Ultrastructural Pathology最新文献

{"title":"Histological and biochemical evaluation of the effect of nandrolone decanoate on the prostate in postpubertal albino rats.","authors":"Heba M Abdel-Aziz, Amal S Abdel-Salam, Fayza E Ahmed, Abeer A Abdelrahman, Maha Z Mohammed","doi":"10.1080/01913123.2025.2531779","DOIUrl":"https://doi.org/10.1080/01913123.2025.2531779","url":null,"abstract":"<p><p>Nandrolone decanoate, a synthetic form of the testosterone hormone, is misused by some bodybuilders and adolescents to gain muscle mass. We investigated its effect on the prostate during postpubertal age and evaluated the reversibility of these effects after a period of cessation. Thirty albino rats were utilized and categorized into three distinct groups: Control group, a treated group that received nandrolone decanoate weekly by intramuscular injection with 10 mg/kg for 4 weeks while recovery group received nandrolone, similar to the treated group, followed by an additional recovery period of 4 weeks. In the treated group, there were significant elevation in IL-1β, IL-18, IL-6, TNF-α, COX2, MDA, BAX, PSA, and miRNA-155 expression and decreased in SOD, GST, bcl2, and testosterone hormone compared to the control group. Prostatic acini showed marked epithelial stratification, weak PAS positive reactions, excess collagen depositions, and negative ARs nuclear immunoexpression. Ultrastructrally, cytoplasm contained vacuoles and dilated cisterna of RER. In the recovery group, insignificant differences were observed between the recovery and control groups, and most of the acini appeared normal except for minimal changes. Nandrolone-induced structural and functional impairment of the prostate at cellular and molecular basis and cessation of it in the recovery group ameliorated the impairment.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-15"},"PeriodicalIF":1.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light chain crystalline podocytopathy caused by electron-lucent crystals - a diagnostic challenge: a case report.","authors":"Rajib K Gupta, Maria E Pagtalunan, Ann M Wexler, Joseph M Tuscano","doi":"10.1080/01913123.2025.2535622","DOIUrl":"10.1080/01913123.2025.2535622","url":null,"abstract":"<p><p>We report here a challenging diagnosis of light chain crystalline podocytopathy in a patient with sub-nephrotic proteinuria, microscopic hematuria, glucosuria and elevated free kappa light chains but without any initial clinical evidence of frank multiple myeloma. We also discuss the diagnostic challenges of this case which include rather unique and seemingly innocuous light microscopic morphology of the glomeruli and the critical roles of paraffin immunofluorescence and need for electron microscopy to confirm the diagnosis. The kappa restriction of the crystals within the podocytes was confirmed only by paraffin immunofluorescence (it went undetected by routine immunofluorescence) and electron microscopy of the glomeruli showed the podocytopathy to be caused by electron-lucent crystals which is a rare variant of light chain crystals, with electron-dense crystals being the predominant variant of light chain crystals.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-6"},"PeriodicalIF":1.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aggressive angiomyxoma: an ultrastructural study update of five cases.","authors":"Abigail Martínez-Jerónimo, Jazmín Itzayana Salazar-Leal, Guillermo Ernesto Corredor-Alonso, Hugo Domínguez-Malagón","doi":"10.1080/01913123.2025.2508401","DOIUrl":"https://doi.org/10.1080/01913123.2025.2508401","url":null,"abstract":"<p><p>The aggressive angiomyxoma (AAM) is a rare mesenchymal tumor known for its locally aggressive behavior and high recurrence rate, predominantly affecting women. Through this analysis of five cases located in the vulva, scrotum, and perineum, the study employs histopathological, immunohistochemical, and ultrastructural techniques to elucidate the tumor's characteristics. Findings revealed confirmatory histopathological and immunohistochemical features. The ultrastructural study details the distinctive features of this entity showing characteristics compatible with myofibroblastic differentiation.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-8"},"PeriodicalIF":1.