Ultrastructural Pathology最新文献

The impact of chronic high-dose DHEA administration on liver, kidney, heart, and skeletal muscle tissues in young male rats with protective effects of vitamin E. 慢性大剂量脱氢表雄酮给药对具有维生素E保护作用的年轻雄性大鼠肝、肾、心脏和骨骼肌组织的影响。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-10-12 DOI: 10.1080/01913123.2026.2742301

Gozde Erkanli Senturk, Hakan Sahin, Fatma Beyza Sağ, Zehra Sezer, Onder Huseyinbas

{"title":"The impact of chronic high-dose DHEA administration on liver, kidney, heart, and skeletal muscle tissues in young male rats with protective effects of vitamin E.","authors":"Gozde Erkanli Senturk, Hakan Sahin, Fatma Beyza Sağ, Zehra Sezer, Onder Huseyinbas","doi":"10.1080/01913123.2026.2742301","DOIUrl":"https://doi.org/10.1080/01913123.2026.2742301","url":null,"abstract":"Dehydroepiandrosterone (DHEA) is a steroidal hormone that is increasingly used as a dietary supplement, particularly among young men. Despite its widespread use, information regarding its potential adverse effects on vital organs remains limited. Therefore, the present study aimed to investigate the chronic effects of high‑dose DHEA supplementation on the liver, kidneys, heart, and muscle tissues in young male rats and to evaluate the potential protective role of vitamin E (Vit E) against DHEA‑induced tissue damage. Male Sprague Dawley rats were divided into three groups: Control, DHEA‑treated, and DHEA + Vit E‑treated groups. Following chronic administration of DHEA, tissue samples from the liver, kidneys, heart, and skeletal muscle were collected. Histological, ultrastructural, and biochemical analyses were performed to evaluate possible tissue injury and to assess the potential protective effects of Vit E supplementation. DHEA administration resulted in significant histopathological, ultrastructural, and biochemical alterations, indicating tissue injury. In contrast, rats receiving both DHEA and Vit E demonstrated significantly reduced levels of damage compared with the DHEA only group. The findings revealed that prolonged high‑dose DHEA supplementation caused notable injury in the liver and kidneys, whereas no significant damage was observed in muscle tissues. Chronic high‑dose DHEA supplementation may induce detrimental effects, particularly in the liver and kidneys, in young male rats. However, Vit E supplementation may partially mitigate these adverse effects, suggesting a potential protective role against DHEA‑induced organ toxicity.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-17"},"PeriodicalIF":1.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perfusion quality does not necessarily predict ultrastructural preservation after hyperosmotic brain perfusion. 灌注质量不一定能预测高渗脑灌注后超微结构的保存。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-10-11 DOI: 10.1080/01913123.2026.2745999

Andria Slaughter, Macy Garrood, Katelyn Hedden, Allison Sowa, William Janssen, Emma L Thorn, Claudia De Sanctis, Kurt Farrell, John F Crary, Andrew T McKenzie

{"title":"Perfusion quality does not necessarily predict ultrastructural preservation after hyperosmotic brain perfusion.","authors":"Andria Slaughter, Macy Garrood, Katelyn Hedden, Allison Sowa, William Janssen, Emma L Thorn, Claudia De Sanctis, Kurt Farrell, John F Crary, Andrew T McKenzie","doi":"10.1080/01913123.2026.2745999","DOIUrl":"https://doi.org/10.1080/01913123.2026.2745999","url":null,"abstract":"Perfusion fixation is widely used in neuroscience to prepare mammalian brain tissue for histological and ultrastructural analysis. Perfusion protocols are commonly assessed using macroscopic indicators such as gross appearance and neuroimaging, which assess the extent to which perfusate has been distributed throughout the brain. There is a critical need to determine to what extent these metrics can accurately predict high-quality ultrastructural preservation, particularly as new perfusion protocols are developed for connectomics. In this technical report, we describe evidence that these two measures can be decoupled by the addition of dehydrating agents to the perfusate solution. In three human brain donors and one canine brain donor perfused with a fixative solution containing 10% mannitol and 10% polyethylene glycol 35 kDa, macroscopic and radiological indicators of perfusion quality appeared adequate or favorable. However, electron microscopy revealed expanded extracellular space, shrunken cellular processes, and distorted cell membranes, consistent with an osmotic shock artifact resulting from severe hyperosmotic dehydration. Similar ultrastructural artifacts were observed in a canine brain donor perfused with 20% mannitol in 20% neutral buffered formalin without PEG. We compare these ultrastructural findings with findings from previously reported cases perfused with standard neutral buffered formalin without osmotic additives. These findings illustrate a risk of optimizing brain perfusion protocols designed to preserve neural circuitry based on macroscopic or radiological perfusion quality metrics alone, since these metrics can be satisfied while the ultrastructure is severely compromised.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Langerhans cell sarcoma in the central nervous system mimicking a meningioma: case report and literature review. 模仿脑膜瘤的中枢神经系统朗格汉斯细胞肉瘤：1例报告及文献复习。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-10-05 DOI: 10.1080/01913123.2026.2739245

