Ultrastructural Pathology最新文献_第4页

Aggressive angiomyxoma: an ultrastructural study update of five cases. 侵袭性血管粘液瘤：5例的超微结构研究进展。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-25 DOI: 10.1080/01913123.2025.2508401

Abigail Martínez-Jerónimo, Jazmín Itzayana Salazar-Leal, Guillermo Ernesto Corredor-Alonso, Hugo Domínguez-Malagón

引用次数: 0

Sarcomatoid mesothelioma vs. myogenic sarcoma: a strong case for diagnostic electron microscopy: a case report. 肉瘤样间皮瘤与肌源性肉瘤：电子显微镜诊断的有力案例：1例报告。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-25 DOI: 10.1080/01913123.2025.2505165

Nadine H Oury, Katherine Killian D O, Tim D Oury

{"title":"Sarcomatoid mesothelioma vs. myogenic sarcoma: a strong case for diagnostic electron microscopy: a case report.","authors":"Nadine H Oury, Katherine Killian D O, Tim D Oury","doi":"10.1080/01913123.2025.2505165","DOIUrl":"10.1080/01913123.2025.2505165","url":null,"abstract":"Mesothelioma is often considered a difficult diagnosis due to its rarity, the wide variety of histological patterns and the propensity of metastasis from cancers of unknown origin to serosal surfaces. The advent of numerous new immunochemical markers has provided extensive aid in diagnosing epithelial mesotheliomas. However, immunochemical markers that assist in the diagnosis of sarcomatoid mesothelioma remain limited. Sarcomatoid mesothelioma is the most aggressive and least common form of mesothelioma. Therefore, sarcomatoid mesothelioma diagnosis has the added challenge of increased rarity in addition to a lack of distinctive immunochemical features. Electron microscopy (EM) is a useful tool for visualizing the ultrastructural components of different tumors and has been utilized to identify distinctive features in the diagnosis of epithelial mesotheliomas. Utilization of EM in cases of sarcomatoid mesotheliomas has been limited due to a lack of diagnostic ultrastructural markers. However, EM can still be useful in evaluation of sarcomatoid tumors when sarcomatoid mesotheliomas are part of the differential diagnosis. Here, we present the case of an individual with suspected sarcomatoid mesothelioma and demonstrate the utility of EM in differentiating alternative sarcomatoid malignancies, namely a myogenic sarcoma, by identifying diagnostic ultrastructural components.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"457-461"},"PeriodicalIF":1.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study. 杨梅素改善过氧化氢诱导的人间充质干细胞氧化应激的影响：一项超微结构和免疫细胞化学研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-17 DOI: 10.1080/01913123.2025.2494618

Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta

{"title":"Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study.","authors":"Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta","doi":"10.1080/01913123.2025.2494618","DOIUrl":"https://doi.org/10.1080/01913123.2025.2494618","url":null,"abstract":"The development of new strategies to raise the survival and viability of transplanted mesenchymal stem cells (MSCs) is very important for the therapeutic potential of stem cells. The natural flavonoid myricetin has anticancer, antioxidant, anti-inflammatory and antiapoptotic effects. The effects of myricetin on human umbilical cord-derived MSCs (HUC-MSCs) induced oxidative stress with hydrogen peroxide (H2O2) were evaluated by transmission electron microscopy (TEM) and immunocytochemistry (ICC) staining. Myricetin showed an increase in the number of live cells, a decrease in caspase-3 and tumor necrosis factor-α (TNF-α) ICC staining intensity, an increase in the translocase of the mitochondrial inner membrane 17 (TIM17) ICC staining intensity, and a decrease in degeneration of cell ultrastructure in TEM against oxidative stress damage in HUC-MSCs. The results suggest that myricetin prevents oxidative stress-induced apoptosis and inflammation in the HUC-MSCs. Myricetin can be combined with HUC-MSCs in cell culture and considered as a supportive alternative treatment option.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"257-264"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of olanzapine on testes of adult albino rats and the possible role of granulocyte colony stimulating factor versus umbelliferone light and electron microscopic study. 奥氮平对成年白化病大鼠睾丸的影响及粒细胞集落刺激因子对伞蛋白的作用。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-24 DOI: 10.1080/01913123.2025.2495159

