Smart Materials in Medicine最新文献_第7页

A study involving PC-3 cancer cells and novel carbamate gemini surfactants: Is zeta potential the key to control adhesion to cells? 一项涉及PC-3癌症细胞和新型氨基甲酸酯双子表面活性剂的研究：ζ电位是控制细胞粘附的关键吗？

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2022.09.001

R.V. Pavlov, G.A. Gaynanova, D.M. Kuznetsov, Ya.A. Ivanov, S.K. Amerkhanova, A.P. Lyubina, A.D. Voloshina, L.Ya. Zakharova

{"title":"A study involving PC-3 cancer cells and novel carbamate gemini surfactants: Is zeta potential the key to control adhesion to cells?","authors":"R.V. Pavlov, G.A. Gaynanova, D.M. Kuznetsov, Ya.A. Ivanov, S.K. Amerkhanova, A.P. Lyubina, A.D. Voloshina, L.Ya. Zakharova","doi":"10.1016/j.smaim.2022.09.001","DOIUrl":"10.1016/j.smaim.2022.09.001","url":null,"abstract":"<div><p>Liposome surface potential effect on cellular uptake and cytotoxicity is evaluated using liposomes, modified with cationic lipid DOTAP, a series of cationic gemini surfactants with two carbamate fragments, and an amphiphilic peptide SSRGD. The surfactants used are novel representatives of the gemini family with improved self-assembling activity coupled with potential biodegradable properties and displayed increasing antibacterial activity and cytotoxicity with the shortening of hydrophobic alkyl tails. The longest alkyl tail surfactant, 14-6-14(Et), was the most biocompatible of the series, which was chosen for liposome modification. Prepared liposomes of various compositions are characterized from morphological and physicochemical standpoints in order to optimize their biocompatibility and stability. The carbamate gemini surfactants were also twice as effective at providing positive charge to liposomes and less toxic compared to DOTAP. On their own, carbamate surfactants were able to increase cellular uptake of liposomes by 190%. The mixed composition of 14-6-14(Et) surfactant and SSRGD amphiphilic peptide was the most readily absorbed formulation among different tested neutral, cationic and RGD-modified liposomes. The comparison between the cellular uptake promotion is conducted as to what is the most selective and efficient approach to enhance lipid nanoparticle uptake by cancerous cells.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49657172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Phospholipid-based nanodrill technology for enhanced intracellular delivery of nano-sized cargos 基于磷脂的纳米钻技术用于增强纳米级货物的细胞内递送

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2023.03.001

Doyeon Kim , Seung Soo Nam , Hyunbum Jeon , Youngheun Cho , Eunji Sim , Hyuncheol Kim

{"title":"Phospholipid-based nanodrill technology for enhanced intracellular delivery of nano-sized cargos","authors":"Doyeon Kim , Seung Soo Nam , Hyunbum Jeon , Youngheun Cho , Eunji Sim , Hyuncheol Kim","doi":"10.1016/j.smaim.2023.03.001","DOIUrl":"10.1016/j.smaim.2023.03.001","url":null,"abstract":"<div><p>Nanosized drug delivery systems typically enter the cell via endocytosis. However, a significant amount of the endocytosed cargo cannot effectively escape from the endosome, resulting in drug degradation. Therefore, there are several ongoing efforts to develop transmembrane delivery systems that could circumvent endocytosis. In this study, phospholipid nanotube nanodrills (LDs) were formed onto the surface of a human serum albumin nanoparticle via self-assembling phospholipids. The nanodrill technology enhanced the intracellular uptake efficiency of nanoparticles via energy-independent direct cell membrane permeation. The length of the nanodrills according to the DSPE-PEG to DSPC ratio was investigated both experimentally and theoretically. Our findings demonstrated that longer nanodrills were formed on the surface of the nanoparticles as the ratio of DSPC (i.e., a strongly hydrophobic lipid) in the two phospholipids increases. Moreover, the intracellular uptake efficiency increased as the length of phospholipid nanodrills increased. In addition to enhancing intracellular delivery, the phospholipid nanodrills could penetrate the extracellular matrix and enable the introduction of nanoparticles, thus highlighting the promising tissue penetration capacity of phospholipid nanodrill technology. The improved cell permeability of LD technology was demonstrated by effectively inhibiting specific genes via siRNA-based therapeutic delivery. Moreover, this approach enhanced the efficacy of chemotherapeutics against chemo-resistant cancer cells. Therefore, LD technology could be used to deliver genetic materials and chemical-based therapeutics both <em>in vitro</em> and <em>in vivo</em>.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46998912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing 结构优化的电纺支架用于生物材料控制的协同增强缺陷骨愈合