Jorge Alberto Vargas-Meza, Mariana Danery Pacheco-Pérez, Guillermo Ernesto Corredor Alonso, Hugo Ricardo Dominguez-Malagon

{"title":"Langerhans cell sarcoma in the central nervous system mimicking a meningioma: case report and literature review.","authors":"Jorge Alberto Vargas-Meza, Mariana Danery Pacheco-Pérez, Guillermo Ernesto Corredor Alonso, Hugo Ricardo Dominguez-Malagon","doi":"10.1080/01913123.2026.2739245","DOIUrl":"https://doi.org/10.1080/01913123.2026.2739245","url":null,"abstract":"Langerhans cell sarcoma (LCS) and Langerhans cell histiocytosis (LCH) are proliferative conditions involving cells that exhibit Langerhans cell features. While LCH can have a variable behavior ranging from benign hyperplasia to a fatal multisystemic disease, LCS represents a rare, aggressive, high-grade malignancy. Central Nervous System (CNS) involvement is rare, and extra-axial lesions can frequently mimic meningiomas or carcinomatous infiltrates, posing a significant diagnostic challenge. We report the case of a 31-year-old male who presented with severe holocranial headache, projectile vomiting, and focal neurological deficits. Brain magnetic resonance imaging revealed a right supratentorial extra-axial mass, initially misdiagnosed as a meningioma. Histopathological examination demonstrated a proliferation of cells with high mitotic activity and conspicuous atypia. Immunohistochemistry showed positivity for S100, CD68, CD1a, and Langerin. Crucially, ultrastructural analysis by electron microscopy revealed the pathognomonic presence of Birbeck granules, confirming the diagnosis of LCS. This case highlights an exceptional presentation of CNS LCS mimicking a meningioma radiologically. It underscores the absolute necessity of integrating clinical, immunohistochemical, and ultrastructural findings to accurately differentiate high-grade histiocytic proliferations from other dural-based non-meningothelial lesions.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-6"},"PeriodicalIF":1.2,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous ultrastructure reveals potential biomarkers of ventricular arrhythmias in inflammatory cardiomyopathy. 皮肤超微结构揭示炎症性心肌病室性心律失常的潜在生物标志物。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-10-02 DOI: 10.1080/01913123.2026.2733314

Andrea Villatore, Giovanni Paolino, Emanuela Sergianni, Mariachiara Canadeo, Maria Carla Panzeri, Paolo Della Bella, Stefano Calvieri, Santo Raffaele Mercuri, Giovanni Peretto

