Veterinary parasitology, regional studies and reports最新文献

{"title":"Comparative susceptibility of native chicken breeds of Bangladesh to experimental Eimeria tenella infection","authors":"Dipesh Aryal,&nbsp;Kazi Farah Tasfia,&nbsp;Al Nur Tarak,&nbsp;Asmita Bhujel,&nbsp;Md. Shahiduzzaman","doi":"10.1016/j.vprsr.2025.101353","DOIUrl":"10.1016/j.vprsr.2025.101353","url":null,"abstract":"<div><div><em>Eimeria tenella</em> is the major protozoan parasite that causes coccidiosis in chickens, and its clinical and pathological response shows significant variations among breeds. This disease has a major economic impact on growers and the poultry industry worldwide. Very little information exists concerning the challenge infections of native chicken breeds of Bangladesh. The present study was undertaken to investigate and compare the pathology and clinical manifestations of experimental <em>E. tenella</em> infection among native (indigenous) chicken breeds of Bangladesh (Hilly, Naked Neck, and Deshi), in comparison to the susceptible exotic Sonali breed. In order to achieve this, a controlled experimental infection was carried out, and weight changes, Feed Conversion ratio (FCR), OPG counts, lesion severity, qPCR quantification of oocysts output, and dropping score were measured at 6, 10, and 14-Days post-infection (DPI). Distinct breed-specific differences in susceptibility were elicited. Sonali chickens exhibited the highest oocyst counts, reaching up to 103,200 at 14 DPI, along with significant weight loss and severe cecal pathology marked by diffuse hemorrhagic lesions. On the other hand, the indigenous breeds had low OPG counts as low as 100 in Hilly chickens, insignificant changes in weights, and mild pathological features characterized by reduced inflammation and hemorrhagic enteritis. This study highlights significant breed-specific differences in susceptibility to <em>E. tenella</em>, offering valuable insights into potential resistance or tolerance mechanisms. These findings support the use of native breeds in selective breeding programs aimed at improving disease resistance and underscore the importance of targeted control strategies for effective coccidiosis prevention and management.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101353"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and risk factors for Acanthocheilonema reconditum infection in dogs in the municipality of Lages, Santa Catarina, Brazil","authors":"Faiane Reila Sousa Centenaro Duarte ,&nbsp;Geanice Ledo ,&nbsp;Felipe Rieth de Lima ,&nbsp;Maria Laura Correa Nunes ,&nbsp;Julia Milena Sandri Pereira ,&nbsp;Hanna Christna Antonia Brasil ,&nbsp;Ana Paula Remor Sebolt ,&nbsp;Larissa Américo ,&nbsp;Lúcia Padilha Cury Thomaz de Aquino ,&nbsp;Anderson Barbosa de Moura ,&nbsp;Andreas Lazaros Chryssafidis","doi":"10.1016/j.vprsr.2025.101352","DOIUrl":"10.1016/j.vprsr.2025.101352","url":null,"abstract":"<div><div><em>Acanthocheilonema reconditum</em> (syn. <em>Dipetalonema reconditum</em>) is a filarial nematode transmitted by arthropods (fleas, lice, and ticks), primarily parasitizing the subcutaneous tissue of canids, but also found in perirenal fat. The clinical relevance of <em>A. reconditum</em> is often overshadowed by <em>Dirofilaria immitis</em>, a more pathogenic nematode with which it can be easily confused during diagnosis There are no reports of <em>D. immitis</em> in Lages, Santa Catarina (SC), neither in the Santa Catarina Plateau, Brazil. However, local laboratories observed microfilariae in canine blood samples and requested identification by the Veterinary Parasitology Laboratory of the State University of Santa Catarina. Morphological evaluation confirmed <em>A. reconditum</em> larvae. This study aimed to determine the prevalence of <em>A. reconditum</em> infection and assess risk factors in dogs from Lages. A total of 444 blood samples were collected from dogs in different neighborhoods, with simultaneous coat inspection for ectoparasites. Information on age, sex, origin, breed, ectoparasite control, and presence or absence of clinical signs was obtained through an epidemiological questionnaire to assess risk factors for <em>A. reconditum</em> infection. Blood samples were analyzed by blood smear, modified Knott's test, and immunochromatographic test. The prevalence of <em>A. reconditum</em> was 22.07 % (98/444) by the modified Knott's test, whereas the blood smear detected only 4.27 % (19/444). None of the evaluated samples showed characteristics compatible with <em>D. immitis</em> in the Knott's test, and all tested negative in the immunochromatographic test, indicating the absence of circulating antigen of this species. Ectoparasites were found in 35.58 % (158/444) of the animals, with no general association as a risk factor for <em>A. reconditum</em> infection. Among ectoparasites, Siphonaptera predominated, present in 93.02 % of parasitized animals, with a significant association between <em>Pulex irritans</em> presence (<em>p =</em> 0.003) and <em>A. reconditum</em> infection. This study confirmed the absence of <em>D. immitis</em> in Lages and highlighted a high prevalence of <em>A. reconditum</em>, underscoring the zoonotic potential and the need for further investigations.