Maha A Khattab, Samah M Ahmed, Haidy G Salama, Noura H Mekawy

{"title":"Effect of olanzapine on testes of adult albino rats and the possible role of granulocyte colony stimulating factor versus umbelliferone light and electron microscopic study.","authors":"Maha A Khattab, Samah M Ahmed, Haidy G Salama, Noura H Mekawy","doi":"10.1080/01913123.2025.2495159","DOIUrl":"10.1080/01913123.2025.2495159","url":null,"abstract":"Olanzapine (OLZ) is one of atypical antipsychotic drugs (second generation) used for treating schizophrenia, manic, and mixed episodes of bipolar disorder. Continuous evaluation of its effects is necessary, and there is a need to explore alternative natural products as G-CSF and UMB to manage potential side effects. This research designed to mitigate the atypical antipsychotic drugs' adverse effects through biochemical analyses, light and electron microscopic studies. Fifty-six rats were divided into five groups: Control, OLZ, G-CSF, UMB, and Recovery groups. End body and testicular weights, serum testosterone, testicular MDA levels, and seminal analysis were recorded. Testicular specimens were processed to evaluate histological structure, PCNA, and CD34 immune expression. Morphometric and statistical analyses were also performed. OLZ group exhibited a distorted testicular structure, a significant increase in end body and a decline in testicular weight, a significant decline in the serum level of testosterone level, testicular MDA, and seminal analysis parameters. Furthermore, disturbed histoarchitecture, reduction in PCNA, and elevation in CD34 immunoreaction were observed. These alterations were partially attenuated by G-CSF therapy, whereas UMB significantly improved all parameters. In conclusion, UMB, and to a lesser degree G-CSF, appeared to be superior therapeutic options by attenuating oxidative stress and restoring intact histological structure and biochemical parameters.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"265-287"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic lymphocytic leukemia with associated renal invasion and light chain-restricted deposition membranous nephropathy: a case report and literature review. 慢性淋巴细胞白血病合并肾侵犯和轻链限制性沉积膜性肾病1例报告并文献复习。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-25 DOI: 10.1080/01913123.2025.2506742

Chong Geng, Hongmin Luo

{"title":"Chronic lymphocytic leukemia with associated renal invasion and light chain-restricted deposition membranous nephropathy: a case report and literature review.","authors":"Chong Geng, Hongmin Luo","doi":"10.1080/01913123.2025.2506742","DOIUrl":"10.1080/01913123.2025.2506742","url":null,"abstract":"Chronic lymphocytic leukemia (CLL) is a prevalent hematological malignancy that significantly affects the kidneys as an extramedullary organ. Reports from autopsy studies have shown the infiltration of CLL cells into the renal parenchymal in 63-93% of cases. Glomerular diseases associated with CLL are relatively rare, occurring in approximately 2% of patients and often presenting as nephrotic syndrome. The most common histological pattern observed in CLL-associated glomerular diseases is membranoproliferative glomerulonephritis, followed by minimal change disease and membranous nephropathy. In this report, we presented a case of a 69-year-old male patient with CLL who developed nephrotic syndrome. The diagnosis of CLL was confirmed through bone marrow and renal biopsies, which revealed the presence of CLL tumor cells in the renal interstitium along with membranous nephropathy characterized by light chain-restricted deposits. The tumor cells present in the renal interstitium and glomeruli of the patient expressed identical light chain restrictions, which suggested that the membranous nephropathy was secondary and possibly induced by the deposition of tumor-associated antigens. Treatment with a combination of fludarabine, cyclophosphamide, and rituximab led to the remission of both the CLL and nephrotic syndrome, with no recurrence observed during the follow-up period.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"377-383"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light chain crystalline podocytopathy caused by electron-lucent crystals - a diagnostic challenge: a case report. 由电子发光晶体引起的轻链结晶足细胞病——一个诊断挑战：1例报告。

