Ultrastructural Pathology最新文献_第10页

Effect of ozone versus naringin on testicular injury in experimentally induced ulcerative colitis in adult male albino rats. 臭氧与柚皮苷对实验性溃疡性结肠炎成年雄性白化大鼠睾丸损伤的影响。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-09-03 DOI: 10.1080/01913123.2022.2132337

Abeer M Azmy, Bassant T Abd Elbaki, Mohammed A Ali, Abeer A Mahmoud

{"title":"Effect of ozone versus naringin on testicular injury in experimentally induced ulcerative colitis in adult male albino rats.","authors":"Abeer M Azmy, Bassant T Abd Elbaki, Mohammed A Ali, Abeer A Mahmoud","doi":"10.1080/01913123.2022.2132337","DOIUrl":"https://doi.org/10.1080/01913123.2022.2132337","url":null,"abstract":"Testicular dysfunction is caused by the continuous inflammation and oxidative stress that are present at the local site in ulcerative colitis (UC) spreading to the testes via systemic circulation. The influence of ozone and naringine on colitis-mediated testicular dysfunction was investigated in this study. Forty-eight adult male rats were divided into four groups: I control group, II dextran sodium sulfate (DSS) UC-induced group, III DSS+naringine, and IV DSS+ozone groups. UC was induced in groups II, III, and IV using 0.1 ml of 4% DSS in their drinking water per day for 6 days by gastric gavage. All animals were sacrificed 45 days from the start. Blood samples were obtained to estimate serum testosterone hormone. Testicular tissues were processed for measurement of tissue malondialdehyde (MDA) and examined by light and electron microscopes. Ultrastructurally, group II revealed a relatively thick basement membrane enveloping the seminiferous tubule. Sertoli cell cytoplasm appears rarified with wide intracellular spaces, vacuoles, and multiple lysosomes; distorted spermatogonia with electron dense nuclei and cytoplasm; and primary spermatocytes with small nuclei and electron dense cytoplasm. Abnormal sperm profiles were visible in middle pieces, mid, principle, and end pieces that were markedly affected with disorganization of axoneme and outer dense fibers. Leydig cells revealed dilated cisternae of smooth endoplasmic reticulum. Morphometric and statistical analyses were performed. Group III showed some improvement; however, group IV showed more improvement. The results indicated that ozone caused marked improvement than naringine against UC-induced testicular damage via their antioxidant and anti-inflammatory properties.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33501615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The ultrastructure of muscle fibers and satellite cells in experimental autoimmune encephalomyelitis after treatment with transcranial magnetic stimulation. 经颅磁刺激治疗实验性自身免疫性脑脊髓炎后肌纤维和卫星细胞的超微结构。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-09-03 Epub Date: 2022-08-22 DOI: 10.1080/01913123.2022.2112330

María Angeles Peña-Toledo, Evelio Luque, Manuel LaTorre, Ignacio Jimena, Fernando Leiva-Cepas, Ignacio Ruz-Caracuel, Eduardo Agüera, J Peña-Amaro, Isaac Tunez

{"title":"The ultrastructure of muscle fibers and satellite cells in experimental autoimmune encephalomyelitis after treatment with transcranial magnetic stimulation.","authors":"María Angeles Peña-Toledo, Evelio Luque, Manuel LaTorre, Ignacio Jimena, Fernando Leiva-Cepas, Ignacio Ruz-Caracuel, Eduardo Agüera, J Peña-Amaro, Isaac Tunez","doi":"10.1080/01913123.2022.2112330","DOIUrl":"https://doi.org/10.1080/01913123.2022.2112330","url":null,"abstract":"In this study, we investigated the effect of transcranial magnetic stimulation (TMS) on the ultrastructure of muscle fibers and satellite cells in rats with experimental autoimmune encephalomyelitis (EAE). EAE-induced animals were treated with TMS (60 Hz at 0.7 mT) for 2 hours in the morning, once a day, 5 days a week, for 3 weeks, starting on day 15 post-immunization. The rats were sacrificed on day 36 post-immunization, and the soleus muscles were evaluated by light microscopy and transmission electron microscopy. Findings were compared with a non-treated EAE group. Electron microscopy analysis showed the presence of degenerated mitochondria, autophagic vacuoles, and altered myofibrils in non-treated EAE group. This correlates with the presence of acid phosphatase activity in muscle fibers and core-targetoid lesions with desmin immunohistochemistry. Most myonuclei in the EAE group showed apoptotic features. In contrast, EAE induced-TMS treated animals had less ultrastructural changes in the mitochondria and the myofibrils, together with less frequent apoptotic nuclear features. Peripheral desmin+ protrusions, as a marker of active satellite cells, were significantly increased in TMS-treated group. This correlates ultrastructurally with the presence of active features in satellite cells in the TMS group. In conclusion, the attenuation of ultrastructural alterations in muscle fibers and activation response of satellite cells caused by EAE indicated that skeletal muscle had a regenerative response to TMS.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40434299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and ultrastructural changes in the skeletal muscles of dysferlin-deficient mice during postnatal ontogenesis. 出生后个体发育过程中异常蛋白缺乏小鼠骨骼肌的结构和超微结构变化。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-07-04 Epub Date: 2022-07-26 DOI: 10.1080/01913123.2022.2105464