{"title":"Cutaneous ultrastructure reveals potential biomarkers of ventricular arrhythmias in inflammatory cardiomyopathy.","authors":"Andrea Villatore, Giovanni Paolino, Emanuela Sergianni, Mariachiara Canadeo, Maria Carla Panzeri, Paolo Della Bella, Stefano Calvieri, Santo Raffaele Mercuri, Giovanni Peretto","doi":"10.1080/01913123.2026.2733314","DOIUrl":"https://doi.org/10.1080/01913123.2026.2733314","url":null,"abstract":"Inflammatory cardiomyopathy (ICM), resulting from persistent myocardial inflammation, is associated with life-threatening ventricular arrhythmias (VA). Recent studies suggested overlap between ICM-associated VA and desmosomal diseases affecting the intercalated discs (ID), which are critical for electromechanical coupling. Genetic variants in desmosomal genes cause arrhythmogenic cardiomyopathies and genodermatoses, supporting a potential link between desmosomal dysfunction and arrhythmic risk. This single-center pilot study explored cutaneous abnormalities in patients with ICM complicated by VA, focusing on desmosomal alterations in skin tissue. Four patients (mean age 56 ± 6 years, 50% male) with cardiac magnetic resonance- and endomyocardial biopsy-proven ICM, documented VA, and available myocardial and cutaneous tissue were retrospectively evaluated. Two patients carried genetic variants (DSP and FLNC), while two had systemic autoimmune diseases. Transmission electron microscopy revealed consistent ultrastructural abnormalities in cardiac biopsies, including widened intercellular cleft width (ICW), a marker associated with arrhythmic risk. Similar abnormalities were observed in cutaneous biopsies, including small, narrow, or clustered desmosomes and dermal-epidermal junction detachments. Patients with greater cutaneous desmosomal coupling variability exhibited more complex VA, lower left ventricular ejection fraction, and a genetic etiology. These findings suggest that cutaneous desmosomal abnormalities may mirror cardiac changes and represent a potential biomarker for arrhythmic risk stratification.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-5"},"PeriodicalIF":1.2,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin and bradykinin potentiating peptide protect against Busulfan induced liver and kidney damage in Sprague Dawley rats: an ultrastructural study. 槲皮素和缓激肽增强肽对布苏凡诱导大鼠肝肾损伤的超微结构研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-09-29 DOI: 10.1080/01913123.2026.2739246

Khaled Radad, Maysson Mokhtar, Refaat A Eid, Mubarak Al-Shraim, Hossam Omar, Sary Khalil Abdel-Ghafar

{"title":"Quercetin and bradykinin potentiating peptide protect against Busulfan induced liver and kidney damage in Sprague Dawley rats: an ultrastructural study.","authors":"Khaled Radad, Maysson Mokhtar, Refaat A Eid, Mubarak Al-Shraim, Hossam Omar, Sary Khalil Abdel-Ghafar","doi":"10.1080/01913123.2026.2739246","DOIUrl":"https://doi.org/10.1080/01913123.2026.2739246","url":null,"abstract":"Busulfan (BUS), a chemotherapeutic agent used to treat chronic myeloid leukemia, is associated with hepatotoxicity and nephrotoxicity. This study used transmission electron microscope (TEM) to characterize BUS-induced ultrastructural alterations in the liver and kidney of rats, and to evaluate the protective effects of quercetin (QCT), bradykinin potentiating peptide (BPP) and their combination. To our knowledge, these ultrastructural changes have not been previously reported. Rats received two BUS doses (10 mg/kg b.w.) at a 21-day interval. BUS caused marked hepatic alterations, including mitochondrial swelling with loss of cristae, rough endoplasmic reticulum (RER) dilatation, sinusoidal endothelial cell damage and increased collagen synthesis. Renal changes included thrombotic microangiopathy affecting the glomerular capillary, thickened glomerular basement membranes, reactive changes of podocyte and parietal epithelial cells, mesangial cell proliferation, Bowman's space dilatation, and increased periglomerular collagen deposition. Concomitant treatment with QCT markedly reduced most hepatic alterations, whereas the BUS+BPP group exhibited comparatively limited protection. The combined QCT+BPP treatment produced the greatest hepatic protection. In the kidney, co-treatment with QCT partially ameliorated BUS-induced damage, while BUS+BPP and BUS+QCT+BPP restored ultrastructural features to near normal. In conclusions, these findings demonstrate that BUS induces marked ultrastructural injury in the liver and kidney, QCT, BPP, particularly in combination, provide substantial protection against these alterations.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Sodium Nitrite on the cerebellar cortex and the possible role of Quercetin in adult male albino rats. 亚硝酸钠对成年雄性白化大鼠小脑皮质的影响及槲皮素的可能作用。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-09-25 DOI: 10.1080/01913123.2025.2541622

Maha Amin Abdallah Khattab, Samia Adel Abd El-Baset, Aya Ebrahim Abdelfattah Mohammed, Ghada A Elsammak

