Veterinary parasitology, regional studies and reports最新文献

{"title":"Ovine dicrocoeliosis in Castellón province, eastern Spain: Prevalence, dynamics, and comparative study of different diagnostic techniques","authors":"Paula Redondo-Pérez ,&nbsp;José Sansano-Maestre ,&nbsp;Pedro María Alarcón-Elbal ,&nbsp;Ana Elena Ahuir-Baraja ,&nbsp;Alejandra Escudero-Cervera ,&nbsp;María Magdalena Garijo-Toledo","doi":"10.1016/j.vprsr.2024.101073","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101073","url":null,"abstract":"<div><p>Dicrocoeliosis is a parasitic disease that mainly affects ruminants during grazing, caused by trematodes of the genus <em>Dicrocoelium</em>, with <em>D. dendriticum</em> being the most common species worldwide. This parasitosis is a chronic and generally subclinical process, with nonspecific signs, which makes its diagnosis challenging. This study aimed to determine the prevalence and seasonal dynamics of <em>D. dendriticum</em> infection in adult sheep from the Valencian Community, eastern Spain, as well as to evaluate the efficacy of flotation and sedimentation techniques when compared with the macroscopic exam of the liver. From February to May 2018, 2019, 2020, 2021, and 2022, a total of 290 adult sheep were examined. The animals were sourced from Castellón province, where a semi-intensive production system predominates. Each animal was euthanized and underwent a macroscopic examination of the liver, as well as a fecal analysis. Among the sampled animals, 117 (40.6%) tested positive for adult trematodes in their liver, while 87 (30%) showed evidence of trematode eggs in the coprological exam, reaching a total of 126 sheep parasitized, with a prevalence of 43.4%. The parasitic burden was established in 90.3 adults per animal when the liver was examined and in 54.5 eggs per gram when the sedimentation coprological exam was performed. No eggs were observed when the flotation technique was employed. A positive correlation was found between the number of adults in the liver and the fecal egg count. No significant differences were detected in the prevalence or parasitic burden throughout the study. Considering the difficulty in controlling the intermediate hosts and the complexity of the life cycle, effective diagnostic methods, combined with the adoption of other preventive measures, is crucial to achieving proper management of this parasitic disease.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101073"},"PeriodicalIF":1.4,"publicationDate":"2024-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of a topical application of moxidectin in bare-nosed wombats (Vombatus ursinus)","authors":"Hayley J. Stannard ,&nbsp;Marie B. Wynan ,&nbsp;Ray J. Wynan ,&nbsp;Amanda Cox ,&nbsp;Howard Ralph ,&nbsp;Gregory S. Doran","doi":"10.1016/j.vprsr.2024.101074","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101074","url":null,"abstract":"<div><p>Sarcoptic mange is a debilitating disease that affects bare-nosed wombats (<em>Vombatus ursinus</em>). One of the drugs currently used for treatment is moxidectin, as it has a relatively high efficacy against endo and ectoparasites and side effects are uncommon in domestic species, thus it is considered a relatively safe drug to use at the recommended doses. Developing further understanding of the pharmacokinetics of moxidectin will aid in developing treatment regimens for sarcoptic mange in wombats. Here we analyzed the pharmacokinetic parameters of using 100 ml of moxidectin (5 g/l) applied topically. We found that mean peak plasma concentration was 0.50 ng/ml and half-life was 8 days. Moxidectin was excreted in scats with the mean peak concentration of 2461.43 ng/g (on a dry matter basis). Our study has provided the pharmacokinetic parameters of a commonly used treatment for sarcoptic mange in wombats. There were no adverse side effects recorded in the wombats after applying moxidectin topically. This study replicated real-world conditions using topical application on free-living wombats. The relatively low plasma concentration suggests the drug is not accumulating in the blood stream and is excreted via scats.