Veterinary parasitology, regional studies and reports最新文献

{"title":"First morphological and molecular characterisation of Spirometra mansoni (Cestoda, Diphyllobothriidae) in a domestic cat from Veracruz, Mexico","authors":"Enrique Salazar-Grosskelwing ,&nbsp;Roger I. Rodriguez-Vivas ,&nbsp;Manuel E. Bolio-González ,&nbsp;Dora Romero-Salas ,&nbsp;Rodolfo Ramos-Beltrán ,&nbsp;Alberto Solano-Barquero ,&nbsp;Alicia Rojas","doi":"10.1016/j.vprsr.2024.101189","DOIUrl":"10.1016/j.vprsr.2024.101189","url":null,"abstract":"<div><div>Cestodes of the genus <em>Spirometra</em> are multi-host parasites that are the causative agents of spirometrosis in domestic and wild carnivores and sparganosis in humans, endemic diseases in tropical and subtropical regions. In domestic animals, the infection is usually asymptomatic or produces gastrointestinal signs such as vomiting and chronic diarrhoea. In humans, an incidental parasitosis develops where the plerocercoid can lodge in tissues and cause a variety of symptoms, including neuropathies, blindness, paralysis, and death. Reported here is a case of spirometrosis in a domestic cat treated in a veterinary clinic in Veracruz, Mexico. The cat presented with an approximate 50 cm section of the strobila protruding from the anus. The proglottids and eggs of the cestode were morphologically identified. DNA was obtained from the proglottids for the amplification and sequencing of a fragment of the cytochrome oxidase subunit 1 (<em>cox</em>1) gene. Genetic analysis demonstrated 100 % similarity to <em>Spirometra mansoni</em>. The cat was treated orally with ivermectin/praziquantel (2 mg/50 mg), and the dose was repeated 24 h later. The cat was observed daily for seven days, and no adverse effects occurred. On days 8 and 68 post-treatment, coprological examination revealed no evidence of the parasite. This is the first report of spirometrosis in cats from Mexico.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101189"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The parasitic fauna of the Chiloe wigeon Mareca sibilatrix (Anseriformes: Anatidae) from Ñuble region, Chile","authors":"Camilo H. Salazar-Silva ,&nbsp;Lucas Aravena ,&nbsp;Martín Zamorano-Uribe ,&nbsp;Javier Andrade-Hernández ,&nbsp;María Carolina Silva-de la Fuente ,&nbsp;Armando Cicchino ,&nbsp;Sergey Mironov ,&nbsp;Lucila Moreno ,&nbsp;Pablo Oyarzún-Ruiz","doi":"10.1016/j.vprsr.2025.101197","DOIUrl":"10.1016/j.vprsr.2025.101197","url":null,"abstract":"<div><div>The Chiloe wigeon, <em>Mareca sibilatrix</em> (Anseriformes: Anatidae), is a native duck distributed mainly in Chile and Argentina. Although it is a common species in the Neotropical realm, studies on its parasitic fauna remain limited. This study aimed to gather additional data on host-parasite associations in <em>M. sibilatrix</em>. Thus, 18 birds from Ñuble region, central Chile, were subjected to parasitic examination. Ectoparasites and endoparasites obtained were stored in 70 % and 80 % ethanol, respectively. A total of 787 parasite individuals were isolated; all birds were parasitized by at least one parasite. In 14 (77.7 %) birds, a total of 179 ectoparasites belonging to six taxa were identified. Meanwhile, all dissected birds (100 %) harbored a total of 608 endoparasites belonging to 15 taxa. Ectoparasites corresponded to four taxa of chewing lice (four genera) and two feather mites (two genera). Meanwhile, endoparasites were represented by four tapeworms (four genera), five trematodes (four genera), five nematodes (five genera), and one nasal mite. Of recorded parasite species, two taxa represent new records to the Neotropical realm, 11 taxa (three ectoparasites and eight endoparasites) are new records for the parasitic fauna of Chile, and 15 taxa (three ectoparasites and twelve endoparasites) are recorded for the first time in <em>M. sibilatrix</em>. Additionally, the fluke <em>Echinostoma echinatum</em> is of zoonotic concern. This research contributes with valuable data to the understanding of parasitic diversity in waterfowl and emphasizes the role of <em>M. sibilatrix</em> as a host in various parasitic interactions within its distribution range.