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcomatoid mesothelioma vs. myogenic sarcoma: a strong case for diagnostic electron microscopy: a case report.","authors":"Nadine H Oury, Katherine Killian D O, Tim D Oury","doi":"10.1080/01913123.2025.2505165","DOIUrl":"https://doi.org/10.1080/01913123.2025.2505165","url":null,"abstract":"<p><p>Mesothelioma is often considered a difficult diagnosis due to its rarity, the wide variety of histological patterns and the propensity of metastasis from cancers of unknown origin to serosal surfaces. The advent of numerous new immunochemical markers has provided extensive aid in diagnosing epithelial mesotheliomas. However, immunochemical markers that assist in the diagnosis of sarcomatoid mesothelioma remain limited. Sarcomatoid mesothelioma is the most aggressive and least common form of mesothelioma. Therefore, sarcomatoid mesothelioma diagnosis has the added challenge of increased rarity in addition to a lack of distinctive immunochemical features. Electron microscopy (EM) is a useful tool for visualizing the ultrastructural components of different tumors and has been utilized to identify distinctive features in the diagnosis of epithelial mesotheliomas. Utilization of EM in cases of sarcomatoid mesotheliomas has been limited due to a lack of diagnostic ultrastructural markers. However, EM can still be useful in evaluation of sarcomatoid tumors when sarcomatoid mesotheliomas are part of the differential diagnosis. Here, we present the case of an individual with suspected sarcomatoid mesothelioma and demonstrate the utility of EM in differentiating alternative sarcomatoid malignancies, namely a myogenic sarcoma, by identifying diagnostic ultrastructural components.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-5"},"PeriodicalIF":1.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of DNA damage and growth arrest by citalopram in breast cancer cells mediated via activation of Gadd45a and apoptotic genes.","authors":"Mohammed Salama, Ahmed Elamin, Magda Youssif, Noura A Mattar","doi":"10.1080/01913123.2025.2454691","DOIUrl":"10.1080/01913123.2025.2454691","url":null,"abstract":"<p><p>Breast cancer patients experience more severe emotional distress and depression compared to those with other cancers. Selective serotonin reuptake inhibitors (SSRIs), like citalopram, are commonly used to treat depression. However, the link between SSRI use and breast cancer progression is debated. This study examined the cytotoxic effects of citalopram on triple-negative (MDA-MB231) and ER-positive (MCF-7) breast cancer cells. Results showed a significant decrease in cell viability in both cell lines following citalopram treatment. Interestingly, flow cytometry analysis revealed increased apoptotic cells and induction of cell cycle arrest upon treatment of the cells with citalopram. MCF-7 cells were arrested in the sub-G0-G1 phase, while MDA-MB231 cells accumulated in the S phase. Gene expression analysis demonstrated increased Bax expression and decreased Bcl2 levels. Moreover, cytochrome c and NF-κB were upregulated upon treatment with citalopram. Furthermore, transmission electron microscopy (TEM) analysis of treated cells showed apoptotic morphological changes including shrunken nuclei, membrane blebbing, and chromatin condensation with prominent appearance of autophagosomes and autolysosomes. Additionally, GADD45a and p21, involved in growth arrest and DNA damage, were significantly upregulated. In conclusion, citalopram's ability to induce apoptosis and alter cell cycle suggests its potential in breast cancer treatment.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"158-169"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin II promotes intramural hematoma of aorta in juvenile mice at early stage.","authors":"Weiliang Sun, Changan Yu, Jing Guo, Huina Wang, Shurui Dou, Yuting Zhang, Jingang Zheng, Yanxiang Gao","doi":"10.1080/01913123.2025.2474447","DOIUrl":"10.1080/01913123.2025.2474447","url":null,"abstract":"<p><strong>Objectives: </strong>Intramural hematoma (IMH) is a serious aortic condition characterized by the presence of a contained hematoma within the aortic media. However, the animal model with a high incidence of IMH was lacking, and the specific pathological characteristics of IMH have not been thoroughly characterized.</p><p><strong>Methods and results: </strong>We conducted an experimental study using 4-week-old male, 4-week-old female, and 8-week-old male C57BL/6J mice. These mice were subjected to angiotensin II infusion at a rate of 1000 ng/kg/min for a period of 4 days. In situ imaging was performed, and aorta was harvested and serially sectioned. Histological staining and immunostaining techniques were employed, and the subcellular structure was examined using transmission electron microscopy. Our findings revealed that 4-week-old male mice exhibited a higher susceptibility to angiotensin II-induced IMH, characterized by more circumferential appearances and larger affected areas. Furthermore, IMH was more likely to occur in the upper segment of the descending aorta rather than the lower segment. Within the IMH, older fibrinous thrombus was predominantly observed near the adventitia, while younger red thrombus was more prevalent near the lumen. Additionally, platelet activation and degranulation were observed, along with fibrin cross-linking and thrombus organization, indicating a potential relationship between platelet activation and the progression of IMH.