Ultrastructural Pathology最新文献

{"title":"Investigating the effect of the heavy metals cadmium, chromium and lead, alone and in combination on an endothelial cell line.","authors":"L van Strijp,&nbsp;M Van Rooy,&nbsp;J Serem,&nbsp;C Basson,&nbsp;Hm Oberholzer","doi":"10.1080/01913123.2023.2189986","DOIUrl":"https://doi.org/10.1080/01913123.2023.2189986","url":null,"abstract":"<p><p>Heavy metals are natural elements characterized by their relatively large atomic mass as well as high density. It can be introduced into the ecosystem by the mining of heavy metals from deep within the earth's crust, thereby exposing the metals into air and water systems. Cigarette smoke is another source of heavy metal exposure and has been shown to have carcinogenic, toxic and genotoxic properties. Cadmium, lead, and chromium are the most abundant metals found in cigarette smoke. In response to tobacco smoke exposure, endothelial cells release inflammatory and pro-atherogenic cytokines that are linked to endothelial dysfunction. Endothelial dysfunction is directly related to the production of reactive oxygen species, leading to endothelial cell loss through necrosis and/or apoptosis. The current study aimed to investigate the effect of cadmium, lead, and chromium, alone and as part of metal mixtures, on endothelial cells. The EA.hy926 endothelial cell line was exposed to different concentrations of each of these metals and their combinations and analyzed using flow cytometric analyses with Annexin V. A clear trend was seen with the Pb + Cr as well as the triple combination group with the significant increase of early apoptotic cells. Scanning electron microscopy was used to study possible ultrastructural effects. Morphological changes observed with scanning electron microscopy included cell membrane damage and membrane blebbing at certain metal concentrations. In conclusion, the exposure of endothelial cells to cadmium, lead, and chromium, caused a disruption in cellular processes and morphology, possibly diminishing the protective ability of endothelial cells.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"205-218"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9766805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An adrenal cortical adenoma with neuroendocrine-type granules mimicking pheochromocytoma.","authors":"Leonardo Rossi,&nbsp;Carlo Enrico Ambrosini,&nbsp;Liborio Torregrossa,&nbsp;Maria Margherita de Santi,&nbsp;Raffaella Guazzo,&nbsp;Tommaso Simoncini,&nbsp;Alessandra Bacca,&nbsp;Benard Gjeloshi,&nbsp;Francesco Pignatelli,&nbsp;Mattia Iachini,&nbsp;Elisa Loguercio,&nbsp;Chiara Becucci,&nbsp;Gabriele Materazzi","doi":"10.1080/01913123.2023.2201314","DOIUrl":"https://doi.org/10.1080/01913123.2023.2201314","url":null,"abstract":"<p><p>Clinical and biochemical presentation of adrenal tumors may rarely conflict with their histologic features. In the present report, we describe a rare case of adrenal neoplasm clinically and biochemically labeled as pheochromocytoma which at histologic examination resulted adrenal cortical tumor. The neoplasm was examined with the electron microscope which revealed the presence of electron-dense neuroendocrine-type granules next to intracytoplasmic lipid droplets. The patient underwent laparoscopic left adrenalectomy which leads to normalization of 24 h urinary metanephrine and normetanephrine. This exceptional entity should be taken into consideration when the clinical and laboratory features conflict with the histological examination. The pathologist can clarify the mixed nature of the tumor by means of the identification of neuroendocrine granules at the electron microscope examination.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"236-240"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9391414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evening primrose oil attenuates oxidative stress, inflammation, fibrosis, apoptosis, and ultrastructural alterations induced by metanil yellow in the liver of rat: a histological, immunohistochemical, and biochemical study.","authors":"Amany Mohamed Shalaby,&nbsp;Rania H Shalaby,&nbsp;Mohamed Ali Alabiad,&nbsp;Doaa I Abdelrahman,&nbsp;Mohammed Alorini,&nbsp;Fatima A Jaber,&nbsp;Shaimaa Mohamed Abdelfattah Hassan","doi":"10.1080/01913123.2023.2189987","DOIUrl":"https://doi.org/10.1080/01913123.2023.2189987","url":null,"abstract":"<p><p>The food color metanil yellow (Myl) is hazardous to several body systems. Evening primrose oil (EPO) was reported to have anti-inflammatory and anti-oxidant properties. The present work investigated the impact of Myl on the hepatic structure and function of rats and evaluated the protective effect of EPO. Forty adult male rats were divided into four groups: control, EPO (5 g/kg/day), Myl (200 mg/kg/day), and EPO- Myl group. Myl significantly increased liver enzymes, advanced glycation end products (AGE), oxidative stress parameters, pro-inflammatory cytokines, nuclear factor kappa B (NF-κB), and inducible nitric oxide synthase (iNOS). Blood vessels in the liver were dilated and congested, with cellular infiltration around them and associated with fibrosis. The hepatocytes were vacuolated and had dark nuclei. The immunohistochemical expression of iNOS, glial fibrillary acidic protein (GFAP), and Bax was significantly elevated. Ultrastructurally, the hepatocytes showed lipid droplets, irregular condensed nuclei with widened perinuclear space, dilated rER, mitochondria with destructed cristae, and multiple vacuoles. Dilated congested blood sinusoids and collagen fiber bundles were seen between hepatocytes. Interestingly, these alterations were less pronounced in rats co-administrated with EPO and Myl. In conclusion, EPO can protect liver against the toxic effects of Myl due to its anti-inflammatory and anti-oxidant activities.