Ultrastructural Pathology最新文献_第3页

Malignant glomus tumor: a rare case with an ultrastructural study. 恶性血管球瘤：罕见病例的超微结构研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-30 DOI: 10.1080/01913123.2025.2499140

Corredor-Alonso Guillermo Ernesto, Arredondo-Ruiz Pedro, Martinez-Tlahuel Jorge Luis, Dominguez-Malagon Hugo Ricardo

引用次数: 0

Which has a more protective effect against neuronal changes in the cingulate cortex of newborn rats of diabetic mothers: omega 3 or glutathione? 对于糖尿病母亲的新生大鼠，欧米茄3和谷胱甘肽哪一种对其扣带皮层神经元变化的保护作用更强？

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-11 DOI: 10.1080/01913123.2025.2490161

Esraa Nafadi, Walaa Farag

{"title":"Which has a more protective effect against neuronal changes in the cingulate cortex of newborn rats of diabetic mothers: omega 3 or glutathione?","authors":"Esraa Nafadi, Walaa Farag","doi":"10.1080/01913123.2025.2490161","DOIUrl":"https://doi.org/10.1080/01913123.2025.2490161","url":null,"abstract":"Different researches suggests that unchecked maternal hyperglycemia during pregnancy may negatively impact the offspring's neurodevelopment. Omega 3 polyunsaturated fatty acids are vital antioxidant micronutrients for neurological health. Glutathione (GSH) is an essential non-enzymatic antioxidant in mammalian cells. The study aimed to assess the possible ameliorative effect of omega3 versus GSH against the neuronal changes in the cingulate cortex of new born rats of diabetic mothers. Fifty female rats were divided randomly into 5 equal groups: Group I given saline, group II where induction of diabetes by single intraperitoneal injection of 150 mg/kg of alloxan monohydrate, group III given oral omega 3 at a dose of 300 mg/kg, group IV given intraperitoneal injections of GSH at a dose of (200 mg/kg) and group V given both omega 3 and GSH with the same routes and regimens as group III and IV throughout the whole pregnancy. The newborn of each group was collected and anaesthetized then brain specimens were extracted and processed for the light, immunohistochemical and electron microscopic studies. Group II showed marked degenerative changes in the all layers of cingulate cortex by light and electron microscopy. The cingulate cortex of group V showed the most degree of improvement in comparison to group III and IV. There was strong caspase3 immunoreaction in group II while weak reaction appeared with group III, IV and V. We concluded that the combined administration of omega 3 and GSH mitigate the adverse effects of maternal diabetes on the newborn's cingulate cortex.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"243-256"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent trends in the causation of peritoneal mesothelioma: fiber burden analysis of ten cases. 腹膜间皮瘤病因的最新趋势：10例纤维负荷分析。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-03-26 DOI: 10.1080/01913123.2025.2483226

Victor L Roggli, Stasha Novakovic, Andrew J Ghio, Huihua Li, Sergio Pina-Oviedo, John M Carney, Thomas A Sporn, Carolyn H Glass, Elizabeth N Pavlisko