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101074"},"PeriodicalIF":1.4,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405939024000947/pdfft?md5=2da1bb07562f9ba17deb1dae2fbfb4ad&pid=1-s2.0-S2405939024000947-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141539661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of opisthorchiid and other endoparasitic infections among cats and dogs in Novosibirsk oblast (Western Siberia, Russia)","authors":"Sergey Konyaev ,&nbsp;Natalia Ponomareva,&nbsp;Elena Serbina,&nbsp;Yuri Prilepsky,&nbsp;Anton Krivopalov,&nbsp;Natalia Yurlova","doi":"10.1016/j.vprsr.2024.101075","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101075","url":null,"abstract":"<div><p>To determine the current reservoir status and prevalence of <em>Opisthorchis felineus</em> infection in opisthorchiosis-endemic areas, feces samples were collected from 103 cats and 101 dogs admitted to shelters or discount sterilization centers from various districts of Novosibirsk city and from villages and towns along the Ob River (Novosibirsk Oblast). The feces samples were evaluated using two methods simultaneously: the formalin–ether sedimentation assay and a modified flotation assay. The prevalence of opisthorchiid infection was higher among cats (12.6%; 95% confidence interval 6.9–20.6%) than among dogs (4.0%; 95% confidence interval 1.1–9.8%). Overall prevalence of endoparasites among cats was 42.7% (five helminthic and four protozoan species) and among dogs 51.5% (eight helminthic and five protozoan species). Among the parasites found in the dogs, the prevalence of <em>Sarcocystis</em> sp. was the highest (16.8%), and in cats, the highest prevalence was shown by <em>Giardia</em> sp. and <em>Toxocara cati</em> (14.6% for each species). These results confirm the considerable contribution of stray cats and dogs to the maintenance of opisthorchiid eggs in the environment; these data should be taken into account for the development of effective control and education programs.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101075"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of Cryptosporidium species from diarrhoeic ungulates in the United Arab Emirates","authors":"M. Procter ,&nbsp;S. Savikumar ,&nbsp;L. Hamdan ,&nbsp;S. Al Naqbi ,&nbsp;M. Kváč ,&nbsp;R.K. Schuster ,&nbsp;M.A. Qablan","doi":"10.1016/j.vprsr.2024.101067","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101067","url":null,"abstract":"<div><p>Cryptosporidiosis has previously been reported in animals, humans, and water sources in the United Arab Emirates (UAE). However, most reports were only to the genus level, or generically identified as cryptosporidiosis. We aimed to investigate the genetic diversity of <em>Cryptosporidium</em> species occurring in diarrhetic ungulates which were brought to the Central Veterinary Research Laboratory (CVRL) in Dubai. Using a combination of microscopic and molecular methods, we identified five species of <em>Cryptosporidium</em> occurring among ungulates in the UAE, namely <em>C. parvum</em>, <em>C. hominis</em>, <em>C. xiaoi</em>, <em>C. meleagridis</em>, and <em>C. equi</em>. <em>Cryptosporidium parvum</em> was the most prevalent species in our samples. Furthermore, we identified subtypes of <em>C. parvum</em> and <em>C. hominis</em>, which are involved in both human and animal cryptosporidiosis. This is also the first reported occurrence of <em>Cryptosporidium</em> spp. in the Arabian Tahr, to our knowledge. Since the animals examined were all in contact with humans, the possibility of zoonotic spread is possible. Our study correlates with previous reports in the region, building upon the identification of <em>Cryptosporidium</em> sp. However, there is a need to further investigate the endemic populations of <em>Cryptosporidium</em>, including more hosts, sampling asymptomatic animals, and location data.