Ultrastructural Pathology最新文献_第6页

Lupus nephritis with cryoglobulinemic glomerulonephritis features: a diagnostic conundrum. 狼疮性肾炎伴冷球蛋白血症肾小球肾炎特征：诊断难题。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-11-30 DOI: 10.1080/01913123.2023.2270030

George Terinte-Balcan, Simona Cinca, Simona Stancu, Mihaela Gherghiceanu, Gabriel Stefan

引用次数: 0

Correction. 修正。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-09-06 DOI: 10.1080/01913123.2023.2248823

引用次数: 0

Morphologically and karyotypically atypical cells of 'normal' human bronchial epithelial cell line (Beas-2B). “正常”人支气管上皮细胞系（Beas-2B）的形态学和核型非典型细胞。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-11-30 DOI: 10.1080/01913123.2023.2262561

Younsu Lee, Young-Joon Ryu

{"title":"Morphologically and karyotypically atypical cells of 'normal' human bronchial epithelial cell line (Beas-2B).","authors":"Younsu Lee, Young-Joon Ryu","doi":"10.1080/01913123.2023.2262561","DOIUrl":"10.1080/01913123.2023.2262561","url":null,"abstract":"Beas-2B is an adenovirus 12-SV40-transfected cell line of \"normal\" human bronchial epithelial cells. This cell line was able to replace normal human bronchial epithelial cells, which are currently unavailable, and served as a model for related studies in numerous toxicology and cancer transformation experiments. In any experiment involving toxins or carcinogens, the basic morphology of Beas-2B should be well characterized prior to exposure, but this has never been properly reported. In this study, atypical cells of the Beas-2B cell line in early passage culture were observed using light and electron microscopy, and the cells were further investigated for abnormal karyotypes by flow cytometry. This Beas-2B cell line could be morphologically categorized into two cell types, A and B. Type A contains a large nucleus and abundant cytoplasm (type A > 95%) and type B contains a small nucleus with dense and scarce cytoplasm (type B < 5%). Both atypical cell types had atypical and multilobed/multinucleated cells, including a high percentage (>30%) of mitotic figures, and were Ki-67 positive (100%). Karyotyping also revealed that 40.4% of the cells had atypical karyotyped chromosomes. In light of these findings, this cell line is no longer a \"normal\" cell, and experiments performed using this cell line can be questioned for non-default results. Experimenters should consider this error in future experiments.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"470-477"},"PeriodicalIF":1.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A closer look: ultrastructural evaluation of high-risk progression IgA nephropathy. 进一步观察:IgA肾病高危进展的超微结构评价。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-11-02 Epub Date: 2023-09-12 DOI: 10.1080/01913123.2023.2256836

George Terinte-Balcan, Gabriel Stefan

{"title":"A closer look: ultrastructural evaluation of high-risk progression IgA nephropathy.","authors":"George Terinte-Balcan, Gabriel Stefan","doi":"10.1080/01913123.2023.2256836","DOIUrl":"10.1080/01913123.2023.2256836","url":null,"abstract":"This retrospective, cross-sectional study sought to examine the ultrastructural characteristics of glomerular lesions using Transmission Electron Microscopy (TEM) in IgA nephropathy (IgAN) and their relationship with the high risk of progression phenotype defined by KDIGO guideline as proteinuria ≥1 g/24 hours despite 3 months of optimized supportive care. We analyzed 81 IgAN patients (median age 41 years, 67% male, eGFR 43.8 mL/min, proteinuria 1.04 g/day); 42 (52%) of them had high risk of progression. There were no differences in terms of age, sex, comorbidities, eGFR, and hematuria between the two groups. High-risk patients more often had segmental glomerulosclerosis (29% vs 8%, p 0.01) in optical microscopy, while in TEM had more frequent podocyte hypertrophy (62% vs 26%, p 0.001) and podocyte foot process detachment from the glomerular basement membrane (19% vs 8%, p 0.05), more often thicker (19% vs 5%, p 0.05) and duplicated (26% vs 10%, p 0.05) glomerular basement membrane, and the presence of subendothelial and subepithelial deposits (31% vs 13%, p 0.05). However, in multivariate binary logistic regression analysis, only podocyte hypertrophy (OR 3.14; 95%CI 1.12, 8.79) was an independent risk factor for high-risk progression in IgAN. These findings highlight the importance of podocytopathy in IgAN progression.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":" ","pages":"461-469"},"PeriodicalIF":1.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10224718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-phospholipase A2 receptor positive membranous nephropathy: investigating the link between electron microscopy stages and clinical outcome. 抗磷脂酶A2受体阳性膜性肾病:探讨电镜分期与临床预后之间的联系。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-09-03 DOI: 10.1080/01913123.2023.2236225