{"title":"Effect of Sodium Nitrite on the cerebellar cortex and the possible role of Quercetin in adult male albino rats.","authors":"Maha Amin Abdallah Khattab, Samia Adel Abd El-Baset, Aya Ebrahim Abdelfattah Mohammed, Ghada A Elsammak","doi":"10.1080/01913123.2025.2541622","DOIUrl":"https://doi.org/10.1080/01913123.2025.2541622","url":null,"abstract":"The cerebellum is particularly vulnerable to intoxication and poisoning due to its complex structure. Sodium Nitrite is commonly used as a food additive, as well as in fertilizers and rodenticides. Quercetin, a widely distributed flavonoid in nature, has been investigated for its potential protective effects. This research aimed to evaluate the histological and biochemical changes in the cerebellar cortex of adult male albino rats resulting from Sodium Nitrite exposure, and to assess the protective role of Quercetin and the reversibility of these changes after stopping Sodium Nitrite. Fifty-four healthy adult male albino rats were divided into four groups: I) Control group, II) Sodium Nitrite treated group, III) Sodium Nitrite and Quercetin treated group, and IV) Withdrawal group. Tissue sections from the cerebellar cortex were stained with H&E and examined microscopically and ultrastructurally. Expression levels of GFAP and Caspase 3 in the cerebellar tissue were assessed immunohistochemically, while MDA and SOD levels were measured. The results showed that Sodium Nitrite significantly disrupted cerebellar tissue structure, leading to increased expression of GFAP and Caspase 3. Quercetin supplementation alleviated apoptosis in the cerebellar tissue and reduced the expression of both GFAP and Caspase 3. The withdrawal group exhibited only slight improvement. In conclusion, Quercetin supplementation effectively alleviated cerebellar neurotoxicity induced by Sodium Nitrite while recovery after discontinuation of Sodium Nitrite was limited.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-18"},"PeriodicalIF":1.2,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149040738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mineralogical analysis in the differential diagnosis of silicosis and sarcoidosis : diagnostic applications in pulmonary pathology. 矿物学分析在矽肺和结节病鉴别诊断中的应用。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-09-23 DOI: 10.1080/01913123.2026.2733309

Matthieu Barthas, Vincent Bonneterre, Mickael Catinon, Dominique Tripodi

{"title":"Mineralogical analysis in the differential diagnosis of silicosis and sarcoidosis : diagnostic applications in pulmonary pathology.","authors":"Matthieu Barthas, Vincent Bonneterre, Mickael Catinon, Dominique Tripodi","doi":"10.1080/01913123.2026.2733309","DOIUrl":"https://doi.org/10.1080/01913123.2026.2733309","url":null,"abstract":"Silicosis and thoracic sarcoidosis are both diffuse interstitial lung diseases, one occupational and the other idiopathic. The clinical and pathological findings of these two conditions show strong similarities, which can make diagnosis difficult. The main objective of this study is to present the mineralogical analysis techniques that can be performed on lung samples to provide new insights for distinguishing silicosis from thoracic sarcoidosis. A systematic review of the literature was conducted using the PubMed and Web of Science databases. A systematic review of the literature was conducted using the PubMed and Web of Science databases. Twenty-six articles published between 1975 and 2024 were included in the final analysis. The review identified seven mineralogical analysis techniques. Scanning and transmission electron microscopy are the oldest techniques, applicable to lung samples following digestion or in situ preparation. Methods for automating these techniques are emerging. X-ray diffraction and ICP-AES are techniques usable only on samples following digestion preparation and provide quantitative information only in terms of atomic compounds. Micro-PIXE and LIBS are more sophisticated techniques enabling the visualization of the distribution of atoms (silicon, aluminum, etc.) within tissues. Mineralogical analysis techniques, particularly electron microscopy coupled with elemental analysis and LIBS, represent valuable adjuncts in pulmonary pathology for identifying inorganic particles and supporting the differential diagnosis between pneumoconiosis and sarcoidosis in diagnostically challenging cases.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-16"},"PeriodicalIF":1.2,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149031383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bruce Mackay, MD, PhD, founding member of Society for Ultrastructural pathology: in memoriam - his legacy lives on. 布鲁斯·麦凯，医学博士，超微结构病理学学会创始成员：纪念-他的遗产将永存。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-06 DOI: 10.1080/01913123.2025.2610867

John Hicks, Josep Lloreta-Trull

引用次数: 0

Gold nanoparticles induced histological, ultrastructural, and functional changes of the adult rat testis. Is it nano-size dependent? 金纳米颗粒诱导成年大鼠睾丸组织、超微结构和功能的改变。是否依赖于纳米尺寸？