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"54 ","pages":"Article 101067"},"PeriodicalIF":1.4,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141607671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of occurrence of Babesia gibsoni in captive Indian wolves","authors":"N. Madan ,&nbsp;P. Azhahianambi ,&nbsp;R.P. Aravindh Babu ,&nbsp;Neelanjana Gayen ,&nbsp;K.G. Tirumurugaan ,&nbsp;R. Sridhar ,&nbsp;C. Soundararajan","doi":"10.1016/j.vprsr.2024.101071","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101071","url":null,"abstract":"<div><p>Blood samples from fifteen captive Indian wolves (<em>Canis lupus pallipes</em>) maintained at Arignar Anna Zoological Park, Vandalur, Chennai were screened for the presence of <em>Babesia</em> spp., <em>Ehrlichia canis</em> and <em>Trypnosoma evansi</em> DNA by PCR. Out of 15 wolf samples, 3 samples were found positive for <em>Babesia</em> spp. The amplified <em>18S rRNA</em> gene fragments from 3 wolves were sequenced and confirmed as <em>Babesia gibsoni</em>. A maximum likelihood tree was constructed using the three sequences along with other <em>Babesia</em> spp. sequences derived from GenBank adopting HKY nucleotide substitution model based on the Bayesian Information Criterion. The phylogenetic analysis confirmed that the three sequences were of <em>Babesia gibsoni</em> and highly divergent from <em>Babesia canis, B. vogeli</em> and <em>B. vulpes.</em> This might be a possible spill over event of <em>B. gibsoni</em> from community dogs through blood feeding dog ticks. This is the first report and molecular confirmation of <em>B. gibsoni</em> infection in captive Indian wolves.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101071"},"PeriodicalIF":1.4,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report on seroprevalence of Toxoplasma gondii infection in zoo animals in Algeria","authors":"Imane Ouchetati ,&nbsp;Nadjet Amina Khelifi Touhami ,&nbsp;Ilyes Bouasla ,&nbsp;Nassim Ouchene","doi":"10.1016/j.vprsr.2024.101072","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101072","url":null,"abstract":"<div><p><em>Toxoplasma gondii</em> is one of the world's most widespread polyxenic protozoan parasites that affect all warm-blooded animals, including humans. This survey aims to study, for the first time in Algeria, the seroprevalence of <em>Toxoplasma</em> infection in zoo animals. The study included eight animal species of which 54 serum samples were collected from 30 Australian goats (<em>Capra hircus</em>), four bulls (<em>Bos taurus</em>), one dromedary (<em>Camelus dromedarius</em>), three cuffed sheep (<em>Ammotragus lervia</em>), seven donkeys (<em>Equus asinus</em>), one pony (<em>Equus ferus</em>), four bearded horses (<em>Equus ferus caballus</em>) and four rabbits (<em>Oryctolagus cuniculus</em>). The presence of antibodies to <em>T. gondii</em> was determined using the ID Screen® Toxoplasmosis Indirect Multispecies ELISA kit (IDVet, Grabels, France). A total of 8/54 (14.8%) samples were seropositive, including 5/28 (17.9%) males and 3/26 (11.5%) females. The seroprevalence was 6.7%, 50%, 25% and 75% in <em>Capra hircus</em>, <em>Bos Taurus, Equus ferus caballus,</em> and <em>Oryctolagus cuniculus</em>, respectively. No cases were observed in <em>Camelus dromedarius, Ammotragus lervia, Equus asinus,</em> and <em>Equus ferus</em>. This study indicates, for the first time in Algeria, the seroprevalence of <em>T. gondii</em> in zoo animals.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101072"},"PeriodicalIF":1.4,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141485261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrointestinal nematodes in Cuniculus paca (Linnaeus, 1766) from hunting fauna in the Western Amazonian region","authors":"Victor Silva Vasconcelos ,&nbsp;Francisco Emerson Feitosa Lobo ,&nbsp;Ademar Guimarães de Souza Neto ,&nbsp;Maria Isabel Afonso da Silva ,&nbsp;Lucena Rocha Virgilio ,&nbsp;Matheus Nascimento Oliveira ,&nbsp;Rodrigo Lima do Nascimento ,&nbsp;Manuela Jucá Correa ,&nbsp;Felippe Bissagio Pereira ,&nbsp;Dirceu Guilherme de Souza Ramos ,&nbsp;Tiago Lucena da Silva","doi":"10.1016/j.vprsr.2024.101066","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101066","url":null,"abstract":"<div><p><em>Cuniculus paca,</em> commonly known as the paca, is a rodent of the Cuniculidae family that is widely distributed throughout the Americas, including all Brazilian territories, and is abundant in the Amazon region. It is one of the most hunted species and faces significant predation in the extreme western Amazon region of Brazil because it constitutes a staple in the diet of local communities, for subsistence and commercial purposes. Understanding the helminthic fauna