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101352"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro acaricidal effects of essential oils against Rhipicephalus microplus via bioinformatics targeting of triosephosphate isomerase","authors":"Paola Cristina Colombo ,&nbsp;Arthur Gasetta Batista ,&nbsp;João Guilherme Martins ,&nbsp;Gabriel Nunes de Oliveira ,&nbsp;Marco Tulio da Silva Oliveira ,&nbsp;Giulia Poncione de Araujo ,&nbsp;Sabrina Nathalia Louzada Nogueira ,&nbsp;Rafael Paranhos de Mendonça ,&nbsp;Ricardo Andrade Furtado ,&nbsp;Isabela Cristina Gomes Honório ,&nbsp;Silvio de Almeida-Junior","doi":"10.1016/j.vprsr.2025.101354","DOIUrl":"10.1016/j.vprsr.2025.101354","url":null,"abstract":"<div><div>The tick <em>Rhipicephalus (Boophilus) microplus</em> poses a significant threat to animal health in tropical and subtropical regions, causing substantial economic losses in the meat and dairy industries. This study evaluated the potential of 20 essential oils as acaricidal agents, combining <em>in vitro</em> assays and <em>in silico</em> analyses to predict toxicity and efficacy. In the <em>in vitro</em> tests, the oils were assessed for their ability to reduce tick oviposition. The best results were observed at a 6 % concentration, with <em>Eucalyptus citriodora</em> showing the highest reduction (96.5 %), followed by <em>Betula lenta</em> (88.9 %), <em>Amyris balsamifera</em> (71.3 %), and <em>Cinnamomum cassia</em> (47.1 %). At a 3 % concentration, <em>Amyris balsamifera</em> (67.6 %) and <em>Betula lenta</em> (4.6 %) also showed significant efficacy, while the 1 % concentration had a limited impact on reducing oviposition, not meeting the legal efficacy criterion (&gt;95 %). Concurrently, <em>in silico</em> analyses were conducted using the SwissDock platform to predict the molecular interactions of natural compounds with specific biological targets. The results revealed the free binding energy (estimated ΔG) and the amino acid residues involved in the interactions, allowing for the comparison of the compounds' affinity with reference drugs. The data indicated that some compounds present in the essential oils demonstrated considerable target affinity, suggesting a promising mechanism of action against the tick's reproductive cycle. These findings highlight the potential of essential oils as natural alternatives for controlling <em>Rhipicephalus (Boophilus) microplus</em>, especially at higher concentrations. However, the efficacy at lower concentrations and the toxicity in host animals need further investigation before practical application.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101354"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of BoLA-DRB3 and its association with Anaplasma marginale and Babesia spp. infections in creole cattle of northeastern Colombia","authors":"Jeiczon Jaimes-Dueñez ,&nbsp;Laura Marin-Cossio ,&nbsp;Agustín Gongora-Orjuela ,&nbsp;Ian Murcia-Cueto ,&nbsp;Angela Jimenez-Leaño ,&nbsp;Olivia Marcuzzi ,&nbsp;Guillermo Giovambattista","doi":"10.1016/j.vprsr.2025.101355","DOIUrl":"10.1016/j.vprsr.2025.101355","url":null,"abstract":"<div><div>The <em>BoLA-DRB3</em> gene is one of the most polymorphic loci in cattle, with alleles associated with susceptibility or resistance to several infectious diseases, including leukosis, mastitis, and hemotropic infections. In this study, we assessed the genetic diversity of the <em>BoLA-DRB3</em> gene in three Colombian Creole cattle breeds and examined its association with natural infections by <em>Anaplasma marginale</em> and <em>Babesia</em> spp. in northeastern Colombia. A total of 97 animals (Chino – CrChi, <em>n</em> = 34; Casanareño - CrCAS-, <em>n</em> = 32; Sanmartinero - CrSM-, <em>n</em> = 31) from Arauca, Casanare, Meta, and Santander departments were genotyped using PCR–direct sequencing of the second exon of the <em>BoLA-DRB3</em> gene. Overall genetic diversity was moderate, with a nucleotide diversity of π = 0.086, a mean pairwise distance of 