Gabriel Stefan, George Terinte Balcan, Nicoleta Petre, Simona Cinca, Adrian Zugravu, Simona Stancu

{"title":"Anti-phospholipase A2 receptor positive membranous nephropathy: investigating the link between electron microscopy stages and clinical outcome.","authors":"Gabriel Stefan, George Terinte Balcan, Nicoleta Petre, Simona Cinca, Adrian Zugravu, Simona Stancu","doi":"10.1080/01913123.2023.2236225","DOIUrl":"https://doi.org/10.1080/01913123.2023.2236225","url":null,"abstract":"This retrospective, observational study sought to examine the relationship between Ehrenreich-Churg electron microscopy (EM) stages and long-term outcomes in anti-PLA2R membranous nephropathy (MN). Seventy-one patients with anti-PLA2R MN (median titer 185.7RU/mL) were followed for a median of 46 months, with end-stage kidney disease (ESKD) as the primary endpoint, and response to treatment as a secondary endpoint. Patients were grouped into stages I-II (41 patients) and stages III-IV (30 patients) for analytical purposes. Notably, the III-IV group demonstrated a lower eGFR, lower anti-PLA2R titer, but a higher chronicity score. Kaplan-Meier analysis showed shorter mean kidney survival time in stages III-IV compared to I-II (p 0.03). However, multivariate analysis using Cox regression indicated that Ehrenreich-Churg stages did not significantly influence kidney survival, but lower eGFR at diagnosis and higher histopathological chronicity score did. Remission was achieved by 64% of patients and no relationship between Ehrenreich-Churg stages and treatment response was found. The only identified risk factor for not achieving remission was the severity of hyposerinemia at diagnosis. In conclusion, while EM stages III-IV are associated with more chronic lesions and stages I-II with more active immunologic disease, the histological chronicity score seems to be a stronger predictor of long-term outcomes.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 5","pages":"365-372"},"PeriodicalIF":1.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10101967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effects of cisplatin and jaceosidin on SH-SY5Y neuroblastoma cells: an electron microscopic, molecular and biochemical study. 顺铂和紫叶黄素对SH-SY5Y神经母细胞瘤细胞的影响:电镜、分子和生化研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-09-03 DOI: 10.1080/01913123.2023.2218911

