Ultrastructural Pathology最新文献

The possible effects of chronic administration of amiodarone hydrochloride on the seminiferous tubules of adult male albino rats: histological and biochemical study. 长期服用盐酸胺碘酮对成年雄性白化大鼠曲细精管的可能影响：组织学和生化研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-26 DOI: 10.1080/01913123.2024.2407330

Amany M Abo-Ouf, Amany F Mohamed, Heba Abdelnaser Aboelsoud, Ayman Geddawy, Heba F Ibrahim

{"title":"The possible effects of chronic administration of amiodarone hydrochloride on the seminiferous tubules of adult male albino rats: histological and biochemical study.","authors":"Amany M Abo-Ouf, Amany F Mohamed, Heba Abdelnaser Aboelsoud, Ayman Geddawy, Heba F Ibrahim","doi":"10.1080/01913123.2024.2407330","DOIUrl":"https://doi.org/10.1080/01913123.2024.2407330","url":null,"abstract":"Amiodarone hydrochloride is an antiarrhythmic agent that is widely prescribed. However, it has serious side effects that approximately affect the whole body organs. In our study, we aimed to assess the possible effects of chronic administration of two different doses of amiodarone hydrochloride on the oxidative and inflammatory parameters as well as the histological morphology and ultrastructure of the seminiferous tubules of adult male albino rats. Forty rats were divided into four groups; Control group 1: each rat did not receive any drugs at all. Control group 2: each rat received 3 ml of 0.16% methylcellulose, orally and daily for 4 weeks. Low dose amiodarone group: each rat received 3 ml of 0.16% methylcellulose contained 3.6 mg amiodarone, orally and daily for 4 weeks. High dose amiodarone group: each rat received 3 ml of 0.16% methylcellulose contained 7.2 mg amiodarone, orally and daily for 4 weeks. Blood samples were collected for measuring serum levels of malondialdehyde, superoxide dismutase, interleukin-6 and tumor necrosis factor-alpha. Testes specimens were examined to assess the morphological changes and the level of expression of caspase-3 apoptotic marker. The results indicated that; amiodarone hydrochloride could induce a dose-dependent toxicity, causing oxidative stress, inflammation, cellular degeneration, deposition of collagen and enhanced apoptosis in the seminiferous tubules.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivation of nucleases with peroxidation damages induced by a menadione: ascorbate combination devastates human prostate carcinomas: ultrastructural aspects. 由甲萘醌：抗坏血酸盐组合诱导的核酸酶再激活与过氧化损伤对人类前列腺癌的破坏：超微结构方面。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI: 10.1080/01913123.2024.2379300

Jacques Gilloteaux, James M Jamison, Jack L Summers, Henryk S Taper

{"title":"Reactivation of nucleases with peroxidation damages induced by a menadione: ascorbate combination devastates human prostate carcinomas: ultrastructural aspects.","authors":"Jacques Gilloteaux, James M Jamison, Jack L Summers, Henryk S Taper","doi":"10.1080/01913123.2024.2379300","DOIUrl":"10.1080/01913123.2024.2379300","url":null,"abstract":"Introduction: Xenografts of androgen-independent human DU145 prostate metastatic carcinomas implanted in nu/nu male mice have revealed a significant survival after a prooxidant anticancer treatment consisting of a combination of menadione bisulfite and sodium ascorbate (VK3:VC).Methods: Implanted samples of diaphragm carcinomas from longest survived mice from either oral, intraperitoneal (IP), or both oral and IP treatment groups were assessed with light, scanning, and transmission electron microscopy to analyze morphologic damages.Results: Compared with previous fine structure data of in vitro untreated carcinomas, the changes induced by oral, IP, and oral with IP VK3:VC treatment dismantled those xenografts with autoschizis, and necrotic atrophy was accomplished by cell's oxidative stress whose injuries were consequent to reactivated deoxyribonucleases and ribonucleases. Tumor destructions resulted from irreversible damages of nucleus components, endoplasmic reticulum, and mitochondria there. Other alterations included those of the cytoskeleton that resulted in characteristic self-excisions named \" autoschizis.\" All these injuries lead resilient cancer cells to necrotic cell death.Conclusion: The fine structure damages caused by VK3:VC prooxidant combination in the human DU145 prostate xenografts confirmed those shown in vitro and of other cell lines with histochemistry and biomolecular investigations. These devastations incurred without damage to normal tissues; thus, our data brought support for the above combination to assist in the treatment of prostate cancers and other cancers.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential protective effect of propolis on diabetic nephropathy induced by streptozotocin in adult albino rats. 蜂胶对链脲佐菌素诱导的成年白化大鼠糖尿病肾病的潜在保护作用。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-01 DOI: 10.1080/01913123.2024.2386009

