Ultrastructural Pathology最新文献_第3页

Morphological and histopathological changes of maternal levetiracetam on the cerebellar cortex of the offspring of albino rat. 母体左乙拉西坦对白化大鼠后代小脑皮层的形态学和组织病理学变化

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-07-03 Epub Date: 2024-06-08 DOI: 10.1080/01913123.2024.2353064

Safaa M Hanafy

{"title":"Morphological and histopathological changes of maternal levetiracetam on the cerebellar cortex of the offspring of albino rat.","authors":"Safaa M Hanafy","doi":"10.1080/01913123.2024.2353064","DOIUrl":"10.1080/01913123.2024.2353064","url":null,"abstract":"Levetiracetam (LEV) is being used by women with reproductive-age epilepsy at a significantly higher rate. The purpose of the study was to assess how levetiracetam treatment during pregnancy affected the offspring's weight and cerebellum. Forty pregnant rats were divided into two groups (I, II). Two smaller groups (A, B) were created from each group. The rats in group I were gavaged with approximately 1.5 mL/day of distilled water either continuously during pregnancy (for subgroup IA) or continuously during pregnancy and 14 days postpartum (for subgroup IB). The rats in group II were gavaged with about 1.5 mL/day of distilled water (containing 36 mg levetiracetam) either continuously during pregnancy (for subgroup IA) or continuously during pregnancy and 14 days postpartum (for subgroup IB). After the work was completed, the body weight of the pups in each group was recorded, and their cerebella were analyzed histologically and morphometrically. Following levetiracetam treatment, the offspring showed decreased body weight and their cerebella displayed delayed development and pathological alterations. These alterations manifested as, differences in the thicknesses of the layers of cerebellar cortex as compared to the control groups; additionally, their cells displayed cytoplasmic vacuolation, nuclear alterations, fragmented rough endoplasmic reticulum and lost mitochondrial cristae. Giving levetiracetam to pregnant and lactating female rats had a negative impact on the body weight and cerebella of the offspring. Levetiracetam should be given with caution during pregnancy and lactation.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"247-260"},"PeriodicalIF":1.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection as a trigger of acute, transient glomerular deposition of clonal immunoglobulins. 感染是克隆免疫球蛋白急性、一过性肾小球沉积的诱因。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-07-03 Epub Date: 2024-05-16 DOI: 10.1080/01913123.2024.2356112

Bangchen Wang, Micah Schub, Derrick L Robinson, David N Howell

引用次数: 0

Hydralazine use can be associated with IgM-dominated immune complex-mediated glomerulonephritis. 使用氯丙嗪可能与 IgM 主导的免疫复合物介导的肾小球肾炎有关。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-07-03 Epub Date: 2024-04-29 DOI: 10.1080/01913123.2024.2346660

Ping L Zhang, Brandon D Metcalf, Sarang Khan, Jamal Abukhaled, Khalid Zafar, Wei Li, Hassan D Kanaan

{"title":"Hydralazine use can be associated with IgM-dominated immune complex-mediated glomerulonephritis.","authors":"Ping L Zhang, Brandon D Metcalf, Sarang Khan, Jamal Abukhaled, Khalid Zafar, Wei Li, Hassan D Kanaan","doi":"10.1080/01913123.2024.2346660","DOIUrl":"10.1080/01913123.2024.2346660","url":null,"abstract":"Context: IgM-dominant immune complex-mediated glomerulonephritis (IgM-dominant ICMGN) is a rare renal entity, characterized by a membranoproliferative pattern by light microscopy, dominant IgM staining by immunofluorescent staining, and subendothelial deposits by electron microscopy. This study was to investigate if some of IgM-ICMGN were associated with autoimmune disorders induced by hydralazine.Design: Seven IgM-dominant ICMGN cases were identified over 8 years. Their pathologic phenotypes and clinical scenarios were analyzed in detail.Results: Patients' ages ranged from 47 to 87 years old with 5 women and two men. Six of seven patients had drug-induced autoimmune phenomenon (hydralazine-induced positive ANCA and ANA). All of them had renal dysfunction and some proteinuria. Most pathologic features showed a membranoproliferative pattern of glomerulonephritis with dominant IgM deposits at subendothelial spaces. IgM nephropathy (a variant of focal segmental glomerulosclerosis), chronic thrombotic microangiopathy, and cryoglobulinemic glomerulopathy were ruled out in the cases.Conclusion: The hydralazine-induced autoimmune phenomenon can be seen in IgM-dominant ICMGN, which should be classified as a subtype of membranoproliferative glomerulonephritis.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"317-322"},"PeriodicalIF":1.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140857942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic liver fibrosis induction in aging causes significant ultra-structural deterioration in liver and alteration on immune response gene expressions in liver-spleen axis. 衰老诱导的慢性肝纤维化会导致肝脏超结构显著恶化，并改变肝脾轴心的免疫反应基因表达。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-07-03 Epub Date: 2024-06-06 DOI: 10.1080/01913123.2024.2360447

