Ultrastructural Pathology最新文献_第2页

Histological study of Astaxanthin's neuroprotective potential in alleviating cisplatin-induced cerebellar cortex damage in adult male rats. 虾青素减轻顺铂诱导的成年雄性大鼠小脑皮质损伤的神经保护作用的组织学研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2025-10-25 DOI: 10.1080/01913123.2025.2579690

Eman Ali Elkordy, Amal Ahmed El-Sheikh

{"title":"Histological study of Astaxanthin's neuroprotective potential in alleviating cisplatin-induced cerebellar cortex damage in adult male rats.","authors":"Eman Ali Elkordy, Amal Ahmed El-Sheikh","doi":"10.1080/01913123.2025.2579690","DOIUrl":"10.1080/01913123.2025.2579690","url":null,"abstract":"Cisplatin (CIS) is a widely used chemotherapeutic agent with proven efficacy against various malignancies; however, its clinical utility is often constrained by dose-dependent neurotoxicity. This study investigated the histopathological, ultrastructural, and biochemical effects of astaxanthin (AST), a potent antioxidant, on the cerebellar cortex in a rat model of CIS-induced neurotoxicity. Twenty-four adult male Sprague Dawley rats were randomly assigned to four groups: Control, AST, CIS, and AST + CIS. CIS was administered intraperitoneally (10 mg/kg), while AST was given orally (20 mg/kg) for 10 consecutive days. CIS administration induced marked alterations in the cerebellar cortex, including Purkinje cell shrinkage, dendritic loss, and neuronal vacuolation, accompanied by reduced glutathione (GSH) and elevated malondialdehyde (MDA) levels, indicating oxidative stress. Co-treatment of CIS with AST significantly ameliorated these changes, preserving cerebellar architecture, Purkinje cell morphology, and restoring antioxidant balance. These findings suggest that AST exerts neuroprotection against CIS-induced cerebellar toxicity, likely through its antioxidant and anti-apoptotic properties. Further studies are warranted to clarify the underlying molecular pathways and evaluate AST potential as an adjuvant in CIS-based chemotherapy.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"11-27"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bruce Mackay, MD, PhD, founding member of Society for Ultrastructural pathology: in memoriam - his legacy lives on. 布鲁斯·麦凯，医学博士，超微结构病理学学会创始成员：纪念-他的遗产将永存。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-01-06 DOI: 10.1080/01913123.2025.2610867

John Hicks, Josep Lloreta-Trull

引用次数: 0

The role of mast cells in simvastatin-induced myopathy and the possible protective role of omega-3 fatty acids in adult male albino rats: light and electron microscopic study. 肥大细胞在辛伐他汀诱导的肌病中的作用以及成年雄性白化大鼠omega-3脂肪酸可能的保护作用：光镜和电镜研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-04-14 DOI: 10.1080/01913123.2026.2657294

Dalia Ahmed Baha Eldein, Shereen Hamed, Shireen Mazroa, Amal Mohamed Moustafa

{"title":"The role of mast cells in simvastatin-induced myopathy and the possible protective role of omega-3 fatty acids in adult male albino rats: light and electron microscopic study.","authors":"Dalia Ahmed Baha Eldein, Shereen Hamed, Shireen Mazroa, Amal Mohamed Moustafa","doi":"10.1080/01913123.2026.2657294","DOIUrl":"10.1080/01913123.2026.2657294","url":null,"abstract":"Simvastatin is antihyperlipidemic drugs that inhibit hydroxy methylglutaryl CoA reductase to decrease cholesterol synthesis; however, it causes skeletal myopathy. Omega-3 are polyunsaturated fatty acids with anti-inflammatory and anti-oxidant effects. Mast cells have an important role in the response to tissue injury by secreting different mediators and cytokines. The current study was performed to assess the role of mast cells in simvastatin-induced myopathy and the possible protective role of omega-3 fatty acids. Forty adult male albino rats were divided into four main groups received the drugs orally for of 21 days. Group 1: 1 ml distilled water, group 2: 300 mg/kg/day omega-3, group 3: 88 mg/kg/day simvastatin, and group 4: 88 mg/kg/day simvastatin plus 300 mg/kg/day omega-3. Light and electron microscopic studies were done along with morphometric and statistical studies. Group 1 and 2 showed regular histological structure. By light microscope, group 3 revealed muscle fiber disorganization, splitting, loss of striations, and cytoplasmic fragmentation. Some nuclei were centrally displaced. Toluidine blue sections showed a large number of mast cells. By transmission electron microscope, there was loss of the regular banding pattern with irregular mitochondria, nuclei, and sarcolemma. The concurrent administration of simvastatin with omega-3 showed less myopathic changes, decrease in mast cell numbers and more activation of the satellite stem cells to accomplish the process of muscle regeneration. In conclusion, the administration of omega-3 can decrease the myopathic changes of simvastatin. Further studies are recommended to explore the mast cells' role in the progression and repair of statin-induced myopathy.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"176-195"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deposits of C3 glomerulopathies have ultrastructural hints to correlate with immunofluorescent findings. C3肾小球病变的沉积具有与免疫荧光相关的超微结构提示。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-06-02 DOI: 10.1080/01913123.2026.2682586

