Veterinary parasitology, regional studies and reports最新文献

{"title":"Seroprevalence and hematobiochemical changes associated with Toxoplasma gondii infection in goats from Khyber Pakhtunkhwa, Pakistan","authors":"Muhammad Iqbal ,&nbsp;Muhammad Yasin Tipu ,&nbsp;Asim Aslam ,&nbsp;Kamran Ashraf","doi":"10.1016/j.vprsr.2025.101373","DOIUrl":"10.1016/j.vprsr.2025.101373","url":null,"abstract":"<div><div>A cross-sectional study was conducted to determine the seroprevalence of <em>Toxoplasma gondii</em> (<em>T. gondii</em>) infection in goats and to evaluate associated haematological and biochemical alterations in Khyber Pakhtunkhwa, Pakistan. A total of 384 blood samples were randomly collected from goats in three districts and analyzed serologically using the latex agglutination test. The overall seroprevalence was 57.3 %, with the highest prevalence in Peshawar (60.2 %), followed by Dera Ismail Khan (57.8 %) and Swat (53.9 %). Higher infection rates were observed in female goats (66.1 %) and animals older than two years (71.4 %). Significant risk factors were female sex, age &gt; 2 years, grazing-based feeding, poor health status, and extensive rearing systems (<em>P</em> &lt; 0.05). Infected goats exhibited marked (P &lt; 0.05) haematological changes, including anemia, leukopenia, neutrophilia, and lymphocytopenia. Serum biochemistry revealed elevated liver enzymes (ALT, AST, LDH), as well as increased BUN and creatinine levels, alongside reduced albumin levels and increased total protein, indicating hepatic and renal involvement. The findings underscore the widespread occurrence of <em>T. gondii</em> infection in goats and its substantial impact on host physiology, with potential zoonotic implications. Priority interventions should focus on improving water hygiene, adopting controlled feeding systems, and implementing regular serological screening to effectively reduce <em>T. gondii</em> transmission and its impact on goat health.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101373"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145468174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrosequencing analysis of cytochrome b (CYTb) gene mutations in Babesia gibsoni: Correlation with drug resistance and treatment efficacy in South Korea","authors":"Galle Hettiarachchilage Ravindi Ashani Gunawardhana ,&nbsp;Doosung Cheon ,&nbsp;Woo-Jin Song ,&nbsp;Hyohoon Jeong ,&nbsp;YoungMin Yun","doi":"10.1016/j.vprsr.2025.101381","DOIUrl":"10.1016/j.vprsr.2025.101381","url":null,"abstract":"<div><div><em>Babesia gibsoni</em> is an intraerythrocytic tick-transmitted protozoan that presents a significant health risk to canine populations worldwide. Canine babesiosis is frequently associated with severe clinical symptoms, particularly hemolytic anemia. The present study aimed to determine the prevalence of mutations in the cytochrome <em>b</em> (<em>CYTb</em>) gene of <em>B. gibsoni</em> using pyrosequencing, and to explore potential correlations between specific mutations and treatment outcomes. The study was conducted at Jeju National University Animal Hospital, where canine patients diagnosed with <em>B. gibsoni</em> infection were evaluated. It is focused on five specific <em>CYTb</em> mutations: M121I, V220I, I303V, P152L, and F258Y. Genetic analysis using pyrosequencing was conducted on samples from infected dogs to identify the presence of these mutations. Various treatment regimens were administered based on clinical judgment, including atovaquone-azithromycin (AA), diminazene aceturate, metronidazole-clindamycin-doxycycline (MCD), and doxycycline-enrofloxacin-metronidazole (DEM). A total of 125 samples were used for mutation analysis, and only nine samples were assessed for the treatment response. The analysis revealed that the M121I mutation was present in 17 % of the <em>B. gibsoni</em>-positive dogs. Significant regional differences were observed in overall mutation prevalence among Jeju, Seoul and Gyeonggi area. Jeju samples showed a significantly higher mutation prevalence than those from PobAniLab. Dog sex, breed and concurrent disease were not significantly associated with the mutation prevalence. Overall, dogs with non-mutated strains tended to respond more favorably to initial treatments, whereas those with mutated strains often required more complex therapeutic approaches. This study supports the value of genetic monitoring in guiding treatment decisions and improving clinical outcomes of canine babesiosis.