Ultrastructural Pathology最新文献_第8页

Investigation of apoptotic and antiproliferative effects of Turkish natural tetraploids Trifolium pratense L. extract on C6 glioblastoma cells via light and electron microscopy. 土耳其天然四倍体三叶草提取物对C6胶质母细胞瘤细胞凋亡和抗增殖作用的光镜和电镜研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2184893

Gamze Tanrıverdi, Aynur Abdulova, Hatice Çölgeçen, Havva Atar, Belisa Kaleci, Tuğba Ekiz-Yılmaz

{"title":"Investigation of apoptotic and antiproliferative effects of Turkish natural tetraploids Trifolium pratense L. extract on C6 glioblastoma cells via light and electron microscopy.","authors":"Gamze Tanrıverdi, Aynur Abdulova, Hatice Çölgeçen, Havva Atar, Belisa Kaleci, Tuğba Ekiz-Yılmaz","doi":"10.1080/01913123.2023.2184893","DOIUrl":"https://doi.org/10.1080/01913123.2023.2184893","url":null,"abstract":"Glioblastoma (GBM) is the most common type of primary brain tumors in adults, characterized by its ability to proliferate rapidly and its tendency to aggressively and strongly invaded the surrounding brain tissue. The standard treatment approach of GBM is surgical resection followed by simultaneous chemotherapy and radiation. However, a significant number of GBM cases develop resistance to currently used chemotherapeutic drugs. Therefore, there is a need for the development of new chemotherapeutic agents. Trifoliumpratense L. is an endemic plant containing various isoflavones such as biochanin A, genistein, daidzein, and formononetin in high concentrations, and it has been shown in various studies that these molecules can function as anticancer agents. The present study was designed to determine the effect of the possible anticarcinogenic effects of the Trifolium pratense L. which grown in our country and to obtain new treatment approaches alternative to the classical treatment protocols applied in the treatment of GBM. C6 glioblastoma cells were cultured with Trifolium pratense L. Cell proliferation, apoptotic cell morphology, and cell structure were evaluated with CCK8, Annexin V, cytochrome c, CD117, and Betatubulin labeling, respectively. And also, investigated effects of this Turkish tetraploid on GBM by TEM. Decreased cell proliferation and increased number of apoptotic cells were observed depending on the increasing doses of Trifolium pratense L. In addition, intense morphological changes were detected depending on increasing doses. In this context, we believe that the plant Trifolium pratense L., may be a new alternative and adjuvant agent for the treatment of GBM.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"160-171"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9396712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The possible effect of lycopene in ameliorating experimentally induced ulcerative colitis in adult male albino rats (A histological, immunohistochemical, and ultrastructural study). 番茄红素对改善成年雄性白化大鼠实验性溃疡性结肠炎的可能影响（组织学、免疫组织化学和超微结构研究）。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 Epub Date: 2023-03-03 DOI: 10.1080/01913123.2023.2185718

Shaimaa Mostafa Kashef, Rania Ibrahim Yassien, Dalia El-Sayed El-Ghazouly

{"title":"The possible effect of lycopene in ameliorating experimentally induced ulcerative colitis in adult male albino rats (A histological, immunohistochemical, and ultrastructural study).","authors":"Shaimaa Mostafa Kashef, Rania Ibrahim Yassien, Dalia El-Sayed El-Ghazouly","doi":"10.1080/01913123.2023.2185718","DOIUrl":"10.1080/01913123.2023.2185718","url":null,"abstract":"Ulcerative colitis (UC) is considered a long-term inflammatory disorder worldwide. Its pathogenesis is associated with reduced antioxidant capacity. Lycopene (LYC) is a powerful antioxidant with strong free radical scavenging property. The present work has done to assess changes of colonic mucosa in induced UC and the possible ameliorative effects of LYC. Forty-five adult male albino rats were randomly divided into four groups: group I (control), group II was given 5 mg/kg/day (LYC) by oral gavage for 3 weeks. Group III (UC) was received single intra-rectal injection of acetic acid. Group IV (LYC+UC) received LYC in same dose and duration as before and acetic acid on 14th day of the experiment. UC group showed loss of surface epithelium with destructed crypts. Congested blood vessels with heavy cellular infiltration were observed. Significant decrease in goblet cell numbers and the mean area percentage of ZO-1 immunoexpression were noticed. Significant increase in the mean area percentage of collagen and the mean area percentage of COX-2 were also noticed. Ultrastructural changes were matched with light microscopic results that showed abnormal destructive columnar and goblet cells. Histological, immunohistochemical, and ultrastructural findings in group IV supported the ameliorative role of LYC against destructive changes induced by UC.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"172-187"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9454225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the effect of the heavy metals cadmium, chromium and lead, alone and in combination on an endothelial cell line. 研究重金属镉、铬和铅单独或联合对内皮细胞系的影响。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2189986