Casey P Schukow, Neal B Blatt, Hassan D Kanaan, Larysa T Wickman, Brooke A Blazius, Radhika K Thalla, Wei Li, Marrouf Azar, Joanna Q Sattar, Saima S Mansuri, Jamal Abukhaled, Ping L Zhang

{"title":"Deposits of C3 glomerulopathies have ultrastructural hints to correlate with immunofluorescent findings.","authors":"Casey P Schukow, Neal B Blatt, Hassan D Kanaan, Larysa T Wickman, Brooke A Blazius, Radhika K Thalla, Wei Li, Marrouf Azar, Joanna Q Sattar, Saima S Mansuri, Jamal Abukhaled, Ping L Zhang","doi":"10.1080/01913123.2026.2682586","DOIUrl":"10.1080/01913123.2026.2682586","url":null,"abstract":"Background: C3 glomerulopathy (C3G) is a rare type of glomerular disease characterized by predominant deposits of C3 complement. Although dominant C3 immunofluorescent (IF) staining has become well known as a diagnostic criterion, the findings of electron microscopy (EM) are not well specified. The goal of this study was to scrutinize the characteristic features of C3 deposits on EM and to correlate with C3 IF staining patterns.Methods: We examined the EM images of a cohort of 24 cases of C3G (22 C3 dominant glomerulonephritis [C3GN] and 2 dense deposit disease [DDD]) to determine their characteristic features when compared to a cohort of patients with immune complex-mediated glomerulonephritis.Results: In our patients with C3G, the C3 deposits were present in all three glomerular compartments (mesangial, subendothelial, and subepithelial spaces) and they had the following features: (1) Smear pattern of C3 deposits along glomerular basement membranes (GBM) and subepithelial spaces; (2) C3 deposits were mostly lighter in gray colors as opposed to the dark black appearance of immune complex deposits (ICD); (3) C3 aggregates revealed smooth contours with a homogeneous fine granular appearance when compared to the humpy and bumpy appearance of ICD, and (4) C3 deposits rarely showed either retraction artifacts around the deposits or vacuolization within the GBM or mesangial areas.Conclusions: In our cohort, C3 deposits exhibit reproducible EM features that are well correlated with clinical data and dominant C3 staining by IF and are different from ICD upon securitized review.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"321-328"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148144949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural differences in the myocardium of cats with dilated cardiomyopathy eating high- versus low-pulse diets. 扩张型心肌病猫食用高脉冲与低脉冲饮食的心肌超微结构差异。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-04-29 DOI: 10.1080/01913123.2026.2664172

Mark D Bilger, Brian R Berridge, Gregory M Hendricks, Esther Gisela Martinez-Romero, Richard N Mitchell, Keith R Reddig, John E Rush, Lisa M Freeman

