Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta
{"title":"杨梅素改善过氧化氢诱导的人间充质干细胞氧化应激的影响：一项超微结构和免疫细胞化学研究。","authors":"Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta","doi":"10.1080/01913123.2025.2494618","DOIUrl":null,"url":null,"abstract":"<p><p>The development of new strategies to raise the survival and viability of transplanted mesenchymal stem cells (MSCs) is very important for the therapeutic potential of stem cells. The natural flavonoid myricetin has anticancer, antioxidant, anti-inflammatory and antiapoptotic effects. The effects of myricetin on human umbilical cord-derived MSCs (HUC-MSCs) induced oxidative stress with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) were evaluated by transmission electron microscopy (TEM) and immunocytochemistry (ICC) staining. Myricetin showed an increase in the number of live cells, a decrease in caspase-3 and tumor necrosis factor-α (TNF-α) ICC staining intensity, an increase in the translocase of the mitochondrial inner membrane 17 (TIM17) ICC staining intensity, and a decrease in degeneration of cell ultrastructure in TEM against oxidative stress damage in HUC-MSCs. The results suggest that myricetin prevents oxidative stress-induced apoptosis and inflammation in the HUC-MSCs. Myricetin can be combined with HUC-MSCs in cell culture and considered as a supportive alternative treatment option.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"257-264"},"PeriodicalIF":1.1000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study.\",\"authors\":\"Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta\",\"doi\":\"10.1080/01913123.2025.2494618\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The development of new strategies to raise the survival and viability of transplanted mesenchymal stem cells (MSCs) is very important for the therapeutic potential of stem cells. The natural flavonoid myricetin has anticancer, antioxidant, anti-inflammatory and antiapoptotic effects. The effects of myricetin on human umbilical cord-derived MSCs (HUC-MSCs) induced oxidative stress with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) were evaluated by transmission electron microscopy (TEM) and immunocytochemistry (ICC) staining. Myricetin showed an increase in the number of live cells, a decrease in caspase-3 and tumor necrosis factor-α (TNF-α) ICC staining intensity, an increase in the translocase of the mitochondrial inner membrane 17 (TIM17) ICC staining intensity, and a decrease in degeneration of cell ultrastructure in TEM against oxidative stress damage in HUC-MSCs. The results suggest that myricetin prevents oxidative stress-induced apoptosis and inflammation in the HUC-MSCs. Myricetin can be combined with HUC-MSCs in cell culture and considered as a supportive alternative treatment option.</p>\",\"PeriodicalId\":23430,\"journal\":{\"name\":\"Ultrastructural Pathology\",\"volume\":\"49 3\",\"pages\":\"257-264\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ultrastructural Pathology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1080/01913123.2025.2494618\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MICROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrastructural Pathology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/01913123.2025.2494618","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/17 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MICROSCOPY","Score":null,"Total":0}
Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study.
The development of new strategies to raise the survival and viability of transplanted mesenchymal stem cells (MSCs) is very important for the therapeutic potential of stem cells. The natural flavonoid myricetin has anticancer, antioxidant, anti-inflammatory and antiapoptotic effects. The effects of myricetin on human umbilical cord-derived MSCs (HUC-MSCs) induced oxidative stress with hydrogen peroxide (H2O2) were evaluated by transmission electron microscopy (TEM) and immunocytochemistry (ICC) staining. Myricetin showed an increase in the number of live cells, a decrease in caspase-3 and tumor necrosis factor-α (TNF-α) ICC staining intensity, an increase in the translocase of the mitochondrial inner membrane 17 (TIM17) ICC staining intensity, and a decrease in degeneration of cell ultrastructure in TEM against oxidative stress damage in HUC-MSCs. The results suggest that myricetin prevents oxidative stress-induced apoptosis and inflammation in the HUC-MSCs. Myricetin can be combined with HUC-MSCs in cell culture and considered as a supportive alternative treatment option.
期刊介绍：
Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology.
Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics:
Advances in the uses of electron microscopic and immunohistochemical techniques
Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis
Important new, investigative, clinical, and diagnostic EM methods.
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