Ultrastructural Pathology最新文献_第3页

Unconventional nucleus export and secondary necrosis of human leukemia T lymphocytes (Jurkat cells) transfected with HIV-1 LTR CAT-TAT-72: ultrastructural assessment. 转染HIV-1 LTR CAT-TAT-72的人白血病T淋巴细胞（Jurkat细胞）的非常规核输出和继发性坏死：超微结构评价

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-08-31 DOI: 10.1080/01913123.2025.2545858

Jacques Gilloteaux, Etienne de Harven, John Koch

{"title":"Unconventional nucleus export and secondary necrosis of human leukemia T lymphocytes (Jurkat cells) transfected with HIV-1 LTR CAT-TAT-72: ultrastructural assessment.","authors":"Jacques Gilloteaux, Etienne de Harven, John Koch","doi":"10.1080/01913123.2025.2545858","DOIUrl":"https://doi.org/10.1080/01913123.2025.2545858","url":null,"abstract":"Efficient transcriptional activation and replication of the human immunodeficiency virus (HIV-1) is dependent on Tat protein. Initial observations have shown that human leukemia T lymphocytes (Jurkat cells aka Wild type or WT) transfected with HIV-1 LTR CAT TAT-72 plasmid as Control (CTJ) cells, and CTJ transfected by electroporation with pcDNA3-pU3R-CAT-TAT-72 (TJ cells) showed growth and maintenance resulting in giant and small cells with accumulated corpses. The lack of fine structure in Jurkat cells and both transfected cells aimed at us to verify their respective ultrastructure modifications. Scanning and transmission electron microscopy showed evident Cajal bodies in CTJ cells compared with WT cells and revealed unconventional nucleus export of viral-associated transcripts where eruption of the nucleus envelope spilled content from the perinuclear space toward exocytosis via a lined membranous channel continuum of endoplasmic reticulum - mitochondria envelope. TJ cells grew as small and giant cells where giant cells bore virological synapses stimulating apoptogenic impetus but where cell demise included osmotic bursts mostly resulted into secondary necrosis. Jurkat transfected cells could model in vitro resilient human T lymphocytes (or other infected cells) that remain factories of expressed HIV-1 virus proteins such as Tat, secreted and captured by other cells as in viral infections and can form giant cells. Those Tat and other transcripts induced cell demise, as in vivo those HIV-1 viral proteins and traces can amplify infectivity as in AIDS, altering primary lymphoid organs and secondary lymphoid organs.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 5","pages":"421-448"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malignant glomus tumor: a rare case with an ultrastructural study. 恶性血管球瘤：罕见病例的超微结构研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-30 DOI: 10.1080/01913123.2025.2499140

Corredor-Alonso Guillermo Ernesto, Arredondo-Ruiz Pedro, Martinez-Tlahuel Jorge Luis, Dominguez-Malagon Hugo Ricardo

引用次数: 0

Which has a more protective effect against neuronal changes in the cingulate cortex of newborn rats of diabetic mothers: omega 3 or glutathione? 对于糖尿病母亲的新生大鼠，欧米茄3和谷胱甘肽哪一种对其扣带皮层神经元变化的保护作用更强？

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-11 DOI: 10.1080/01913123.2025.2490161