Zeynal Mete Karaca, Gamze Karaca, Başak Kayhan, Mehmet Gül, Veysel Ersan, Harika Gözükara Bağ, Elif Yeşilada

{"title":"Chronic liver fibrosis induction in aging causes significant ultra-structural deterioration in liver and alteration on immune response gene expressions in liver-spleen axis.","authors":"Zeynal Mete Karaca, Gamze Karaca, Başak Kayhan, Mehmet Gül, Veysel Ersan, Harika Gözükara Bağ, Elif Yeşilada","doi":"10.1080/01913123.2024.2360447","DOIUrl":"10.1080/01913123.2024.2360447","url":null,"abstract":"The relationship between damage to the liver and spleen by aging and the immune response status in these two organs, which are anatomically and immunologically interconnected, is unknown. The authors investigated the histopathological, ultrastructural, and immunological effects of aging in young and aged fibrotic mice by using an experimental model. Four groups were planned, with 10 mice in each experimental group. The levels of fibrosis and ultrastructural destruction in the liver were determined by α-SMA staining and TEM analysis. Expression levels of immunity genes (Il2, Il4, Il6, Il10, Il12, Il17, Tnf, Ifng, Tgfb1, Gata3, Rorc, Tbx21, Foxp3, Ccl2, Ccr2, Cxcr3, Pf4, Cxcl10) were carried out by qRT-PCR. While structural disorders were detected in the mitochondria of aged healthy group, cellular destruction in the fibrosis-induced elderly group was at a dramatic level. Fibrosis induction in aged mice caused an elevation in the expression of chemokines (CCl2, CXCL10, CCR2) and cytokine (IL-17a) genes that induce autoinflammatory response in the liver. Unlike the cellular pathology and genes activated in fibrosis in youth and the natural occurrence of fibrosis with aging, induction of fibrosis during aging causes deterioration in the liver and expression of genes responsible for autoimmunity in both the liver and spleen.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"261-273"},"PeriodicalIF":1.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141262983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential therapeutic effects of exosomes derived from bone marrow mesenchymal stem cells on ileum injury of a rat sepsis model (histological and immunohistochemical study). 骨髓间充质干细胞外泌体对败血症模型大鼠回肠损伤的潜在治疗作用（组织学和免疫组化研究）。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-07-03 Epub Date: 2024-07-01 DOI: 10.1080/01913123.2024.2368011

Heba M Elnegris, Abeer A Abdelrahman, Eman S El-Roghy

{"title":"The potential therapeutic effects of exosomes derived from bone marrow mesenchymal stem cells on ileum injury of a rat sepsis model (histological and immunohistochemical study).","authors":"Heba M Elnegris, Abeer A Abdelrahman, Eman S El-Roghy","doi":"10.1080/01913123.2024.2368011","DOIUrl":"https://doi.org/10.1080/01913123.2024.2368011","url":null,"abstract":"Sepsis denotes a serious high mortality concern. The study was designed to evaluate the effect of mesenchymal stem cell exosomes (MSC-exosomes) on the evolution of the animal model of sepsis. In this study, 36 rats were distributed into three groups, (I) controls, (II) LPS-treated, and (III) LPS+MSC-EVs. Sepsis was simulated by administering E. coli-LPS to the laboratory animals. Group III was given MSC-exosomes four hours after the LPS injection. Forty-eight hours later rats were sacrificed. Ileum samples were excised, and processed for the histological assessment, immunohistochemical identification of CD44, and inducible nitric oxide synthase (iNOS). Ileum homogenate was used to estimate tumor necrosis factor α (TNF α) besides Cyclooxygenase-2 (COX 2). PCR was used for the detection of interleukin 1α (IL‑1α), and interleukin 17 (IL‑17). Statistical and morphometrical analysis was done. The LPS-treated group showed increased TNF-α, IL‑1α, IL‑17, and decreased COX 2. LPS administration led to cytoplasmic vacuolization of enterocytes, an increase in the vasculature, and cellular infiltrations invaded the lamina propria. There was a significant rise in goblet cells and the proportion of collagen fibers. Ultrastructurally, the enterocytes displayed nuclear irregularity, rough endoplasmic reticulum (rER) dilatation, and increased mitochondria number. Sepsis induces a significant increase in iNOS and a decrease in CD44 immune expressions. LPS+MSC-EVs group restored normal ileum structure and revealed a significant elevation in CD44 and a reduction in iNOS immunoreactions. LPS-sepsis induced an obvious ileum inflammatory deterioration ameliorated by MSC-exosomes, mostly through their antioxidant, anti-inflammatory, and anti-apoptotic properties.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"48 4","pages":"274-296"},"PeriodicalIF":1.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling renal pathology's potential: exploring a rare subtype of amyloid - apolipoprotein CII amyloidosis in the youngest patient: a case report and literature review. 揭示肾脏病理学的潜力：探索一种罕见的淀粉样蛋白亚型--最年轻患者的载脂蛋白CII淀粉样变性：病例报告和文献综述。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-07-03 Epub Date: 2024-05-20 DOI: 10.1080/01913123.2024.2353397

