Turkish Journal of Medical Sciences最新文献

{"title":"Relationship between hand osteoarthritis severity and serum resistin levels in women with metabolic syndrome: a case-control study.","authors":"Ekin Başak Doğanci, Berna İmge Aydoğan, Nilgün Başkal, Safiye Tuncer","doi":"10.55730/1300-0144.5964","DOIUrl":"10.55730/1300-0144.5964","url":null,"abstract":"<p><strong>Background/aim: </strong>The aim of this study was to determine the relationship between clinical and radiological hand osteoarthritis (OA) severity and serum resistin levels in women diagnosed with metabolic syndrome (MetS).</p><p><strong>Materials and methods: </strong>This research was designed as a case-control study. A total of 87 women, aged 50-65 years, were evaluated between January to March 2014, comprising 29 patients diagnosed with MetS and hand OA (MetS (+), hand OA (+); group 1), 29 patients without MetS but with hand OA (MetS (-), hand OA (+); group 2), and 29 healthy controls (MetS (-), hand OA (-); group 3). The diagnostic criteria of the American College of Rheumatology (ACR) for hand OA and National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) for MetS were used. The presence and severity of pain were evaluated according to the Likert scale, function by measuring grip and pinch strength, the presence of disability by Duruöz hand score (DHS), and structural damage by the Osteoarthritis Research Society International (OARSI) atlas classification.</p><p><strong>Results: </strong>There were no significant differences between the three groups in terms of the presence and severity of pain, functional assessment parameters, disability scores, and serum resistin levels. Structural damage was more severe in group 2. No significant association was found between the serum resistin levels and pain severity, functional assessment parameters, disability scores, hand OA findings (total number of tender joints, the total number of nodules, etc.), and structural damage.</p><p><strong>Conclusion: </strong>MetS did not contribute to clinical and radiological hand OA severity. Serum resistin levels were not higher in patients with MetS and had no effect on hand OA severity.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"55 1","pages":"242-249"},"PeriodicalIF":1.2,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nationwide study on the prevalence of rheumatoid factor and anticitrullinated peptide positivity and their contribution to rheumatoid arthritis diagnosis.","authors":"Hasan Satiş, Abdulsamet Erden, Emre Bilgin, Gizem Ayan, Berkan Armağan, Duygu Tecer, Alper Sari, Umut Kalyoncu, Murat Çağlayan, Mustafa Mahir Ülgü, Mustafa Okan Ayvali, Naim Ata, Şuayip Birinci","doi":"10.55730/1300-0144.5872","DOIUrl":"10.55730/1300-0144.5872","url":null,"abstract":"<p><strong>Background/aim: </strong>Rheumatoid factor (RF) and anticitrullinated peptide (anti-CCP) are specific for rheumatoid arthritis (RA) diagnosis. However, they could be positive in other diseases and even in healthy populations. The aim was to investigate the prevalence of positive RF and anti-CCP antibodies in persons admitted to hospital for any reason and on a national scale.</p><p><strong>Materials and methods: </strong>The National Electronic Health Database, which contains the clinical records of over 80 million people, was used to design this multicenter, retrospective cohort study. The subjects included in the study were divided into age groups according to 10-year periods. RA cases were identified using ICD-10 codes that included M05, M06, M08, and their subgroups. RF and anti-CCP positivity were evaluated in terms of their contribution to the risk of being diagnosed with RA, with the change according to age and sex.</p><p><strong>Results: </strong>During the 1.1.2018-31.12.2021 period, 13,918,072 RF tests were performed in 11,849,440 people, whereas 1,183,607 anti-CCP tests were performed in 1,020,967 people. Moreover, 797,089 people had both tests performed at least once. The RF positivity rate in patients who only requested RF tests was 14.72% and it was 35.04% for anti-CCP positivity in those who only requested anti-CCP tests. The rate of concomitant RF and anti-CCP positivity was 22.56%. An RA diagnosis was made in 27.8% of RF-positive people, 39.73% of anti-CCP-positive people, and 56.6% of co-RF and anti-CCP-positive people. RF positivity and concomitant RF and anti-CCP positivity increased with age and were more common in females.