Ultrastructural Pathology最新文献_第5页

Immunohistochemical and ultrastructural identification of telocytes in the infantile hemangioma. 通过免疫组化和超微结构鉴定婴儿血管瘤中的毛细血管细胞。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-10-13 DOI: 10.1080/01913123.2024.2415608

Han-Wen Ding, Qian Wang, Min Wang, Yong Chen, Si-Ming Yuan

{"title":"Immunohistochemical and ultrastructural identification of telocytes in the infantile hemangioma.","authors":"Han-Wen Ding, Qian Wang, Min Wang, Yong Chen, Si-Ming Yuan","doi":"10.1080/01913123.2024.2415608","DOIUrl":"10.1080/01913123.2024.2415608","url":null,"abstract":"Telocytes (TCs) are a distinctive cell entity of the stromal microenvironment of multiple tumors; to date, their existence in infantile hemangioma (IH) remains almost unexplored. This study was therefore undertaken to characterize the immunophenotype, location, morphology, and ultrastructure of telocytes in the IH by means of immunohistochemistry, immunofluorescence confocal microscopy, and transmission electron microscopy. Telocytes were initially identified by CD34, PDGFR-α, Vimentin, and AQP-1 immunostaining. Analyzing the spatial relationship among telocytes, stem cells, endothelial cells, pericytes in the IH with AQP-1/CD31, AQP-1/Glut-1, AQP-1/α-SMA, AQP-1/CD146 and AQP-1/CD133 double immunofluorescence. TCs were immunonegative for CD31, Glut-1, CD146, α-SMA, CD133, and C-kit in the IH. The ultrastructural examination confirmed the presence of TCs, namely stromal cells with characteristic cytoplasmic processes (i.e. telopodes) forming labyrinthine networks around microvessels and releasing extracellular vesicles. Our study provides evidence that telocytes are present and PDGFR-α and AQP-1 are specific antigenic markers in the IH.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"563-574"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The possible effects of chronic administration of amiodarone hydrochloride on the seminiferous tubules of adult male albino rats: histological and biochemical study. 长期服用盐酸胺碘酮对成年雄性白化大鼠曲细精管的可能影响：组织学和生化研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-09-26 DOI: 10.1080/01913123.2024.2407330

Amany M Abo-Ouf, Amany F Mohamed, Heba Abdelnaser Aboelsoud, Ayman Geddawy, Heba F Ibrahim

{"title":"The possible effects of chronic administration of amiodarone hydrochloride on the seminiferous tubules of adult male albino rats: histological and biochemical study.","authors":"Amany M Abo-Ouf, Amany F Mohamed, Heba Abdelnaser Aboelsoud, Ayman Geddawy, Heba F Ibrahim","doi":"10.1080/01913123.2024.2407330","DOIUrl":"10.1080/01913123.2024.2407330","url":null,"abstract":"Amiodarone hydrochloride is an antiarrhythmic agent that is widely prescribed. However, it has serious side effects that approximately affect the whole body organs. In our study, we aimed to assess the possible effects of chronic administration of two different doses of amiodarone hydrochloride on the oxidative and inflammatory parameters as well as the histological morphology and ultrastructure of the seminiferous tubules of adult male albino rats. Forty rats were divided into four groups; Control group 1: each rat did not receive any drugs at all. Control group 2: each rat received 3 ml of 0.16% methylcellulose, orally and daily for 4 weeks. Low dose amiodarone group: each rat received 3 ml of 0.16% methylcellulose contained 3.6 mg amiodarone, orally and daily for 4 weeks. High dose amiodarone group: each rat received 3 ml of 0.16% methylcellulose contained 7.2 mg amiodarone, orally and daily for 4 weeks. Blood samples were collected for measuring serum levels of malondialdehyde, superoxide dismutase, interleukin-6 and tumor necrosis factor-alpha. Testes specimens were examined to assess the morphological changes and the level of expression of caspase-3 apoptotic marker. The results indicated that; amiodarone hydrochloride could induce a dose-dependent toxicity, causing oxidative stress, inflammation, cellular degeneration, deposition of collagen and enhanced apoptosis in the seminiferous tubules.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"476-495"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptozotocin-induced morphological changes in rat lungs. 链脲佐菌素诱导的大鼠肺形态变化

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-11-15 DOI: 10.1080/01913123.2024.2426567

