Ultrastructural Pathology最新文献

{"title":"Therapeutic potential of mesenchymal stem cells-derived exosomes on experimentally induced neurodegeneration in olfactory bulb of adult male albino rat: histological, physiological and biochemical studies.","authors":"Mayada M Elkeblawy, Mona Tayssir Sadek, Azza A M Abouraia, Eman M El-Beltagi","doi":"10.1080/01913123.2026.2616037","DOIUrl":"10.1080/01913123.2026.2616037","url":null,"abstract":"<p><p>Neurodegeneration (ND) is a major medical problem in elderly. Olfactory bulb represents an early-vulnerable brain area to ND. Mesenchymal stem cells-derived exosomes are currently evaluated as a possible therapeutic agent for several diseases. This study aimed to assess exosomes-therapeutic potential on ND-induced olfactory bulb changes. Forty-five adult male albino rats were equally categorized into three groups: control (I), ND (II), and exosomes treated (III). ND was induced in groups II and III using aluminum chloride, then group III rats received a single intravenous injection of 200 µg exosomes. Buried food test was conducted to assess sense of olfaction. Blood was collected to assess serum malondialdehyde (MDA) and total antioxidant capacity (TAC) levels. Olfactory bulb specimens were prepared for histological and immunohistochemical (GFAP & synaptophysin) studies. ND group displayed a highly significant increase in buried food test time and MDA level, along with decrease in TAC level. Marked histological changes were observed affecting nerve cells, synapses, neuropil and bulb vasculature with a highly significant increase in GFAP area percentage and decrease in that of synaptophysin. Treatment with exosomes in group III markedly ameliorated these changes. These results reflected that MSCs-derived exosomes had a remarkable therapeutic potential against ND-induced changes in olfactory bulb.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"114-133"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological, immunohistochemical, and ultrastructural evaluation of mesenchymal stem cell-derived microvesicles versus granulocyte colony-stimulating factor in jejunal ischemia-reperfusion injury in albino rats.","authors":"Heba M Abdel-Aziz, Aya A Mahmoud, Maha Z Mohammed, Hala M Soliman","doi":"10.1080/01913123.2026.2643583","DOIUrl":"10.1080/01913123.2026.2643583","url":null,"abstract":"<p><p>Intestinal ischemia-reperfusion injury (IIRI) is a life-threatening vascular emergency with high mortality, commonly occurring as a complication of hemorrhagic shock or acute mesenteric arterial occlusion. When revascularization is performed after this issue, injury will occur. The influence of MSC-MVs and G-CSF on IIRI was detected in this study. Fifty-six male adult albino rats were allocated into five groups: control, ischemia-reperfusion, MSC-MVs, G-CSF, and recovery. Ischemic injury was induced for one hour using micro-vascular clamp across the superior mesenteric artery at its beginning from the aorta. After that, the clamp was withdrawn to allow reperfusion for two hours. At the end of experimental period, rats were sacrificed. Jejunal tissues were processed for assessment of oxidative stress markers (MDA and SOD) as well as for histological and immune histo-chemical analysis. Ischemia-reperfusion group revealed highly statistically significant increase in tissue MDA while SOD showed the reverse, histologically by light microscopic examination; inflammatory cellular infiltration, congested blood vessels, and separated muscle fibers in muscularis mucosa were observed. Ultrastructurally, absorptive columnar cells appeared with heterochromatic nuclei, lost apical microvilli, and widen intercellular space. These findings showed more improvement in MSC-MVs group in comparison with G-CSF group while recovery group exhibited severe affection.