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2023.05.002

Jeong In Kim , Thi Thu Trang Kieu , Sung-Ho Kook , Jeong-Chae Lee

{"title":"Structurally optimized electrospun scaffold for biomaterial-controlled synergistic enhancement of defective bone healing","authors":"Jeong In Kim , Thi Thu Trang Kieu , Sung-Ho Kook , Jeong-Chae Lee","doi":"10.1016/j.smaim.2023.05.002","DOIUrl":"10.1016/j.smaim.2023.05.002","url":null,"abstract":"<div><p>Bone repair processes are tightly affected by fiber topographies of scaffolds and can be promoted by coupling with chemotactic and/or angiogenic molecules. Here, we developed polycaprolactone (PCL) and collagen-based fibrous scaffolds expressing various architectures via a modified electrospinning set up. We conjugated the as-spun scaffolds with caffeic acid (CA) and/or a cartilage oligomeric matrix protein of angiopoietin 1 (COMP-Ang1). The CA-coupled PCL/collagen scaffold (PCL/col/CA) exhibited greater treatment efficacies for biomimetic and cellular mineralization, expression of osteogenic and chemotactic molecules, and cell migration than did the PCL/col treatment alone. Among the PCL/col/CA scaffolds, the radially symmetric grid-patterned scaffold (rG-PCL/col/CA) showed the greatest bioactivities. The linking of the rG-PCL/col/CA with COMP-Ang1 increased the expression of vascular endothelial growth factor by cells. The COMP-Ang1-linked rG-PCL/col/CA formed more new blood vessels and expressed more chemotactic molecules in a rat model of femoral defects than did the scaffold alone. Compared with PCL/col/CA scaffolds, the COMP-Ang1-coupled rG-PCL/col/CA scaffold stimulated faster and greater healing of femoral defects. Collectively, this study demonstrates that the coupling of a radially grid-patterned fibrous scaffold with CA and COMP-Ang1 greatly enhances scaffold-mediated bone healing via synergistic improvements in vascularization, cell migration, and formation and maturation of new bones in defected regions.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41758349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Current state of art smart coatings for orthopedic implants: A comprehensive review 骨科植入物智能涂层的现状:综合综述

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2023.06.005

Mansi Uday Joshi , Shruti Prakash Kulkarni , Mounika Choppadandi , M. Keerthana , Govinda Kapusetti

引用次数: 3

Nanotherapies from an oncologist doctor's view 肿瘤医生眼中的纳米疗法

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2022.07.005

Shuangqing Liu, Lijun Li, Xinyu Zhang, Qingwei Meng

引用次数: 1

Advances in mRNA nanomedicines for malignant brain tumor therapy 信使核糖核酸纳米药物治疗恶性脑肿瘤的研究进展

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2022.11.001

Ting Deng , Ikram Hasan , Shubham Roy , Yue Liu , Baozhu Zhang , Bing Guo

{"title":"Advances in mRNA nanomedicines for malignant brain tumor therapy","authors":"Ting Deng , Ikram Hasan , Shubham Roy , Yue Liu , Baozhu Zhang , Bing Guo","doi":"10.1016/j.smaim.2022.11.001","DOIUrl":"10.1016/j.smaim.2022.11.001","url":null,"abstract":"<div><p>Nowadays, malignant brain tumors are still mostly lethal diseases with poor prognosis and a clinical median survival rate of fewer than 2 years after therapeutic intervention. It is difficult to achieve complete remission of brain tumors due to blood-brain barrier (BBB) and a lack of efficient drug delivery systems to targeted transportation of brain tumor medicines. Nanoparticle delivery systems have shown merits including stability and high carrier capacity for the transportation of different drugs to treat brain tumors. The application of mRNA nanomedicines brings in great promise not only in COVID-19, but also for malignant brain tumor immunotherapy. The appropriate delivery system facilitates mRNA delivery efficiency and enhances the immune response successfully, for optimal treatment outcomes on malignant brain tumors. Herein, we do an updated review on the development of mRNA nanomedicines for malignant brain cancer treatment. We focus on how to design mRNA-loaded nanoparticle-based delivery systems with optimized pharmacokinetics and pharmacodynamics for efficient therapy of brain cancers. In addition, we point out the challenges and solutions for further development of mRNA nanomedicines for brain cancer therapy. We hope this review would stimulate interest among researchers with different backgrounds and expedite the translation from bench to bedside for the mRNA nanomedicines.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42379087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A responsive hydrogel-based microneedle system for minimally invasive glucose monitoring 用于微创血糖监测的反应性水凝胶微针系统

