Veterinary parasitology, regional studies and reports最新文献

{"title":"Targeted next-generation sequencing reveals hemotropic mycoplasmas, Bartonella spp., and Babesia in shelter dogs from Texas, USA","authors":"Ian Kirimi Daniel ,&nbsp;Maureen A. Kelly ,&nbsp;Emily Orr ,&nbsp;Hassan Hakimi ,&nbsp;Rafael A.N. Ramos ,&nbsp;Meriam N. Saleh ,&nbsp;Jobin J. Kattoor ,&nbsp;Rebecca P. Wilkes ,&nbsp;Guilherme G. Verocai","doi":"10.1016/j.vprsr.2025.101297","DOIUrl":"10.1016/j.vprsr.2025.101297","url":null,"abstract":"<div><div>The screening of vector-borne pathogens (VBPs) in shelter dog populations is crucial for early detection and informs proper management and control efforts. In this study, we used a targeted next-generation sequencing (tNGS) assay to detect VBPs in shelter dogs from Texas. Genomic DNA from whole blood (<em>n</em> = 153) was extracted and subjected to the tNGS assay to search for 15 pathogens. A total of 26.1 % (<em>n</em> = 40/153) of dogs were positive for at least one pathogen, with 80 % (32/40) and 20 % (8/40) samples positive for single and co-infections, respectively. Considering single infections, <em>Mycoplasma haemocanis</em> (13.0 %; <em>n</em> = 20/153) was the most detected pathogen, followed by <em>Bartonella vinsonii</em> subsp. <em>berkhoffii</em> (5.8 %; <em>n</em> = 9/153), and <em>Candidatus</em> Mycoplasma haematoparvum (1.9 %; <em>n</em> = 3/153). Among co-infections, <em>Cand</em>. M. haematoparvum + <em>M. haemocanis</em> (2.6 %; <em>n</em> = 4/153), followed by <em>B. v. berkhoffii</em> + <em>M. haemocanis</em> (1.3 %; <em>n</em> = 2/153), <em>B. v. berkhoffii</em> + <em>Cand</em>. M. haematoparvum (0.7 %; <em>n</em> = 1/153) and <em>B. vulpes</em> + <em>M. haemocanis</em> (0.7 %; n = 1/153). We did not find any association between demographic variables (e.g., age, sex, spayed or neutered status, and breed) and pathogen detection. This study underscores the importance of hemotropic mycoplasma and co-infecting pathogen surveillance in shelter dogs and emphasizes the need for robust and comprehensive assays that allow the simultaneous detection of multiple canine VBPs. Additionally, based on the detection of some pathogens with zoonotic potential, prevention strategies, including comprehensive screenings and targeted biosecurity measures, are recommended for minimizing the risk of exposure to their future owners.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101297"},"PeriodicalIF":1.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the role of Calliphoridae (Insecta: Diptera) as a causative agent of myiasis in sheep with Chrysomya megacephala (Fabricius) as the first documented agent of myiasis in ruminants from Kashmir Himalaya, India","authors":"Tanveer Ahmad Dar,&nbsp;Altaf Hussain Mir","doi":"10.1016/j.vprsr.2025.101298","DOIUrl":"10.1016/j.vprsr.2025.101298","url":null,"abstract":"<div><h3>Purpose</h3><div>Calliphorid flies commonly cause traumatic myiasis in sheep, which refers to the parasitic infestation by fly larvae in living vertebrate tissues and hence poses a great threat to the well-being of vertebrates. In the present study, surveys were carried out in different regions of the Kashmir Himalaya to document the incidence and prevalence caused by myiasis in sheep.</div></div><div><h3>Methods</h3><div>Seasonal surveys were conducted at the selected study locations, especially in the areas with a high density of nomadic herding. The wounds of the sheep were examined for the presence of calliphorid larvae. The fly larvae associated with myiasis were collected from the infested regions with the help of a brush and forceps and preserved in 90 % ethanol for laboratory studies.</div></div><div><h3>Results</h3><div>During the present study, a total of 6441 sheep were screened for the infestation of calliphorids (blowflies) out of which 220 were found harbouring the fly larvae. The ewes (79.09 %) were found more susceptible than males (20.90 %). The incidence and prevalence of myiasis in sheep showed seasonal specificity, with the highest rate in autumn followed by summer, whereas no case of myiasis was recorded in spring and winter. The larvae causing this condition were identified as 3rd instar larvae of <em>Chrysomya megacephala</em> (Fabricius, 1794) <em>and Lucilia sericata</em> (Meigen, 1826). <em>Chrysomya megacephala</em> (Fabricius) has been documented for the first time in India as the causative agent of myiasis in sheep. Predictable mortality rate among the seasons and selected sites was also investigated.