Ultrastructural Pathology最新文献_第4页

Effects of metformin on CCl4-induced pulmonary fibrosis via autophagy pathway in rats: a histological, electron microscopic, and immunohistochemical study. 二甲双胍通过自噬途径对ccl4诱导的大鼠肺纤维化的影响：组织学、电镜和免疫组织化学研究

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-04-19 DOI: 10.1080/01913123.2026.2650192

Fatma Y Meligy, Amal T Abo Elghait, Nessren M Abd El-Rady, Amira A Kamel, Fatma K Gameaa

{"title":"Effects of metformin on CCl4-induced pulmonary fibrosis via autophagy pathway in rats: a histological, electron microscopic, and immunohistochemical study.","authors":"Fatma Y Meligy, Amal T Abo Elghait, Nessren M Abd El-Rady, Amira A Kamel, Fatma K Gameaa","doi":"10.1080/01913123.2026.2650192","DOIUrl":"10.1080/01913123.2026.2650192","url":null,"abstract":"Lung fibrosis is defined as fibrotic change of the lung architecture within 2.5-3 years of diagnosis, ultimately leading to respiratory failure and death. Prolonged exposure to high concentrations of carbon tetrachloride, especially vapor, can cause lung destruction. Autophagy was crucial for maintaining the organs' homeostasis. Deficit in autophagy has been commonly associated with fibrosis and pneumonia. Recent research suggests that metformin may provide protection against a variety of lung ailments. To investigate how metformin protects against lung damage caused by carbon tetrachloride via the autophagy pathway, 30 adult male albino rats (10 rats each) were divided into three equal groups. One group served as the control. Group 2: received CCl4 1.5 mg/kg twice weekly S.C. for a duration of 12 weeks. Group 3: Oral metformin and CCl4 were administered for 12 weeks at a dose of 100 mg/kg/day and 1.5 mg/kg twice weekly S.C. After the animal died, its lungs were taken out of each group and studied under an electron and light microscope in addition to being used for immunological research. The histological abnormalities in the lung tissue caused by the administration of CCl4 included areas of tissue destruction, congestion, and fibrosis. Pneumocyte type I, type II, and collagen fiber deposition were found to have changed ultrastructurally. Concurrent metformin and CCl4 treatment resulted in improvements. The histological and biochemical effects of CCl4 on lung tissue can be modulated by administration of metformin.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"159-175"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural neuroprotection by intrathecal interleukin-6 antagonism in a rat model of permanent focal cerebral ischemia. 鞘内白介素-6拮抗剂对永久性局灶性脑缺血大鼠模型的超微结构神经保护作用。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-08-11 DOI: 10.1080/01913123.2026.2714959

Mustafa Zeren, Faruk İldan

{"title":"Ultrastructural neuroprotection by intrathecal interleukin-6 antagonism in a rat model of permanent focal cerebral ischemia.","authors":"Mustafa Zeren, Faruk İldan","doi":"10.1080/01913123.2026.2714959","DOIUrl":"10.1080/01913123.2026.2714959","url":null,"abstract":"This study aimed to determine whether intrathecal administration of an interleukin-6 (IL-6) neutralizing antibody could reduce ultrastructural neuronal and vascular damage in a rat model of permanent middle cerebral artery occlusion (MCAO). Forty male Wistar rats were randomly assigned to four groups: Control, Sham-operated, Occlusion (MCAO + saline), and Treatment (MCAO + anti-rat IL-6 antibody). One week later, ischemic core brain tissue was processed for transmission electron microscopy to evaluate neuronal, axonal, and microvascular integrity. The Occlusion group showed severe ischemic injury, including mitochondrial swelling with cristolysis, cytoplasmic vacuolization, axonal edema, endothelial swelling, and perivascular astrocyte edema. By contrast, the Treatment group demonstrated marked ultrastructural preservation. Endothelial swelling and perivascular edema were reduced, neuronal nuclei were more preserved, and myelin sheath separation in white matter fibers was less pronounced than in the Occlusion group. Direct intrathecal IL-6 blockade was associated with qualitative cellular-level neuroprotection after permanent focal cerebral ischemia. The treatment attenuated inflammatory vascular injury and white matter damage, supporting IL-6 as a potential therapeutic target for limiting secondary stroke injury.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"452-461"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148713825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural characterization of extracellular vesicles produced by Encephalitozoon cuniculi-infected B-1 cells. cucuuliencephalitozoon感染B-1细胞产生的细胞外囊泡的超微结构特征。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2025-10-28 DOI: 10.1080/01913123.2025.2580509

