链脲佐菌素诱导糖尿病大鼠睾丸结构变化及蜂王浆可能的保护作用:光镜和电镜研究。

IF 1.1 4区 医学 Q4 MICROSCOPY
Ultrastructural Pathology Pub Date : 2024-01-02 Epub Date: 2024-01-09 DOI:10.1080/01913123.2023.2277170
Eman A El Helew, Wafaa S Hamed, Amal M Moustafa, Zeinab A Sakkara
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引用次数: 0

摘要

糖尿病(DM)是最常见的代谢性疾病之一,会对包括睾丸在内的身体许多器官造成损害。蜂王浆(RJ)是一种具有抗氧化、抗炎和抗糖尿病特性的蜜蜂产品。本研究旨在评估链脲佐菌素(STZ)诱导的糖尿病大鼠睾丸微观结构的变化,以及RJ可能的保护作用。将60只成年雄性白化大鼠分为三组。第一组为对照组,第二组为STZ组,第三组为STZ+RJ组。第二组接受单剂量STZ(50 mg/kg)。第三组与第二组一样接受单剂量STZ,然后通过胃内管口服RJ,剂量为(100 mg/kg/天) 糖尿病确诊数周后。进行了光镜和电子显微镜研究。第二组显示了影响曲精小管的显著结构变化,生发上皮严重减少,管腔中成熟精子丢失。间质组织显示间质细胞退化,血管充血。第二组的Mallory三色染色切片显示胶原纤维的量显著增加。第三组显示高度保存的睾丸结构几乎与对照组相似,只有少数小管受损。总之,RJ通过其抗氧化作用保护睾丸结构免受糖尿病氧化应激的破坏,从而保持男性生育能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural changes in testes of Streptozotocin induced diabetic rats and possible protective effect of royal jelly: light and electron microscopic study.

Diabetes mellitus (DM) is one of the most common metabolic diseases causing damage in many organs in the body including the testes. Royal Jelly (RJ) is one of the honey bee products that has antioxidant, anti-inflammatory and antidiabetic properties. This study was performed to evaluate the changes in the microscopic structure of the testes in Streptozotocin (STZ)-induced diabetic rats, and the possible protective role of RJ. 60 adult male albino rats were divided into three groups. Group I Control group, Group II STZ group, and Group III STZ+RJ group. Group II received a single dose of STZ (50 mg/kg) by intraperitoneal injection. Group III received a single dose of STZ as in the second group then received RJ orally by intragastric tube in dose of (100 mg/kg/day) for 4 weeks after confirmation of diabetes. Light and electron microscopic studies were performed. Group II revealed marked structural changes affecting seminiferous tubules with sever reduction in germinal epithelium and loss of mature spermatozoa in their lumina. The interstitial tissue revealed degenerated Leydig cells and congested blood vessels. Mallory trichrome stained section of group II revealed marked increase in the amount of collagen fibers. Group III revealed highly preserved testicular architecture almost near to that appeared in the control group except few tubules that were damaged. In conclusion, RJ protected the testicular structure from the damaging effect of diabetic oxidative stress through its antioxidant effect thus preserving male fertility.

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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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