Shaimaa M Hafez, Heba F Ibrahim, Shaimaa R Abdelmohsen, Noha A E Yasin, Yara S Abouelela, Heba Abdelnaser Aboelsoud

{"title":"The potential protective effect of propolis on diabetic nephropathy induced by streptozotocin in adult albino rats.","authors":"Shaimaa M Hafez, Heba F Ibrahim, Shaimaa R Abdelmohsen, Noha A E Yasin, Yara S Abouelela, Heba Abdelnaser Aboelsoud","doi":"10.1080/01913123.2024.2386009","DOIUrl":"10.1080/01913123.2024.2386009","url":null,"abstract":"Diabetes mellitus is a common metabolic disorder. It is associated with serious life-threatening complications if not properly managed. The current study aimed at investigating the possible protective role of propolis on streptozotocin-induced diabetic nephropathy. A diabetic rat model was induced by a single intraperitoneal injection of 55 mg/kg streptozotocin. After 4 days, the diabetic rats received oral propolis (300 mg/kg/day) via gastric gavage for 28 days. Biochemical, histopathological and ultrastructural evaluations were performed. The results showed that: streptozotocin-induced diabetes was associated with a marked decrease in the serum high-density lipoproteins and antioxidant enzymes. However, a significant elevation in the levels of serum creatinine, blood urea nitrogen, uric acid, cholesterol, triglycerides and low-density lipoproteins was detected. Furthermore, streptozotocin treatment induced histopathological alterations of the renal cortex; in the form of distorted glomerular capillaries, widened Bowman's space and signs of epithelial tubular degeneration. Ultra-structurally, thickening and irregularity of the glomerular basement membrane and podocytes foot processes effacement were observed. The tubular epithelial cells showed swollen vacuolated mitochondria, scarce basal infoldings and loss of microvilli. Conversely, propolis partially restored the normal lipid profile, antioxidant biomarkers and renal cortical morphology. Propolis exhibited a sort of renoprotection through hypoglycemic, anti-hyperlipidemic and antioxidant effects.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of the combination of temozolomide and Eribulin on T98G human glioblastoma cell line: an ultrastructural study. 替莫唑胺和伊瑞布林联合用药对 T98G 人胶质母细胞瘤细胞系的影响：超微结构研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-06-25 DOI: 10.1080/01913123.2024.2371821

Gamze Tanriverdi, Belisa Kaleci, Furkan Yavuz, Hakan Sahin, Merjem Purelku, Zeliha Yazici, Sibel Kokturk