Alicia Herrera Gutiérrez, Rayane Cristina Bego, Diva Denelle Spadacci-Morena, Patrícia Batista Xander, Anuska Marcelino Alvares-Saraiva, Elizabeth Cristina Pérez, Uziel Castillo Velázquez, Maria Anete Lallo

引用次数: 0

Transmembrane mucin 1 is a polarity protein in the organization of apical surface microplicae in oral epithelium. 跨膜粘蛋白1是口腔上皮顶端表面微皱组织中的一种极性蛋白。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2025-10-27 DOI: 10.1080/01913123.2025.2578223

Lauriina Soiniemi, Matias Kanniainen, Ella Suomela, Saga Ramsay, Heli Jäsberg, Bina Kashyap, Arja M Kullaa

{"title":"Transmembrane mucin 1 is a polarity protein in the organization of apical surface microplicae in oral epithelium.","authors":"Lauriina Soiniemi, Matias Kanniainen, Ella Suomela, Saga Ramsay, Heli Jäsberg, Bina Kashyap, Arja M Kullaa","doi":"10.1080/01913123.2025.2578223","DOIUrl":"10.1080/01913123.2025.2578223","url":null,"abstract":"This study aims to provide a new insight into the oral mucosal defense, and to investigate the oral microbial attachment to the oral mucosa. A total of 68 oral mucosal samples were prepared for light microscopy, immunohistochemistry (IHC), and both scanning and transmission electron microscopy. Tissue samples were IHC stained for transmembrane mucin 1 (tMUC1), which was also examined with immuno-scanning electron microscopy (iSEM). The scanning electron microscopy (SEM) images were procured to analyze the attachment of microbes on different parts of the oral mucosa. The IHC for tMUC1 presented its localization in the apical portion of the superficial surface of non-keratinized epithelial cells, whereas no such expression was observed in keratinized epithelium. In SEM images, the microplicae (MPL) structure organized in non-keratinized epithelial cells, and iSEM showed tMUC1 localized at the tips of MPLs. Microbial attachment was low in the areas showing tMUC1-MPL-structures, whereas more abundant in the areas that lack tMUC1 attachment with MPL. The superficial MPL and the attachment of tMUC1 form a complex that functionally prevents the attachment of microbes. The iSEM confirms that tMUC1 is a polarity protein, which generates the apical MPL structure in oral epithelial surface cells and together provides protection against microbial colonization and invasion.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"1-10"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145378896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of deltamethrin on the testicular structure of adult male albino rats and the possible protective role of lutein: a histological, immunohistochemical, and ultrastructural study. 溴氰菊酯对成年雄性白化大鼠睾丸结构的影响及叶黄素可能的保护作用：组织学、免疫组织化学和超微结构研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-05-08 DOI: 10.1080/01913123.2026.2669306