</p><p><strong>Conclusion: </strong>RF and anti-CCP positivity may be seen in a healthy population with female predominance. As age increases, the risk of RF positivity rises, but anti-CCP positivity does not change. Concomitant RF and anti-CCP positivity shows the highest risk of RA development with respect to either antibody positivity alone.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 5","pages":"949-955"},"PeriodicalIF":1.2,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new scoring in differential diagnosis: multisystem inflammatory syndrome or adenovirus infection?","authors":"Mustafa Gençeli, Talha Üstüntaş, Özge Metin Akcan, Sinan Saylik, Fatih Ercan, Sevgi Pekcan, Sipil Gençeli, Sevgi Yaşar Durmuş, Mustafa Argun","doi":"10.55730/1300-0144.5905","DOIUrl":"10.55730/1300-0144.5905","url":null,"abstract":"<p><strong>Background/aim: </strong>Differentiating multisystem inflammatory syndrome in children (MIS-C) from adenovirus infection (AI) can be challenging due to similar clinical and laboratory findings. This study aimed to identify distinguishing characteristics and develop a scoring system to facilitate accurate diagnosis.</p><p><strong>Materials and methods: </strong>A comprehensive review of medical records was undertaken for 108 MIS-C patients and 259 patients with confirmed AI. A comparison of laboratory data and clinical findings was conducted across the patient groups using appropriate statistical tests.</p><p><strong>Results: </strong>The MIS-C patients were significantly older than the AI patients (p < 0.001). Diarrhea, rash, abdominal pain, vomiting, nonexudative conjunctivitis, lymphadenopathy tachycardia, bradycardia, hypotension, hypoxia seizures, agitation, headache, and altered consciousness symptoms were more frequently associated with MIS-C (p < 0.001), while cough and runny nose were significantly more common in AI (p < 0.001). Lymphopenia and thrombocytopenia were more prevalent in the MIS-C patients (p < 0.001). AI and MIS-C were scored by giving one point each to the parameters that created the difference. For AI, being ≤60 months of age, the presence of cough, runny nose and absence of diarrhea, rash, abdominal pain, vomiting, nonexudative conjunctivitis, lymphadenopathy, tachycardia, bradycardia, hypotension, hypoxia seizures, agitation, headache, and altered consciousness, lymphopenia, thrombocytopenia, and C-reactive protein value <124.5 mg/L were determined as each parameter plus one point. A total score above 14 could predict AI with a high degree of accuracy, with sensitivity at around 97.5% and specificity at 92.6%.</p><p><strong>Conclusion: </strong>The proposed inpatient scoring system, when used in conjunction with polymerase chain reaction testing, may improve the early differentiation of AI and MIS-C. This approach could help reduce unnecessary testing and optimize resource allocation. Further research with larger samples should investigate this novel scoring system to establish its reliability and generalizability.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 6","pages":"1237-1243"},"PeriodicalIF":1.2,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid and cardiovascular diseases.","authors":"Sinem Başak Tan Öksüz, Mustafa Şahin","doi":"10.55730/1300-0144.5927","DOIUrl":"10.55730/1300-0144.5927","url":null,"abstract":"<p><p>The thyroid gland is one of the major regulator organs of hemostasis in the human body, controlling the functioning of numerous systems. Thyroid hormones exert a modulating effect on the cardiovascular system in particular, ensuring optimal functioning within the normal range. Triiodothyronine (T3), as an active form of thyroid hormone, is mainly responsible for this effect via both genomic and nongenomic mechanisms. It has been reported that overt thyroid disorders are associated with a number of cardiovascular diseases and cardiac mortality. While hyperthyroidism appears to be related to atrial fibrillation and heart failure, the most pronounced cardiovascular complication of hypothyroidism seems to be atherosclerosis. Achieving euthyroidism is of great importance for