IF 1.2 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-07-18 DOI: 10.1080/01913123.2025.2535622

Rajib K Gupta, Maria E Pagtalunan, Ann M Wexler, Joseph M Tuscano

引用次数: 0

Primary ciliary dyskinesia cases bronchoscopic sampling and TEM analysis: sampling & diagnosis in PCD. 原发性纤毛运动障碍病例的支气管镜取样与透射电镜分析：PCD的取样与诊断。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-30 DOI: 10.1080/01913123.2025.2498418

Deniz Doğan Mülazimoğlu, Oya Evirgen, Oya Kayacan, Demet Karnak

{"title":"Primary ciliary dyskinesia cases bronchoscopic sampling and TEM analysis: sampling & diagnosis in PCD.","authors":"Deniz Doğan Mülazimoğlu, Oya Evirgen, Oya Kayacan, Demet Karnak","doi":"10.1080/01913123.2025.2498418","DOIUrl":"10.1080/01913123.2025.2498418","url":null,"abstract":"Primary ciliary dyskinesia (PCD) is a rare, genetically heterogeneous disorder characterized by impaired ciliary structure and function, leading to chronic respiratory symptoms and recurrent infections. Despite its clinical significance, PCD diagnosis remains challenging due to its variable presentation and the lack of a gold standard diagnostic test. Specific clinical criteria, including neonatal respiratory distress and laterality defects, aid in suspicion of PCD, but confirmatory diagnosis often requires a combination of tests. In this study, we aimed to assess the efficacy of bronchoscopic techniques in obtaining respiratory epithelial samples for transmission electron microscopy (TEM) analysis. We enrolled adults with bronchiectasis and suspected PCD who underwent fiberoptic bronchoscopy. Bronchial forceps and brush biopsies were obtained from specific bronchial segments under conscious sedation. Tissue samples were processed for TEM analysis to identify ultrastructural axonemal defects associated with PCD. Our study included 10 patients (3 females, 7 males) aged 19-38 years, with detailed demographics and clinical characteristics provided. Evaluation of tracheobronchial biopsy samples revealed higher histological scores for the presence of ciliated cells and transverse sections of cilia in pellets obtained from brush biopsies and fixative solutions of forceps biopsy compared to forceps biopsy tissue samples. Electron microscopic examination of ultra-thin sections demonstrated abundant ciliated cells and abnormal cilia structures, aiding in the diagnosis of PCD in pellets. PCD represents a significant etiology of bronchiectasis, emphasizing the need for accurate diagnosis and appropriate management strategies. Our findings highlight the importance of bronchoscopic techniques, including bronchial brushing alongside forceps biopsies, in enhancing diagnostic yield and guiding timely intervention to improve patient outcomes.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"371-376"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric study of proximal tubular cell mitochondria using TEM images in renal diseases. 肾脏疾病近端小管细胞线粒体的透射电镜形态学研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-24 DOI: 10.1080/01913123.2025.2494621

Dibyajyoti Boruah, Varun Bajaj, Barun Kumar Chakrabarty, Sarika Pardeshi, A W Kashif, S Venkatesan