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101381"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of the giant kidney worm Dioctophyme renale in a domestic dog from Costa Rica","authors":"Fabián Villegas-Rojas ,&nbsp;Monserrat Barquero-Quesada ,&nbsp;Roberto W.I. Olivares ,&nbsp;Laura G. Bass ,&nbsp;Fernando Robles-Herrera ,&nbsp;Juan Carlos González-Barrantes ,&nbsp;Pablo J. Borrás ,&nbsp;Fernando A. Dittel-Meza ,&nbsp;Josué Campos-Camacho ,&nbsp;Alicia Rojas","doi":"10.1016/j.vprsr.2025.101387","DOIUrl":"10.1016/j.vprsr.2025.101387","url":null,"abstract":"<div><div><em>Dioctophyme renale</em>, the giant kidney worm, is a parasitic nematode of domestic and wild carnivores, occasionally infecting humans. While widely reported in South America, it has not been documented in Central America before. A 3-year-old French bulldog presented with leukocytosis, thrombocytopenia, azotemia, pyrexia, vomiting, and apathy. Abdominal ultrasound revealed tubular structures in the right kidney, and urine sediment contained nematode eggs. Euthanasia was performed after severe health complications, and a necropsy was done. The right kidney revealed severe hydronephrosis with parenchymal atrophy, fibrosis, and lymphoplasmacytic/eosinophilic nephritis. A 25 cm long worm was morphologically and molecularly analyzed, showing morphometric characteristics compatible with <em>D. renale.</em> Mitochondrial <em>cox</em>1 and ribosomal 18S and ITS1 sequences derived from the specimen were highly similar (&gt;98 %) to <em>D. renale.</em> Phylogenetic analysis of <em>cox</em>1 data clustered Costa Rican and Argentinian sequences irrespective of host and haplotype network revealed five geographically distributed haplotypes, with one shared between Argentina and Costa Rica. This study provides the first evidence of <em>D. renale</em> in Costa Rica, indicating local transmission risk. Considering the parasite's complex life cycle involving aquatic intermediate and paratenic hosts and the presence of susceptible wild carnivores in our country, environmental exposure represents a key risk factor. The findings highlight the need for a One Health approach integrating veterinary, public health, and environmental strategies to improve detection, prevention, and awareness of dioctophymosis in Central America.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"66 ","pages":"Article 101387"},"PeriodicalIF":1.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative susceptibility of native chicken breeds of Bangladesh to experimental Eimeria tenella infection","authors":"Dipesh Aryal,&nbsp;Kazi Farah Tasfia,&nbsp;Al Nur Tarak,&nbsp;Asmita Bhujel,&nbsp;Md. Shahiduzzaman","doi":"10.1016/j.vprsr.2025.101353","DOIUrl":"10.1016/j.vprsr.2025.101353","url":null,"abstract":"<div><div><em>Eimeria tenella</em> is the major protozoan parasite that causes coccidiosis in chickens, and its clinical and pathological response shows significant variations among breeds. This disease has a major economic impact on growers and the poultry industry worldwide. Very little information exists concerning the challenge infections of native chicken breeds of Bangladesh. The present study was undertaken to investigate and compare the pathology and clinical manifestations of experimental <em>E. tenella</em> infection among native (indigenous) chicken breeds of Bangladesh (Hilly, Naked Neck, and Deshi), in comparison to the susceptible exotic Sonali breed. In order to achieve this, a controlled experimental infection was carried out, and weight changes, Feed Conversion ratio (FCR), OPG counts, lesion severity, qPCR quantification of oocysts output, and dropping score were measured at 6, 10, and 14-Days post-infection (DPI). Distinct breed-specific differences in susceptibility were elicited. Sonali chickens exhibited the highest oocyst counts, reaching up to 103,200 at 14 DPI, along with significant weight loss and severe cecal pathology marked by diffuse hemorrhagic lesions. On the other hand, the indigenous breeds had low OPG counts as low as 100 in Hilly chickens, insignificant changes in weights, and mild pathological features characterized by reduced inflammation and hemorrhagic enteritis. This study highlights significant breed-specific differences in susceptibility to <em>E. tenella</em>, offering valuable insights into potential resistance or tolerance mechanisms. These findings support the use of native breeds in selective breeding programs aimed at improving disease resistance and underscore the importance of targeted control strategies for effective coccidiosis prevention and management.