Ultrastructural Pathology最新文献_第5页

Investigation the effects of 2-aminoethoxydiphenyl borate (2-APB) on aminoglycoside nephrotoxicity. 2-氨基乙氧基二苯硼酸酯(2-APB)对氨基糖苷肾毒性的影响。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2024-01-02 Epub Date: 2024-01-09 DOI: 10.1080/01913123.2023.2278629

Özge Kendirlinan, Yurdun Kuyucu, Bilge Güzelel, Ebru Dündar Yenilmez, Abdullah Tuli, Gülşah Seydaoğlu, Ufuk Özgü Mete

{"title":"Investigation the effects of 2-aminoethoxydiphenyl borate (2-APB) on aminoglycoside nephrotoxicity.","authors":"Özge Kendirlinan, Yurdun Kuyucu, Bilge Güzelel, Ebru Dündar Yenilmez, Abdullah Tuli, Gülşah Seydaoğlu, Ufuk Özgü Mete","doi":"10.1080/01913123.2023.2278629","DOIUrl":"10.1080/01913123.2023.2278629","url":null,"abstract":"Investigation the protective effect of transient receptor potential channel modulator 2-Aminoethoxydiphenyl Borate (2-APB) on aminoglycoside nephrotoxicity caused by reactive oxygen species, calcium-induced apoptosis and inflammation was aimed. Forty Wistar rats were divided (n=8) as follows: Control group; DMSO group; 2-APB group; Gentamicin group (injected 100 mg/kg gentamicin intramuscularly for 10 days); Gentamicin+ 2-APB group (injected 2 mg/kg 2-APB intraperitoneally, then after 30 minutes 100 mg/kg gentamicin was injected intramuscularly for 10 days). Blood samples were collected for biochemical analyses, kidney tissue samples were collected for light, electron microscopic and immunohistochemical investigations. In gentamicin group glomerular degeneration, tubular dilatation, vacuolization, desquamation of tubular cells and hyaline cast formation in luminal space and leukocyte infiltration were seen. Disorganization of microvilli of tubular cells, apical cytoplasmic blebbing, lipid accumulation, myelin figure like structure formation, increased lysosomes, mitochondrial swelling and disorganization of cristae structures, apoptotic changes and widening of intercellular space were found. TNF-α, IL-6 and caspase 3 expressions were increased. BUN and creatinine concentrations were increased. Increase in MDA levels and decrease in SOD activities were determined. Even though degeneration still continues in gentamicin+2-APB treatment group, severity and the area it occupied were decreased and the glomerular and tubule structures were generally preserved. TNF-α, IL-6, caspase 3 immunoreactivities and BUN, creatinine, MDA concentrations were reduced and SOD activities were increased markedly compared to gentamicin group. In conclusion, it has been considered that 2-APB can prevent gentamicin mediated nephrotoxicity with its anti-oxidant, anti-apoptotic and anti-inflammatory effects.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"29-41"},"PeriodicalIF":1.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134649867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular organelles remodeling in myocardial endotheliocytes in COVID-19: an autopsy-based study. COVID-19患者心肌内皮细胞胞内细胞器重构:一项基于尸检的研究

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2024-01-02 Epub Date: 2024-01-09 DOI: 10.1080/01913123.2023.2286977

Nataliya Bgatova, Sergey Savchenko, Alexei Lamanov, Iuliia Taskaeva, Boris Ayzikovich, Valentina Gritcinger, Andrey Letyagin, Maksim Korolev

{"title":"Intracellular organelles remodeling in myocardial endotheliocytes in COVID-19: an autopsy-based study.","authors":"Nataliya Bgatova, Sergey Savchenko, Alexei Lamanov, Iuliia Taskaeva, Boris Ayzikovich, Valentina Gritcinger, Andrey Letyagin, Maksim Korolev","doi":"10.1080/01913123.2023.2286977","DOIUrl":"10.1080/01913123.2023.2286977","url":null,"abstract":"It is known that the unfavorable outcome in patients infected with SARS-CoV-2 may be associated with the development of complications caused by heart damage due to the direct virus action. The mechanism of these cardiovascular injuries caused by SARS-CoV-2 infection has not been fully understood; however, the study of COVID-19-associated myocardial microcirculatory dysfunction can represent the useful strategy to solving this challenge. Thus, here we aimed to study the ultrastructural organization of endothelial cells of myocardial capillaries in patients with COVID-19. The morphology of endotheliocytes of the myocardial blood capillaries in patients with COVID-19 was studied on cardiac autopsy material using transmission electron microscopy. The endotheliocytes of myocardial capillaries in patients with COVID-19 were characterized by the abundant rough endoplasmic reticulum (ER) membranes, the Golgi complex, and free polysomal complexes of ribosomes and lipids. The presence of double membrane vesicles with virions and zippered ER was detected in the cytoplasm of endotheliocytes. The revealed endothelial ultrastructural changes indicate the remodeling of intracellular membranes during SARS-CoV-2 infection. Our findings confirm the formation of virus-induced structures in myocardial endothelial cells considered critical for viral replication and assembly. The data may elucidate the mechanisms of endothelial dysfunction development in patients with COVID-19 to provide potential targets for drug therapy.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"66-74"},"PeriodicalIF":1.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myxoinflammatory fibroblastic sarcoma: ultrastructural study of 7 cases 肌炎性纤维肉瘤：7 个病例的超微结构研究

