Transboundary and Emerging Diseases最新文献

{"title":"Detection of Tick-Borne Pathogen Coinfections and Coexposures to Foot-and-Mouth Disease, Brucellosis, and Q Fever in Selected Wildlife From Kruger National Park, South Africa, and Etosha National Park, Namibia","authors":"Carlo Andrea Cossu,&nbsp;Sunday Ochonu Ochai,&nbsp;Milana Troskie,&nbsp;Axel Hartmann,&nbsp;Jacques Godfroid,&nbsp;Lin-Mari de Klerk,&nbsp;Wendy Turner,&nbsp;Pauline Kamath,&nbsp;Ockert Louis van Schalkwyk,&nbsp;Rudi Cassini,&nbsp;Raksha Bhoora,&nbsp;Henriette van Heerden","doi":"10.1155/tbed/2417717","DOIUrl":"https://doi.org/10.1155/tbed/2417717","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Although the rate of emerging infectious diseases that originate in wildlife has been increasing globally in recent decades, there is currently a lack of epidemiological data from wild animals.</p>\u0000 <p><b>Methodology:</b> We used serology to determine prior exposure to foot-and-mouth disease virus (FMDV), <i>Brucella</i> spp., and <i>Coxiella burnetii</i> and used genetic testing to detect blood-borne parasitic infections in the genera <i>Ehrlichia</i>, <i>Anaplasma</i>, <i>Theileria</i>, and <i>Babesia</i> from wildlife in two national parks, Kruger National Park (KNP), South Africa, and Etosha National Park (ENP), Namibia. Serum and whole blood samples were obtained from free-roaming plains zebra (<i>Equus quagga</i>), greater kudu (<i>Tragelaphus strepsiceros</i>), impala (<i>Aepyceros melampus</i>), and blue wildebeest (<i>Connochaetes taurinus</i>). Risk factors (host species, sex, and sampling park) for infection with each pathogen were assessed, as well as the prevalence and distribution of co-occurring infections.</p>\u0000 <p><b>Results:</b> In KNP 13/29 (45%; confidence interval [CI]: 26%–64%) kudus tested positive for FMD, but none of these reacted to SAT serotypes. For brucellosis, seropositive results were obtained for 3/29 (10%; CI: 2%–27%) kudu samples. Antibodies against <i>C. burnetii</i> were detected in 6/29 (21%; CI: 8%–40%) kudus, 14/21 (67%; CI: 43%–85%) impalas, and 18/39 (46%; CI: 30%–63%) zebras. A total of 28/28 kudus tested positive for <i>Theileria</i> spp. (100%; CI: 88%–100%) and 27/28 for <i>Anaplasma/Ehrlichia</i> spp. (96%; CI: 82%–100%), whereas 12/19 impalas (63%) and 2/39 zebra (5%) tested positive for <i>Anaplasma centrale</i>. In ENP, only 1/29 (3%; CI: 0%–18%) wildebeest samples tested positive for FMD. None of the samples tested positive for brucellosis, while <i>C. burnetii</i> antibodies were detected in 26/30 wildebeests (87%; CI: 69%–96%), 16/40 kudus (40%; CI: 25%–57%), and 26/26 plains zebras (100%; CI: 87%–100%). A total of 60% <i>Anaplasma/Ehrlichia</i> spp. and 35% <i>Theileria/Babesia</i> spp. in kudu and 37% wildebeest tested positive to <i>Theileria</i> sp. (sable), 30% to <i>Babesia occultans</i>, and 3%–7% to <i>Anaplasma</i> spp. The seroprevalence of Q fever was significantly higher in ENP, while <i>Brucella</i> spp., <i>Anaplasma</i>, <i>Ehrlichia</i>, <i>Theileria</i>, and <i>Babesia</i> species were significantly higher in KNP. Significant coinfections were also identified.</p>\u0000 <p><b>Conclusion:</b> This work provided baseline serological and molecular data on 40+ pathogens in four wildlife species from two national parks in southern Africa.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/2417717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142860835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"West Nile Virus Monitoring in Flanders (Belgium) During 2022–2023 Reveals Endemic Usutu Virus Circulation in Birds","authors":"C. Sohier,&nbsp;F. C. Breman,&nbsp;M. Vervaeke,&nbsp;N. De Regge","doi":"10.1155/tbed/4146156","DOIUrl":"https://doi.org/10.1155/tbed/4146156","url":null,"abstract":"<div>\u0000 <p>The recent emergence of West Nile virus (WNV) and Usutu virus (USUV) in some European countries has triggered an increase in animal and human cases across Europe. Wild birds, serving as key reservoirs for WNV and USUV, often act as crucial indicators for the introduction and spread of these viruses. Currently, there is no durable large-scale monitoring for WNV in Belgium, and specific monitoring for USUV is lacking. In Flanders, passive WNV monitoring in birds has been in place for many years, while initial efforts to initiate active monitoring started in 2022. Here, we present the results of a limited study conducted during the vector seasons of 2022 and 2023 in Flemish bird populations to actively and passively monitor the prevalence of WNV and additionally assess the presence of USUV. Several real-time reverse transcription-PCR tests were