Veterinary parasitology, regional studies and reports最新文献

{"title":"Anti-Toxoplasma gondii and Leishmania spp. antibodies in household and shelter dogs in the municipality of Arapiraca, Alagoas, Northeastern Brazil","authors":"Carolina Ferreira de Oliveira ,&nbsp;Pedro Levi Nascimento Oliveira ,&nbsp;Jussara Nayanne dos Santos Nascimento ,&nbsp;Fagner D'ambroso Fernandes ,&nbsp;Davi Dayan Assenheimer ,&nbsp;Fernanda Silveira Flores Vogel ,&nbsp;Jonatas Campos de Almeida","doi":"10.1016/j.vprsr.2025.101310","DOIUrl":"10.1016/j.vprsr.2025.101310","url":null,"abstract":"<div><div>Domestic dogs are considered effective sentinels for zoonotic diseases due to their abundance, ecological ubiquity, and frequent exposure to pathogens through scavenging and carnivorous behaviors. This study aimed to determine the seroprevalence of antibodies against <em>Toxoplasma gondii</em> and <em>Leishmania</em> spp. and to investigate potential infection risk factors in household and shelter dogs in Arapiraca, a semi-arid municipality in Northeastern Brazil. A total of 292 serum samples were analyzed, comprising 142 from shelter dogs and 150 from household dogs. The Indirect Immunofluorescence Assay (IFA) was used for serological testing, with cut-off titers of ≥1:16 for <em>T. gondii</em> and ≥ 1:40 for <em>Leishmania</em> spp. Antibodies against <em>T. gondii</em> were detected in 5.8 % (17/292) of the dogs, with a higher frequency in shelter dogs 8.4 % (12/142) compared to household dogs at 3.3 % (5/150). For <em>Leishmania</em> spp., 9.2 % (27/292) of the animals tested seropositive: 11.2 % (16/142) in shelter dogs and 7.3 % in household dogs (11/150). None of the seropositive animals presented clinical signs of disease. Statistical analysis did not reveal significant associations between seropositivity and the evaluated variables. These findings highlight the role of dogs as silent reservoirs and sentinels for zoonotic pathogens, reinforcing the need for active surveillance strategies, particularly in socioeconomically vulnerable and ecologically sensitive areas. The results also suggest that canine serological monitoring may serve as a useful indicator of environmental risk for human exposure to toxoplasmosis and leishmaniasis.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101310"},"PeriodicalIF":1.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Albendazole against gastrointestinal nematodes of pigs in the sub-humid savanna zone of southeastern Nigeria","authors":"I.K. Idika,&nbsp;C.F. Obi,&nbsp;T.A. Nzeakor,&nbsp;G.E. Aneru,&nbsp;S.I. Aideyan,&nbsp;M.I. Okpala,&nbsp;C.O. Nwosu","doi":"10.1016/j.vprsr.2025.101303","DOIUrl":"10.1016/j.vprsr.2025.101303","url":null,"abstract":"<div><div>Albendazole is the most widely used anthelmintic in the Nigerian pig industry; however, its efficacy is increasingly compromised by emerging resistant strains of gastrointestinal nematodes (GINs). This study evaluated albendazole efficacy in pigs reared in the sub-humid savanna zone of southeastern Nigeria using a faecal egg count reduction test (FECRT) to assess anthelmintic resistance. Faecal samples were collected from 130 randomly selected pigs across 13 farms and screened for GIN infections. Six farms, with no anthelmintic treatment for at least three months, were selected for FECRT; ten infected pigs per farm were randomly chosen, with pre-treatment faecal egg counts (FEC) assessed prior to albendazole administration and post-treatment FEC recorded on day 10. Overall, GIN prevalence was 63.1 %, with mixed infections representing 74.6 % of cases. Results indicated resistance among GINs, particularly strongyles and trichurids. Albendazole-resistant GINs were detected in three farms, while two farms exhibited susceptibility and one yielded an inconclusive result. Specifically, albendazole-resistant strongyles were observed in one farm, with susceptible populations in four farms and inconclusive findings in one. Resistant trichurids were found in two farms, whereas ascarids remained susceptible across all farms. These findings demonstrate variable albendazole efficacy against GINs in pigs, with significant resistance among trichurids and strongyles. The detection of albendazole-resistant GIN strains in the sub-humid savanna zone of southeastern Nigeria underscores the need for regular monitoring and integrated parasite management strategies to mitigate the development of resistance.