The impact of busulfan on the testicular structure in prepubertal rats: A histological, ultrastructural and immunohistochemical study.

IF 1.1 4区 医学 Q4 MICROSCOPY
Reem Ibrahim Abd El-Hay, Walaa H E Hamed, Nesreen Mostafa Omar, Dalia Refat El-Bassouny, Salwa A Gawish
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引用次数: 0

Abstract

Busulfan is a widely used cancer chemotherapeutic agent. Temporary or permanent sterility in male patients is one of the most common side effects of this drug. The present study was performed to evaluate the changes in the microscopic structure of the testes of prepubertal rats, as well as the changes in PCNA and caspase-3 immune expression, at different durations after busulfan administration. The rats were 5 weeks old and were divided into two main groups. Control group and busulfan treated group. Busulfan treated group received a single dose of busulfan (40 mg/kg), then animals were subdivided to three subgroups; IIa, IIb, IIc which were sacrificed after four, ten and twenty weeks, respectively, from the beginning of the experiment. Light and electron microscopic studies were done. Serum testosterone level and relative testes weight were assessed. Immunohistochemical staining for anti-proliferating cell nuclear antigen (PCNA) and anti-caspase-3 antigen was also done. Morphometric and statistical studies were carried out. Group II revealed histological and ultrastructural degenerative changes including congested blood vessels and degenerated spermatogenic epithelium, Sertoli cells, and Leydig cells. These changes were more evident after 10 weeks of busulfan administration and were accompanied by absence of mature sperms in the lumen of seminiferous tubules. These changes were associated with a significant reduction in relative testes weight, testosterone level, germinal epithelial height and seminiferous tubule diameter. Moreover, PCNA and caspase-3 immune expression was significantly altered in busulfan treated group. Mild improvement in testicular structure was observed 20 weeks after busulfan treatment.

丁硫丹对青春期前大鼠睾丸结构的影响:组织学、超微结构和免疫组织化学研究。
布磺胺是一种广泛使用的癌症化疗药物。男性患者的暂时性或永久性不育是这种药物最常见的副作用之一。本研究旨在评价布苏凡给药后不同时间青春期前大鼠睾丸显微结构的变化,以及PCNA和caspase-3免疫表达的变化。这些大鼠均为5周龄,被分为两组。对照组和丁硫凡治疗组。丁硫丹治疗组给予单剂量丁硫丹(40 mg/kg),再将动物分为3个亚组;IIa、IIb、IIc分别于实验开始后4周、10周、20周后处死。进行了光镜和电镜研究。测定血清睾酮水平和相对睾丸重量。同时进行抗增殖细胞核抗原(PCNA)和抗caspase-3抗原的免疫组化染色。进行了形态计量学和统计学研究。II组表现为组织学和超微结构的退行性改变,包括血管充血,生精上皮、支持细胞和间质细胞退化。这些变化在服用10周后更为明显,并伴有精管腔内成熟精子的缺失。这些变化与睾丸相对重量、睾酮水平、生发上皮高度和精小管直径的显著降低有关。此外,丁硫凡治疗组PCNA和caspase-3免疫表达显著改变。布苏凡治疗20周后,睾丸结构有轻度改善。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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