Eman E Elwakeel, Nehal Fahmy Shaheen

{"title":"The impact of deltamethrin on the testicular structure of adult male albino rats and the possible protective role of lutein: a histological, immunohistochemical, and ultrastructural study.","authors":"Eman E Elwakeel, Nehal Fahmy Shaheen","doi":"10.1080/01913123.2026.2669306","DOIUrl":"10.1080/01913123.2026.2669306","url":null,"abstract":"Lutein provides a promising approach to protect the testes against damage induced by deltamethrin. The current study was applied to investigate the protective effect of lutein on the testis of adult male albino rats against testicular toxicity induced by deltamethrin. Sixty adult male albino rats were randomly allocated into six experimental groups. Group I served as control, group II received lutein (40 mg/kg/day orally), group III received deltamethrin (5 mg/kg/day orally), group IV received deltamethrin (5 mg/kg/day orally) concomitant with lutein (40 mg/kg/day orally), all for 4 weeks. Group V received lutein (40 mg/kg/day) throughout 8 weeks, whereas deltamethrin was given at week 5 till the end of week 8. Group VI served as a withdrawal group in which animals received deltamethrin for four weeks, followed by a four-week treatment-free period to assess the reversibility of its toxic effects. Biochemical, histological, and ultrastructural techniques were used to evaluate the testicular changes. Deltamethrin-treated rats showed decreased serum testosterone. Testicular tissue exhibited pronounced histopathological changes. Histopathological examination of testicular tissue showed that apoptosis was observed in the basal germ cells, primary and secondary spermatocytes. The testes exhibited distorted Sertoli cells accompanied by depletion of spermatogenic cells, along with significant reductions in seminiferous tubular diameter and serum testosterone levels relative to control. While the percentage of apoptotic cells was significantly increased. Group V showed improvement in histological structure, the testosterone levels, and other morphometrical parameters. Pretreatment of lutein to deltamethrin-treated rats prevents the histopathological alterations by deltamethrin in testis.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"268-282"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal stem cell-derived microvesicles versus sodium glucose cotransporter-2 inhibitors in ameliorating acute renal cortical injury of ischemia reperfusion rat model. 间充质干细胞来源的微泡与葡萄糖共转运蛋白-2抑制剂钠改善缺血再灌注大鼠急性肾皮质损伤模型。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-08-06 DOI: 10.1080/01913123.2026.2713009

Amira M Abdelmegid, Mai E Abdelhady, Mohamed Abdelmaabod Abdel Hamid, Manar M Ali

{"title":"Mesenchymal stem cell-derived microvesicles versus sodium glucose cotransporter-2 inhibitors in ameliorating acute renal cortical injury of ischemia reperfusion rat model.","authors":"Amira M Abdelmegid, Mai E Abdelhady, Mohamed Abdelmaabod Abdel Hamid, Manar M Ali","doi":"10.1080/01913123.2026.2713009","DOIUrl":"10.1080/01913123.2026.2713009","url":null,"abstract":"Acute kidney injury (AKI) is primarily caused by renal ischemia/reperfusion (I/R) injury, which is clinically linked to significant morbidity and death in both developed and developing countries. Therefore, the aim of this work is to compare the possible ameliorative effect of dapagliflozin (DAPA) versus human umbilical cord mesenchymal stem cells derived microvesicles (HUC-MSC-MVs) through biochemical, light, and electron microscope studies. Fifty-six adult male albino rats were divided into four groups: control, I/R, I/R+DAPA, and I/R+MVs groups. Both serum and tissue samples were collected for biochemical analysis. Renal specimens were processed to evaluate the histological structure, collagen content, desmin, and β-catenin immune expression. Morphometric and statistical analyses were performed. I/R group revealed prominent histoarchitectural alterations and a significant rise in the serum urea, creatinine, oxidative stress markers, and inflammatory mediators. Alterations were partially attenuated by DAPA therapy, whereas HUC-MSC-MVs significantly improved all parameters. We concluded that MSC-MVs administration exert a more significant ameliorative effect comparing to DAPA therapy via attenuating the oxidative stress and inflammation in addition to, restoring angiogenesis and cell proliferation. However, these findings remain preclinical and further researches are acclaimed for dose optimization, safety evaluation, and clinical validation before application in human AKI.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"431-451"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148685952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gabapentin protects against streptozotocin-induced diabetic retinopathy in rats: histological, immunohistochemical and ultrastructural study. 加巴喷丁对链脲佐菌素致大鼠糖尿病视网膜病变的保护作用：组织学、免疫组织化学和超微结构研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-09-17 DOI: 10.1080/01913123.2026.2729366