</div></div><div><h3>Conclusion</h3><div>The present work is one of the first kind of studies from Kashmir Himalaya compiling information on myiasis-causing flies in sheep, particularly those in the Calliphoridae, and this study will act as a roadmap for further studies on the issue and its management.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101298"},"PeriodicalIF":1.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trypanosoma cruzi DTU TcI disproportionately associated with fatal T. cruzi myocarditis in dogs in Texas, USA","authors":"Carolyn L. Hodo ,&nbsp;Lisa D. Auckland ,&nbsp;Rachel Curtis-Robles ,&nbsp;Barbara C. Lewis ,&nbsp;Sarah A. Hamer","doi":"10.1016/j.vprsr.2025.101293","DOIUrl":"10.1016/j.vprsr.2025.101293","url":null,"abstract":"<div><div>Acute heart failure and sudden death are possible outcomes of <em>Trypanosoma cruzi</em> infection in domestic dogs. Understanding which discrete typing units (DTUs) are present in fatal canine Chagas disease cases may help explain variation in disease outcome among infected dogs. We conducted a retrospective study of cases from the Texas A&amp;M Veterinary Medical Diagnostic Laboratory and sampled heart tissue from 62 dogs with a histopathologic diagnosis of Chagas disease from 2007 to 2013 for which formalin-fixed paraffin embedded heart tissue was available. Extracted DNA was subjected to qPCRs for <em>T. cruzi</em> and DTU determination. Most dogs (63 %) were less than a year of age at time of death, and males and females were equally represented. Most cases died suddenly with clinical signs of less than one week or experienced sudden death with no prior clinical signs (79 %). Cardiac histopathology features included characteristic inflammation with cardiomyocyte degeneration +/− <em>T. cruzi</em> amastigotes. Lesions in other examined organs included hepatic congestion (37/47), pulmonary edema (24/40), and neuroinflammation (5/11), with <em>T. cruzi</em> amastigotes observed in the brain of one dog. Of 62 dog hearts sampled, DTU was determined in 34, with 33 TcI and one TcIV. Given vectors, wildlife, and dogs living with <em>T. cruzi</em> infections across Texas show both TcI and TcIV infections, this study provides evidence to support an association between TcI and fatal Chagasic heart disease in dogs, which may have prognostic relevance. More work is needed to better understand this association and understand the role of other DTUs circulating in the US.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101293"},"PeriodicalIF":1.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144296825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of fenbendazole in small ruminants on Southern New England farms","authors":"Eleanor Kharasch ,&nbsp;Rachael Gately ,&nbsp;Christopher Odoom ,&nbsp;Owen Gallagher ,&nbsp;Meera Gatlin","doi":"10.1016/j.vprsr.2025.101292","DOIUrl":"10.1016/j.vprsr.2025.101292","url":null,"abstract":"<div><div>Parasite control is a major challenge and contributor to economic loss and animal illness in the small ruminant industry. Given the burden of parasitism and lack of anthelmintic resistance surveillance, the purpose of this study was to determine the clinical efficacy of fenbendazole in sheep and goats in Southern New England in the United States. Thirty sheep and eighty goats from nineteen farms in Connecticut, Massachusetts, and Rhode Island were sampled for fecal egg counts (FEC) pre- and post-administration of a 10 mg/kg oral dose of fenbendazole (Panacur® 10 % oral suspension). This study found an average fecal egg count reduction (FECR) of 41 % (90 % CI [0, 0.45], <em>p</em> &lt; 0.05), with 87.3 % (96/110) of animals failing to meet the minimum clinically recommended threshold of 90 % for FECR. Of the individual animals that exhibited a failure of treatment, 35 % (34/96) had an increased FEC after treatment. Reduced efficacy of fenbendazole against strongyle-type gastrointestinal nematodes (GIN) in small ruminants was observed on all nineteen farms. Farms without animal movement off and back onto the property had a median FECR of 54.2 %, and farms that had movement had a median FECR of 25 %, but this was not statistically significant. Seasonal timing of FEC tests are important for accurate detection and appropriate treatment. A two-sample <em>t</em>-test confirmed that two groups, divided by a date of June 12, have a significantly different average initial FEC. This study emphasizes the unreliability of fenbendazole efficacy in these populations and the necessity of monitoring FECs alongside on-farm management tools.