Ultrastructural Pathology最新文献_第10页

Methotrexate enhances oxidative stress, apoptosis, and ultrastructural alterations in the placenta of rat. 甲氨蝶呤增强大鼠胎盘氧化应激、细胞凋亡和超微结构改变。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-11-02 DOI: 10.1080/01913123.2022.2154877

Amany Mohamed Shalaby, Khalid Mohammed Mohammed Albakoush, Mohamed Ali Alabiad, Mohammed Alorini, Fatima A Jaber, Mahmoud Ramadan Elkholy, Shereen Elsayed Tawfeek

{"title":"Methotrexate enhances oxidative stress, apoptosis, and ultrastructural alterations in the placenta of rat.","authors":"Amany Mohamed Shalaby, Khalid Mohammed Mohammed Albakoush, Mohamed Ali Alabiad, Mohammed Alorini, Fatima A Jaber, Mahmoud Ramadan Elkholy, Shereen Elsayed Tawfeek","doi":"10.1080/01913123.2022.2154877","DOIUrl":"https://doi.org/10.1080/01913123.2022.2154877","url":null,"abstract":"The chemotherapeutic drug methotrexate (MTX) is utilized to treat various malignancies. MTX exposure during pregnancy causes miscarriages, abnormalities in newborns, and developmental delays. The current study examined the placenta's sequential histopathological alterations following exposure to the MTX in pregnant rats. Twenty-four pregnant rats were assigned into; the control group and MTX group (0.2 mg/kg). MTX was given intraperitoneally on gestational days 11-12. Oxidative stress parameters were measured in placental homogenates. The placental specimens were evaluated by light, immunohistochemical (caspase-3 and vascular endothelial growth factor (VEGF)), and electron microscopic study. Malondialdehyde levels were significantly elevated by MTX, whereas glutathione peroxidase and superoxide dismutase levels were significantly reduced. The MTX group showed a marked reduction in the thickness of both the basal and labyrinth zones. Degeneration of the labyrinth zone was demonstrated. Also, giant trophoblast cells and the spongiotrophoblasts of the basal zone showed vacuolations with dark nuclei. Up-regulation of caspase-3 and down-regulation of VEGF immunoexpression were demonstrated. Ultrastructurally, disintegration of the interhemal membrane, spongiotrophoblasts with vacuolated cytoplasm and small condensed nuclei, and the giant trophoblasts with irregular nuclear outlines and vacuolated cytoplasm were demonstrated. In conclusion, MTX has profoundly altered the structure of the placenta.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 6","pages":"531-541"},"PeriodicalIF":1.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10351785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural changes induced by açaí (Euterpe oleracea Mart) in MCF-7 breast cancer cell line. açaí (Euterpe oleracea Mart)诱导MCF-7乳腺癌细胞系超微结构改变。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-11-02 DOI: 10.1080/01913123.2022.2141404

Marcos Antonio Custódio Neto da Silva, Camila Simões Soares, Kátia Regina Assunção Borges, Laís Araujo Souza Wolff, Maria Do Carmo Lacerda Barbosa, Maria Do Desterro Soares Brandão Nascimento, João Ernesto de Carvalho

{"title":"Ultrastructural changes induced by açaí (Euterpe oleracea Mart) in MCF-7 breast cancer cell line.","authors":"Marcos Antonio Custódio Neto da Silva, Camila Simões Soares, Kátia Regina Assunção Borges, Laís Araujo Souza Wolff, Maria Do Carmo Lacerda Barbosa, Maria Do Desterro Soares Brandão Nascimento, João Ernesto de Carvalho","doi":"10.1080/01913123.2022.2141404","DOIUrl":"https://doi.org/10.1080/01913123.2022.2141404","url":null,"abstract":"Açaí (Euterpe oleracea Mart) is an Amazon plant with many biological properties. Previous report of this group evidenced autophagy induction after treatment with açaí seed extract in MCF-7 breast cancer cell lines by acridine orange assay. The aim of this study was to evaluate the ultrastructural changes induced by açaí seed extract in MCF-7 breast cancer cell lines. First, MCF- 7 breast cancer cell line viability was evaluated by MTT assay. Acridine orange assay showed increase in the acidic compartments, suggesting autophagolysosome formation. These cells were treated with 25 μg/ml for 24 h and evaluated by transmission electron microscopy (MET). This analysis showed that açaí seed extract induced autophagy, confirmed by autophagolysosome formation. Furthermore, açaí seed extract increased the number of mitochondria, suggesting the enrollment of reactive oxygen species in autophagy.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 6","pages":"511-518"},"PeriodicalIF":1.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10700740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The possible protective role of pimpinella anisum oil versus selenium on aspartame induced changes in rat cerebellar cortex: histological, immunohistochemical and electron microscopic study. 茴香油与硒对阿斯巴甜诱导的大鼠小脑皮层变化的保护作用:组织学、免疫组化和电镜研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-11-02 DOI: 10.1080/01913123.2022.2136809