{"title":"Recent trends in the causation of peritoneal mesothelioma: fiber burden analysis of ten cases.","authors":"Victor L Roggli, Stasha Novakovic, Andrew J Ghio, Huihua Li, Sergio Pina-Oviedo, John M Carney, Thomas A Sporn, Carolyn H Glass, Elizabeth N Pavlisko","doi":"10.1080/01913123.2025.2483226","DOIUrl":"10.1080/01913123.2025.2483226","url":null,"abstract":"Mesothelioma in the past has been strongly associated with a history of asbestos exposure. Studies have shown that, on average, a higher dose of asbestos exposure is required for the development of peritoneal mesothelioma, and a smaller percentage of cases are asbestos related. Non-asbestos-related causes have been reported, including prior therapeutic radiation, genetic predisposition, and chronic inflammation (e.g. Crohn disease, endometriosis, ventriculo-peritoneal shunts, and diverticulitis). Cases in children have also been reported. Recent studies have shown a decreasing trend in fiber burdens and percentage of asbestos-related mesotheliomas, with similar observations in epidemiological studies. We performed fiber burden analysis on lung tissue in 10 cases (six men, four women) of peritoneal mesothelioma since 2010. Fiber analysis was performed using the sodium hypochlorite digestion technique, with asbestos body concentrations determined by light microscopy. Fiber concentrations and types were determined by scanning electron microscopy. The median age for the six men was 62 years (range: 53-75 years). Three cases were epithelioid type and three were biphasic. Two of six cases (33%) had an elevated lung fiber burden, with one case exclusively crocidolite and the other predominately amosite. The median age for the four women was 55 years (range: 39-63 years). Two cases were epithelioid type and two were biphasic. None of the four had an elevated lung fiber burden. Our findings are consistent with contemporary epidemiological studies indicating that a minority of peritoneal mesotheliomas occurring in men are asbestos related and very few are asbestos related in women.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"288-295"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient isolation and characterization of Serum-Derived Exosomes: evaluating ultracentrifugation and Total Exosome Isolation Reagent based precipitation. 血清衍生外泌体的高效分离和表征：评估超离心和基于沉淀的总外泌体分离试剂。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-25 DOI: 10.1080/01913123.2025.2507698

Ranjana Bharti, Munish Kumar, Veena Devi, Asha Rao, Ashish Aggarwal, Tulika Gupta

{"title":"Efficient isolation and characterization of Serum-Derived Exosomes: evaluating ultracentrifugation and Total Exosome Isolation Reagent based precipitation.","authors":"Ranjana Bharti, Munish Kumar, Veena Devi, Asha Rao, Ashish Aggarwal, Tulika Gupta","doi":"10.1080/01913123.2025.2507698","DOIUrl":"10.1080/01913123.2025.2507698","url":null,"abstract":"Exosomes are extracellular vesicles that carry biomolecular cargos such as proteins, lipids, RNA, and DNA. These molecules play crucial roles in cell-to-cell communication and are involved in various physiological and pathological processes. Due to their potential as biomarkers for disease diagnosis and prognosis, research has increasingly focused on developing more efficient methods for their isolation and characterization. In this study, blood samples were collected from 30 participants, and serum was subsequently isolated. Serum-derived exosomes (SDEs) were extracted using ultracentrifugation (UC) as well as the Total Exosome Isolation Reagent. Modifications to the UC method were implemented to improve yield and purity, and a detailed description of the method is also provided. The exosomes were characterized by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), and Western Blotting (WB) to evaluate their size, morphology, and protein content. The exosome yields from both isolation methods were evaluated using the BCA assay. Protein estimation suggested that the Total Exosome Isolation Reagent produced exosome concentrations that were 10-fold higher compared to those obtained through ultracentrifugation. Morphological analysis showed that exosomes exhibited circular, spherical, and irregular shapes, with diameters ranging from 30 to 200 nm. Western Blotting confirmed the presence of exosomal markers (TSG101, ALIX, LAMP2, and CD63) in the SDEs. In conclusion, both ultracentrifugation and the Total Exosome Isolation Reagent effectively isolate SDEs. Thus, although both methods are viable, modified ultracentrifugation is the preferred choice for applications due to its cost-effectiveness and suitability for achieving pure protein yields.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"340-353"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A primary renal neuroendocrine tumor presenting in a fifty-five-year-old woman: a report of a rare entity. 原发性肾神经内分泌肿瘤呈现在一个55岁的妇女：一个罕见的实体报告。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-06-18 DOI: 10.1080/01913123.2025.2520217

Rodney E Shackelford, Ardenne Martin, Charu Shastri, Muhammad Tahir, Guillermo A Herrera, Christopher E Keel, Carlina Medelaire

引用次数: 0

Correction. 修正。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-21 DOI: 10.1080/01913123.2025.2508065