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101292"},"PeriodicalIF":1.4,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of Neospora caninum infection in cattle and yaks in the northern mountainous regions of Kyrgyzstan","authors":"Veli Y. Çirak ,&nbsp;Ihsan Kisadere ,&nbsp;Hüseyin Cihan ,&nbsp;Mambetaly Tursumbetov ,&nbsp;Christian Bauer","doi":"10.1016/j.vprsr.2025.101291","DOIUrl":"10.1016/j.vprsr.2025.101291","url":null,"abstract":"<div><div><em>Neospora caninum</em> is a cyst-forming coccidian parasite, with dogs and a few other canids serving as definitive hosts, and a wide range of intermediate mammalian hosts, including cattle and other bovids. Neosporosis is distributed worldwide and represents a major cause of abortion, stillbirth, and neonatal mortality in cattle. Kyrgyzstan, a mountainous country in Central Asia, has a predominantly small-scale agricultural economy. This study aimed to provide initial data on the occurrence of <em>N. caninum</em> infections in bovids in Kyrgyzstan. Plasma samples from 471 cattle and 27 yaks of both sexes and various ages, collected across ten rural communities in the northern mountainous regions of the country, were tested for <em>N. caninum</em>-specific antibodies using a commercial enzyme-linked immunosorbent assay (IDEXX Neospora X2 Ag Test), following the manufacturer's instructions. Antibodies against <em>N. caninum</em> were detected in 71 cattle samples from nine of the ten communities, yielding an apparent seroprevalence of 15.1 % (95 % confidence interval: 11.9–18.6 %). The proportion of seropositive cattle was lower in communities located at altitudes above 1000 m compared to those located below 1000 m. Cattle in communities within the semi-arid climate zone had lower seroprevalence than those in the humid continental and Mediterranean-like continental climate zones. No significant association was found between seropositivity and the sex or age class of the cattle. One yak sample tested seropositive for <em>N. caninum</em> antibodies.</div><div>This study is the first investigation into the occurrence of <em>N. caninum</em> infection in Kyrgyzstan, demonstrating that the pathogen is present among cattle in the mountainous region. Therefore, neosporosis should be considered a potential cause of reproductive disorders in cattle in the country. Further research is needed to identify risk factors and assess the clinical and economic impact of bovine neosporosis in Kyrgyzstan.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"62 ","pages":"Article 101291"},"PeriodicalIF":1.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization, phylogenetic analysis and histopathological changes of Eimeria species infecting domestic pigeons (Columba livia domestica) in Giza governorate, Egypt","authors":"Fatema A. Gamal ,&nbsp;Azza M. Abdel-Wahab ,&nbsp;Marwa M. Khalifa ,&nbsp;Marwa S. Khattab ,&nbsp;Ahmed M. Nassar","doi":"10.1016/j.vprsr.2025.101290","DOIUrl":"10.1016/j.vprsr.2025.101290","url":null,"abstract":"<div><div>The study was aimed to perform molecular identification of <em>Eimeria</em> spp. infecting domestic pigeons in Giza governorate, Egypt. Also, to study its life cycle in experimentally infected squabs. Molecular identification revealed five species of <em>Eimeria</em> (<em>E. labbeana</em>, <em>E.columbarum</em>, <em>E.columbae</em>, <em>E.columbinae and E. chalcoptereae</em>). Molecular characterization using two primers, the PCR products of <em>Eimeria</em> spp. revealed that the amplified products of the 18S rRNA region had ∼400 bp and 500 bp. Sequencing and phylogenetic analysis of the PCR results indicated that the species under investigation were <em>E. labbeana</em>, <em>E.columbarum</em>, <em>E.columbae</em>, <em>E.columbinae</em> and <em>E. chalcoptereae</em> under accession numbers OR420916, OR554388, OR562036, OR420908 and OR553660, respectively. Experimental infection of squabs with mixed <em>Eimeria</em> spp. revealed that trophozoites, schizonts, macro, microgametocytes and immature oocysts appeared at 12–36 h, 3–4 days, 4–5 days and 6 days post infection (PI), respectively. Histopathological changes in different intestinal parts showed sloughed epithelium, shortened villi and leukocytic cells infiltration. This study is considered as the first molecular characterization and phylogenetic analysis of <em>Eimeria</em> spp. infecting domestic pigeons in Egypt.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"62 ","pages":"Article 101290"},"PeriodicalIF":1.