L van Strijp, M Van Rooy, J Serem, C Basson, Hm Oberholzer

{"title":"Investigating the effect of the heavy metals cadmium, chromium and lead, alone and in combination on an endothelial cell line.","authors":"L van Strijp, M Van Rooy, J Serem, C Basson, Hm Oberholzer","doi":"10.1080/01913123.2023.2189986","DOIUrl":"https://doi.org/10.1080/01913123.2023.2189986","url":null,"abstract":"Heavy metals are natural elements characterized by their relatively large atomic mass as well as high density. It can be introduced into the ecosystem by the mining of heavy metals from deep within the earth's crust, thereby exposing the metals into air and water systems. Cigarette smoke is another source of heavy metal exposure and has been shown to have carcinogenic, toxic and genotoxic properties. Cadmium, lead, and chromium are the most abundant metals found in cigarette smoke. In response to tobacco smoke exposure, endothelial cells release inflammatory and pro-atherogenic cytokines that are linked to endothelial dysfunction. Endothelial dysfunction is directly related to the production of reactive oxygen species, leading to endothelial cell loss through necrosis and/or apoptosis. The current study aimed to investigate the effect of cadmium, lead, and chromium, alone and as part of metal mixtures, on endothelial cells. The EA.hy926 endothelial cell line was exposed to different concentrations of each of these metals and their combinations and analyzed using flow cytometric analyses with Annexin V. A clear trend was seen with the Pb + Cr as well as the triple combination group with the significant increase of early apoptotic cells. Scanning electron microscopy was used to study possible ultrastructural effects. Morphological changes observed with scanning electron microscopy included cell membrane damage and membrane blebbing at certain metal concentrations. In conclusion, the exposure of endothelial cells to cadmium, lead, and chromium, caused a disruption in cellular processes and morphology, possibly diminishing the protective ability of endothelial cells.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"205-218"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9766805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An adrenal cortical adenoma with neuroendocrine-type granules mimicking pheochromocytoma. 肾上腺皮质腺瘤伴神经内分泌型颗粒状嗜铬细胞瘤。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2201314

Leonardo Rossi, Carlo Enrico Ambrosini, Liborio Torregrossa, Maria Margherita de Santi, Raffaella Guazzo, Tommaso Simoncini, Alessandra Bacca, Benard Gjeloshi, Francesco Pignatelli, Mattia Iachini, Elisa Loguercio, Chiara Becucci, Gabriele Materazzi

{"title":"An adrenal cortical adenoma with neuroendocrine-type granules mimicking pheochromocytoma.","authors":"Leonardo Rossi, Carlo Enrico Ambrosini, Liborio Torregrossa, Maria Margherita de Santi, Raffaella Guazzo, Tommaso Simoncini, Alessandra Bacca, Benard Gjeloshi, Francesco Pignatelli, Mattia Iachini, Elisa Loguercio, Chiara Becucci, Gabriele Materazzi","doi":"10.1080/01913123.2023.2201314","DOIUrl":"https://doi.org/10.1080/01913123.2023.2201314","url":null,"abstract":"Clinical and biochemical presentation of adrenal tumors may rarely conflict with their histologic features. In the present report, we describe a rare case of adrenal neoplasm clinically and biochemically labeled as pheochromocytoma which at histologic examination resulted adrenal cortical tumor. The neoplasm was examined with the electron microscope which revealed the presence of electron-dense neuroendocrine-type granules next to intracytoplasmic lipid droplets. The patient underwent laparoscopic left adrenalectomy which leads to normalization of 24 h urinary metanephrine and normetanephrine. This exceptional entity should be taken into consideration when the clinical and laboratory features conflict with the histological examination. The pathologist can clarify the mixed nature of the tumor by means of the identification of neuroendocrine granules at the electron microscope examination.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"236-240"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9391414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evening primrose oil attenuates oxidative stress, inflammation, fibrosis, apoptosis, and ultrastructural alterations induced by metanil yellow in the liver of rat: a histological, immunohistochemical, and biochemical study. 月见草油减轻大鼠肝脏氧化应激、炎症、纤维化、细胞凋亡和甲乙二烯黄诱导的超微结构改变:组织学、免疫组织化学和生化研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2189987

