体，可感染人类和动物，包括狗和猫等家畜，它们可在吸食锥虫病媒介的血液时被感染。本研究旨在调查巴西圣保罗沿海地区6个城市人畜共患病监测单位（ZSUs）中获救的狗和猫的161份血液样本中的克氏体。采用免疫荧光抗体试验（IFAT）和实时聚合酶链反应（qPCR）技术对样品进行分析。还通过地图进行了空间可视化，显示了所研究的不同城市中阳性病例的分布。在IFAT测试中，来自瓜鲁杰市的3（03）只狗样本呈克氏体阳性（1.86%）。在qPCR中，51份样本克氏体呈阳性（32.48%），这些样本来自所有采样市的动物。因此，在来自ZSUs的家畜中调查克氏弓形虫，可以在该环境中确定潜在的宿主。实施三角蝽控制措施，如设置陷阱，对圣保罗州沿海地区的流行病学监测至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).