Ultrastructural Pathology最新文献

{"title":"Understanding glioma pathogenesis through ultrastructural analysis.","authors":"Muhammad Sulman, John Paul Aboubechara, Mones S Abu-Asab, Orwa Aboud","doi":"10.1080/01913123.2025.2558625","DOIUrl":"https://doi.org/10.1080/01913123.2025.2558625","url":null,"abstract":"<p><p>Gliomas arise from glial cells and make up to 85% of malignant brain tumors, with an average survival of only 15 months for glioblastoma. Advances in our understanding of glioma pathogenesis and treatment require a critical analysis of the tumor ultrastructure and its microenvironment. Ultrastructural analysis (UA) using high-resolution electron microscopy enables crucial insights into the cellular and subcellular characteristics of gliomas, revealing organelle abnormalities and confirming molecular alterations. We focus on recent developments in our understanding of the ultrastructural pathology of gliomas that show the abundance of lipids found in both intracellular and extracellular environments. These lipid changes are associated with aberrant structures of mitochondria and even the disappearance of cellular membranes. Significant disruptions were also noted in tumors' blood vessels and intriguing details emerged about neovascularization and the atypical content and structure of vessel walls. In a typical display of mimicry, tumor cells replaced endothelial cells on the luminal wall of the vessels. Recent UA findings also detected multinucleated giant cells in high prevalence with the presence of extrachromosomal DNA in the microenvironment. Finally, we provide an overview of the implications of UA findings regarding future research, diagnostic modalities, and therapeutic options.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":1.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural analysis of mitochondria-associated membranes in lymphocytes of newly diagnosed type 2 diabetes mellitus.","authors":"Tamara Martinovic, Aleksa Zivkovic, Darko Ciric, Katarina Lalic, Iva Rasulic, Danica Djuricic, Ana Júlia Bonine de Melo, Andjelija Stankovic, Vladimir Bumbasirevic, Tamara Kravic-Stevovic","doi":"10.1080/01913123.2025.2550634","DOIUrl":"https://doi.org/10.1080/01913123.2025.2550634","url":null,"abstract":"<p><p>There is a growing number of evidence that mitochondrial dysfunction plays an important role in pathogenesis of diabetes mellitus. At near-contact sites between mitochondria and the ER, membranes of these organelles are juxtaposed within distance of 50 nm, referred to as mitochondria-associated membranes (MAMs). The aim of this study was to carry out an ultrastructural analysis of mitochondria-associated membranes in peripheral blood lymphocytes of patients with newly discovered type 2 diabetes mellitus and compare them with the same structures in lymphocytes of healthy persons. Transmission electron microscopy was employed to study peripheral blood lymphocytes from newly diagnosed patients not undergoing any metabolic therapy or antidiabetic medications, as well as from healthy persons. Lymphocytes of diabetic patients had more mitochondria, albeit with unchanged mitochondrial fractional volume, and more MAMs compared to lymphocytes from healthy individuals. An increase in the number of mitochondria and MAMs in peripheral blood lymphocytes could reflect disturbed mitochondrial dynamics and immunological changes that are present in patients with type 2 diabetes mellitus. In conclusion, the results obtained in our study emphasize the possible role of mitochondria and mitochondria-associated membranes in the metabolic changes of type 2 diabetes mellitus.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":1.2,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dimorphism of the multinucleated giant cells of gliomas.","authors":"Muhi Dean Barazi, John Paul Aboubechara, Muhammad Sulman, Haitham H Maraqah, Mones S Abu-Asab, Han Sung Lee, Orwa Aboud","doi":"10.1080/01913123.2025.2551738","DOIUrl":"10.1080/01913123.2025.2551738","url":null,"abstract":"<p><p>Extensive research has begun to uncover the molecular characteristics of high grade gliomas. However, an ultrastructural understanding of their pathogenesis remains largely unexplored. Multinucleated giant cells are large cells with multiple nuclei thought to form from the fusion of multiple neoplastic cells. In this study, we aim to elucidate the nature of the multinucleated giant cells (MGCs) within <i>IDH1</i>-wild type glioblastoma (GBM) and <i>IDH1</i>-mutant astrocytoma, grade 4, by characterizing their phenotypes, ontogenies, morphologies, prevalence, significance, and potential impact on tumor progression and treatment resistance. Utilizing transmission electron microscopy (TEM), we examined 30 tumors (18 <i>IDH1</i>-wild type GBMs and 12 <i>IDH1</i>-mutant astrocytomas) and found that they share two types of MGCs. Type 1 is formed by the fusion of several tumor cells. Type 2 seems to be produced by tumor fibrillar cells filled with intermediate filaments (IF) and lipids through two processes, either by cell fusion or by the immigration of naked nuclei to a larger IF-filled tumor cell. Our results showed that MGCs are abundantly present in 43% of cases, making them less rare than previously believed. The two MGC types occurred solely or in combination in both types of gliomas. Furthermore, MGCs appear non-proliferative; and therefore, their contribution to tumorigenesis and proliferation is not yet fully resolved.