Asmaa M Alhoseiny, Shereen S El-Habashy, Shireen A Mazroa, Nahla Reda Sarhan

{"title":"Gabapentin protects against streptozotocin-induced diabetic retinopathy in rats: histological, immunohistochemical and ultrastructural study.","authors":"Asmaa M Alhoseiny, Shereen S El-Habashy, Shireen A Mazroa, Nahla Reda Sarhan","doi":"10.1080/01913123.2026.2729366","DOIUrl":"10.1080/01913123.2026.2729366","url":null,"abstract":"Diabetic retinopathy (DR) is a main cause of visual impairment globally. DR is mostly considered neurovascular degeneration. Gabapentin exerts antioxidant and anti-inflammatory effects. This study aimed to explore gabapentin's neuroprotective potential against DR. Forty-five adult male rats were divided into 3 groups; Control group I. Diabetic group II received an intraperitoneal dose of streptozotocin (STZ) (50 mg/kg) to induce diabetes. Blood glucose was assessed 48 h following STZ. Rats with blood glucose ≥ 250 mg/dl were considered diabetic. Gabapentin-treated diabetic group III in which the diabetic rats received gabapentin (300 mg/kg/12 h) by gastric tube. After 8 weeks, retinal samples were prepared for histological, immunohistochemical and ultrastructural studies. Diabetic group II revealed diminished cells of outer and inner nuclear layers, reduction in retinal thickness and ganglion cell number; and elevation of caspase-3 and Glial fibrillary acidic protein (GFAP) immune-expression and number of blood vessels expressing vascular endothelial growth factor. Disorganized discs of photoreceptors and neurons with shrunken nuclei were found. Gabapentin-treated diabetic group III showed preserved histology and ultrastructure and reduction in caspase-3 and GFAP immune-expression and number of blood vessels. In conclusion, gabapentin has neuroprotective potential against DR and might represent a valuable therapeutic tool in the future.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"474-498"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of permethrin on the ultrastructural morphology of rat kidney: electron microscopic study. 氯菊酯对大鼠肾脏超微结构形态影响的电镜研究。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2025-12-03 DOI: 10.1080/01913123.2025.2596206

Tuğba Kotil, Nazan Deniz Yön

{"title":"The effects of permethrin on the ultrastructural morphology of rat kidney: electron microscopic study.","authors":"Tuğba Kotil, Nazan Deniz Yön","doi":"10.1080/01913123.2025.2596206","DOIUrl":"10.1080/01913123.2025.2596206","url":null,"abstract":"A pesticide, Permethrin, that has low toxicity and widespread usage, is the most frequently detected in house dust. This study aimed to investigate the effects of low-dose permethrin exposure on the ultrastructural nephrotoxicity of rat kidneys at the microscopic level. 28 Wistar albino rats were randomly assigned to four groups. Doses of 20 mg, 40 mg, and 75 mg/kg permethrin were administered to the experimental groups via gavage for 2 weeks. The control group received corn oil. After the experiment, kidneys collected from the sacrificed animals were fixed with 2.5% glutaraldehyde and embedded in epon. Semi-thin sections were evaluated via light microscope, and thin sections were examined with a transmission electron microscope. Semi-thin sections showed brush border loss in experimental groups, and also the cellular arrangement of the tubules was altered. Thin sections of the control group, proximal tubules, and podocyte cells of glomerulus were intact and showed normal morphology. Cytoplasmic vacuolization, degenerated mitochondria, and lysosome accumulation were observed in tubule cells in experimental groups, increasing in a dose-dependent manner. Autophagic vacuoles were present in the 40 and 75 mg groups. Necrotic cells were present in the 75 mg group. Marked endoplasmic reticulum dilatation and ribosome loss were observed in the podocytes of glomerulus of the experimental groups at higher doses. The highest group showed necrotic cells, and the glomerular basement membrane structure was disrupted. Our findings suggest that low-dose, short-term permethrin exposure has a toxic effect and negatively affects renal ultrastructure.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"92-103"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145662101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of concurrent administration of granulocyte colony stimulating factor and pirfenidone on experimentally induced lung fibrosis in adult male albino rats (a histological, immunohistochemical & ultrastructural study). 同时给予粒细胞集落刺激因子和吡非尼酮对成年雄性白化大鼠实验性肺纤维化的影响（组织学、免疫组织化学和超微结构研究）。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-05-25 DOI: 10.1080/01913123.2026.2668409