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101303"},"PeriodicalIF":1.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic loss monitoring due to cystic echinococcosis (CE) infection in livestock in major cities of Punjab Pakistan","authors":"Maryam Aftab ,&nbsp;Naseer Ali Shah ,&nbsp;Haroon Ahmed ,&nbsp;Muhammad Kamran","doi":"10.1016/j.vprsr.2025.101308","DOIUrl":"10.1016/j.vprsr.2025.101308","url":null,"abstract":"<div><div>Cystic echinococcosis (CE) is a parasitic disease caused by the larval stage of the tapeworm <em>Echinococcus granulosus</em>. The objective of the current study was to determine the prevalence of animals (buffaloes and cows) infected by CE in the major cities of Punjab and the economic loss to livestock traders due to the condemnation of infected vital organs, including the liver and lungs, which are used as food for humans. It is a zoonotic disease affecting both animals and humans (intermediate hosts). This study was conducted over a period of six months, from October 2021 to March 2022. The animals were examined for CE infection at the Sihala slaughterhouse in Rawalpindi, which is a hub for livestock across Punjab. A total of 1195 animals, including 1036 buffaloes and 159 cows, were examined for CE. Postmortem, 255 animals were found to be infected with CE. The prevalence of CE infection was 18.07 % and 3.26 % in buffaloes and cows, respectively. The lung was the most commonly infected organ (54.51 %), followed by the liver (45.49 %). CE has a significant economic impact on the livestock industry owing to its effects on animal morbidity and mortality. The economic losses caused by infected livers were calculated to be 1589.2 USD, and for infected lungs, it was estimated to be 303.02 USD for the year 2022. Destruction of vital organs in animals has serious consequences. Therefore, investigating the prevalence of CE among livestock in highly affected areas can help to control the transmission of the parasite in intermediate hosts. In addition, it is crucial to develop effective control strategies to prevent significant economic losses.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101308"},"PeriodicalIF":1.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of an Ehrlichia genospecies related to agents of human monocytic ehrlichiosis in Ixodes ticks from northern Chile","authors":"Adriana Santodomingo ,&nbsp;Richard Thomas ,&nbsp;Catalina Parragué-Migone ,&nbsp;Sebastián Muñoz-Leal ,&nbsp;Gerardo Acosta-Jamett","doi":"10.1016/j.vprsr.2025.101309","DOIUrl":"10.1016/j.vprsr.2025.101309","url":null,"abstract":"<div><div>The family <em>Anaplasmataceae</em> comprises obligate intracellular bacteria, some of which are tick-borne pathogens of medical and veterinary concern. In South America, limited research on <em>Anaplasmataceae</em> restricts our understanding of their epidemiology, diversity, and host-vector dynamics. We conducted a molecular survey of <em>Anaplasmataceae</em> on <em>Ixodes</em> spp. ticks collected from rodents in northern Chile. Ticks were morphologically identified and screened by PCR targeting the 16S rRNA (<em>rrs</em>), <em>gltA</em>, and <em>groEL</em> loci. Genetic analyses revealed an <em>Ehrlichia</em> genospecies related to the <em>Ehrlichia chaffeensis</em>-associated (ECA) clade, detected in an <em>Ixodes</em> nymph parasitizing a <em>Phyllotis darwini</em> rodent. The ECA clade includes zoonotic species such as <em>E. chaffeensis</em> and <em>Ehrlichia muris</em> subsp. <em>euclarensis</em>. This is the first evidence of an ECA-related <em>Ehrlichia</em> genospecies associated with the Neotropical <em>Ixodes sigelos</em> tick complex. Although its pathogenicity is unknown, its phylogenetic link to zoonotic species warrants further investigation. Additional surveillance of <em>Ixodes</em> spp. and wild mammals is essential to clarify its distribution, ecological role, and public health implications.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101309"},"PeriodicalIF":1.