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"134-158"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147499755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentially expressed genes and pathways in glomeruli in light chain deposition disease - LCDD (monoclonal immunoglobulin deposition disease): opportunities for novel therapeutic interventions.","authors":"Guillermo A Herrera, Jiamin Teng, Chun Zeng, Luis Del Pozo-Yauner, Elba A Turbat-Herrera","doi":"10.1080/01913123.2026.2721624","DOIUrl":"10.1080/01913123.2026.2721624","url":null,"abstract":"<p><p>The future of organ-based therapies in monoclonal light chain-associated renal diseases is centered on the utilization of techniques that can identify specific targets to inhibit initiation and/or control progression. The present study of renal biopsies of patients with light chain deposition disease in different stages of glomerular involvement using spatial transcriptomics revealed unique gene expressions and activation of cellular pathways in mesangial cells responsible for the initiation and progression of the disease processes that can be targeted pharmacologically. Among the over-expressed genes were RPL41, RPS26, ACTA2-actin protein, TNC, and TGF (transforming growth factor) β1. The RPS and RPL genes regulate ribosomal function, their increase resulting in accentuated production of proteins. TGF-β drives matrix formation by mesangial cells, TNC is the gene responsible for the tenascin-rich mesangial nodules, and ACTA2 is an actin protein gene in myofibroblastic cells (phenotypically transformed mesangial cells), resulting in increased and cytoplasmic relocalization of actin in mesangial cells, leading to surface ruffling to promote interactions of monoclonal light chains with the SORL1 receptor. The pathways activated included the eukaryotic translation initiator, the extracellular matrix/proteoglycans and organization, and the TGF-β1 signaling, all aimed at increasing the extracellular matrix in the mesangium, responsible for its nodularity. The genes and cellular pathways differed in the various stages of glomerular LCDD, emphasizing the need for designing personalized therapies based on the evaluation of renal biopsies. Additional laboratory testing using existing models of LCDDs should be utilized to prove the efficacy of the proposed targeted interventions.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"499-515"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary Renal Neuroendocrine Tumors Presenting as a Complex Cystic Mass and Arising in a Horseshoe Kidney: A Report of Two Cases.","authors":"Bhawana Dhiman, Hemlata Jangir, Raghuraman Soundararajan, Atul Kumar Jain, Amanjit Bal, Ravi Mohan","doi":"10.1080/01913123.2026.2724937","DOIUrl":"10.1080/01913123.2026.2724937","url":null,"abstract":"<p><p>Primary renal neuroendocrine tumors (PRNETs) are exceptionally rare neoplasms that account for less than 1% of genitourinary neuroendocrine neoplasms and pose considerable diagnostic difficulty because of their non-specific clinicoradiological presentations and diverse histomorphology, which frequently mimics conventional renal epithelial tumors. We describe two adult patients with well-differentiated PRNET managed by partial nephrectomy including a 41-year-old male with a complex bosniak category IV cystic renal lesion, and another 43-year-old female with a tumor arising from the isthmus of a horse-shoe kidney. Both tumors showed organoid, trabecular, nested, tubulocystic, and focal cribriform architecture with low mitotic activity. Case 1 additionally showed focal capsular and perineural invasion despite of low-grade morphology. Tumor cells were diffusely positive for synaptophysin, chromogranin, and CD99, and negative for PAX8, CK7, CK20, WT1, TTF1, and CDX2, with a Ki-67 index below 2% in both. Electron microscopy confirmed dense-core neurosecretory granules, and both patients remain disease-free on radioimaging based follow-up. These cases illustrate that PRNET should be considered whenever a renal mass, including a complex cystic lesion or arising in a congenital anomalous kidney, not confirming thetypical epithelial morphology, and a combined histomorphological, immunohistochemical, and ultrastructural approach with prolonged surveillance is essential for accurate diagnosis and management.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"516-521"},"PeriodicalIF":1.