restoring cardiovascular function. However, depending on the underlying health conditions, this may not be possible for all patients. Furthermore, there has been a growing focus on the role of subclinical thyroid dysfunctions and their impacts on the cardiovascular system in recent years. The pattern of cardiovascular abnormalities in subclinical thyroid disorders appears to parallel that of overt hypothyroidism, suggesting that even mild alterations in thyroid hormone levels may also have effects on the cardiovascular system. The management of subclinical thyroid disease remains controversial. Current evidence suggests that patient age and underlying cardiovascular diseases are major factors in clinical decision-making.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 7","pages":"1420-1427"},"PeriodicalIF":1.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"A retrospective study on the clinical and molecular outcomes of calpainopathy in a Turkish patient cohort\": [Turkish Journal of Medical Sciences 54 (1) 2024 86-98].","authors":"İzem Olcay Şahin, Emine Karataş, Mikail Demir, Büşra Tan, Hüseyin Per, Yusuf Özkul, Munis Dündar","doi":"10.55730/1300-0144.5861","DOIUrl":"10.55730/1300-0144.5861","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.55730/1300-0144.5769.].</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 4","pages":"880"},"PeriodicalIF":1.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11407355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico study of bioactive compounds derived from Indonesian marine invertebrates as a novel antituberculosis agent.","authors":"Ayu Masyita, Eris Septiana, Asep Bayu, Bustanussalam Bustanussalam, Jonathan A Panggabean, Firdayani Firdayani, Tutik Murniasih","doi":"10.55730/1300-0144.5923","DOIUrl":"10.55730/1300-0144.5923","url":null,"abstract":"<p><strong>Background/aim: </strong>Tuberculosis (TB) has become the world's deadliest disease. The lack of an effective therapeutic drug to treat it is one of the obstacle for doctors. Today, multidrug-resistant TB cases are increasing. Investigating these new drug should be given intensive and careful consideration. Marine invertebrates are valuable since they produce a large number of active compounds, and screening of these active compounds is very important.</p><p><strong>Materials and methods: </strong>Anti-TB screening of compounds derived from marine invertebrates was performed via the in silico method. Three-dimensional structures of pantothenate kinase (MtPanK type 1, PDB ID: 4BFT), <i>Mycobacterium tuberculosis</i> InhA (PDB ID: 2X23), protein kinase B (PDB ID: 5U94), and β-ketoacyl acyl carrier protein synthase I (MtKasA, PDB ID: 2WGE) were used as the protein targeted receptors.</p><p><strong>Results: </strong>The molecular docking analysis showed that the potential candidate compounds with the lowest docking score were 19-hydroxypsammaplysin Q, 19-hydroxypsammaplysin S, psammaplysin L, and psammaplysin K dimethoxy acetal. Several compounds, such as molamide C and the manzamine group, are also potential anti-TB compounds.</p><p><strong>Conclusion: </strong>This study showed that psammaplysin groups have potential as anti-TB compounds. Further laboratory experiments should be done to confirm the in silico data.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 6","pages":"1399-1408"},"PeriodicalIF":1.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with morbidity of a totally implantable venous access device in patients with breast cancer.","authors":"Burak Ilhan, Berkay Kiliç","doi":"10.55730/1300-0144.5904","DOIUrl":"10.55730/1300-0144.5904","url":null,"abstract":"<p><strong>Background/aim: </strong>To investigate the possible morbidities associated with the implantation of a totally implantable venous access device (TIVAD) in breast cancer (BC) patients.</p><p><strong>Materials and methods: </strong>Clinical data and developed complications in 546 BC patients with TIVAD between 2017 and 2021 were analyzed retrospectively. Among these, 524 (96%) patients who underwent TIVAD implantation via the right subclavian vein (SCV) route were examined separately.