Esraa Nafadi, Walaa Farag

{"title":"Which has a more protective effect against neuronal changes in the cingulate cortex of newborn rats of diabetic mothers: omega 3 or glutathione?","authors":"Esraa Nafadi, Walaa Farag","doi":"10.1080/01913123.2025.2490161","DOIUrl":"https://doi.org/10.1080/01913123.2025.2490161","url":null,"abstract":"Different researches suggests that unchecked maternal hyperglycemia during pregnancy may negatively impact the offspring's neurodevelopment. Omega 3 polyunsaturated fatty acids are vital antioxidant micronutrients for neurological health. Glutathione (GSH) is an essential non-enzymatic antioxidant in mammalian cells. The study aimed to assess the possible ameliorative effect of omega3 versus GSH against the neuronal changes in the cingulate cortex of new born rats of diabetic mothers. Fifty female rats were divided randomly into 5 equal groups: Group I given saline, group II where induction of diabetes by single intraperitoneal injection of 150 mg/kg of alloxan monohydrate, group III given oral omega 3 at a dose of 300 mg/kg, group IV given intraperitoneal injections of GSH at a dose of (200 mg/kg) and group V given both omega 3 and GSH with the same routes and regimens as group III and IV throughout the whole pregnancy. The newborn of each group was collected and anaesthetized then brain specimens were extracted and processed for the light, immunohistochemical and electron microscopic studies. Group II showed marked degenerative changes in the all layers of cingulate cortex by light and electron microscopy. The cingulate cortex of group V showed the most degree of improvement in comparison to group III and IV. There was strong caspase3 immunoreaction in group II while weak reaction appeared with group III, IV and V. We concluded that the combined administration of omega 3 and GSH mitigate the adverse effects of maternal diabetes on the newborn's cingulate cortex.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"243-256"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent trends in the causation of peritoneal mesothelioma: fiber burden analysis of ten cases. 腹膜间皮瘤病因的最新趋势：10例纤维负荷分析。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-03-26 DOI: 10.1080/01913123.2025.2483226

Victor L Roggli, Stasha Novakovic, Andrew J Ghio, Huihua Li, Sergio Pina-Oviedo, John M Carney, Thomas A Sporn, Carolyn H Glass, Elizabeth N Pavlisko

{"title":"Recent trends in the causation of peritoneal mesothelioma: fiber burden analysis of ten cases.","authors":"Victor L Roggli, Stasha Novakovic, Andrew J Ghio, Huihua Li, Sergio Pina-Oviedo, John M Carney, Thomas A Sporn, Carolyn H Glass, Elizabeth N Pavlisko","doi":"10.1080/01913123.2025.2483226","DOIUrl":"10.1080/01913123.2025.2483226","url":null,"abstract":"Mesothelioma in the past has been strongly associated with a history of asbestos exposure. Studies have shown that, on average, a higher dose of asbestos exposure is required for the development of peritoneal mesothelioma, and a smaller percentage of cases are asbestos related. Non-asbestos-related causes have been reported, including prior therapeutic radiation, genetic predisposition, and chronic inflammation (e.g. Crohn disease, endometriosis, ventriculo-peritoneal shunts, and diverticulitis). Cases in children have also been reported. Recent studies have shown a decreasing trend in fiber burdens and percentage of asbestos-related mesotheliomas, with similar observations in epidemiological studies. We performed fiber burden analysis on lung tissue in 10 cases (six men, four women) of peritoneal mesothelioma since 2010. Fiber analysis was performed using the sodium hypochlorite digestion technique, with asbestos body concentrations determined by light microscopy. Fiber concentrations and types were determined by scanning electron microscopy. The median age for the six men was 62 years (range: 53-75 years). Three cases were epithelioid type and three were biphasic. Two of six cases (33%) had an elevated lung fiber burden, with one case exclusively crocidolite and the other predominately amosite. The median age for the four women was 55 years (range: 39-63 years). Two cases were epithelioid type and two were biphasic. None of the four had an elevated lung fiber burden. Our findings are consistent with contemporary epidemiological studies indicating that a minority of peritoneal mesotheliomas occurring in men are asbestos related and very few are asbestos related in women.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"288-295"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient isolation and characterization of Serum-Derived Exosomes: evaluating ultracentrifugation and Total Exosome Isolation Reagent based precipitation. 血清衍生外泌体的高效分离和表征：评估超离心和基于沉淀的总外泌体分离试剂。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-25 DOI: 10.1080/01913123.2025.2507698

Ranjana Bharti, Munish Kumar, Veena Devi, Asha Rao, Ashish Aggarwal, Tulika Gupta