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first morphological and molecular characterization of wing louse (Lipeurus caponis) from ornamental and indigenous chickens of West-Coast India","authors":"Shirish Dadarao Narnaware,&nbsp;Nibedita Nayak,&nbsp;Prasastha Vemula,&nbsp;Susitha Rajkumar,&nbsp;Amiya Ranjan Sahu","doi":"10.1016/j.vprsr.2025.101307","DOIUrl":"10.1016/j.vprsr.2025.101307","url":null,"abstract":"<div><div>Lice infestation in poultry presents a substantial challenge to poultry farmers, with adverse implications for bird health, welfare, and productivity. In this study, the outbreak of wing louse infestation was recorded in ornamental and improved indigenous chicken varieties of an organized poultry farm located in the west-coast of India during the winter season. The lice were found to be attached to the underside of the wings between the feather barbs on the primary or secondary wing feathers. In addition to the active lice population, white clumps of lice's eggs (nits) were found attached at the base of feathers in the breast and thigh region. The affected birds showed signs of feather loss, irritation, itching, annoyance, restlessness, scratching, and feather plucking. Morphologically, the adult lice were dark brown colored, wingless, and elongated with dorso-ventrally flattened bodies. The female lice were significantly larger than the males in body length, post-antennal head width, thorax width, abdominal width, and abdominal length. The body structure resembled <em>Lipeurus caponis</em> when observed under a stereo microscope. For molecular characterization, the total genomic DNA extracted from whole lice was used to amplify the mitochondrial COI (Cytochrome Oxidase Subunit I) gene using universal primers. The PCR product obtained was sequenced, and the gene sequence was submitted to GenBank. Based on the phylogenetic analysis of the COI gene, of the present study's sequences were clustered with <em>L. caponis</em> hosted by Indian peacocks of China and Jungle fowl of the UK. This is the first record of the COI sequence of <em>L. caponis</em> from India.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101307"},"PeriodicalIF":1.4,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceptions and interventions concerning coccidiosis in Dutch sheep farms","authors":"Mark van der Heijden ,&nbsp;Snorre Stuen ,&nbsp;Inge Santman-Berends ,&nbsp;Carlijn ter Bogt-Kappert ,&nbsp;Caroline Verduijn ,&nbsp;Piet Vellema","doi":"10.1016/j.vprsr.2025.101306","DOIUrl":"10.1016/j.vprsr.2025.101306","url":null,"abstract":"<div><div>Coccidiosis in sheep is a protozoan disease of young lambs that is not yet fully understood, and its control still largely relies on the use of coccidiostats, despite development of resistance. It is hypothesized that in the Netherlands a proper diagnosis is not always made before treatment is initiated, possibly leading to an overuse of coccidiostats. The aims of this study are to investigate the perceptions and interventions of Dutch sheep farmers and their veterinarians regarding coccidiosis, with special attention to treatment and the use of anticoccidial drugs. An online survey was completed by 114 sheep farmers and 57 veterinary practitioners. The surveyed sheep farmers appeared to be genuinely interested in coccidiosis, with three-quarters considering it an important problem in their flock. Seventy per cent of the sheep farmers and 84 % of the veterinarians reported diarrhoea as the most important symptom of coccidiosis. Almost three-quarters of sheep farmers conducted faecal examination during the two preceding years, 87 % per cent have performed treatments, and half of them used coccidiostats annually. There was a wide variety of treatment approaches. Standard treatment of lambs without any clinical indication, or before 4 weeks of age, and treatment of adult sheep were reported by 26 %, 13 %, and 13 % of responding farmers, respectively, and the latter two are considered as incorrect treatments. In contrast, veterinarians estimated that, on average, coccidiostats are prescribed on 30 % of sheep farms in their practice, with treatment primarily based on