O N Chernova, I A Chekmareva, M O Mavlikeev, I A Yakovlev, A P Kiyasov, R V Deev

{"title":"Structural and ultrastructural changes in the skeletal muscles of dysferlin-deficient mice during postnatal ontogenesis.","authors":"O N Chernova, I A Chekmareva, M O Mavlikeev, I A Yakovlev, A P Kiyasov, R V Deev","doi":"10.1080/01913123.2022.2105464","DOIUrl":"https://doi.org/10.1080/01913123.2022.2105464","url":null,"abstract":"A number of sarcolemma proteins are responsible for muscle fiber repair. Dysferlin encoded by the DYSF gene is one of these proteins. Dysferlin promotes membrane repair in striated muscle fibers (MFs). Mutations in DYSF lead to loss of or decreased dysferlin expression, impaired membrane repair in MF, and its destruction, clinically manifesting as dysferlinopathy. Preclinical studies of cell and gene therapies aimed at restoring impaired muscle regeneration require well-characterized small animal models. Our investigation aimed to distinguish the histopathological features of a mouse strain lacking dysferlin expression (Bla/J strain). Ultrastructural changes in the sarcolemma, mitochondria and contractile apparatus were observed. It was shown that postnatal histogenesis of skeletal muscles in genetically determined dysferlin deficiency is characterized by a higher proportion of necrotic muscle fibers, compensatory hypertrophy of muscle fibers with their subsequent atrophy, and decreases in proliferative activity and the level of myogenic differentiation of myogenic progenitor cells compared to wild-type mice (C57Bl/6).","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40635516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anticancer activity of linalool: comparative investigation of ultrastructural changes and apoptosis in breast cancer cells. 芳樟醇抗肿瘤活性:对乳腺癌细胞超微结构变化及凋亡的比较研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-07-04 Epub Date: 2022-06-21 DOI: 10.1080/01913123.2022.2091068

Hulya Elbe, Feral Ozturk, Gurkan Yigitturk, Tuba Baygar, Turker Cavusoglu

{"title":"Anticancer activity of linalool: comparative investigation of ultrastructural changes and apoptosis in breast cancer cells.","authors":"Hulya Elbe, Feral Ozturk, Gurkan Yigitturk, Tuba Baygar, Turker Cavusoglu","doi":"10.1080/01913123.2022.2091068","DOIUrl":"https://doi.org/10.1080/01913123.2022.2091068","url":null,"abstract":"Breast cancer is the most common cancer in women ın the world. Many anticancer drugs are currently used clinically have been isolated from plant species or are based on such substances. Linalool is aromatic compounds from the monoterpene group. It is the main constituents of essential oils and show antiproliferative, antioxidant, and antiseptic properties. The aim of this study was to investigate the antiproliferativeand apoptotic, effects of linalool in MCF-7 and MDA-MB-231 human breast cancer cells. MCF-7 and MDA-MB-231 human breast cancer cells were treated with different concentrations of linalool (100, 200, 400, 600, 800, 1000 µM) at 24 h and 48 h. MTT assay for cell proliferation and Annexin V assay for apoptosis was done. The morphology of breast cancer cells was investigated by lıght mıcroscope and scanning electron microscope (SEM). The study show that linalool significantly induced apoptosis in all groups as dose and time-dependent (p < .05). Linalool has apoptotic and antiproliferative properties in a concentration and time-dependent manner in breast cancer cells. The cytotoxic effects of linalool on MCF-7 and MDA-MB-231 human breast cancer cells was found to be associated with apoptotic cell death. Linalool was more effective on MCF-7 human breast cancer cells in smaller amounts.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40141041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Congenital granular cell epulis: 24 new cases with more differences than similarities to granular cell tumor. 先天性颗粒细胞性脓肿:24例新发与颗粒细胞瘤异同多。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-07-04 DOI: 10.1080/01913123.2022.2107750