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1080/01913123.2025.2580526

Sahar Galal Gouda, Dalia Muhammad Abd-Elhalim, Nema Soliman

{"title":"Gold nanoparticles induced histological, ultrastructural, and functional changes of the adult rat testis. Is it nano-size dependent?","authors":"Sahar Galal Gouda, Dalia Muhammad Abd-Elhalim, Nema Soliman","doi":"10.1080/01913123.2025.2580526","DOIUrl":"10.1080/01913123.2025.2580526","url":null,"abstract":"Gold nanoparticles (AuNPs) have diverse medical applications, but concerns rose about toxicity. The goal of this work was to evaluate the harmful effects of AuNPs (15-nm and 40-nm) on adult rat testes. Twenty-one male rats were assigned into three groups and received the following treatments via gavage: distilled water as a control, 15-nm AuNPs group, and 40-nm AuNPs group. After 28 days, animals were sacrificed. Testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels were measured. Testicular tissue's levels of malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) were measured. Sperm morphology and count were assessed. Testicular histology was assessed using toluidine blue stain, periodic acid-Schiff (PAS), and hematoxylin and eosin (H&E). Transmission electron microscopy was used to evaluate the testicular ultrastructure. The findings showed that both AuNPs groups saw a substantial (p ≤ .05) rise in FSH, a drop in testosterone, an increase in MDA, and a decrease in GSH. The 15-nm AuNPs group saw a substantial decrease in sperm count. Histological findings showed that the 15-nm AuNPs group had more atrophic seminiferous tubules and a substantial (p ≤ .05) reduction in seminiferous epithelial height. Electron microscopy showed germ cells with degraded mitochondria and cytoplasmic vacuoles. Testicular structure and function were both negatively impacted by AuNPs, with the effects being more pronounced for 15 nm AuNPs.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"37-55"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145402085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glomerular epithelial cell differentiation and aberration evaluated using PAX8 and WT1 immunostains. PAX8和WT1免疫染色评价肾小球上皮细胞分化和畸变。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2025-11-28 DOI: 10.1080/01913123.2025.2594992

Ping L Zhang, Alexander Zarouk, Brandon D MeCalf, Olaf Kroneman, Dilip Samarapungavan, Jamal Abukhaled, Garmpala H Reddy, Sami Zarouk, Wei Li, Hassan D Kanaan

{"title":"Glomerular epithelial cell differentiation and aberration evaluated using PAX8 and WT1 immunostains.","authors":"Ping L Zhang, Alexander Zarouk, Brandon D MeCalf, Olaf Kroneman, Dilip Samarapungavan, Jamal Abukhaled, Garmpala H Reddy, Sami Zarouk, Wei Li, Hassan D Kanaan","doi":"10.1080/01913123.2025.2594992","DOIUrl":"10.1080/01913123.2025.2594992","url":null,"abstract":"Paired box 8 (PAX8) and Wilms' tumor 1 (WT1) of cap mesenchyme play important roles in tubular epithelial transition and podocyte maturation of the kidney. Parietal epithelial cells (PEC) progenitors demonstrate activity of these two factors in mature glomeruli. PECs can be activated into spindle cell crescents in crescentic glomerulonephritis (CreGN) or into podocyte hyperplasia in collapsing glomerulopathy (ColGN) based on histologic evidence. The study aimed to use PAX8 and WT1 stains to demonstrate the two transitional processes of mesonephros/metanephros and aberrant in CreGN, and ColGN. Immunohistochemical (IHC) stains for both PAX8 and WT1 were performed in fetal mesonephros/metanephros, adult negative controls, CreGN, and ColGN. Expressive patterns of PAX8 and WT1 in mesonephros/early fetal kidneys, adult kidneys, and aberrant glomerular changes were evaluated. PAX8 showed continuous nuclear expression in the parietal epithelium of mesonephros/metanephros, adult kidneys, & primordial podocytes, and consistent positivity in all renal tubules. WT1 showed strong nuclear and cytoplasmic expression within mature podocytes at all stages, despite some staining of WT1 in parietal epithelium. Cellular crescents and hyperplastic podocytes were strongly positive for PAX8, and only weakly positive in the nuclei of WT1. Our data support that PAX8+ PEC involve in mesenchymal-epithelial transition (MET) in renal tubules, a possible induction of early podocyte development, and aberrant epithelial proliferation of CreGN and ColGN. In addition, WT1 is fully devoted to podocyte maturation and is also partially involved in the aberrant epithelial proliferation of the two glomerular diseases.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"82-91"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145639893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0