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101197"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absolute eosinophil count and expression analyses of cytokine genes in Haemonchus contortus resistant Malpura sheep: Role of TH1/TH2 cytokine dichotomy","authors":"Rajiv Kumar ,&nbsp;Amar S. Meena ,&nbsp;Chander P. Swarnkar ,&nbsp;Siddharth S. Misra ,&nbsp;Arun Kumar","doi":"10.1016/j.vprsr.2025.101194","DOIUrl":"10.1016/j.vprsr.2025.101194","url":null,"abstract":"<div><div>A strong Th2-type immune response against <em>Haemonchus contortus</em> infection in genetically resistant sheep provide immunity. The objective of present study was to correlate mean faecal egg counts (FECs), absolute eosinophil counts and Th1/Th2 gene expression in resistant (R) and susceptible (S) Malpura sheep. In spite of no anthelmintic treatment in R line, on majority of the months, mean FECs remained significantly (<em>P</em> &lt; 0.05) lower compared to S line where anthelmintic treatment was given in September every year. R sheep possess a smaller number of blood eosinophils in the majority of months as compared with S sheep and a positive correlation has been observed between absolute eosinophil counts and mean FEC. In the condition, when FEC remain similar in both the lines (R and S), significantly (<em>P</em> &lt; 0.05) up-regulated expression of IL-5 was observed in R sheep compared with S sheep however when FEC is hugely enhanced; IL-2, IL-6, IL-12 and IL-13 gene expression was significantly (<em>P</em> &lt; 0.05) up-regulated in R sheep as compared to S sheep which is dependent on season and parasite load.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101194"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Culicoides and mosquito fauna at the National Zoological Garden of Rabat, Morocco","authors":"Maria Bourquia ,&nbsp;Claire Garros ,&nbsp;David Bru ,&nbsp;Hajar Chabih ,&nbsp;Fatine Bounaim ,&nbsp;Safae Annouri ,&nbsp;Saâd Azizi ,&nbsp;Mahmoud Zineeddine ,&nbsp;Abderrahmane Zahri ,&nbsp;Thomas Balenghien","doi":"10.1016/j.vprsr.2024.101181","DOIUrl":"10.1016/j.vprsr.2024.101181","url":null,"abstract":"<div><div>Zoos are considering to be essential places for the conservation of wild animal species. It is essential to prevent their infection by pathogens especially for those belonging to threatened or extinct species. Zoo captive animals are susceptible to several <em>Culicoides</em> and mosquito borne-viruses. In order to further evaluate the risk of pathogen transmission in zoos, it is essential to identify the presence of potential vector species, as well as the animals bitten by those vectors.</div><div>For this purpose, <em>Culicoides</em> and mosquito species composition was investigated in ten sites from March to June 2021 for <em>Culicoides</em> (18 collection nights) and in four sites from April to June 2022 for mosquitoes (16 collection nights) at the National Zoological Garden of Rabat (ZGR), Morocco. <em>Culicoides</em> (Diptera: Ceratopogonidae) were collected using Onderstepoort Veterinary Institute traps (UV-light/suction traps (OVI type)), every two weeks. Mosquitoes (Diptera: Culicidae) were collected using BG-Pro mosquito trap (BGP) combined with a CO2 source as an attractant, on two consecutive days every two weeks. The blood meal of engorged <em>Culicoides</em> was amplified by PCR, sequenced, and blasted for host species identification.</div><div>In total, 1584 individuals belonging to the <em>Culicoides</em> genus were collected (88.25 % females and 11.75 % males) belonging to at least 13 different species. Among the species collected, <em>Culicoides newsteadi</em> (33.28 %), <em>C. imicola</em> (23.74 %), <em>C. circumscriptus</em> (18.88 %) and <em>C. obsoletus/C. scoticus</em> (7.96 %) constituted the majority of the total catches. These species are proven or suspected vector species of bluetongue and African hose sickness viruses in the Mediterranean basin. For mosquitoes, 455 individuals belonging to four species of three genera were collected (97.58 % females and 2.42 % males): <em>Culex pipiens</em> s.l. (94.29 %) (vector species of West Nile and Rift Valley fever viruses)<em>, Culiseta longiareolata</em> (4.81 %)<em>, Aedes detritus</em> s.l. and <em>Ae. caspius</em> (representing together less than 1.00 %). The results of blood meal analyses revealed that <em>Culicoides</em> fed on humans (<em>n</em> = 7), camels (<em>n</em> = 2), and common eland (<em>n</em> = 2).</div><div>The composition of <em>Culicoides</em> and mosquito fauna is characteristic of the Rabat region. The composition is thus mostly determined by the environment rather than by the animal species presence. The results highlighted that <em>Culicoides</em> fed on humans and ruminants. It is therefore likely that the zoo's animals could be threatened by arboviruses transmitted by domestic animals in the region. Particular attention must be paid to the prevention of vector-borne diseases to ensure the proper conservation of species.