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101353"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A proposed strategic control approach for Dirofilaria immitis in Galápagos","authors":"Carla Andreea Culda ,&nbsp;Diego Páez-Rosas ,&nbsp;Rommel Lenin Vinueza ,&nbsp;Renato León ,&nbsp;Andrei Daniel Mihalca","doi":"10.1016/j.vprsr.2025.101361","DOIUrl":"10.1016/j.vprsr.2025.101361","url":null,"abstract":"<div><div>A strategic approach is proposed for controlling the invasive heartworm <em>Dirofilaria immitis</em> across the Galápagos Islands, where endemic fauna lacks natural resistance. This parasite poses a potential threat to the fitness of native wildlife, particularly the Galápagos sea lions (<em>Zalophus wollebaeki</em>), due to its interaction with the multi-host parasite <em>D. immitis</em>, domestic dogs and suitable mosquito vectors. The control program is based on a detailed understanding of the local life cycle and ecoepidemiology of the parasite. An active surveillance program is essential for successful control and should include testing of resident dogs and cats, passive surveillance, and monitoring of mosquito populations. All infected domestic carnivorans must be treated according to American Heartworm Society (AHS) guidelines, and mosquito control is crucial for reducing the risk of transmission. Additionally, collaboration, technology transfer, and institutional articulation between local entities and academia are essential. These initiatives enhance scientific understanding, improve practical applications, benefit communities, and promote sustainable development.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101361"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145363358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and risk factors for Acanthocheilonema reconditum infection in dogs in the municipality of Lages, Santa Catarina, Brazil","authors":"Faiane Reila Sousa Centenaro Duarte ,&nbsp;Geanice Ledo ,&nbsp;Felipe Rieth de Lima ,&nbsp;Maria Laura Correa Nunes ,&nbsp;Julia Milena Sandri Pereira ,&nbsp;Hanna Christna Antonia Brasil ,&nbsp;Ana Paula Remor Sebolt ,&nbsp;Larissa Américo ,&nbsp;Lúcia Padilha Cury Thomaz de Aquino ,&nbsp;Anderson Barbosa de Moura ,&nbsp;Andreas Lazaros Chryssafidis","doi":"10.1016/j.vprsr.2025.101352","DOIUrl":"10.1016/j.vprsr.2025.101352","url":null,"abstract":"<div><div><em>Acanthocheilonema reconditum</em> (syn. <em>Dipetalonema reconditum</em>) is a filarial nematode transmitted by arthropods (fleas, lice, and ticks), primarily parasitizing the subcutaneous tissue of canids, but also found in perirenal fat. The clinical relevance of <em>A. reconditum</em> is often overshadowed by <em>Dirofilaria immitis</em>, a more pathogenic nematode with which it can be easily confused during diagnosis There are no reports of <em>D. immitis</em> in Lages, Santa Catarina (SC), neither in the Santa Catarina Plateau, Brazil. However, local laboratories observed microfilariae in canine blood samples and requested identification by the Veterinary Parasitology Laboratory of the State University of Santa Catarina. Morphological evaluation confirmed <em>A. reconditum</em> larvae. This study aimed to determine the prevalence of <em>A. reconditum</em> infection and assess risk factors in dogs from Lages. A total of 444 blood samples were collected from dogs in different neighborhoods, with simultaneous coat inspection for ectoparasites. Information on age, sex, origin, breed, ectoparasite control, and presence or absence of clinical signs was obtained through an epidemiological questionnaire to assess risk factors for <em>A. reconditum</em> infection. Blood samples were analyzed by blood smear, modified Knott's test, and immunochromatographic test. The prevalence of <em>A. reconditum</em> was 22.07 % (98/444) by the modified Knott's test, whereas the blood smear detected only 4.27 % (19/444). None of the evaluated samples showed characteristics compatible with <em>D. immitis</em> in the Knott's test, and all tested negative in the immunochromatographic test, indicating the absence of circulating antigen of this species. Ectoparasites were found in 35.58 % (158/444) of the animals, with no general association as a risk factor for <em>A. reconditum</em> infection. Among ectoparasites, Siphonaptera predominated, present in 93.02 % of parasitized animals, with a significant association between <em>Pulex irritans</em> presence (<em>p =</em> 0.003) and <em>A. reconditum</em> infection. This study confirmed the absence of <em>D. immitis</em> in Lages and highlighted a high prevalence of <em>A. reconditum</em>, underscoring the zoonotic potential and the need for further investigations.