Amira I Shrief, Ahmed A M Abdel-Hamid, Am Moustafa, E El-Mohandes

{"title":"The possible protective role of pimpinella anisum oil versus selenium on aspartame induced changes in rat cerebellar cortex: histological, immunohistochemical and electron microscopic study.","authors":"Amira I Shrief, Ahmed A M Abdel-Hamid, Am Moustafa, E El-Mohandes","doi":"10.1080/01913123.2022.2136809","DOIUrl":"https://doi.org/10.1080/01913123.2022.2136809","url":null,"abstract":"Aspartame (ASP) is an artificial sweeter. Chronic use of ASP has a harmful effect on cerebellar cortex. Anisum oil and selenium (SE) are antioxidant substances. Therefore, the present study was performed to study the possible protective role of anisum oil versus selenium on aspartame-induced changes in rat cerebellar cortex. Rats were divided into four main groups. Group I (Control group). Group II received 250 mg/kg/day aspartame once daily for 2 months. Group III received 0.5 ml/kg/day anisum 2 h before aspartame administration. Group IV received 0.5 mg/kg/day selenium 2 h before aspartame administration. The administration of Asp for 2 months (group II) resulted in cerebellar histopathological changes in the form of deformed Purkinje and granule cells. Ultrastructurally, Purkinje cells had irregular nuclei, dilated cisternae of rough endoplasmic reticulum, dilated saccules of Golgi apparatus, mitochondria with destroyed cristae. In addition, granule cells appeared shrunken with irregular nuclei. Aspartame and anisum oil treated group (group III) showed partial improvement. Examination of ASP and SE treated group (group IV) showed that cerebellar cortex was nearly similar to control. In conclusion, Anisum oil and selenium could protect against ASP-induced cerebellar damage. The protective effect of selenium is better than anisum oil.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 6","pages":"497-510"},"PeriodicalIF":1.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10342824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of ozone versus naringin on testicular injury in experimentally induced ulcerative colitis in adult male albino rats. 臭氧与柚皮苷对实验性溃疡性结肠炎成年雄性白化大鼠睾丸损伤的影响。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-09-03 DOI: 10.1080/01913123.2022.2132337

Abeer M Azmy, Bassant T Abd Elbaki, Mohammed A Ali, Abeer A Mahmoud

{"title":"Effect of ozone versus naringin on testicular injury in experimentally induced ulcerative colitis in adult male albino rats.","authors":"Abeer M Azmy, Bassant T Abd Elbaki, Mohammed A Ali, Abeer A Mahmoud","doi":"10.1080/01913123.2022.2132337","DOIUrl":"https://doi.org/10.1080/01913123.2022.2132337","url":null,"abstract":"Testicular dysfunction is caused by the continuous inflammation and oxidative stress that are present at the local site in ulcerative colitis (UC) spreading to the testes via systemic circulation. The influence of ozone and naringine on colitis-mediated testicular dysfunction was investigated in this study. Forty-eight adult male rats were divided into four groups: I control group, II dextran sodium sulfate (DSS) UC-induced group, III DSS+naringine, and IV DSS+ozone groups. UC was induced in groups II, III, and IV using 0.1 ml of 4% DSS in their drinking water per day for 6 days by gastric gavage. All animals were sacrificed 45 days from the start. Blood samples were obtained to estimate serum testosterone hormone. Testicular tissues were processed for measurement of tissue malondialdehyde (MDA) and examined by light and electron microscopes. Ultrastructurally, group II revealed a relatively thick basement membrane enveloping the seminiferous tubule. Sertoli cell cytoplasm appears rarified with wide intracellular spaces, vacuoles, and multiple lysosomes; distorted spermatogonia with electron dense nuclei and cytoplasm; and primary spermatocytes with small nuclei and electron dense cytoplasm. Abnormal sperm profiles were visible in middle pieces, mid, principle, and end pieces that were markedly affected with disorganization of axoneme and outer dense fibers. Leydig cells revealed dilated cisternae of smooth endoplasmic reticulum. Morphometric and statistical analyses were performed. Group III showed some improvement; however, group IV showed more improvement. The results indicated that ozone caused marked improvement than naringine against UC-induced testicular damage via their antioxidant and anti-inflammatory properties.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 5","pages":"439-461"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33501615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Congenital granular cell epulis: 24 new cases with more differences than similarities to granular cell tumor. 先天性颗粒细胞性脓肿:24例新发与颗粒细胞瘤异同多。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-07-04 DOI: 10.1080/01913123.2022.2107750