{"title":"Efficient isolation and characterization of Serum-Derived Exosomes: evaluating ultracentrifugation and Total Exosome Isolation Reagent based precipitation.","authors":"Ranjana Bharti, Munish Kumar, Veena Devi, Asha Rao, Ashish Aggarwal, Tulika Gupta","doi":"10.1080/01913123.2025.2507698","DOIUrl":"10.1080/01913123.2025.2507698","url":null,"abstract":"Exosomes are extracellular vesicles that carry biomolecular cargos such as proteins, lipids, RNA, and DNA. These molecules play crucial roles in cell-to-cell communication and are involved in various physiological and pathological processes. Due to their potential as biomarkers for disease diagnosis and prognosis, research has increasingly focused on developing more efficient methods for their isolation and characterization. In this study, blood samples were collected from 30 participants, and serum was subsequently isolated. Serum-derived exosomes (SDEs) were extracted using ultracentrifugation (UC) as well as the Total Exosome Isolation Reagent. Modifications to the UC method were implemented to improve yield and purity, and a detailed description of the method is also provided. The exosomes were characterized by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), and Western Blotting (WB) to evaluate their size, morphology, and protein content. The exosome yields from both isolation methods were evaluated using the BCA assay. Protein estimation suggested that the Total Exosome Isolation Reagent produced exosome concentrations that were 10-fold higher compared to those obtained through ultracentrifugation. Morphological analysis showed that exosomes exhibited circular, spherical, and irregular shapes, with diameters ranging from 30 to 200 nm. Western Blotting confirmed the presence of exosomal markers (TSG101, ALIX, LAMP2, and CD63) in the SDEs. In conclusion, both ultracentrifugation and the Total Exosome Isolation Reagent effectively isolate SDEs. Thus, although both methods are viable, modified ultracentrifugation is the preferred choice for applications due to its cost-effectiveness and suitability for achieving pure protein yields.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"340-353"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A primary renal neuroendocrine tumor presenting in a fifty-five-year-old woman: a report of a rare entity. 原发性肾神经内分泌肿瘤呈现在一个55岁的妇女：一个罕见的实体报告。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-06-18 DOI: 10.1080/01913123.2025.2520217

Rodney E Shackelford, Ardenne Martin, Charu Shastri, Muhammad Tahir, Guillermo A Herrera, Christopher E Keel, Carlina Medelaire

引用次数: 0

Protection against vancomycin-associated nephrotoxicity by zileuton: role of mitochondria revealed by electron microscopic study. zileuton对万古霉素相关肾毒性的保护作用：电镜研究揭示线粒体的作用。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-08-05 DOI: 10.1080/01913123.2025.2541667

Cole S Hudson, Yongqi Xiao, Vincent H Tam, Luan D Truong

{"title":"Protection against vancomycin-associated nephrotoxicity by zileuton: role of mitochondria revealed by electron microscopic study.","authors":"Cole S Hudson, Yongqi Xiao, Vincent H Tam, Luan D Truong","doi":"10.1080/01913123.2025.2541667","DOIUrl":"10.1080/01913123.2025.2541667","url":null,"abstract":"Vancomycin is commonly used to treat drug-resistant bacterial infections despite its potential to cause nephrotoxicity. We previously showed that zileuton can reduce vancomycin nephrotoxicity, but the intracellular mechanism(s) are not fully understood. The objective of this study was to improve our understanding of the mechanisms of nephroprotection by zileuton. Sprague-Dawley rats were administered vancomycin (200 mg/kg/day) and zileuton (4 mg/kg/day) for 3 days. Blood samples were collected to determine serum creatinine concentration. Kidneys were collected for morphologic study including comprehensive electron microscopic examination. Treatment with vancomycin alone resulted in doubling of serum creatinine. Significant tubular cell injury was noted by light microscopy. Electron microscopy further defined the nature of tubular cell injury including tubular cell necrosis, cytoplasmic vacuolization, endoplasmic reticulum dilation, and aggregation of granular/fibrillary material. Distinct mitochondrial changes included swelling, appearance of crowded/disorganized cristae, punctuated electron dense matrices, and large lysosomes containing substructures consistent with abnormal mitochondria. These changes were markedly attenuated with adjuvant zileuton. Our findings indicate that mitochondrial injury is a major mechanism of vancomycin-associated nephrotoxicity, and restoration of mitochondrial morphology is associated with nephroprotection by zileuton. These findings would be valuable for nephroprotective strategies to reduce vancomycin-associated nephrotoxicity, allowing optimal vancomycin use to treat bacterial infections.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"414-420"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diffuse intrapulmonary malignant mesothelioma (DIMM) mimicking interstitial lung disease. Ultrastructural analysis and the potential role of the Hedgehog signaling pathway. 模拟肺间质性疾病的弥漫性肺内恶性间皮瘤（DIMM）。超微结构分析及Hedgehog信号通路的潜在作用。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-08-23 DOI: 10.1080/01913123.2025.2549858