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-12-21 DOI: 10.1080/01913123.2023.2292559

Guillermo Ernesto Corredor-Alonso, Claudia Haydee Sarai Caro-Sánchez, Hugo Ricardo Domínguez-Malagón

引用次数: 0

Punctate IgG staining particles localize in the budding ballooning clusters of reactive foot processes in minimal change disease 点状 IgG 染色颗粒定位在极小病变中反应性足部过程的萌芽气球团中

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-12-14 DOI: 10.1080/01913123.2023.2292590

Ping L. Zhang, Vasudevan D. Mahalingam, Brandon D. Metcalf, Yazan A. Al-Othman, Wei Li, Hassan D. Kanaan, Guillermo A. Herrera

引用次数: 0

Comparative effects of chemical and green zinc oxide nanoparticles in caprine testis: ultrastructural and steroidogenic enzyme analysis 化学纳米颗粒和绿色纳米氧化锌对黄羊睾丸影响的比较：超微结构和类固醇生成酶分析

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-12-12 DOI: 10.1080/01913123.2023.2286963

Sneha Bareja, Rajnesh Kumar Sharma

引用次数: 0

The Protective effects of Vitamin E against alterations of rat testis structure induced by deltamethrin; histological, ultrastructure, and biochemical study 维生素 E 对溴氰菊酯引起的大鼠睾丸结构改变的保护作用；组织学、超微结构和生化研究

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-12-10 DOI: 10.1080/01913123.2023.2292563

Ghada Nady Ouais, Sherif A. Kamar, Ali Ahmed Mousa, Mohamed Mostafa Sonbol

引用次数: 0

The possible protective role of selenium on the visual cortex of adult albino rat on exposure to potassium dichromate. 硒对重铬酸钾暴露于成年白化病大鼠视觉皮层的可能保护作用。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-11-30 DOI: 10.1080/01913123.2023.2259455

Sally S Anwer, Hala Mohamed Hassanin

引用次数: 0

Adverse neurological effects after exposure to copper, manganese, and mercury mixtures in a Spraque-Dawley rat model: an ultrastructural investigation. Spraque-Dawley大鼠模型暴露于铜、锰和汞混合物后的神经系统不良反应：超微结构研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-11-30 DOI: 10.1080/01913123.2023.2270580

Maxine Draper, Megan Jean Bester, Mia-Jeanne Van Rooy, Hester Magdalena Oberholzer

{"title":"Adverse neurological effects after exposure to copper, manganese, and mercury mixtures in a Spraque-Dawley rat model: an ultrastructural investigation.","authors":"Maxine Draper, Megan Jean Bester, Mia-Jeanne Van Rooy, Hester Magdalena Oberholzer","doi":"10.1080/01913123.2023.2270580","DOIUrl":"10.1080/01913123.2023.2270580","url":null,"abstract":"Exposure to environmental metal pollutants is linked to oxidative stress and the subsequent development of neurological disease. In this study, the effects of copper, manganese, and mercury, were evaluated at X100 the World Health Organization safety limits for drinking water. Using a Sprague-Dawley rat model, following exposure for 28 days, the effects of these metals on biochemical blood parameters and tissue and cellular structure of the brain were determined. Biochemical analysis revealed no hepatocellular injury with minor changes associated with the hepatobiliary system. Minimal changes were found for renal function and the Na+/K+ ratio was reduced in the copper and manganese (Cu + Mn) and copper, manganese, and mercury (Cu, Mn + Hg) groups that could affect neurological function. Light microscopy of the brain revealed abnormal histopathology of Purkinje cells in the cerebellum and pyramidal cells in the cerebrum as well as tissue damage and fibrosis of the surface blood vessels. Transmission electron microscopy of the cerebral neurons showed microscopic signs of axonal damage, chromatin condensation, the presence of indistinct nucleoli and mitochondrial damage. Together these cellular features suggest the presence and influence of oxidative stress. Exposure to these metals at X100 the safety limits, as part of mixtures, induces changes to neurological tissue that could adversely influence neurological functioning in the central nervous system.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"509-528"},"PeriodicalIF":1.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41238993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Sonic hedgehog pathway inhibitor GDC0449 induces autophagic death in human Medulloblastoma Daoy cells. Sonic hedgehog通路抑制剂GDC0449诱导人髓母细胞瘤day细胞自噬死亡。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-11-30 DOI: 10.1080/01913123.2023.2270676