Felicia M Masetla, Mia-Jeanne Van Rooy, June C Serem, Hester M Oberholzer

{"title":"Streptozotocin-induced morphological changes in rat lungs.","authors":"Felicia M Masetla, Mia-Jeanne Van Rooy, June C Serem, Hester M Oberholzer","doi":"10.1080/01913123.2024.2426567","DOIUrl":"10.1080/01913123.2024.2426567","url":null,"abstract":"Streptozotocin (STZ) is a commonly used compound for the induction of type 2 diabetes (T2D) in animal models, but its effects on non-pancreatic tissues like the lungs are not well understood. This study aimed to examine the histopathological impact of STZ on the lungs using male Sprague-Dawley rats. The rats were divided into two groups: a control group on a normal diet and an STZ-treated group receiving a high-fat diet and 10% sucrose water for 8 weeks, followed by an STZ injection (30 mg/kg body weight). All rats were terminated 9 days after STZ administration, and lung samples were collected for light microscopy, transmission electron microscopy (TEM), and confocal laser scanning microscopy. Light microscopy revealed thickening of alveolar septa, narrowing of alveoli, and inflammatory infiltrates in the STZ group. TEM showed mitochondrial damage in type 2 pneumocytes, including membrane fragmentation, cristae loss, and formation of mitochondrial-derived vesicles. Confocal microscopy revealed significantly higher expression of myeloperoxidase, neutrophil elastase, and citrullinated histone 3 in the STZ group compared to controls. These findings suggest that STZ induces considerable lung damage, emphasizing the need to consider lung toxicity in studies involving STZ.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"550-562"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The therapeutic efficacy of adipose mesenchymal stem cell-derived microvesicles versus infliximab in a dextran sodium sulfate induced ulcerative colitis rat model. 脂肪间充质干细胞衍生微囊与英夫利西单抗在右旋糖酐硫酸钠诱导的溃疡性结肠炎大鼠模型中的疗效对比。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-11-15 DOI: 10.1080/01913123.2024.2426566

Sahar F Shaban, Eman A Abdel-Fattah, Manar M Ali, Arigue A Dessouky

{"title":"The therapeutic efficacy of adipose mesenchymal stem cell-derived microvesicles versus infliximab in a dextran sodium sulfate induced ulcerative colitis rat model.","authors":"Sahar F Shaban, Eman A Abdel-Fattah, Manar M Ali, Arigue A Dessouky","doi":"10.1080/01913123.2024.2426566","DOIUrl":"10.1080/01913123.2024.2426566","url":null,"abstract":"Ulcerative colitis (UC) is a chronic relapsing intestinal inflammation that is becoming of increasing incidence worldwide and has insufficient treatment. Therefore, finding effective therapies remains a priority. A dextran sodium sulfate colitis model was established to elucidate colonic layers alterations and compare adipose mesenchymal stem cell-derived microvesicles (MSC-MVs) versus infliximab (IFX) efficacy through biochemical, light, and electron microscope studies. Fifty-four rats were allocated to 4 groups: Control (Con), UC, UC+IFX, and UC+MSC-MVs groups. End body weights (BW) and serum malondialdehyde (MDA) levels were recorded. Colitis severity was estimated by disease activity index (DAI). Colonic specimens were processed to evaluate the histological structure, collagen content, surface mucous and goblet cells, CD44, TNF-α, and GFAP immune expression. Morphometric and statistical analyses were performed. The UC group revealed congested, stenosed colons, a significant decline in end BW, and a significant increase in serum MDA and DAI. Furthermore, disturbed histoarchitecture, inflammatory infiltration, depletion of surface mucous and goblet cells, increased collagen, and TNF-α expression and decreased GFAP expression were observed. Alterations were partially attenuated by IFX therapy, whereas MSC-MVs significantly improved all parameters. In conclusion, MSC-MVs were a superior therapeutic option, via attenuating oxidative stress and inflammatory infiltration, in addition to restoring intestinal epithelial integrity and mucosal barrier.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"526-549"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142640047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of ozone versus mesenchymal stem cell-derived microvesicles in ameliorating testicular changes after hypothyroidism in adult albino rats: a histological and immunohistochemical study. 臭氧与间充质干细胞衍生的微囊在改善成年白化大鼠甲状腺功能减退后睾丸变化方面的功效：组织学和免疫组织化学研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-11-17 DOI: 10.1080/01913123.2024.2423863