{"title":"The effects of the combination of temozolomide and Eribulin on T98G human glioblastoma cell line: an ultrastructural study.","authors":"Gamze Tanriverdi, Belisa Kaleci, Furkan Yavuz, Hakan Sahin, Merjem Purelku, Zeliha Yazici, Sibel Kokturk","doi":"10.1080/01913123.2024.2371821","DOIUrl":"10.1080/01913123.2024.2371821","url":null,"abstract":"Glioblastoma tumors are the most aggressive primary brain tumors that develop resistance to temozolomide (TMZ). Eribulin (ERB) exhibits a unique mechanism of action by inhibiting microtubule dynamics during the G2/M cell cycle phase. We utilized the T98G human glioma cell line to investigate the effects of ERB and TMZ, both individually and in combination. The experimental groups were established as follows: control, E5 (5 nM ERB), T0.75 (0.75 mM TMZ), T1 (1.0 mM TMZ), and combination groups (E5+T0.75 and E5+T1). All groups showed a significant decrease in cell proliferation. Apoptotic markers revealed a time-dependent increase in annexin-V expression, across all treatment groups at the 48-hour time point. Caspase-3, exhibited an increase in the combination treatment groups at the 48-hour mark. Transmission electron microscopy (TEM) revealed normal ultrastructural features in the glioma cells of the control group. However, treatments induced ultrastructural changes within the spheroid glioblastoma model, particularly in the combination groups. These changes included a dose-dependent increase in autophagic vacuoles and apoptotic morphology of the cells. In conclusion, the similarity in the mechanism of action between ERB and TMZ suggests the potential for synergistic effects when combined. Our results highlight that this combination induced severe damage and autophagy in glioma spheroids after 48 hours.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141447154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of gallic acid on tebuconazole-induced adverse effects in the cerebellum of adult albino rats: histopathological and immunohistochemical evidence. 没食子酸对戊唑醇诱导的成年白化大鼠小脑不良反应的改善作用：组织病理学和免疫组化证据。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI: 10.1080/01913123.2024.2387685

Omnia I Ismail, Hala Mohamed Hassanin

{"title":"Ameliorative effects of gallic acid on tebuconazole-induced adverse effects in the cerebellum of adult albino rats: histopathological and immunohistochemical evidence.","authors":"Omnia I Ismail, Hala Mohamed Hassanin","doi":"10.1080/01913123.2024.2387685","DOIUrl":"10.1080/01913123.2024.2387685","url":null,"abstract":"Tebuconazole (TEB) is a common triazole sterol demethylation inhibitor fungicide utilized to manage a variety of diseases in crops like cereals, fruits, and vegetables. The aim of this work was to assess the effects of TEB on the structure of the cerebellum in adult albino rats and possible protective impact of co-administration of Gallic acid (GA). Four groups of forty adult male albino rats were randomly selected, and the rats in group I received corn oil through daily gavage for 4 weeks. Group II received GA dissolved in the normal saline at a dose of 100 mg/kg through daily gavage for 4 weeks, group III administered with TEB dissolved in corn oil at its acceptable daily intake dose (0.02 mg/kg body weight) through daily gavage for 4 weeks, group IV rats received both TEB and GA. For light microscopic, ultrastructural, and immunohistochemical investigations, cerebellar specimens were prepared. TEB exposure led to neuronal damage in the form of degenerated Purkinje cells with vacuolated cytoplasm, areas of lost Purkinje cells, the basket cells appeared vacuolated with degenerated neuropil, the granule cells clumped with congested areas between them, dilated cerebellar islands, weak positive bcl2 immunoreactions in the Purkinje cells, and numerous GFAP-positive astrocytes. GA mitigated TEB-mediated histological changes in the cerebellar cortex. We concluded that TEB caused Purkinje neurons in the rat cerebellar cortex to degenerate and undergo apoptosis. GA had a neuroprotective benefit against TEB toxicity in the rat cerebellar cortex.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features that characterize monoclonal light chain ("myeloma") cast nephropathy with immunofluorescence challenges and emphasis on electron microscopy. 单克隆轻链（"骨髓瘤"）铸型肾病的特征与免疫荧光挑战，重点是电子显微镜。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-21 DOI: 10.1080/01913123.2024.2390892