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101352"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and epidemiological factors influencing Eimeria species occurrence in commercial chicken farms in Lagos State, Southwest Nigeria","authors":"Oluwayomi O. Adeyemi ,&nbsp;Mustapha F. Isaac ,&nbsp;Abigail O. Obi ,&nbsp;Razavi H. Hananeh ,&nbsp;Kim B. Stevens ,&nbsp;Damer P. Blake ,&nbsp;Isa D. Jatau ,&nbsp;Bamidele Akinsanya ,&nbsp;Emmanuel T. Idowu","doi":"10.1016/j.vprsr.2025.101359","DOIUrl":"10.1016/j.vprsr.2025.101359","url":null,"abstract":"<div><div><em>Eimeria</em> parasites, the causative agents of coccidiosis, are among the most economically significant pathogens in the global poultry industry. Understanding their epidemiology is important for effective control. This study determined the prevalence and risk factors of <em>Eimeria</em> parasites in commercial chicken farms in Lagos State, Nigeria. Between July and October 2020, pooled faecal/litter samples were collected from 265 chicken flocks across 157 randomly selected farms in Lagos-East (LE) and Lagos-West (LW) senatorial districts. Samples were screened microscopically for oocysts and quantified using the McMaster technique (oocysts per gram: opg). A subset of 30 samples positive for oocysts were analysed by PCR for species identification. Farm structure, management, and biosecurity practices were assessed via semi-structured questionnaires. <em>Eimeria</em> spp. were detected in 58.6% (92/157) of farms and 47.5% (126/265) of flocks, with a mean oocyst load of 5.2 x 10⁴ ± 2.2 x 10⁵ opg. The most common species detected was <em>Eimeria acervulina</em> (100.0%, 30/30) while <em>E. lata</em> (3.3%, 1/30) and <em>E. zaria</em> (3.3%, 1/30) were present but least common. LE farms had lower odds of infection (OR: 0.2, P &lt; 0.001) while those that housed multiple chicken types had a higher risk (OR: 5.0, P &lt; 0.001). At flock level, increased risk was associated with birds aged 6-10 weeks and placing feed in cages. Birds in battery cages had significantly lower oocyst counts than those on deep litter (β: 108,769.4, P = 0.006). This study highlighted the high prevalence of <em>Eimeria</em> in chicken farms in Lagos State and provided insights for improved control.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101359"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145363357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ixodes chilensis Kohls, 1956 (Acari: Ixodida: Ixodidae) in Argentina","authors":"Gabriel L. Cicuttin ,&nbsp;Patrick S. Sebastian ,&nbsp;María Alejandra Lombardo ,&nbsp;Marcela Simone ,&nbsp;María Nazarena De Salvo ,&nbsp;Santiago Nava","doi":"10.1016/j.vprsr.2025.101360","DOIUrl":"10.1016/j.vprsr.2025.101360","url":null,"abstract":"<div><div>The aim of this note is to report the first record of <em>Ixodes chilensis</em> Kohl, 1956 in Argentina. In June 2023, ticks were collected on bat belonging to the genus <em>Histiotus</em> from Dina Huapí, Rio Negro Province, Patagonian region, Argentina. Based on morphological characteristics and analysis of an obtained partial 16S rDNA gene sequence, these ticks (five nymphs) were determined as <em>I. chilensis</em>. This result represents the first record of this tick species outside Chile and expands the knowledge of its geographical distribution in the southern cone of South America.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101360"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of BoLA-DRB3 and its association with Anaplasma marginale and Babesia spp. infections in creole cattle of northeastern Colombia","authors":"Jeiczon Jaimes-Dueñez ,&nbsp;Laura Marin-Cossio ,&nbsp;Agustín Gongora-Orjuela ,&nbsp;Ian Murcia-Cueto ,&nbsp;Angela Jimenez-Leaño ,&nbsp;Olivia Marcuzzi ,&nbsp;Guillermo Giovambattista","doi":"10.1016/j.vprsr.2025.101355","DOIUrl":"10.1016/j.vprsr.2025.101355","url":null,"abstract":"<div><div>The <em>BoLA-DRB3</em> gene is one of the most polymorphic loci in cattle, with alleles associated with susceptibility or resistance to several infectious diseases, including leukosis, mastitis, and hemotropic infections. In this study, we assessed the genetic diversity of the <em>BoLA-DRB3</em> gene in three