引用次数: 0

Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study. 杨梅素改善过氧化氢诱导的人间充质干细胞氧化应激的影响：一项超微结构和免疫细胞化学研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-17 DOI: 10.1080/01913123.2025.2494618

Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta

{"title":"Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study.","authors":"Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta","doi":"10.1080/01913123.2025.2494618","DOIUrl":"https://doi.org/10.1080/01913123.2025.2494618","url":null,"abstract":"The development of new strategies to raise the survival and viability of transplanted mesenchymal stem cells (MSCs) is very important for the therapeutic potential of stem cells. The natural flavonoid myricetin has anticancer, antioxidant, anti-inflammatory and antiapoptotic effects. The effects of myricetin on human umbilical cord-derived MSCs (HUC-MSCs) induced oxidative stress with hydrogen peroxide (H2O2) were evaluated by transmission electron microscopy (TEM) and immunocytochemistry (ICC) staining. Myricetin showed an increase in the number of live cells, a decrease in caspase-3 and tumor necrosis factor-α (TNF-α) ICC staining intensity, an increase in the translocase of the mitochondrial inner membrane 17 (TIM17) ICC staining intensity, and a decrease in degeneration of cell ultrastructure in TEM against oxidative stress damage in HUC-MSCs. The results suggest that myricetin prevents oxidative stress-induced apoptosis and inflammation in the HUC-MSCs. Myricetin can be combined with HUC-MSCs in cell culture and considered as a supportive alternative treatment option.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"257-264"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of olanzapine on testes of adult albino rats and the possible role of granulocyte colony stimulating factor versus umbelliferone light and electron microscopic study. 奥氮平对成年白化病大鼠睾丸的影响及粒细胞集落刺激因子对伞蛋白的作用。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-24 DOI: 10.1080/01913123.2025.2495159

Maha A Khattab, Samah M Ahmed, Haidy G Salama, Noura H Mekawy

{"title":"Effect of olanzapine on testes of adult albino rats and the possible role of granulocyte colony stimulating factor versus umbelliferone light and electron microscopic study.","authors":"Maha A Khattab, Samah M Ahmed, Haidy G Salama, Noura H Mekawy","doi":"10.1080/01913123.2025.2495159","DOIUrl":"10.1080/01913123.2025.2495159","url":null,"abstract":"Olanzapine (OLZ) is one of atypical antipsychotic drugs (second generation) used for treating schizophrenia, manic, and mixed episodes of bipolar disorder. Continuous evaluation of its effects is necessary, and there is a need to explore alternative natural products as G-CSF and UMB to manage potential side effects. This research designed to mitigate the atypical antipsychotic drugs' adverse effects through biochemical analyses, light and electron microscopic studies. Fifty-six rats were divided into five groups: Control, OLZ, G-CSF, UMB, and Recovery groups. End body and testicular weights, serum testosterone, testicular MDA levels, and seminal analysis were recorded. Testicular specimens were processed to evaluate histological structure, PCNA, and CD34 immune expression. Morphometric and statistical analyses were also performed. OLZ group exhibited a distorted testicular structure, a significant increase in end body and a decline in testicular weight, a significant decline in the serum level of testosterone level, testicular MDA, and seminal analysis parameters. Furthermore, disturbed histoarchitecture, reduction in PCNA, and elevation in CD34 immunoreaction were observed. These alterations were partially attenuated by G-CSF therapy, whereas UMB significantly improved all parameters. In conclusion, UMB, and to a lesser degree G-CSF, appeared to be superior therapeutic options by attenuating oxidative stress and restoring intact histological structure and biochemical parameters.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"265-287"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic lymphocytic leukemia with associated renal invasion and light chain-restricted deposition membranous nephropathy: a case report and literature review. 慢性淋巴细胞白血病合并肾侵犯和轻链限制性沉积膜性肾病1例报告并文献复习。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-25 DOI: 10.1080/01913123.2025.2506742