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101181"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generalized knemidokoptic mange (Micnemidocoptes sp.) in a bald eagle (Haliaeetus leucocephalus) from Arkansas, USA","authors":"Jomari Rivera Reyes ,&nbsp;C. Robert Stilz ,&nbsp;Raquel Francisco ,&nbsp;Jillian R. Broadhurst ,&nbsp;Taylor A. Pearson ,&nbsp;Nicole M. Nemeth ,&nbsp;Victoria A. Andreasen ,&nbsp;Jennifer R. Ballard ,&nbsp;Samantha E.J. Gibbs ,&nbsp;Thomas Young ,&nbsp;Michael J. Yabsley","doi":"10.1016/j.vprsr.2024.101187","DOIUrl":"10.1016/j.vprsr.2024.101187","url":null,"abstract":"<div><div>This report describes a case of generalized knemidokoptic mange caused by <em>Micnemidocoptes</em> sp. in a bald eagle (<em>Haliaeetus leucocephalus</em>). In June 2024, an adult male eagle from Polk County, Arkansas, presented with severe skin disease and in a moribund state. The eagle died soon after capture and postmortem examination revealed extensive skin hyperkeratosis with embedded mites. Molecular analysis of a 632 bp fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene showed the mite was 99.8 % similar to a <em>Micnemidocoptes</em> sp. from a golden eagle (<em>Aquila chrysaetos)</em> in California and these mites grouped together. This represents a large geographic expansion in the known range of this mite in the United States and a novel host. This case highlights the potential health impact of <em>Micnemidocoptes</em> sp. infestations in raptors, emphasizing the need for increased monitoring among eagles and other raptors to better understand the mite's epidemiology and implications for wildlife health.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101187"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection with the zoonotic nematode, Trichinella murrelli in bobcats (Lynx rufus) from Minnesota, USA","authors":"Jitender P. Dubey ,&nbsp;Aditya Gupta ,&nbsp;Peter C. Thompson ,&nbsp;Benjamin Rosenthal ,&nbsp;Carolin Humpal ,&nbsp;Michelle Carstensen","doi":"10.1016/j.vprsr.2024.101188","DOIUrl":"10.1016/j.vprsr.2024.101188","url":null,"abstract":"<div><div>During a survey for other parasitic infections, tongue and limb muscles of wild bobcats (<em>Lynx rufus</em>) were examined microscopically from a sample of 190 Minnesota bobcats. One batch (<em>n</em> = 50) was studied in 2015, yielding no cases of <em>Trichinella</em> when examined only with hematoxylin and eosin (HE) staining of &lt;25 mg of tissue<em>.</em> In a 2024 cohort (<em>n</em> = 140), this method was paired with a compression of ∼5 g of tissue. Although these methods were not perfectly suited to the detection of <em>Trichinella</em>, such larvae were nonetheless identified in one specimen. Genetic data confirmed these as <em>Trichinella murrelli,</em> representing the first identification of this species from any host from Minnesota.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101188"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemosporidian infection in free-living Cathartes aura (Linnaeus, 1758) in the Amazon biome, Pará State, Brazil: Parasitological, molecular, hematological, and biochemical analyses","authors":"Nailson de Andrade Neri Júnior ,&nbsp;Alessandra Scofield ,&nbsp;Anna Klara de Matos Guerreiro ,&nbsp;Amanda de Nazaré da Costa Rodrigues ,&nbsp;Analiel Serruya ,&nbsp;Thyele Chaves da Silva ,&nbsp;Leonardo Carvalho Costa ,&nbsp;Dallyth Maia da Costa Santos ,&nbsp;Felipe Sousa Furtado da Silva ,&nbsp;Rodrigo Corrêa Sousa ,&nbsp;Paulo Cesar Magalhães Matos ,&nbsp;Cinthia Távora de Albuquerque Lopes ,&nbsp;Natália da Silva e Silva Silveira","doi":"10.1016/j.vprsr.2024.101191","DOIUrl":"10.1016/j.vprsr.2024.101191","url":null,"abstract":"<div><div>Hemosporidians are intracellular pathogens that infect a range of endothermic and ectothermic hosts and are transmitted by dipteran vectors. These parasites are grouped into four families: Haemoproteidae, Plasmodiidae, Leucocytozoidae, and Garniidae, with the genera <em>Haemoproteus</em>, <em>Plasmodium</em>, and <em>Leucocytozoon</em> being the most common in birds. Therefore, the objectives of this study were to detect the infection frequency of hemosporidians and to perform hematological and biochemical