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of DNA damage and growth arrest by citalopram in breast cancer cells mediated via activation of Gadd45a and apoptotic genes.","authors":"Mohammed Salama, Ahmed Elamin, Magda Youssif, Noura A Mattar","doi":"10.1080/01913123.2025.2454691","DOIUrl":"10.1080/01913123.2025.2454691","url":null,"abstract":"<p><p>Breast cancer patients experience more severe emotional distress and depression compared to those with other cancers. Selective serotonin reuptake inhibitors (SSRIs), like citalopram, are commonly used to treat depression. However, the link between SSRI use and breast cancer progression is debated. This study examined the cytotoxic effects of citalopram on triple-negative (MDA-MB231) and ER-positive (MCF-7) breast cancer cells. Results showed a significant decrease in cell viability in both cell lines following citalopram treatment. Interestingly, flow cytometry analysis revealed increased apoptotic cells and induction of cell cycle arrest upon treatment of the cells with citalopram. MCF-7 cells were arrested in the sub-G0-G1 phase, while MDA-MB231 cells accumulated in the S phase. Gene expression analysis demonstrated increased Bax expression and decreased Bcl2 levels. Moreover, cytochrome c and NF-κB were upregulated upon treatment with citalopram. Furthermore, transmission electron microscopy (TEM) analysis of treated cells showed apoptotic morphological changes including shrunken nuclei, membrane blebbing, and chromatin condensation with prominent appearance of autophagosomes and autolysosomes. Additionally, GADD45a and p21, involved in growth arrest and DNA damage, were significantly upregulated. In conclusion, citalopram's ability to induce apoptosis and alter cell cycle suggests its potential in breast cancer treatment.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"158-169"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin II promotes intramural hematoma of aorta in juvenile mice at early stage.","authors":"Weiliang Sun, Changan Yu, Jing Guo, Huina Wang, Shurui Dou, Yuting Zhang, Jingang Zheng, Yanxiang Gao","doi":"10.1080/01913123.2025.2474447","DOIUrl":"10.1080/01913123.2025.2474447","url":null,"abstract":"<p><strong>Objectives: </strong>Intramural hematoma (IMH) is a serious aortic condition characterized by the presence of a contained hematoma within the aortic media. However, the animal model with a high incidence of IMH was lacking, and the specific pathological characteristics of IMH have not been thoroughly characterized.</p><p><strong>Methods and results: </strong>We conducted an experimental study using 4-week-old male, 4-week-old female, and 8-week-old male C57BL/6J mice. These mice were subjected to angiotensin II infusion at a rate of 1000 ng/kg/min for a period of 4 days. In situ imaging was performed, and aorta was harvested and serially sectioned. Histological staining and immunostaining techniques were employed, and the subcellular structure was examined using transmission electron microscopy. Our findings revealed that 4-week-old male mice exhibited a higher susceptibility to angiotensin II-induced IMH, characterized by more circumferential appearances and larger affected areas. Furthermore, IMH was more likely to occur in the upper segment of the descending aorta rather than the lower segment. Within the IMH, older fibrinous thrombus was predominantly observed near the adventitia, while younger red thrombus was more prevalent near the lumen. Additionally, platelet activation and degranulation were observed, along with fibrin cross-linking and thrombus organization, indicating a potential relationship between platelet activation and the progression of IMH.</p><p><strong>Conclusion: </strong>Our study demonstrated that angiotensin II infusion promoted the development of IMH during the early stages, especially in juvenile mice. Furthermore, the presence of platelet activation and thrombus organization suggested their potential involvement in the progression of IMH.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"148-157"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astrocyte alterations during Osmotic Demyelination Syndrome: intermediate filaments, aggresomes, proteasomes, and glycogen storages.","authors":"Jacques Gilloteaux, Corry Charlier, Valérie Suain, Charles Nicaise","doi":"10.1080/01913123.2025.2468700","DOIUrl":"10.1080/01913123.2025.2468700","url":null,"abstract":"<p><strong>Introduction: </strong>A murine model mimicking the human osmotic demyelination syndrome (ODS) revealed with histology demyelinated alterations in the relay posterolateral (VPL) and ventral posteromedial (VPM) thalamic nuclei 12 h and 48 h after chronic hyponatremia due to a fast reinstatement of osmolality. Abnormal expression astrocyte markers ALDHL1 and GFAP with immunohistochemistry in these ODS altered zones, prompted aims to verify in both protoplasmic and fibrillar astrocytes with ultrastructure those changes and other associated subcellular modifications.