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"188-204"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9403273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteinuria in thrombotic microangiopathy is associated with partial podocytopathy.","authors":"Megan Moore,&nbsp;Olabisi Afolayan-Oloye,&nbsp;Olaf Kroneman,&nbsp;Wei Li,&nbsp;Hassan D Kanaan,&nbsp;Ping L Zhang","doi":"10.1080/01913123.2023.2189341","DOIUrl":"https://doi.org/10.1080/01913123.2023.2189341","url":null,"abstract":"<p><strong>Background: </strong>Thrombotic microangiopathy (TMA) results in acute kidney injury, but the cause of heavy proteinuria in this disorder is puzzling. The goal of this study was to determine if there were significant effacement of foot processes and CD133-positive hyperplastic podocytes in TMA to explain the proteinuria.</p><p><strong>Methods: </strong>The study included 12 negative controls (renal parenchyma removed from renal cell carcinoma) and 28 thrombotic microangiopathy due to different etiologies. The percent of foot process effacement was estimated, and proteinuria level was obtained for each TMA case. Both groups of cases were stained for CD133 by immunohistochemical method, and the number of positive CD133 in hyperplastic podocytes was counted and analyzed.</p><p><strong>Results: </strong>Nineteen (19) of 28 (68%) TMA cases had nephrotic range proteinuria (urine protein/creatinine >3). Twenty-one (21) of 28 (75%) TMA cases showed positive CD133 staining in scattered hyperplastic podocytes within Bowman's space but was absent in control cases. The percent of foot process effacement (56 ± 4%) correlated with proteinuria (protein/creatinine ratio 4.4 ± 0.6) (<i>r</i> = 0.46, <i>p</i> = .0237) in TMA group.</p><p><strong>Conclusion: </strong>Our data indicate that the proteinuria in TMA can be associated with significant effacement of foot processes. CD133-positive hyperplastic podocytes can be seen in the majority of TMA cases of this cohort, indicating a partial podocytopathy.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"219-226"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9766800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse pulmonary effects after oral exposure to copper, manganese and mercury, alone and in mixtures, in a Spraque-Dawley rat model.","authors":"M Draper,&nbsp;Mj Bester,&nbsp;M Van Rooy,&nbsp;Hm Oberholzer","doi":"10.1080/01913123.2023.2184891","DOIUrl":"https://doi.org/10.1080/01913123.2023.2184891","url":null,"abstract":"<p><p>The rise in respiratory disease has been attributed to an increase in environmental pollution. Heavy metals contribute to environmental contamination via air, water, soil and food. The effects of atmospheric exposure to heavy metals on pulmonary structure and function have been researched, but the effects through drinking water have been neglected. The aim of this study was to investigate the potential <i>in vivo</i> alterations in the pulmonary tissue of male Sprague-Dawley rats after a 28-day oral exposure to copper (Cu), manganese (Mn) and mercury (Hg), alone and in mixtures, at 100 times the World Health Organization's (WHO) safety limit for each heavy metal in drinking water. Forty-eight male Sprague-Dawley rats were randomly divided into eight groups (n = 6): control, Cu, Mn, Hg, Cu + Mn, Cu + Hg, Mn + Hg and Cu, Mn + Hg. The morphology of lung tissue and the bronchioles were evaluated using light- and transmission electron microscopy. For all exposed groups, morphological changes included thickened inter- and intra-alveolar spaces, stratified epithelium, disrupted smooth muscle and early fibrosis and desquamation of the epithelia of the bronchioles to varying degrees. In all exposed groups, ultrastructurally, an increase in disarranged collagen and elastin fibers, nuclear membrane detachment, chromatin condensation, indistinct nucleoli and an increase in collagen fiber disarrangement was observed. This study has identified that oral exposure to Cu, Mn and Hg and as part of mixtures caused pathogenesis due to inflammation, cellular damage and fibrosis with Mn + Hg being the most potent heavy metal group.