</p><p><strong>Results: </strong>The mean patient age was 56.1 ± 11.4 years, and the mean patient body mass index was 25.3 ± 6.5. The incidence of early complications was 2.3% (12 cases), and the late complication incident rate was 2.6% (14 cases). Among these complications, ten (1.9%) catheter-related infections developed more frequently than others. Early complications increased as the number of puncture attempts rose and decreased with ultrasound guidance in the implantation, but no other predictive factors increased. No correlation existed between the patients' age, body mass index, BC side, puncture method, puncture number, surgical approach, radiotherapy, and late complications. There was no evidence that postoperative effects specific to BC surgery, such as increased pain, decreased functional capacity, and lymphedema, were altered with TIVAD implantation. For catheter-related infections only, adjuvant radiotherapy was a risk factor for patients with various comorbidities.</p><p><strong>Conclusion: </strong>This study concludes that the right SCV approach remains a morbidity-safe route for TIVAD implantation in BC patients, as in other malignancies. The study suggests that clinicians can use other implantation routes to avoid catheter-related infection in patients scheduled for neoadjuvant treatment, who also have morbidity, and who are likely to receive postoperative radiotherapy.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 6","pages":"1230-1236"},"PeriodicalIF":1.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of circadian rhythm proteins Rev-Erbα and β in the development of neuronal injury after traumatic brain injury.","authors":"Mahmud E Pençe, Enes Doğan, Halil I Koç, Çiğdem Bayraktaroğlu, Serdar Altunay, Zeynep Balçikanli, Ertuğrul Kiliç, Mustafa Ç Beker","doi":"10.55730/1300-0144.5924","DOIUrl":"10.55730/1300-0144.5924","url":null,"abstract":"<p><strong>Background/aim: </strong>Circadian rhythm proteins (CRPs) play critical roles in both physiological and pathophysiological conditions, including neurodegenerative disorders. As members of CRPs, the nuclear receptors Rev-Erbα/β regulate circadian rhythm particularly by inhibiting Bmal1 protein and are involved in the neuroinflammation and cell death processes. However, their roles in the development of neuronal injury after traumatic brain injury (TBI) were largely unexplored, and so were investigated in the present study.</p><p><strong>Materials and methods: </strong>For the induction of TBI, animals were subjected to the cryogenic model of TBI, which is a commonly used animal model and shares essential similarities with cerebral ischemia in terms of pathophysiological cascades. To assess the impact of Rev-Erb proteins on TBI, both Rev-Erbα and Rev-Erbβ proteins were activated or deactivated, and their expression profiles were determined by western blot analyses. Infarct volume and brain swelling were analyzed by cresyl violet staining. Blood-brain barrier (BBB) permeability was analyzed by immunoglobulin G extravasation. Neuronal survival was analyzed by NeuN immunohistochemistry.</p><p><strong>Results: </strong>Our observations indicate that Rev-Erbβ significantly reduced brain injury after TBI, which was reversed by inhibiting this protein. Not activation but the inhibition of both Rev-Erb proteins increased brain swelling significantly. In addition, both Rev-Erbα and Rev-Erbβ improved BBB permeability and neuronal survival significantly, which were reversed by their inhibitions.</p><p><strong>Conclusion: </strong>Our results show that Rev-Erbα and particularly Rev-Erbβ play significant roles in the development of neuronal injury after TBI. Our findings suggest that Rev-Erb proteins would be a potential therapeutic target for the treatment of neurodegenerative diseases.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 6","pages":"1409-1418"},"PeriodicalIF":1.2,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Achillea millefolium</i> ameliorates doxorubicin-induced renal injury via inhibition of oxidative stress and inflammation in rats.","authors":"Mohammed Shaiea, Yiming Dong, Saleh Alomaisi, Hassan Al-Mahbashi, Guozhong Zhang, Chuan Wang","doi":"10.55730/1300-0144.5922","DOIUrl":"10.55730/1300-0144.5922","url":null,"abstract":"<p><strong>Background/aim: </strong>Doxorubicin (Dox) is a potent anticancer medication. However, due to nephrotoxicity, its clinical application is restricted. <i>Achillea millefolium</i> (AM) is a plant used in traditional medicine to treat several conditions, including kidney disorders. The aim of this work was to investigate the preventative properties of AM extract (AME) and their mechanisms against nephrotoxicity caused by Dox in rats.