Magdy F Gawish, Samia A Abd El-Baset, Salma S Shalabi, Nahla E Ibrahem

{"title":"Efficacy of ozone versus mesenchymal stem cell-derived microvesicles in ameliorating testicular changes after hypothyroidism in adult albino rats: a histological and immunohistochemical study.","authors":"Magdy F Gawish, Samia A Abd El-Baset, Salma S Shalabi, Nahla E Ibrahem","doi":"10.1080/01913123.2024.2423863","DOIUrl":"10.1080/01913123.2024.2423863","url":null,"abstract":"This study was performed to: detect the histological, immunohistochemical, and biochemical alterations that may occur in the testes of adult rats in induced hypothyroidism. And to investigate which one, ozone or MSCs-MVs have better therapeutic effect on testicular changes after hypothyroidism. Eighty-four male adult rats were separated into: control group, hypothyroidism group: rats will be given carbimazole for 30 days, ozone group: rats treated as hypothyroidism group then will be injected with ozone intraperitoneal for 7 days. MSC-MVs group: rats treated as hypothyroidism group then will be injected with a single intravenous dose MSC-MVs. Specimens of testes were handled for light, electron microscope, and immunohistochemical of vimentin and S100. Biochemical analysis for; MDA and TNFα; serum testosterone, TSH, T3, and T4 was done, also, sperm count and morphology assay. Morphometric and statistical analysis were performed. Hypothyroidism group showed disorganized seminiferous tubules. A noticeable gap was between the basement membrane and the germinal epithelium. Wide interstitium had congested vessels and acidophilic homogenous material. Vacuolated germinal epithelium and few germ cells had dark nuclei with noticeable separation of between the basement membrane and the germinal epithelium. Ozone and MSCs-MVs induced improvement in all the previous parameters and restoration of spermatogenesis. In Conclusion MSCs-MVs has better ameliorative effect than ozone on hypothyroidism-exposed testes.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"496-525"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The possible protective effect of ginger extract on toxic changes induced by bisphenol A on the thyroid gland of adult male albino rats: light and electron microscopic study. 生姜提取物对双酚 A 诱导的成年雄性白化大鼠甲状腺毒性变化的可能保护作用：光镜和电子显微镜研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-08-27 DOI: 10.1080/01913123.2024.2395849

Sara Mohammed Taha Denewer, Safinaz Moustafa Mahmoud Ali, Nawal Awad Hasanin, Dalia Refat El-Bassouny

{"title":"The possible protective effect of ginger extract on toxic changes induced by bisphenol A on the thyroid gland of adult male albino rats: light and electron microscopic study.","authors":"Sara Mohammed Taha Denewer, Safinaz Moustafa Mahmoud Ali, Nawal Awad Hasanin, Dalia Refat El-Bassouny","doi":"10.1080/01913123.2024.2395849","DOIUrl":"10.1080/01913123.2024.2395849","url":null,"abstract":"Bisphenol A (BPA) is a chemical substance used in the plastic industry and considered as an endocrine disruptor. Ginger is a herbal material used in the food industry and has antioxidant activity. The present study was performed to evaluate the histological changes in the thyroid gland of adult male albino rats after intake of BPA and if there is any protective role for ginger extract (GE). Eighty adult male rats were divided equally into four groups. Group I as a control group, group II included rats that received 250 mg/kg/day GE orally for eight weeks, group III included rats that received 200 mg/kg/day BPA orally for the same period and group IV included rats that received BPA in the same dose for the same duration concomitantly with GE. At the end of the experiment, blood samples were taken for hormonal essay and tissue samples were processed. Light and electron microscopic studies were done. Morphometric and statistical studies were carried out. Group III showed degenerative changes in the thyroid gland, decreased serum levels of T3 and T4 and a strong positive inducible nitric oxide synthase (iNOS) immune response. Group IV showed restoration of thyroid gland architecture and function. In conclusion, GE protected the thyroid structure from the damaging effect of BPA oxidative stress through its anti-oxidant effect, thus preserving thyroid activity.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"445-475"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Value of electron microscopy as perceived by nephrologists in Flanders, Belgium. 比利时佛兰德斯地区肾病专家对电子显微镜价值的看法。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-11-04 DOI: 10.1080/01913123.2024.2423083