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"146-159"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9396709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome: a case report.","authors":"Yong-Xin Ru,&nbsp;Li Ying,&nbsp;Shu-Xu Dong,&nbsp;Hui-Ming Yi,&nbsp;Liu Jing,&nbsp;Zhang Yongqiang","doi":"10.1080/01913123.2023.2184892","DOIUrl":"https://doi.org/10.1080/01913123.2023.2184892","url":null,"abstract":"<p><p>A biopsy of gastrocnemius muscle from a patient with mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS) syndrome was studied histologically in semithin sections stained by hematoxylin-and-eosin (H&E) and toluidine blue, and ultrathin sections by transmission electron microscopy (TEM). H&E stain demonstrated typical ragged-red fibers (RRFs) and affected fibers in fascicles. Toluidine-blue stain showed an irregular meshwork in the center of RRFs. TEM demonstrated damaged myofibrils and variations in mitochondrial structure in RRFs and affected fibers. Dense mitochondria were compacted with cristae and pleomorphic electron-dense inclusions. Lucent mitochondria included paracrystalline inclusions with a parking lot appearance. At high magnification, the paracrystalline inclusions were composed of plates that paralleled and connected with mitochondrial cristae. These observations indicated that electron-dense granular and paracrystalline inclusions resulted from cristal degeneration and overlapping in mitochondria in MELAS syndrome.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"227-235"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9398134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study between the possible protective role of melatonin versus its combination with adipose derived-mesenchymal stem cells on experimentally induced diabetic retinopathy in adult male albino rats (Histological and immunohistochemical study).","authors":"Samar Reda,&nbsp;Ghada A Elsammak,&nbsp;Tamer G Elsayed,&nbsp;Samar Abdelaziz Mostafa","doi":"10.1080/01913123.2023.2184890","DOIUrl":"https://doi.org/10.1080/01913123.2023.2184890","url":null,"abstract":"<p><p>Diabetes mellitus is a metabolic disorder that can cause numerous ocular issues as well as long-term effects. In our study, we evaluate the effect of melatonin on the diabetic retinal alterations in male albino rats to the effect of melatonin combined with stem cells. 50 adult male rats were equally divided into four groups control, diabetic, melatonin, and melatonin plus stem cells. STZ, 65 mg/kg in phosphate buffered was administered intraperitoneally as a bolus to diabetic group of rats. After inducing diabetes, melatonin (10 mg/kg b.wt./day) was administered orally to the melatonin group for 8 weeks. The stem cell and melatonin group got the same dosage of melatonin as the prior group. They received an intravenous injection of (3?×?106 cell) adipose-derived MSC suspended in phosphate-buffered saline at same time of melatonin ingestion. Animals from all groups had their fundics examined. Following the injection of stem cells, samples of rat retina were collected for light and electron microscopy analyses. H&E and immunohistochemically stained sections revealed a slight improvement in group (III). At the same time, group (IV) results were comparable to those of the control group, which was supported by the findings of an electron microscope. Neovascularization was visible on fundus examination in group (II), whereas it was less noticeable in group (III) and group IV. Melatonin mildly improved the histological structure of the retina in diabetic rats, and when it was combined with adipose-derived MSC, it considerably improved the diabetic alterations.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 3","pages":"131-145"},"PeriodicalIF":1.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9454227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of hazards of electronic -cigarette's liquid refill on testes of mice, complemented by histopathological and chromatographic analysis.","authors":"Raghda Elsherif, Nora Z Abdellah, Ola A Hussein, Eman S Shaltout","doi":"10.1080/01913123.2023.2178569","DOIUrl":"10.1080/01913123.2023.2178569","url":null,"abstract":"<p><p>Electronic-cigarettes (e-cigarettes) are devices designed to become an alternative to classic cigarettes. Vaping of e-cigarettes and their recharge liquid have become extremely popular among the adolescents; however, its safety is not well established. Evaluation of the components of e-cigarette liquid and their potential effects on testis of adult male mice. This aim will be fulfilled by histological, ultrastructural, and immunohistochemical analysis of mice testis biopsies. Twenty mice were allocated into two groups of equal size. The control group was given regular saline, whereas the treated group was given e-liquid (contains 3 mg of nicotine/kg of body weight) both groups daily intraperitoneally injected for 3 weeks. Analysis of e-liquid by Gas Chromatography-Mass Spectrometric GC/MS demonstrated nicotine, phenol, vanillin, aldehydes, and pyrethroid insecticide. Evaluation of oxidative stress parameters revealed significant reduction of SOD and GPx. Histological results revealed a significant reduction in the height of seminiferous tubules, sloughing of spermatogenic cells, most cells being dark and pyknotic, and thickening of the interstitium with accumulation of PAS positive exudate. Most spermatogenic cells showed degenerative changes as rarefied cytoplasm, ill-defined electron-dense nuclei, and elongated spermatid showed deformity of ectoplasmic specialization. Immunohistochemical studies revealed a significant increase in caspase-3 positive cells and a significant reduction of area % of E-cadherin. The analysis of an available E-liquid demonstrated potentially harmful chemicals that are not shown in the labeling of the product. E-liquid appears to impair anti-oxidant defense and cause degenerative changes in the body and disruption of blood testes barrier BTB. So, e-cigarettes cannot be regarded as a non-harmful smoking replacement.