Chong Geng, Hongmin Luo

{"title":"Chronic lymphocytic leukemia with associated renal invasion and light chain-restricted deposition membranous nephropathy: a case report and literature review.","authors":"Chong Geng, Hongmin Luo","doi":"10.1080/01913123.2025.2506742","DOIUrl":"10.1080/01913123.2025.2506742","url":null,"abstract":"Chronic lymphocytic leukemia (CLL) is a prevalent hematological malignancy that significantly affects the kidneys as an extramedullary organ. Reports from autopsy studies have shown the infiltration of CLL cells into the renal parenchymal in 63-93% of cases. Glomerular diseases associated with CLL are relatively rare, occurring in approximately 2% of patients and often presenting as nephrotic syndrome. The most common histological pattern observed in CLL-associated glomerular diseases is membranoproliferative glomerulonephritis, followed by minimal change disease and membranous nephropathy. In this report, we presented a case of a 69-year-old male patient with CLL who developed nephrotic syndrome. The diagnosis of CLL was confirmed through bone marrow and renal biopsies, which revealed the presence of CLL tumor cells in the renal interstitium along with membranous nephropathy characterized by light chain-restricted deposits. The tumor cells present in the renal interstitium and glomeruli of the patient expressed identical light chain restrictions, which suggested that the membranous nephropathy was secondary and possibly induced by the deposition of tumor-associated antigens. Treatment with a combination of fludarabine, cyclophosphamide, and rituximab led to the remission of both the CLL and nephrotic syndrome, with no recurrence observed during the follow-up period.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"377-383"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary ciliary dyskinesia cases bronchoscopic sampling and TEM analysis: sampling & diagnosis in PCD. 原发性纤毛运动障碍病例的支气管镜取样与透射电镜分析：PCD的取样与诊断。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-30 DOI: 10.1080/01913123.2025.2498418

Deniz Doğan Mülazimoğlu, Oya Evirgen, Oya Kayacan, Demet Karnak

{"title":"Primary ciliary dyskinesia cases bronchoscopic sampling and TEM analysis: sampling & diagnosis in PCD.","authors":"Deniz Doğan Mülazimoğlu, Oya Evirgen, Oya Kayacan, Demet Karnak","doi":"10.1080/01913123.2025.2498418","DOIUrl":"10.1080/01913123.2025.2498418","url":null,"abstract":"Primary ciliary dyskinesia (PCD) is a rare, genetically heterogeneous disorder characterized by impaired ciliary structure and function, leading to chronic respiratory symptoms and recurrent infections. Despite its clinical significance, PCD diagnosis remains challenging due to its variable presentation and the lack of a gold standard diagnostic test. Specific clinical criteria, including neonatal respiratory distress and laterality defects, aid in suspicion of PCD, but confirmatory diagnosis often requires a combination of tests. In this study, we aimed to assess the efficacy of bronchoscopic techniques in obtaining respiratory epithelial samples for transmission electron microscopy (TEM) analysis. We enrolled adults with bronchiectasis and suspected PCD who underwent fiberoptic bronchoscopy. Bronchial forceps and brush biopsies were obtained from specific bronchial segments under conscious sedation. Tissue samples were processed for TEM analysis to identify ultrastructural axonemal defects associated with PCD. Our study included 10 patients (3 females, 7 males) aged 19-38 years, with detailed demographics and clinical characteristics provided. Evaluation of tracheobronchial biopsy samples revealed higher histological scores for the presence of ciliated cells and transverse sections of cilia in pellets obtained from brush biopsies and fixative solutions of forceps biopsy compared to forceps biopsy tissue samples. Electron microscopic examination of ultra-thin sections demonstrated abundant ciliated cells and abnormal cilia structures, aiding in the diagnosis of PCD in pellets. PCD represents a significant etiology of bronchiectasis, emphasizing the need for accurate diagnosis and appropriate management strategies. Our findings highlight the importance of bronchoscopic techniques, including bronchial brushing alongside forceps biopsies, in enhancing diagnostic yield and guiding timely intervention to improve patient outcomes.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"371-376"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0