18.97, and 62 segregating sites. Among the breeds, CrCAS showed the highest diversity, followed by CrChi and CrSM. We identified 35 <em>BoLA-DRB3</em> alleles, 34 of which were previously reported in the IPD-MHC database, while one was novel. The CrChi population showed significant deviation from Hardy–Weinberg equilibrium. Two alleles, <em>BoLA-DRB3</em>*001:01 and <em>BoLA-DRB3</em>*025:01:01, were significantly associated with reduced risk of <em>B. bigemina</em> infection in CrSM, whereas <em>BoLA-DRB3*048:02</em> was linked to increased susceptibility to <em>B. bovis</em> in CrChi. This study confirms the high genetic variability of Colombian Creole cattle breeds. The high frequency of alleles associated with <em>B. bigemina</em> infection in CrSM highlights its potential as a genetic reservoir for future studies evaluating the heritability and functionality of these alleles. In contrast, the elevated frequency of a susceptibility-associated allele for <em>B. bovis</em> infection in CrChi, together with evidence of inbreeding, underscores the urgent need for targeted conservation and breeding strategies to preserve genetic diversity and reduce disease vulnerability to infectious diseases in this breed.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101355"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ixodes chilensis Kohls, 1956 (Acari: Ixodida: Ixodidae) in Argentina","authors":"Gabriel L. Cicuttin ,&nbsp;Patrick S. Sebastian ,&nbsp;María Alejandra Lombardo ,&nbsp;Marcela Simone ,&nbsp;María Nazarena De Salvo ,&nbsp;Santiago Nava","doi":"10.1016/j.vprsr.2025.101360","DOIUrl":"10.1016/j.vprsr.2025.101360","url":null,"abstract":"<div><div>The aim of this note is to report the first record of <em>Ixodes chilensis</em> Kohl, 1956 in Argentina. In June 2023, ticks were collected on bat belonging to the genus <em>Histiotus</em> from Dina Huapí, Rio Negro Province, Patagonian region, Argentina. Based on morphological characteristics and analysis of an obtained partial 16S rDNA gene sequence, these ticks (five nymphs) were determined as <em>I. chilensis</em>. This result represents the first record of this tick species outside Chile and expands the knowledge of its geographical distribution in the southern cone of South America.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101360"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visceral leishmaniosis in dogs from western Iran","authors":"Alireza Sazmand ,&nbsp;Georgiana Deak ,&nbsp;Ali Kalantari-Hesari ,&nbsp;Pouria Rafiei ,&nbsp;Navid Etemadi ,&nbsp;Hesam Hajilou ,&nbsp;Mina Malekmohammadi ,&nbsp;Leili Moradi ,&nbsp;Monireh Khordadmehr ,&nbsp;Seyedmousa Motavallihaghi ,&nbsp;Ali Fattahi-Bafghi ,&nbsp;Mariaelisa Carbonara ,&nbsp;Stephanie Oren ,&nbsp;Domenico Otranto","doi":"10.1016/j.vprsr.2025.101351","DOIUrl":"10.1016/j.vprsr.2025.101351","url":null,"abstract":"<div><div><em>Leishmania infantum</em> is a sand fly-borne parasite, causing visceral and mucocutaneous leishmaniosis. Dogs are the main reservoir hosts and play a crucial role in the epidemiology of the infection. This study reports for the first time the presence of <em>L. infantum</em> in sick dogs from a western province of Iran, previously considered not endemic for this infection.</div><div>From April 2024 to June 2025, six mongrel dogs with poor body condition and skin lesions were presented to veterinary clinics in Hamedan, Iran. Impression smears and scrapes of the suspected lesions were examined microscopically for <em>Leishmania</em> amastigotes. In addition, spleen, skin, and lymph node samples from two dead dogs were collected for histological examination. Genomic DNA was extracted from skin lesions and further tested for <em>Leishmania</em> spp. DNA by conventional PCR and sequencing.</div><div>The infected dogs presented several clinical signs, including cachexia, muscle wasting, lymphadenopathy, muco−/cutaneous lesions, rough and dry hair coat, with alopecic regions characterized by hyperkeratosis, crusts and scales, desquamation, seborrheic dermatitis, and thick dandruff with bright reflections. <em>Leishmania infantum</em> amastigotes were observed in histological sections and/or impression smears of all dogs and further confirmed by DNA sequencing.</div><div>The presence of <em>L. infantum</em> in dogs from an area of Iran previously considered not endemic, spotlighting the importance of regular surveillance programs in canine populations for implementing control and preventive measures to reduce the risk of infection in both human and animal settings.