analyses in free-living turkey vultures (<em>Cathartes aura</em>) in the Amazon biome, Brazil. Blood samples were collected from free-living <em>C. aura</em> specimens captured by the wildlife management team at Júlio Cezar Ribeiro International Airport (Val-de-Cans) in Belém, Pará, Brazil. A total of 26<em>C. aura</em> specimens were examined. Parasitological and molecular analyses were conducted to detect hemosporidian infection, and hematological and biochemical analyses were also performed on all birds. Parasite forms suggestive of hemosporidians were observed in blood smears from 42.31 % (11/26) of the birds, while molecular analyses indicated 76.92 % (20/26) of the animals were positive. There was no significant difference in hematological values between the animals infected and those not infected by hemosporidians. In serum biochemistry, there was a significant difference in the albumin/globulin ratio, with higher values in uninfected vultures. It is concluded that <em>Haemoproteus</em> spp./<em>Plasmodium</em> spp. infections occur in <em>C. aura</em> in the Amazon biome. These infections did not cause evident deleterious effects on the health of the examined animals based on hematological results, but there were changes in the albumin/globulin ratio between infected and uninfected groups.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101191"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Amblyomma maculatum and Rickettsia parkeri in prairies of Central Missouri","authors":"Jaylon Vaughn ,&nbsp;Haylie J. Brown ,&nbsp;Anthony Ogunbadewa ,&nbsp;Solomon Odemuyiwa ,&nbsp;Deborah M. Anderson ,&nbsp;Samniqueka J. Halsey","doi":"10.1016/j.vprsr.2024.101190","DOIUrl":"10.1016/j.vprsr.2024.101190","url":null,"abstract":"<div><div>The destruction and decline of prairie habitats due to landscape repurposing have profoundly impacted the diversity of plant, animal, and insect life. In the Central United States, the reconstruction of prairie habitats from farmland is a widely applied strategy to raise diversity and recreate a healthy, complex ecosystem. In Central Missouri, we examine the consequences of reconstruction efforts on the prevalence of zoonotic diseases and their associated pathogens by performing tick-flagging at two prairie sites. In doing so, we have observed large populations of Dog ticks, <em>Dermacentor variabilis</em>, and Lone Star ticks, <em>Amblyomma americanum</em>, as well as the first recorded instance of the Gulf Coast tick, <em>Amblyomma maculatum,</em> in Callaway County. The Gulf Coast tick is traditionally found mainly along the Atlantic and Gulf Coast regions but has been undergoing geographic expansion in the last decade. Since detection in 2020, the number of Gulf Coast ticks has increased. Upon screening 193 Gulf Coast ticks in 105 pools via PCR, we found that 6.67 % of ticks were positive for pathogenic <em>Rickettsia parkeri,</em> which causes spotted fever rickettsiosis in animals and humans, with most ticks coming from the reconstructed prairie site. These observations suggest that reconstruction of prairie habitat has the potential to support the sylvatic cycle of <em>Rickettsia parkeri</em>. Collectively, our observations show that the reconstructed prairie are capable of harboring large tick populations as compared to remnant prairies and demonstrates a potential increase in disease risk as a result. This works highlights the importance of proactive surveillance of ticks, especially with land-use change.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101190"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of Leishmania spp. in phlebotomine sand flies and dogs in Guelma region, North-eastern Algeria","authors":"Ilham Ferdes ,&nbsp;Maria Stefania Latrofa ,&nbsp;Livia Perles ,&nbsp;Marcos Antonio Bezerra-Santos ,&nbsp;Bachir Medrouh ,&nbsp;Meryem Benmarce ,&nbsp;Karim Ouachek ,&nbsp;Ahcène Hakem ,&nbsp;Ismail Lafri ,&nbsp;Domenico Otranto","doi":"10.1016/j.vprsr.2024.101176","DOIUrl":"10.1016/j.vprsr.2024.101176","url":null,"abstract":"<div><div><em>Leishmania</em> spp. are sand fly-borne parasitic protozoa of worldwide distribution that may severely affect the health and welfare of dogs as well as of other mammalian species, including humans. Algeria is among the most affected countries, counting several cases of <em>Leishmania infantum</em> infection in humans and dogs. Here, we assessed the occurrence of <em>Leishmania</em> species in both <em>phlebotomine</em> sand