Platinum nanoparticles enhance osteogenic differentiation of human dental follicle stem cells via scavenging ROS

Q1 Engineering

Smart Materials in Medicine Pub Date : 2023-01-01 DOI:10.1016/j.smaim.2023.06.004

Zheng Wang , Jiaxun Wang , Jiacheng Liu , Yating Zhang , Jingyi Zhang , Ruimeng Yang , Zhaosong Meng , Xiaoqun Gong , Lei Sui

{"title":"Platinum nanoparticles enhance osteogenic differentiation of human dental follicle stem cells via scavenging ROS","authors":"Zheng Wang , Jiaxun Wang , Jiacheng Liu , Yating Zhang , Jingyi Zhang , Ruimeng Yang , Zhaosong Meng , Xiaoqun Gong , Lei Sui","doi":"10.1016/j.smaim.2023.06.004","DOIUrl":null,"url":null,"abstract":"<div><p>The over-accumulation of ROS during prolonged <em>in vitro</em> expansion could negatively affect the properties of stem cells. This leads to a reduced capacity for self-renewal and a lower potential for multiple differentiation, ultimately hindering their applicability in regenerative medicine. Herein, we fabricated platinum nanoparticles (PtNPs) as a potential biocompatible antioxidant to efficiently eliminate the ROS accumulation in human dental follicle stem cells (hDFSCs) during <em>in vitro</em> expansion, thereby enhancing hDFSCs proliferation and osteogenic differentiation. Transcriptome analysis revealed that PI3K/AKT signaling pathway was activated in PtNPs-treated hDFSCs. Transplantation of PtNPs-treated rDFSCs could facilitate new bone formation compared to transplantation of PBS or un-treated rDFSCs, leading to efficient regeneration of bone tissue in rat mandibular bone defect models. In conclusion, PtNPs offered a novel antioxidative strategy to improve stem cell properties and stem-cells-based alveolar bone regeneration.</p></div>","PeriodicalId":22019,"journal":{"name":"Smart Materials in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Smart Materials in Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590183423000212","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Engineering","Score":null,"Total":0}

引用次数: 1

Abstract

The over-accumulation of ROS during prolonged in vitro expansion could negatively affect the properties of stem cells. This leads to a reduced capacity for self-renewal and a lower potential for multiple differentiation, ultimately hindering their applicability in regenerative medicine. Herein, we fabricated platinum nanoparticles (PtNPs) as a potential biocompatible antioxidant to efficiently eliminate the ROS accumulation in human dental follicle stem cells (hDFSCs) during in vitro expansion, thereby enhancing hDFSCs proliferation and osteogenic differentiation. Transcriptome analysis revealed that PI3K/AKT signaling pathway was activated in PtNPs-treated hDFSCs. Transplantation of PtNPs-treated rDFSCs could facilitate new bone formation compared to transplantation of PBS or un-treated rDFSCs, leading to efficient regeneration of bone tissue in rat mandibular bone defect models. In conclusion, PtNPs offered a novel antioxidative strategy to improve stem cell properties and stem-cells-based alveolar bone regeneration.

查看原文本刊更多论文

铂纳米颗粒通过清除活性氧促进人牙滤泡干细胞成骨分化

ROS在长时间体外扩增过程中的过度积累可能会对干细胞的特性产生负面影响。这导致自我更新能力降低，多重分化的潜力降低，最终阻碍了它们在再生医学中的应用。在此，我们制备了铂纳米颗粒（PtNPs），作为一种潜在的生物相容性抗氧化剂，可以在体外扩增过程中有效消除人类牙毛囊干细胞（hDFSCs）中ROS的积累，从而增强hDFSC的增殖和成骨分化。转录组分析显示，在PtNPs处理的hDFSC中，PI3K/AKT信号通路被激活。与PBS或未处理的rDFSCs的移植相比，PtNPs处理的rFSCs的移植可以促进新骨的形成，从而在大鼠下颌骨缺损模型中实现骨组织的有效再生。总之，PtNPs为改善干细胞特性和基于干细胞的牙槽骨再生提供了一种新的抗氧化策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