clinical signs and faecal examination results. Potential obstacles to a correct diagnosis included practical difficulties in collecting faecal samples, and additional costs. Remarkably, questionable treatments were common, including treatments without a history of coccidiosis, treatments of lambs younger than one month of age and treatments of adult sheep. Although correct dosage of coccidiostats is crucial, 43 % of veterinarians advised dosing based on the heaviest animals, while in most cases weight was visually estimated by both farmer and veterinarian. Unfortunately, treatments were frequently applied without a proper diagnosis, and the applied dosage was often not correct, which may lead to unnecessary and incorrect use of coccidiostats resulting in an increased risk of resistance development. Since veterinarians are regarded as the most important advisors, they play a key role in the solution. By supporting veterinarians, preventive measures and more prudent use of coccidiostats can be promoted.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101306"},"PeriodicalIF":1.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution to an efficient diagnosis of Fasciola hepatica infection in naturally infected cattle","authors":"Natã Medeiros Godinho ,&nbsp;Camila Zomer Spíndola ,&nbsp;Márcia Sangaletti Lavina ,&nbsp;Andreas Lazaros Chryssafidis ,&nbsp;Anderson Barbosa de Moura","doi":"10.1016/j.vprsr.2025.101301","DOIUrl":"10.1016/j.vprsr.2025.101301","url":null,"abstract":"<div><div>The control of fascioliasis caused by <em>Fasciola hepatica</em> depends on early detection and assessment of infection severity. A definitive diagnosis is the post-mortem recovery of flukes in the hepatic ducts and gallbladder, which is not feasible for herd monitoring. Coproparasitological techniques based on egg detection in feces are commonly used. This study evaluated the accuracy of the Dennis, Stone, and Swanson (DSS), quatro tamises (QT), and Gordon and Whitlock modified (GWM) techniques using parasitized livers as the gold standard. Of 784 carcasses, 105 (13.39 %) were parasitized by <em>F. hepatica</em>. The sensitivity was 43.8 % for DSS, 86.6 % for QT, and 7.6 % for GWM. In comparison with the gold standard, agreement on the Kappa scale was weak for GWM (0.10), moderate for DSS (0.45), and excellent for QT (0.84). Additionally, when comparing the techniques with each other, modest agreement was observed between QT and GWM (0.20), and moderate agreement between DSS and GWM (0.57) and DSS and QT (0.48). Parasite intensity did not influence (<em>P</em> &gt; 0.05) the sensitivity of DSS and QT. These findings indicate that QT is the most sensitive and reliable coproparasitological technique for diagnosing <em>F. hepatica</em> infection in naturally infected cattle.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101301"},"PeriodicalIF":1.4,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, geospatial distribution, and risk factors for bovine cysticercosis across diverse states of Brazil","authors":"Fabio Martins Guerra Nunes Dias ,&nbsp;Laura Lúcia dos Santos Oliveira ,&nbsp;Fredson Vieira e Silva ,&nbsp;André Guimarães Maciel e Silva ,&nbsp;José Bento Sterman Ferraz ,&nbsp;Guilherme Jordão de Magalhães Rosa","doi":"10.1016/j.vprsr.2025.101305","DOIUrl":"10.1016/j.vprsr.2025.101305","url":null,"abstract":"<div><div>This study examined the prevalence and spatial distribution of bovine cysticercosis in Brazil between 2018 and 2020, focusing on its associations with factors such as the human development index (HDI), population density, cattle weight, age, and sex. The analysis included data from approximately 22 million bovine carcasses from 10 of Brazil's 26 states, revealing an overall carcass prevalence of 0.09 %. Although this prevalence is considered low, it varies significantly across different states and microregions. The geospatial study of bovine cysticercosis identified clusters with high prevalence (high-high) and outliers (high-low) in Brazilian microregions. The study revealed significant associations between HDI, population density, and cattle age and the prevalence of cysticercosis; however, the models lacked robustness. In Brazilian microregions, these findings emphasize the need for targeted interventions and intensified surveillance in higher-risk areas, reinforcing regional variations and supporting the development of more effective public policies and health interventions.