</p><p><strong>Conclusion: </strong>Our study demonstrated that angiotensin II infusion promoted the development of IMH during the early stages, especially in juvenile mice. Furthermore, the presence of platelet activation and thrombus organization suggested their potential involvement in the progression of IMH.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"148-157"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astrocyte alterations during Osmotic Demyelination Syndrome: intermediate filaments, aggresomes, proteasomes, and glycogen storages.","authors":"Jacques Gilloteaux, Corry Charlier, Valérie Suain, Charles Nicaise","doi":"10.1080/01913123.2025.2468700","DOIUrl":"10.1080/01913123.2025.2468700","url":null,"abstract":"<p><strong>Introduction: </strong>A murine model mimicking the human osmotic demyelination syndrome (ODS) revealed with histology demyelinated alterations in the relay posterolateral (VPL) and ventral posteromedial (VPM) thalamic nuclei 12 h and 48 h after chronic hyponatremia due to a fast reinstatement of osmolality. Abnormal expression astrocyte markers ALDHL1 and GFAP with immunohistochemistry in these ODS altered zones, prompted aims to verify in both protoplasmic and fibrillar astrocytes with ultrastructure those changes and other associated subcellular modifications.</p><p><strong>Method: </strong>This ODS investigation included four groups of mice: Sham (NN; <i>n</i> = 13), hyponatremic (HN; <i>n</i> = 11), those sacrificed 12 h after a fast restoration of normal natremia (ODS12h; <i>n</i> = 6), and mice sacrificed 48 h afterward, or ODS48 h (<i>n</i> = 9). Out of those four groups of mice, with LM and ultrastructure microscopy, the thalamic zones included NN (<i>n</i> = 2), HN (<i>n</i> = 2), ODS12h (<i>n</i> = 3) and ODS48h (<i>n</i> = 3) samples. There, comparisons between astrocytes included organelles, GFAP, and glycogen content changes.</p><p><strong>Results: </strong>Thalamic ODS epicenter damages comprised both protoplasmic (PA) and fibrillar (FA) astrocyte necroses along with those of neuropil destructions and neuron Wallerian demyelinated injuries surrounded by a centrifugal region gradient revealing worse to mild destructions. Ultrastructure aspects of resilient HN and ODS12h PAs disclosed altered mitochondria and accumulations of beta- to alpha-glycogen granules that became eventually captured into phagophores as glycophagosomes in ODS48h. HN and ODS12h time lapse FAs accumulated ribonucleoproteins, cytoskeletal aggresomes, and proteasomes but distant and resilient ODS48h FAs maintained GFAP fibrils along with typical mitochondria and dispersed β-glycogen, including in their neuropil surroundings. Thus, ODS triggered astrocyte injuries that involved both post-transcriptional and post-translational modifications such that astrocytes were unable to use glycogen and metabolites due to their own mitochondria defects while accumulated stalled ribonucleoproteins, cytoskeletal aggresomes were associated with proteasomes and GFAP ablation. Resilient but distant astrocytes revealed restitution of amphibolism where typical carbohydrate storages were revealed along with GFAP, as tripartite extensions supply for restored nerve axon initial segments, neural Ranvier's junctions, and oligodendrocyte -neuron junctional contacts.</p><p><strong>Conclusion: </strong>ODS caused astrocyte damage associated with adjacent neuropil destruction that included a regional demyelination caused by a loss of dispatched energetic and metabolic exchanges within the injured region, bearing proportional and collateral centrifugal injuries, which involved reactive repairs time after rebalanced osmolarity.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"170-215"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterozygous missense mutation of the fibrinogen gene associated with cryptogenic liver disease in a 15-months-old Canadian caucasian child.","authors":"Mohit Kehar, Robert J Klaassen, Consolato M Sergi","doi":"10.1080/01913123.2024.2447853","DOIUrl":"10.1080/01913123.2024.2447853","url":null,"abstract":"<p><p>Hepatic fibrinogen storage disease is an uncommon autosomal dominant hereditary illness marked by hypofibrinogenemia and the accumulation of variant fibrinogen in the hepatic endoplasmic reticulum. We present an asymptomatic 15-month-old male with elevated liver enzymes. Test results indicate hypofibrinogenemia. The liver biopsy revealed circular eosinophilic inclusion bodies within the hepatocyte cytoplasm. After diastase pretreatment, the inclusion bodies did not stain using the periodic acid - Schiff procedure. Ultrastructural examination revealed the characteristic fibrinogen storage curvilinear inclusions. Sequence analysis using the Blueprint Genetics (BpG) FLEX Bleeding Disorder/Coagulopathy Panel identified a heterozygous missense variant <i>FGG</i> c.1075 G>C, p. (Gly359Arg). Thus, the patient was diagnosed with hepatic fibrinogen storage disease. Our findings suggest that in patients with asymptomatic elevated liver enzymes presenting with unanticipated hypofibrinogenemia, hepatic fibrinogen storage disorder must be included in the differential diagnosis. Furthermore, our results underscore the significance of molecular diagnosis in patients diagnosed with cryptogenic liver disease.