Guillermo A Herrera, Luan D Truong, Sadhna Dhingra, Elba A Turbat-Herrera

{"title":"Features that characterize monoclonal light chain (\"myeloma\") cast nephropathy with immunofluorescence challenges and emphasis on electron microscopy.","authors":"Guillermo A Herrera, Luan D Truong, Sadhna Dhingra, Elba A Turbat-Herrera","doi":"10.1080/01913123.2024.2390892","DOIUrl":"10.1080/01913123.2024.2390892","url":null,"abstract":"Renal disease is a common cause of morbidity and mortality in patients with plasma cell dyscrasias. The serum-free light chain assay is used in patients, mostly older, with unexplained acute kidney injury to screen for potential myeloma cast nephropathy. This study consists of a systematic review of diagnostic features in myeloma cast nephropathy. The morphological features of tubular casts in patients with multiple myeloma have not been systematically analyzed. This study focuses on the morphology of these casts, emphasizing ultrastructural features, in a series of 23 patients with light chain (\"myeloma\") cast nephropathy and compared them with casts in 10 patients with various diseases. The immunofluorescence data were correlated with morphological findings to provide diagnostic assessments and practice guidelines. The ultrastructural features identified as diagnostic of casts associated with myeloma included: amyloid and crystals in the casts, multiple well-defined fracture planes forming a complex jigsaw puzzle arrangement of cast contents, indicative of the fragility of the immunoglobulin light chains involved, and reactive tubular cells lining the tubules with the casts. These features were seen in 95.2% of MCN cases and none of the casts in other renal conditions. Myeloma casts exhibited light chain monoclonality in a significant percentage of the MCN cases and often no staining for IgA or IgM. In contrast, the majority of non-myeloma casts stained for both kappa and lambda light chains, lgA, and lgM, and showed ultrastructurally a rather uniform finely to coarsely granular electron density occasionally admixed with cellular debris.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excessive lipid production shapes glioma tumor microenvironment. 过多的脂质生成塑造了胶质瘤的肿瘤微环境。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-19 DOI: 10.1080/01913123.2024.2392728

Haitham H Maraqah, John Paul Aboubechara, Mones S Abu-Asab, Han Sung Lee, Orwa Aboud

{"title":"Excessive lipid production shapes glioma tumor microenvironment.","authors":"Haitham H Maraqah, John Paul Aboubechara, Mones S Abu-Asab, Han Sung Lee, Orwa Aboud","doi":"10.1080/01913123.2024.2392728","DOIUrl":"10.1080/01913123.2024.2392728","url":null,"abstract":"Disrupted lipid metabolism is a characteristic of gliomas. This study utilizes an ultrastructural approach to characterize the prevalence and distribution of lipids within gliomas. This study made use of tissue from IDH1 wild type (IDH1-wt) glioblastoma (n = 18) and IDH1 mutant (IDH1-mt) astrocytoma (n = 12) tumors. We uncover a prevalent and intriguing surplus of lipids. The bulk of the lipids manifested as sizable cytoplasmic inclusions and extracellular deposits in the tumor microenvironment (TME); in some tumors the lipids were stored in the classical membraneless spheroidal lipid droplets (LDs). Frequently, lipids accumulated inside mitochondria, suggesting possible dysfunction of the beta-oxidation pathway. Additionally, the tumor vasculature have lipid deposits in their lumen and vessel walls; this lipid could have shifted in from the tumor microenvironment or have been produced by the vessel-invading tumor cells. Lipid excess in gliomas stems from disrupted beta-oxidation and dysfunctional oxidative phosphorylation pathways. The implications of this lipid-driven environment include structural support for the tumor cells and protection against immune responses, non-lipophilic drugs, and free radicals.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emperipolesis in pleural fluid mesothelial cells. A phenomenon not associated with Rosai-Dorfman disease, report of a case. 胸腔积液间皮细胞中的包膜。一个与罗赛-多夫曼病无关的现象，一个病例的报告。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-07-30 DOI: 10.1080/01913123.2024.2382987

Diana Sofía Vargas-Lías, Mónica Lizette Serrano-Arévalo, Lara-Torres César, Corredor-Alonso Guillermo Ernesto, Hugo R Dominguez-Malagon