Hernandez Sara Avalos, Elizabeth Manci, Madhuri Mulekar, Aisling Finnegan, Sandip Barui, Carlos Galliani, David Kelly, Guillermo A Herrera

{"title":"Congenital granular cell epulis: 24 new cases with more differences than similarities to granular cell tumor.","authors":"Hernandez Sara Avalos, Elizabeth Manci, Madhuri Mulekar, Aisling Finnegan, Sandip Barui, Carlos Galliani, David Kelly, Guillermo A Herrera","doi":"10.1080/01913123.2022.2107750","DOIUrl":"https://doi.org/10.1080/01913123.2022.2107750","url":null,"abstract":"Congenital granular cell epulis (CGCE) is a rare tumor of gingiva that is exclusive to newborns, has marked female predominance, and is rarely associated with other abnormalities. Although benign in behavior, CGCE can be lethal by obstruction of respiration and/or deglutition and can require a multidisciplinary team of specialist at birth for survival of an otherwise normal infant. Histologically, CGCE resembles granular cell tumor (GCT), but unlike GCT, which is Schwannian-derived, derivation of CGCE remains an enigma, largely because of its low prevalence. This study presents 24 new cases of CGCE, the largest series since the original description 150 years ago and permits detailed study of homogeneity of cases diagnosed as CGCE as well as detailed comparisons of CGCE with GCT by clinical, morphological, immunohistochemical, and ultrastructural studies. The data show homogeneity within the CGCE cases, more differences than similarities between CGCE and GCT, and no immunohistochemical staining for common placental proteins/hormones in CGCE. The findings support a primitive mesenchymal cell origin, and a progressive degenerative process in CGCE, rather than neoplasia. Prenatal detection of this lesion is important to facilitate adequate preparations for support of these infants during labor and delivery.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 4","pages":"388-400"},"PeriodicalIF":1.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33493631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of erythromycin on the ultrastructure of human macrophages exposed to cigarette smoke extract in vitro. 红霉素对体外暴露于香烟烟雾提取物的人巨噬细胞超微结构的影响

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-07-04 Epub Date: 2022-06-10 DOI: 10.1080/01913123.2022.2060395

Shaoshuang Wang, Jianjun Huo, Yanlin Wei, Mei Huan, Zhouling Luo, Meihua Li, Mingzhi Wen, Xiaoning Zhong, Zhiyi He, Nan Ma, Jufeng Qiu, Xiaojuan Tang

{"title":"Effect of erythromycin on the ultrastructure of human macrophages exposed to cigarette smoke extract in vitro.","authors":"Shaoshuang Wang, Jianjun Huo, Yanlin Wei, Mei Huan, Zhouling Luo, Meihua Li, Mingzhi Wen, Xiaoning Zhong, Zhiyi He, Nan Ma, Jufeng Qiu, Xiaojuan Tang","doi":"10.1080/01913123.2022.2060395","DOIUrl":"10.1080/01913123.2022.2060395","url":null,"abstract":"Macrophages serve an active role in the pathophysiology of chronic obstructive pulmonary disease (COPD). Erythromycin (EM) has been verified as an effective treatment for COPD. However, there are few studies on the effect of EM on the ultrastructure of macrophages exposed to cigarette smoke extract (CSE). In the present study, human macrophages were randomly divided into three groups: The control, CSE and the CSE+EM group, using electron microscopy, the effect of EM was evaluated by comparing the ultrastructural changes between these groups. The macrophages were additionally divided into a further four groups: The control, CSE, CSE+EM 24 h and CSE+EM 48 h groups. The generation of reactive oxygen species (ROS) in each group was evaluated by detecting fluorescence intensity. It was observed that the cellular ultrastructure of the CSE group exhibited abnormal changes, though this effect was reversed back to the level of the control in the CSE+EM group. Compared with the control group, the ROS expression level was significantly increased in the CSE group (P < .05); however, compared with the CSE group, the ROS concentration was decreased in the CSE+EM 24 h (P < .05) and CSE+EM 48 h groups (P < .05), though this was more apparent in the EM 48 h group. It was concluded that EM protects human macrophages against CSE. Moreover, it was hypothesized that EM may reduce the symptoms of patients with COPD by protecting the macrophage ultrastructure from the effects of CSE, resulting in the decreased generation of ROS, inhibiting autophagy and reducing endoplasmic reticulum stress.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 1","pages":"303-312"},"PeriodicalIF":1.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43950003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subepithelial deposits with microspherular structures in membranous glomerulonephritis 膜性肾小球肾炎中具有微球结构的上皮下沉积