Hector Chavarria, Mohamed Zedan, Osama Elkadi, Kurt Knowles, Guillermo Herrera

{"title":"Diffuse intrapulmonary malignant mesothelioma (DIMM) mimicking interstitial lung disease. Ultrastructural analysis and the potential role of the Hedgehog signaling pathway.","authors":"Hector Chavarria, Mohamed Zedan, Osama Elkadi, Kurt Knowles, Guillermo Herrera","doi":"10.1080/01913123.2025.2549858","DOIUrl":"https://doi.org/10.1080/01913123.2025.2549858","url":null,"abstract":"Diffuse intrapulmonary malignant mesothelioma (DIMM) is a rare variant of epithelioid mesothelioma characterized by diffuse lung parenchymal involvement without a discrete pleural mass, often mimicking interstitial lung disease (ILD). We report a case of a 55-year-old male presenting with bilateral pleural effusions and a complex clinical course requiring multiple thoracic interventions. Histology revealed scattered nests of epithelioid tumor cells in the lung interstitium and lymph nodes, with immunohistochemistry positive for mesothelial markers (calretinin, WT1, D2-40) and negative for carcinoma markers. Ultrastructural analysis demonstrated classic mesothelial features including cohesive cells with desmosomes and abundant slender microvilli. next-generation sequencing showed no pathogenic mutations in commonly altered mesothelioma genes but revealed a variant of uncertain significance in PTCH1, implicating potential Hedgehog pathway involvement. This case underscores the diagnostic challenges of DIMM masquerading as ILD and highlights the value of integrating histopathology, ultrastructure, and molecular profiling to improve diagnostic accuracy and understand tumor biology.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 5","pages":"468-473"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-21 DOI: 10.1080/01913123.2025.2508065

引用次数: 0

Histological and biochemical evaluation of the effect of nandrolone decanoate on the prostate in postpubertal albino rats. 癸酸诺龙对青春期后白化大鼠前列腺影响的组织学和生化评价。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-07-21 DOI: 10.1080/01913123.2025.2531779

Heba M Abdel-Aziz, Amal S Abdel-Salam, Fayza E Ahmed, Abeer A Abdelrahman, Maha Z Mohammed

{"title":"Histological and biochemical evaluation of the effect of nandrolone decanoate on the prostate in postpubertal albino rats.","authors":"Heba M Abdel-Aziz, Amal S Abdel-Salam, Fayza E Ahmed, Abeer A Abdelrahman, Maha Z Mohammed","doi":"10.1080/01913123.2025.2531779","DOIUrl":"10.1080/01913123.2025.2531779","url":null,"abstract":"Nandrolone decanoate, a synthetic form of the testosterone hormone, is misused by some bodybuilders and adolescents to gain muscle mass. We investigated its effect on the prostate during postpubertal age and evaluated the reversibility of these effects after a period of cessation. Thirty albino rats were utilized and categorized into three distinct groups: Control group, a treated group that received nandrolone decanoate weekly by intramuscular injection with 10 mg/kg for 4 weeks while recovery group received nandrolone, similar to the treated group, followed by an additional recovery period of 4 weeks. In the treated group, there were significant elevation in IL-1β, IL-18, IL-6, TNF-α, COX2, MDA, BAX, PSA, and miRNA-155 expression and decreased in SOD, GST, bcl2, and testosterone hormone compared to the control group. Prostatic acini showed marked epithelial stratification, weak PAS positive reactions, excess collagen depositions, and negative ARs nuclear immunoexpression. Ultrastructrally, cytoplasm contained vacuoles and dilated cisterna of RER. In the recovery group, insignificant differences were observed between the recovery and control groups, and most of the acini appeared normal except for minimal changes. Nandrolone-induced structural and functional impairment of the prostate at cellular and molecular basis and cessation of it in the recovery group ameliorated the impairment.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"399-413"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0