Amany Mohamed Shalaby, Rania H Shalaby, Mohamed Ali Alabiad, Doaa I Abdelrahman, Mohammed Alorini, Fatima A Jaber, Shaimaa Mohamed Abdelfattah Hassan

{"title":"Evening primrose oil attenuates oxidative stress, inflammation, fibrosis, apoptosis, and ultrastructural alterations induced by metanil yellow in the liver of rat: a histological, immunohistochemical, and biochemical study.","authors":"Amany Mohamed Shalaby, Rania H Shalaby, Mohamed Ali Alabiad, Doaa I Abdelrahman, Mohammed Alorini, Fatima A Jaber, Shaimaa Mohamed Abdelfattah Hassan","doi":"10.1080/01913123.2023.2189987","DOIUrl":"https://doi.org/10.1080/01913123.2023.2189987","url":null,"abstract":"The food color metanil yellow (Myl) is hazardous to several body systems. Evening primrose oil (EPO) was reported to have anti-inflammatory and anti-oxidant properties. The present work investigated the impact of Myl on the hepatic structure and function of rats and evaluated the protective effect of EPO. Forty adult male rats were divided into four groups: control, EPO (5 g/kg/day), Myl (200 mg/kg/day), and EPO- Myl group. Myl significantly increased liver enzymes, advanced glycation end products (AGE), oxidative stress parameters, pro-inflammatory cytokines, nuclear factor kappa B (NF-κB), and inducible nitric oxide synthase (iNOS). Blood vessels in the liver were dilated and congested, with cellular infiltration around them and associated with fibrosis. The hepatocytes were vacuolated and had dark nuclei. The immunohistochemical expression of iNOS, glial fibrillary acidic protein (GFAP), and Bax was significantly elevated. Ultrastructurally, the hepatocytes showed lipid droplets, irregular condensed nuclei with widened perinuclear space, dilated rER, mitochondria with destructed cristae, and multiple vacuoles. Dilated congested blood sinusoids and collagen fiber bundles were seen between hepatocytes. Interestingly, these alterations were less pronounced in rats co-administrated with EPO and Myl. In conclusion, EPO can protect liver against the toxic effects of Myl due to its anti-inflammatory and anti-oxidant activities.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"188-204"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9403273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteinuria in thrombotic microangiopathy is associated with partial podocytopathy. 血栓性微血管病的蛋白尿与部分足细胞病变有关。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2189341

Megan Moore, Olabisi Afolayan-Oloye, Olaf Kroneman, Wei Li, Hassan D Kanaan, Ping L Zhang

{"title":"Proteinuria in thrombotic microangiopathy is associated with partial podocytopathy.","authors":"Megan Moore, Olabisi Afolayan-Oloye, Olaf Kroneman, Wei Li, Hassan D Kanaan, Ping L Zhang","doi":"10.1080/01913123.2023.2189341","DOIUrl":"https://doi.org/10.1080/01913123.2023.2189341","url":null,"abstract":"Background: Thrombotic microangiopathy (TMA) results in acute kidney injury, but the cause of heavy proteinuria in this disorder is puzzling. The goal of this study was to determine if there were significant effacement of foot processes and CD133-positive hyperplastic podocytes in TMA to explain the proteinuria.Methods: The study included 12 negative controls (renal parenchyma removed from renal cell carcinoma) and 28 thrombotic microangiopathy due to different etiologies. The percent of foot process effacement was estimated, and proteinuria level was obtained for each TMA case. Both groups of cases were stained for CD133 by immunohistochemical method, and the number of positive CD133 in hyperplastic podocytes was counted and analyzed.Results: Nineteen (19) of 28 (68%) TMA cases had nephrotic range proteinuria (urine protein/creatinine >3). Twenty-one (21) of 28 (75%) TMA cases showed positive CD133 staining in scattered hyperplastic podocytes within Bowman's space but was absent in control cases. The percent of foot process effacement (56 ± 4%) correlated with proteinuria (protein/creatinine ratio 4.4 ± 0.6) (r = 0.46, p = .0237) in TMA group.Conclusion: Our data indicate that the proteinuria in TMA can be associated with significant effacement of foot processes. CD133-positive hyperplastic podocytes can be seen in the majority of TMA cases of this cohort, indicating a partial podocytopathy.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"219-226"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9766800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adverse pulmonary effects after oral exposure to copper, manganese and mercury, alone and in mixtures, in a Spraque-Dawley rat model. 在Spraque-Dawley大鼠模型中，单独或混合口服暴露于铜、锰和汞后的肺部不良反应。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2184891

