Transboundary and Emerging Diseases最新文献

{"title":"A Single-Copy Sensitive and Field-Deployable One-Pot RT-RPA CRISPR/Cas12a Assay for the Specific Visual Detection of the Nipah Virus","authors":"Kaikai Jin,&nbsp;Pei Huang,&nbsp;Boyi Li,&nbsp;Zengguo Cao,&nbsp;Zanheng Huang,&nbsp;Zimo Zhang,&nbsp;Meihui Liu,&nbsp;Hao Li,&nbsp;Lijuan Niu,&nbsp;Tianyi Zhang,&nbsp;Yuanyuan Li,&nbsp;Xuemeng Li,&nbsp;Hualei Wang,&nbsp;Haili Zhang","doi":"10.1155/2024/4118007","DOIUrl":"https://doi.org/10.1155/2024/4118007","url":null,"abstract":"<div>\u0000 <p>Nipah virus (NiV) is an emerging bat-borne zoonotic virus that can be transmitted to humans and other animals through infected bats or contaminated foods. The disease is highly lethal in humans (40%–75%) and has the potential for human-to-human transmission. Currently, there are no approved treatments or vaccines for NiV infection in humans or animals. Consequently, there is a pressing need for a highly sensitive, precise, and visually detectable assay to enable early intervention and mitigate the transmission of NiV infection. Here, we report a single-copy sensitive, field-deployable, one-pot visual reverse transcription-recombinase polymerase amplification (RT-RPA)-clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associate system (Cas)12 for the detection of NiV. The assay works by targeting the <i>N</i> gene of NiV, and the results are directly visible to the naked eye. The assay has demonstrated the ability to detect as few as 5.5 copies/μl of positive plasmids or 5.5 × 10¹ copies/μl of RNA transcripts when reacted at constant temperature for 40 min. It showed high specificity for NiV and had no cross-reaction with other pathogens, including rabies virus (RABV), Japanese encephalitis virus (JEV), herpes simplex virus type 1 (HSV-1), Hendra virus (HeV), and <i>Streptococcus suis</i> (<i>S. suis</i>), that can cause clinical symptoms similar to those of NiV infection. Moreover, this assay had a 100% coincidence rate with the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method recommended by the World Organization for Animal Health (WOAH) for the detection of simulated clinical samples, indicating that it has great potential as an ultrasensitive, simple, and portable novel assay for the onsite diagnosis of NiV infection.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4118007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Global Distribution and Characteristics of Research Studies Focusing on Swine Farm Biosecurity: A Scoping Review","authors":"Isha Agrawal,&nbsp;Erin E. Kerby,&nbsp;Csaba Varga","doi":"10.1155/2024/6497633","DOIUrl":"https://doi.org/10.1155/2024/6497633","url":null,"abstract":"<div>\u0000 <p>Despite significant advances in swine biosecurity (BS) over the last decade, BS plans have yet to be broadly adopted on swine farms. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScRs) framework was followed to review the literature, describe the worldwide distribution of publications on swine farm BS, and characterize the research methodologies used. The final data extraction and analysis included 157 publications originating from 48 countries. Several publications (<i>n</i> = 93) used face-to-face interviews for data collection. An increase in the adoption of online and multimode approaches was detected after 2009. Many publications (<i>n</i> = 92) focussed on the impact of BS on the incidence of swine diseases such as porcine reproductive and respiratory syndrome (PRRS) and African swine fever (ASF). Only 16 studies reported proposing incentives for study participation. Regions with high publication numbers were detected in Western and Southern Europe, Northeast of South America, and East Africa. Areas with low publication numbers were in Eastern Europe, North and Central Africa, Central America, and the Northwest of South America. This study identified the most common study methodologies used to assess swine farm BS. Countries with limited swine BS research studies were identified where future investigations are needed.