Hernandez Sara Avalos, Elizabeth Manci, Madhuri Mulekar, Aisling Finnegan, Sandip Barui, Carlos Galliani, David Kelly, Guillermo A Herrera

{"title":"Congenital granular cell epulis: 24 new cases with more differences than similarities to granular cell tumor.","authors":"Hernandez Sara Avalos, Elizabeth Manci, Madhuri Mulekar, Aisling Finnegan, Sandip Barui, Carlos Galliani, David Kelly, Guillermo A Herrera","doi":"10.1080/01913123.2022.2107750","DOIUrl":"https://doi.org/10.1080/01913123.2022.2107750","url":null,"abstract":"Congenital granular cell epulis (CGCE) is a rare tumor of gingiva that is exclusive to newborns, has marked female predominance, and is rarely associated with other abnormalities. Although benign in behavior, CGCE can be lethal by obstruction of respiration and/or deglutition and can require a multidisciplinary team of specialist at birth for survival of an otherwise normal infant. Histologically, CGCE resembles granular cell tumor (GCT), but unlike GCT, which is Schwannian-derived, derivation of CGCE remains an enigma, largely because of its low prevalence. This study presents 24 new cases of CGCE, the largest series since the original description 150 years ago and permits detailed study of homogeneity of cases diagnosed as CGCE as well as detailed comparisons of CGCE with GCT by clinical, morphological, immunohistochemical, and ultrastructural studies. The data show homogeneity within the CGCE cases, more differences than similarities between CGCE and GCT, and no immunohistochemical staining for common placental proteins/hormones in CGCE. The findings support a primitive mesenchymal cell origin, and a progressive degenerative process in CGCE, rather than neoplasia. Prenatal detection of this lesion is important to facilitate adequate preparations for support of these infants during labor and delivery.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33493631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of pulsed radiofrequency application on nerve healing after sciatic nerve anastomosis in rats. 脉冲射频应用对大鼠坐骨神经吻合术后神经愈合的影响。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-07-04 Epub Date: 2022-07-22 DOI: 10.1080/01913123.2022.2066237

Uğur Ö Bayır, Recep Aksu, Özlem Öz Gergin, Gozde Ozge Onder, Leman Sencar, Eray Günay, Arzu H Yay, İbrahim Karaman, Cihangir Bicer, Sait Polat

{"title":"The effect of pulsed radiofrequency application on nerve healing after sciatic nerve anastomosis in rats.","authors":"Uğur Ö Bayır, Recep Aksu, Özlem Öz Gergin, Gozde Ozge Onder, Leman Sencar, Eray Günay, Arzu H Yay, İbrahim Karaman, Cihangir Bicer, Sait Polat","doi":"10.1080/01913123.2022.2066237","DOIUrl":"https://doi.org/10.1080/01913123.2022.2066237","url":null,"abstract":"The Effect of Pulsed Radiofrequency Application on Nerve Healing After Sciatic Nerve Anastomosis in Rats. In this study, we aimed to evaluate the histomorphological and functional effect of Pulsed Radiofrequency (PRF) application on regeneration after experimental nerve damage in rats. Forty Sprague-Dawley male rats were used in the study. Sciatic nerve incision was applied to all rats and then anastomosis was performed. Twenty rats were separated as the control group, and the remaining 20 rats underwent PRF every day at 42oC, for 120 seconds. The groups were divided into two further subgroups to be sacrificed on the 15th and 30th days. Tissue samples were obtained from all groups at 24 hours and 72 hours after the injury. Sections of sciatic nerve samples were stained with hematoxylin-eosin for light microscopic investigation and prepared for evaluation of ultrastructural changes with transmission electron microscopy. In the evaluation of axon numbers and diameters were seen that the 30th-day RF group had an increase compared to the control group. In the electron microscopic examination, it was observed that myelinated and unmyelinated nerve fiber sheaths had borders that are more regular in the RF group, the nucleus structures of schwann cells were better preserved, mitochondrial damage was less, and the extensions of fibroblast and collagen fibers were smoother than the control group. The findings suggested that PRF application has a positive contribution histologically on nerve healing in the early period after full-layer incision nerve injury anastomosis surgery.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40614912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of erythromycin on the ultrastructure of human macrophages exposed to cigarette smoke extract in vitro. 红霉素对体外暴露于香烟烟雾提取物的人巨噬细胞超微结构的影响