employed for virus detection, revealing the absence of WNV-RNA during both vector seasons. Conversely, USUV-RNA was identified in 2022 through active surveillance, affecting two (5.5%) out of 36 birds (<i>Corvus corone</i>), and in passive surveillance, impacting eight (72.7%) out of 11 birds (<i>Turdus merula</i> [6] and <i>Rhea pennata</i> [2]). In 2023, active surveillance was more extensive and identified 16 (7.2%) USUV-RNA positive birds (<i>Buteo buteo</i> [1], <i>T. merula</i> [14] and <i>Athene noctua</i> [1]) out of 222 examined birds, while passive surveillance detected two (7.1%) positive birds (<i>T. merula</i> [1], and <i>Larus marinus</i> [1]) out of 28. Viral sequence information was obtained from seven USUV-positive birds using whole genome sequencing or Sanger sequencing. Phylogenetic analysis placed all identified strains within the Africa 3 lineage. This restricted WVN monitoring effort in Flanders did not reveal WNV presence, but found indications of an endemic USUV circulation in Belgium. It is crucial to intensify monitoring efforts for WNV in the coming years, considering its endemic status in several European countries and its expanding geographical range in northern Europe.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4146156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142860865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal Active Avian Influenza Surveillance in Bangladesh From 2017–2022 Reveals Differential IAV and H5 Infection and Viral Burden Associated With Bird Species, Sex, and Age","authors":"Walter N. Harrington,&nbsp;Jasmine C. M. Turner,&nbsp;Subrata Barman,&nbsp;Mohammed M. Feeroz,&nbsp;Md. Kamrul Hasan,&nbsp;Sharmin Akhtar,&nbsp;Trushar Jeevan,&nbsp;Nabanita Mukherjee,&nbsp;Patrick Seiler,&nbsp;John Franks,&nbsp;David Walker,&nbsp;Pamela McKenzie,&nbsp;Lisa Kercher,&nbsp;Robert G. Webster,&nbsp;Richard J. Webby","doi":"10.1155/tbed/5569836","DOIUrl":"https://doi.org/10.1155/tbed/5569836","url":null,"abstract":"<div>\u0000 <p>Influenza viruses are a major global health burden with up to 650,000 associated deaths annually. Beyond seasonal illness, influenza A viruses (IAVs) pose a constant pandemic threat due to novel emergent viruses that have evolved the ability to jump from their natural avian hosts to humans. Because of this threat, active surveillance of circulating IAV strains in wild and domestic bird populations is vital to our pandemic preparedness and response strategies. Here, we report on IAV surveillance data collected from 2017 to 2022 from wild and domestic birds in Bangladesh. We note evidence to suggest that male birds show a higher risk of IAV, including highly pathogenic avian influenza (HPAI) A(H5) virus, positivity than female birds. The data was stratified to control for selection bias and confounding variables to test the hypothesis that male birds are at a higher risk of IAV positivity relative to female birds. The association of IAV and A(H5) largely held in each stratum, and double stratification suggested that the phenomena was largely specific to ducks. Finally, we show that chickens, male birds, and juvenile birds generally have higher viral loads compared to their counterparts. These observations warrant further validation through active surveillance across various populations. Such efforts could significantly contribute to the enhancement of pandemic prediction and risk assessment models.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/5569836","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142860834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A RFLP 1-4-3 L1C Variant of PRRSV-2 Isolated in Sichuan Province, China: Genetic Characterization and Pathogenicity","authors":"Li-Shuang Deng,&nbsp;Zhi-Jie Jian,&nbsp;Yuan-Meng Wang,&nbsp;Bing-Zhou Huang,&nbsp;Tong Xu,&nbsp;Feng-Qin Li,&nbsp;Si-Yuan Lai,&nbsp;Yan-Ru Ai,&nbsp;Jian-Bo Huang,&nbsp;Zhi-Wen Xu,&nbsp;Ling Zhu","doi":"10.1155/tbed/6817783","DOIUrl":"https://doi.org/10.1155/tbed/6817783","url":null,"abstract":"<div>\u0000 <p>Porcine reproductive and respiratory syndrome virus (PRRSV), known for causing reproductive disorders in sows and respiratory issues in piglets, poses a significant threat to the global swine industry. Since its initial report in 2013, the L1C (lineage 1.8/NADC30-like) PRRSV has drawn significant attention in China due to its high recombination potential and diverse pathogenicity. This study focuses on a naturally occurring recombinant L1C variant, SCABTC-202302, characterized by an restriction fragment length polymorphism (RFLP) pattern of 1-4-3. We investigate the strain’s genetic evolution, recombination, pathogenicity, and