Molecular identification of bald eagle (Haliaeetus leucocephalus) from Alaska, USA as a natural definitive host for Sarcocystis infections of bovid and cervid intermediate hosts

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-04-07 DOI:10.1016/j.vprsr.2025.101259

Aditya Gupta , Kimberlee Beckmen , Benjamin M. Rosenthal , Jitender P. Dubey

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Abstract

Sarcocystis sporocysts were found in intestinal scrapings of two Bald eagles (Haliaeetus leucocephalus) from Alaska, USA. The sporocysts/oocysts were characterized molecularly using Sarcocystis-specific primers targeting the 18S rRNA, 28S rRNA, cox1, and a region spanning 18S rRNA, ITS1, and 5.8S rRNA genes. The PCR amplification confirmed the presence of three Sarcocystis species in the bald eagles. They are: either of Sarcocystis bovini-like or Sarcocystis bovifelis-like parasites of European cattle (Bos taurus), Sarcocystis sp. of elk (Cervus canadensis), and either of Sarcocystis tarandi-like of deer (Rangifer tarandus) or Sarcocystis elongata-like parasites of red deer (Cervus elaphus) with identities of >98 %. This is the first report of H. leucocephalus as a definitive host for cervid and bovid infections. It is also the first report of S. bovini-like / S. bovifelis-like and S. tarandi-like / S. elongata-like parasites in the USA. Epidemiologic significance of findings is discussed.

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美国阿拉斯加州秃鹰（halaliaeetus leucocephalus）作为牛科和子科中间宿主肉囊虫感染天然终宿主的分子鉴定

在美国阿拉斯加州的两只白头海雕（halaliaeetus leucocephalus）的肠屑中发现了肉囊菌孢子囊。利用针对18S rRNA、28S rRNA、cox1和一个跨越18S rRNA、ITS1和5.8S rRNA基因的区域的肌囊虫特异性引物对孢子囊/卵囊进行分子表征。PCR扩增证实了白头海雕中存在三种肉囊菌。它们是：欧洲牛（Bos taurus）的类牛肉囊虫或类牛肉囊虫寄生虫，麋鹿（Cervus canadensis）的类鹿肉囊虫，以及鹿（Rangifer tarandus）的类鹿肉囊虫或马鹿（Cervus elaphus）的类长肉囊虫寄生虫，其鉴定率为98%。这是首次报道白头绦虫作为宫颈和牛科感染的最终宿主。这也是美国首次报道牛链球菌样/牛链球菌样和塔兰蒂链球菌样/长形链球菌样寄生虫。讨论了研究结果的流行病学意义。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).