Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study.

IF 1.1 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2025-01-01 Epub Date: 2025-04-17 DOI:10.1080/01913123.2025.2494618

Mehmet Berker, Sibel Köktürk, Sibel Doğan, Emel Usta

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Abstract

The development of new strategies to raise the survival and viability of transplanted mesenchymal stem cells (MSCs) is very important for the therapeutic potential of stem cells. The natural flavonoid myricetin has anticancer, antioxidant, anti-inflammatory and antiapoptotic effects. The effects of myricetin on human umbilical cord-derived MSCs (HUC-MSCs) induced oxidative stress with hydrogen peroxide (H₂O₂) were evaluated by transmission electron microscopy (TEM) and immunocytochemistry (ICC) staining. Myricetin showed an increase in the number of live cells, a decrease in caspase-3 and tumor necrosis factor-α (TNF-α) ICC staining intensity, an increase in the translocase of the mitochondrial inner membrane 17 (TIM17) ICC staining intensity, and a decrease in degeneration of cell ultrastructure in TEM against oxidative stress damage in HUC-MSCs. The results suggest that myricetin prevents oxidative stress-induced apoptosis and inflammation in the HUC-MSCs. Myricetin can be combined with HUC-MSCs in cell culture and considered as a supportive alternative treatment option.

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杨梅素改善过氧化氢诱导的人间充质干细胞氧化应激的影响：一项超微结构和免疫细胞化学研究。

提高移植间充质干细胞（MSCs）的存活率和活力的新策略的发展对干细胞的治疗潜力非常重要。天然黄酮类杨梅素具有抗癌、抗氧化、抗炎、抗细胞凋亡等作用。通过透射电镜（TEM）和免疫细胞化学（ICC）染色观察杨梅素对过氧化氢（H2O2）诱导的人脐带源性间充质干细胞（HUC-MSCs）氧化应激的影响。杨梅素使HUC-MSCs的活细胞数量增加，caspase-3和肿瘤坏死因子-α (TNF-α) ICC染色强度降低，线粒体内膜转位酶17 (TIM17) ICC染色强度增加，TEM氧化应激损伤时细胞超微结构变性降低。结果提示杨梅素可抑制氧化应激诱导的HUC-MSCs的凋亡和炎症。杨梅素可以在细胞培养中与HUC-MSCs联合使用，并被认为是一种支持性的替代治疗选择。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.