Colombian Creole cattle breeds and examined its association with natural infections by <em>Anaplasma marginale</em> and <em>Babesia</em> spp. in northeastern Colombia. A total of 97 animals (Chino – CrChi, <em>n</em> = 34; Casanareño - CrCAS-, <em>n</em> = 32; Sanmartinero - CrSM-, <em>n</em> = 31) from Arauca, Casanare, Meta, and Santander departments were genotyped using PCR–direct sequencing of the second exon of the <em>BoLA-DRB3</em> gene. Overall genetic diversity was moderate, with a nucleotide diversity of π = 0.086, a mean pairwise distance of 18.97, and 62 segregating sites. Among the breeds, CrCAS showed the highest diversity, followed by CrChi and CrSM. We identified 35 <em>BoLA-DRB3</em> alleles, 34 of which were previously reported in the IPD-MHC database, while one was novel. The CrChi population showed significant deviation from Hardy–Weinberg equilibrium. Two alleles, <em>BoLA-DRB3</em>*001:01 and <em>BoLA-DRB3</em>*025:01:01, were significantly associated with reduced risk of <em>B. bigemina</em> infection in CrSM, whereas <em>BoLA-DRB3*048:02</em> was linked to increased susceptibility to <em>B. bovis</em> in CrChi. This study confirms the high genetic variability of Colombian Creole cattle breeds. The high frequency of alleles associated with <em>B. bigemina</em> infection in CrSM highlights its potential as a genetic reservoir for future studies evaluating the heritability and functionality of these alleles. In contrast, the elevated frequency of a susceptibility-associated allele for <em>B. bovis</em> infection in CrChi, together with evidence of inbreeding, underscores the urgent need for targeted conservation and breeding strategies to preserve genetic diversity and reduce disease vulnerability to infectious diseases in this breed.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101355"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro acaricidal effects of essential oils against Rhipicephalus microplus via bioinformatics targeting of triosephosphate isomerase","authors":"Paola Cristina Colombo ,&nbsp;Arthur Gasetta Batista ,&nbsp;João Guilherme Martins ,&nbsp;Gabriel Nunes de Oliveira ,&nbsp;Marco Tulio da Silva Oliveira ,&nbsp;Giulia Poncione de Araujo ,&nbsp;Sabrina Nathalia Louzada Nogueira ,&nbsp;Rafael Paranhos de Mendonça ,&nbsp;Ricardo Andrade Furtado ,&nbsp;Isabela Cristina Gomes Honório ,&nbsp;Silvio de Almeida-Junior","doi":"10.1016/j.vprsr.2025.101354","DOIUrl":"10.1016/j.vprsr.2025.101354","url":null,"abstract":"<div><div>The tick <em>Rhipicephalus (Boophilus) microplus</em> poses a significant threat to animal health in tropical and subtropical regions, causing substantial economic losses in the meat and dairy industries. This study evaluated the potential of 20 essential oils as acaricidal agents, combining <em>in vitro</em> assays and <em>in silico</em> analyses to predict toxicity and efficacy. In the <em>in vitro</em> tests, the oils were assessed for their ability to reduce tick oviposition. The best results were observed at a 6 % concentration, with <em>Eucalyptus citriodora</em> showing the highest reduction (96.5 %), followed by <em>Betula lenta</em> (88.9 %), <em>Amyris balsamifera</em> (71.3 %), and <em>Cinnamomum cassia</em> (47.1 %). At a 3 % concentration, <em>Amyris balsamifera</em> (67.6 %) and <em>Betula lenta</em> (4.6 %) also showed significant efficacy, while the 1 % concentration had a limited impact on reducing oviposition, not meeting the legal efficacy criterion (&gt;95 %). Concurrently, <em>in silico</em> analyses were conducted using the SwissDock platform to predict the molecular interactions of natural compounds with specific biological targets. The results revealed the free binding energy (estimated ΔG) and the amino acid residues involved in the interactions, allowing for the comparison of the compounds' affinity with reference drugs. The data indicated that some compounds present in the essential oils demonstrated considerable target affinity, suggesting a promising mechanism of action against the tick's reproductive cycle. These findings highlight the potential of essential oils as natural alternatives for controlling <em>Rhipicephalus (Boophilus) microplus</em>, especially at higher concentrations. However, the efficacy at lower concentrations and the toxicity in host animals need further investigation before practical application.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"65 ","pages":"Article 101354"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}