</p><p><strong>Materials and methods: </strong>The rats were assigned randomly to six groups, including a control group, Dox group (5 mg/kg/week via i.p. for 4 weeks), two groups receiving AME (100 or 200 mg/kg, orally for 28 days), and the last two groups receiving Dox + AME (100 or 200 mg/kg, orally for 4 weeks). After the treatment period concluded, samples of blood and renal tissue were collected for analysis. Serum creatinine, urea, and uric acid levels were used to determine nephrotoxicity biochemically. In renal tissue samples, superoxide dismutase (SOD), catalase, glutathione (GSH), glutathione peroxidase (GPx), total antioxidant capacity (TAC), nitric oxide (NOx), tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and nuclear factor kappa B (NF-κB) were measured. Histopathological analysis of the kidneys was also performed.</p><p><strong>Results: </strong>Dox caused a considerable increase in kidney function parameters and the occurrence of histological changes, which were significantly reversed by AME treatment. Mechanistically, Dox caused renal oxidative stress by raising malondialdehyde and NOx levels while lowering SOD, GSH, GPx, and TAC. It also caused inflammation via the stimulation of proinflammatory cytokines in renal tissues. Conversely, the treatment of AME mitigated Dox-evoked abnormalities in the above-mentioned tests.</p><p><strong>Conclusion: </strong>AME could protect against nephrotoxicity caused by Dox by reducing oxidative stress, stimulating antioxidant mechanisms, and suppressing proinflammatory cytokines, suggesting that AME may be useful as an adjuvant therapy for Dox-induced nephrotoxicity.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 6","pages":"1389-1398"},"PeriodicalIF":1.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe degranulation of mesenteric mast cells in an experimental rat mammary tumor model.","authors":"Senem Esin Yavaş, Özkan Yavaş, Semiha Ersoy, Gürsel Sönmez","doi":"10.55730/1300-0144.5921","DOIUrl":"10.55730/1300-0144.5921","url":null,"abstract":"<p><strong>Background/aim: </strong>Breast cancers are one of the most common cancers in women and are responsible for many deaths worldwide. Mast cells are inflammatory cells. Their role in cancers is controversial, and there is limited data on systemic mast cell activation in cancer cases. This study aimed to evaluate systemic mast cell activation in an experimentally induced rat model of breast cancer.</p><p><strong>Materials and methods: </strong>Sprague Dawley female rats were divided into control (n = 6) and mammary tumor (n = 12) groups. In the tumor group, 20 mg 7,12-dimethylbenz[a]anthracene (DMBA) dissolved in 1 mL cottonseed oil was administered intragastrically by gavage, and the rats were followed daily until their mammary tumors reached 3 cm in diameter. The control group received only cottonseed oil. Paraffin sections obtained from the mammary tumor tissue were subjected to hematoxylin-eosin, toluidine blue staining, and proliferating cell nuclear antigen (PCNA) immunohistochemistry. Mesenteric tissues from each subject were also stained with toluidine blue. The number and activation status of mast cells in mammary tumors and mesenteric tissues were evaluated.</p><p><strong>Results: </strong>Toluidine blue staining showed that activated mast cells were commonly found in tumor tissues. Based on the mesenteric tissue analysis, severe degranulation of the mesenteric mast cells was found in the tumor-induced groups compared to the control group.</p><p><strong>Conclusion: </strong>This study demonstrated for the first time that systemic mast cell activation develops in both tumoral and mesenteric tissues in an experimental cancer model. However, it is not known at which stage of tumor development it occurs.</p>","PeriodicalId":23361,"journal":{"name":"Turkish Journal of Medical Sciences","volume":"54 6","pages":"1381-1388"},"PeriodicalIF":1.2,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}