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6497633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of HPAI H5N6 Clade 2.3.4.4b in Wild Birds: A Case Study From South Korea, 2023","authors":"Chang-Gi Jeong,&nbsp;Chung-Young Lee,&nbsp;Su-Beom Chae,&nbsp;Jung-Hoon Kwon,&nbsp;Eun-Jee Na,&nbsp;Jun-Soo Park,&nbsp;Young-Sik Kim,&nbsp;Seung-Chai Kim,&nbsp;Hwan-Ju Kim,&nbsp;Young-Sun Sung,&nbsp;Sun-Young Kim,&nbsp;Won-Il Kim,&nbsp;Jae-Ku Oem","doi":"10.1155/tbed/4141478","DOIUrl":"https://doi.org/10.1155/tbed/4141478","url":null,"abstract":"<div>\u0000 <p>The emergence and evolution of avian influenza A viruses (AIVs) pose significant challenges to both public health and animal husbandry worldwide. Here, we characterized a novel reassortant highly pathogenic avian influenza virus (HPAIV), clade 2.3.4.4b H5N6, that was isolated from a mandarin duck in South Korea in December 2023. Phylogenetic and molecular analyses show that the hemagglutinin (HA) gene of the 23-JBN-F12-36/H5N6 virus clustered with HPAIV clade 2.3.4.4b H5N1 viruses, which were circulating in South Korea and Japan in 2022–2023. The M and polymerase acidic (PA) genes also revealed a close association with the HPAIV clade 2.3.4.4b H5N1 AIV that was identified previously in South Korea during November 2022. Notably, the neuraminidase (NA) gene of the 23-JBN-F12-36/H5N6 virus was estimated to have its origins in the HPAIV clade 2.3.4.4h H5N6 prevalent in poultry in China, and it is clustered with the AIVs that are associated with human infection cases. Taken together, these results show that the virus has been produced by reassortment with H5N1 HPAIV, which is prevalent in wild birds; H5N6 HPAIV, which is circulated in poultry in China; and the internal genes of low pathogenic avian influenza viruses (LPAIVs). In light of the reassortment of HPAIVs circulating in existing wild birds and HPAIVs circulating in poultry in China within the 2.3.4.4b H5Nx clade, it is imperative to strengthen active surveillance across wild bird populations, poultry farms, and live poultry markets, and to inform for the effective design of improved prevention and control strategies.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4141478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Syndemic Challenges: Addressing the Resurgence of Mpox Amidst Concurrent Outbreaks in the DRC","authors":"Olaniyi Abideen Adigun,&nbsp;Olalekan John Okesanya,&nbsp;Mohamed Mustaf Ahmed,&nbsp;Bonaventure Michael Ukoaka,&nbsp;Don Eliseo Lucero-Prisno III,&nbsp;Emmanuella Ogechi Onyeaghala,&nbsp;Emmanuel Ayomide Oluwasusi,&nbsp;Olamide Esther Ogunwale,&nbsp;Ayodeji Amos Faniyi","doi":"10.1155/tbed/1962224","DOIUrl":"https://doi.org/10.1155/tbed/1962224","url":null,"abstract":"<div>\u0000 <p>The Democratic Republic of Congo (DRC) faces a syndemic of infectious diseases, including monkeypox (mpox), cholera, measles, anthrax, and plague, worsening public health challenges and socioeconomic disparities. This review synthesizes and discusses epidemiological data and consequences of simultaneous outbreaks in the DRC between January 2023 and March 2024. The findings highlight a 6.7% fatality rate and 3319 confirmed cases of mpox, with significant outbreaks in Kinshasa and 22 other provinces. Anthrax occasionally surfaced among cattle-raising villages, measles affected fewer than five children susceptible to the disease, and cholera outbreaks persisted in North Kivu, South Kivu, and Tanganyika. Plague incidences, mostly bubonic, have been reported in Ituri province. Vulnerable groups, including children, mothers, the elderly, and those with compromised immune systems, face increased risks due to poor healthcare access, hunger, and underlying medical conditions. Cultural beliefs, healthcare system issues, and socioeconomic instability impede effective response tactics. This strain on the fragile healthcare system highlights the need for increased surveillance, immunization efforts, and community involvement. To mitigate the effects of syndemic outbreaks, strengthening the DRC’s health systems through international cooperation, integrated public health initiatives, and improved access to healthcare is crucial.