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2022.07.006

Yan Wang , Haiyang Liu , Xuxia Yang , Zhekun Shi , Jingwen Li , Longjian Xue , Sheng Liu , Yifeng Lei

{"title":"A responsive hydrogel-based microneedle system for minimally invasive glucose monitoring","authors":"Yan Wang , Haiyang Liu , Xuxia Yang , Zhekun Shi , Jingwen Li , Longjian Xue , Sheng Liu , Yifeng Lei","doi":"10.1016/j.smaim.2022.07.006","DOIUrl":"10.1016/j.smaim.2022.07.006","url":null,"abstract":"<div><p>Blood glucose (BG) monitoring in patients with diabetes is critical for diabetes management. Minimally invasive BG monitoring is urgently required to increase the patient compliance. Herein, based on a responsive hydrogel system, we developed a smart microneedle patch system for minimally invasive glucose monitoring. The patch consisted of a transparent substrate of photocurable resin and microneedles made of a pH-responsive and glucose-responsive hydrogel. The responsive hydrogel was composed of a photocrosslinkable hydrogel of gelatin methacrylate (GelMA) together with a pH-responsive nanogel (nano(CMC-pHEA)) and glucose oxidase (GOx). The composite hydrogel showed fast response and high sensitivity to glucose levels in physiological range, mainly due to the ionization of CMC-pHEA component and proton balance. The microneedles showed sufficient mechanical strength to penetrate the skin of mice with minimal invasion, and achieved in situ extraction of glucose in interstitial fluid (ISF) and in situ glucose-responsive reaction. We demonstrated the rapid glucose monitoring by microneedle patch system in skin-mimicking gels <em>in vitro</em> and in diabetic mice <em>in vivo</em>. The microneedles quickly and sensitively responded to glucose concentrations, allowed quantitative readouts of glucose levels through the changes of microneedle heights and swelling ratios. Moreover, the readouts in mice <em>in vivo</em> were consistent with BG levels measured by glucometer. This smart microneedle system has potentials to replace blood sampling, and minimize patient discomfort during BG testing, therefore has potentials in minimally invasive, rapid and reliable BG monitoring.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42530185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

3D biomimetic tumor microenvironment of HCC to visualize the intercellular crosstalk between hepatocytes, hepatic stellate cells, and cancer cells 三维模拟肝癌肿瘤微环境，可视化肝细胞、肝星状细胞和癌细胞之间的细胞间串扰

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2022.12.002

Yaolin Liu , Xiaoqian Yang , Dong Jiang , Rongcheng Hu , Fangli Huang , Xuenong Zou , Chun Liu , Zhenwei Peng

{"title":"3D biomimetic tumor microenvironment of HCC to visualize the intercellular crosstalk between hepatocytes, hepatic stellate cells, and cancer cells","authors":"Yaolin Liu , Xiaoqian Yang , Dong Jiang , Rongcheng Hu , Fangli Huang , Xuenong Zou , Chun Liu , Zhenwei Peng","doi":"10.1016/j.smaim.2022.12.002","DOIUrl":"https://doi.org/10.1016/j.smaim.2022.12.002","url":null,"abstract":"<div><p>While a significant number of studies have focused on elucidating the functioning mechanisms of the Hepatocellular carcinoma (HCC) microenvironment, the intercellular crosstalk between multiple cells in the tumor microenvironment remains unclear. Here we co-cultured spheroids of HCC cells, hepatic stellate cells (HSCs), and hepatocytes in a biomimetic composite hydrogel to construct a 3D model of the HCC microenvironment <em>in vitro</em>. The model reproduced the major cellular components of early HCC in a biomimetic 3D microenvironment, realizing the visualization of the cellular interplay between cells and the microenvironment. Using this model, we showed that the HSCs were activated when co-cultured with HCC cells and deposed collagen to remodel the microenvironment, which in turn triggered higher EMT levels in HCC cells. The hepatocytes also responded to the existence of HCC cells and the activation of HSCs in co-culture, showing the downregulated expression level of ALB, AFP, and HNF4A. This model recapitulated the activation of HSCs in the HCC microenvironment and enabled visualization of multicellular interplay in 3D, providing a biomimetic platform to investigate mechanisms of HCC and related hepatic fibrosis.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49717190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural biopolyester microspheres with diverse structures and surface topologies as micro-devices for biomedical applications 具有多种结构和表面拓扑结构的天然生物聚酯微球作为生物医学应用的微器件