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101351"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and distribution of piroplasms and Anaplasma species and their co-infections in cattle from three Egyptian governorates","authors":"Mohamed R. Hassan ,&nbsp;Hend H.A.M. Abdullah ,&nbsp;Reham S. El-Maadawy ,&nbsp;Sobhy Abdel-Shafy ,&nbsp;Hanem Khater ,&nbsp;Samah M. Abd Elgawad","doi":"10.1016/j.vprsr.2025.101350","DOIUrl":"10.1016/j.vprsr.2025.101350","url":null,"abstract":"<div><div>Tick-borne diseases (TBDs), including piroplasmosis and anaplasmosis, are globally distributed and pose significant threats to cattle. This study investigated the prevalence, co-infections, and risk factors of bovine piroplasmosis and anaplasmosis in three Egyptian governorates, Beni-Suef, Al-Faiyum, and Al-Minya, over a one year period. A total of 601 cattle blood samples were screened for hemopathogens using microscopic examination, Polymerase Chain Reaction (PCR), and nucleotide sequences analysis. Risk factors were analyzed using the chi-square (<em>χ</em>²) test (<em>P</em> &lt; 0.05). Hemopathogens were detected in 41.09 % and 75.04 % of cattle through microscopic and PCR methods, respectively. <em>Babesia bigemina</em> was the most prevalent pathogen (33.44 %, including both single and co-infections), followed by <em>Anaplasma marginale</em> (20.63 %), <em>Theileria annulata</em> (10.64 %), and <em>Babesia bovis</em> (3.49 %). Co-infections were observed in 19.8 % of cattle in all three studied governorates, with the most common combination being <em>A. marginale</em> and <em>B. bigemina</em> (63.86 %). Significant risk factors included seasonality (<em>B. bigemina</em> and <em>T. annulata</em>), breed (<em>B. bigemina</em>), and acaricide application (<em>A. marginale</em>). Phylogenetic analysis confirmed the presence of three piroplasm species and revealed two potentially distinct genotypes of <em>A. marginale</em>. This study is the first to report <em>B. bovis</em> in cattle from Al-Faiyum and Al-Minya, expanding its known distribution in Egypt. Multiplex PCR was successfully applied for simultaneous detection of multiple pathogens, supporting its utility for efficient, large-scale epidemiological studies. In conclusion, <em>B. bigemina</em> and <em>A. marginale</em> were the most prevalent pathogens and frequently co-occurred. The obtained results contribute to a better understanding of pathogen prevalence, distribution, and co-infection patterns and advocate for the design and implementation of region-specificcontrol strategies in endemic regions.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101350"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine Anaplasmoses in south and Southeast Asia: A systematic review","authors":"Vindya Perera ,&nbsp;Marina E. Eremeeva ,&nbsp;Ashoka Dangolla ,&nbsp;Shobhath Wijeratne ,&nbsp;Rupika S. Rajakaruna","doi":"10.1016/j.vprsr.2025.101349","DOIUrl":"10.1016/j.vprsr.2025.101349","url":null,"abstract":"<div><div>Canine anaplasmoses are tick-borne infections caused by two species of bacteria in the genus <em>Anaplasma. Anaplasma phagocytophilum</em> is the causative agent of granulocytic anaplasmosis with a global distribution and the etiological agent of human anaplasmosis while <em>A. platys</em> causes cyclic thrombocytopenia with a lower prevalence. This review offers a comprehensive and up-to-date analysis of canine anaplasmoses in South and Southeast Asia, a region where the disease has not received adequate attention. It provides detailed insights into the prevalence, geographical distribution, clinical signs, diagnostic methods, treatment strategies, risk factors, vectors, and zoonotic potential of canine anaplasmoses encompassing research published from 2000 to 2024. Seventy-one studies met the selection criteria. Infection with <em>A. platys</em> is more prevalent among dogs in the region, with cases reported in 13 countries, compared to <em>A. phagocytophilum</em>, which has been reported only in India and Malaysia. The infection rate of the two species varies widely between 1.3 % – 70.5 %. Concurrent infections with eight other canine pathogens were reported. Although <em>Anaplasma</em> DNA was detected in <em>Rhipicephalus sanguineus,</em> and four other tick species, specific biological vectors for <em>A. phagocytophilum</em> and <em>A. platys</em> have not been identified in these regions. Risk factors for canine anaplasmosis include sex, age, tick infestation, lack of tick-controlling methods, concurrent louse infestation, and climatic factors. Apart from 49 human cases of granulocytic anaplasmosis in India, extent of human infection remains largely unknown. Since most of the studies were limited to specific localities, accurately assessing the disease burden is a challenge. Overall, limited information is available on geographic distribution, prevalence, genetic diversity, vectors, and zoonotic potential of the pathogens in the region.