引用次数: 0

The possible protective effect of ginger extract on toxic changes induced by bisphenol A on the thyroid gland of adult male albino rats: light and electron microscopic study. 生姜提取物对双酚 A 诱导的成年雄性白化大鼠甲状腺毒性变化的可能保护作用：光镜和电子显微镜研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-08-27 DOI: 10.1080/01913123.2024.2395849

Sara Mohammed Taha Denewer, Safinaz Moustafa Mahmoud Ali, Nawal Awad Hasanin, Dalia Refat El-Bassouny

{"title":"The possible protective effect of ginger extract on toxic changes induced by bisphenol A on the thyroid gland of adult male albino rats: light and electron microscopic study.","authors":"Sara Mohammed Taha Denewer, Safinaz Moustafa Mahmoud Ali, Nawal Awad Hasanin, Dalia Refat El-Bassouny","doi":"10.1080/01913123.2024.2395849","DOIUrl":"https://doi.org/10.1080/01913123.2024.2395849","url":null,"abstract":"Bisphenol A (BPA) is a chemical substance used in the plastic industry and considered as an endocrine disruptor. Ginger is a herbal material used in the food industry and has antioxidant activity. The present study was performed to evaluate the histological changes in the thyroid gland of adult male albino rats after intake of BPA and if there is any protective role for ginger extract (GE). Eighty adult male rats were divided equally into four groups. Group I as a control group, group II included rats that received 250 mg/kg/day GE orally for eight weeks, group III included rats that received 200 mg/kg/day BPA orally for the same period and group IV included rats that received BPA in the same dose for the same duration concomitantly with GE. At the end of the experiment, blood samples were taken for hormonal essay and tissue samples were processed. Light and electron microscopic studies were done. Morphometric and statistical studies were carried out. Group III showed degenerative changes in the thyroid gland, decreased serum levels of T3 and T4 and a strong positive inducible nitric oxide synthase (iNOS) immune response. Group IV showed restoration of thyroid gland architecture and function. In conclusion, GE protected the thyroid structure from the damaging effect of BPA oxidative stress through its anti-oxidant effect, thus preserving thyroid activity.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and histopathological changes of maternal levetiracetam on the cerebellar cortex of the offspring of albino rat. 母体左乙拉西坦对白化大鼠后代小脑皮层的形态学和组织病理学变化

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-07-03 Epub Date: 2024-06-08 DOI: 10.1080/01913123.2024.2353064

Safaa M Hanafy

{"title":"Morphological and histopathological changes of maternal levetiracetam on the cerebellar cortex of the offspring of albino rat.","authors":"Safaa M Hanafy","doi":"10.1080/01913123.2024.2353064","DOIUrl":"10.1080/01913123.2024.2353064","url":null,"abstract":"Levetiracetam (LEV) is being used by women with reproductive-age epilepsy at a significantly higher rate. The purpose of the study was to assess how levetiracetam treatment during pregnancy affected the offspring's weight and cerebellum. Forty pregnant rats were divided into two groups (I, II). Two smaller groups (A, B) were created from each group. The rats in group I were gavaged with approximately 1.5 mL/day of distilled water either continuously during pregnancy (for subgroup IA) or continuously during pregnancy and 14 days postpartum (for subgroup IB). The rats in group II were gavaged with about 1.5 mL/day of distilled water (containing 36 mg levetiracetam) either continuously during pregnancy (for subgroup IA) or continuously during pregnancy and 14 days postpartum (for subgroup IB). After the work was completed, the body weight of the pups in each group was recorded, and their cerebella were analyzed histologically and morphometrically. Following levetiracetam treatment, the offspring showed decreased body weight and their cerebella displayed delayed development and pathological alterations. These alterations manifested as, differences in the thicknesses of the layers of cerebellar cortex as compared to the control groups; additionally, their cells displayed cytoplasmic vacuolation, nuclear alterations, fragmented rough endoplasmic reticulum and lost mitochondrial cristae. Giving levetiracetam to pregnant and lactating female rats had a negative impact on the body weight and cerebella of the offspring. Levetiracetam should be given with caution during pregnancy and lactation.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0