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2022.07.004

Ze-Yu Wang , Xu-Wei Zhang , Yan-Wen Ding , Zi-Wei Ren , Dai-Xu Wei

{"title":"Natural biopolyester microspheres with diverse structures and surface topologies as micro-devices for biomedical applications","authors":"Ze-Yu Wang , Xu-Wei Zhang , Yan-Wen Ding , Zi-Wei Ren , Dai-Xu Wei","doi":"10.1016/j.smaim.2022.07.004","DOIUrl":"10.1016/j.smaim.2022.07.004","url":null,"abstract":"<div><p>Based on their excellent biocompatibility and adjustable biodegradability, the two natural polyesters polylactic acid (PLA) and polyhydroxyalkanoates (PHAs) have been widely used in medical engineering and regenerative medicine. Different types of natural biopolyester microspheres (NBPMs) composed of PLA, PHAs and their derivatives have been designed and used in diverse micro-devices in the last few decades, offering promise for diverse biomedical applications. In addition to biocompatibility and biodegradability, the structure and surface topology of NBPMs also affects <em>in vitro</em> and <em>in vivo</em> cell behaviors such as proliferation, metabolism and differentiation, which are often neglected. In this review, we summarized the preparation methods and properties of diverse NBPMs, including solid, hollow, open porous, and nanofibrous structures, as well as smooth, golf-ball-like, wrinkled, convex, rough and Janus surface topologies, respectively. Moreover, the advantages and limitations of NBPMs for medical applications are analyzed, including tissue engineering (e.g., regeneration of bone, cartilage, liver, tooth, myocardium, and skin), cell engineering for <em>in vitro</em> 3D cell culture, transportation, and cryopreservation, as well as different drug-release models. Finally, we discuss possible future applications of NBPMs with novel, more complex surface structures in light of current trends in biomedicine.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48282140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Towards clinical translation of the cell sheet engineering: Technological aspects 细胞片工程的临床翻译：技术方面

Smart Materials in Medicine Pub Date : 2023-01-01 DOI: 10.1016/j.smaim.2022.09.002

Irina M. Zurina , Victoria S. Presniakova , Denis V. Butnaru , Peter S. Timashev , Yury A. Rochev , Xing-Jie Liang

{"title":"Towards clinical translation of the cell sheet engineering: Technological aspects","authors":"Irina M. Zurina , Victoria S. Presniakova , Denis V. Butnaru , Peter S. Timashev , Yury A. Rochev , Xing-Jie Liang","doi":"10.1016/j.smaim.2022.09.002","DOIUrl":"10.1016/j.smaim.2022.09.002","url":null,"abstract":"<div><p>Cell sheet engineering is a rapidly growing field of tissue engineering and regenerative medicine. The ease of cell sheet obtainment techniques and the resulting unique characteristics and microenvironment of these multicellular structures give rise to the wide range of their <em>in vivo</em> application. At the same time, there are also macroscale cell sheet properties such as thickness, shrinkage after detachment due to cytoskeleton relaxation, and resulting mechanical characteristics. The main topic of this review is the discussion of these properties and how they define the need to use special approaches to manipulating cell sheets during stacking several structures, transferring them to surgical sites, or cryopreserving them. We aimed to systematize the existing techniques of cell sheet transferring, and describe their principles, advantages, and drawbacks regarding cell sheet application during surgical procedures on various tissues and organs. Attention is also paid to such aspects and details as cell sheet positioning <em>in vivo</em>, their ability to spontaneous adhesion, and the requirement for additional fixation at particular surgical sites. Finally, the last section of this review covers the subject of cell sheet cryopreservation – the discussion of freezing and thawing protocols, the variety of cryoprotectants and their mixtures, as well as special requirements such as cryoprotectant loading systems, and cell sheet supporting systems that also stem from their unique macroscale characteristics. Altogether, this systematized review of existing technological approaches related to cell sheet application <em>in vivo</em> can be potentially helpful for the new and expert researchers in this area of tissue engineering.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44427945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2