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/1962224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specific Detection of RHDV GI.1 and GI.2 by RT-LAMP-CRISPR/Cas12a Platform","authors":"Mengting Wu,&nbsp;Mengmeng Chen,&nbsp;Rulong Qiu,&nbsp;Lei Ge,&nbsp;Zhiyu Fan,&nbsp;Bo Hu,&nbsp;Houjun Wei,&nbsp;Yiming Li,&nbsp;Fang Wang,&nbsp;Yanhua Song","doi":"10.1155/tbed/3881457","DOIUrl":"https://doi.org/10.1155/tbed/3881457","url":null,"abstract":"<div>\u0000 <p>Rabbit hemorrhagic disease is a highly contagious and acute fatal disease caused by rabbit hemorrhagic disease virus (RHDV). The first outbreak of RHDV2 in 2020 has posed a serious threat to the rabbit breeding industry in China. An effective and specific detection strategy for RHDV GI.1 (RHDV1) and GI.2 (RHDV2) is urgently needed. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP)-CRISPR/Cas12a-based dual readout portable detection platform. The platform showed excellent specificity to identify RHDV1 and RHDV2 strains and no cross-reaction with other prevalent pathogens of rabbit. The detection limit for RHDV1 and RHDV2 by RT-LAMP-CRISPR/Cas12a could reach 10 copies/μl of the VP60 gene per reaction. Furthermore, 74 clinical samples were detected for both RHDV1 and RHDV2. RT-LAMP-CRISPR/Cas12a-based dual readout portable detection platform showed 25.68% (19/74) RHDV1-positive samples, 43.24% (32/74) RHDV2-positive samples, and 8.11% (6/74) RHDV1/RHDV2 double positive samples, respectively. The coincidence rates of detection RHDV1 and RHDV2 between RT-LAMP-CRISPR/Cas12a and quantitative real-time-polymerase chain reaction (qPCR) were both 97.30%. RT-LAMP-CRISPR/Cas12a showed higher sensitivity and detection rate compared with qPCR. Moreover, the results were visible to the naked eye within 1.5 h combined with lateral flow strips (LFSs) and visual fluorescence. The RT-LAMP-CRISPR/Cas12a portable platform has the advantages of high sensitivity, specificity, fast, low equipment requirements, which can be used in clinical practice in rural areas and resource-limited settings.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/3881457","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Prevalence Estimation and Phylogenetic Analysis of Novel Porcine Parvoviruses (PPV2–7) in Hungarian Pig Herds","authors":"Barbara Igriczi,&nbsp;Lilla Dénes,&nbsp;Kitti Schönhardt,&nbsp;Aleksandra Woźniak,&nbsp;Tomasz Stadejek,&nbsp;Gyula Balka","doi":"10.1155/2024/5117884","DOIUrl":"https://doi.org/10.1155/2024/5117884","url":null,"abstract":"<div>\u0000 <p>To date, seven novel parvoviruses have been identified in pigs and designated as porcine parvovirus 2–7 (PPV2–7). The presence of these emerging viruses has been reported in several countries around the world, although their pathogenic role and clinical and economical relevance are largely unknown. Here, we report the estimated prevalence and genetic diversity of novel PPV2–7 in Hungarian pig herds and the detection of these viruses in two Slovakian pig farms. For the comparative prevalence estimation, 2505 serum samples from different age groups, 218 oral fluid samples, and 111 processing fluid samples were collected from 26 large-scale Hungarian farms according to a systematic, cross-sectional sampling protocol. All samples were tested by real-time quantitative polymerase chain reaction (qPCR), and the presence of at least one PPV was detected in 24 of the 26 (92%) Hungarian and both Slovakian farms, suggesting high levels of subclinical circulation in most herds. The estimated PPV2–7 prevalence in Hungary varied from 50% to 89%, with PPV4 being the least and PPV2 being the most prevalent virus. The highest detection rates were observed in oral fluid samples, indicating that this sample type is most suitable for screening PPVs, but all viruses were also detected in serum samples and processing fluids. All novel PPVs were most frequently detected in the serum samples of weaned pigs and fatteners, with slightly higher viral burden in the younger age groups. These results may suggest an age-related susceptibility, which could play a significant role in the epidemiology of these viruses, impacting herd health and productivity.