immune and antibody responses. Phylogenetic analysis of the ORF5 (open reading frame) gene classified the SCABTC-202302 strain as lineage 8.7, while whole-genome analysis categorized it as L1C. Notably, a discontinuous deletion of 131 amino acids (AAs) was observed in the NSP2 gene, along with specific AA mutations in ORF5. Recombination analysis revealed the NADC30 strain as the primary parent, with contributions from the JXA1 strain in the ORF2-ORF7 region. The strain caused lung and lymph node damage, sustained high-level viremia, and elevated inflammatory factors in infected piglets. Our study provides valuable insights into the genetic characteristics, pathogenicity, and immunological profile of L1C strains, contributing to the development of vaccines and control measures for PRRSV.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/6817783","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142860864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to Crimean-Congo Hemorrhagic Fever Virus in Wild Ungulates in the Basque Country, Northern Iberian Peninsula","authors":"Aitor Cevidanes,&nbsp;Jesús F. Barandika,&nbsp;Gorka Aduriz,&nbsp;Ana Hurtado,&nbsp;Ana L. García-Pérez,&nbsp;Marta Barral","doi":"10.1155/tbed/8553577","DOIUrl":"https://doi.org/10.1155/tbed/8553577","url":null,"abstract":"<div>\u0000 <p>Crimean-Congo hemorrhagic fever virus (CCHFV) causes a serious human tick-borne disease. In animals, CCHFV infections are mainly subclinical. The circulation of the virus has received little attention in areas where the main vector (<i>Hyalomma</i> spp.) is not considered to be present or established (e.g., the Northern Iberian Peninsula). The presence of antibodies against CCHFV was evaluated in sera collected from 1190 wild boars, 36 red deer, and 36 roe deer in the Basque Country (Northern Iberian Peninsula) in 2014–2019. Antibodies were found in the three wild ungulate species with an overall prevalence of 2.5%. The highest seroprevalence was found in red deer (22.2%) and in the southwest province: Araba (8.6%). The presence of antibodies against CCHFV in wild ungulates reported in this study could be due to an underestimated presence of <i>Hyalomma</i> ticks, the sporadic exposure to infected <i>Hyalomma</i> ticks transported by animals (e.g., migratory birds), or the role of other tick species in the virus’s circulation. The detection of exposed animals since 2014 suggests that the circulation of the virus beyond the southwestern regions of the Iberian Peninsula could have been more widespread than previously thought.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/8553577","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142860563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guild Dynamics and Pathogen Interactions in Hyalomma Ticks From Algerian Cattle","authors":"Salma Kaoutar Abdelali,&nbsp;Lynda Aissaoui,&nbsp;Apolline Maitre,&nbsp;Elianne Piloto-Sardiñas,&nbsp;Constance Julie,&nbsp;Angélique Foucault-Simonin,&nbsp;Sara Moutailler,&nbsp;Clemence Galon,&nbsp;Lourdes Mateos-Hernández,&nbsp;Dasiel Obregon,&nbsp;Zbigniew Zając,&nbsp;Alejandro Cabezas-Cruz","doi":"10.1155/tbed/5384559","DOIUrl":"https://doi.org/10.1155/tbed/5384559","url":null,"abstract":"<div>\u0000 <p>Ticks are pivotal in transmitting a variety of pathogens that affect both humans and animals. These pathogens often occur in guilds, groups of species that exploit similar resources in similar ways. Although the composition of tick-borne pathogen (TBP) guilds is well-documented, the interactions among pathogens within these guilds remain poorly understood. We hypothesized that abiotic and biotic factors significantly influence the patterns of occurrence and interactions among pathogens within these guilds. To investigate this, we analyzed microfluidic-based high-throughput data on microorganisms from 166 <i>Hyalomma excavatum</i> ticks (94 male and 72 female) collected across different seasons from cattle in the central Algerian steppe using network analysis to uncover complex pathogen–pathogen interaction patterns. We found that female ticks had a higher infection rate (63.9%) with common pathogens such as <i>Rickettsia slovaca</i> (26.4%), unclassified Apicomplexa (22.2%), and <i>Borrelia afzelii</i> (19.4%). Male ticks showed a 56.4% infection rate, with <i>Rickettsia</i> (31.1%) and <i>R. slovaca</i> (16%) being the most prevalent. Notable pathogen–pathogen interactions within guilds were identified, with positive associations such as between <i>R. slovaca</i> and <i>Rickettsia conorii</i> in males, and <i>B. afzelii</i> and <i>Borrelia spielmanii</i> in females, indicating cooperative interactions. Conversely, negative associations, such as between <i>Anaplasma phagocytophilum</i> and <i>Francisella tularensis</i>, suggested competitive exclusion. The observed variation in interaction patterns under different conditions indicates that ecological determinants, both biotic and abiotic, influence pathogen association dynamics within guilds. These findings have significant implications for understanding disease transmission and developing control strategies.