Yiqin Zuo, Fiona Hanly, Duo Li, Efren Chavez, Omar Aljuboori, Gabriel Contreras, Guillermo A Herrera

引用次数: 0

Effect of peripheral blood mononuclear cells on ischemia-reperfusion injury of sciatic nerve of adult male albino rat: histological, immunohistochemical, and ultrastructural study. 外周血单核细胞对成年雄性白化大鼠坐骨神经缺血再灌注损伤的影响：组织学、免疫组织化学和超微结构研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2024-05-03 Epub Date: 2024-02-29 DOI: 10.1080/01913123.2024.2321144

Shaimaa Mostafa Kashef, Suzan Elsayed Abo Elnasr

{"title":"Effect of peripheral blood mononuclear cells on ischemia-reperfusion injury of sciatic nerve of adult male albino rat: histological, immunohistochemical, and ultrastructural study.","authors":"Shaimaa Mostafa Kashef, Suzan Elsayed Abo Elnasr","doi":"10.1080/01913123.2024.2321144","DOIUrl":"10.1080/01913123.2024.2321144","url":null,"abstract":"Ischemia/reperfusion (I/R) injury of sciatic nerve is a serious condition that results in nerve fiber degeneration, and reperfusion causes oxidative injury. Peripheral blood mononuclear cells (PBMNCs) have neuroregenerative power. This study was carried out to evaluate the potential ameliorative effect of PBMNCs on changes induced by I/R injury of the sciatic nerve. Fifty adult male albino rats were divided into donor and experimental groups that were subdivided into four groups: group I (control group), group II received 50 µL PBNMCs once intravenously via the tail vein, group III rubber tourniquet was placed around their Rt hind limb root for 2 hours to cause ischemia, group IV was subjected to limb ischemia as group III, then they were injected with 50 ul PBMNCs as group II before reperfusion. I/R injury showed disorganization of nerve fascicles with wide spaces in between nerve fibers. The mean area of collagen fibers, iNOS immunoexpression, and number of GFAP-positive Schwann cells of myelinated fibers showed a highly significant increase, while a highly significant reduction in the G-ratio and neurofilament immunoexpression was observed. Myelin splitting, invagination, evagination, and myelin figures were detected. PBMNC-treated group showed a marked improvement that was confirmed by histological, immunohistochemical, and ultrastructural findings.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"172-191"},"PeriodicalIF":1.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139991285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural differences between synovial sarcoma and solitary fibrous tumor: comparative study in adult patients from the National Cancer Institute of Mexico. 滑膜肉瘤与单发纤维瘤的超微结构差异：墨西哥国家癌症研究所成年患者的比较研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2024-05-03 Epub Date: 2024-02-13 DOI: 10.1080/01913123.2024.2313742

Guillermo Ernesto Corredor-Alonso, Leonardo Saúl Lino-Silva, Erika Yazmin López-Flores, Tatiana Velásquez-Tovar, Hugo Ricardo Domínguez-Malagón

