Mohamed El Fadel Ousmaal, Ramaroson Andriantsitohaina, Nafila Zouaghi, Jean Giaimis, M Carmen Martínez, Ahsene Baz
{"title":"高脂血症和内皮功能障碍:eNOS磷酸化和血管超微结构对血糖正常的肥胖鼠的影响。","authors":"Mohamed El Fadel Ousmaal, Ramaroson Andriantsitohaina, Nafila Zouaghi, Jean Giaimis, M Carmen Martínez, Ahsene Baz","doi":"10.1080/01913123.2025.2510390","DOIUrl":null,"url":null,"abstract":"<p><p>Atherosclerosis represents the most prevalent form of cardiovascular disease, with the potential to ultimately result in clinically significant complications such as myocardial infarction and stroke. The objective of our study was to gain a deeper understanding of the independent role of hyperlipidemia in the development of endothelial dysfunction and ultrastructural damage to the arteries, which is a key factor in the pathogenesis of atherosclerosis. Following a 12-week dietary intervention comprising either a high-energy diet (HED) or a normal diet (ND), fasting plasma glucose and lipid parameters were assessed. The aortas were subjected to histological analysis and Western blotting, while the carotid arteries underwent ultrastructural analysis using transmission electron microscopy. HED resulted in a statistically significant elevation in lipid parameters, even in normoglycemic <i>P. obesus</i>. eNOS, phospho-eNOS (Thr 495), and NF-κB p65 protein expression were increased in the aorta of HED-fed <i>P. obesus</i>. Histological examination and ultrastructural analysis of HED-fed <i>P. obesus</i> demonstrated notable vascular remodeling, manifested by segmental arterial wall thickening and the presence of large vacuoles and lipid droplets in endothelial cells. This study provides evidence that hyperlipidemia is a significant contributing factor to endothelial dysfunction and ultrastructural alterations in blood vessels, even in the absence of severe hyperglycemia.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"354-370"},"PeriodicalIF":1.2000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hyperlipidemia and endothelial dysfunction: effects of eNOS phosphorylation and vascular ultrastructure in normoglycemic <i>Psammomys obesus</i>.\",\"authors\":\"Mohamed El Fadel Ousmaal, Ramaroson Andriantsitohaina, Nafila Zouaghi, Jean Giaimis, M Carmen Martínez, Ahsene Baz\",\"doi\":\"10.1080/01913123.2025.2510390\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Atherosclerosis represents the most prevalent form of cardiovascular disease, with the potential to ultimately result in clinically significant complications such as myocardial infarction and stroke. The objective of our study was to gain a deeper understanding of the independent role of hyperlipidemia in the development of endothelial dysfunction and ultrastructural damage to the arteries, which is a key factor in the pathogenesis of atherosclerosis. Following a 12-week dietary intervention comprising either a high-energy diet (HED) or a normal diet (ND), fasting plasma glucose and lipid parameters were assessed. The aortas were subjected to histological analysis and Western blotting, while the carotid arteries underwent ultrastructural analysis using transmission electron microscopy. HED resulted in a statistically significant elevation in lipid parameters, even in normoglycemic <i>P. obesus</i>. eNOS, phospho-eNOS (Thr 495), and NF-κB p65 protein expression were increased in the aorta of HED-fed <i>P. obesus</i>. Histological examination and ultrastructural analysis of HED-fed <i>P. obesus</i> demonstrated notable vascular remodeling, manifested by segmental arterial wall thickening and the presence of large vacuoles and lipid droplets in endothelial cells. 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Hyperlipidemia and endothelial dysfunction: effects of eNOS phosphorylation and vascular ultrastructure in normoglycemic Psammomys obesus.
Atherosclerosis represents the most prevalent form of cardiovascular disease, with the potential to ultimately result in clinically significant complications such as myocardial infarction and stroke. The objective of our study was to gain a deeper understanding of the independent role of hyperlipidemia in the development of endothelial dysfunction and ultrastructural damage to the arteries, which is a key factor in the pathogenesis of atherosclerosis. Following a 12-week dietary intervention comprising either a high-energy diet (HED) or a normal diet (ND), fasting plasma glucose and lipid parameters were assessed. The aortas were subjected to histological analysis and Western blotting, while the carotid arteries underwent ultrastructural analysis using transmission electron microscopy. HED resulted in a statistically significant elevation in lipid parameters, even in normoglycemic P. obesus. eNOS, phospho-eNOS (Thr 495), and NF-κB p65 protein expression were increased in the aorta of HED-fed P. obesus. Histological examination and ultrastructural analysis of HED-fed P. obesus demonstrated notable vascular remodeling, manifested by segmental arterial wall thickening and the presence of large vacuoles and lipid droplets in endothelial cells. This study provides evidence that hyperlipidemia is a significant contributing factor to endothelial dysfunction and ultrastructural alterations in blood vessels, even in the absence of severe hyperglycemia.
期刊介绍:
Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology.
Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics:
Advances in the uses of electron microscopic and immunohistochemical techniques
Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis
Important new, investigative, clinical, and diagnostic EM methods.