</p><p><strong>Method: </strong>This ODS investigation included four groups of mice: Sham (NN; <i>n</i> = 13), hyponatremic (HN; <i>n</i> = 11), those sacrificed 12 h after a fast restoration of normal natremia (ODS12h; <i>n</i> = 6), and mice sacrificed 48 h afterward, or ODS48 h (<i>n</i> = 9). Out of those four groups of mice, with LM and ultrastructure microscopy, the thalamic zones included NN (<i>n</i> = 2), HN (<i>n</i> = 2), ODS12h (<i>n</i> = 3) and ODS48h (<i>n</i> = 3) samples. There, comparisons between astrocytes included organelles, GFAP, and glycogen content changes.</p><p><strong>Results: </strong>Thalamic ODS epicenter damages comprised both protoplasmic (PA) and fibrillar (FA) astrocyte necroses along with those of neuropil destructions and neuron Wallerian demyelinated injuries surrounded by a centrifugal region gradient revealing worse to mild destructions. Ultrastructure aspects of resilient HN and ODS12h PAs disclosed altered mitochondria and accumulations of beta- to alpha-glycogen granules that became eventually captured into phagophores as glycophagosomes in ODS48h. HN and ODS12h time lapse FAs accumulated ribonucleoproteins, cytoskeletal aggresomes, and proteasomes but distant and resilient ODS48h FAs maintained GFAP fibrils along with typical mitochondria and dispersed β-glycogen, including in their neuropil surroundings. Thus, ODS triggered astrocyte injuries that involved both post-transcriptional and post-translational modifications such that astrocytes were unable to use glycogen and metabolites due to their own mitochondria defects while accumulated stalled ribonucleoproteins, cytoskeletal aggresomes were associated with proteasomes and GFAP ablation. Resilient but distant astrocytes revealed restitution of amphibolism where typical carbohydrate storages were revealed along with GFAP, as tripartite extensions supply for restored nerve axon initial segments, neural Ranvier's junctions, and oligodendrocyte -neuron junctional contacts.</p><p><strong>Conclusion: </strong>ODS caused astrocyte damage associated with adjacent neuropil destruction that included a regional demyelination caused by a loss of dispatched energetic and metabolic exchanges within the injured region, bearing proportional and collateral centrifugal injuries, which involved reactive repairs time after rebalanced osmolarity.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"170-215"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ultrastructural changes in the adult rat ovary after administration of copper oxide nanoparticles and the possible ameliorative influence of selenium.","authors":"Abeer Mohamed Ali Shalaby, Eman Shaaban Abdel-Aziz Abul-Ela, Amal Mohamed Moustafa, Shehab Hafez Mohamed","doi":"10.1080/01913123.2024.2449091","DOIUrl":"10.1080/01913123.2024.2449091","url":null,"abstract":"<p><p>There is an important concern about the potential health and environmental risks that may develop due to exposure to copper oxide nanoparticles (CuO-NPs). Selenium is an essential trace element. It supports the expression of a variety of selenoproteins. The present study was designed to study the ultrastructural and biochemical changes in the adult rat ovary after oral administration of CuO-NPs and to assess the possible ameliorative influence of Selenium. Sixty adult female albino rats were divided in two major groups: Group I and Group II. Group I was further subdivided into three groups: Group IA (control), Group IB: received a single high dose of 2000 mg/kg CuO-NPs, Group IC: received selenium (0.5 mg/kg), five days before giving a single high dose of CuO-NPs (2000 mg/kg). Thereafter, given selenium for 14 days. Group II was subdivided into three groups: Group IIA (control), Group IIB: received a small dose of 300 mg/kg of CuO-NPs for 28 days, Group IIC: received selenium (0.5 mg/kg), five days before starting concomitant administration of CuO-NPs (300 mg/kg) and Selenium (0.5 mg/kg) for 28 days. Damage of the ovarian ultrastructural features, increased MDA levels, and decreased serum estrogen and progesterone hormones levels were detected in group IB and group IIB. Group IC and group IIC showed improvement of ovarian ultrastructural, decreased MDA levels, and increased serum estrogen and progesterone hormones levels as compared to group IB and group IIB indicating that Selenium could decrease the damage induced by CuO-NPs in the adult rat ovaries.