of these animals is of paramount importance, given that some nematodes have zoonotic potential and may pose risks to consumer health. This study aimed to contribute to the records of the nemtodes of this highly consumed species in the Amazon region, highlighting the occurrence of gastrointestinal parasites in free-living pacas intended for human subsistence consumption. The study was conducted in the Paranã da Floresta community, located in the municipality of Guajará, Amazonas from 2022 to 2023. The community members hunted this rodent for consumption and voluntarily provided the viscera for analysis. Nematodes were extracted from each organ. The organs were opened, and the contents were processed using a sieve (0.15 mm) and subsequently evaluated separately. In total, 10,157 nematodes were found in the 14 pacas. Based on morphological analyses, the nematodes were identified as <em>Heligmostrongylus sedecimradiatus</em> (<em>n</em> = 10,068), <em>Trichuris</em> sp. (<em>n</em> = 85), and <em>Physaloptera</em> sp. (<em>n</em> = 4). This study provides insights into the nematodes diversity of free-living pacas in the extreme Western Amazon region, emphasizing the importance of sanitary surveillance and public awareness of the risks associated with bushmeat consumption.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101066"},"PeriodicalIF":1.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Leishmania infection in 205 cats from a referral hospital population in Brazil (2021−2022)","authors":"Brenda Lopes Paiva ,&nbsp;Lucas Christian de Sousa-Paula ,&nbsp;Kamila Gaudêncio da Silva Sales ,&nbsp;Karla Maria Vieira Costa ,&nbsp;Aline Martins Venuto ,&nbsp;Valcledes Nascimento do Oriente ,&nbsp;Francisco Roger Aguiar Cavalcante ,&nbsp;Roberta Lomonte Lemos de Brito ,&nbsp;Jessica Maria Leite dos Santos ,&nbsp;Filipe Dantas-Torres","doi":"10.1016/j.vprsr.2024.101068","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101068","url":null,"abstract":"<div><p>Leishmaniases are a group of neglected diseases of significant public health concern, with Brazil being the primary focus of this disease in the Americas. The municipality of Sobral, in the state of Ceará, is a historical focus of visceral leishmaniasis in both humans and dogs, but data on <em>Leishmania</em> spp. infections in cats are limited. Between April 2021 and February 2022, 205 cats from a referral hospital population were sampled and tested for <em>Leishmania</em> spp. by real-time PCR. Eight cats (3.9%; 95% CI: 1.7–7.5%) tested positive. Among these, three (37.5%) displayed clinical signs compatible with feline leishmaniosis. Non-domiciled cats showed significantly higher positivity compared to domiciled ones (Fisher's exact test, <em>P</em> = 0.0124). Considering their potential role as reservoirs of <em>L</em>. <em>infantum</em>, it is crucial to conduct further studies to understand the <em>Leishmania</em> spp. circulating among cats in Sobral and to implement measures for reducing their exposure to phlebotomine sand fly vectors in this important focus of leishmaniases.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101068"},"PeriodicalIF":1.4,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141482203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and prevalence of ticks infesting small ruminants of Fort Munro region in South Punjab, Pakistan","authors":"Basat Latif ,&nbsp;Muhammad Naeem ,&nbsp;Muhammad Nadeem Khan ,&nbsp;Komal Bilal ,&nbsp;Samir Ibenmoussa ,&nbsp;Mohammed Bourhia ,&nbsp;Yousef A. Bin Jardan ,&nbsp;Muhammad Farooq ,&nbsp;Furhan Iqbal","doi":"10.1016/j.vprsr.2024.101069","DOIUrl":"https://doi.org/10.1016/j.vprsr.2024.101069","url":null,"abstract":"<div><p>Fort Munro is a hill station in Southern Punjab the residents of whom are heavily dependent upon livestock for their living but ticks are a big treat for the livestock. The purpose of this study was to ascertain the tick infestation among the small ruminants of Fort Munro. Ticks (<em>N</em> = 273) were collected from 333 animals (165 goats and 168 sheep) during August and September 2022. Two tick genera <em>Hyalomma and Rhipicephalus</em> were identified. <em>Rhipicephalus</em> was most abundant tick genera (78.02%) followed by <em>Hyalomma</em> (21.98%).Tick distribution significantly varied with the host: <em>Rhipicephalus</em> ticks were more frequently infesting goats while <em>Hyalomma</em> were more common on sheep. For both hosts, tick infestation varied with the sampling sites and male animals were significantly more infested than females. Five tick species were infesting goats with the following relative abundance: <em>Rhipicephalus</em> (<em>R.