M Draper, Mj Bester, M Van Rooy, Hm Oberholzer

{"title":"Adverse pulmonary effects after oral exposure to copper, manganese and mercury, alone and in mixtures, in a Spraque-Dawley rat model.","authors":"M Draper, Mj Bester, M Van Rooy, Hm Oberholzer","doi":"10.1080/01913123.2023.2184891","DOIUrl":"https://doi.org/10.1080/01913123.2023.2184891","url":null,"abstract":"The rise in respiratory disease has been attributed to an increase in environmental pollution. Heavy metals contribute to environmental contamination via air, water, soil and food. The effects of atmospheric exposure to heavy metals on pulmonary structure and function have been researched, but the effects through drinking water have been neglected. The aim of this study was to investigate the potential in vivo alterations in the pulmonary tissue of male Sprague-Dawley rats after a 28-day oral exposure to copper (Cu), manganese (Mn) and mercury (Hg), alone and in mixtures, at 100 times the World Health Organization's (WHO) safety limit for each heavy metal in drinking water. Forty-eight male Sprague-Dawley rats were randomly divided into eight groups (n = 6): control, Cu, Mn, Hg, Cu + Mn, Cu + Hg, Mn + Hg and Cu, Mn + Hg. The morphology of lung tissue and the bronchioles were evaluated using light- and transmission electron microscopy. For all exposed groups, morphological changes included thickened inter- and intra-alveolar spaces, stratified epithelium, disrupted smooth muscle and early fibrosis and desquamation of the epithelia of the bronchioles to varying degrees. In all exposed groups, ultrastructurally, an increase in disarranged collagen and elastin fibers, nuclear membrane detachment, chromatin condensation, indistinct nucleoli and an increase in collagen fiber disarrangement was observed. This study has identified that oral exposure to Cu, Mn and Hg and as part of mixtures caused pathogenesis due to inflammation, cellular damage and fibrosis with Mn + Hg being the most potent heavy metal group.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"146-159"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9396709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome: a case report. 线粒体脑肌病合并乳酸酸中毒和卒中样发作综合征1例报告。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2184892

Yong-Xin Ru, Li Ying, Shu-Xu Dong, Hui-Ming Yi, Liu Jing, Zhang Yongqiang

{"title":"Mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome: a case report.","authors":"Yong-Xin Ru, Li Ying, Shu-Xu Dong, Hui-Ming Yi, Liu Jing, Zhang Yongqiang","doi":"10.1080/01913123.2023.2184892","DOIUrl":"https://doi.org/10.1080/01913123.2023.2184892","url":null,"abstract":"A biopsy of gastrocnemius muscle from a patient with mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS) syndrome was studied histologically in semithin sections stained by hematoxylin-and-eosin (H&E) and toluidine blue, and ultrathin sections by transmission electron microscopy (TEM). H&E stain demonstrated typical ragged-red fibers (RRFs) and affected fibers in fascicles. Toluidine-blue stain showed an irregular meshwork in the center of RRFs. TEM demonstrated damaged myofibrils and variations in mitochondrial structure in RRFs and affected fibers. Dense mitochondria were compacted with cristae and pleomorphic electron-dense inclusions. Lucent mitochondria included paracrystalline inclusions with a parking lot appearance. At high magnification, the paracrystalline inclusions were composed of plates that paralleled and connected with mitochondrial cristae. These observations indicated that electron-dense granular and paracrystalline inclusions resulted from cristal degeneration and overlapping in mitochondria in MELAS syndrome.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"227-235"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9398134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study between the possible protective role of melatonin versus its combination with adipose derived-mesenchymal stem cells on experimentally induced diabetic retinopathy in adult male albino rats (Histological and immunohistochemical study). 褪黑素与其联合脂肪源间充质干细胞对实验性诱导的成年雄性白化大鼠糖尿病视网膜病变可能保护作用的比较研究(组织学和免疫组织化学研究)。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-05-04 DOI: 10.1080/01913123.2023.2184890