{"title":"Morphometric study of proximal tubular cell mitochondria using TEM images in renal diseases.","authors":"Dibyajyoti Boruah, Varun Bajaj, Barun Kumar Chakrabarty, Sarika Pardeshi, A W Kashif, S Venkatesan","doi":"10.1080/01913123.2025.2494621","DOIUrl":"https://doi.org/10.1080/01913123.2025.2494621","url":null,"abstract":"The kidney is rich in mitochondria, and any alterations or damage to tubular cell mitochondria play an important role in renal metabolic activities and the pathogenesis of various kidney diseases. Quantitative analysis of mitochondrial concentration, size, and shape is essential for understanding mitochondrial biology in renal disorders. This study assessed mitochondrial morphometric parameters of the proximal convoluted tubular cell adjacent to the glomerulus in different renal disorders and investigated how they correlated with serum creatinine. A total of 65 kidney biopsy cases received by the transmission electron microscope (TEM) laboratory for diagnosis were included in the study. TEM images of glutaraldehyde-osmium tetroxide fixed epoxy-resin embedded 70 nm thick sections were used for the evaluation of (i) minor axis(MinX) (ii) major axis(MajX) (iii) Area, (iv)Perimeter, (v) Aspect ratio and (vi) Roundness of mitochondria in renal tubular cells using QuPath software. Mitochondrial density (MDensity), % of mitochondrial space (MSpace), and mitochondrial surface density (MSDensity) in the cytoplasm of tubular space were estimated for each sample. Serum creatinine showed good negative correlations with MSpace and MSDensity, and elongation of mitochondria was more in renal disorder in comparison to normal histology, which indicated the variation of mitochondrial concentration and shape in proximal tubular cells could be important features in the renal function disorder.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"49 3","pages":"315-325"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperlipidemia and endothelial dysfunction: effects of eNOS phosphorylation and vascular ultrastructure in normoglycemic Psammomys obesus. 高脂血症和内皮功能障碍：eNOS磷酸化和血管超微结构对血糖正常的肥胖鼠的影响。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-24 DOI: 10.1080/01913123.2025.2510390

Mohamed El Fadel Ousmaal, Ramaroson Andriantsitohaina, Nafila Zouaghi, Jean Giaimis, M Carmen Martínez, Ahsene Baz

{"title":"Hyperlipidemia and endothelial dysfunction: effects of eNOS phosphorylation and vascular ultrastructure in normoglycemic Psammomys obesus.","authors":"Mohamed El Fadel Ousmaal, Ramaroson Andriantsitohaina, Nafila Zouaghi, Jean Giaimis, M Carmen Martínez, Ahsene Baz","doi":"10.1080/01913123.2025.2510390","DOIUrl":"10.1080/01913123.2025.2510390","url":null,"abstract":"Atherosclerosis represents the most prevalent form of cardiovascular disease, with the potential to ultimately result in clinically significant complications such as myocardial infarction and stroke. The objective of our study was to gain a deeper understanding of the independent role of hyperlipidemia in the development of endothelial dysfunction and ultrastructural damage to the arteries, which is a key factor in the pathogenesis of atherosclerosis. Following a 12-week dietary intervention comprising either a high-energy diet (HED) or a normal diet (ND), fasting plasma glucose and lipid parameters were assessed. The aortas were subjected to histological analysis and Western blotting, while the carotid arteries underwent ultrastructural analysis using transmission electron microscopy. HED resulted in a statistically significant elevation in lipid parameters, even in normoglycemic P. obesus. eNOS, phospho-eNOS (Thr 495), and NF-κB p65 protein expression were increased in the aorta of HED-fed P. obesus. Histological examination and ultrastructural analysis of HED-fed P. obesus demonstrated notable vascular remodeling, manifested by segmental arterial wall thickening and the presence of large vacuoles and lipid droplets in endothelial cells. This study provides evidence that hyperlipidemia is a significant contributing factor to endothelial dysfunction and ultrastructural alterations in blood vessels, even in the absence of severe hyperglycemia.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"354-370"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural alterations of hepatocytes in short-lived model fish, Nothobranchius furzeri Jubb, 1971. 短寿命模型鱼肝细胞超微结构的改变，nothobranchus furzeri Jubb, 1971。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-25 DOI: 10.1080/01913123.2025.2504424

Iva Dyková, Jakub Žák, Radim Blažek, Ondřej Slabý

引用次数: 0