fly and dogs in the Guelma region, Northeast of Algeria. Sand flies were collected from July to September 2022, followed by a survey of canine leishmaniasis (CanL) from September 2023 to February 2024. Additionally, to understand the risk of human infection a retrospective data on cases of leishmaniasis recorded in the region from 2012 to 2023 were reported. Sand fly specimens and canine blood samples were tested by quantitative PCR (qPCR) for <em>Leishmania</em> spp., while dog serum samples were processed for anti-<em>L. infantum</em> antibodies detection by immunofluorescent antibody test (IFAT). Of the 1478 sand flies (<em>n</em> = 318 females; <em>n</em> = 1160 males), seven species were identified (i.e., <em>Phlebotomus papatasi, Ph. perfiliewi, Ph. sergenti, Ph. perniciosus, Ph. longicuspis, Ph. ariasi,</em> and <em>Sergentomyia minuta). Leishmania</em> spp. was detected in <em>Ph. perniciosus</em>, <em>Ph. perfiliewi</em>, <em>Ph. papatasi,</em> and <em>Ph. sergenti</em> (i.e., 3.7 %; <em>n</em> = 10). The overall seroprevalence rate was 58.2 %, with 1.6 % of dog blood samples positive for <em>L. infantum</em> at molecular screening. Multivariate analysis of the different risk factors revealed that CanL seropositivity was strongly related to dog age (&gt; 1 year) (OR = 8.35, 95 % CI: 3.43–23.93), the autumn season (OR = 2.95, 95 % CI: 1.33–8.62), and lack of insecticide application (OR = 6.69, 95 % CI: 2.61–22.02). A total of 71 human cases of cutaneous (CL) and visceral (VL) leishmaniasis were recorded. Data presented reveal the occurrence of CanL in Guelma region, advocating for control program measures to be implemented in this part of Algeria.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101176"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of selected vector-borne pathogens in domestic animals, ectoparasites, and their owners in a rural community in Southwest Guatemala","authors":"A. Valeria Scorza ,&nbsp;Rebekah J. McMinn ,&nbsp;Andrea Chacon ,&nbsp;Molly M. Lamb ,&nbsp;Ramon E. Medrano ,&nbsp;Emma K. Harris ,&nbsp;Danilo Alvarez ,&nbsp;Maria Renee Lopez ,&nbsp;Kareen Arias ,&nbsp;Jose Anaya ,&nbsp;Daniel Olson ,&nbsp;Gregory D. Ebel ,&nbsp;Michael R. Lappin","doi":"10.1016/j.vprsr.2024.101185","DOIUrl":"10.1016/j.vprsr.2024.101185","url":null,"abstract":"<div><div>Vector-borne pathogens, which are transmitted by blood-feeding arthropods to animals and people, are common in tropical regions where, combined with economic factors, can cause significant public health burden. A community-level study was undertaken in southwestern Guatemala to assess the presence of vector-borne pathogens in blood samples from humans (<em>n</em> = 98), their animals (<em>n</em> = 90), and ectoparasites (<em>n</em> = 83) over a period of 2 weeks. Human capillary blood was collected from participant's index finger, and animal venous blood (chickens, pigs, dogs, and cats) was collected from the jugular or cephalic veins at the enrollment period of a concurrent study. Ectoparasites (fleas, ticks, and lice) were collected from dogs at the time of the blood collection. Total DNA was extracted from the human blood, animal blood, and ectoparasites and assayed using published PCR assays for <em>Anaplasma</em> spp., <em>Bartonella</em> spp., and <em>Ehrlichia</em> spp. Ectoparasites were also tested for the presence of <em>Rickettsia</em> spp. DNA by PCR. <em>Anaplasma</em> spp. DNA was amplified from 1 of 39 (2.6 %) chickens and 1 of 6 (16.6 %) turkeys. All human and dog blood samples were negative for <em>Bartonella</em> spp. in the same community. <em>Ehrlichia</em> spp. DNA was amplified from 12 (60 %) of 20 dogs and sequencing documented <em>Ehrlichia</em> spp. in 2 dogs and the ticks and fleas collected from these dogs. All the <em>Ehrlichia</em> spp.-positive sequences showed 100 % homology to <em>E. canis</em> sequences and other uncultured <em>Ehrlichia</em> spp. strains isolated from animals. <em>Rickettsia</em> spp. DNA was not amplified from any of the ectoparasites assessed. Our findings suggest that <em>Ehrlichia</em> spp. are common in dogs and <em>Anaplasma</em> spp. are circulating in poultry in a rural community in southwest Guatemala. We expect these results to be used in awareness campaigns and public health interventions to reduce vector borne pathogens in the region.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101185"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}