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-07-04 Epub Date: 2022-06-10 DOI: 10.1080/01913123.2022.2060395

Shaoshuang Wang, Jianjun Huo, Yanlin Wei, Mei Huan, Zhouling Luo, Meihua Li, Mingzhi Wen, Xiaoning Zhong, Zhiyi He, Nan Ma, Jufeng Qiu, Xiaojuan Tang

{"title":"Effect of erythromycin on the ultrastructure of human macrophages exposed to cigarette smoke extract in vitro.","authors":"Shaoshuang Wang, Jianjun Huo, Yanlin Wei, Mei Huan, Zhouling Luo, Meihua Li, Mingzhi Wen, Xiaoning Zhong, Zhiyi He, Nan Ma, Jufeng Qiu, Xiaojuan Tang","doi":"10.1080/01913123.2022.2060395","DOIUrl":"10.1080/01913123.2022.2060395","url":null,"abstract":"Macrophages serve an active role in the pathophysiology of chronic obstructive pulmonary disease (COPD). Erythromycin (EM) has been verified as an effective treatment for COPD. However, there are few studies on the effect of EM on the ultrastructure of macrophages exposed to cigarette smoke extract (CSE). In the present study, human macrophages were randomly divided into three groups: The control, CSE and the CSE+EM group, using electron microscopy, the effect of EM was evaluated by comparing the ultrastructural changes between these groups. The macrophages were additionally divided into a further four groups: The control, CSE, CSE+EM 24 h and CSE+EM 48 h groups. The generation of reactive oxygen species (ROS) in each group was evaluated by detecting fluorescence intensity. It was observed that the cellular ultrastructure of the CSE group exhibited abnormal changes, though this effect was reversed back to the level of the control in the CSE+EM group. Compared with the control group, the ROS expression level was significantly increased in the CSE group (P < .05); however, compared with the CSE group, the ROS concentration was decreased in the CSE+EM 24 h (P < .05) and CSE+EM 48 h groups (P < .05), though this was more apparent in the EM 48 h group. It was concluded that EM protects human macrophages against CSE. Moreover, it was hypothesized that EM may reduce the symptoms of patients with COPD by protecting the macrophage ultrastructure from the effects of CSE, resulting in the decreased generation of ROS, inhibiting autophagy and reducing endoplasmic reticulum stress.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43950003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subepithelial deposits with microspherular structures in membranous glomerulonephritis 膜性肾小球肾炎中具有微球结构的上皮下沉积

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-06-16 DOI: 10.1080/01913123.2022.2090646

H. Choung, J. Jean-Gilles, B. Goldman

{"title":"Subepithelial deposits with microspherular structures in membranous glomerulonephritis","authors":"H. Choung, J. Jean-Gilles, B. Goldman","doi":"10.1080/01913123.2022.2090646","DOIUrl":"https://doi.org/10.1080/01913123.2022.2090646","url":null,"abstract":"ABSTRACT Rare cases of membranous glomerulopathy (MGN) with subepithelial deposits consisting of microspherular structures identified by electron microscopy have been described in the literature as either MGN with spherules or podocyte infolding glomerulopathy (PIG). The paucity of available studies shows a strong association with underlying autoimmune disease. To further understand the significance of subepithelial microspherular deposits, we retrospectively identified native kidney biopsies from 10 patients diagnosed as MGN with subepithelial microspherular structures identified by ultrastructural examination at the University of Rochester Medical Center (URMC) during an 11-year period. The majority were Caucasian (80%) with a mean age of 51.3 (±12.9) years. 50% had an autoimmune disorder, of which 80% were SLE. Two SLE cases had concomitant rheumatoid arthritis and Sjogren’s syndrome. One additional case had antiphospholipid syndrome and showed lupus-like features on biopsy. 40% were idiopathic and negative for PLA2R, NELL1, and THSD7A. MGN with subepithelial microspherular structures is frequently associated with an underlying autoimmune disease. The majority are negative for markers of primary MGN (PLA2R, THSD7A, and NELL1) and show features suggestive of secondary MGN.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42138654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Electron microscopic study on the effect of chronic fluoxetine treatment on pituitary gland and the possible therapeutic effect of adipose-derived mesenchymal stem cells in adult male albino rats 慢性氟西汀对成年雄性白化大鼠垂体的影响及脂肪间充质干细胞可能的治疗作用的电镜研究