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101305"},"PeriodicalIF":1.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importation of the exotic tick Amblyomma geoemydae into Sweden via illegally introduced turtles (Geoemyda spengleri) from Thailand","authors":"Giulio Grandi ,&nbsp;Anna Omazic ,&nbsp;Cesar Guevara ,&nbsp;Anton de Jong ,&nbsp;Lidia Chitimia-Dobler","doi":"10.1016/j.vprsr.2025.101304","DOIUrl":"10.1016/j.vprsr.2025.101304","url":null,"abstract":"<div><div>Illegal importation of animals as pets might be followed by the introduction of exotic tick species, potential vectors of pathogens that can have an impact on wildlife and/or domestic animals and on humans. Therefore, permanent measures of surveillance and control on imported reptiles are emphasized. In February 2024, black-breasted leaf turtles (<em>Geoemyda spengleri</em>) (<em>n</em> = 31) were illegally imported into Sweden from Thailand. The turtles were sent by the Swedish Customs Criminal Department West in Gothenburg to a public aquarium and terrarium (Tropicarium, Kolmården, Östergötland county) to be kept in quarantine. At the Tropicarium the turtles were found to be infested by ticks. All ticks (<em>n</em> = 3) found were removed and stored in ethanol. The finding (including photos of the ticks) was reported to the Swedish Veterinary Agency (SVA, Uppsala) using the Report Tick Tool, a surveillance system developed by the SVA itself. The three collected ticks were identified based on morphology and genetics as <em>Amblyomma geoemydae</em> nymphs. This discovery demonstrates that SVA's surveillance tool can function as an early warning system for newly introduced tick species and to promptly identify exotic tick species which can carry viruses, bacteria, and/or parasites that are not currently present in the country.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101304"},"PeriodicalIF":1.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular evidence of Aelurostrongylus abstrusus in domestic cats from Africa and a synopsis of its occurrence across the continent","authors":"Ioana Bianca Mitrea ,&nbsp;Petr Cibulka ,&nbsp;Angela Monica Ionică ,&nbsp;Nonzuzo Audrey Ndlovu ,&nbsp;Norman Leo Mukarati ,&nbsp;Thokozani Hove ,&nbsp;Noureddine Mechouk ,&nbsp;Mihai Sorin Cernea ,&nbsp;Andrei Daniel Mihalca","doi":"10.1016/j.vprsr.2025.101302","DOIUrl":"10.1016/j.vprsr.2025.101302","url":null,"abstract":"<div><div>Domestic cats (<em>Felis catus</em>) can be infected with a variety of cardio-pulmonary nematodes. Although <em>Aelurostrongylus abstrusus</em> is widely distributed globally, reports from Africa are scarce and refer mainly to wild felids. Moreover, some of these reports are considered as misidentifications. The only report in domestic cats in Africa is more than 20 years old and originated from Kenya. The aim of this study was to evaluate the presence of <em>A. abstrusus</em> in domestic cats from Harare, Zimbabwe morphologically on light microscopy and confirmation by molecular means.</div><div>A total of 57 domestic cats were included in this study, and fecal samples were analyzed using the Baermann technique. The detected larvae were collected, morphologically identified, and subsequently confirmed through molecular analysis by targeting three genes.</div><div>One sample out of 57 tested positive for first-stage larvae (L1) and was identified as <em>Aelurostrongylus abstrusus</em> based on morphological characteristics. Molecular analysis subsequently confirmed the species identity.</div><div>The present study is the first molecular confirmation of <em>Aelurostrongylus abstrusus</em> in domestic cats from Africa and the first case in domestic cats from southern Africa. Future studies are recommended to further investigate its distribution and epidemiology.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101302"},"PeriodicalIF":1.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}