{"title":"Ultrastructural differences in the myocardium of cats with dilated cardiomyopathy eating high- versus low-pulse diets.","authors":"Mark D Bilger, Brian R Berridge, Gregory M Hendricks, Esther Gisela Martinez-Romero, Richard N Mitchell, Keith R Reddig, John E Rush, Lisa M Freeman","doi":"10.1080/01913123.2026.2664172","DOIUrl":"10.1080/01913123.2026.2664172","url":null,"abstract":"In 2018, the FDA issued an alert about a potential link between dilated cardiomyopathy (DCM) in pet dogs and cats and diets high in pulses (peas, lentils, chickpeas, and beans). Given reported myocardial ultrastructural abnormalities in this disease, the objective was to compare myocardial mitochondria, autophagic structures, and other subcellular organelles in cats with DCM eating high- versus low-pulse diets. Left ventricular tissue from cats with DCM eating high- (n = 4) or low-pulse (n = 3) diets was evaluated using transmission electron microscopy. Myofibrils, mitochondria, T-tubules, nuclei, z-lines, autophagic structures, and intercalated discs were evaluated qualitatively. Diet groups were compared for qualitative findings, as well as mitochondrial number, area, and perimeter; sarcomere length; lipid droplet number and area, and mitochondrial stage in the perinuclear, interfibrillar, and subsarcolemmal regions. Cats with DCM eating high-pulse diets had more unknown vacuoles and fewer Stage B mitochondria, with more Stage BC mitochondria compared to cats with DCM eating low-pulse diets. These results suggest that cats with DCM eating high-pulse diets have mitochondria in more advanced stages of degeneration. The higher number of unknown vacuoles could represent more mitochondrial degeneration or increased autophagy. Further ultrastructural investigation is warranted to help identify the cause of this diet-associated disease.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"196-205"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Granulocyte-colony stimulating factor protection against tramadol-induced changes in the adrenal cortex of adult male albino rats: histology, immunohistology, endocrine, and ultrastructure aspects. 粒细胞集落刺激因子对曲马多诱导成年雄性白化大鼠肾上腺皮质变化的保护作用：组织学、免疫组织学、内分泌和超微结构方面。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2025-11-07 DOI: 10.1080/01913123.2025.2584119

Shaymaa Hussein Hasan Ahmed, Hoda Atef Mohamed, Ghalia Mahfouz Attia Attia, Dalia Refat Mohamed El-Bassouny

{"title":"Granulocyte-colony stimulating factor protection against tramadol-induced changes in the adrenal cortex of adult male albino rats: histology, immunohistology, endocrine, and ultrastructure aspects.","authors":"Shaymaa Hussein Hasan Ahmed, Hoda Atef Mohamed, Ghalia Mahfouz Attia Attia, Dalia Refat Mohamed El-Bassouny","doi":"10.1080/01913123.2025.2584119","DOIUrl":"10.1080/01913123.2025.2584119","url":null,"abstract":"Tramadol (TRM) is a centrally acting analgesic drug used for management of moderate to severe pain. Granulocyte colony-stimulating factor (G-CSF) is a cytokine that has the ability to mobilize stem cells from the bone marrow to the peripheral circulation. This study was performed to evaluate the histological and biochemical alterations in the adrenal cortex after intake of tramadol and the possible protective role of G-CSF on it. Fifty adult male albino rats were divided into three groups: Group I as a control group, group II (TRM treated group) received a daily dose of 80 mg/kg body weight orally via gastric tube for 12 weeks and group III (TRM+G-CSF-treated group) received subcutaneous injections of 100 μg/kg body weight of G-CSF for seven consecutive days, then TRM from the 8th day to the end of the experiment in the same dose as group II. At the end of the experiment, blood samples were taken for hormonal essay and tissue samples were processed. Light and electron microscopic studies were done. Morphometric and statistical studies were carried out. The study revealed that TRM induced histological and ultrastructural degenerative changes, decreased serum levels of aldosterone, cortisol, and dehydroepiandrosterone, as well as a strong positive Bax immune reaction. However, G-CSF reversed these alterations and showed a strong positive CD34 immune reaction. In conclusion: G-CSF improved histological, biochemical and immunohistochemical metrics in the rat adrenal cortex after tramadol-induced injury.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"56-81"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145472226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is formalin-fixed paraffin embedded tissue good enough for studies of transmission electron microscopy of tumors? a comparative study with wet tissue. 福尔马林固定石蜡包埋组织是否足以用于肿瘤的透射电镜研究？湿组织的比较研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-01-04 DOI: 10.1080/01913123.2025.2612475

Katia Gabriela Chan Guzmán, María Del Pilar Ramos-Godínez, Guillermo Ernesto Corredor-Alonso, Hugo Ricardo Domínguez-Malagón