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-06-16 DOI: 10.1080/01913123.2022.2090646

H. Choung, J. Jean-Gilles, B. Goldman

{"title":"Subepithelial deposits with microspherular structures in membranous glomerulonephritis","authors":"H. Choung, J. Jean-Gilles, B. Goldman","doi":"10.1080/01913123.2022.2090646","DOIUrl":"https://doi.org/10.1080/01913123.2022.2090646","url":null,"abstract":"ABSTRACT Rare cases of membranous glomerulopathy (MGN) with subepithelial deposits consisting of microspherular structures identified by electron microscopy have been described in the literature as either MGN with spherules or podocyte infolding glomerulopathy (PIG). The paucity of available studies shows a strong association with underlying autoimmune disease. To further understand the significance of subepithelial microspherular deposits, we retrospectively identified native kidney biopsies from 10 patients diagnosed as MGN with subepithelial microspherular structures identified by ultrastructural examination at the University of Rochester Medical Center (URMC) during an 11-year period. The majority were Caucasian (80%) with a mean age of 51.3 (±12.9) years. 50% had an autoimmune disorder, of which 80% were SLE. Two SLE cases had concomitant rheumatoid arthritis and Sjogren’s syndrome. One additional case had antiphospholipid syndrome and showed lupus-like features on biopsy. 40% were idiopathic and negative for PLA2R, NELL1, and THSD7A. MGN with subepithelial microspherular structures is frequently associated with an underlying autoimmune disease. The majority are negative for markers of primary MGN (PLA2R, THSD7A, and NELL1) and show features suggestive of secondary MGN.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 1","pages":"377 - 387"},"PeriodicalIF":1.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42138654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Electron microscopic study on the effect of chronic fluoxetine treatment on pituitary gland and the possible therapeutic effect of adipose-derived mesenchymal stem cells in adult male albino rats 慢性氟西汀对成年雄性白化大鼠垂体的影响及脂肪间充质干细胞可能的治疗作用的电镜研究

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-06-13 DOI: 10.1080/01913123.2022.2083279

Nashwa S. Wahba, Assmaa O Seliem, Enas G Abd Allah, Maha Z. Mohammed

{"title":"Electron microscopic study on the effect of chronic fluoxetine treatment on pituitary gland and the possible therapeutic effect of adipose-derived mesenchymal stem cells in adult male albino rats","authors":"Nashwa S. Wahba, Assmaa O Seliem, Enas G Abd Allah, Maha Z. Mohammed","doi":"10.1080/01913123.2022.2083279","DOIUrl":"https://doi.org/10.1080/01913123.2022.2083279","url":null,"abstract":"ABSTRACT Background: Adipose-derived mesenchymal stem cells (ADSCs) have therapeutic potential for the treatment of a variety of disorders due to their self-renewal and multipotential differentiation capabilities. Aim of the work: This study was planned to demonstrate the electron microscopic structure of the pituitary gland after chronic fluoxetine treatment and the possible therapeutic effect of ADSCs. Materials and methods:Thirty healthy male adult albino rats were classified into Control group (Group I). Fluoxetine treated (Group II) received 24 mg/kg/day of fluoxetine dissolved in 1.0 mL of tap water once a day. Fluoxetine group treated with ADSCs (Group III) received fluoxetine as group (II) for 30 days and then was injected once by ADSCs at a dose of 1 × 106 cells/rat in the tail vein suspended in 0.5 ml of phosphate-buffered saline (PBS). Recovery group (Group IV) received fluoxetine for 30 days and then received no treatment till the end of the experiment. Results: The ultrastructural observations of the fluoxetine-treated group revealed major histological changes in both the pars distalis and nervosa. Pars distalis revealed cells with different shapes, sizes, nuclei, and variable profiles of the cytoplasm. Pars nervosa, on the other hand, revealed pituicytes with electron-lucent cytoplasm and small apoptotic nuclei. Administration of ADSCs greatly improved the microscopic appearance of cells, while the recovery group showed similar histological changes as the fluoxetine group. Conclusion: Fluoxetine caused various deleterious changes in the pituitary gland of albino rats, as evidenced by electron microscopy. These changes were almost corrected by the ADSCs treatment. .","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 1","pages":"334 - 347"},"PeriodicalIF":1.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45886887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of Vitamin D on testosterone and uncarboxylated osteocalcin levels in aged male rats 维生素D对老年雄性大鼠睾酮和未羧化骨钙素水平的影响

