Therapeutic potential of mesenchymal stem cells-derived exosomes on experimentally induced neurodegeneration in olfactory bulb of adult male albino rat: histological, physiological and biochemical studies.

IF 1.2 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2026-01-01 Epub Date: 2026-01-18 DOI:10.1080/01913123.2026.2616037

Mayada M Elkeblawy, Mona Tayssir Sadek, Azza A M Abouraia, Eman M El-Beltagi

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Abstract

Neurodegeneration (ND) is a major medical problem in elderly. Olfactory bulb represents an early-vulnerable brain area to ND. Mesenchymal stem cells-derived exosomes are currently evaluated as a possible therapeutic agent for several diseases. This study aimed to assess exosomes-therapeutic potential on ND-induced olfactory bulb changes. Forty-five adult male albino rats were equally categorized into three groups: control (I), ND (II), and exosomes treated (III). ND was induced in groups II and III using aluminum chloride, then group III rats received a single intravenous injection of 200 µg exosomes. Buried food test was conducted to assess sense of olfaction. Blood was collected to assess serum malondialdehyde (MDA) and total antioxidant capacity (TAC) levels. Olfactory bulb specimens were prepared for histological and immunohistochemical (GFAP & synaptophysin) studies. ND group displayed a highly significant increase in buried food test time and MDA level, along with decrease in TAC level. Marked histological changes were observed affecting nerve cells, synapses, neuropil and bulb vasculature with a highly significant increase in GFAP area percentage and decrease in that of synaptophysin. Treatment with exosomes in group III markedly ameliorated these changes. These results reflected that MSCs-derived exosomes had a remarkable therapeutic potential against ND-induced changes in olfactory bulb.

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间充质干细胞来源的外泌体对成年雄性白化大鼠嗅球神经变性的治疗潜力：组织学、生理和生化研究。

神经退行性变（ND）是老年人的主要医学问题。嗅球代表了早期易受ND影响的大脑区域。间充质干细胞衍生的外泌体目前被评估为几种疾病的可能治疗剂。本研究旨在评估外泌体对nd诱导的嗅球变化的治疗潜力。45只成年雄性白化大鼠平均分为三组：对照组(I), ND （II）和外泌体治疗组（III）。II、III组大鼠采用氯化铝诱导ND， III组大鼠单次静脉注射外泌体200µg。用埋藏食物试验评价嗅觉。采集血液评估血清丙二醛（MDA）和总抗氧化能力（TAC）水平。制备嗅球标本进行组织学和免疫组织化学（GFAP & synaptophysin）研究。ND组大鼠埋食试验时间和MDA水平显著增加，TAC水平显著降低。神经细胞、突触、神经节和球茎血管发生明显组织学改变，GFAP面积百分比显著升高，突触素面积百分比显著降低。III组外泌体治疗显著改善了这些变化。这些结果表明，骨髓间充质干细胞衍生的外泌体对nd诱导的嗅球改变具有显著的治疗潜力。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.