{"title":"Is formalin-fixed paraffin embedded tissue good enough for studies of transmission electron microscopy of tumors? a comparative study with wet tissue.","authors":"Katia Gabriela Chan Guzmán, María Del Pilar Ramos-Godínez, Guillermo Ernesto Corredor-Alonso, Hugo Ricardo Domínguez-Malagón","doi":"10.1080/01913123.2025.2612475","DOIUrl":"10.1080/01913123.2025.2612475","url":null,"abstract":"Transmission electron microscopy (TEM) has historically been pivotal in diagnosing neoplasms with challenging histopathology. However, its use has declined due to cost and the advent of ancillary techniques like immunohistochemistry. This study compares the diagnostic utility of formalin-fixed paraffin-embedded (FFPE) tissue versus wet glutaraldehyde-paraformaldehyde fixed tissue (WT) for TEM in neoplasms. A retrospective analysis of 746 cases (2014-2023) from the National Cancer Institute of Mexico - Instituto Nacional de Cancerología de Mexico (INCan) was performed. Cases were divided into Group A (FFPE, n = 270) and Group B (WT, n = 476). Diagnostic utility was assessed based on ultrastructural preservation and concordance with histopathological reports. Key ultrastructural features, such as microvilli in mesothelioma, cytoplasmic organelles in chondroblastoma, luminal microvilli in secretory breast carcinoma, continuous basal lamina in schwannoma, and abundant rough endoplasmic reticulum in fibroblastic tumors were identifiable in both groups despite artifacts in FFPE. Exact Fisher's test showed no significant difference in diagnostic utility between groups (p = .0548), with 87.03% (235/270) of FFPE cases and 88.66% (422/476) of WT deemed diagnostically useful. Specificity for WT was 100% versus comparable values for FFPE. This study challenges the notion that paraffin embedding severely compromises TEM diagnostic value. FFPE allows precise sampling of archived tissues, expanding TEM's applicability in retrospective studies. Further validation is warranted to confirm these findings.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"107-113"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145901180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration of adult neural stem cells by gold nanoparticles in a demyelination model induced in the hippocampus of adult male mice: a structural and ultrastructural study. 金纳米颗粒对成年雄性小鼠海马脱髓鞘模型中成体神经干细胞的改变：结构和超微结构研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-06-16 DOI: 10.1080/01913123.2026.2688836

Nora M Hamada, Mona Fm Soliman, Amal Mohamed Moustafa, Shireen A Mazroa

{"title":"Alteration of adult neural stem cells by gold nanoparticles in a demyelination model induced in the hippocampus of adult male mice: a structural and ultrastructural study.","authors":"Nora M Hamada, Mona Fm Soliman, Amal Mohamed Moustafa, Shireen A Mazroa","doi":"10.1080/01913123.2026.2688836","DOIUrl":"10.1080/01913123.2026.2688836","url":null,"abstract":"Adult neural stem cells (NSCs) are located in the sub-granular zone (SGZ) of the dentate gyrus (DG) of the hippocampus, one of the prominent regions of neurogenesis in the adult brain. Activation of endogenous stem cells may provide a therapeutic approach in demyelinating diseases. Due to their minuscule size, nanoparticles (AuNPs) can cross the blood-brain barrier. To evaluate the effect of AuNPs on adult NSCs and the progression of demyelination in the hippocampus in a model of demyelination, thirty-two adult mice were divided into: control group I, AuNPs group II (10 mg/kg BW/day), cuprizone (CPZ) group III (0.2% orally for 8 weeks to induce demyelination), and CPZ & AuNPs group IV. Hippocampus was prepared for light microscopy, nestin immunohistochemical staining (NSCs' immune marker), and electron microscopy. In groups I and II, spindle-shaped adult NSCs in the SGZ of DG showed a positive nestin immunoreaction. In group III, most of the nerves were demyelinated. Pyramidal and granular neurons showed degenerative changes. Nestin-immune-positive cells were significantly decreased. In group IV, preservation of the myelin, less degenerative changes, and a significant increase in nestin-positive cells were detected. In conclusion, AuNPs activated adult NSCs in the mouse hippocampus, partially ameliorated degenerative changes, and enhanced partial remyelination in the CPZ demyelination model.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"367-384"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148266259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental fibrosis and microvascular dysfunction in gestational diabetes mellitus: histopathological and ultrastructural evidence from a South African cohort. 妊娠期糖尿病的胎盘纤维化和微血管功能障碍：来自南非队列的组织病理学和超微结构证据。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-07-22 DOI: 10.1080/01913123.2026.2706777