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5117884","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methods to Estimate the Between-Population Level Effective Reproductive Number for Infectious Disease Epidemics: Foot-And-Mouth Disease (FMD) in Vietnam","authors":"Umanga Gunasekera,&nbsp;Kimberly VanderWaal,&nbsp;Jonathan Arzt,&nbsp;Andres Perez","doi":"10.1155/2024/4114217","DOIUrl":"https://doi.org/10.1155/2024/4114217","url":null,"abstract":"<div>\u0000 <p>Foot-and-mouth disease (FMD), which is endemic in 77% of countries globally, is a major threat to the global livestock industry. Knowledge of the reproductive number at the population level (i.e., farm level, herd level, or above) for FMD is important to estimate the magnitude of epidemics and design and implement effective control methods. Different methods, based on disparate assumptions and limitations, have been used interchangeably to compute and report reproductive numbers at the population level without a formal comparison between them. This study compares the results obtained when using alternative methods to compute between populations (<i>R</i>_bp) for FMD using one single dataset collected over 10 years (2007–2017) at the commune-level swine farms in Vietnam. Seven spatial–temporal clusters were identified in the country, and the value of <i>R</i>_bp was computed on each of them using different analytical approaches, namely, epidemic doubling time, nearest neighbor, time-dependent reproductive number (TDR), sequential Bayesian (SB), and birth–death skyline (BDSKY) analysis in Bayesian evolutionary analysis by sampling trees 2 (BEAST2). Estimated <i>R</i>_bp values were relatively similar across methods ranging from 1.25 to 1.61. For the first time, the results here provide a comparison of different methods used to compute <i>R</i>_bp for FMD. Despite differences in assumptions and limitations, results suggest that different methods produce relatively similar outputs. Additionally, the results here provide foundational knowledge to support the evaluation and control of FMD epidemics in a population.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4114217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Systematic Review and Meta-Analysis of the Efficacy of Biosecurity in Disease Prevention and Control in Livestock Farms in Africa","authors":"Ronald Vougat Ngom,&nbsp;Gaspard J. Ayissi,&nbsp;Adonis M. M. Akoussa,&nbsp;Andrea Laconi,&nbsp;Saleh M. Jajere,&nbsp;Henriette A. Zangue,&nbsp;Alessandra Piccirillo","doi":"10.1155/2024/8683715","DOIUrl":"https://doi.org/10.1155/2024/8683715","url":null,"abstract":"<div>\u0000 <p>In Africa, livestock production plays a crucial role for sustainable food security and economic growth. However, the development of this sector has been delayed by livestock diseases, one of the main constraints, which can cause important production and economic losses. To overcome these constraints, farmers extensively use antimicrobials, which in turn can lead to antimicrobial resistance (AMR), one of the main threats to global health and food security. Biosecurity has been identified as a key strategy to reduce livestock diseases. Therefore, the current systematic review and meta-analysis, conducted according to the Cochrane guideline, aimed at determining the efficacy of biosecurity in preventing and controlling infectious diseases in livestock farms in Africa. Of the 1408 records retrieved from five different databases, only 16 met the inclusion criteria. These studies were conducted in Egypt (31.2%), Nigeria (31.2%), Uganda (18.8%), Ethiopia (12.5%) and Tunisia (6.3%) and concerned poultry (62.4%), pigs (18.8%) and cattle (18.8%). Investigations focused mainly on avian influenza (AI) (15.0%) and coccidiosis (10.0%) in poultry and African swine fever (ASF) (10.0%) in pigs. In poultry farms, the results of the pairwise meta-analysis showed that biosecurity measures related to visitors and farmworkers could be effective at reducing the risk of introduction and spread of AI viruses (odds ratio [OR] = 0.48; 95% confidence interval [CI] 0.28–0.82). Moreover, inadequate biosecurity seemed to be a factor promoting coccidiosis (OR = 4.20; 95% CI 2.4–7.4) and AI (OR = 1.74; 95% CI 1.23–2.48). Prevention of ASF was significantly associated with the application of biosecurity measures related to animals’ transport, removal of carcasses and manure (OR = 0.33; 95% CI 0.12–0.88). Despite their importance, these findings cannot be translated to the entire African continent, since no studies were available for more than 90% of its countries. More research should be carried out to fill in the gaps identified by this review.