{"title":"Ultrastructural differences between synovial sarcoma and solitary fibrous tumor: comparative study in adult patients from the National Cancer Institute of Mexico.","authors":"Guillermo Ernesto Corredor-Alonso, Leonardo Saúl Lino-Silva, Erika Yazmin López-Flores, Tatiana Velásquez-Tovar, Hugo Ricardo Domínguez-Malagón","doi":"10.1080/01913123.2024.2313742","DOIUrl":"10.1080/01913123.2024.2313742","url":null,"abstract":"Synovial sarcoma (SS) and solitary fibrous tumor (SFT) are entities with considerable morphological and immunohistochemical similarities that sometimes show a non-confirmatory profile (TLE1 negative, CD34 and focal or negative STAT6 and lack of specific fusion IHC markers), in which the utility ultrastructure is unknown. A cross-sectional, retrospective, analytical, nonexperimental study was carried out by the Department of Pathology of the National Cancer Institute of Mexico (INCan) e from January 1, 2009 to December 31, 2018. With 17 SFT cases with diffuse or focal CD34 and STAT6 positivity and 18 cases of SS with positive FISH molecular test t(X:18) breakapart were studied by electron microscopy of fresh glutaraldehyde fixed or paraffin-embedded tissue. The ultrastructural findings with a significant difference present in the SS were tandem tight junctions, desmosomes and abundance of dilated rough endoplasmic reticulum (RER) cisternae (p < 0.001, 0.003, and 0.001, respectively); while in the (SFT) the presence of abundant glycogen, basal lamina, long and slender cytoplasmic processes, pinocytic vesicles, hemidesmosomes, and/or dense plaques, collagen skein, and microvilli-like buds (p = 0.028, 0.005, and <0.001 for the last five). We then infer that the five distinctive markers of the SFT are the collagen skeins intermingled with cellular processes in a shape of \"squid can,\" and the pinocytic vesicles as they were not observed in any case of SS. Conversely, tandem junctions were not found in any SFT case. Although the presence of multivesicular buds in the SFT was not significant, it had not been previously described.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"213-220"},"PeriodicalIF":1.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139724194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robert Erlandson obituary (September 4, 1937-February 4, 2024): a true giant and great champion of ultrastructural pathology. 罗伯特-厄兰森（Robert Erlandson）（1937 年 9 月 4 日-2024 年 2 月 4 日）：超微结构病理学的真正巨人和伟大倡导者。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2024-05-03 Epub Date: 2024-03-31 DOI: 10.1080/01913123.2024.2334996

David M Parham

引用次数: 0

Effect of platelet rich plasma versus melatonin on testicular injury induced by Busulfan in adult albino rats: a histological and immunohistochemical study. 富血小板血浆和褪黑素对成年白化大鼠布舒凡所致睾丸损伤的影响：组织学和免疫组化研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2024-05-03 Epub Date: 2024-02-29 DOI: 10.1080/01913123.2024.2322567

Eman M Askar, Amira M Abdelmegid, Laila M Elshal, Mohamed A Shaheen

{"title":"Effect of platelet rich plasma versus melatonin on testicular injury induced by Busulfan in adult albino rats: a histological and immunohistochemical study.","authors":"Eman M Askar, Amira M Abdelmegid, Laila M Elshal, Mohamed A Shaheen","doi":"10.1080/01913123.2024.2322567","DOIUrl":"10.1080/01913123.2024.2322567","url":null,"abstract":"This study was done to estimate the testicular histological alterations induced by Busulfan (BUS) and compare the possible protective effects of melatonin (MT) and platelet rich plasma (PRP) in a rat model. Sixty-four male rats were dispersed into: control group, BUS group, melatonin group, and PRP group. Blood samples were processed for biochemical analysis. Tissue specimens were managed for light and electron microscopic studies. Immunohistochemical expression of vimentin and proliferating cell nuclear antigen (PCNA) was performed. Busulfan induced severe testicular damage in all studied methodologies. It showed a statistically significant decrease in serum testosterone and elevation of MDA when compared to the control group. Abnormal testicular cytostructures suggesting defective spermatogenesis were observed: distorted seminiferous tubules, deformed spermatogenic cells, low germinal epithelium height, few mature spermatozoa, and also deformed barrier. Vimentin and PCNA expressions were reduced. Ultrastructurally, Sertoli cells and the blood testis barrier were deformed, spermatogenic cells were affected, and mature spermatozoa were few and showed abnormal structure. Both melatonin and PRP induced improvement in all the previous parameters and restoration of spermatogenesis as confirmed by improvement of Johnsen's score from 2.6 ± .74 to 7.6 ± .92. In conclusion, melatonin and PRP have equal potential to ameliorate the testicular toxicity of BUS. Melatonin can provide a better noninvasive way to combat BUS induced testicular injury.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"192-212"},"PeriodicalIF":1.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139991286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0