Samar R Mohamed, Sally A Selim, Sanaa A Mohamed, Karima F Abdelfadeel

{"title":"Effect of concurrent administration of granulocyte colony stimulating factor and pirfenidone on experimentally induced lung fibrosis in adult male albino rats (a histological, immunohistochemical & ultrastructural study).","authors":"Samar R Mohamed, Sally A Selim, Sanaa A Mohamed, Karima F Abdelfadeel","doi":"10.1080/01913123.2026.2668409","DOIUrl":"10.1080/01913123.2026.2668409","url":null,"abstract":"Lung fibrosis is an irreversible life-threatening condition. So, this study directed to investigate the structure of lung tissue following bleomycin-induced pulmonary injury and to evaluate the potential protective effects of pirfenidone, granulocyte colony-stimulating factor (GCSF), and their combined administration. Fifty-six adult rats were allocated into control, bleomycin-treated, pirfenidone-treated, GCSF-treated, and combination groups. Lung specimens were processed and examined using light & transmission electron microscopes beside biochemical examination for MDA and GSH. Bleomycin administration caused marked structural alterations. Both Pirfenidone and GCSF alone resulted in partial improvement of pneumocyte ultrastructure and reduced fibrotic features. Notably, the combined therapy group demonstrated the most evident protection, with near-normal pneumocyte morphology and reduced degenerative changes. These findings suggest that combined pirfenidone and GCSF therapy exerts a synergistic effect in ameliorating bleomycin-induced lung injury at the ultrastructural level.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"248-267"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148017638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The utility of electron microscopy in dermatopathology: cutaneous SMARCA4-deficient dedifferentiated large cell neuroendocrine carcinoma. 电子显微镜在皮肤病理学中的应用：皮肤smarca4缺陷去分化大细胞神经内分泌癌。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-09-09 DOI: 10.1080/01913123.2026.2726907

Katrina J Jiang, Jawaria Hassan, Daisy E Escobar, J Harrison Howard, Guillermo A Herrera, Kurt J Knowles

{"title":"The utility of electron microscopy in dermatopathology: cutaneous SMARCA4-deficient dedifferentiated large cell neuroendocrine carcinoma.","authors":"Katrina J Jiang, Jawaria Hassan, Daisy E Escobar, J Harrison Howard, Guillermo A Herrera, Kurt J Knowles","doi":"10.1080/01913123.2026.2726907","DOIUrl":"10.1080/01913123.2026.2726907","url":null,"abstract":"We report two cases of SMARCA4-deficient LCNEC (SD-LCNEC) presenting as cutaneous masses: an asymptomatic 3 cm right flank subcutaneous nodule in a 59-year-old male with concurrent pulmonary mass and metastases to cervical lymph node, and a painful 5 × 10 cm ulcerated left perineal mass in a 56-year-old female with hidradenitis suppurativa and concurrent pulmonary small cell carcinoma. To date, SD-LCNEC has been described almost exclusively as a thoracic malignancy. While various SMARCA4-deficient undifferentiated neoplasms (SD-UMN) have been identified in the skin, none have been definitively characterized as neuroendocrine. In both cases, neuroendocrine marker expression was markedly reduced on immunohistochemistry, precluding IHC-based classification as LCNEC. Electron microscopy performed on formalin-fixed paraffin-embedded tissue demonstrated abundant membrane-bound dense-core neurosecretory granules and well-formed desmosomes in both cases, ultrastructurally confirming the diagnosis. To our knowledge, these are the first reported cases of cutaneous SD-LCNEC confirmed by electron microscopy.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"522-528"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148921064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0