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"235-242"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ultrastructural changes in the adult rat ovary after administration of copper oxide nanoparticles and the possible ameliorative influence of selenium.","authors":"Abeer Mohamed Ali Shalaby, Eman Shaaban Abdel-Aziz Abul-Ela, Amal Mohamed Moustafa, Shehab Hafez Mohamed","doi":"10.1080/01913123.2024.2449091","DOIUrl":"10.1080/01913123.2024.2449091","url":null,"abstract":"<p><p>There is an important concern about the potential health and environmental risks that may develop due to exposure to copper oxide nanoparticles (CuO-NPs). Selenium is an essential trace element. It supports the expression of a variety of selenoproteins. The present study was designed to study the ultrastructural and biochemical changes in the adult rat ovary after oral administration of CuO-NPs and to assess the possible ameliorative influence of Selenium. Sixty adult female albino rats were divided in two major groups: Group I and Group II. Group I was further subdivided into three groups: Group IA (control), Group IB: received a single high dose of 2000 mg/kg CuO-NPs, Group IC: received selenium (0.5 mg/kg), five days before giving a single high dose of CuO-NPs (2000 mg/kg). Thereafter, given selenium for 14 days. Group II was subdivided into three groups: Group IIA (control), Group IIB: received a small dose of 300 mg/kg of CuO-NPs for 28 days, Group IIC: received selenium (0.5 mg/kg), five days before starting concomitant administration of CuO-NPs (300 mg/kg) and Selenium (0.5 mg/kg) for 28 days. Damage of the ovarian ultrastructural features, increased MDA levels, and decreased serum estrogen and progesterone hormones levels were detected in group IB and group IIB. Group IC and group IIC showed improvement of ovarian ultrastructural, decreased MDA levels, and increased serum estrogen and progesterone hormones levels as compared to group IB and group IIB indicating that Selenium could decrease the damage induced by CuO-NPs in the adult rat ovaries.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"109-129"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of copper oxide nanoparticles (CuONPs) on the testes of adult male albino rats and the possible protective role of extra virgin olive oil (EVOO).","authors":"Amany F Mohamed, Safaa M Hanafy, Ranya Mohammed Abdelgalil, Amany M Abo-Ouf","doi":"10.1080/01913123.2025.2462534","DOIUrl":"10.1080/01913123.2025.2462534","url":null,"abstract":"<p><p>We have assessed the effects of copper oxide nanoparticles on the testis of adult male albino rats, and evaluated the protective potential of EVOO, which has antioxidant properties. The study involved treatment of seventy adult male rats followed by examination of their testis. The rats were divided into four groups (I-V), each contained 20 rats except group II which contained 10 rats. Each of groups (I, III, IV) was subdivided equally into two subgroups (A and B). Rats in group I did not receive any treatment (IA) or injected intraperitoneal (IP) with 0.5 ml of distilled water daily for two weeks (IB). Rats in group II were gavaged 0.4 ml EVOO daily for 2 weeks. Rats in group III injected IP daily for 2 weeks with 0.5 ml distilled water containing 1 mg CuO NPs (subgroup IIIA) and 4 mg CuO NPs (IIIB). Rats in group IV were gavaged 0.4 ml EVOO before IP injected daily for 2 weeks with 0.5 ml distilled water containing either 1 mg CuO NPs (subgroup IVA) or 4 mg CuO NPs (IVB). After treatment, morphological, histological and biochemical studies on the testes were conducted. Examination of CuO NPs treated groups revealed dose dependant increase in pathological changes. These changes were reduced body weight, distorted basement membranes of seminiferous tubules and degeneration of seminiferous cells. Co-administration of EVOO ameliorated most pathological changes. We concluded that CuO NPs induced deteriorating changes in rats' testes which were improved after co-administration of EVOO.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"130-147"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}