Laura Desnerck, Laure-Anne Kerckhaert, Steven Van Laecke, Amélie Dendooven

{"title":"Value of electron microscopy as perceived by nephrologists in Flanders, Belgium.","authors":"Laura Desnerck, Laure-Anne Kerckhaert, Steven Van Laecke, Amélie Dendooven","doi":"10.1080/01913123.2024.2423083","DOIUrl":"10.1080/01913123.2024.2423083","url":null,"abstract":"Electron microscopy (EM) is an important complementary tool in biopsy diagnosis of kidney disease. However, EM is a costly technique and not universally available. In order to understand nephrologists' perspectives on EM, a survey among Flemish nephrologists was conducted. The survey explores nephrologists' knowledge and satisfaction with EM, the barriers in its use, and its role in decision-making.A questionnaire was sent out to Dutch-speaking nephrologists in Belgium (Flanders) via the professional organization NBVN (Nederlandstalige Belgische Vereniging voor Nefrologie).The average satisfaction of EM accessibility in nephrologists, was 4.0 on a scale from 1 (very unsatisfied) to 5 (very satisfied). The main barrier in ordering EM appeared to be the long turnaround time, indicated by 32.5% of nephrologists. The reports were found mostly understandable by 61.0% of the nephrologists. The impact of EM on diagnosis of kidney disease was estimated higher than its impact on the treatment: 24.4% of respondents estimated diagnosis changes in less than 5% of cases, versus 68.3% estimated treatment changes in less than 5% of cases.This study provides key insights into nephrologists' perception on EM services, revealing high overall satisfaction. However, there is potential for improvement, especially regarding turnaround times.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"575-581"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivation of nucleases with peroxidation damages induced by a menadione: ascorbate combination devastates human prostate carcinomas: ultrastructural aspects. 由甲萘醌：抗坏血酸盐组合诱导的核酸酶再激活与过氧化损伤对人类前列腺癌的破坏：超微结构方面。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI: 10.1080/01913123.2024.2379300

Jacques Gilloteaux, James M Jamison, Jack L Summers, Henryk S Taper

{"title":"Reactivation of nucleases with peroxidation damages induced by a menadione: ascorbate combination devastates human prostate carcinomas: ultrastructural aspects.","authors":"Jacques Gilloteaux, James M Jamison, Jack L Summers, Henryk S Taper","doi":"10.1080/01913123.2024.2379300","DOIUrl":"10.1080/01913123.2024.2379300","url":null,"abstract":"Introduction: Xenografts of androgen-independent human DU145 prostate metastatic carcinomas implanted in nu/nu male mice have revealed a significant survival after a prooxidant anticancer treatment consisting of a combination of menadione bisulfite and sodium ascorbate (VK3:VC).Methods: Implanted samples of diaphragm carcinomas from longest survived mice from either oral, intraperitoneal (IP), or both oral and IP treatment groups were assessed with light, scanning, and transmission electron microscopy to analyze morphologic damages.Results: Compared with previous fine structure data of in vitro untreated carcinomas, the changes induced by oral, IP, and oral with IP VK3:VC treatment dismantled those xenografts with autoschizis, and necrotic atrophy was accomplished by cell's oxidative stress whose injuries were consequent to reactivated deoxyribonucleases and ribonucleases. Tumor destructions resulted from irreversible damages of nucleus components, endoplasmic reticulum, and mitochondria there. Other alterations included those of the cytoskeleton that resulted in characteristic self-excisions named \" autoschizis.\" All these injuries lead resilient cancer cells to necrotic cell death.Conclusion: The fine structure damages caused by VK3:VC prooxidant combination in the human DU145 prostate xenografts confirmed those shown in vitro and of other cell lines with histochemistry and biomolecular investigations. These devastations incurred without damage to normal tissues; thus, our data brought support for the above combination to assist in the treatment of prostate cancers and other cancers.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"378-421"},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of the combination of temozolomide and Eribulin on T98G human glioblastoma cell line: an ultrastructural study. 替莫唑胺和伊瑞布林联合用药对 T98G 人胶质母细胞瘤细胞系的影响：超微结构研究。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-06-25 DOI: 10.1080/01913123.2024.2371821