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-06-08 DOI: 10.1080/01913123.2022.2083280

G. Coşkun, L. Sencar, A. Tuli, Ebru Dundar Yenilmez, S. Polat

{"title":"The effect of Vitamin D on testosterone and uncarboxylated osteocalcin levels in aged male rats","authors":"G. Coşkun, L. Sencar, A. Tuli, Ebru Dundar Yenilmez, S. Polat","doi":"10.1080/01913123.2022.2083280","DOIUrl":"https://doi.org/10.1080/01913123.2022.2083280","url":null,"abstract":"ABSTRACT There is a well-established complex interaction between vitamin D metabolism and bone and gonad functions. In this study, we aimed to investigate the potential effects of vitamin D therapy on testosterone and osteocalcin (OC) levels in aged male rats. Forty-five adult male rats were divided into three groups in this study. Unlike the control group, the two experimental groups received 50 IU/kg/day and 100 IU/kg/day of vitamin D3 (cholecalciferol), respectively, for a 4-week period using the gavage method. Testicular tissue and blood samples from rats were collected under general anesthesia at the end of the 4-week period. Testicular tissue samples were examined using light and electron microscopy. Additionally, serum testosterone and OC levels were measured in blood samples. The 50 IU/kg dose of cholecalciferol increased testosterone and OC levels, which were lower than normal due to aging, and regulated the organization of the seminiferous tubule epithelium and interstitium more effectively than the 100 IU/kg dose of cholecalciferol. Male fertility functions and bone health, which degrade due to aging, were increased due to the use of exogenous vitamin D, although the higher dose was not associated with more effective results.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 1","pages":"368 - 376"},"PeriodicalIF":1.0,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44866591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the ultrastructural and viscoelastic characteristics of whole blood after exposure to the heavy metals cadmium, lead and chromium, alone and in combination 研究镉、铅、铬等重金属单独及联合暴露后全血的超微结构和粘弹性特性

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2022-05-15 DOI: 10.1080/01913123.2022.2075999

L. Pretorius, H. Taute, M. van Rooy, H. Oberholzer

{"title":"Investigating the ultrastructural and viscoelastic characteristics of whole blood after exposure to the heavy metals cadmium, lead and chromium, alone and in combination","authors":"L. Pretorius, H. Taute, M. van Rooy, H. Oberholzer","doi":"10.1080/01913123.2022.2075999","DOIUrl":"https://doi.org/10.1080/01913123.2022.2075999","url":null,"abstract":"ABSTRACT In recent years, heavy metal exposure has become a serious health concern as more humans are being exposed to heavy metals on a daily basis. Most of the environmental contamination and human exposure result from anthropogenic activities such as mining and smelting. The industrial and agricultural sectors also play a big role. Cigarette smoke in particular contains trace amounts of heavy metals that put chronic smokers at serious risk. Previous studies have determined that there is a strong correlation between heavy metal exposure and adverse effects on the coagulation system. The aim of this study was to investigate the effect of cadmium, lead and chromium alone and in combination on erythrocytes and fibrin networks that form part of the coagulation system as well as the viscoelastic properties of thrombus formation by using thromboelastography®. The choice of metals for this study was based on a previous study that compared the levels of metals between smokers and nonsmokers and found significantly higher levels of cadmium, lead and chromium in the platelet-rich fibrin of smoking individuals. Scanning electron microscopy analysis revealed that the cadmium and chromium combination groups caused the highest degree of echinocyte formation and fibrin network alterations. These findings were supported by the thromboelastography® analysis that indicated a significant decrease in reaction-time and split point values for the chromium-containing group, suggesting a shorter initiation time for clot formation. The findings of this study support the hypothesis that the coagulation pathway is a potential target for heavy metal toxicity.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"46 1","pages":"323 - 333"},"PeriodicalIF":1.0,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49148864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1