Qi Zhang, Wanjing Zou, Longtao He, Cuiping Zhang, Ying Wang

{"title":"The Sonic hedgehog pathway inhibitor GDC0449 induces autophagic death in human Medulloblastoma Daoy cells.","authors":"Qi Zhang, Wanjing Zou, Longtao He, Cuiping Zhang, Ying Wang","doi":"10.1080/01913123.2023.2270676","DOIUrl":"10.1080/01913123.2023.2270676","url":null,"abstract":"Medulloblastoma (MB) is a frequently occurring malignant brain tumor in children, and many of these tumors are identified by the abnormal activation of the Sonic Hedgehog (SHH) pathway. Although the Shh inhibitor GDC0449 initially shows some effectiveness in certain tumors, they eventually recur due to drug resistance mechanisms, highlighting the need for new treatment options. In this study, we explore whether GDC0449 induces autophagy in the human MB cell lines. To investigate the ultrastructural pathology changes of GDC0449-treated Daoy and D283 cells, we employed Transmission Electron Microscopy (TEM) technology to identify the expression of autophagic vacuoles. Our results indicate that GDC0449 only increases autophagy in Daoy cells by increasing the LC3-II/LC3-I ratio and autophagosome formation.We also analyzed Beclin1, LC3, Bax, and Cleaved-caspase3 protein and mRNA expression levels of autophagic and apoptotic markers using fluorescence confocal microscopy, RT-PCR, and Western blot. We found that cell autophagy and apoptosis increased in a dose-dependent manner with GDC0449 treatment. Additionally, we observed increased mammalian target of rapamycin (mTOR) phosphorylation and decreased protein kinase B (AKT/PKB), Ribosomal Protein S6, eIF4E-binding protein (4EBP1) phosphorylation in GDC0449-treated Daoy cells. It was observed that inhibiting autophagy using Beclin1 siRNA significantly blocked the apoptosis-inducing effects of GDC0449, suggesting that GDC0449 mediates its apoptotic effects by inducing autophagy.Our data suggests that GDC0449 inhibits the growth of human MB Daoy cells by autophagy-mediated apoptosis. The mechanism of GDC0449-induced autophagy in Daoy cells may be related to the inhibition of the PI3K/AKT/mTOR signaling pathway.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"529-539"},"PeriodicalIF":1.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of small leucine-rich proteoglycans and telocytes in thin and thick human endometrium: immunohistochemical and ultrastructural examination. 富含亮氨酸的小蛋白多糖和端粒细胞在薄和厚人类子宫内膜中的参与：免疫组织化学和超微结构检查。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-11-30 DOI: 10.1080/01913123.2023.2270660

Ozlem Karabay Akgul, Tugba Ekiz-Yilmaz

{"title":"Involvement of small leucine-rich proteoglycans and telocytes in thin and thick human endometrium: immunohistochemical and ultrastructural examination.","authors":"Ozlem Karabay Akgul, Tugba Ekiz-Yilmaz","doi":"10.1080/01913123.2023.2270660","DOIUrl":"10.1080/01913123.2023.2270660","url":null,"abstract":"Thin endometrium, defined as an endometrial thickness of less than 7 mm during the late follicular phase, is a common cause of frequent cancelation of embryo transfers or recurrent implantation failure during assisted reproductive treatment. Small proteoglycans regulate intracellular signaling cascades by bridging other matrix molecules and tissue elements, affecting cell proliferation, adhesion, migration, and cytokine concentration. The aim of the study is to investigate the role of small leucine-rich proteoglycans in the pathogenesis of thin and thick human endometrium and their differences from normal endometrium in terms of fine structure properties. Normal, thin, and thick endometrial samples were collected, and small leucine-rich proteoglycans (SLRPs), decorin, lumican, biglycan, and fibromodulin immunoreactivities were comparatively analyzed immunohistochemically. The data were compared statistically. Moreover, ultrastructural differences among the groups were evaluated by transmission electron microscopy. The immunoreactivities of decorin, lumican, and biglycan were higher in the thin endometrial glandular epithelium and stroma compared to the normal and thick endometrium (p < .001). Fibromodulin immunoreactivity was also higher in the thin endometrial glandular epithelium than in the normal and thick endometrium (p < .001). However, there was no statistical difference in the stroma among the groups. Ultrastructural features were not profoundly different among cases. Telocytes, however, were not seen in the thin endometrium in contrast to normal and thin endometrial tissues. These findings suggest a possible role of changes in proteoglycan levels in the pathogenesis of thin endometrium.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"484-494"},"PeriodicalIF":1.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41238994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0