COVID-19患者心肌内皮细胞胞内细胞器重构:一项基于尸检的研究

IF 1.1 4区 医学 Q4 MICROSCOPY
Ultrastructural Pathology Pub Date : 2024-01-02 Epub Date: 2024-01-09 DOI:10.1080/01913123.2023.2286977
Nataliya Bgatova, Sergey Savchenko, Alexei Lamanov, Iuliia Taskaeva, Boris Ayzikovich, Valentina Gritcinger, Andrey Letyagin, Maksim Korolev
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引用次数: 0

摘要

已知,感染SARS-CoV-2的患者的不良结果可能与病毒直接作用引起的心脏损伤并发症的发生有关。这些由SARS-CoV-2感染引起的心血管损伤机制尚不完全清楚;然而,对covid -19相关心肌微循环功能障碍的研究可以代表解决这一挑战的有用策略。因此,我们旨在研究COVID-19患者心肌毛细血管内皮细胞的超微结构组织。用透射电镜观察新冠肺炎患者心肌毛细血管内皮细胞形态。新冠肺炎患者心肌毛细血管内皮细胞具有丰富的粗内质网(ER)膜、高尔基复合物、游离核糖体和脂质多体复合物等特征。内皮细胞细胞质中存在双膜囊泡,内含病毒粒子和拉链内质网。内皮细胞超微结构的改变提示了SARS-CoV-2感染期间细胞膜的重塑。我们的发现证实了心肌内皮细胞中病毒诱导结构的形成被认为是病毒复制和组装的关键。这些数据可能阐明COVID-19患者内皮功能障碍发展的机制,为药物治疗提供潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intracellular organelles remodeling in myocardial endotheliocytes in COVID-19: an autopsy-based study.

It is known that the unfavorable outcome in patients infected with SARS-CoV-2 may be associated with the development of complications caused by heart damage due to the direct virus action. The mechanism of these cardiovascular injuries caused by SARS-CoV-2 infection has not been fully understood; however, the study of COVID-19-associated myocardial microcirculatory dysfunction can represent the useful strategy to solving this challenge. Thus, here we aimed to study the ultrastructural organization of endothelial cells of myocardial capillaries in patients with COVID-19. The morphology of endotheliocytes of the myocardial blood capillaries in patients with COVID-19 was studied on cardiac autopsy material using transmission electron microscopy. The endotheliocytes of myocardial capillaries in patients with COVID-19 were characterized by the abundant rough endoplasmic reticulum (ER) membranes, the Golgi complex, and free polysomal complexes of ribosomes and lipids. The presence of double membrane vesicles with virions and zippered ER was detected in the cytoplasm of endotheliocytes. The revealed endothelial ultrastructural changes indicate the remodeling of intracellular membranes during SARS-CoV-2 infection. Our findings confirm the formation of virus-induced structures in myocardial endothelial cells considered critical for viral replication and assembly. The data may elucidate the mechanisms of endothelial dysfunction development in patients with COVID-19 to provide potential targets for drug therapy.

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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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