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First identification of zoonotic assemblages A and B of Giardia duodenalis in mules and donkeys in Brazil","authors":"Estela de Aquino Oliveira ,&nbsp;Débora Regina Romualdo da Silva ,&nbsp;Lívia Cavalari Morales ,&nbsp;Camila Michele de Souza Hossotani ,&nbsp;Michel dos Santos Pinto ,&nbsp;João Alfredo Biagi Camargo Neto ,&nbsp;Alex Akira Nakamura ,&nbsp;Katia Denise Saraiva Bresciani","doi":"10.1016/j.vprsr.2025.101289","DOIUrl":"10.1016/j.vprsr.2025.101289","url":null,"abstract":"<div><div>Research on <em>Giardia duodenalis</em> in mules and donkeys is scarce in Brazil, especially in molecular epidemiology. Therefore, we performed molecular detection and genotyping of <em>G. duodenalis</em> in mules and donkeys from São Paulo, Brazil. Through diagnostic techniques, we compared the agreement between the parasitological examination and the Polymerase Chain Reaction (PCR), through diagnostic techniques. A total of 110 animals, 46 mules, and 64 donkeys were investigated in this study. Fecal samples were collected directly from the rectal ampulla of the animals and submitted to the parasitological investigation of <em>Giardia</em> spp. cysts using the Faust technique. An aliquot of the sample was also subjected to DNA extraction and molecular detection of <em>Giardia</em> spp. was performed by nested PCR of the 18S rRNA gene. Positive samples were subjected to amplification of the additional genes β-giardin (βg), Triose Phosphate Isomerase (Tpi), and Glutamate Dehydrogenase (Gdh) for genotyping. In our study, we identified 4.35 % (2/46) and 1.56 % (1/64) of <em>G. duodenalis</em> in mules and donkeys, respectively. Through microscopic examination, we detected the parasite in 0.9 % (1/110) of the combined samples. Amplifying and sequencing the Gdh revealed that two samples presented 100 % genetic similarity with assemblage B (sub-assemblage BIV) and one with assemblage A (sub-assemblage AII). In this research, through molecular characterization, we identified, for the first time in the world, the potentially zoonotic assemblages A and B in mules and, for the first time, assemblage B in donkeys in Brazil.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"62 ","pages":"Article 101289"},"PeriodicalIF":1.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eugenol-parasite interactions in ten spotted live-bearer fish Cnesterodon decemmaculatus: Seasonal and host-related effects","authors":"M.C. Vercellini ,&nbsp;A.C. Carranza-Martín ,&nbsp;L. Morote ,&nbsp;F. Maschi ,&nbsp;M.M. Montes ,&nbsp;M.A. Ayala","doi":"10.1016/j.vprsr.2025.101288","DOIUrl":"10.1016/j.vprsr.2025.101288","url":null,"abstract":"<div><div>The widespread use of synthetic antibiotics and chemicals in aquaculture to control parasitic infections poses environmental and health risks, including antibiotic resistance and harm to aquatic organisms. Consequently, there is growing interest in natural, sustainable alternatives for parasite control in both wild fish and aquaculture. This study examines the efficacy of eugenol and its effects on parasitic infections and infestations in the ten spotted live-bearer fish <em>Cnesterodon decemmaculatus</em>, found in South American freshwater ecosystems. The study focuses on ectoparasites such as <em>Trichodina</em> spp., as well as endoparasites including Echinostomatidae, and <em>Ascocotyle</em> sp., which affect the skin and gills, <em>Ascocotyle</em> sp. metacercariae in the heart, <em>Pygidiopsis</em> sp. metacercariae infecting the liver and coelomic cavity, and <em>Saccocoelioides kirchnerii</em> infecting the intestine. Seasonal and host sex-related differences in parasitic mean abundance (MA) and prevalence were also considered. Seasonal variations were observed in Echinostomatidae, <em>Trichodina</em> spp., and <em>Ascocotyle</em> sp. Eugenol significantly reduced the MA of <em>Trichodina</em> spp. (<em>P</em> = 0.001). Larger fish exhibited higher parasitic MA, with significant correlations observed for total length (TL) (<em>P</em> = 0.03) and standard length (SL) (<em>P</em> = 0.02). This study contributes to the understanding of natural antiparasitic agents in aquaculture and their potential for sustainable parasite management.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"62 ","pages":"Article 101288"},"PeriodicalIF":1.