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"109-129"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterozygous missense mutation of the fibrinogen gene associated with cryptogenic liver disease in a 15-months-old Canadian caucasian child.","authors":"Mohit Kehar, Robert J Klaassen, Consolato M Sergi","doi":"10.1080/01913123.2024.2447853","DOIUrl":"10.1080/01913123.2024.2447853","url":null,"abstract":"<p><p>Hepatic fibrinogen storage disease is an uncommon autosomal dominant hereditary illness marked by hypofibrinogenemia and the accumulation of variant fibrinogen in the hepatic endoplasmic reticulum. We present an asymptomatic 15-month-old male with elevated liver enzymes. Test results indicate hypofibrinogenemia. The liver biopsy revealed circular eosinophilic inclusion bodies within the hepatocyte cytoplasm. After diastase pretreatment, the inclusion bodies did not stain using the periodic acid - Schiff procedure. Ultrastructural examination revealed the characteristic fibrinogen storage curvilinear inclusions. Sequence analysis using the Blueprint Genetics (BpG) FLEX Bleeding Disorder/Coagulopathy Panel identified a heterozygous missense variant <i>FGG</i> c.1075 G>C, p. (Gly359Arg). Thus, the patient was diagnosed with hepatic fibrinogen storage disease. Our findings suggest that in patients with asymptomatic elevated liver enzymes presenting with unanticipated hypofibrinogenemia, hepatic fibrinogen storage disorder must be included in the differential diagnosis. Furthermore, our results underscore the significance of molecular diagnosis in patients diagnosed with cryptogenic liver disease.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"235-242"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of copper oxide nanoparticles (CuONPs) on the testes of adult male albino rats and the possible protective role of extra virgin olive oil (EVOO).","authors":"Amany F Mohamed, Safaa M Hanafy, Ranya Mohammed Abdelgalil, Amany M Abo-Ouf","doi":"10.1080/01913123.2025.2462534","DOIUrl":"10.1080/01913123.2025.2462534","url":null,"abstract":"<p><p>We have assessed the effects of copper oxide nanoparticles on the testis of adult male albino rats, and evaluated the protective potential of EVOO, which has antioxidant properties. The study involved treatment of seventy adult male rats followed by examination of their testis. The rats were divided into four groups (I-V), each contained 20 rats except group II which contained 10 rats. Each of groups (I, III, IV) was subdivided equally into two subgroups (A and B). Rats in group I did not receive any treatment (IA) or injected intraperitoneal (IP) with 0.5 ml of distilled water daily for two weeks (IB). Rats in group II were gavaged 0.4 ml EVOO daily for 2 weeks. Rats in group III injected IP daily for 2 weeks with 0.5 ml distilled water containing 1 mg CuO NPs (subgroup IIIA) and 4 mg CuO NPs (IIIB). Rats in group IV were gavaged 0.4 ml EVOO before IP injected daily for 2 weeks with 0.5 ml distilled water containing either 1 mg CuO NPs (subgroup IVA) or 4 mg CuO NPs (IVB). After treatment, morphological, histological and biochemical studies on the testes were conducted. Examination of CuO NPs treated groups revealed dose dependant increase in pathological changes. These changes were reduced body weight, distorted basement membranes of seminiferous tubules and degeneration of seminiferous cells. Co-administration of EVOO ameliorated most pathological changes. We concluded that CuO NPs induced deteriorating changes in rats' testes which were improved after co-administration of EVOO.</p>","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"130-147"},"PeriodicalIF":1.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}