</em>) <em>senegalensis</em> (39.20%) &gt; <em>R. sanguineus</em> (36.8%) &gt; <em>R. appendiculatus</em> (10.40%) &gt; <em>R. turanicus</em> (8%) &gt; <em>R. guilhoni</em> (5.6%). <em>Rhipicephalus senegalensis</em> (41.22%) was also the most common tick species infesting sheep followed by <em>Hyalomma</em> (<em>H.</em>) <em>marginatum</em> (29.73%), <em>R. guilhoni</em> (18.24%), <em>H. dromedarii</em> (5.41%) and <em>H. impeltatum</em> (5.41%). Male ticks very more abundantly on both goats and sheep than female. Distribution of <em>Hyalomma</em> species also varied between the sampling sites. In conclusion, we are reporting the infestation of 3 <em>Hyalomma and</em> 5 <em>Rhipicephalus</em> in small ruminants of Fort Munro. The data generated through this study will help in developing appropriate tick control in the study area and will add to the existing knowledge regarding tick species that are infesting the small ruminants of Pakistan.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101069"},"PeriodicalIF":1.4,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of a point-of-care test for the detection of anti-Leishmania infantum antibodies is associated with immunofluorescent antibody titer and clinical stage of leishmaniosis in dogs from endemic regions","authors":"Kristen Davenport ,&nbsp;Joe Liu ,&nbsp;Juliana Sarquis ,&nbsp;Melissa Beall ,&nbsp;Ana Montoya ,&nbsp;Jan Drexel ,&nbsp;Tori Denis ,&nbsp;Ryan Toste ,&nbsp;Donato Traversa ,&nbsp;Guadalupe Miró","doi":"10.1016/j.vprsr.2024.101061","DOIUrl":"10.1016/j.vprsr.2024.101061","url":null,"abstract":"<div><p>Canine leishmaniosis (CanL) is caused by the protozoal parasite <em>Leishmania infantum</em>, which is transmitted by sand flies in warm climates across the world. Because dogs are considered a primary domestic reservoir for the parasite that causes leishmaniosis in humans, it is important from a One Health perspective that CanL be properly managed. In endemic regions, CanL is a common differential diagnosis in sick dogs because the clinical signs and clinicopathological disorders of the disease are non-specific, variable, and may overlap those of other common conditions. Diagnosis is based on the presence of compatible clinical signs, laboratory abnormalities, and confirmation by serological and parasitological evidence of infection. Here, we describe the performance of a point-of-care (POC) immunoassay that uses recombinant antigens to detect canine anti- <em>L. infantum</em> antibodies in a convenience sample set from a diagnostic laboratory, a group of canine patients with clinical staging, and in apparently healthy dogs from endemic areas. An immunofluorescence antibody test (IFAT) was used as the semiquantitative reference method. In the convenience sample set with high IFAT titers (≥ 1:800), the POC immunoassay demonstrated perfect agreement with IFAT (100%; 90/90). Using samples from dogs staged as either LeishVet Stage 2 or 3 or LeishVet Stage 1, positive agreement of the POC immunoassay with the IFAT was 98.8% (82/83) and 83.8% (31/37), respectively. The negative agreement with IFAT was 98.9% (272/275) in apparently healthy dogs from endemic areas of Greece and Italy. Since the performance of the POC immunoassay was associated with IFAT titer and clinical stage of CanL, the test may help veterinarians when determining if CanL is likely responsible for a patient's clinical picture or when evaluating an apparently healthy patient prior to vaccination.</p></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"53 ","pages":"Article 101061"},"PeriodicalIF":1.4,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405939024000819/pdfft?md5=0b65bceff140bb2506d66a9b44d165dc&pid=1-s2.0-S2405939024000819-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141394697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}