Samar Reda, Ghada A Elsammak, Tamer G Elsayed, Samar Abdelaziz Mostafa

{"title":"A comparative study between the possible protective role of melatonin versus its combination with adipose derived-mesenchymal stem cells on experimentally induced diabetic retinopathy in adult male albino rats (Histological and immunohistochemical study).","authors":"Samar Reda, Ghada A Elsammak, Tamer G Elsayed, Samar Abdelaziz Mostafa","doi":"10.1080/01913123.2023.2184890","DOIUrl":"https://doi.org/10.1080/01913123.2023.2184890","url":null,"abstract":"Diabetes mellitus is a metabolic disorder that can cause numerous ocular issues as well as long-term effects. In our study, we evaluate the effect of melatonin on the diabetic retinal alterations in male albino rats to the effect of melatonin combined with stem cells. 50 adult male rats were equally divided into four groups control, diabetic, melatonin, and melatonin plus stem cells. STZ, 65 mg/kg in phosphate buffered was administered intraperitoneally as a bolus to diabetic group of rats. After inducing diabetes, melatonin (10 mg/kg b.wt./day) was administered orally to the melatonin group for 8 weeks. The stem cell and melatonin group got the same dosage of melatonin as the prior group. They received an intravenous injection of (3?×?106 cell) adipose-derived MSC suspended in phosphate-buffered saline at same time of melatonin ingestion. Animals from all groups had their fundics examined. Following the injection of stem cells, samples of rat retina were collected for light and electron microscopy analyses. H&E and immunohistochemically stained sections revealed a slight improvement in group (III). At the same time, group (IV) results were comparable to those of the control group, which was supported by the findings of an electron microscope. Neovascularization was visible on fundus examination in group (II), whereas it was less noticeable in group (III) and group IV. Melatonin mildly improved the histological structure of the retina in diabetic rats, and when it was combined with adipose-derived MSC, it considerably improved the diabetic alterations.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"131-145"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9454227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of hazards of electronic -cigarette's liquid refill on testes of mice, complemented by histopathological and chromatographic analysis. 通过组织病理学和色谱分析评估电子烟液体笔芯对小鼠睾丸的危害。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-02-25 DOI: 10.1080/01913123.2023.2178569

Raghda Elsherif, Nora Z Abdellah, Ola A Hussein, Eman S Shaltout

{"title":"Evaluation of hazards of electronic -cigarette's liquid refill on testes of mice, complemented by histopathological and chromatographic analysis.","authors":"Raghda Elsherif, Nora Z Abdellah, Ola A Hussein, Eman S Shaltout","doi":"10.1080/01913123.2023.2178569","DOIUrl":"10.1080/01913123.2023.2178569","url":null,"abstract":"Electronic-cigarettes (e-cigarettes) are devices designed to become an alternative to classic cigarettes. Vaping of e-cigarettes and their recharge liquid have become extremely popular among the adolescents; however, its safety is not well established. Evaluation of the components of e-cigarette liquid and their potential effects on testis of adult male mice. This aim will be fulfilled by histological, ultrastructural, and immunohistochemical analysis of mice testis biopsies. Twenty mice were allocated into two groups of equal size. The control group was given regular saline, whereas the treated group was given e-liquid (contains 3 mg of nicotine/kg of body weight) both groups daily intraperitoneally injected for 3 weeks. Analysis of e-liquid by Gas Chromatography-Mass Spectrometric GC/MS demonstrated nicotine, phenol, vanillin, aldehydes, and pyrethroid insecticide. Evaluation of oxidative stress parameters revealed significant reduction of SOD and GPx. Histological results revealed a significant reduction in the height of seminiferous tubules, sloughing of spermatogenic cells, most cells being dark and pyknotic, and thickening of the interstitium with accumulation of PAS positive exudate. Most spermatogenic cells showed degenerative changes as rarefied cytoplasm, ill-defined electron-dense nuclei, and elongated spermatid showed deformity of ectoplasmic specialization. Immunohistochemical studies revealed a significant increase in caspase-3 positive cells and a significant reduction of area % of E-cadherin. The analysis of an available E-liquid demonstrated potentially harmful chemicals that are not shown in the labeling of the product. E-liquid appears to impair anti-oxidant defense and cause degenerative changes in the body and disruption of blood testes barrier BTB. So, e-cigarettes cannot be regarded as a non-harmful smoking replacement.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-14"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9336789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0