高脂血症和内皮功能障碍:eNOS磷酸化和血管超微结构对血糖正常的肥胖鼠的影响。

IF 1.2 4区 医学 Q4 MICROSCOPY
Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-05-24 DOI:10.1080/01913123.2025.2510390
Mohamed El Fadel Ousmaal, Ramaroson Andriantsitohaina, Nafila Zouaghi, Jean Giaimis, M Carmen Martínez, Ahsene Baz
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引用次数: 0

摘要

动脉粥样硬化是最常见的心血管疾病,有可能最终导致临床显著的并发症,如心肌梗死和中风。我们的研究目的是为了更深入地了解高脂血症在动脉内皮功能障碍和超微结构损伤发展中的独立作用,这是动脉粥样硬化发病的关键因素。经过12周的饮食干预,包括高能量饮食(HED)或正常饮食(ND),评估空腹血糖和血脂参数。对主动脉进行组织学分析和Western blotting,对颈动脉进行透射电镜超微结构分析。HED导致血脂参数显著升高,即使在血糖正常的肥胖P.。eNOS、phospho-eNOS (Thr 495)、NF-κB p65蛋白表达均升高。组织学检查和超微结构分析显示,饲喂hed的肥胖大鼠血管重构明显,表现为节段性动脉壁增厚,内皮细胞内出现大空泡和脂滴。本研究证明,即使在没有严重高血糖的情况下,高脂血症也是导致血管内皮功能障碍和超微结构改变的重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hyperlipidemia and endothelial dysfunction: effects of eNOS phosphorylation and vascular ultrastructure in normoglycemic Psammomys obesus.

Atherosclerosis represents the most prevalent form of cardiovascular disease, with the potential to ultimately result in clinically significant complications such as myocardial infarction and stroke. The objective of our study was to gain a deeper understanding of the independent role of hyperlipidemia in the development of endothelial dysfunction and ultrastructural damage to the arteries, which is a key factor in the pathogenesis of atherosclerosis. Following a 12-week dietary intervention comprising either a high-energy diet (HED) or a normal diet (ND), fasting plasma glucose and lipid parameters were assessed. The aortas were subjected to histological analysis and Western blotting, while the carotid arteries underwent ultrastructural analysis using transmission electron microscopy. HED resulted in a statistically significant elevation in lipid parameters, even in normoglycemic P. obesus. eNOS, phospho-eNOS (Thr 495), and NF-κB p65 protein expression were increased in the aorta of HED-fed P. obesus. Histological examination and ultrastructural analysis of HED-fed P. obesus demonstrated notable vascular remodeling, manifested by segmental arterial wall thickening and the presence of large vacuoles and lipid droplets in endothelial cells. This study provides evidence that hyperlipidemia is a significant contributing factor to endothelial dysfunction and ultrastructural alterations in blood vessels, even in the absence of severe hyperglycemia.

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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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