Molecular detection and lineage differentiation of Trypanosoma cruzi in dogs from a metropolitan area of Central México: Implications for urban zoonotic transmission

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-03-25 DOI:10.1016/j.vprsr.2025.101251

S. Zamora-Ledesma , N. Hernández-Camacho , M. Sánchez-Moreno , H. Ruiz-Piña , F.J. Escobedo-Ortegón , M.E. Villagrán-Herrera , C. Marín-Sánchez , B. Camacho-Macías

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Abstract

Trypanosoma cruzi is the etiological agent of Chagas disease, a zoonotic infection that affects millions of people across the Americas. Despite its well-documented presence in rural and wild environments, little is known about its transmission dynamics in rapidly urbanizing areas. This study aimed to investigate the prevalence and genetic diversity of T. cruzi in domestic dogs from the Metropolitan Area of Querétaro, Mexico, a region where no formal studies of Chagas disease transmission have been conducted. A total of 303 canine blood samples were analyzed using PCR, and 5.9 % tested positive for T. cruzi. Lineage-specific PCR revealed that both TcI and TcII strains were present, suggesting complex transmission cycles potentially involving both wild and domestic reservoirs. These findings underscore the need for further research on vector ecology and the role of urban wildlife in maintaining T. cruzi transmission, highlighting an emerging risk for urban populations.

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墨西哥中部大城市犬克氏锥虫的分子检测和谱系分化：城市人畜共患病传播的意义

克氏锥虫是恰加斯病的病原体，恰加斯病是一种影响美洲数百万人的人畜共患感染。尽管在农村和野生环境中有充分的记录，但对其在快速城市化地区的传播动态知之甚少。本研究的目的是调查来自墨西哥quersamaro市区的家狗中克氏t型虫的流行情况和遗传多样性，该地区尚未开展恰加斯病传播的正式研究。用聚合酶链反应（PCR）对303份犬血样本进行了分析，5.9%的犬血样本呈克鲁兹绦虫阳性。谱系特异性PCR显示TcI和TcII菌株同时存在，表明复杂的传播周期可能涉及野生和国内水库。这些发现强调需要进一步研究媒介生态学和城市野生动物在维持克氏体传播中的作用，并强调城市人群面临的新风险。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).