First report of natural Besnoitia akodoni infection in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-03-24 DOI:10.1016/j.vprsr.2025.101245

Judith V. Bentancourt Rossoli , Gastón Moré , Agustina Soto-Cabrera , Dadín P. Moore , Eleonora L. Morrell , Lucía M. Campero , Walter Basso , Yanina P. Hecker , Nathalia P. Scioscia

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Abstract

Besnoitia spp. are cyst-forming Apicomplexa parasites within the Sarcocystidae family. The objective of this study was to identify Besnoitia spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Samples were collected from different organs and tissues of 356 rodents and examined by histopathological methods. Besnoitia cysts were detected by histopathology in 2 % (7/356) of the animals (i.e., two Mus musculus, two Necromys spp., two Oxymycterus rufus, and one Akodon azarae). The morphological diagnosis was molecularly confirmed by PCR and bidirectional sequencing of the ITS1 marker and flanking regions. The complete ITS1 region was identical in all seven samples, exhibiting 100 % identity with Besnoitia akodoni (AY545987). In addition, further characterization of the isolates targeting 18S rRNA and CoxI gene fragments was performed. Five identical 18S rRNA consensus sequences were amplified, which matched 100 % with Besnoitia sp. from Abrothrix hirta (Chile; OR052141) and B. jellisoni (AF291426) sequences. Besides, three CoxI consensus sequences were obtained from three different rodent species. These were identical among them and showed 92.88–92.93 % similarity to sequences from Besnoitia besnoiti uncharacterized protein (XM_029362743, among others). This is the first study to identify B. akodoni in naturally infected murid (Mus musculus) and cricetid (Necromys spp., Oxymycterus rufus, and A. azarae) rodents from Argentina, using both histopathological and molecular assessment. Further research is necessary to elucidate the significance of this parasite in rural ecosystems in our country.

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阿根廷合群（鼠科）和野生（鼠科）啮齿动物自然感染阿氏贝斯诺蒂亚虫的首次报告

麻囊虫是囊虫科中形成囊状的顶复合体寄生虫。本研究的目的是鉴定阿根廷合种（鼠科）和野生（鼠科）啮齿动物中的Besnoitia属。从356只啮齿动物的不同器官和组织中采集样本，采用组织病理学方法进行检测。组织病理学检查发现2%（7/356）的动物（2只小家鼠、2只死灵鼠、2只鲁夫斯oxymyterus和1只阿扎雷Akodon）存在脓肿囊肿。形态学诊断通过PCR和ITS1标记和侧翼区域的双向测序得到分子证实。完整的ITS1区域在所有7个样本中都是相同的，与Besnoitia akodoni （AY545987）具有100%的一致性。此外，我们还对靶向18S rRNA和CoxI基因片段的分离株进行了进一步的鉴定。扩增出5个相同的18S rRNA序列，与智利Abrothrix hirta （Chile）的Besnoitia sp.匹配100%；OR052141)和B. jellisoni （AF291426）序列。此外，从3种不同的鼠类中获得了3个CoxI一致序列。结果表明，这些序列的相似性为92.88 ~ 92.93%，与未鉴定蛋白（XM_029362743等）相似。这是首次在阿根廷自然感染的鼠（小家鼠）和cricetid （Necromys spp.， Oxymycterus rufus和A. azarae）啮齿动物中鉴定akodoni的研究，采用了组织病理学和分子评估。该寄生虫在我国农村生态系统中的意义有待进一步研究。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).