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/5384559","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142860302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Prevalence of a Novel Circovirus in the European Hedgehog (Erinaceus europaeus), a Common Species in Decline","authors":"Kevin P. Mulder,&nbsp;Frank Pasmans,&nbsp;Filip van Nieuwerburgh,&nbsp;Naomi Terriere,&nbsp;Moira Kelly,&nbsp;Seline Bregman,&nbsp;Elin Verbrugghe,&nbsp;An Martel","doi":"10.1155/2024/4670252","DOIUrl":"https://doi.org/10.1155/2024/4670252","url":null,"abstract":"<div>\u0000 <p>Hedgehog (<i>Erinaceus europaeus</i>) declines in western Europe have been associated with the emergence of Hedgehog diphtheric disease (HDD), with a probable multifactorial, yet unidentified etiology. We used metagenomic sequencing of cell-free DNA (cfDNA) in hedgehog blood to identify possible causes of HDD. We detected a novel circovirus species in the European hedgehog, providing the first record of a circovirus within the mammalian order Eulipotyphla. The novel circovirus genome exhibits the characteristic circovirus structure, including a functional replicase (REP) and capsid (CAP) gene. Phylogenetic analysis placed all four detected genomes in a monophyletic clade, most closely related to sequences isolated from dogs. Subsequent PCR-based screening of 188 hedgehog liver samples demonstrated a high prevalence (61%) of this circovirus in hedgehogs brought to wildlife rescue centers, however, without any significant association with HDD. Since circoviruses are well known to interfere with host immunity across mammalian and avian taxa, the high level of circovirus detection in hedgehogs warrants further research into the role of this novel virus in hedgehog health.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4670252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Genetic Analysis of Species D Rotaviruses in Pangolin Samples","authors":"Kai Wang,&nbsp;Shasha Liu,&nbsp;Xiaotong Liang,&nbsp;Wanke Hu,&nbsp;Zhenyu Wen,&nbsp;Jiayi Wang,&nbsp;Xianghe Wang,&nbsp;Fuyu An,&nbsp;Ziqiao Chen,&nbsp;Haikuo Yan,&nbsp;Hongmei Yan,&nbsp;Lei Wang,&nbsp;Xiaoai Zhang,&nbsp;Jieshi Yu,&nbsp;Wen-Kang Wei,&nbsp;Yan Hua","doi":"10.1155/tbed/1773821","DOIUrl":"https://doi.org/10.1155/tbed/1773821","url":null,"abstract":"<div>\u0000 <p>Pangolins have been found to carry severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-related coronaviruses. In light of this discovery, interest has been piqued in viromes of these heavily trafficked wild animals. In this study, we performed viral metagenomic sequencing to explore viromes of both confiscated dead pangolins and captive healthy pangolins. Sequence reads of vertebrate-associated viruses in <i>Herpesviridae</i>, <i>Retroviridae</i>, <i>Iridoviridae</i>, <i>Reoviridae</i>, <i>Arenaviridae</i>, and <i>Flaviviridae</i> were detected in confiscated dead pangolins. A novel rotavirus (RV) (<i>Reoviridae</i>), showing a high degree of genetic similarity to the RV species D (RVD) that was previously unreported in mammals, was further confirmed by using reverse transcription-polymerase chain reaction (RT-PCR) and Sanger sequencing. Three out of 18 samples from the confiscated dead pangolins were positive for genomic sequences of the novel RV. Importantly, sequence alignments and phylogenetic analyses demonstrated that these RV strains genetically belonged to the RVD. Nevertheless, these novel RVD strains were divergent from known RVD strains that have been found only in Avian. They formed a separate genetic cluster. Five serial passages were attempted to isolate the RV, but no live virus was obtained. In addition, fecal samples were collected from healthy pangolins (<i>n</i> = 41) in our institution and screened for RVs by viral metagenomic sequencing and RT-PCR. In these fecal samples, neither species D nor previously identified species A RVs were detected. This study reported RVDs in pangolin samples for the first time to our knowledge. Identifiability disagreements between wild and captive pangolins highlight the need for further exploration into pangolin viruses to better understand their emergence and transmission potential.