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":1.2,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of DNA damage and growth arrest by citalopram in breast cancer cells mediated via activation of Gadd45a and apoptotic genes.","authors":"Mohammed Salama, Ahmed Elamin, Magda Youssif, Noura A Mattar","doi":"10.1080/01913123.2025.2454691","DOIUrl":"10.1080/01913123.2025.2454691","url":null,"abstract":"<p><p>Breast cancer patients experience more severe emotional distress and depression compared to those with other cancers. Selective serotonin reuptake inhibitors (SSRIs), like citalopram, are commonly used to treat depression. However, the link between SSRI use and breast cancer progression is debated. This study examined the cytotoxic effects of citalopram on triple-negative (MDA-MB231) and ER-positive (MCF-7) breast cancer cells. Results showed a significant decrease in cell viability in both cell lines following citalopram treatment. Interestingly, flow cytometry analysis revealed increased apoptotic cells and induction of cell cycle arrest upon treatment of the cells with citalopram. MCF-7 cells were arrested in the sub-G0-G1 phase, while MDA-MB231 cells accumulated in the S phase. Gene expression analysis demonstrated increased Bax expression and decreased Bcl2 levels. Moreover, cytochrome c and NF-κB were upregulated upon treatment with citalopram. Furthermore, transmission electron microscopy (TEM) analysis of treated cells showed apoptotic morphological changes including shrunken nuclei, membrane blebbing, and chromatin condensation with prominent appearance of autophagosomes and autolysosomes. Additionally, GADD45a and p21, involved in growth arrest and DNA damage, were significantly upregulated. In conclusion, citalopram's ability to induce apoptosis and alter cell cycle suggests its potential in breast cancer treatment.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"158-169"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin II promotes intramural hematoma of aorta in juvenile mice at early stage.","authors":"Weiliang Sun, Changan Yu, Jing Guo, Huina Wang, Shurui Dou, Yuting Zhang, Jingang Zheng, Yanxiang Gao","doi":"10.1080/01913123.2025.2474447","DOIUrl":"10.1080/01913123.2025.2474447","url":null,"abstract":"<p><strong>Objectives: </strong>Intramural hematoma (IMH) is a serious aortic condition characterized by the presence of a contained hematoma within the aortic media. However, the animal model with a high incidence of IMH was lacking, and the specific pathological characteristics of IMH have not been thoroughly characterized.</p><p><strong>Methods and results: </strong>We conducted an experimental study using 4-week-old male, 4-week-old female, and 8-week-old male C57BL/6J mice. These mice were subjected to angiotensin II infusion at a rate of 1000 ng/kg/min for a period of 4 days. In situ imaging was performed, and aorta was harvested and serially sectioned. Histological staining and immunostaining techniques were employed, and the subcellular structure was examined using transmission electron microscopy. Our findings revealed that 4-week-old male mice exhibited a higher susceptibility to angiotensin II-induced IMH, characterized by more circumferential appearances and larger affected areas. Furthermore, IMH was more likely to occur in the upper segment of the descending aorta rather than the lower segment. Within the IMH, older fibrinous thrombus was predominantly observed near the adventitia, while younger red thrombus was more prevalent near the lumen. Additionally, platelet activation and degranulation were observed, along with fibrin cross-linking and thrombus organization, indicating a potential relationship between platelet activation and the progression of IMH.</p><p><strong>Conclusion: </strong>Our study demonstrated that angiotensin II infusion promoted the development of IMH during the early stages, especially in juvenile mice. Furthermore, the presence of platelet activation and thrombus organization suggested their potential involvement in the progression of IMH.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"148-157"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astrocyte alterations during Osmotic Demyelination Syndrome: intermediate filaments, aggresomes, proteasomes, and glycogen storages.","authors":"Jacques Gilloteaux, Corry Charlier, Valérie Suain, Charles Nicaise","doi":"10.1080/01913123.2025.2468700","DOIUrl":"10.1080/01913123.2025.2468700","url":null,"abstract":"<p><strong>Introduction: </strong>A murine model mimicking the human osmotic demyelination syndrome (ODS) revealed with histology demyelinated alterations in the relay posterolateral (VPL) and ventral posteromedial (VPM) thalamic nuclei 12 h and 48 h after chronic hyponatremia due to a fast reinstatement of osmolality. Abnormal expression astrocyte markers ALDHL1 and GFAP with immunohistochemistry in these ODS altered zones, prompted aims to verify in both protoplasmic and fibrillar astrocytes with ultrastructure those changes and other associated subcellular modifications.