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epizootiology and pathophysiology of bovine oriental theileriosis in Mizoram, India","authors":"Champak Deka ,&nbsp;Kalyan Sarma ,&nbsp;Chethan Gollahalli Eregowda ,&nbsp;Parimal Roychoudhury ,&nbsp;Neeraj Thakur ,&nbsp;Dilip Nama ,&nbsp;Kiran Jayappa ,&nbsp;Satyabrat Dutta ,&nbsp;Dhruba Das ,&nbsp;Palash Jyoti Sonowal ,&nbsp;Rahul Singh Arya ,&nbsp;Thingujam Chaa Tolenkhomba ,&nbsp;Dibyajyoti Talukdar ,&nbsp;Priyanka Choudhary ,&nbsp;Girin Kalita","doi":"10.1016/j.vprsr.2025.101287","DOIUrl":"10.1016/j.vprsr.2025.101287","url":null,"abstract":"<div><div>Bovine oriental theileriosis is an important tick-borne haemoprotozoan disease caused by <em>Theileria orientalis</em>. The present study was carried out to investigate the prevalence, associated risk factors, and haemato-biochemical and pathological alterations associated with naturally occurring <em>T. orientalis</em> infection in cattle, an emerging disease in Mizoram, India. A polymerase chain reaction (PCR) detection based cross-sectional study on the prevalence of <em>T. orientalis</em> infection in cattle was undertaken from June 2019 to January 2022. Blood samples were collected from the clinically infected crossbred cattle (<em>n</em> = 12) to examine the haemogram, and serum samples were used for evaluating biochemical profile and oxidant-antioxidant status. The diagnosis of <em>T. orientalis</em> infection in cattle was carried by microscopic blood smear examination and further confirmed by PCR. Haemogram and serum biochemical profile were evaluated by automated blood cell counter and clinical chemistry analyzer, respectively. Oxidant and antioxidant parameters were estimated by using commercially available assay kits. In the present study, PCR was found to be more sensitive than microscopic blood smear examination, and an overall prevalence of 7.29 % was recorded for <em>T. orientalis</em> infection by PCR assay. Tick infestation, uncemented floor, and absence of acaricidal treatment were found be potential risk factors for <em>T. orientalis</em> infection. Haemogram and serum biochemical findings revealed anaemia, thrombocytopenia, hypoproteinemia, elevated aspartate aminotransferase (AST) and blirubin levels, and azotemia. The mean values of superoxide dismutase (SOD), glutathione (GSH) and total antioxidant capacity (TAC) were significantly (<em>P</em> &lt; 0.05) lower, whereas the mean value lipid hydroperoxide (LPO) were significantly (<em>P</em> &lt; 0.05) higher in cattle infected with <em>T. orientalis</em>, suggesting oxidative stress. When total erythrocyte count (TEC) was correlated with LPO (<em>r</em> = −0.781, <em>P</em> &lt; 0.01), it revealed a negative correlation, while in case of GSH (<em>r</em> = 0.744, <em>P</em> &lt; 0.01), SOD (<em>r</em> = 0.794, <em>P</em> &lt; 0.01), and TAC (<em>r</em> = 0.796, <em>P</em> &lt; 0.01), a positive correlation was noticed. The correlation of oxidative stress indices with TEC suggested that erythrocyte peroxidation could play an important role in the pathogenesis of anaemia induced by <em>T. orientalis</em> infection. There are very few studies on the pathological aspects of <em>T. orientalis</em> infection in cattle. Thus, the outcome of the present study could add valuable information to the current existing knowledge about the disease.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"62 ","pages":"Article 101287"},"PeriodicalIF":1.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Toxoplasma gondii in green turtles (Chelonia mydas): Implications for wildlife and ecosystem health","authors":"Alinne Petris ,&nbsp;Juliane Araújo Greinert-Goulart ,&nbsp;Paula Angélica Roratto ,&nbsp;Brenda Letícia Tiedt ,&nbsp;Tiffany Christiny Emmerich da Silva ,&nbsp;Daniela Fink ,&nbsp;Diego Averaldo Guiguet Leal","doi":"10.1016/j.vprsr.2025.101286","DOIUrl":"10.1016/j.vprsr.2025.101286","url":null,"abstract":"<div><div><em>Toxoplasma gondii</em> is a protozoan parasite with varied implications for human and animal health. However, knowledge about its ecoepidemiology and the infection of a wide range of marine animals are incipient or poorly explored in many coastal areas worldwide. The goal of this study was to investigate the presence of <em>T. gondii</em> in green turtles (<em>Chelonia mydas</em>) found along the northern coast of Santa Catarina State, Brazil. A variety of tissues from eight green turtles were tested using Nested-PCR and <em>T. gondii</em> DNA was detected in brain and liver of two green turtles. This study provides the first evidence of <em>T. gondii</em> in marine turtles highlighting the importance of considering this species as accidental hosts for the parasite.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"62 ","pages":"Article 101286"},"PeriodicalIF":1.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}