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/1773821","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rotaviruses in Pigeons With Diarrhea: Recovery of Three Complete Pigeon Rotavirus A Genomes and the First Case of Pigeon Rotavirus G in Europe","authors":"Ewa Łukaszuk,&nbsp;Daria Dziewulska,&nbsp;Tomasz Stenzel","doi":"10.1155/tbed/4684235","DOIUrl":"https://doi.org/10.1155/tbed/4684235","url":null,"abstract":"<div>\u0000 <p>Rotaviruses are well-recognized pathogens responsible for diarrhea in humans and various animal species, with <i>Rotavirus A</i> the most often detected and most thoroughly described. Rotaviral disease is an important concern in pathology of pigeons as well, as pigeon rotavirus A was proven to play a major role in young pigeon disease (YPD). However, rotaviruses of other groups have been so far understudied in birds. This paper describes the first finding of Rotavirus G in domestic pigeon in Europe, as well as the recovery of three complete genomes of pigeon rotavirus A with Oxford Nanopore Sequencing. Quantification of pigeon rotavirus A genetic material with droplet digital polymerase chain reaction (PCR) in pigeons suffering from diarrhea and in asymptomatic pigeons was also performed in the frameworks of this study and resulted in determination of statistically highly significant differences between the groups in both detection rate and shedding of the virus. Phylogenetic analysis revealed the close relationship of acquired strains with those originating from pigeons from Europe, North America, Asia, and Australia, indicating a broad geographical spread of pigeon rotaviruses. Results of our research shed more light on occurrence and diversity of <i>Rotavirus</i> species in pigeons.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/4684235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 Seroprevalence in Indoor House Cats From the Lisbon Area During the COVID-19 Pandemic, 2019–2021","authors":"Isa Moutinho,&nbsp;Mafalda Henriques,&nbsp;Sara Cardoso,&nbsp;Teresa da Penha Coutinho,&nbsp;Carlos Penha-Gonçalves,&nbsp;Jocelyne Demengeot,&nbsp;Miguel Castanho,&nbsp;Luís Tavares,&nbsp;Solange Gil,&nbsp;Telmo Nunes,&nbsp;Frederico Aires-da-Silva","doi":"10.1155/tbed/1543922","DOIUrl":"https://doi.org/10.1155/tbed/1543922","url":null,"abstract":"<div>\u0000 <p>The susceptibility of various animal species to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been studied extensively. Cats have garnered significant concern due to their high susceptibility and proximity to humans. This study aimed to evaluate the susceptibility and antibody response in house cats exposed to SARS-CoV-2 when human infection was spreading in the Lisbon area during the 2019–2021 period. A total of 733 serum samples were collected and characterized from cats admitted to the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine at the University of Lisbon (HEV-FMV-ULisboa). All samples were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin M (IgM) and immunoglobulin G (IgG) anti-SARS-CoV-2 whole Spike and receptor-binding domain (RBD) proteins from the Wuhan-Hu-1 isolate and 14.7% (108/733) tested positive, suggesting exposure to the human virus. Surrogate virus neutralization test (sVNT) against the Wuhan-Hu-1 isolate showed that 20.4% of ELISA positive samples (22/108) harbored neutralizing antibodies against the virus. The 22 most promising serum samples were retested using ELISA and sVNT against Alpha, Delta, and Omicron SARS-CoV-2 variants. Notably, these samples exhibited antibodies that were capable of recognizing and neutralizing these variants. Subsequent neutralization assays confirmed that the serum samples effectively inhibited the infection process of Wuhan-Hu-1 D614G, Delta, and Omicron SARS-CoV-2 pseudotyped viruses. Our findings indicate that cats were exposed to SARS-CoV-2 infection during the pandemic period and generated highly effective and broadly neutralizing antibodies against the virus. Although cats have not been demonstrated to significantly contribute to the spread of SARS-CoV-2, their high susceptibility to asymptomatic infection underscores the importance of investment in preventive surveillance measures. In summary, our study reinforces the notion that cats naturally infected with SARS-CoV-2 represent a valuable anthroponotic disease model in house settings and might be a potential source for the development of antibodies against SARS-CoV-2 in tackling future outbreaks with a One Heath perspective.</p>\u0000 </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/1543922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}