辐照骨和骨坏死骨的微观结构变化:扫描电镜研究。

IF 1.1 4区 医学 Q4 MICROSCOPY
Ultrastructural Pathology Pub Date : 2024-03-03 Epub Date: 2024-02-07 DOI:10.1080/01913123.2023.2295458
P Sridhar Reddy, Kaapo Villikka, Bina Kashyap, Hannah Dekker, Engelbert A J M Schulten, Jopi J W Mikkonen, Mikael Turunen, Arto P Koistinen, Nathalie Bravenboer, Arja M Kullaa
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引用次数: 0

摘要

由于包括下颌骨在内的骨骼受到放射线照射,对骨代谢和愈合造成有害影响,感染和骨坏死的风险也随之增加,因此放射线照射是一个主要的健康问题。本研究旨在通过扫描电子显微镜(SEM)研究放疗引起的人类下颌骨微结构变化。下颌骨皮质骨活组织取自对照组、照射组和骨软化症(ORN)患者。制备的骨样本用于光学显微镜和扫描电子显微镜检查。扫描电子显微镜图像分析了骨小梁数量、哈弗氏管(HC)数量、骨小梁直径(D.O)、哈弗氏管直径(D.HC)、骨壁厚度(O.W.Th)、骨细胞数量和骨细胞树突数量。与对照组相比,辐照组和 ORN 组的骨小梁数量、D.O、D.HC、O.W.Th、骨细胞数量和骨细胞树突数量均显著减少(p 5 年平均辐射活检间隔)。骨的潜在微观损伤增加了其易感性,并进一步导致辐射诱导的骨变化甚至 ORN。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microstructural changes in the irradiated and osteoradionecrotic bone: a SEM study.

Radiation exposure is a major health concern due to bone involvement including mandible, causing deleterious effects on bone metabolism, and healing with an increasing risk of infection and osteoradionecrosis. This study aims to investigate the radiotherapy-induced microstructural changes in the human mandible by scanning electron microscopy (SEM). Mandibular cortical bone biopsies were obtained from control, irradiated, and patients with osteoradionecrosis (ORN). Bone samples were prepared for light microscopy and SEM. The SEM images were analyzed for the number of osteons, number of Haversian canal (HC), diameter of osteon (D.O), the diameter of HC (D.HC), osteonal wall thickness (O.W.Th), number of osteocytes, and number of osteocytic dendrites. The number of osteons, D.O, D.HC, O.W.Th, the number of osteocytes, and osteocytic dendrites were significantly decreased in both irradiated and ORN compared to controls (p < .05). The number of HCs decreased in irradiated and ORN bone compared to the control group. However, this was statistically not significant. The deleterious effect of radiation continues gradually altering the bone quality, structure, cellularity, and vascularity in the long term (>5 years mean radiation biopsy interval). The underlying microscopic damage in bone increases its susceptibility and contributes further to radiation-induced bone changes or even ORN.

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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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