</p><p><strong>Method: </strong>This ODS investigation included four groups of mice: Sham (NN; <i>n</i> = 13), hyponatremic (HN; <i>n</i> = 11), those sacrificed 12 h after a fast restoration of normal natremia (ODS12h; <i>n</i> = 6), and mice sacrificed 48 h afterward, or ODS48 h (<i>n</i> = 9). Out of those four groups of mice, with LM and ultrastructure microscopy, the thalamic zones included NN (<i>n</i> = 2), HN (<i>n</i> = 2), ODS12h (<i>n</i> = 3) and ODS48h (<i>n</i> = 3) samples. There, comparisons between astrocytes included organelles, GFAP, and glycogen content changes.</p><p><strong>Results: </strong>Thalamic ODS epicenter damages comprised both protoplasmic (PA) and fibrillar (FA) astrocyte necroses along with those of neuropil destructions and neuron Wallerian demyelinated injuries surrounded by a centrifugal region gradient revealing worse to mild destructions. Ultrastructure aspects of resilient HN and ODS12h PAs disclosed altered mitochondria and accumulations of beta- to alpha-glycogen granules that became eventually captured into phagophores as glycophagosomes in ODS48h. HN and ODS12h time lapse FAs accumulated ribonucleoproteins, cytoskeletal aggresomes, and proteasomes but distant and resilient ODS48h FAs maintained GFAP fibrils along with typical mitochondria and dispersed β-glycogen, including in their neuropil surroundings. Thus, ODS triggered astrocyte injuries that involved both post-transcriptional and post-translational modifications such that astrocytes were unable to use glycogen and metabolites due to their own mitochondria defects while accumulated stalled ribonucleoproteins, cytoskeletal aggresomes were associated with proteasomes and GFAP ablation. Resilient but distant astrocytes revealed restitution of amphibolism where typical carbohydrate storages were revealed along with GFAP, as tripartite extensions supply for restored nerve axon initial segments, neural Ranvier's junctions, and oligodendrocyte -neuron junctional contacts.</p><p><strong>Conclusion: </strong>ODS caused astrocyte damage associated with adjacent neuropil destruction that included a regional demyelination caused by a loss of dispatched energetic and metabolic exchanges within the injured region, bearing proportional and collateral centrifugal injuries, which involved reactive repairs time after rebalanced osmolarity.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"170-215"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterozygous missense mutation of the fibrinogen gene associated with cryptogenic liver disease in a 15-months-old Canadian caucasian child.","authors":"Mohit Kehar, Robert J Klaassen, Consolato M Sergi","doi":"10.1080/01913123.2024.2447853","DOIUrl":"10.1080/01913123.2024.2447853","url":null,"abstract":"<p><p>Hepatic fibrinogen storage disease is an uncommon autosomal dominant hereditary illness marked by hypofibrinogenemia and the accumulation of variant fibrinogen in the hepatic endoplasmic reticulum. We present an asymptomatic 15-month-old male with elevated liver enzymes. Test results indicate hypofibrinogenemia. The liver biopsy revealed circular eosinophilic inclusion bodies within the hepatocyte cytoplasm. After diastase pretreatment, the inclusion bodies did not stain using the periodic acid - Schiff procedure. Ultrastructural examination revealed the characteristic fibrinogen storage curvilinear inclusions. Sequence analysis using the Blueprint Genetics (BpG) FLEX Bleeding Disorder/Coagulopathy Panel identified a heterozygous missense variant <i>FGG</i> c.1075 G>C, p. (Gly359Arg). Thus, the patient was diagnosed with hepatic fibrinogen storage disease. Our findings suggest that in patients with asymptomatic elevated liver enzymes presenting with unanticipated hypofibrinogenemia, hepatic fibrinogen storage disorder must be included in the differential diagnosis. Furthermore, our results underscore the significance of molecular diagnosis in patients diagnosed with cryptogenic liver disease.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"235-242"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ultrastructural changes in the adult rat ovary after administration of copper oxide nanoparticles and the possible ameliorative influence of selenium.","authors":"Abeer Mohamed Ali Shalaby, Eman Shaaban Abdel-Aziz Abul-Ela, Amal Mohamed Moustafa, Shehab Hafez Mohamed","doi":"10.1080/01913123.2024.2449091","DOIUrl":"10.1080/01913123.2024.2449091","url":null,"abstract":"<p><p>There is an important concern about the potential health and environmental risks that may develop due to exposure to copper oxide nanoparticles (CuO-NPs). Selenium is an essential trace element. It supports the expression of a variety of selenoproteins. The present study was designed to study the ultrastructural and biochemical changes in the adult rat ovary after oral administration of CuO-NPs and to assess the possible ameliorative influence of Selenium. Sixty adult female albino rats were divided in two major groups: Group I and Group II. Group I was further subdivided into three groups: Group IA (control), Group IB: received a single high dose of 2000 mg/kg CuO-NPs, Group IC: received selenium (0.5 mg/kg), five