Pinar Bayram, Selina Aksak Karamese, Bengul Özdemir, Aysegul Durak, Deniz Billur

{"title":"The effects of cisplatin and jaceosidin on SH-SY5Y neuroblastoma cells: an electron microscopic, molecular and biochemical study.","authors":"Pinar Bayram, Selina Aksak Karamese, Bengul Özdemir, Aysegul Durak, Deniz Billur","doi":"10.1080/01913123.2023.2218911","DOIUrl":"https://doi.org/10.1080/01913123.2023.2218911","url":null,"abstract":"In this study, our aim was to show both the single and combined effects of cisplatin and jaceosidin in SHSY-5Y neuroblastoma cells. For this purpose, we used MTT cellular viability assay, Enzyme-Linked Immunosorbent Assay (ELISA), Transmission Electron Microscopy (TEM), Immunofluorescence Staining Assay (IFA) and Western blotting (WB) assay. According to MTT findings, IC50 dose was detected as 50 µM cisplatin and 160 µM jaceosidin co-application. Therefore, experimental groups were finally selected as control, cisplatin, 160 µM jaceosidin and Cisplatin +160 µM jaceosidin. Cell viability was decreased in all groups, and the IFA findings confirmed the viability analysis. WB data indicated that matrix metalloproteinase 2 and 9 levels, as indicators of metastasis, decreased. While LPO and CAT levels increased in all treatment groups, it was observed that the activity of SOD decreased. When TEM micrographs were investigated, cellular damages were determined. In the light of these results, it can be said that cisplatin and jaceosidin have a potential to increase the effects of each other synergistically.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 5","pages":"388-397"},"PeriodicalIF":1.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10154299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of busulfan on the testicular structure in prepubertal rats: A histological, ultrastructural and immunohistochemical study. 丁硫丹对青春期前大鼠睾丸结构的影响:组织学、超微结构和免疫组织化学研究。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-09-03 DOI: 10.1080/01913123.2023.2234470

Reem Ibrahim Abd El-Hay, Walaa H E Hamed, Nesreen Mostafa Omar, Dalia Refat El-Bassouny, Salwa A Gawish

{"title":"The impact of busulfan on the testicular structure in prepubertal rats: A histological, ultrastructural and immunohistochemical study.","authors":"Reem Ibrahim Abd El-Hay, Walaa H E Hamed, Nesreen Mostafa Omar, Dalia Refat El-Bassouny, Salwa A Gawish","doi":"10.1080/01913123.2023.2234470","DOIUrl":"https://doi.org/10.1080/01913123.2023.2234470","url":null,"abstract":"Busulfan is a widely used cancer chemotherapeutic agent. Temporary or permanent sterility in male patients is one of the most common side effects of this drug. The present study was performed to evaluate the changes in the microscopic structure of the testes of prepubertal rats, as well as the changes in PCNA and caspase-3 immune expression, at different durations after busulfan administration. The rats were 5 weeks old and were divided into two main groups. Control group and busulfan treated group. Busulfan treated group received a single dose of busulfan (40 mg/kg), then animals were subdivided to three subgroups; IIa, IIb, IIc which were sacrificed after four, ten and twenty weeks, respectively, from the beginning of the experiment. Light and electron microscopic studies were done. Serum testosterone level and relative testes weight were assessed. Immunohistochemical staining for anti-proliferating cell nuclear antigen (PCNA) and anti-caspase-3 antigen was also done. Morphometric and statistical studies were carried out. Group II revealed histological and ultrastructural degenerative changes including congested blood vessels and degenerated spermatogenic epithelium, Sertoli cells, and Leydig cells. These changes were more evident after 10 weeks of busulfan administration and were accompanied by absence of mature sperms in the lumen of seminiferous tubules. These changes were associated with a significant reduction in relative testes weight, testosterone level, germinal epithelial height and seminiferous tubule diameter. Moreover, PCNA and caspase-3 immune expression was significantly altered in busulfan treated group. Mild improvement in testicular structure was observed 20 weeks after busulfan treatment.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 5","pages":"424-450"},"PeriodicalIF":1.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10098811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung cancer patient with Tropheryma whipplei and Nocardia co-infection. 肺癌伴鞭瘤和诺卡菌合并感染的患者。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-09-03 DOI: 10.1080/01913123.2023.2241888