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-06-13 DOI: 10.1080/01913123.2022.2083279

Nashwa S. Wahba, Assmaa O Seliem, Enas G Abd Allah, Maha Z. Mohammed

{"title":"Electron microscopic study on the effect of chronic fluoxetine treatment on pituitary gland and the possible therapeutic effect of adipose-derived mesenchymal stem cells in adult male albino rats","authors":"Nashwa S. Wahba, Assmaa O Seliem, Enas G Abd Allah, Maha Z. Mohammed","doi":"10.1080/01913123.2022.2083279","DOIUrl":"https://doi.org/10.1080/01913123.2022.2083279","url":null,"abstract":"ABSTRACT Background: Adipose-derived mesenchymal stem cells (ADSCs) have therapeutic potential for the treatment of a variety of disorders due to their self-renewal and multipotential differentiation capabilities. Aim of the work: This study was planned to demonstrate the electron microscopic structure of the pituitary gland after chronic fluoxetine treatment and the possible therapeutic effect of ADSCs. Materials and methods:Thirty healthy male adult albino rats were classified into Control group (Group I). Fluoxetine treated (Group II) received 24 mg/kg/day of fluoxetine dissolved in 1.0 mL of tap water once a day. Fluoxetine group treated with ADSCs (Group III) received fluoxetine as group (II) for 30 days and then was injected once by ADSCs at a dose of 1 × 106 cells/rat in the tail vein suspended in 0.5 ml of phosphate-buffered saline (PBS). Recovery group (Group IV) received fluoxetine for 30 days and then received no treatment till the end of the experiment. Results: The ultrastructural observations of the fluoxetine-treated group revealed major histological changes in both the pars distalis and nervosa. Pars distalis revealed cells with different shapes, sizes, nuclei, and variable profiles of the cytoplasm. Pars nervosa, on the other hand, revealed pituicytes with electron-lucent cytoplasm and small apoptotic nuclei. Administration of ADSCs greatly improved the microscopic appearance of cells, while the recovery group showed similar histological changes as the fluoxetine group. Conclusion: Fluoxetine caused various deleterious changes in the pituitary gland of albino rats, as evidenced by electron microscopy. These changes were almost corrected by the ADSCs treatment. .","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45886887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of Vitamin D on testosterone and uncarboxylated osteocalcin levels in aged male rats 维生素D对老年雄性大鼠睾酮和未羧化骨钙素水平的影响

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-06-08 DOI: 10.1080/01913123.2022.2083280

G. Coşkun, L. Sencar, A. Tuli, Ebru Dundar Yenilmez, S. Polat

{"title":"The effect of Vitamin D on testosterone and uncarboxylated osteocalcin levels in aged male rats","authors":"G. Coşkun, L. Sencar, A. Tuli, Ebru Dundar Yenilmez, S. Polat","doi":"10.1080/01913123.2022.2083280","DOIUrl":"https://doi.org/10.1080/01913123.2022.2083280","url":null,"abstract":"ABSTRACT There is a well-established complex interaction between vitamin D metabolism and bone and gonad functions. In this study, we aimed to investigate the potential effects of vitamin D therapy on testosterone and osteocalcin (OC) levels in aged male rats. Forty-five adult male rats were divided into three groups in this study. Unlike the control group, the two experimental groups received 50 IU/kg/day and 100 IU/kg/day of vitamin D3 (cholecalciferol), respectively, for a 4-week period using the gavage method. Testicular tissue and blood samples from rats were collected under general anesthesia at the end of the 4-week period. Testicular tissue samples were examined using light and electron microscopy. Additionally, serum testosterone and OC levels were measured in blood samples. The 50 IU/kg dose of cholecalciferol increased testosterone and OC levels, which were lower than normal due to aging, and regulated the organization of the seminiferous tubule epithelium and interstitium more effectively than the 100 IU/kg dose of cholecalciferol. Male fertility functions and bone health, which degrade due to aging, were increased due to the use of exogenous vitamin D, although the higher dose was not associated with more effective results.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44866591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0