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101349"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of Ancylostoma braziliense infection in southern Indian dogs: Evidence from molecular sequencing","authors":"Vijayashanthi Ramalingam ,&nbsp;Tirumurugaan Krishnaswamy Gopalan ,&nbsp;Sangaran Arumugam ,&nbsp;Samuel Masilamoni Ronald ,&nbsp;Jeyathilakan Narayanaperumal ,&nbsp;Raman Muthusamy","doi":"10.1016/j.vprsr.2025.101356","DOIUrl":"10.1016/j.vprsr.2025.101356","url":null,"abstract":"<div><div>Canine ancylostomiasis is an emerging soil-transmitted parasitic zoonosis that poses significant public and animal health concerns. Although <em>Ancylostoma caninum</em> and <em>A. ceylanicum</em> have been widely reported in India, molecular sequencing report on <em>A. braziliense</em> from Southern India remain limited. Therefore, the present study aimed to molecularly characterize the predominant canine hookworm species in dog faecal and soil samples in the urban community of Chennai, Tamil Nadu, India. A total of 278 dog faecal and 243 soil samples were examined through flotation techniques using saturated sodium chloride and sodium carbonate, respectively. The hookworm species were identified using ITS-based primers, followed by restriction endonuclease digestion using <em>HinFI</em>. Microscopic examination revealed that 42.5 % and 6.6 % hookworm positivity in faecal and soil samples, respectively. Molecular assays identified 75.5 % as <em>A. caninum</em>, 10.9 % as <em>A. braziliense</em> and 13.6 % as mixed infections with both species, with an overall prevalence of 93.2 % in faecal samples. The higher prevalence of hookworm infection in dogs less than one year of age compared to older dogs, with mongrels being more affected than purebred dogs. Owned dogs showed slightly higher infection rates than free-roaming dogs and male dogs were more commonly infected, especially during the monsoon. Phylogenetic analysis showed that <em>A. caninum</em> isolates clustered closely with those from Japan, Vietnam and China, indicating genetic relatedness and possible shared ancestry. <em>A. braziliense</em> isolates formed a distinct monophyletic clade, separate from isolates in Malaysia, Brazil and the USA, suggesting regional divergence and the presence of unique genetic lineages in Southern India.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101356"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial and seasonal analyses of tick prevalence in livestock across selected districts of Balochistan, Pakistan","authors":"Shan Masih ,&nbsp;Muhammad Arshad ,&nbsp;Haroon Ahmed ,&nbsp;Nida Aziz","doi":"10.1016/j.vprsr.2025.101357","DOIUrl":"10.1016/j.vprsr.2025.101357","url":null,"abstract":"<div><div>Ticks are ectoparasites that feed on the blood of domestic animals, resulting in significant reductions in weight, milk production, and hide quality. They are also vectors of a variety of pathogens that cause disease in animals. This study aimed to investigate the prevalence and species distribution of ticks among domestic animals (cattle, goats, sheep, buffaloes, and camels) across 12 districts of Baluchistan. A total of 759 domestic animals were carefully examined, and ticks were collected using hand and forceps. The collected samples were meticulously preserved in 70 % ethanol and appropriately labelled. All 308 collected ticks belong to two genera, <em>Hyalomma</em> (74.2 %) and <em>Rhipicephalus</em> (25.9 %), and seven species: <em>H. anatolicum, H. scupense, H. excavatum, H. dromedarii, R. appendiculatus, R. annulatus</em>, and <em>R. sanguineus sensu lato.</em> The overall tick prevalence was 41.37 % (314/759), with the highest prevalence recorded in cattle (57.14 %), followed by goats (40.93 %), sheep (32.18 %), buffaloes (32 %), and camels (25.66 %). There were significant (<em>p</em> &lt; 0.05) associations between tick infestation and factors, including host sex, age, breed, herd type, area, and season. A high tick burden impact animal productivity and increases the likelihood of tick-borne disease transmission. The findings of this study will help in the development and implementation of control measures for these parasites at the local and global levels.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101357"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}