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8683715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Genomic Characteristics of a Novel Pathogenicity Type I Feline Coronavirus in Mainland China","authors":"Yuanhong Wang,&nbsp;Junna Wang,&nbsp;You Zhao,&nbsp;Yun Liu,&nbsp;Miao Zhang,&nbsp;Xiaoying Deng,&nbsp;Jie Zhu,&nbsp;Guoxin Li,&nbsp;Guangqing Liu","doi":"10.1155/2024/4162458","DOIUrl":"https://doi.org/10.1155/2024/4162458","url":null,"abstract":"<div>\u0000 <p>Feline coronavirus (FCoV) is an enveloped, positive-sense RNA virus, which is widespread among feline populations, and can cause a fatal serious disease called feline infectious peritonitis (FIP). According to the differences of antigen and genetic composition, FCoV consists of two genotypes, FCoV I and FCoV II. In this study, we have isolated and identified a FCoV I strain named HL2019. Based on the complete genome of HL2019, phylogenetic analysis showed that HL2019 strain formed in the cluster FCoV I which is more closed to human coronavirus 229E (HCoV 229E) and HCoV NL63, while the FCoV I stains is distantly related to FCoV II strains. Analyzing with RDP4 and Simplot software showed that the virus HL2019 is recombinant by the FCoV I China/ZJU1709 and FCoV I Netherlands/UU16 strains. Furthermore, the pathogenicity of HL2019 was evaluated in 9–12-month-old cats. Two of three challenged cats developed serious clinical signs and died at 28-day postchallenge (dpc). Real-time polymerase chain reaction (PCR) analysis showed that HL2019 has broad tissue tropism, especially in the duodenum with viral load up to 10⁴ copies/mg. In summary, our data show that we have successfully isolated a strain of FCoV I named HL2019 that is highly pathogenic to cats.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4162458","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and In Vivo Characterization of the High Pathogenicity H7N6 Avian Influenza Virus That Emerged in South African Poultry in 2023","authors":"Celia Abolnik,&nbsp;Thandeka Precious Phiri,&nbsp;Christine Strydom,&nbsp;Zehaad Ismail,&nbsp;Frances Jordaan,&nbsp;Kaila Wannenburg,&nbsp;Shahn P. R. Bisschop","doi":"10.1155/2024/8878789","DOIUrl":"https://doi.org/10.1155/2024/8878789","url":null,"abstract":"<div>\u0000 <p>A high pathogenicity avian influenza (HPAI) subtype H7N6 virus emerged in South African poultry in 2023 and later spread to Mozambique, the first documented emergence of H7 HPAI in the African continent. A total of 6.82 million birds succumbed to the disease or were culled, representing about 20% of the South African egg-laying flock and almost 30% of the broiler breeder flock. The complete genomes of 68 outbreak viruses were sequenced and analyzed, tracing the phylogenetic origins of the ancestral H7N6 virus to a reassortment of various subtypes that circulated in southern African wild birds. Molecular clock analysis determined that the virus emerged in the first week of May 2023, probably in a smallholder chicken flock, before spreading to commercial farms, where the disease was first reported in early June. The multibasic hemagglutinin protein cleavage site (HA₀) was derived from a nonhomologous recombination event with chicken 28S ribosomal ribonucleic acid (RNA). Few genetic markers associated with an increased risk to humans were present in the translated viral proteins. The intravenous pathogenicity index (IVPI) value of the index case isolate was 1.67, reflecting that 50% of the specific pathogen-free chickens died within 4 days of infection. Surviving birds showing mostly mild clinical signs and recovered by day 10 postinfection. Prior to death, chickens shed the virus primarily through the respiratory route, with lower amounts shed from the cloaca, but in the survivors, the virus was still being shed from the cloaca on day 10. Fomites were the likely source of disease spread between farms, and the amount of H7N6 HPAI virus per gram of feces was calculated at ~383,193 (5.58 log₁₀) egg infectious dose 50 (EID₅₀) equivalents, chicken feather follicles contained on average 739,712.43 (5.87 log₁₀) EID₅₀ equivalents, and 20 µg of feather dust contained 14,976.96 (4.175 log₁₀) EID₅₀ equivalents.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8878789","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}