days before giving a single high dose of CuO-NPs (2000 mg/kg). Thereafter, given selenium for 14 days. Group II was subdivided into three groups: Group IIA (control), Group IIB: received a small dose of 300 mg/kg of CuO-NPs for 28 days, Group IIC: received selenium (0.5 mg/kg), five days before starting concomitant administration of CuO-NPs (300 mg/kg) and Selenium (0.5 mg/kg) for 28 days. Damage of the ovarian ultrastructural features, increased MDA levels, and decreased serum estrogen and progesterone hormones levels were detected in group IB and group IIB. Group IC and group IIC showed improvement of ovarian ultrastructural, decreased MDA levels, and increased serum estrogen and progesterone hormones levels as compared to group IB and group IIB indicating that Selenium could decrease the damage induced by CuO-NPs in the adult rat ovaries.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"109-129"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of copper oxide nanoparticles (CuONPs) on the testes of adult male albino rats and the possible protective role of extra virgin olive oil (EVOO).","authors":"Amany F Mohamed, Safaa M Hanafy, Ranya Mohammed Abdelgalil, Amany M Abo-Ouf","doi":"10.1080/01913123.2025.2462534","DOIUrl":"10.1080/01913123.2025.2462534","url":null,"abstract":"<p><p>We have assessed the effects of copper oxide nanoparticles on the testis of adult male albino rats, and evaluated the protective potential of EVOO, which has antioxidant properties. The study involved treatment of seventy adult male rats followed by examination of their testis. The rats were divided into four groups (I-V), each contained 20 rats except group II which contained 10 rats. Each of groups (I, III, IV) was subdivided equally into two subgroups (A and B). Rats in group I did not receive any treatment (IA) or injected intraperitoneal (IP) with 0.5 ml of distilled water daily for two weeks (IB). Rats in group II were gavaged 0.4 ml EVOO daily for 2 weeks. Rats in group III injected IP daily for 2 weeks with 0.5 ml distilled water containing 1 mg CuO NPs (subgroup IIIA) and 4 mg CuO NPs (IIIB). Rats in group IV were gavaged 0.4 ml EVOO before IP injected daily for 2 weeks with 0.5 ml distilled water containing either 1 mg CuO NPs (subgroup IVA) or 4 mg CuO NPs (IVB). After treatment, morphological, histological and biochemical studies on the testes were conducted. Examination of CuO NPs treated groups revealed dose dependant increase in pathological changes. These changes were reduced body weight, distorted basement membranes of seminiferous tubules and degeneration of seminiferous cells. Co-administration of EVOO ameliorated most pathological changes. We concluded that CuO NPs induced deteriorating changes in rats' testes which were improved after co-administration of EVOO.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"130-147"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying gene expression and cellular pathways involved in glomerular AL-amyloidosis and correlation with experimental data: seeking novel therapeutic interventions.","authors":"Guillermo A Herrera, Jiamin Teng, Chun Zeng, Luis Del Pozo-Yauner, Bing Liu, Elba A Turbat-Herrera","doi":"10.1080/01913123.2025.2468708","DOIUrl":"10.1080/01913123.2025.2468708","url":null,"abstract":"<p><p>The prognosis of myeloma is based on controlling the plasma cell burden and thus, management of the production of monoclonal light chains has improved considerably, expanding survival and quality of life. However, the effects of the monoclonal light chains in the various organs result in alterations that may lead to renal failure. There is a crucial need to ameliorate or abolish renal damage. Organ-based therapies must be developed. Glomerulopathic light chains interact with mesangial cells using the SORL1 receptor and downstream effects lead to divergent mesangial alterations. While the multi-step process occurring when amyloidogenic light chains interact with mesangial cells has been elucidated in the laboratory, gene expression profiles and activated cellular pathways in human glomeruli have not been probed. Mesangial cells from five renal biopsies at different stages of glomerular amyloidosis were interrogated using spatial transcriptomics and compared with those from normal biopsy controls to identify cellular pathways and gene expression changes. The two most significant statistically overexpressed genes (FDR <0.05) when comparing control, early vs late cases were heat shock protein 90AB1 and HSPB1, known to be involved in protein misfolding and aggregation. The overexpressed genes exercise function and regulation over cellular pathways promoting apoptosis, vesicular transport, metalloproteinase activation, collagen degradation, gap junction degradation, GTPase cycle activation, and organelle biogenesis. This data confirmed the results previously reached in the research laboratory. Spatial transcriptomics demonstrated uniquely activated genes and cellular pathways in mesangial cells involved in the initiation and progression of glomerular amyloidosis, uncovering novel genes and new therapeutic targets.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"216-234"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}