Elizabeth Rossenrode, Ashwill van Tonder, Isabella Lohse, Felicia Masetla, Sumaiya Adam, Hester Magdalena Oberholzer, Mia-Jeanne van Rooy

{"title":"Placental fibrosis and microvascular dysfunction in gestational diabetes mellitus: histopathological and ultrastructural evidence from a South African cohort.","authors":"Elizabeth Rossenrode, Ashwill van Tonder, Isabella Lohse, Felicia Masetla, Sumaiya Adam, Hester Magdalena Oberholzer, Mia-Jeanne van Rooy","doi":"10.1080/01913123.2026.2706777","DOIUrl":"10.1080/01913123.2026.2706777","url":null,"abstract":"Gestational diabetes mellitus (GDM) is a pregnancy-associated metabolic disorder characterized by hyperglycemia and chronic low-grade inflammation, both contributing to placental dysfunction. This study investigated placental pathology in a South African cohort. GDM placentas demonstrated increased fibrin deposition and altered villous stromal architecture, consistent with a stressed placental microenvironment. Polarized light microscopy revealed heterogeneous collagen birefringence patterns, indicating disrupted extracellular matrix organization with a shift in type I and type III collagen balance, consistent with progressive fibrotic remodeling and impaired microvascular integrity. Ultrastructural examination revealed thickened basement membranes and mitochondrial degeneration, indicative of microangiopathic injury and oxidative stress-related cellular damage. These findings demonstrate coordinated histopathological and ultrastructural placental remodeling in GDM involving inflammation, fibrosis, and microvascular dysfunction.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"394-408"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148563115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential neuroprotective effect of apigenin on cerebellar cortex alterations in valproic acid-induced autism model of male albino rat pups: a histological and immunohistochemical study. 芹菜素对丙戊酸诱导的雄性白化大鼠幼鼠自闭症模型小脑皮质改变的潜在神经保护作用：组织学和免疫组织化学研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-06-11 DOI: 10.1080/01913123.2026.2682585

Shaimaa Mostafa Kashef, Hend Ibrahim Abd El Halim, Maram Mohammed El Tabaa, Manar Mohammed El Tabaa, Maram Mohamed Elkelany

{"title":"The potential neuroprotective effect of apigenin on cerebellar cortex alterations in valproic acid-induced autism model of male albino rat pups: a histological and immunohistochemical study.","authors":"Shaimaa Mostafa Kashef, Hend Ibrahim Abd El Halim, Maram Mohammed El Tabaa, Manar Mohammed El Tabaa, Maram Mohamed Elkelany","doi":"10.1080/01913123.2026.2682585","DOIUrl":"10.1080/01913123.2026.2682585","url":null,"abstract":"Autism is a neurodevelopmental condition with challenges in establishing and sustaining social relationships and communication. Apigenin (AP) is a natural flavonoid with numerous valuable effects on brain disorders. The current study emerged to study the cerebellar histopathological changes in valproic acid (VPA)-induced autism in male albino rat pups and assess the possible neuroprotective role of AP in minimizing these alterations. Forty male albino rat pups were categorized randomly into four equal groups: control group, AP group administered AP (250 mg/kg/day orally) from postnatal days (PND)14 to PND 40, VPA group received single subcutaneous injection of VPA (400 mg/kg) on PND14 and VPA+AP group were injected with VPA, then received AP at the identical dosage and approach as the preceding groups. Shrunken Purkinje cells with dark heterochromatic nuclei, neuropil vacuolation, and myelin sheath deformities were reported on VPA administration. A significant rise in cerebellar immune expression of GFAP, p-Tau and nestin. Also, a significant reduction in the duration of sniffing, line crossing, and rearing was recorded. Additionally, a decline of glutathione peroxidase, serotonin and the rise of malondialdehyde and IL-1β levels were recorded in cerebellar homogenate. The co-treatment of AP with VPA significantly rescued these histological alterations and reinstated antioxidant equilibrium.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"283-306"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148228401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0