Yuli Zhou, Nan Yang, Senlin Ruan, Shenghai Wu, Daojun Yu, Juan Jin

{"title":"Lung cancer patient with Tropheryma whipplei and Nocardia co-infection.","authors":"Yuli Zhou, Nan Yang, Senlin Ruan, Shenghai Wu, Daojun Yu, Juan Jin","doi":"10.1080/01913123.2023.2241888","DOIUrl":"https://doi.org/10.1080/01913123.2023.2241888","url":null,"abstract":"A 34-year-old male presented with lung shadow and was asymptomatic during medical examination. The patient had a prior history of thyroid tumors. Imaging manifestation showed a nodule in the medial segment of the right middle lobe, with partial obstruction of the distal bronchus within the lesion. Ground-glass and inflammatory nodules were observed in the anterior segment of the right upper lobe, as well as chronic inflammatory changes in the lower lobe of the right lung. Lung histopathological examination suggested invasive adenocarcinoma. A morphological examination of the bronchoalveolar lavage fluid revealed the presence of Tropheryma whipplei (TW) and Nocardia. Although TW infection has been reported in cancer patients, co-infection with Nocardia is a unique occurrence in this case. Opportunistic pathogens are common in immunocompromised patients but in this case, the patient was a young adult with normal immunity and an early-stage tumor with TW and Nocardia co-infection. We demonstrated the presence of rare microorganisms through imaging findings, combined with different staining methods of bronchoalveolar lavage fluid and lung tissue sections and evaluation of morphological characteristics. The aim of the present study was to provide early diagnosis and treatment of patients by improving microbial morphological detection.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 5","pages":"451-459"},"PeriodicalIF":1.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10102465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cilia impairment in bronchial epithelial cells detected in autopsy material of SARS-CoV-2-infected patient. sars - cov -2患者尸检材料中支气管上皮细胞纤毛损伤的检测

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-09-03 DOI: 10.1080/01913123.2023.2222167

Daria M Potashnikova, Tatiana N Sotnikova, Olesya M Shirokova, Oleg V Zayratyants, Elena Yu Vasilieva, Eugene V Sheval

引用次数: 0

Podocytopathies related to either COVID-19 infection or its vaccination, our experience and literature review. 与COVID-19感染或疫苗接种相关的足细胞病变，我们的经验和文献综述。

IF 1 4区医学

Ultrastructural Pathology Pub Date : 2023-09-03 DOI: 10.1080/01913123.2023.2237565

Ashbita Pokharel, Jessica D Anderson, Mustafa Deebajah, Neal B Blatt, Gampala Reddy, Vamshi Garlapaty, Wei Li, Hassan D Kanaan, Ping L Zhang

{"title":"Podocytopathies related to either COVID-19 infection or its vaccination, our experience and literature review.","authors":"Ashbita Pokharel, Jessica D Anderson, Mustafa Deebajah, Neal B Blatt, Gampala Reddy, Vamshi Garlapaty, Wei Li, Hassan D Kanaan, Ping L Zhang","doi":"10.1080/01913123.2023.2237565","DOIUrl":"https://doi.org/10.1080/01913123.2023.2237565","url":null,"abstract":"Coronavirus disease 2019 (COVID-19) affects several organs including the kidney resulting in acute kidney injury (AKI) and variants of podocytopathies. From the beginning to the middle period of the COVID-19 pandemic, we have collected eight renal biopsies with various renal diseases including 4 podocytopathies. In addition, from the middle period to the near end of the COVID-19 pandemic, we have seen two of the patients who developed nephrotic syndrome following COVID-19 vaccination. Three of 4 podocytopathies were collapsing glomerulopathy (also called collapsing focal segmental glomerulosclerosis) and the fourth was a minimal change disease (MCD). Two of three collapsing glomerulopathy were found in African American patients, one of who was tested positive for having the high-risk allele APOL-1 G1. In addition, the two renal biopsies showed either MCD or replaced MCD following COVID-19 vaccination. MCD can be a rare complication following COVID-19 infection and COVID-19 vaccination, raising the question if there are similar antigens induced by the infection or by the vaccination that trigger the MCD. This article reports our experience of diagnosing podocytopathies related to either COVID-19 infection or its vaccination and provides a literature review regarding the incidence and potential pathophysiology in the field.","PeriodicalId":23430,"journal":{"name":"Ultrastructural Pathology","volume":"47 5","pages":"373-381"},"PeriodicalIF":1.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1