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-14"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9336789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The possible protective role of vitamin C versus melatonin on potassium dichromate induced changes in rat thyroid gland: light and electron microscopic study.","authors":"Eman Saeed, Ahmed A El-Mansy, Shireen A Mazroa, Amal M Moustafa","doi":"10.1080/01913123.2023.2178568","DOIUrl":"10.1080/01913123.2023.2178568","url":null,"abstract":"<p><p>Potassium dichromate is widely used in various laboratory and industrial applications. Vitamin C and melatonin are well-known antioxidants. Study the microscopic and morphometric alterations in the thyroid gland in adult male albino rats after the administration of potassium dichromate for successive 2 months and also to assess the possible protective effect of vitamin C versus melatonin on these changes. Sixty adult male albino rats were randomly divided into four main groups. Group I (The control group). Group II received potassium dichromate (25 mg/kg/day) dissolved in distilled water by intraperitoneal (i.p) injection for 2 months. Group III received the same dose of potassium dichromate with vitamin C (120 mg/kg/day) orally through an intragastric intubation. Group IV received the same dose of potassium dichromate and melatonin (10 mg/kg/day) as an i.p injection. Thyroid gland samples were prepared for light and electron microscopic studies. Potassium dichromate group demonstrated congested blood vessels, follicular hyperplasia, follicular enlargement with degenerated lining cells that were exfoliated in the lumen. The parafollicular cells appeared with darkly stained nuclei. PAS reaction showed weak reaction in the colloid with an abnormal pattern of vacuolization. A highly significant increase in the percentage area of fibrosis was detected in Mallory trichrome sections. Ultrastructurally, follicular cells and parafollicular cells appeared irregular in shape with dark, small heterochromatic nuclei. Small, electron-dense granules in the parafollicular cells were found. Potassium dichromate and vitamin C-treated group III showed partial improvement of the thyroid gland. The PAS reaction showed that nearly all the follicles were more or less similar to those of the control group. A significant decrease in the percentage area of fibrosis in group III was found as compared to those in group II. Potassium dichromate and melatonin-treated group showed that the thyroid gland was nearly similar to that of the control group. Vitamin C and melatonin could partially protect against potassium dichromate induced changes in the thyroid gland and the protective effect of melatonin was better than that of vitamin C.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-17"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9336790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative survey of mitochondrial ultrastructure in <i>IDH1</i>-mutant astrocytoma and <i>IDH1</i>-wildtype glioblastoma (GBM).","authors":"Haitham H Maraqah, Mones S Abu-Asab, Han Sung Lee, Orwa Aboud","doi":"10.1080/01913123.2023.2175942","DOIUrl":"10.1080/01913123.2023.2175942","url":null,"abstract":"<p><p>Gliomas are the most common malignant brain tumors with poor prognosis. The WHO's classification recognizes isocitrate dehydrogenase 1 (<i>IDH1</i>) mutant astrocytoma and <i>IDH1</i>-wildtype glioblastoma (GBM). The <i>IDH1</i> mutation confers a survival advantage over the wildtype. There are several explanations for the metabolic advantage of the <i>IDH1</i> mutation, some involve mitochondrial implications. Since an ultrastructural comparison of both tumor genotypes is still lacking, we surveyed the ultrastructural effects of the <i>IDH1</i> mutation on the mitochondria of the <i>IDH1</i>-mutant astrocytoma (n = 15) and <i>IDH1</i>-wildtype glioblastoma (n = 15) tumors. Our results show that both <i>IDH1</i> genotypes have degenerate and uncoupled mitochondria; this has not been reported before. The presence of ample lipid inclusions and lipid droplets in the cytoplasm of both genotypes support our conclusion of dysfunctional uncoupled mitochondria. Thus, the <i>IDH1</i> mutation may have no ultrastructural consequences on the mitochondria, and the aberrant mitochondria in both genotypes may be the result of other unknown mutations. The status of the mitochondria in these genotypes portends a clinical challenge since tumor cells with uncoupled mitochondria are more primitive, aggressive, and considerably treatment resistant.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-6"},"PeriodicalIF":1.1,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10132693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}