Gamze Tanriverdi, Belisa Kaleci, Furkan Yavuz, Hakan Sahin, Merjem Purelku, Zeliha Yazici, Sibel Kokturk

{"title":"The effects of the combination of temozolomide and Eribulin on T98G human glioblastoma cell line: an ultrastructural study.","authors":"Gamze Tanriverdi, Belisa Kaleci, Furkan Yavuz, Hakan Sahin, Merjem Purelku, Zeliha Yazici, Sibel Kokturk","doi":"10.1080/01913123.2024.2371821","DOIUrl":"10.1080/01913123.2024.2371821","url":null,"abstract":"Glioblastoma tumors are the most aggressive primary brain tumors that develop resistance to temozolomide (TMZ). Eribulin (ERB) exhibits a unique mechanism of action by inhibiting microtubule dynamics during the G2/M cell cycle phase. We utilized the T98G human glioma cell line to investigate the effects of ERB and TMZ, both individually and in combination. The experimental groups were established as follows: control, E5 (5 nM ERB), T0.75 (0.75 mM TMZ), T1 (1.0 mM TMZ), and combination groups (E5+T0.75 and E5+T1). All groups showed a significant decrease in cell proliferation. Apoptotic markers revealed a time-dependent increase in annexin-V expression, across all treatment groups at the 48-hour time point. Caspase-3, exhibited an increase in the combination treatment groups at the 48-hour mark. Transmission electron microscopy (TEM) revealed normal ultrastructural features in the glioma cells of the control group. However, treatments induced ultrastructural changes within the spheroid glioblastoma model, particularly in the combination groups. These changes included a dose-dependent increase in autophagic vacuoles and apoptotic morphology of the cells. In conclusion, the similarity in the mechanism of action between ERB and TMZ suggests the potential for synergistic effects when combined. Our results highlight that this combination induced severe damage and autophagy in glioma spheroids after 48 hours.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"323-337"},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141447154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential protective effect of propolis on diabetic nephropathy induced by streptozotocin in adult albino rats. 蜂胶对链脲佐菌素诱导的成年白化大鼠糖尿病肾病的潜在保护作用。

IF 1.1 4区医学

Ultrastructural Pathology Pub Date : 2024-09-02 Epub Date: 2024-08-01 DOI: 10.1080/01913123.2024.2386009

Shaimaa M Hafez, Heba F Ibrahim, Shaimaa R Abdelmohsen, Noha A E Yasin, Yara S Abouelela, Heba Abdelnaser Aboelsoud

{"title":"The potential protective effect of propolis on diabetic nephropathy induced by streptozotocin in adult albino rats.","authors":"Shaimaa M Hafez, Heba F Ibrahim, Shaimaa R Abdelmohsen, Noha A E Yasin, Yara S Abouelela, Heba Abdelnaser Aboelsoud","doi":"10.1080/01913123.2024.2386009","DOIUrl":"10.1080/01913123.2024.2386009","url":null,"abstract":"Diabetes mellitus is a common metabolic disorder. It is associated with serious life-threatening complications if not properly managed. The current study aimed at investigating the possible protective role of propolis on streptozotocin-induced diabetic nephropathy. A diabetic rat model was induced by a single intraperitoneal injection of 55 mg/kg streptozotocin. After 4 days, the diabetic rats received oral propolis (300 mg/kg/day) via gastric gavage for 28 days. Biochemical, histopathological and ultrastructural evaluations were performed. The results showed that: streptozotocin-induced diabetes was associated with a marked decrease in the serum high-density lipoproteins and antioxidant enzymes. However, a significant elevation in the levels of serum creatinine, blood urea nitrogen, uric acid, cholesterol, triglycerides and low-density lipoproteins was detected. Furthermore, streptozotocin treatment induced histopathological alterations of the renal cortex; in the form of distorted glomerular capillaries, widened Bowman's space and signs of epithelial tubular degeneration. Ultra-structurally, thickening and irregularity of the glomerular basement membrane and podocytes foot processes effacement were observed. The tubular epithelial cells showed swollen vacuolated mitochondria, scarce basal infoldings and loss of microvilli. Conversely, propolis partially restored the normal lipid profile, antioxidant biomarkers and renal cortical morphology. Propolis exhibited a sort of renoprotection through hypoglycemic, anti-hyperlipidemic and antioxidant effects.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"338-350"},"PeriodicalIF":1.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0